Carg22095 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg22095
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	flap endonuclease GEN-like 2
Location	Carg_Chr18: 11339436 .. 11344009 (+)
RNA-Seq Expression	Carg22095
Synteny	Carg22095

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TAAAAGAAAAGCGAAAACTAAGAAATCAAGGACCCCCACAAGCCGAAACCTCTCCGCGGGAGAAATGGGGGTAAAGAACTTATGGGATATCTTAGAATCGTGCAAGAAAACTCTCCCACTTCATCGTCTTCAGTAAGTGGTTTCCTTCATTTTCTCGGCAGCCAAACAGGGGATTTGGGTATTTGTATCTGTATTTTCTTTTGAGTTTTTGGGATTTTGAATTGGGGTTCAGGAATAAGAGGCTATGCATCGATCTCTCATGTTGGATGGTTCAGCTTCAAAATGTAAGCAAATCACATTCTTGTCTGAACCAGAAGATTTACCTGAAGGGGCTCTTTCACCGCCTTAGAGCGCTCATTGCCTTGAATTGCAGCCTTATTTTCGTTACAGGCATGCTCCACATGAAACTACACAGCTATTACGAACAACAAATGATGGGTTCTCGTTTAATTTTGCTTTCTTTTTGCTGTTTGTAGATGGTTCAATTCCTGGCATCAAACTTTCAACTTATAGACGCCGTTTGAACAATGGAAGTGAGGTGGGAATTCTCATAACCCACTTTCGCTTCATTTCTCCTTTTTACTTGAATGTTACTTACCAAAGATGCAACAAGACAGGTTGTTAAAAATGACACAAATTCCCAGCAAATATCCTCACTGAAAAGAAACAAGGGTTCTGAGTTCTCTTTAATGATCAAAGAGGCAAAAGCCTTAGGACTGGCGCTTGGTATCCCTTGTCTAGATGGGTAAATGCTTAAGTTTTCATTCTGTTTTTGGCTTAATGAAAGAGTTCATTCAATTTTCAATCAGAATCTTATGGTTTCCTGTCTCCTGAGCACTTGAATCTTTTTGCATATACCTCTGAATGTAGGCTCGAGGAAGGGGAAGCACAATGTGCATTACTAAATTCAGAGCTATTATGTGTAAGTTGAGTCTTAGTTATAATGTTTGAGTGCTCCACATGCTTATTGCTTTTGCATCTCTGTTGTATTGAACATCATTGCTATTATTAAAGCATCATTTTCTGATCAGGATGGATGTTTTACTTCAGATTCAGATGTTTTCCTTTTTGGTGCGAGGACAGTTTATAGGGACATATGCCTTGGTGAGTGTCCTAGAGCATGCGAAGTTTCTGTAGAGTTGGTTTATGGTTTTAAAAGATGTACTCATTATTCTTATTATTTGTTGTTAAATTAGGAGATGGTGGTCATGTAGTGTGTTATGAAATGGATGACATTGAAGGACAATTGGGCTTTGGAAGGAACTCAATGGTGCGTCTACATTTCGAACAATGTATGATTTAAGAGAGTAGAGTGTTTTTGTGAGATCTCACATTGGTTGGAGAGGTGAACGAATCATTTTTTATAAGGGTGTAGAAACCTCTCCCTAACAGATGCGTTTTAAAACCTTGAGGAGAAAGCCTAGAAGGGAAAGCCTAGAGAAGACAATATCTGCTAGTGGTGAGCTTCGACTGTTACAAATAGTATCAGAATTAGACACCGGGTAGTGCGCCAGCGAGAACGCTGGCCCCCAAGGAGGGTGGATTGTGAGATTTCATATTGTTTGGAGAGGGGAACGAAGCATTCTTTATAAGGTGTGGAAACCCCTCCCTAACAGACACATTTTAAAACCTTGAAGGGAAGCTCGGAAGGGAAAGCCTAGAGAAGACAATATCTGCTAGTGGTGAGCTTCGACTGTTACAAATAGTATCAGAATCAGACACCGGGTAGTGCGCCAGCAAGAACGCTGGCCCCCAAGGAGGGTGGATTGTGAGATTTCATATTGTTTGGAGAGGGGAACGAAGCATTCTTTATAAGGTGTGGAAACCCCTCCCTAACAGACACATTTTAAAACCTTGAAGGGAAGCTCGGAAGGGAAAGCCTAGAGAAGACAATATCTGCTAGTGGTGGGCTTCGGCTGTTACAAATAGTATCAGAATCAGACACCGGGTAGTGCGCCAGCGAGAATGCTAGCCCCCAAGGAGGGTGGATTGTGAGATTCCATATTGTTTGGAGAGGGGAATGAAGCATTCTTTATAAGGTGTGGAAACCCCTCCCTAACAGCGCATTTTAAAACCTTGAAGGGAAGCTCGGAAGGGAAAGATCAAAGAAGACTATATCTGCTAGCGGTGGGTTTGGGCTGTTACAAATGGTATTAGAGCCAGACACCGGGCAATGTGCCAGCCAAGTTGGGTGGATTGTGAGATCCCACATTTGTTGGAGAGAGGAATGAATTCTTTATAAGAGTGTGGAAACATCTCCCTAACAGACGCGTTTTAAAACTTTGAGGGGAAATCTGGAAGGAAAAGACCAAATTGGACAATATCTATTAGCGGTGGGCTTGGGCTGTTAGTGTTTCACTTGAAGGAAAGATGAGCTTTCTCTTTCTATGATCAAATATGTTATCATTACCAAATCATTTTGTGATTTCCAGATTGCTTTGGCCCTACTTCTTGGCAGTGACTACACGCAAGGCGTTTATGGCATGGGTCGTGTAGGTTTCAACTCCTGTTTTTCTCTATTGGATTGCTTTTTATACTAACAATTCGACTGCAGCATGTAACTGTGTCTATGCTGTTTTTGTACTCTTTGTAGGAATCTGCTCGTCAGATTGTTAAAACAGTAGGAGATAGCACTGTCCTTCAAAAAATTGCATCTGAAGGACTGTCTTTCACAATGAAGGGGAAAAATTCCAAGAAGCCAGGACTTGCTAGTTCTGGTCATATTACTTCTATCAAGCTTCATTCTCCTTTTTCAACACTAAAAGAAGTGGAATAGGGGAATTTGAGCCTTGGACAGAAACTAAAAAACAACCTTCTTAGCCTCACTAGTGGGCTTAGGAACTTAAGTAGTGCAGTTTTGATACTATTCTGATATGATAGGTCAATCAAGTTTTGATTTGTGATGTGGGTTACTTTGGTTTGGTTGTACAGGGGATGGGCAGTACATTCATAAACGTGAGGAGTTTTCACAAGTCATTAATGCCTATTTGAGACCCAAATGCCATTCAGCTGATTCTGATGCTGTGACTAGGTGAGCTTGTTGAACCTTTGGAATTACATTACAAATAGTTCTGTACTCTGTGTTTGTGTTTGCAAAAGAGATCTCACATTGGTTGGAGAGGAGAACGAAACATTCCTTACAAGGGTATAGAAATCTCCCCCTAGAAAACGTGTTTTAAAACTATGAGGTTGATGGTGATACATAACGGGCCAGAACAGACAACATCTGTTAGCGCTGGGCTGCTTCGTTGTAATTTTGTGTCATACTGATATTTACACGATTAGTCTTCAAACTTTCCTTTTTGAAATGCATGTAGATTTTGTTAAATTCATATGCCTTCAGCACTTTTTATTTTTCTCTACAGGGTTCTTATTCAGCATTCTTTTCAACGTGCCAAGCTTCAGCAGTTCTGTGCCGAATTTTTTGCTTGGCCTCCTGAGAAAACAGGTTCTTTTTTTTTTCCTTCATCTTTGGAGCTAACTAGAAAATGGTTCCTTTGATGTTTAGCTTGTGAATAACCCATGCATGGTTCAGATGAGTACATTCTTCCAAAGATAGCAGAAAAAGATCTACGACGATTTGCTAATCTTCGTTCGAACACATCAGACCTCGGTTTCAACATTCCACTTCAAGAGGTATGTTTATTTCTTCAAGAGTTACTTTTATCAGTTGTTACTGATATGGATACTGTTTGATTGCATGTTTTTTATTACAGGTACCTGTAAGTTGTCCTGTTTCTGGAATCGTCAAGCACCGAAAAGTCCAGGGGAACGAATGCTATGAGGTTTCATGGAAAAATTTAGATGGGCTTAGTTCATCTGTTGTACCAGCAGATCTCTTGCAGAGGTATGGAGTGTCATTACATAATTTCTTCTATAACAAATGACGATTTGGATACTTCACAACTATTTCTTTTTGAATATTTCCACAGTGCCTGTCCAGAGATGATTGTAGAATTTGAAGAGCAAAGAGCTCAAGGAAGAAAACCAAATAAGTGCAGAACAAAAACAAAGAAGTCAGAGGCTACTGTGGCTGAAATTGATGAAAGATTCCGAACTCTGTTGCTTGAAATCGAATCTGAAAGCAGGGCGGTTCGAAATCTTTCCCAAGCTCCAATAGTTTCGGAAAACAGTAACTCTGCTACTGGTTTGGATGATTTGCAAGAGATTATTCTTGACGTCGAACCAACCATTGTTGATCATGCTCACGGTACAGAGAACGTTGAGGTCATCAATCTCTTAAGCCCTTCACCTGCAATTCAAACGCGCAAAGTTTCGAAATTTCGACAGGGAAACTGTGAAAAGATTGATGTAATTGATTTGAGTGACACAGAAAATGATCAGTCACCTGAACATGAGAGAAAGGCAAGAGAGTTGAGATTGTTCTTAGCCTCCTTAAAGGGTAAATGACTTCCTCTGATAGGCCATAAATGTATTATGTATATTGCAACGAAATGTGCAACGCTACCGAAGGAATTAGGATTTAGAATCTGGTTTTTAGATATTGTCTGCTTTGACCCGTTACGTATCGCCGTCAGCTAGGGAGAGATTTCCACACATTTATAAGAAATAAT

mRNA sequence

TAAAAGAAAAGCGAAAACTAAGAAATCAAGGACCCCCACAAGCCGAAACCTCTCCGCGGGAGAAATGGGGGTAAAGAACTTATGGGATATCTTAGAATCGTGCAAGAAAACTCTCCCACTTCATCGTCTTCAGTAAGTGGTTTCCTTCATTTTCTCGGCAGCCAAACAGGGGATTTGGGTATTTGTATCTGTATTTTCTTTTGAGTTTTTGGGATTTTGAATTGGGGTTCAGGAATAAGAGGCTATGCATCGATCTCTCATGTTGGATGGTTCAGCTTCAAAATGTAAGCAAATCACATTCTTGTCTGAACCAGAAGATTTACCTGAAGGGGCTCTTTCACCGCCTTAGAGCGCTCATTGCCTTGAATTGCAGCCTTATTTTCGTTACAGATGGTTCAATTCCTGGCATCAAACTTTCAACTTATAGACGCCGTTTGAACAATGGAAGTGAGGTTGTTAAAAATGACACAAATTCCCAGCAAATATCCTCACTGAAAAGAAACAAGGGTTCTGAGTTCTCTTTAATGATCAAAGAGGCAAAAGCCTTAGGACTGGCGCTTGGTATCCCTTGTCTAGATGGGCTCGAGGAAGGGGAAGCACAATGTGCATTACTAAATTCAGAGCTATTATGTGATGGATGTTTTACTTCAGATTCAGATGTTTTCCTTTTTGGTGCGAGGACAGTTTATAGGGACATATGCCTTGGTGAGTGTCCTAGAGCATGCGAAGTTTCTGTAGAGTTGATTGCTTTGGCCCTACTTCTTGGCAGTGACTACACGCAAGGCGTTTATGGCATGGGTCGTGAATCTGCTCGTCAGATTGTTAAAACAGTAGGAGATAGCACTGTCCTTCAAAAAATTGCATCTGAAGGACTGTCTTTCACAATGAAGGGGAAAAATTCCAAGAAGCCAGGACTTGCTAGTTCTGGGGATGGGCAGTACATTCATAAACCACTTTTTATTTTTCTCTACAGGGTTCTTATTCAGCATTCTTTTCAACGTGCCAAGCTTCAGCAGTTCTGTGCCGAATTTTTTGCTTGGCCTCCTGAGAAAACAGATGAGTACATTCTTCCAAAGATAGCAGAAAAAGATCTACGACGATTTGCTAATCTTCGTTCGAACACATCAGACCTCGGTTTCAACATTCCACTTCAAGAGGTACCTGTAAGTTGTCCTGTTTCTGGAATCGTCAAGCACCGAAAAGTCCAGGGGAACGAATGCTATGAGGTTTCATGGAAAAATTTAGATGGGCTTAGTTCATCTGTTGTACCAGCAGATCTCTTGCAGAGTGCCTGTCCAGAGATGATTGTAGAATTTGAAGAGCAAAGAGCTCAAGGAAGAAAACCAAATAAGTGCAGAACAAAAACAAAGAAGTCAGAGGCTACTGTGGCTGAAATTGATGAAAGATTCCGAACTCTGTTGCTTGAAATCGAATCTGAAAGCAGGGCGGTTCGAAATCTTTCCCAAGCTCCAATAGTTTCGGAAAACAGTAACTCTGCTACTGGTTTGGATGATTTGCAAGAGATTATTCTTGACGTCGAACCAACCATTGTTGATCATGCTCACGGTACAGAGAACGTTGAGGTCATCAATCTCTTAAGCCCTTCACCTGCAATTCAAACGCGCAAAGTTTCGAAATTTCGACAGGGAAACTGTGAAAAGATTGATGTAATTGATTTGAGTGACACAGAAAATGATCAGTCACCTGAACATGAGAGAAAGGCAAGAGAGTTGAGATTGTTCTTAGCCTCCTTAAAGGGTAAATGACTTCCTCTGATAGGCCATAAATGTATTATGTATATTGCAACGAAATGTGCAACGCTACCGAAGGAATTAGGATTTAGAATCTGGTTTTTAGATATTGTCTGCTTTGACCCGTTACGTATCGCCGTCAGCTAGGGAGAGATTTCCACACATTTATAAGAAATAAT

Coding sequence (CDS)

ATGGTTCAGCTTCAAAATGTAAGCAAATCACATTCTTGTCTGAACCAGAAGATTTACCTGAAGGGGCTCTTTCACCGCCTTAGAGCGCTCATTGCCTTGAATTGCAGCCTTATTTTCGTTACAGATGGTTCAATTCCTGGCATCAAACTTTCAACTTATAGACGCCGTTTGAACAATGGAAGTGAGGTTGTTAAAAATGACACAAATTCCCAGCAAATATCCTCACTGAAAAGAAACAAGGGTTCTGAGTTCTCTTTAATGATCAAAGAGGCAAAAGCCTTAGGACTGGCGCTTGGTATCCCTTGTCTAGATGGGCTCGAGGAAGGGGAAGCACAATGTGCATTACTAAATTCAGAGCTATTATGTGATGGATGTTTTACTTCAGATTCAGATGTTTTCCTTTTTGGTGCGAGGACAGTTTATAGGGACATATGCCTTGGTGAGTGTCCTAGAGCATGCGAAGTTTCTGTAGAGTTGATTGCTTTGGCCCTACTTCTTGGCAGTGACTACACGCAAGGCGTTTATGGCATGGGTCGTGAATCTGCTCGTCAGATTGTTAAAACAGTAGGAGATAGCACTGTCCTTCAAAAAATTGCATCTGAAGGACTGTCTTTCACAATGAAGGGGAAAAATTCCAAGAAGCCAGGACTTGCTAGTTCTGGGGATGGGCAGTACATTCATAAACCACTTTTTATTTTTCTCTACAGGGTTCTTATTCAGCATTCTTTTCAACGTGCCAAGCTTCAGCAGTTCTGTGCCGAATTTTTTGCTTGGCCTCCTGAGAAAACAGATGAGTACATTCTTCCAAAGATAGCAGAAAAAGATCTACGACGATTTGCTAATCTTCGTTCGAACACATCAGACCTCGGTTTCAACATTCCACTTCAAGAGGTACCTGTAAGTTGTCCTGTTTCTGGAATCGTCAAGCACCGAAAAGTCCAGGGGAACGAATGCTATGAGGTTTCATGGAAAAATTTAGATGGGCTTAGTTCATCTGTTGTACCAGCAGATCTCTTGCAGAGTGCCTGTCCAGAGATGATTGTAGAATTTGAAGAGCAAAGAGCTCAAGGAAGAAAACCAAATAAGTGCAGAACAAAAACAAAGAAGTCAGAGGCTACTGTGGCTGAAATTGATGAAAGATTCCGAACTCTGTTGCTTGAAATCGAATCTGAAAGCAGGGCGGTTCGAAATCTTTCCCAAGCTCCAATAGTTTCGGAAAACAGTAACTCTGCTACTGGTTTGGATGATTTGCAAGAGATTATTCTTGACGTCGAACCAACCATTGTTGATCATGCTCACGGTACAGAGAACGTTGAGGTCATCAATCTCTTAAGCCCTTCACCTGCAATTCAAACGCGCAAAGTTTCGAAATTTCGACAGGGAAACTGTGAAAAGATTGATGTAATTGATTTGAGTGACACAGAAAATGATCAGTCACCTGAACATGAGAGAAAGGCAAGAGAGTTGAGATTGTTCTTAGCCTCCTTAAAGGGTAAATGA

Protein sequence

MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNGSEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDVFLFGARTVYRDICLGECPRACEVSVELIALALLLGSDYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHKPLFIFLYRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIESESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK

Homology

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BLAST of Carg22095 vs. NCBI nr
Match: KAG7013227.1 (Flap endonuclease GEN-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 980.3 bits (2533), Expect = 6.1e-282
Identity = 499/499 (100.00%), Postives = 499/499 (100.00%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL
Sbjct: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGECPRACEVSVELIALALLLGSDYTQGVYGMGRE 180
           LCDGCFTSDSDVFLFGARTVYRDICLGECPRACEVSVELIALALLLGSDYTQGVYGMGRE
Sbjct: 121 LCDGCFTSDSDVFLFGARTVYRDICLGECPRACEVSVELIALALLLGSDYTQGVYGMGRE 180

Query: 181 SARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHKPLFIFLYRVLIQ 240
           SARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHKPLFIFLYRVLIQ
Sbjct: 181 SARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHKPLFIFLYRVLIQ 240

Query: 241 HSFQRAKLQQFCAEFFAWPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQEVPV 300
           HSFQRAKLQQFCAEFFAWPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQEVPV
Sbjct: 241 HSFQRAKLQQFCAEFFAWPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQEVPV 300

Query: 301 SCPVSGIVKHRKVQGNECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKP 360
           SCPVSGIVKHRKVQGNECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKP
Sbjct: 301 SCPVSGIVKHRKVQGNECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKP 360

Query: 361 NKCRTKTKKSEATVAEIDERFRTLLLEIESESRAVRNLSQAPIVSENSNSATGLDDLQEI 420
           NKCRTKTKKSEATVAEIDERFRTLLLEIESESRAVRNLSQAPIVSENSNSATGLDDLQEI
Sbjct: 361 NKCRTKTKKSEATVAEIDERFRTLLLEIESESRAVRNLSQAPIVSENSNSATGLDDLQEI 420

Query: 421 ILDVEPTIVDHAHGTENVEVINLLSPSPAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSP 480
           ILDVEPTIVDHAHGTENVEVINLLSPSPAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSP
Sbjct: 421 ILDVEPTIVDHAHGTENVEVINLLSPSPAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSP 480

Query: 481 EHERKARELRLFLASLKGK 500
           EHERKARELRLFLASLKGK
Sbjct: 481 EHERKARELRLFLASLKGK 499

[Close]

BLAST of Carg22095 vs. NCBI nr
Match: KAG6574173.1 (Flap endonuclease GEN-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 915.6 bits (2365), Expect = 1.8e-262
Identity = 482/529 (91.12%), Postives = 488/529 (92.25%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 35  MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL
Sbjct: 95  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDICLG+     C    ++  +L       IALALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDICLGDGGHVVCYEMDDIEGQLGFGRNSMIALALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQY+HK
Sbjct: 215 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYMHK 274

Query: 241 ----PLFIFLY--------------RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                  I  Y              RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK
Sbjct: 275 CEEFSQVINAYLRPKCHSADSDAVTRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS
Sbjct: 335 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SSVVPADLLQSACPEMIVEFE+QRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES
Sbjct: 395 SSVVPADLLQSACPEMIVEFEQQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 480
           ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI
Sbjct: 455 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 514

Query: 481 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK
Sbjct: 515 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 563

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BLAST of Carg22095 vs. NCBI nr
Match: XP_022944807.1 (flap endonuclease GEN-like 2 [Cucurbita moschata])

HSP 1 Score: 911.0 bits (2353), Expect = 4.5e-261
Identity = 479/529 (90.55%), Postives = 487/529 (92.06%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 35  MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL
Sbjct: 95  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDICLG+     C    ++  +L       IALALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDICLGDGGHVVCYEMDDIEGQLGFGRNSMIALALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLS+TMKGKNSKKPGL +SGDGQY+HK
Sbjct: 215 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSYTMKGKNSKKPGLPNSGDGQYMHK 274

Query: 241 ----PLFIFLY--------------RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                  I  Y              RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK
Sbjct: 275 CEEFSQVIDAYLRPKCHSADSDAVTRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS
Sbjct: 335 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES
Sbjct: 395 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 480
           ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI
Sbjct: 455 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 514

Query: 481 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           QTRKVSKF+QGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK
Sbjct: 515 QTRKVSKFQQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 563

[Close]

BLAST of Carg22095 vs. NCBI nr
Match: XP_023541410.1 (flap endonuclease GEN-like 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 877.9 bits (2267), Expect = 4.3e-251
Identity = 466/529 (88.09%), Postives = 478/529 (90.36%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 35  MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGL LGIPCLDGLEEGEAQCALLNSEL
Sbjct: 95  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLTLGIPCLDGLEEGEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDI LG+     C    ++  +L       IALALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDIGLGDGGHVVCYEMDDIERQLGFGRNSMIALALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DY+QGVYGMGRESA QIVK VGDSTVLQKIASEGLSFT KGKNSKKPGL +SGDGQY+HK
Sbjct: 215 DYSQGVYGMGRESACQIVKAVGDSTVLQKIASEGLSFTKKGKNSKKPGLRNSGDGQYMHK 274

Query: 241 ----PLFIFLY--------------RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                  I  Y              RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK
Sbjct: 275 CEEFSQVIDAYLRPKCHSADSDAVTRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAEKDLRRFANLRSNTS+LGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS
Sbjct: 335 IAEKDLRRFANLRSNTSNLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERF+TLLLEIES
Sbjct: 395 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFQTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 480
           ESRAVRNLSQAPIVSENSNSATGLD+LQ+IILDVEPTIVDHAHGTENVEVI+LLSPSPAI
Sbjct: 455 ESRAVRNLSQAPIVSENSNSATGLDELQDIILDVEPTIVDHAHGTENVEVIDLLSPSPAI 514

Query: 481 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           QT    KFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK
Sbjct: 515 QT---CKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 560

[Close]

BLAST of Carg22095 vs. NCBI nr
Match: XP_022968219.1 (flap endonuclease GEN-like 2 [Cucurbita maxima])

HSP 1 Score: 875.2 bits (2260), Expect = 2.8e-250
Identity = 460/529 (86.96%), Postives = 478/529 (90.36%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTY+RRLNNG
Sbjct: 35  MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYKRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRN+GSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL
Sbjct: 95  SEVVKNDTNSQQISSLKRNQGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDICLG+     C    ++  +L       I+LALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDICLGDGGHVVCYEMDDIERQLGFGRNSMISLALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DY+QGVYGMGRESA QIVKTVGDSTVLQKIASEGLSFT KGKNSKK GL +SGDGQ++HK
Sbjct: 215 DYSQGVYGMGRESACQIVKTVGDSTVLQKIASEGLSFTKKGKNSKKSGLPNSGDGQFMHK 274

Query: 241 ----PLFIFLY--------------RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                  I  Y              RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK
Sbjct: 275 CEEFSQVIDAYLRPKCHSADSDAVTRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAEKDLRRFANLRSNT+ LGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS
Sbjct: 335 IAEKDLRRFANLRSNTAGLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKC+TKTKKSEATVAEID+RF+TLLLEIES
Sbjct: 395 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCKTKTKKSEATVAEIDKRFQTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 480
           ESRAVRNLSQAPIVSENSNSATGLD+LQ+II DVEPTIVDHAHGTE VEVINLLSPSPAI
Sbjct: 455 ESRAVRNLSQAPIVSENSNSATGLDELQDIIFDVEPTIVDHAHGTEIVEVINLLSPSPAI 514

Query: 481 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           QTRKV KF+QGNCEKIDVIDLSDTENDQSPEHERKA ELRLFL SLKGK
Sbjct: 515 QTRKVPKFQQGNCEKIDVIDLSDTENDQSPEHERKASELRLFLTSLKGK 563

[Close]

BLAST of Carg22095 vs. ExPASy Swiss-Prot
Match: Q9M2Z3 (Single-strand DNA endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=SEND1 PE=1 SV=2)

HSP 1 Score: 426.8 bits (1096), Expect = 3.4e-118
Identity = 252/564 (44.68%), Postives = 352/564 (62.41%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MV+L  V+KS+    +K+YL+G FHRLRALIALNCS+I V+DG+IPGIK+ TY+RRL   
Sbjct: 35  MVELHKVNKSYCATKEKVYLRGFFHRLRALIALNCSIILVSDGAIPGIKVPTYKRRLKAR 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
            E+  +     + +SLKRN GSEFS +IKEAK +   LGI CLDG+EE EAQCALLNSE 
Sbjct: 95  FEIADDGVEPSKETSLKRNMGSEFSCIIKEAKVIASTLGILCLDGIEEAEAQCALLNSES 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVE-------LIALALLLGS 180
           LCD CF+ DSD+FLFGA+TVYR+ICLGE     C    ++  +       LIALALLLGS
Sbjct: 155 LCDACFSFDSDIFLFGAKTVYREICLGEGGYVVCYEMDDIKKKLGLGRNSLIALALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKK-------------P 240
           DY+QGV G+ +E A ++V+++GD+ +L+K+ASEGLSF  K + SKK             P
Sbjct: 215 DYSQGVRGLRQEKACELVRSIGDNVILEKVASEGLSFAEKPRKSKKQVRPSVCSKKGTLP 274

Query: 241 GLASSGDG------QYIHKPLFIFL------------YRVLIQHSFQRAKLQQFCAEFFA 300
            +  +G+       + I + +  F+             R L + SFQR KLQ+ C +FF 
Sbjct: 275 LVVINGNNRDPERLEEIKQVIDAFMNPKCHQADSNTVSRALAEFSFQRTKLQEICHQFFE 334

Query: 301 WPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQ--EVPVSCPVSGIVKHRKVQG 360
           WPPEKTDEYILPK+AE++LRRFANL+S ++++  N+PL   ++P  CPVS I+K RKVQG
Sbjct: 335 WPPEKTDEYILPKVAERNLRRFANLQSRSTEVEVNLPLHKPQMPEKCPVSEIIKTRKVQG 394

Query: 361 NECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVA 420
            EC+EVSW +L+GL SS+VPADL++ ACPE I+EF+E+ A  +K  K + K K++ +   
Sbjct: 395 RECFEVSWNDLEGLESSIVPADLVERACPEKIIEFKEKMAAKKKKPKPKQKQKETSSPTK 454

Query: 421 E---IDERFRTLLLEIESESRAVRN-LSQAPIVSENSNSATGLDDLQEIILDVEPTIVDH 480
               ++       L++ S S   R+ L +A   +E  NS     D   +I   +    ++
Sbjct: 455 SSSLVELSLELQHLDLNSTSLVSRSTLEEAEQENEQQNSKK--HDYLRLIDSPDRENCNN 514

Query: 481 AHGTEN--------------VEVINLLSPSPAIQTRKVSKFRQ----GNCEKIDVIDLSD 498
           A    +               EVI+L+SP P  ++R VS+  Q     + +   VI+LSD
Sbjct: 515 AWSNRDRLGVGMSSFPLYPETEVIDLISPCPEARSRSVSRSYQEQKSHDHQLETVIELSD 574

[Close]

BLAST of Carg22095 vs. ExPASy Swiss-Prot
Match: Q8W5R1 (Single-strand DNA endonuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SEND1 PE=2 SV=1)

HSP 1 Score: 412.1 bits (1058), Expect = 8.7e-114
Identity = 235/497 (47.28%), Postives = 311/497 (62.58%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           +VQ+ + ++S +    K+YLK LFHR+RAL+ALNC+L+FVTDG+IP +KL+TYRRRL + 
Sbjct: 35  LVQMYSANRSPAFAKDKVYLKNLFHRIRALLALNCTLLFVTDGAIPSLKLATYRRRLGSI 94

Query: 61  SEVVK--NDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNS 120
           S   K  +  NS    SL+RNKGSEFS MIKEAK LG+ALGIPCLDGLEE EAQCA L+ 
Sbjct: 95  SHAAKESDQPNSHPSISLRRNKGSEFSCMIKEAKRLGMALGIPCLDGLEEAEAQCASLDL 154

Query: 121 ELLCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSV-------ELIALALLL 180
           E LCDGCFTSDSD FLFGARTVYRD+ +GE     C    ++          LI+LA+LL
Sbjct: 155 ESLCDGCFTSDSDAFLFGARTVYRDVFIGEGGYVICYEMEDIEKTLGFGRNSLISLAVLL 214

Query: 181 GSDYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFT--MKGKNSK----------- 240
           GSDY+ GV G G E+A ++VK+VGD+ +L +I S G+  T   KGKNS            
Sbjct: 215 GSDYSNGVNGFGPETACRLVKSVGDNLILDQILSNGVKATRKCKGKNSGNKVDDMCPKAS 274

Query: 241 --KPGLASSGDGQY-----------IHKPLFIFLYRVLIQHSFQRAKLQQFCAEFFAWPP 300
             + G+    DGQ+            H P    + RV  QH F R +LQ+ C E+F W P
Sbjct: 275 SCEVGMTQDSDGQFRDVINAYLEPKCHSPDSEAVQRVCGQHPFLRPQLQKICEEYFDWSP 334

Query: 301 EKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYE 360
           EKTD+YILPKIAE++LRRF++LRS +S LG    L E+PV CPV  IVK RKV GNECYE
Sbjct: 335 EKTDQYILPKIAERELRRFSDLRSASSALGIKPLLSEIPVPCPVLAIVKQRKVHGNECYE 394

Query: 361 VSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDER 420
           VSW+N++GL  SVVP DL++SACPE I EF E++ + +K  +     K  +A V ++DE+
Sbjct: 395 VSWRNIEGLQVSVVPGDLVKSACPEKITEFLEKKGEEKKQKRRARPKKSGQAAVKDVDEQ 454

Query: 421 FRTLLLEIESESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEV 458
            + LLL IE++S  +   + +   +  +     ++D                       V
Sbjct: 455 LQELLLGIEADSGGILGATASVCQTLTAAYTVAVED-----------------------V 506

[Close]

BLAST of Carg22095 vs. ExPASy Swiss-Prot
Match: Q17RS7 (Flap endonuclease GEN homolog 1 OS=Homo sapiens OX=9606 GN=GEN1 PE=1 SV=2)

HSP 1 Score: 90.9 bits (224), Expect = 4.4e-17
Identity = 62/195 (31.79%), Postives = 100/195 (51.28%), Query Frame = 0


Query: 17  KIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNGSEVVKNDTNSQQISSL 76
           K +L+ LF R+  L  ++  L+FV +G  P +K     +R  N S        S   S  
Sbjct: 50  KPHLRNLFFRISYLTQMDVKLVFVMEGEPPKLKADVISKR--NQSRY-----GSSGKSWS 109

Query: 77  KRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDVFLFG 136
           ++   S F  +++E   +   LGIP +    E EA CA LN+    DGC T+D D FL+G
Sbjct: 110 QKTGRSHFKSVLRECLHMLECLGIPWVQAAGEAEAMCAYLNAGGHVDGCLTNDGDTFLYG 169

Query: 137 ARTVYRDICLG------ECPRACEVSVE-------LIALALLLGSDY-TQGVYGMGRESA 196
           A+TVYR+  +       +C     +  +       L+ LA+LLG DY  +GV G+G+E A
Sbjct: 170 AQTVYRNFTMNTKDPHVDCYTMSSIKSKLGLDRDALVGLAILLGCDYLPKGVPGVGKEQA 229

Query: 197 RQIVKTVGDSTVLQK 198
            ++++ +   ++LQ+
Sbjct: 230 LKLIQILKGQSLLQR 237

[Close]

BLAST of Carg22095 vs. ExPASy Swiss-Prot
Match: Q8BMI4 (Flap endonuclease GEN homolog 1 OS=Mus musculus OX=10090 GN=Gen1 PE=1 SV=2)

HSP 1 Score: 90.1 bits (222), Expect = 7.5e-17
Identity = 59/196 (30.10%), Postives = 98/196 (50.00%), Query Frame = 0


Query: 16  QKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNGSEVVKNDTNSQQISS 75
           +K +L+ LF R+  L  +N  L+FV +G  P +K     +R        +        S 
Sbjct: 49  KKPHLRNLFFRISYLTQMNVKLVFVMEGEPPMLKADVISKR-------TQTRYGPSGKSR 108

Query: 76  LKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDVFLF 135
            ++   S F  +++E   +   LG+P +    E EA CA LN+    DGC T+D D FL+
Sbjct: 109 SQKTGRSHFKSVLRECLEMLECLGMPWVQAAGEAEAMCAYLNASGHVDGCLTNDGDAFLY 168

Query: 136 GARTVYRDICLG------ECPRACEVSVE-------LIALALLLGSDY-TQGVYGMGRES 195
           GA+TVYR+  +       +C     +  +       L+ LA+LLG DY  +GV G+G+E 
Sbjct: 169 GAQTVYRNFTMNTKDPHVDCYTISSIKSKLGLDRDALVGLAVLLGCDYLPKGVPGVGKEQ 228

Query: 196 ARQIVKTVGDSTVLQK 198
           A ++++     ++LQ+
Sbjct: 229 ALKLLQIFKGQSLLQR 237

[Close]

BLAST of Carg22095 vs. ExPASy Swiss-Prot
Match: P61942 (Flap endonuclease 1 OS=Nanoarchaeum equitans (strain Kin4-M) OX=228908 GN=fen PE=3 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 6.4e-16
Identity = 64/202 (31.68%), Postives = 98/202 (48.51%), Query Frame = 0


Query: 19  YLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRR------LNNGSEVVKNDTNSQQ 78
           +L GLF+R   L+      I+V DG+ P  K+  + +R      L +  +      N Q+
Sbjct: 58  HLSGLFYRTINLLEYGIKPIYVFDGTPPKFKIVAWEKRKKHKEQLESKYKEALKKGNIQE 117

Query: 79  ISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDV 138
                ++ G   S M++EAK L  A+GIP +    EGEA+ A L  + + D C + D D 
Sbjct: 118 AIKYAKSLGKLDSYMVEEAKKLLEAMGIPYVQAPSEGEAEAAYLTKKGVSDYCGSQDYDS 177

Query: 139 FLFGARTVYRDICLGE-------------CPRACEVSV----------ELIALALLLGSD 192
            LFG+  V R+I + E              P   E+            +LIA+A+LLG+D
Sbjct: 178 LLFGSPRVVRNITISEKRKLPGKNIYVEVKPEVIELEAVLNYWKITREQLIAIAMLLGTD 237

[Close]

BLAST of Carg22095 vs. ExPASy TrEMBL
Match: A0A6J1FZ58 (flap endonuclease GEN-like 2 OS=Cucurbita moschata OX=3662 GN=LOC111449235 PE=4 SV=1)

HSP 1 Score: 911.0 bits (2353), Expect = 2.2e-261
Identity = 479/529 (90.55%), Postives = 487/529 (92.06%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 35  MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL
Sbjct: 95  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDICLG+     C    ++  +L       IALALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDICLGDGGHVVCYEMDDIEGQLGFGRNSMIALALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLS+TMKGKNSKKPGL +SGDGQY+HK
Sbjct: 215 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSYTMKGKNSKKPGLPNSGDGQYMHK 274

Query: 241 ----PLFIFLY--------------RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                  I  Y              RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK
Sbjct: 275 CEEFSQVIDAYLRPKCHSADSDAVTRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS
Sbjct: 335 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES
Sbjct: 395 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 480
           ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI
Sbjct: 455 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 514

Query: 481 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           QTRKVSKF+QGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK
Sbjct: 515 QTRKVSKFQQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 563

[Close]

BLAST of Carg22095 vs. ExPASy TrEMBL
Match: A0A6J1HZ11 (flap endonuclease GEN-like 2 OS=Cucurbita maxima OX=3661 GN=LOC111467522 PE=4 SV=1)

HSP 1 Score: 875.2 bits (2260), Expect = 1.3e-250
Identity = 460/529 (86.96%), Postives = 478/529 (90.36%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTY+RRLNNG
Sbjct: 35  MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYKRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEVVKNDTNSQQISSLKRN+GSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL
Sbjct: 95  SEVVKNDTNSQQISSLKRNQGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDICLG+     C    ++  +L       I+LALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDICLGDGGHVVCYEMDDIERQLGFGRNSMISLALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DY+QGVYGMGRESA QIVKTVGDSTVLQKIASEGLSFT KGKNSKK GL +SGDGQ++HK
Sbjct: 215 DYSQGVYGMGRESACQIVKTVGDSTVLQKIASEGLSFTKKGKNSKKSGLPNSGDGQFMHK 274

Query: 241 ----PLFIFLY--------------RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                  I  Y              RVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK
Sbjct: 275 CEEFSQVIDAYLRPKCHSADSDAVTRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAEKDLRRFANLRSNT+ LGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS
Sbjct: 335 IAEKDLRRFANLRSNTAGLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKC+TKTKKSEATVAEID+RF+TLLLEIES
Sbjct: 395 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCKTKTKKSEATVAEIDKRFQTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDLQEIILDVEPTIVDHAHGTENVEVINLLSPSPAI 480
           ESRAVRNLSQAPIVSENSNSATGLD+LQ+II DVEPTIVDHAHGTE VEVINLLSPSPAI
Sbjct: 455 ESRAVRNLSQAPIVSENSNSATGLDELQDIIFDVEPTIVDHAHGTEIVEVINLLSPSPAI 514

Query: 481 QTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           QTRKV KF+QGNCEKIDVIDLSDTENDQSPEHERKA ELRLFL SLKGK
Sbjct: 515 QTRKVPKFQQGNCEKIDVIDLSDTENDQSPEHERKASELRLFLTSLKGK 563

[Close]

BLAST of Carg22095 vs. ExPASy TrEMBL
Match: A0A6J1CIZ7 (flap endonuclease GEN-like 2 OS=Momordica charantia OX=3673 GN=LOC111011427 PE=4 SV=1)

HSP 1 Score: 758.1 bits (1956), Expect = 2.4e-215
Identity = 398/532 (74.81%), Postives = 442/532 (83.08%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MVQLQNVSKSHSCL++KIYL+GLFHRLRALIALNCS+IFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 35  MVQLQNVSKSHSCLHKKIYLQGLFHRLRALIALNCSIIFVTDGSIPGIKLSTYRRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           SEV +ND NSQQI SLKRNKGSEFSLMIKEAKALGLALGIPCLDG+EE EAQCALLNSE 
Sbjct: 95  SEVAQNDANSQQIPSLKRNKGSEFSLMIKEAKALGLALGIPCLDGIEEAEAQCALLNSES 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSDVFLFGARTVYRDICLG+     C    ++  +L       + LALLLGS
Sbjct: 155 LCDGCFTSDSDVFLFGARTVYRDICLGDGGHVVCYEMADIERQLGFGRNSMVTLALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYIHK 240
           DY+QGVYG+GRESA QIVK VGD  VLQKIASEGLSFT KGKNSKK GL+ SGDGQ +HK
Sbjct: 215 DYSQGVYGLGRESACQIVKAVGDGAVLQKIASEGLSFTKKGKNSKKQGLSKSGDGQCLHK 274

Query: 241 PLFIF------------------LYRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                                  + RVL+QH FQR KLQQ CAE F WPPEKTDEYILPK
Sbjct: 275 CDHFLKVIDAYLKPKCHSADSDAVTRVLVQHPFQRIKLQQICAELFTWPPEKTDEYILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAE+DLRRFANLRSNTS LGFNIPLQE+P +CPVSGIVKHRKVQG++C+EVSWK+L+GL 
Sbjct: 335 IAERDLRRFANLRSNTSALGFNIPLQEIPANCPVSGIVKHRKVQGSDCFEVSWKDLNGLD 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
           SS+VPADLLQSACPEMIVEFEEQ+AQG K N+ +TKTKKSEA VAEID+R +TLLL+IES
Sbjct: 395 SSIVPADLLQSACPEMIVEFEEQKAQGMKQNRRKTKTKKSEAAVAEIDKRLQTLLLQIES 454

Query: 421 ESRAVR--NLSQAPIVSENSNSATGLDDL-QEIILDVEPTIVDHAHGTENVEVINLLSPS 480
           ESRAV   +LS  PIVSE+S SATG+D++ QE  LDVEPTI+D    TE +EVI+LLSPS
Sbjct: 455 ESRAVHSLSLSHTPIVSESSTSATGVDEMNQEDFLDVEPTIIDRTRSTERIEVIDLLSPS 514

Query: 481 PAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           PAIQT KVSKF+Q N EKIDVIDLSD E+D+SPEHERKARELR FL SL+ K
Sbjct: 515 PAIQTSKVSKFQQENSEKIDVIDLSDAESDRSPEHERKARELRAFLVSLRSK 566

[Close]

BLAST of Carg22095 vs. ExPASy TrEMBL
Match: A0A0A0L8N4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G182000 PE=4 SV=1)

HSP 1 Score: 690.6 bits (1781), Expect = 4.7e-195
Identity = 371/532 (69.74%), Postives = 415/532 (78.01%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           +V+L +VSKSH   N K YLKGLF+RLR LIALNCSLIFVTDGSIPGIKLSTYRRRLNNG
Sbjct: 35  IVELSSVSKSHCHSNSKFYLKGLFYRLRTLIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           +EV + D N QQI SLKRNKGSEFSLMIKEA+ALGLALGIPCLD +EE EAQCALLNSEL
Sbjct: 95  NEVAQTDANPQQICSLKRNKGSEFSLMIKEAQALGLALGIPCLDAVEEAEAQCALLNSEL 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLLGS 180
           LCDGCFTSDSD FLFGARTVYRDICLG+     C    ++   L       I  ALLLGS
Sbjct: 155 LCDGCFTSDSDAFLFGARTVYRDICLGDSGHVVCYEMDDIETRLGFGRNSMITFALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYI-- 240
           DY+QGVYGMGRESA Q+VK VGD  VLQKI SEGL+   KG+NSKK GL  SG G Y+  
Sbjct: 215 DYSQGVYGMGRESACQLVKAVGDGAVLQKITSEGLALAKKGENSKKQGLPKSGHGHYMLK 274

Query: 241 ----------------HKPLFIFLYRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYILPK 300
                           H      + RVL QH FQ  KLQQ CAEFF W PEKTDE ILPK
Sbjct: 275 CDEFSEVIDAYLKPKCHSADSEAVTRVLDQHPFQHVKLQQICAEFFEWSPEKTDENILPK 334

Query: 301 IAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDGLS 360
           IAE+DLRRFAN+RS TS+LGFNIPLQE+PVSCP+SGIVKHRKVQGNECYEVSWKN+ GL+
Sbjct: 335 IAERDLRRFANIRSRTSELGFNIPLQEIPVSCPISGIVKHRKVQGNECYEVSWKNI-GLN 394

Query: 361 SSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEIES 420
            SVV ADLLQ ACPEMI+EFEE+RA G+K NK +TKTKKS A +AEID+R +TLLLEIES
Sbjct: 395 LSVVSADLLQRACPEMIIEFEEKRADGKKQNKGKTKTKKSAAVMAEIDKRLKTLLLEIES 454

Query: 421 ESRAVRNLSQAPIVSENSNSATGLDDL-QEIILDVEPTIVDHAH--GTENVEVINLLSPS 480
           ESR V N+S  PI  ++  S TG+ +L QE+ L++EP IVDHA    T+ +EVI+LLSPS
Sbjct: 455 ESRVVHNISHVPI--DSKTSGTGVHELNQEVFLNIEPIIVDHARSCSTKRIEVIDLLSPS 514

Query: 481 PAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           PAIQTR VSKF+Q   +KIDVIDLSDTE DQSPEHERKARELR FLASLKGK
Sbjct: 515 PAIQTRNVSKFQQKTSQKIDVIDLSDTEIDQSPEHERKARELRSFLASLKGK 563

[Close]

BLAST of Carg22095 vs. ExPASy TrEMBL
Match: A0A1S3AYA2 (flap endonuclease GEN-like 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484071 PE=4 SV=1)

HSP 1 Score: 689.5 bits (1778), Expect = 1.0e-194
Identity = 373/534 (69.85%), Postives = 419/534 (78.46%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           +VQL +VSKSH   N K YL+GLF+RLR LIALNCSLIFVTDGSIPGIK+STYRRRLNNG
Sbjct: 35  IVQLSSVSKSHCHSNSKFYLQGLFYRLRTLIALNCSLIFVTDGSIPGIKVSTYRRRLNNG 94

Query: 61  SE--VVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNS 120
           +E  V + D N QQI SLKRN GSEFSLMIKEA+ALGLALGIPCLD +EE EAQCALLNS
Sbjct: 95  NEKQVAQTDANPQQICSLKRNMGSEFSLMIKEAQALGLALGIPCLDAVEEAEAQCALLNS 154

Query: 121 ELLCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVEL-------IALALLL 180
           ELLCDGCFTSDSD FLFGARTVYRDICLG+     C    ++   L       I  ALLL
Sbjct: 155 ELLCDGCFTSDSDAFLFGARTVYRDICLGDSGHVVCYELDDIKTRLGFGRNSMITFALLL 214

Query: 181 GSDYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKKPGLASSGDGQYI 240
           GSDY+QGVYGMGRESA Q+VK VGD  VLQKI SEGL+   KGKNSKK  L  SG+GQYI
Sbjct: 215 GSDYSQGVYGMGRESACQLVKAVGDVAVLQKITSEGLALAKKGKNSKKQELPKSGNGQYI 274

Query: 241 ------------------HKPLFIFLYRVLIQHSFQRAKLQQFCAEFFAWPPEKTDEYIL 300
                             H      + RVL+QH FQR KLQQ CAEFF WPP+KTDE IL
Sbjct: 275 LKCDEFSEVIDAYLKPKCHSADSEAVSRVLVQHPFQRVKLQQICAEFFGWPPDKTDEKIL 334

Query: 301 PKIAEKDLRRFANLRSNTSDLGFNIPLQEVPVSCPVSGIVKHRKVQGNECYEVSWKNLDG 360
           P IAE+DLRRFAN+RS TS+LGFNIPLQE+PVSCPVSGIVKHRKVQGNECYEVSWKN+DG
Sbjct: 335 PWIAERDLRRFANIRSRTSELGFNIPLQEIPVSCPVSGIVKHRKVQGNECYEVSWKNIDG 394

Query: 361 LSSSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVAEIDERFRTLLLEI 420
           L  SVV ADLLQSACPEMIVEFEE+RA+G+K NK +TKTKKS A +AEID+R +TLLLEI
Sbjct: 395 L-ISVVSADLLQSACPEMIVEFEEKRAEGKKQNKGKTKTKKSAAAMAEIDKRLQTLLLEI 454

Query: 421 ESESRAVRNLSQAPIVSENSNSATGLDDL-QEIILDVEPTIVDHAH--GTENVEVINLLS 480
           ESESRAV N+S  PI  ++  S TG+ +L QE+ L++EP IVDHA    T+ +EVI+LLS
Sbjct: 455 ESESRAVHNISHLPI--DSKTSGTGVHELNQEVFLNIEPIIVDHARSCSTKRIEVIDLLS 514

Query: 481 PSPAIQTRKVSKFRQGNCEKIDVIDLSDTENDQSPEHERKARELRLFLASLKGK 500
           PSPAI T +VSKF+Q   +KIDVIDLSDTE DQSPEHERKARELR F+ASLKGK
Sbjct: 515 PSPAIHTHRVSKFQQKTSQKIDVIDLSDTEIDQSPEHERKARELRSFIASLKGK 565

[Close]

BLAST of Carg22095 vs. TAIR 10
Match: AT3G48900.2 (single-stranded DNA endonuclease family protein )

HSP 1 Score: 426.8 bits (1096), Expect = 2.4e-119
Identity = 252/564 (44.68%), Postives = 352/564 (62.41%), Query Frame = 0


Query: 1   MVQLQNVSKSHSCLNQKIYLKGLFHRLRALIALNCSLIFVTDGSIPGIKLSTYRRRLNNG 60
           MV+L  V+KS+    +K+YL+G FHRLRALIALNCS+I V+DG+IPGIK+ TY+RRL   
Sbjct: 35  MVELHKVNKSYCATKEKVYLRGFFHRLRALIALNCSIILVSDGAIPGIKVPTYKRRLKAR 94

Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
            E+  +     + +SLKRN GSEFS +IKEAK +   LGI CLDG+EE EAQCALLNSE 
Sbjct: 95  FEIADDGVEPSKETSLKRNMGSEFSCIIKEAKVIASTLGILCLDGIEEAEAQCALLNSES 154

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVE-------LIALALLLGS 180
           LCD CF+ DSD+FLFGA+TVYR+ICLGE     C    ++  +       LIALALLLGS
Sbjct: 155 LCDACFSFDSDIFLFGAKTVYREICLGEGGYVVCYEMDDIKKKLGLGRNSLIALALLLGS 214

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKK-------------P 240
           DY+QGV G+ +E A ++V+++GD+ +L+K+ASEGLSF  K + SKK             P
Sbjct: 215 DYSQGVRGLRQEKACELVRSIGDNVILEKVASEGLSFAEKPRKSKKQVRPSVCSKKGTLP 274

Query: 241 GLASSGDG------QYIHKPLFIFL------------YRVLIQHSFQRAKLQQFCAEFFA 300
            +  +G+       + I + +  F+             R L + SFQR KLQ+ C +FF 
Sbjct: 275 LVVINGNNRDPERLEEIKQVIDAFMNPKCHQADSNTVSRALAEFSFQRTKLQEICHQFFE 334

Query: 301 WPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQ--EVPVSCPVSGIVKHRKVQG 360
           WPPEKTDEYILPK+AE++LRRFANL+S ++++  N+PL   ++P  CPVS I+K RKVQG
Sbjct: 335 WPPEKTDEYILPKVAERNLRRFANLQSRSTEVEVNLPLHKPQMPEKCPVSEIIKTRKVQG 394

Query: 361 NECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVA 420
            EC+EVSW +L+GL SS+VPADL++ ACPE I+EF+E+ A  +K  K + K K++ +   
Sbjct: 395 RECFEVSWNDLEGLESSIVPADLVERACPEKIIEFKEKMAAKKKKPKPKQKQKETSSPTK 454

Query: 421 E---IDERFRTLLLEIESESRAVRN-LSQAPIVSENSNSATGLDDLQEIILDVEPTIVDH 480
               ++       L++ S S   R+ L +A   +E  NS     D   +I   +    ++
Sbjct: 455 SSSLVELSLELQHLDLNSTSLVSRSTLEEAEQENEQQNSKK--HDYLRLIDSPDRENCNN 514

Query: 481 AHGTEN--------------VEVINLLSPSPAIQTRKVSKFRQ----GNCEKIDVIDLSD 498
           A    +               EVI+L+SP P  ++R VS+  Q     + +   VI+LSD
Sbjct: 515 AWSNRDRLGVGMSSFPLYPETEVIDLISPCPEARSRSVSRSYQEQKSHDHQLETVIELSD 574

[Close]

BLAST of Carg22095 vs. TAIR 10
Match: AT3G48900.1 (single-stranded DNA endonuclease family protein )

HSP 1 Score: 352.1 bits (902), Expect = 7.6e-97
Identity = 215/504 (42.66%), Postives = 305/504 (60.52%), Query Frame = 0


Query: 61  SEVVKNDTNSQQISSLKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSEL 120
           S++  +     + +SLKRN GSEFS +IKEAK +   LGI CLDG+EE EAQCALLNSE 
Sbjct: 31  SQIADDGVEPSKETSLKRNMGSEFSCIIKEAKVIASTLGILCLDGIEEAEAQCALLNSES 90

Query: 121 LCDGCFTSDSDVFLFGARTVYRDICLGE-----CPRACEVSVE-------LIALALLLGS 180
           LCD CF+ DSD+FLFGA+TVYR+ICLGE     C    ++  +       LIALALLLGS
Sbjct: 91  LCDACFSFDSDIFLFGAKTVYREICLGEGGYVVCYEMDDIKKKLGLGRNSLIALALLLGS 150

Query: 181 DYTQGVYGMGRESARQIVKTVGDSTVLQKIASEGLSFTMKGKNSKK-------------P 240
           DY+QGV G+ +E A ++V+++GD+ +L+K+ASEGLSF  K + SKK             P
Sbjct: 151 DYSQGVRGLRQEKACELVRSIGDNVILEKVASEGLSFAEKPRKSKKQVRPSVCSKKGTLP 210

Query: 241 GLASSGDG------QYIHKPLFIFL------------YRVLIQHSFQRAKLQQFCAEFFA 300
            +  +G+       + I + +  F+             R L + SFQR KLQ+ C +FF 
Sbjct: 211 LVVINGNNRDPERLEEIKQVIDAFMNPKCHQADSNTVSRALAEFSFQRTKLQEICHQFFE 270

Query: 301 WPPEKTDEYILPKIAEKDLRRFANLRSNTSDLGFNIPLQ--EVPVSCPVSGIVKHRKVQG 360
           WPPEKTDEYILPK+AE++LRRFANL+S ++++  N+PL   ++P  CPVS I+K RKVQG
Sbjct: 271 WPPEKTDEYILPKVAERNLRRFANLQSRSTEVEVNLPLHKPQMPEKCPVSEIIKTRKVQG 330

Query: 361 NECYEVSWKNLDGLSSSVVPADLLQSACPEMIVEFEEQRAQGRKPNKCRTKTKKSEATVA 420
            EC+EVSW +L+GL SS+VPADL++ ACPE I+EF+E+ A  +K  K + K K++ +   
Sbjct: 331 RECFEVSWNDLEGLESSIVPADLVERACPEKIIEFKEKMAAKKKKPKPKQKQKETSSPTK 390

Query: 421 E---IDERFRTLLLEIESESRAVRN-LSQAPIVSENSNSATGLDDLQEIILDVEPTIVDH 480
               ++       L++ S S   R+ L +A   +E  NS     D   +I   +    ++
Sbjct: 391 SSSLVELSLELQHLDLNSTSLVSRSTLEEAEQENEQQNSKK--HDYLRLIDSPDRENCNN 450

Query: 481 AHGTEN--------------VEVINLLSPSPAIQTRKVSKFRQ----GNCEKIDVIDLSD 498
           A    +               EVI+L+SP P  ++R VS+  Q     + +   VI+LSD
Sbjct: 451 AWSNRDRLGVGMSSFPLYPETEVIDLISPCPEARSRSVSRSYQEQKSHDHQLETVIELSD 510

[Close]

BLAST of Carg22095 vs. TAIR 10
Match: AT3G28030.1 (5'-3' exonuclease family protein )

HSP 1 Score: 84.3 bits (207), Expect = 2.9e-16
Identity = 58/154 (37.66%), Postives = 81/154 (52.60%), Query Frame = 0


Query: 76   LKRNKGSEFSLMIKEAKALGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDVFLF 135
            L+RN  S  S M  E + L    GIP +    E EAQCA +    L DG  T DSDVFLF
Sbjct: 914  LERNAESVSSEMFAECQELLQIFGIPYIIAPMEAEAQCAFMEQSNLVDGIVTDDSDVFLF 973

Query: 136  GARTVYRDI----------CLGECPRACEVSVE-LIALALLLGSDYTQGVYGMGRESARQ 195
            GAR+VY++I           + +  +   +S + +I +A+LLGSDYT+G+ G+G  +A +
Sbjct: 974  GARSVYKNIFDDRKYVETYFMKDIEKELGLSRDKIIRMAMLLGSDYTEGISGIGIVNAIE 1033

Query: 196  IVKTVGDSTVLQKIAS--EGLSFTMKGKNSKKPG 217
            +V    +   LQK     E    T+ GK   K G
Sbjct: 1034 VVTAFPEEDGLQKFREWVESPDPTILGKTDAKTG 1067

[Close]

BLAST of Carg22095 vs. TAIR 10
Match: AT1G01880.2 (5'-3' exonuclease family protein )

HSP 1 Score: 82.0 bits (201), Expect = 1.5e-15
Identity = 71/202 (35.15%), Postives = 98/202 (48.51%), Query Frame = 0


Query: 38  IFVTDGSIPGIKLSTYRRRLNNGSEVVKNDTNSQQI----SSLKRNKGSEFSLMIKEAKA 97
           +FV DG+   +K      R    S +   DT +  +     S++RNK   FS  ++E + 
Sbjct: 71  VFVVDGTPSPLKSQARISRFFRSSGI---DTCNLPVIKDGVSVERNK--LFSEWVRECEL 130

Query: 98  LGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDVFLFGARTVYRDICLG-----E 157
           L L LGIP L    E EA CA LNS+   D C T DSD FLFGA  V +DI        E
Sbjct: 131 LEL-LGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNSREPFE 190

Query: 158 CPRACEVS-------VELIALALLLGSDY-TQGVYGMGRESARQIVKTVGDSTVLQKIAS 217
           C     +          LIA++LL+G+DY + GV G+G + A +IV+   +  VL+++  
Sbjct: 191 CYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVREFSEDQVLERLQD 250

Query: 218 EGLSFTMKGKNSKKPGLASSGD 223
            G      G     PG   SGD
Sbjct: 251 IG-----NGLQPAVPGGIKSGD 261

[Close]

BLAST of Carg22095 vs. TAIR 10
Match: AT1G01880.1 (5'-3' exonuclease family protein )

HSP 1 Score: 81.6 bits (200), Expect = 1.9e-15
Identity = 70/202 (34.65%), Postives = 96/202 (47.52%), Query Frame = 0


Query: 38  IFVTDGSIPGIKLSTYRRRLNNGSEVVKNDTNSQQI----SSLKRNKGSEFSLMIKEAKA 97
           +FV DG+   +K      R    S +   DT +  +     S++RNK   FS  ++E   
Sbjct: 71  VFVVDGTPSPLKSQARISRFFRSSGI---DTCNLPVIKDGVSVERNK--LFSEWVRECVE 130

Query: 98  LGLALGIPCLDGLEEGEAQCALLNSELLCDGCFTSDSDVFLFGARTVYRDICLG-----E 157
           L   LGIP L    E EA CA LNS+   D C T DSD FLFGA  V +DI        E
Sbjct: 131 LLELLGIPVLKANGEAEALCAQLNSQGFVDACITPDSDAFLFGAMCVIKDIKPNSREPFE 190

Query: 158 CPRACEVS-------VELIALALLLGSDY-TQGVYGMGRESARQIVKTVGDSTVLQKIAS 217
           C     +          LIA++LL+G+DY + GV G+G + A +IV+   +  VL+++  
Sbjct: 191 CYHMSHIESGLGLKRKHLIAISLLVGNDYDSGGVLGIGVDKALRIVREFSEDQVLERLQD 250

Query: 218 EGLSFTMKGKNSKKPGLASSGD 223
            G      G     PG   SGD
Sbjct: 251 IG-----NGLQPAVPGGIKSGD 262

The following BLAST results are available for this feature:

BLAST of Carg22095 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013227.1	6.1e-282	100.00	Flap endonuclease GEN-like 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6574173.1	1.8e-262	91.12	Flap endonuclease GEN-like 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022944807.1	4.5e-261	90.55	flap endonuclease GEN-like 2 [Cucurbita moschata]	[more]
XP_023541410.1	4.3e-251	88.09	flap endonuclease GEN-like 2 [Cucurbita pepo subsp. pepo]	[more]
XP_022968219.1	2.8e-250	86.96	flap endonuclease GEN-like 2 [Cucurbita maxima]	[more]

BLAST of Carg22095 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M2Z3	3.4e-118	44.68	Single-strand DNA endonuclease 1 OS=Arabidopsis thaliana OX=3702 GN=SEND1 PE=1 S...	[more]
Q8W5R1	8.7e-114	47.28	Single-strand DNA endonuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SEN...	[more]
Q17RS7	4.4e-17	31.79	Flap endonuclease GEN homolog 1 OS=Homo sapiens OX=9606 GN=GEN1 PE=1 SV=2	[more]
Q8BMI4	7.5e-17	30.10	Flap endonuclease GEN homolog 1 OS=Mus musculus OX=10090 GN=Gen1 PE=1 SV=2	[more]
P61942	6.4e-16	31.68	Flap endonuclease 1 OS=Nanoarchaeum equitans (strain Kin4-M) OX=228908 GN=fen PE...	[more]

BLAST of Carg22095 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FZ58	2.2e-261	90.55	flap endonuclease GEN-like 2 OS=Cucurbita moschata OX=3662 GN=LOC111449235 PE=4 ...	[more]
A0A6J1HZ11	1.3e-250	86.96	flap endonuclease GEN-like 2 OS=Cucurbita maxima OX=3661 GN=LOC111467522 PE=4 SV...	[more]
A0A6J1CIZ7	2.4e-215	74.81	flap endonuclease GEN-like 2 OS=Momordica charantia OX=3673 GN=LOC111011427 PE=4...	[more]
A0A0A0L8N4	4.7e-195	69.74	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G182000 PE=4 SV=1	[more]
A0A1S3AYA2	1.0e-194	69.85	flap endonuclease GEN-like 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103484071 ...	[more]

BLAST of Carg22095 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48900.2	2.4e-119	44.68	single-stranded DNA endonuclease family protein	[more]
AT3G48900.1	7.6e-97	42.66	single-stranded DNA endonuclease family protein	[more]
AT3G28030.1	2.9e-16	37.66	5'-3' exonuclease family protein	[more]
AT1G01880.2	1.5e-15	35.15	5'-3' exonuclease family protein	[more]
AT1G01880.1	1.9e-15	34.65	5'-3' exonuclease family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006084	XPG/Rad2 endonuclease	PRINTS	PR00853	XPGRADSUPER	coord: 95..112 score: 35.42 coord: 37..56 score: 34.84 coord: 116..136 score: 37.35
IPR006084	XPG/Rad2 endonuclease	PANTHER	PTHR11081	FLAP ENDONUCLEASE FAMILY MEMBER	coord: 1..405
IPR006086	XPG-I domain	SMART	SM00484	xpgineu	coord: 96..166 e-value: 2.9E-6 score: 36.8
IPR006086	XPG-I domain	PFAM	PF00867	XPG_I	coord: 98..162 e-value: 6.9E-13 score: 48.8
None	No IPR available	GENE3D	3.40.50.1010		coord: 1..159 e-value: 2.3E-25 score: 91.2
None	No IPR available	GENE3D	2.40.50.40		coord: 302..369 e-value: 3.0E-7 score: 32.0
None	No IPR available	GENE3D	1.10.150.20		coord: 160..212 e-value: 2.3E-7 score: 32.7
None	No IPR available	PANTHER	PTHR11081:SF54	FLAP ENDONUCLEASE GEN HOMOLOG 1	coord: 1..405
None	No IPR available	CDD	cd09900	H3TH_XPG-like	coord: 158..200 e-value: 8.18481E-9 score: 49.7912
None	No IPR available	CDD	cd00024	CD_CSD	coord: 304..352 e-value: 1.51478E-5 score: 40.1529
IPR000953	Chromo/chromo shadow domain	PROSITE	PS50013	CHROMO_2	coord: 302..362 score: 8.937501
IPR029060	PIN-like domain superfamily	SUPERFAMILY	88723	PIN domain-like	coord: 15..169
IPR036279	5'-3' exonuclease, C-terminal domain superfamily	SUPERFAMILY	47807	5' to 3' exonuclease, C-terminal subdomain	coord: 158..283
IPR016197	Chromo-like domain superfamily	SUPERFAMILY	54160	Chromo domain-like	coord: 304..360

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg22095-RA	Carg22095-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
molecular_function	GO:0017108	5'-flap endonuclease activity
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0004518	nuclease activity