Carg21960 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21960
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionreceptor-like serine/threonine-protein kinase ALE2
LocationCarg_Chr11: 3122182 .. 3126204 (-)
RNA-Seq ExpressionCarg21960
SyntenyCarg21960
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
AAGCAAATAAGAACAGGGTCTCATTCATTCTTTAGAGATTTTGCGTCAGTTTCTTTCAGAGAAACGCTTCTTTTGGTCTCGTTCATTGAAAACGGATGCTTCGCAGAGCTCTGGGTTTTTCAACACATCGCCCTGTTTGTGGGTCTTCTTCTTCCAACCTCCTCCTCATTGTTCTTGCTTCTTCAACTTTTAAATCCCTAACTCATTCCTTTGAATCTGTTTCTTCTGTTTCTTCGATGTTGAATAAATTTATCGTCGTTTGAGTTTGTATGAGATGGAGGTTCTGGTTCCAACTATTCTTCACCTACTGATTCTTTTGGTCACTGTTCATGCTTCCGAGCTTCGGGACATTGCAGGTACATTTCTTCCTTATTCGCATTTGGATTTCACAATCTGAGTAGTTGACATTTGAATGAACTTGAGTTGATCTAGCGGAATTGCTGTTCTTGTTTGAAGTTTAATCCAATTCTTAGTTCATTCACAACACCGAAGATTTTAGTGAAAATATACAGAATCATGGAGAAATAACAATGTGATGTGTCTGGATCTAGGTTTTTGAACTTCTGGAGAATCATGAAGTTCATAGTTCGACAAATTTGAACTTCATTCATGGAATATTTTCATGGTTGTTTGATCCTATCTACTTGAAACTCTTTGTTTGATTGGTTAAATTTGATGTTCCAGAGCCTTATATCTCTCCAGCAGTAGCTCTTTCCCCTAATAATGCATTACCACCTCTGAATCCTTCCCCGGAAGAAGGTATCCTGTTAAATTGTTCTTGAACAAACAAACTCTCTTCCTGTTGCTTATTTTCTAGTGTCTTTTATGAAGCTCCTTCCCCTCACAATGACATGGGACACTCTCATGATGCTGAACAGCCAATTCTTCCACCACAAGAAGCTCATGATGTTGAACAGCCAAATGAAGGTATATGTCCACTTCCACTTAAGTTTCTTCTCATCTAGATTACAGACAAATGAAGTTAGTATTCAAGAACATGTTCCACTGTCTTTTCTAGATTGTTCGGCTACCCTATGCACCGATCCGTATACAAATACTCCCCCGGGATCCCCCTGTGGTTGTGTTTGGCCCATACAAGTTCGACTTCGTTTCAGTGTTCCCCTCTATACCTTCTTCCCCCTAGTTTCAGAGCTGGCTGCTGAAATTGCTGCTGGGGTATATGTAAAGCGAAGTCAAGTTCGTGTTATTGAGGCCAATGCAGCTTATCAGCAACCAGATAAGACTGTTGCCCTTATTGACTTAGTCCCACTTGGGGAAAAATTTGACAATACTACAGCCTTTTTGACTTACCAAAGGTTTTGGCATAAAAAAGTTTCTATCAAAGCTTACTATTTCGGGAACTATGAAGTGTTATACGTTCGATATCCAGGTTTGCCTCGATCGCCCCCTCCTGGAGTTTCAGGGCTAGATAATGAGCCTCATTTGACTAATACTAACAACGCGAGGACAGTAAAACCCGTCGGTGTCGATGTGCACAACAGACAGCCAAAAGATAGAATAAGTGATGGTATGATCGCTATCATCACCCTATCAACTACTGTTGCAGTGGGCTCATGTCTTGTTACTGCTTGGATTTTGATCTTTACACACAATGATGCAAAACCAAATCCACATGTTTCGTTATCCGCCCTCAATAAGCCATCAGGTAACATGGATTGCCAACTTATGCAGCCTCCAACGGTGGTTCTATCCTCTTGTAGCATTTTTAAGTTTATACAATCTTGCAGGTGGTGGTGGATTAGTTATGGCAACCATGCCTAGTTCTGCCTCTTTATCCTTTGGATATAGCATTGCTTCATATTCAGGATCAGCCAAGATCTTCAGTGCCTCTGACATAGAGAGAACCACAAATAATTTTGATCCTTCAAGAATACTGGGGGAGGGTGGGTTTGGACGTGTTTACAGTGGCACGCTCGAAGATGGTATCGAAGTAGCAATCAAAGTTCTCAAGAGAGATGATCATCAGGGCGGTCGAGAATTCTTGGCTGAAGTTGAAATGCTTAGCCGCCTCCACCACAGAAATTTGGTCAAGTTGATTGGAATATGTACTGAGGAGCATAACTGTTCCCTGGTGTATGAACTTGTTCCAAATGGCAGTGTTGAATCTCATTTACATGGTAAGAAAGTGATGTTCATGCGTTTCCTTTGTAGTATAAGCAGAGCATGGTTTCATTTTTCTCTATACTTTTCTGTCAGGTATAGATAAGGAAACTGGCCTACTTGATTGGGGTGCCAGGATGAAGATAGCGCTGGGCGCTGCTCGGGGTTTATCCTATCTACATGAGGATTCGAGTCCTAGAGTCATACATCGAGATTTCAAATCGAGTAATATCTTACTAGAACATGATTTTACACCAAAAGTTTCCGACTTCGGGTTGGCTCGAACTGCAACAGATGAGGAAAGCAGACACATAACTACACATGTCATGGGAACTTTCGGGTATAGCATTCCTTCCTTTTGAACGTTATTAGAAGTTCTTTTGGATTTCACTCTGCCAAATATAGTAAAGAGCCAAGTTGAGTCTGTGGTAGTTAATTTACTTTTCACATTCTTAAGCATGGTTACTCTCAACGTTTTTAAAACGCGTCTGCTACGGAGAGGTTTACATACCCTTATAAAAAAAATAGTTTTGTTCCCCTCTCTAACCAATGTGAGATCTCACAATTCACCCCCTTTAAGGCCCAGCGTCTTCGCTGGCACTCGTTCCCCTCTCCAATCAATGTTGTTCCTCTCGCCAATCGATGTGGGATCTCACAAGCCACCCTCCTTTGGGGCTCAACGTCCTTGCTGACACACTGCCTAGTGTTTGGCTCTAATACCATTTATAACAGCCCAAGGCTACCGTCAGCAGGTATTGTCCTTTTTGGACTTTTTCTCTCAGATTTTCTCTCGAGGTTTTTAAAATGCATCTACTAGGGAGAGGTTTTCACACTCTTATAAAGAATGTTTTGTTCCCCTCTCCAACCGATGTGGGATCTCATAGTCTCAACTTGAGCCACTCTAAAAAGCTACAAAATAGAATTTGGAACATGTTATTACAAGTGCAATTAAGTTAAACTAGGCTAAGATTTTGCTTGAATCATGAAAATCTTGAATAAATGTTGAAAACTTGAATTATAGCAAACTTGATTACATAGAGTAACGTTTATTCTTGATTTCAACTTGAGGAGTAGAAACTCTGACATGTCCTCACCAACGATGTACTTCTGGAAACAAACCCCAAATATGTATAATAATAATGAGATTGGTATGGATTTGTTTTCTCTCATTTTTGTATTCCATGCCTTATTACTTGACTTATTGTCTCTCCTTGTGTACTCAGGTACGTGGCTCCAGAGTATGCAATGACTGGCCATCTACTTGTGAAGAGTGATGTTTATAGTTACGGAGTTGTTCTACTTGAACTATTAACAGGTAGGAAACCAATTGATATGTCTCAACCACCCGGTGAAGAAAATCTCGTTGCTTGGGCTCGTCCACTCCTCACCAGCAAAGAAGGTCTAGATGTTATAATTGATAAATCACTAGATTCCAATGTTCCTTTTGAAAACATTGCCAAAGTTGCAGCAATTGCTTCAATGTGTGTGCAACCAGAGGTGTCACACCGACCTTTCATGAGTGAGGTCGTTCAAGCATTGAAACTAGTTTGCAACCAATGTGATGAGGTTCAAGAAACAAGCTCGCAAACTAATTCGAGCTCAAAGAATTCATTTGTGGATATGAATGATGAAGCTTCGAGTTCAAAACTCATGCTAGACTCTTTTCATAGCGAATATTTAGTACCTAACTCAATTTCAGGTCTTGATGGTATCGATACACGTCTCTCGATTTCAGACTTGTTAAATACTTCCATCAATATGGGAATGCAAGAATCAAGATCACTCAGAAGATGCTTCAGTTCCGCTCCTCTTAGAATCGAGAAAAGCAAAAGAAATTTTTGGCAAAGAATGAGAAGTAGCATGAGTGAACATGGAATGCCTAGATTATCCCATACTCCCTAA

mRNA sequence

AAGCAAATAAGAACAGGGTCTCATTCATTCTTTAGAGATTTTGCGTCAGTTTCTTTCAGAGAAACGCTTCTTTTGGTCTCGTTCATTGAAAACGGATGCTTCGCAGAGCTCTGGGTTTTTCAACACATCGCCCTGTTTGTGGGTCTTCTTCTTCCAACCTCCTCCTCATTGTTCTTGCTTCTTCAACTTTTAAATCCCTAACTCATTCCTTTGAATCTGTTTCTTCTGTTTCTTCGATGTTGAATAAATTTATCGTCGTTTGAGTTTGTATGAGATGGAGGTTCTGGTTCCAACTATTCTTCACCTACTGATTCTTTTGGTCACTGTTCATGCTTCCGAGCTTCGGGACATTGCAGAGCCTTATATCTCTCCAGCAGTAGCTCTTTCCCCTAATAATGCATTACCACCTCTGAATCCTTCCCCGGAAGAAGCTCCTTCCCCTCACAATGACATGGGACACTCTCATGATGCTGAACAGCCAATTCTTCCACCACAAGAAGCTCATGATGTTGAACAGCCAAATGAAGATTGTTCGGCTACCCTATGCACCGATCCGTATACAAATACTCCCCCGGGATCCCCCTGTGGTTGTGTTTGGCCCATACAAGTTCGACTTCGTTTCAGTGTTCCCCTCTATACCTTCTTCCCCCTAGTTTCAGAGCTGGCTGCTGAAATTGCTGCTGGGGTATATGTAAAGCGAAGTCAAGTTCGTGTTATTGAGGCCAATGCAGCTTATCAGCAACCAGATAAGACTGTTGCCCTTATTGACTTAGTCCCACTTGGGGAAAAATTTGACAATACTACAGCCTTTTTGACTTACCAAAGGTTTTGGCATAAAAAAGTTTCTATCAAAGCTTACTATTTCGGGAACTATGAAGTGTTATACGTTCGATATCCAGGTTTGCCTCGATCGCCCCCTCCTGGAGTTTCAGGGCTAGATAATGAGCCTCATTTGACTAATACTAACAACGCGAGGACAGTAAAACCCGTCGGTGTCGATGTGCACAACAGACAGCCAAAAGATAGAATAAGTGATGGTATGATCGCTATCATCACCCTATCAACTACTGTTGCAGTGGGCTCATGTCTTGTTACTGCTTGGATTTTGATCTTTACACACAATGATGCAAAACCAAATCCACATGTTTCGTTATCCGCCCTCAATAAGCCATCAGGTGGTGGTGGATTAGTTATGGCAACCATGCCTAGTTCTGCCTCTTTATCCTTTGGATATAGCATTGCTTCATATTCAGGATCAGCCAAGATCTTCAGTGCCTCTGACATAGAGAGAACCACAAATAATTTTGATCCTTCAAGAATACTGGGGGAGGGTGGGTTTGGACGTGTTTACAGTGGCACGCTCGAAGATGGTATCGAAGTAGCAATCAAAGTTCTCAAGAGAGATGATCATCAGGGCGGTCGAGAATTCTTGGCTGAAGTTGAAATGCTTAGCCGCCTCCACCACAGAAATTTGGTCAAGTTGATTGGAATATGTACTGAGGAGCATAACTGTTCCCTGGTGTATGAACTTGTTCCAAATGGCAGTGTTGAATCTCATTTACATGGTATAGATAAGGAAACTGGCCTACTTGATTGGGGTGCCAGGATGAAGATAGCGCTGGGCGCTGCTCGGGGTTTATCCTATCTACATGAGGATTCGAGTCCTAGAGTCATACATCGAGATTTCAAATCGAGTAATATCTTACTAGAACATGATTTTACACCAAAAGTTTCCGACTTCGGGTTGGCTCGAACTGCAACAGATGAGGAAAGCAGACACATAACTACACATGTCATGGGAACTTTCGGGTACGTGGCTCCAGAGTATGCAATGACTGGCCATCTACTTGTGAAGAGTGATGTTTATAGTTACGGAGTTGTTCTACTTGAACTATTAACAGGTAGGAAACCAATTGATATGTCTCAACCACCCGGTGAAGAAAATCTCGTTGCTTGGGCTCGTCCACTCCTCACCAGCAAAGAAGGTCTAGATGTTATAATTGATAAATCACTAGATTCCAATGTTCCTTTTGAAAACATTGCCAAAGTTGCAGCAATTGCTTCAATGTGTGTGCAACCAGAGGTGTCACACCGACCTTTCATGAGTGAGGTCGTTCAAGCATTGAAACTAGTTTGCAACCAATGTGATGAGGTTCAAGAAACAAGCTCGCAAACTAATTCGAGCTCAAAGAATTCATTTGTGGATATGAATGATGAAGCTTCGAGTTCAAAACTCATGCTAGACTCTTTTCATAGCGAATATTTAGTACCTAACTCAATTTCAGGTCTTGATGGTATCGATACACGTCTCTCGATTTCAGACTTGTTAAATACTTCCATCAATATGGGAATGCAAGAATCAAGATCACTCAGAAGATGCTTCAGTTCCGCTCCTCTTAGAATCGAGAAAAGCAAAAGAAATTTTTGGCAAAGAATGAGAAGTAGCATGAGTGAACATGGAATGCCTAGATTATCCCATACTCCCTAA

Coding sequence (CDS)

ATGGAGGTTCTGGTTCCAACTATTCTTCACCTACTGATTCTTTTGGTCACTGTTCATGCTTCCGAGCTTCGGGACATTGCAGAGCCTTATATCTCTCCAGCAGTAGCTCTTTCCCCTAATAATGCATTACCACCTCTGAATCCTTCCCCGGAAGAAGCTCCTTCCCCTCACAATGACATGGGACACTCTCATGATGCTGAACAGCCAATTCTTCCACCACAAGAAGCTCATGATGTTGAACAGCCAAATGAAGATTGTTCGGCTACCCTATGCACCGATCCGTATACAAATACTCCCCCGGGATCCCCCTGTGGTTGTGTTTGGCCCATACAAGTTCGACTTCGTTTCAGTGTTCCCCTCTATACCTTCTTCCCCCTAGTTTCAGAGCTGGCTGCTGAAATTGCTGCTGGGGTATATGTAAAGCGAAGTCAAGTTCGTGTTATTGAGGCCAATGCAGCTTATCAGCAACCAGATAAGACTGTTGCCCTTATTGACTTAGTCCCACTTGGGGAAAAATTTGACAATACTACAGCCTTTTTGACTTACCAAAGGTTTTGGCATAAAAAAGTTTCTATCAAAGCTTACTATTTCGGGAACTATGAAGTGTTATACGTTCGATATCCAGGTTTGCCTCGATCGCCCCCTCCTGGAGTTTCAGGGCTAGATAATGAGCCTCATTTGACTAATACTAACAACGCGAGGACAGTAAAACCCGTCGGTGTCGATGTGCACAACAGACAGCCAAAAGATAGAATAAGTGATGGTATGATCGCTATCATCACCCTATCAACTACTGTTGCAGTGGGCTCATGTCTTGTTACTGCTTGGATTTTGATCTTTACACACAATGATGCAAAACCAAATCCACATGTTTCGTTATCCGCCCTCAATAAGCCATCAGGTGGTGGTGGATTAGTTATGGCAACCATGCCTAGTTCTGCCTCTTTATCCTTTGGATATAGCATTGCTTCATATTCAGGATCAGCCAAGATCTTCAGTGCCTCTGACATAGAGAGAACCACAAATAATTTTGATCCTTCAAGAATACTGGGGGAGGGTGGGTTTGGACGTGTTTACAGTGGCACGCTCGAAGATGGTATCGAAGTAGCAATCAAAGTTCTCAAGAGAGATGATCATCAGGGCGGTCGAGAATTCTTGGCTGAAGTTGAAATGCTTAGCCGCCTCCACCACAGAAATTTGGTCAAGTTGATTGGAATATGTACTGAGGAGCATAACTGTTCCCTGGTGTATGAACTTGTTCCAAATGGCAGTGTTGAATCTCATTTACATGGTATAGATAAGGAAACTGGCCTACTTGATTGGGGTGCCAGGATGAAGATAGCGCTGGGCGCTGCTCGGGGTTTATCCTATCTACATGAGGATTCGAGTCCTAGAGTCATACATCGAGATTTCAAATCGAGTAATATCTTACTAGAACATGATTTTACACCAAAAGTTTCCGACTTCGGGTTGGCTCGAACTGCAACAGATGAGGAAAGCAGACACATAACTACACATGTCATGGGAACTTTCGGGTACGTGGCTCCAGAGTATGCAATGACTGGCCATCTACTTGTGAAGAGTGATGTTTATAGTTACGGAGTTGTTCTACTTGAACTATTAACAGGTAGGAAACCAATTGATATGTCTCAACCACCCGGTGAAGAAAATCTCGTTGCTTGGGCTCGTCCACTCCTCACCAGCAAAGAAGGTCTAGATGTTATAATTGATAAATCACTAGATTCCAATGTTCCTTTTGAAAACATTGCCAAAGTTGCAGCAATTGCTTCAATGTGTGTGCAACCAGAGGTGTCACACCGACCTTTCATGAGTGAGGTCGTTCAAGCATTGAAACTAGTTTGCAACCAATGTGATGAGGTTCAAGAAACAAGCTCGCAAACTAATTCGAGCTCAAAGAATTCATTTGTGGATATGAATGATGAAGCTTCGAGTTCAAAACTCATGCTAGACTCTTTTCATAGCGAATATTTAGTACCTAACTCAATTTCAGGTCTTGATGGTATCGATACACGTCTCTCGATTTCAGACTTGTTAAATACTTCCATCAATATGGGAATGCAAGAATCAAGATCACTCAGAAGATGCTTCAGTTCCGCTCCTCTTAGAATCGAGAAAAGCAAAAGAAATTTTTGGCAAAGAATGAGAAGTAGCATGAGTGAACATGGAATGCCTAGATTATCCCATACTCCCTAA

Protein sequence

MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDMGHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPLYTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFLTYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVGVDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQRMRSSMSEHGMPRLSHTP
Homology
BLAST of Carg21960 vs. NCBI nr
Match: KAG6588041.1 (Receptor-like serine/threonine-protein kinase ALE2, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021937.1 Receptor-like serine/threonine-protein kinase ALE2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1454.5 bits (3764), Expect = 0.0e+00
Identity = 737/737 (100.00%), Postives = 737/737 (100.00%), Query Frame = 0

Query: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60
           MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM
Sbjct: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60

Query: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120
           GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL
Sbjct: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120

Query: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180
           YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL
Sbjct: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180

Query: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240
           TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG
Sbjct: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240

Query: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300
           VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS
Sbjct: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300

Query: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360
           GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS
Sbjct: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360

Query: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420
           GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV
Sbjct: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420

Query: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480
           PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH
Sbjct: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480

Query: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540
           DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL
Sbjct: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540

Query: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600
           LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV
Sbjct: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600

Query: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660
           QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH
Sbjct: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660

Query: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720
           SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR
Sbjct: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720

Query: 721 MRSSMSEHGMPRLSHTP 738
           MRSSMSEHGMPRLSHTP
Sbjct: 721 MRSSMSEHGMPRLSHTP 737

BLAST of Carg21960 vs. NCBI nr
Match: XP_022933312.1 (receptor-like serine/threonine-protein kinase ALE2 [Cucurbita moschata])

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 726/737 (98.51%), Postives = 732/737 (99.32%), Query Frame = 0

Query: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60
           MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLN SPEEAPSPHNDM
Sbjct: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNHSPEEAPSPHNDM 60

Query: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120
           GHSHDA+QPILP QEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL
Sbjct: 61  GHSHDADQPILPSQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120

Query: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180
           YTFFPLVSELAAEIA+GVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL
Sbjct: 121 YTFFPLVSELAAEIASGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180

Query: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240
           TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG
Sbjct: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240

Query: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300
           VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS
Sbjct: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300

Query: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360
           GGGG VMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS
Sbjct: 301 GGGGSVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360

Query: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420
           GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV
Sbjct: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420

Query: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480
           PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH
Sbjct: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480

Query: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540
           DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL
Sbjct: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540

Query: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600
           LTGRKP+DMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV
Sbjct: 541 LTGRKPVDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600

Query: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660
           QPEVSHRPFMSEVVQALKLVCNQCDEV+ETSSQTNSSSKNSFV+MNDEASSSKL+LDSFH
Sbjct: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVRETSSQTNSSSKNSFVNMNDEASSSKLVLDSFH 660

Query: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720
           SEYLVPNSISGLDGIDTRLSISDLLNTSI MGMQESRSLRRCFSSAPLRIEKSKRNFWQR
Sbjct: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSIYMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720

Query: 721 MRSSMSEHGMPRLSHTP 738
           MRSSM EHGMPRLSHTP
Sbjct: 721 MRSSMREHGMPRLSHTP 737

BLAST of Carg21960 vs. NCBI nr
Match: XP_023531637.1 (receptor-like serine/threonine-protein kinase ALE2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1419.8 bits (3674), Expect = 0.0e+00
Identity = 722/736 (98.10%), Postives = 728/736 (98.91%), Query Frame = 0

Query: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60
           MEVLVPTILHLLILLVTVHASELR+IAEPYISPAVALSPNNALP LN SPEEAPSPH DM
Sbjct: 1   MEVLVPTILHLLILLVTVHASELREIAEPYISPAVALSPNNALPLLNHSPEEAPSPHKDM 60

Query: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120
           GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL
Sbjct: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120

Query: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180
           YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL
Sbjct: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180

Query: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240
           TYQRFWHKKVSI AYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG
Sbjct: 181 TYQRFWHKKVSINAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240

Query: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300
           VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS
Sbjct: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300

Query: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360
           G GG VMATMPSS+SLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS
Sbjct: 301 GAGGSVMATMPSSSSLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360

Query: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420
           GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV
Sbjct: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420

Query: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480
           PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH
Sbjct: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480

Query: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540
           DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL
Sbjct: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540

Query: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600
           LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV
Sbjct: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600

Query: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660
           QPEVSHRPFMSEVVQALKLVCNQCDEV+ETSSQTNSSSKNS VDMNDEASSSKL+LDSFH
Sbjct: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVRETSSQTNSSSKNSSVDMNDEASSSKLVLDSFH 660

Query: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720
           SEYLVPN+ISGLDGIDTRLSISDLLNTSI+MGMQESRSLRRCFSSAPLRIEKSKRNFWQR
Sbjct: 661 SEYLVPNAISGLDGIDTRLSISDLLNTSIDMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720

Query: 721 MRSSMSEHGMPRLSHT 737
           MRSSMSEHGM RLSHT
Sbjct: 721 MRSSMSEHGMLRLSHT 736

BLAST of Carg21960 vs. NCBI nr
Match: XP_022966870.1 (receptor-like serine/threonine-protein kinase ALE2 [Cucurbita maxima])

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 709/737 (96.20%), Postives = 722/737 (97.96%), Query Frame = 0

Query: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60
           MEVLVPT+LHLLILLVTVHASELR+IAEPYISPAVALSPNNALPPLN SPEEAPS HNDM
Sbjct: 1   MEVLVPTMLHLLILLVTVHASELREIAEPYISPAVALSPNNALPPLNHSPEEAPSTHNDM 60

Query: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120
           GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL
Sbjct: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120

Query: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180
           YTFFPLVSELAAEIA+GVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL
Sbjct: 121 YTFFPLVSELAAEIASGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180

Query: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240
           TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNN RTVKPVG
Sbjct: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNVRTVKPVG 240

Query: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300
           VDVHNRQPKDRISDGMIAIITLSTTVAVG CLVTAWIL+FTHNDAKPNPHVSLSALNKPS
Sbjct: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGLCLVTAWILLFTHNDAKPNPHVSLSALNKPS 300

Query: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360
           G GG VMAT+PSSASL FGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS
Sbjct: 301 GAGGSVMATIPSSASLFFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360

Query: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420
           GTLEDG EVAIKVLKRDDHQG REFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV
Sbjct: 361 GTLEDGTEVAIKVLKRDDHQGSREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420

Query: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480
           PNGSVESHLHG+DKETGLLDWGARMKIALGAARGLSYLHEDS PRVIHRDFKSSNILLEH
Sbjct: 421 PNGSVESHLHGMDKETGLLDWGARMKIALGAARGLSYLHEDSIPRVIHRDFKSSNILLEH 480

Query: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540
           +FTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL
Sbjct: 481 NFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540

Query: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600
           LTGRKPIDMSQPPGEE+LVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV
Sbjct: 541 LTGRKPIDMSQPPGEESLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600

Query: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660
           QPEVSHRPFM EVVQALKLVCNQCD VQETSSQTNSSSKNS VDMNDEASSSKL+LDSFH
Sbjct: 601 QPEVSHRPFMGEVVQALKLVCNQCDGVQETSSQTNSSSKNSSVDMNDEASSSKLVLDSFH 660

Query: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720
           S+YLVPNSISGLDGIDTRLSISDLLNTSI+MGMQESRSL+RCFSSA LRIEKSKRN+WQR
Sbjct: 661 SKYLVPNSISGLDGIDTRLSISDLLNTSIDMGMQESRSLKRCFSSATLRIEKSKRNYWQR 720

Query: 721 MRSSMSEHGMPRLSHTP 738
           M SSMSEHGMPRLSHTP
Sbjct: 721 MISSMSEHGMPRLSHTP 737

BLAST of Carg21960 vs. NCBI nr
Match: XP_023516974.1 (receptor-like serine/threonine-protein kinase ALE2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 575/747 (76.97%), Postives = 624/747 (83.53%), Query Frame = 0

Query: 19  HASELRDIAEPYISPAVALSPNN-ALPPL----NPSPEEAPSPHNDM-GHSHD------- 78
           H+ E  D A P  S   ++SP++   PPL    +PSP  +PSP+  M GH+         
Sbjct: 195 HSHERGDYAPPMESDPSSISPSHYDRPPLVTHVSPSPSPSPSPYQKMSGHTERHFYPPKA 254

Query: 79  --------AEQPILPP-QEAHDV--EQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRL 138
                   A+ P +PP +  H +    PNEDCSATLCT+PYTNTPPGSPCGCVWP+Q+ L
Sbjct: 255 SPSPLKPWAKSPKMPPLRPIHTLPPPPPNEDCSATLCTEPYTNTPPGSPCGCVWPMQIGL 314

Query: 139 RFSVPLYTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFD 198
           R SV LYTFFPLVSELAAEIAAGV+VK+SQVRVI ANAA QQPDKTV LIDLVPLGEKFD
Sbjct: 315 RLSVSLYTFFPLVSELAAEIAAGVFVKQSQVRVIGANAANQQPDKTVVLIDLVPLGEKFD 374

Query: 199 NTTAFLTYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNAR 258
           NTTAFLTYQRFWHKKVS+KA YFG YEVLYVRYPGLP+SPP G SG+DNEP+ +N+N+AR
Sbjct: 375 NTTAFLTYQRFWHKKVSVKASYFGKYEVLYVRYPGLPQSPPSGDSGIDNEPYYSNSNDAR 434

Query: 259 TVKPVGVDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDA----KPNPH 318
           TVKP+GVDV N Q KDRIS GMIAII LSTTVAV  C+ TAWIL+   ND     KP PH
Sbjct: 435 TVKPIGVDVQNIQHKDRISGGMIAIIALSTTVAVVLCVATAWILLLRRNDGVCQPKPTPH 494

Query: 319 VSLSALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRIL 378
             +S+LNKPSG GG VMA MPSSASLSFG SIA YSGSAK FSASDIER TNNFDPSRIL
Sbjct: 495 ALVSSLNKPSGAGGSVMAAMPSSASLSFGSSIAPYSGSAKTFSASDIERATNNFDPSRIL 554

Query: 379 GEGGFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEE 438
           GEGGFGRVY GTLEDG EVA+KVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEE
Sbjct: 555 GEGGFGRVYRGTLEDGTEVAVKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEE 614

Query: 439 HNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRD 498
            + SLVYEL+PNGSVESHLH +DKET  LDW ARMKIALGAARGLSYLHEDSSPRVIHRD
Sbjct: 615 GSRSLVYELIPNGSVESHLHDVDKETAPLDWDARMKIALGAARGLSYLHEDSSPRVIHRD 674

Query: 499 FKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDV 558
           FKSSNILLEHDFTPKVSDFGLARTA DEESRHI+T VMGTFGYVAPEYAMTGHLLVKSDV
Sbjct: 675 FKSSNILLEHDFTPKVSDFGLARTAMDEESRHISTRVMGTFGYVAPEYAMTGHLLVKSDV 734

Query: 559 YSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIA 618
           YSYGVVLLELLTGRKP+DMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDS+VPFENIA
Sbjct: 735 YSYGVVLLELLTGRKPVDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSSVPFENIA 794

Query: 619 KVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEAS 678
           KVAAIASMCVQPEVSHRPFM EVVQALKLVCNQC++ +E  SQ  SS  NS V+MNDE  
Sbjct: 795 KVAAIASMCVQPEVSHRPFMGEVVQALKLVCNQCNDAREAISQAGSSLDNSSVEMNDETL 854

Query: 679 SSKLMLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRI 734
           SS+ MLDSFHSEYLV  S+SGLD IDTRLS+S +LNTSI +GM ESRSL RC SS+PLR 
Sbjct: 855 SSRYMLDSFHSEYLVSGSMSGLDVIDTRLSMSGMLNTSIGIGMLESRSLERCLSSSPLRN 914

BLAST of Carg21960 vs. ExPASy Swiss-Prot
Match: Q8RWW0 (Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=3702 GN=ALE2 PE=1 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 3.4e-167
Identity = 358/715 (50.07%), Postives = 459/715 (64.20%), Query Frame = 0

Query: 29  PYISPAVALSPNNALPPLNPSPEEAPSPHNDMGHSHDAEQPILPPQEAHDVEQPNEDCSA 88
           PY++P+VA +P+   P  +P+P   P P    GH       +   + A  V   + DC  
Sbjct: 47  PYLNPSVAPTPS---PAFSPNPSRIP-PLRHKGHHRHRRWHL--RRNATAVSPSSHDCQQ 106

Query: 89  TLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPLYTFFPLVSELAAEIAAGVYVKRSQVRVI 148
           T C +P T+TP GSPCGCV+P++V+L  SV  ++ FP+ +EL  E+AAG Y+++SQV+++
Sbjct: 107 T-CVEPLTSTPFGSPCGCVFPMKVQLLLSVAPFSIFPVTNELEIEVAAGTYLEQSQVKIM 166

Query: 149 EANAAYQQPDKTVALIDLVPLGEKFDNTTAFLTYQRFWHKKVSIKAYYFGNYEVLYVRYP 208
            A+A  +   KTV  I+LVPLGEKFDNTTA L YQRF HKKV +    FG+YEV ++ YP
Sbjct: 167 GASADSENQGKTVVDINLVPLGEKFDNTTATLIYQRFRHKKVPLNETVFGDYEVTHISYP 226

Query: 209 GLPRSPPPGVSGLDNEPHLTNTNNARTVKPVGVDVHNRQPKDRISDGMIAIITLSTTVAV 268
           G+P S P G    D    L    NA T       +  R        G + I+ L   +++
Sbjct: 227 GIPSSSPNGDVTGDAPGGLPIPINATTFANKSQGIGFRTIAIIALSGFVLILVLVGAISI 286

Query: 269 GSCLVTAWILIFTHNDAKPNPHVSLSALNKPSGGGGLVMATMPSSASLSFGYSIASYSGS 328
               +  W  I   ++A   P ++ S +NK  G G +  ++  SS S S   S+A+ + S
Sbjct: 287 ----IVKWKKIGKSSNA-VGPALAPS-INKRPGAGSMFSSSARSSGSDSLMSSMATCALS 346

Query: 329 AKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAE 388
            K F+ S++E+ T+ F   R+LGEGGFGRVY G++EDG EVA+K+L RD+    REF+AE
Sbjct: 347 VKTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNRDREFIAE 406

Query: 389 VEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKIA 448
           VEMLSRLHHRNLVKLIGIC E     L+YELV NGSVESHLH      G LDW AR+KIA
Sbjct: 407 VEMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLH-----EGTLDWDARLKIA 466

Query: 449 LGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHVM 508
           LGAARGL+YLHEDS+PRVIHRDFK+SN+LLE DFTPKVSDFGLAR AT E S+HI+T VM
Sbjct: 467 LGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREAT-EGSQHISTRVM 526

Query: 509 GTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTS 568
           GTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGR+P+DMSQP GEENLV WARPLL +
Sbjct: 527 GTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLAN 586

Query: 569 KEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEV- 628
           +EGL+ ++D +L     F+++AKVAAIASMCV  EVSHRPFM EVVQALKL+ N  DE  
Sbjct: 587 REGLEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQALKLIYNDADETC 646

Query: 629 QETSSQTNSSSKNSFVDMNDEASSS--------KLMLDSFHSEYLVPNSISGLDGIDTRL 688
            +  SQ +SS  +S     D A S         +L      S   +  S   L+ ++ R 
Sbjct: 647 GDYCSQKDSSVPDSADFKGDLAPSDSSWWNLTPRLRYGQASSFITMDYSSGPLEDMENRP 706

Query: 689 SISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQRMRSSMSEHGMPRLS 735
             +  +     + +           S PLR  +S+RNF+ R+R SMSEHG P  S
Sbjct: 707 HSASSIPRVGGLILPN--------RSGPLRPMRSRRNFF-RLRGSMSEHGGPSSS 733

BLAST of Carg21960 vs. ExPASy Swiss-Prot
Match: Q9FE20 (Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana OX=3702 GN=PBS1 PE=1 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 1.1e-83
Identity = 180/404 (44.55%), Postives = 233/404 (57.67%), Query Frame = 0

Query: 289 PHVSLSALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSR 348
           P VS +    PSGG  L   T   S              +A  F+  ++   T NF P  
Sbjct: 31  PTVSNNISGLPSGGEKLSSKTNGGSKRELLLPRDGLGQIAAHTFAFRELAAATMNFHPDT 90

Query: 349 ILGEGGFGRVYSGTLED-GIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGIC 408
            LGEGGFGRVY G L+  G  VA+K L R+  QG REFL EV MLS LHH NLV LIG C
Sbjct: 91  FLGEGGFGRVYKGRLDSTGQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYC 150

Query: 409 TEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVI 468
            +     LVYE +P GS+E HLH +  +   LDW  RMKIA GAA+GL +LH+ ++P VI
Sbjct: 151 ADGDQRLLVYEFMPLGSLEDHLHDLPPDKEALDWNMRMKIAAGAAKGLEFLHDKANPPVI 210

Query: 469 HRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVK 528
           +RDFKSSNILL+  F PK+SDFGLA+     +  H++T VMGT+GY APEYAMTG L VK
Sbjct: 211 YRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTRVMGTYGYCAPEYAMTGQLTVK 270

Query: 529 SDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFE 588
           SDVYS+GVV LEL+TGRK ID   P GE+NLVAWARPL   +     + D  L    P  
Sbjct: 271 SDVYSFGVVFLELITGRKAIDSEMPHGEQNLVAWARPLFNDRRKFIKLADPRLKGRFPTR 330

Query: 589 NIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNS-FVDMN 648
            + +  A+ASMC+Q + + RP +++VV AL  + NQ  +  +  S+ N   + +  +  N
Sbjct: 331 ALYQALAVASMCIQEQAATRPLIADVVTALSYLANQAYDPSKDDSRRNRDERGARLITRN 390

Query: 649 DEASSSKLMLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSIN 691
           D+   S    D           + G +  D+    + +LN  IN
Sbjct: 391 DDGGGSGSKFD-----------LEGSEKEDSPRETARILNRDIN 423

BLAST of Carg21960 vs. ExPASy Swiss-Prot
Match: Q6I5Q6 (Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN=RLCK185 PE=1 SV=1)

HSP 1 Score: 310.1 bits (793), Expect = 6.9e-83
Identity = 162/323 (50.15%), Postives = 210/323 (65.02%), Query Frame = 0

Query: 328 SAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLA 387
           +A  F+  ++   T NF    +LGEGGFGRVY G LE+G  VA+K L R+  QG REFL 
Sbjct: 64  AAHTFTFRELAAATKNFRQDCLLGEGGFGRVYKGHLENGQAVAVKQLDRNGLQGNREFLV 123

Query: 388 EVEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKI 447
           EV MLS LHH NLV LIG C +     LVYE +P GS+E HLH I  +   LDW  RMKI
Sbjct: 124 EVLMLSLLHHDNLVNLIGYCADGDQRLLVYEFMPLGSLEDHLHDIPPDKEPLDWNTRMKI 183

Query: 448 ALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHV 507
           A GAA+GL +LH+ ++P VI+RDFKSSNILL   + PK+SDFGLA+     +  H++T V
Sbjct: 184 AAGAAKGLEFLHDKANPPVIYRDFKSSNILLGEGYHPKLSDFGLAKLGPVGDKTHVSTRV 243

Query: 508 MGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLT 567
           MGT+GY APEYAMTG L VKSDVYS+GVV LEL+TGRK ID ++P GE+NLVAWARPL  
Sbjct: 244 MGTYGYCAPEYAMTGQLTVKSDVYSFGVVFLELITGRKAIDNTKPLGEQNLVAWARPLFK 303

Query: 568 SKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDE- 627
            +     + D  L    P   + +  A+A+MC+Q + + RPF+ +VV AL  + +Q  + 
Sbjct: 304 DRRKFPKMADPLLAGRFPMRGLYQALAVAAMCLQEQAATRPFIGDVVTALSYLASQTYDP 363

Query: 628 ---VQETSSQTNSSSKNSFVDMN 647
              VQ + S  ++    + V  N
Sbjct: 364 NTPVQHSRSNASTPRARNRVGAN 386

BLAST of Carg21960 vs. ExPASy Swiss-Prot
Match: Q1PDV6 (Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 PE=1 SV=1)

HSP 1 Score: 305.4 bits (781), Expect = 1.7e-81
Identity = 162/326 (49.69%), Postives = 209/326 (64.11%), Query Frame = 0

Query: 328 SAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLE-DGIEVAIKVLKRDDHQGGREFL 387
           +A+ F+  ++   T NF P  +LGEGGFGRVY G LE  G  VA+K L R+  QG REFL
Sbjct: 67  AAQTFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFL 126

Query: 388 AEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMK 447
            EV MLS LHH NLV LIG C +     LVYE +P GS+E HLH +  +   LDW  RM 
Sbjct: 127 VEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMT 186

Query: 448 IALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTH 507
           IA GAA+GL YLH+ ++P VI+RD KSSNILL   + PK+SDFGLA+     +  H++T 
Sbjct: 187 IAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTR 246

Query: 508 VMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLL 567
           VMGT+GY APEYAMTG L +KSDVYS+GVV LEL+TGRK ID ++ PGE NLVAWARPL 
Sbjct: 247 VMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLF 306

Query: 568 TSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDE 627
             +     + D SL    P   + +  A+A+MC+Q + + RP + +VV AL  + +Q  +
Sbjct: 307 KDRRKFPKMADPSLQGRYPMRGLYQALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFD 366

Query: 628 VQETSSQ-TNSSSKNSFVDMNDEASS 652
               S Q + S S   F+   D+  S
Sbjct: 367 PNAPSGQNSRSGSGPPFIRTRDDRRS 392

BLAST of Carg21960 vs. ExPASy Swiss-Prot
Match: F4JEQ2 (Probable serine/threonine-protein kinase PBL23 OS=Arabidopsis thaliana OX=3702 GN=PBL23 PE=2 SV=1)

HSP 1 Score: 303.1 bits (775), Expect = 8.4e-81
Identity = 152/293 (51.88%), Postives = 201/293 (68.60%), Query Frame = 0

Query: 328 SAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGIE-VAIKVLKRDDHQGGREFL 387
           SA IF+  ++   T NF+P   LGEGGFGRVY G +E   + VA+K L R+ +QG REFL
Sbjct: 66  SAHIFTFRELCVATKNFNPDNQLGEGGFGRVYKGQIETPEQVVAVKQLDRNGYQGNREFL 125

Query: 388 AEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDK-ETGLLDWGARM 447
            EV MLS LHH+NLV L+G C +     LVYE + NGS+E HL  + + +   LDW  RM
Sbjct: 126 VEVMMLSLLHHQNLVNLVGYCADGDQRILVYEYMQNGSLEDHLLELARNKKKPLDWDTRM 185

Query: 448 KIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITT 507
           K+A GAARGL YLHE + P VI+RDFK+SNILL+ +F PK+SDFGLA+        H++T
Sbjct: 186 KVAAGAARGLEYLHETADPPVIYRDFKASNILLDEEFNPKLSDFGLAKVGPTGGETHVST 245

Query: 508 HVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPL 567
            VMGT+GY APEYA+TG L VKSDVYS+GVV LE++TGR+ ID ++P  E+NLV WA PL
Sbjct: 246 RVMGTYGYCAPEYALTGQLTVKSDVYSFGVVFLEMITGRRVIDTTKPTEEQNLVTWASPL 305

Query: 568 LTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALK 619
              +    ++ D  L+   P + + +  A+A+MC+Q E + RP MS+VV AL+
Sbjct: 306 FKDRRKFTLMADPLLEGKYPIKGLYQALAVAAMCLQEEAATRPMMSDVVTALE 358

BLAST of Carg21960 vs. ExPASy TrEMBL
Match: A0A6J1EZE7 (receptor-like serine/threonine-protein kinase ALE2 OS=Cucurbita moschata OX=3662 GN=LOC111440514 PE=4 SV=1)

HSP 1 Score: 1432.9 bits (3708), Expect = 0.0e+00
Identity = 726/737 (98.51%), Postives = 732/737 (99.32%), Query Frame = 0

Query: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60
           MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLN SPEEAPSPHNDM
Sbjct: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNHSPEEAPSPHNDM 60

Query: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120
           GHSHDA+QPILP QEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL
Sbjct: 61  GHSHDADQPILPSQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120

Query: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180
           YTFFPLVSELAAEIA+GVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL
Sbjct: 121 YTFFPLVSELAAEIASGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180

Query: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240
           TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG
Sbjct: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240

Query: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300
           VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS
Sbjct: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300

Query: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360
           GGGG VMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS
Sbjct: 301 GGGGSVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360

Query: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420
           GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV
Sbjct: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420

Query: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480
           PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH
Sbjct: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480

Query: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540
           DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL
Sbjct: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540

Query: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600
           LTGRKP+DMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV
Sbjct: 541 LTGRKPVDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600

Query: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660
           QPEVSHRPFMSEVVQALKLVCNQCDEV+ETSSQTNSSSKNSFV+MNDEASSSKL+LDSFH
Sbjct: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVRETSSQTNSSSKNSFVNMNDEASSSKLVLDSFH 660

Query: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720
           SEYLVPNSISGLDGIDTRLSISDLLNTSI MGMQESRSLRRCFSSAPLRIEKSKRNFWQR
Sbjct: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSIYMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720

Query: 721 MRSSMSEHGMPRLSHTP 738
           MRSSM EHGMPRLSHTP
Sbjct: 721 MRSSMREHGMPRLSHTP 737

BLAST of Carg21960 vs. ExPASy TrEMBL
Match: A0A6J1HSS7 (receptor-like serine/threonine-protein kinase ALE2 OS=Cucurbita maxima OX=3661 GN=LOC111466443 PE=4 SV=1)

HSP 1 Score: 1398.6 bits (3619), Expect = 0.0e+00
Identity = 709/737 (96.20%), Postives = 722/737 (97.96%), Query Frame = 0

Query: 1   MEVLVPTILHLLILLVTVHASELRDIAEPYISPAVALSPNNALPPLNPSPEEAPSPHNDM 60
           MEVLVPT+LHLLILLVTVHASELR+IAEPYISPAVALSPNNALPPLN SPEEAPS HNDM
Sbjct: 1   MEVLVPTMLHLLILLVTVHASELREIAEPYISPAVALSPNNALPPLNHSPEEAPSTHNDM 60

Query: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120
           GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL
Sbjct: 61  GHSHDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPL 120

Query: 121 YTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180
           YTFFPLVSELAAEIA+GVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL
Sbjct: 121 YTFFPLVSELAAEIASGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFL 180

Query: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKPVG 240
           TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNN RTVKPVG
Sbjct: 181 TYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNVRTVKPVG 240

Query: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDAKPNPHVSLSALNKPS 300
           VDVHNRQPKDRISDGMIAIITLSTTVAVG CLVTAWIL+FTHNDAKPNPHVSLSALNKPS
Sbjct: 241 VDVHNRQPKDRISDGMIAIITLSTTVAVGLCLVTAWILLFTHNDAKPNPHVSLSALNKPS 300

Query: 301 GGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360
           G GG VMAT+PSSASL FGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS
Sbjct: 301 GAGGSVMATIPSSASLFFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYS 360

Query: 361 GTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420
           GTLEDG EVAIKVLKRDDHQG REFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV
Sbjct: 361 GTLEDGTEVAIKVLKRDDHQGSREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELV 420

Query: 421 PNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEH 480
           PNGSVESHLHG+DKETGLLDWGARMKIALGAARGLSYLHEDS PRVIHRDFKSSNILLEH
Sbjct: 421 PNGSVESHLHGMDKETGLLDWGARMKIALGAARGLSYLHEDSIPRVIHRDFKSSNILLEH 480

Query: 481 DFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540
           +FTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL
Sbjct: 481 NFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLEL 540

Query: 541 LTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600
           LTGRKPIDMSQPPGEE+LVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV
Sbjct: 541 LTGRKPIDMSQPPGEESLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCV 600

Query: 601 QPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFH 660
           QPEVSHRPFM EVVQALKLVCNQCD VQETSSQTNSSSKNS VDMNDEASSSKL+LDSFH
Sbjct: 601 QPEVSHRPFMGEVVQALKLVCNQCDGVQETSSQTNSSSKNSSVDMNDEASSSKLVLDSFH 660

Query: 661 SEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQR 720
           S+YLVPNSISGLDGIDTRLSISDLLNTSI+MGMQESRSL+RCFSSA LRIEKSKRN+WQR
Sbjct: 661 SKYLVPNSISGLDGIDTRLSISDLLNTSIDMGMQESRSLKRCFSSATLRIEKSKRNYWQR 720

Query: 721 MRSSMSEHGMPRLSHTP 738
           M SSMSEHGMPRLSHTP
Sbjct: 721 MISSMSEHGMPRLSHTP 737

BLAST of Carg21960 vs. ExPASy TrEMBL
Match: A0A5D3CEX4 (Receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G002250 PE=4 SV=1)

HSP 1 Score: 1085.5 bits (2806), Expect = 0.0e+00
Identity = 572/743 (76.99%), Postives = 618/743 (83.18%), Query Frame = 0

Query: 13   ILLVTVHASELRDIAEPYISPAVALSPNN-ALPPLNPSPEEAPSPH-NDMGHS------- 72
            ++    H+   R  + P  S    +SP++ A PPL+     +PSPH    GH+       
Sbjct: 301  VISTAQHSPGRRHHSPPPKSEPSPISPSHYAPPPLSTHVSPSPSPHQKTSGHTRRHFYPP 360

Query: 73   ------HDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFS 132
                  H A+ P + P        PNEDC  T C +PYTNTPPGSPCGCVWP+Q+ LR S
Sbjct: 361  KASPSKHSAKSPKMSPIHTLPPPPPNEDCFTTQCIEPYTNTPPGSPCGCVWPMQIGLRLS 420

Query: 133  VPLYTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTT 192
            V LYTFFPLVSELAAEIAAGV+VK+SQVR+I ANAA QQPDKTV LIDLVPLGEKFDNTT
Sbjct: 421  VSLYTFFPLVSELAAEIAAGVFVKQSQVRIIGANAANQQPDKTVILIDLVPLGEKFDNTT 480

Query: 193  AFLTYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVK 252
            AFLTYQRFWHKKVSIKA YFGNYEVLYVRYPGLP+SPP G SGLDNEP+  N+N+AR VK
Sbjct: 481  AFLTYQRFWHKKVSIKASYFGNYEVLYVRYPGLPQSPPSGDSGLDNEPYFGNSNDARAVK 540

Query: 253  PVGVDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDA----KPNPHVSL 312
            P+GVDV NRQ KDR+S GMIAI+ LSTTVAV  C+ TAWIL+F  ND     KP PHVSL
Sbjct: 541  PIGVDVQNRQHKDRLSGGMIAIVALSTTVAVVLCVATAWILLFKRNDGLCQPKPTPHVSL 600

Query: 313  SALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEG 372
            S+LNKPSG GG VMATMPSSASLS G SIA YSGSAK FSASDIER TNNFDPSRILGEG
Sbjct: 601  SSLNKPSGAGGSVMATMPSSASLSIGSSIAPYSGSAKTFSASDIERATNNFDPSRILGEG 660

Query: 373  GFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNC 432
            GFGRVY G LEDG EVA+KVLKRDD QGGREFLAEVEMLSRLHHRNLVKLIGICTEE + 
Sbjct: 661  GFGRVYRGILEDGTEVAVKVLKRDDLQGGREFLAEVEMLSRLHHRNLVKLIGICTEERSR 720

Query: 433  SLVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKS 492
            SLVYEL+PNGSVESHLHG+DKET  LDW AR+KIALGAARGLSYLHEDSSPRVIHRDFKS
Sbjct: 721  SLVYELIPNGSVESHLHGVDKETAPLDWEARIKIALGAARGLSYLHEDSSPRVIHRDFKS 780

Query: 493  SNILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSY 552
            SNILLEHDFTPKVSDFGLARTA DEESRHI+T VMGTFGYVAPEYAMTGHLLVKSDVYSY
Sbjct: 781  SNILLEHDFTPKVSDFGLARTAMDEESRHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSY 840

Query: 553  GVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVA 612
            GVVLLELLTGRKP+DMSQPPGEENLV+WARPLLTSKEGLDVIIDKSLDSNVPFENIAKVA
Sbjct: 841  GVVLLELLTGRKPVDMSQPPGEENLVSWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVA 900

Query: 613  AIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMN-DEASSS 672
            AIASMCVQPEVSHRPFM EVVQALKLVCNQC+  QETSS   SS ++S +D N DEASSS
Sbjct: 901  AIASMCVQPEVSHRPFMGEVVQALKLVCNQCNTTQETSSHAGSSPESSTIDTNDDEASSS 960

Query: 673  KLMLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEK 732
            + MLDSFHSEYLVPNSI GLD IDT LSISDLLNTSI+ G QESRSL RCFSS+P RI K
Sbjct: 961  RYMLDSFHSEYLVPNSIPGLDSIDTHLSISDLLNTSIDNGKQESRSLERCFSSSPHRIRK 1020

BLAST of Carg21960 vs. ExPASy TrEMBL
Match: A0A1S4DYF0 (receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo OX=3656 GN=LOC103492328 PE=4 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 571/743 (76.85%), Postives = 617/743 (83.04%), Query Frame = 0

Query: 13   ILLVTVHASELRDIAEPYISPAVALSPNN-ALPPLNPSPEEAPSPH-NDMGHS------- 72
            ++    H+   R  + P  S    +SP++ A PPL+     +PSPH    GH+       
Sbjct: 301  VISTAQHSPGRRHHSPPPKSEPSPISPSHYAPPPLSTHVSPSPSPHQKTSGHTRRHFYPP 360

Query: 73   ------HDAEQPILPPQEAHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFS 132
                  H A+ P + P        PNEDC  T C +PYTNTPPGSPCGCVWP+Q+ LR S
Sbjct: 361  KASPSKHSAKSPKMSPIHTLPPPPPNEDCFTTQCIEPYTNTPPGSPCGCVWPMQIGLRLS 420

Query: 133  VPLYTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTT 192
            V LYTFFPLVSELAAEIAAGV+VK+SQVR+I ANAA QQPDKTV LIDLVPLGEKFDNTT
Sbjct: 421  VSLYTFFPLVSELAAEIAAGVFVKQSQVRIIGANAANQQPDKTVILIDLVPLGEKFDNTT 480

Query: 193  AFLTYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVK 252
            AFLTYQRFWHKKVSIKA YFGNYEVLYVRYPGLP+SPP G SGLDNEP+  N+N+AR VK
Sbjct: 481  AFLTYQRFWHKKVSIKASYFGNYEVLYVRYPGLPQSPPSGDSGLDNEPYFGNSNDARAVK 540

Query: 253  PVGVDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDA----KPNPHVSL 312
            P+GVDV NRQ KDR+S GMIAI+ LSTTVAV  C+ TAWIL+F  ND     KP PHVSL
Sbjct: 541  PIGVDVQNRQHKDRLSGGMIAIVALSTTVAVVLCVATAWILLFKRNDGLCQPKPTPHVSL 600

Query: 313  SALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEG 372
            S+LNKPSG GG VMATMPSSASLS G SIA YSGSAK FSASDIER TNNFDPSRILGEG
Sbjct: 601  SSLNKPSGAGGSVMATMPSSASLSIGSSIAPYSGSAKTFSASDIERATNNFDPSRILGEG 660

Query: 373  GFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNC 432
            GFGRVY G LEDG EVA+KVLKRDD QGGREFLAEVEMLSRLHHRNLVKLIGICTEE + 
Sbjct: 661  GFGRVYRGILEDGTEVAVKVLKRDDLQGGREFLAEVEMLSRLHHRNLVKLIGICTEERSR 720

Query: 433  SLVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKS 492
            SLVYEL+PNGSVESHLHG+DKET  LDW AR+KIALGAARGLSYLHEDSSPRVIHRDFKS
Sbjct: 721  SLVYELIPNGSVESHLHGVDKETAPLDWEARIKIALGAARGLSYLHEDSSPRVIHRDFKS 780

Query: 493  SNILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSY 552
            SNILLEHDFTPKVSDFGLARTA DEESRHI+T VMGTFGYVAPEYAMTGHLLVKSDVYSY
Sbjct: 781  SNILLEHDFTPKVSDFGLARTAMDEESRHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSY 840

Query: 553  GVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVA 612
            GVVLLELLTGRKP+DMSQPPGEENLV+WARPLLTSKEGLDVIIDKSLDSNVPFENIAKVA
Sbjct: 841  GVVLLELLTGRKPVDMSQPPGEENLVSWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVA 900

Query: 613  AIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMN-DEASSS 672
            AIASMCVQPEVSHRPFM EVVQALKLVCNQC+  QETSS   SS ++S +D N DEASSS
Sbjct: 901  AIASMCVQPEVSHRPFMGEVVQALKLVCNQCNTTQETSSHPGSSPESSTIDTNDDEASSS 960

Query: 673  KLMLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEK 732
            + MLD FHSEYLVPNSI GLD IDT LSISDLLNTSI+ G QESRSL RCFSS+P RI K
Sbjct: 961  RYMLDFFHSEYLVPNSIPGLDSIDTHLSISDLLNTSIDNGKQESRSLERCFSSSPHRIRK 1020

BLAST of Carg21960 vs. ExPASy TrEMBL
Match: A0A6J1JEQ4 (receptor-like serine/threonine-protein kinase ALE2 OS=Cucurbita maxima OX=3661 GN=LOC111485108 PE=4 SV=1)

HSP 1 Score: 1074.7 bits (2778), Expect = 2.7e-310
Identity = 571/743 (76.85%), Postives = 618/743 (83.18%), Query Frame = 0

Query: 19  HASELRDIAEPYISPAVALSPNN-ALPPLNPSPEEAPSPHNDM-GHSHD----------- 78
           H+ E  D   P  S   ++SP++   PPL      +PSP+  M GH+             
Sbjct: 195 HSHERGDYTPPMESDPSSISPSHYDHPPLVTHVSPSPSPYQKMSGHTERHFYPPKASPSP 254

Query: 79  ----AEQPILPP-QEAHDV--EQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSV 138
               A+ P +PP +  H +    PNEDCSATLCT+PYTNTPPGSPCGCVWP+Q+ LR SV
Sbjct: 255 LKPWAKSPKMPPLRSIHTLPPPPPNEDCSATLCTEPYTNTPPGSPCGCVWPMQIGLRLSV 314

Query: 139 PLYTFFPLVSELAAEIAAGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTA 198
            LYTFFPLVSELAAEIAAGV+VK+SQVRVI ANAA QQPDKTV LIDLVPLGEKFDNTTA
Sbjct: 315 SLYTFFPLVSELAAEIAAGVFVKQSQVRVIGANAANQQPDKTVVLIDLVPLGEKFDNTTA 374

Query: 199 FLTYQRFWHKKVSIKAYYFGNYEVLYVRYPGLPRSPPPGVSGLDNEPHLTNTNNARTVKP 258
           FLTYQRFWHKKVS+KA YFG YEVLYVRYPGLP+SPP G SG+DNEP+ +N+N+ARTVKP
Sbjct: 375 FLTYQRFWHKKVSVKASYFGKYEVLYVRYPGLPQSPPSGDSGIDNEPYYSNSNDARTVKP 434

Query: 259 VGVDVHNRQPKDRISDGMIAIITLSTTVAVGSCLVTAWILIFTHNDA----KPNPHVSLS 318
           +GVDV N Q KDRIS GMIAII LSTTVAV  C+ TAWIL+   ND     KP PH  +S
Sbjct: 435 IGVDVQNIQHKDRISGGMIAIIALSTTVAVVLCVATAWILLLRCNDGVCQPKPTPHALVS 494

Query: 319 ALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGG 378
           +LNKPSG GG VMA MPSSASLSFG SIA YSGSAK FSASDIER TNNFDPSRILGEGG
Sbjct: 495 SLNKPSGAGGSVMAAMPSSASLSFGSSIAPYSGSAKTFSASDIERATNNFDPSRILGEGG 554

Query: 379 FGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCS 438
           FGRVY GTLEDG EVA+KVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEE + S
Sbjct: 555 FGRVYRGTLEDGPEVAVKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEGSRS 614

Query: 439 LVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSS 498
           LVYEL+PNGSVESHLH +DKET  LDW ARMKIALGAARGLSYLHEDSSPRVIHRDFKSS
Sbjct: 615 LVYELIPNGSVESHLHDVDKETAPLDWDARMKIALGAARGLSYLHEDSSPRVIHRDFKSS 674

Query: 499 NILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYG 558
           NILLEHDFTPKVSDFGLARTA DEESRHI+T VMGTFGYVAPEYAMTGHLLVKSDVYSYG
Sbjct: 675 NILLEHDFTPKVSDFGLARTAMDEESRHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSYG 734

Query: 559 VVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAA 618
           VVLLELLTGRKP+DMSQP GEENLVAWARPLLTSKEGLDVIIDKSLDS+VPFENIAKVAA
Sbjct: 735 VVLLELLTGRKPVDMSQPSGEENLVAWARPLLTSKEGLDVIIDKSLDSSVPFENIAKVAA 794

Query: 619 IASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKL 678
           IASMCVQPEVSHRPFM EVVQALKLVCNQC++ +E  SQ  SS  NS V+MNDE  SS+ 
Sbjct: 795 IASMCVQPEVSHRPFMGEVVQALKLVCNQCNDAREAISQAGSSLDNSSVEMNDETLSSRY 854

Query: 679 MLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSK 734
           MLDSF+SEYLV  SISGLD IDTRLSIS +LNTSI +GM ESRSL RC SS+PLR  K K
Sbjct: 855 MLDSFNSEYLVSGSISGLDVIDTRLSISGMLNTSIGIGMLESRSLERCLSSSPLRNGKGK 914

BLAST of Carg21960 vs. TAIR 10
Match: AT5G56890.1 (Protein kinase superfamily protein )

HSP 1 Score: 783.1 bits (2021), Expect = 2.0e-226
Identity = 437/729 (59.95%), Postives = 518/729 (71.06%), Query Frame = 0

Query: 19   HASELRDIAEPYISPA---VALSPNNALPPLNPSPEEAPSPHNDMGHSHDAEQPILPPQE 78
            H +    I +  ISPA   V+ S     P L PSP +     +    S   + P+ PP +
Sbjct: 388  HHAPSPPIPDSLISPAHAPVSFSMKRISPALAPSPTQVFPLRSSSRPSKSRKFPLGPPLQ 447

Query: 79   AHDVEQPNEDCSATLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPLYTFFPLVSELAAEIA 138
            A     PN DCS+T+C +PYTNTPPGSPCGCVWPIQV LR S+ LY FFP+VSE A EI+
Sbjct: 448  AFPPPPPNSDCSSTICLEPYTNTPPGSPCGCVWPIQVELRLSMALYDFFPMVSEFAREIS 507

Query: 139  AGVYVKRSQVRVIEANAAYQQPDKTVALIDLVPLGEKFDNTTAFLTYQRFWHKKVSIKAY 198
            AGV++K+SQVR++ ANAA +QP+K++ LIDLVPLG+KFDN TA LTYQRFW KKV I   
Sbjct: 508  AGVFMKQSQVRIMGANAASEQPEKSIVLIDLVPLGDKFDNMTAMLTYQRFWSKKVYIDEP 567

Query: 199  YFGNYEVLYVRYPGLPRSPP-PGVSGLDNEPHLTNTNNARTVKPVGVDVHNRQPKDRISD 258
             FG Y+V+YVRYPGLP SPP  G++ +D  P+  N NN R VKP+GVDV  +  K  ++ 
Sbjct: 568  IFGGYDVIYVRYPGLPASPPTSGMTIIDQGPYSGN-NNGRAVKPLGVDVPRKPRKKELNG 627

Query: 259  GMIAIITLSTTVAVGSCLVTAWILIFTH-------NDAKPNPHVSLSALNKPSGGGGLVM 318
            G IA+I LS    +G C V  W L+F         +   P    SL +L+KPSG    + 
Sbjct: 628  GSIAVIVLSAAAFIGLCFVIVWFLVFRRQRDRRRLSKRTPLARPSLPSLSKPSGSARSLT 687

Query: 319  ATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGI 378
             +  SS SLSF  SIA ++ SAK F+AS+I + TNNFD SR+LGEGGFGRVY G  +DG 
Sbjct: 688  GSRFSSTSLSFESSIAPFTLSAKTFTASEIMKATNNFDESRVLGEGGFGRVYEGVFDDGT 747

Query: 379  EVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVES 438
            +VA+KVLKRDD QG REFLAEVEMLSRLHHRNLV LIGIC E+ N SLVYEL+PNGSVES
Sbjct: 748  KVAVKVLKRDDQQGSREFLAEVEMLSRLHHRNLVNLIGICIEDRNRSLVYELIPNGSVES 807

Query: 439  HLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVS 498
            HLHGIDK +  LDW AR+KIALGAARGL+YLHEDSSPRVIHRDFKSSNILLE+DFTPKVS
Sbjct: 808  HLHGIDKASSPLDWDARLKIALGAARGLAYLHEDSSPRVIHRDFKSSNILLENDFTPKVS 867

Query: 499  DFGLARTA-TDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKP 558
            DFGLAR A  DE++RHI+T VMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKP
Sbjct: 868  DFGLARNALDDEDNRHISTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKP 927

Query: 559  IDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSH 618
            +DMSQPPG+ENLV+W RP LTS EGL  IID+SL   + F++IAKVAAIASMCVQPEVSH
Sbjct: 928  VDMSQPPGQENLVSWTRPFLTSAEGLAAIIDQSLGPEISFDSIAKVAAIASMCVQPEVSH 987

Query: 619  RPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNSFVDMNDEASSSKLMLDSFHSEYLVP 678
            RPFM EVVQALKLV N+CDE +E +S T S SK+ F D     SS            L+P
Sbjct: 988  RPFMGEVVQALKLVSNECDEAKELNSLT-SISKDDFRDDTQAESSCGDSSARMARYPLLP 1047

Query: 679  NSISGLDGIDTRLSISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQRMR---- 732
            N  S  D  +  LS S++   S   G  E +S     +S PL   + K +FWQ+MR    
Sbjct: 1048 NYDSEPD-TERGLSTSEMYTGS---GRFERQS-----NSGPLTSGRGK-SFWQKMRRLST 1104

BLAST of Carg21960 vs. TAIR 10
Match: AT2G20300.1 (Protein kinase superfamily protein )

HSP 1 Score: 590.1 bits (1520), Expect = 2.4e-168
Identity = 358/715 (50.07%), Postives = 459/715 (64.20%), Query Frame = 0

Query: 29  PYISPAVALSPNNALPPLNPSPEEAPSPHNDMGHSHDAEQPILPPQEAHDVEQPNEDCSA 88
           PY++P+VA +P+   P  +P+P   P P    GH       +   + A  V   + DC  
Sbjct: 47  PYLNPSVAPTPS---PAFSPNPSRIP-PLRHKGHHRHRRWHL--RRNATAVSPSSHDCQQ 106

Query: 89  TLCTDPYTNTPPGSPCGCVWPIQVRLRFSVPLYTFFPLVSELAAEIAAGVYVKRSQVRVI 148
           T C +P T+TP GSPCGCV+P++V+L  SV  ++ FP+ +EL  E+AAG Y+++SQV+++
Sbjct: 107 T-CVEPLTSTPFGSPCGCVFPMKVQLLLSVAPFSIFPVTNELEIEVAAGTYLEQSQVKIM 166

Query: 149 EANAAYQQPDKTVALIDLVPLGEKFDNTTAFLTYQRFWHKKVSIKAYYFGNYEVLYVRYP 208
            A+A  +   KTV  I+LVPLGEKFDNTTA L YQRF HKKV +    FG+YEV ++ YP
Sbjct: 167 GASADSENQGKTVVDINLVPLGEKFDNTTATLIYQRFRHKKVPLNETVFGDYEVTHISYP 226

Query: 209 GLPRSPPPGVSGLDNEPHLTNTNNARTVKPVGVDVHNRQPKDRISDGMIAIITLSTTVAV 268
           G+P S P G    D    L    NA T       +  R        G + I+ L   +++
Sbjct: 227 GIPSSSPNGDVTGDAPGGLPIPINATTFANKSQGIGFRTIAIIALSGFVLILVLVGAISI 286

Query: 269 GSCLVTAWILIFTHNDAKPNPHVSLSALNKPSGGGGLVMATMPSSASLSFGYSIASYSGS 328
               +  W  I   ++A   P ++ S +NK  G G +  ++  SS S S   S+A+ + S
Sbjct: 287 ----IVKWKKIGKSSNA-VGPALAPS-INKRPGAGSMFSSSARSSGSDSLMSSMATCALS 346

Query: 329 AKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGIEVAIKVLKRDDHQGGREFLAE 388
            K F+ S++E+ T+ F   R+LGEGGFGRVY G++EDG EVA+K+L RD+    REF+AE
Sbjct: 347 VKTFTLSELEKATDRFSAKRVLGEGGFGRVYQGSMEDGTEVAVKLLTRDNQNRDREFIAE 406

Query: 389 VEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKIA 448
           VEMLSRLHHRNLVKLIGIC E     L+YELV NGSVESHLH      G LDW AR+KIA
Sbjct: 407 VEMLSRLHHRNLVKLIGICIEGRTRCLIYELVHNGSVESHLH-----EGTLDWDARLKIA 466

Query: 449 LGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHVM 508
           LGAARGL+YLHEDS+PRVIHRDFK+SN+LLE DFTPKVSDFGLAR AT E S+HI+T VM
Sbjct: 467 LGAARGLAYLHEDSNPRVIHRDFKASNVLLEDDFTPKVSDFGLAREAT-EGSQHISTRVM 526

Query: 509 GTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTS 568
           GTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGR+P+DMSQP GEENLV WARPLL +
Sbjct: 527 GTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRRPVDMSQPSGEENLVTWARPLLAN 586

Query: 569 KEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEV- 628
           +EGL+ ++D +L     F+++AKVAAIASMCV  EVSHRPFM EVVQALKL+ N  DE  
Sbjct: 587 REGLEQLVDPALAGTYNFDDMAKVAAIASMCVHQEVSHRPFMGEVVQALKLIYNDADETC 646

Query: 629 QETSSQTNSSSKNSFVDMNDEASSS--------KLMLDSFHSEYLVPNSISGLDGIDTRL 688
            +  SQ +SS  +S     D A S         +L      S   +  S   L+ ++ R 
Sbjct: 647 GDYCSQKDSSVPDSADFKGDLAPSDSSWWNLTPRLRYGQASSFITMDYSSGPLEDMENRP 706

Query: 689 SISDLLNTSINMGMQESRSLRRCFSSAPLRIEKSKRNFWQRMRSSMSEHGMPRLS 735
             +  +     + +           S PLR  +S+RNF+ R+R SMSEHG P  S
Sbjct: 707 HSASSIPRVGGLILPN--------RSGPLRPMRSRRNFF-RLRGSMSEHGGPSSS 733

BLAST of Carg21960 vs. TAIR 10
Match: AT4G02010.1 (Protein kinase superfamily protein )

HSP 1 Score: 360.1 bits (923), Expect = 4.1e-99
Identity = 191/341 (56.01%), Postives = 236/341 (69.21%), Query Frame = 0

Query: 317 SFGYSIASYSGSAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLEDGIEVAIKVLKR 376
           SFG S+  +  S +  S  +++  T+NF+ + ILGEGGFG+VY G L DG  VAIK L  
Sbjct: 354 SFGGSL-PHPASTRFLSYEELKEATSNFESASILGEGGFGKVYRGILADGTAVAIKKLTS 413

Query: 377 DDHQGGREFLAEVEMLSRLHHRNLVKLIGICTEEHNCS--LVYELVPNGSVESHLHGIDK 436
              QG +EF  E++MLSRLHHRNLVKL+G  +   +    L YELVPNGS+E+ LHG   
Sbjct: 414 GGPQGDKEFQVEIDMLSRLHHRNLVKLVGYYSSRDSSQHLLCYELVPNGSLEAWLHGPLG 473

Query: 437 ETGLLDWGARMKIALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLART 496
               LDW  RMKIAL AARGL+YLHEDS P VIHRDFK+SNILLE++F  KV+DFGLA+ 
Sbjct: 474 LNCPLDWDTRMKIALDAARGLAYLHEDSQPSVIHRDFKASNILLENNFNAKVADFGLAKQ 533

Query: 497 ATDEESRHITTHVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPG 556
           A +    H++T VMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKP+DMSQP G
Sbjct: 534 APEGRGNHLSTRVMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPVDMSQPSG 593

Query: 557 EENLVAWARPLLTSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVV 616
           +ENLV W RP+L  K+ L+ ++D  L+   P E+  +V  IA+ CV PE S RP M EVV
Sbjct: 594 QENLVTWTRPVLRDKDRLEELVDSRLEGKYPKEDFIRVCTIAAACVAPEASQRPTMGEVV 653

Query: 617 QALKL---VCNQCDEVQETSSQTNSSSKNSFVDMNDEASSS 653
           Q+LK+   V    D V  TS++   + + S      E +SS
Sbjct: 654 QSLKMVQRVVEYQDPVLNTSNKARPNRRQSSATFESEVTSS 693

BLAST of Carg21960 vs. TAIR 10
Match: AT5G13160.1 (Protein kinase superfamily protein )

HSP 1 Score: 312.8 bits (800), Expect = 7.5e-85
Identity = 180/404 (44.55%), Postives = 233/404 (57.67%), Query Frame = 0

Query: 289 PHVSLSALNKPSGGGGLVMATMPSSASLSFGYSIASYSGSAKIFSASDIERTTNNFDPSR 348
           P VS +    PSGG  L   T   S              +A  F+  ++   T NF P  
Sbjct: 31  PTVSNNISGLPSGGEKLSSKTNGGSKRELLLPRDGLGQIAAHTFAFRELAAATMNFHPDT 90

Query: 349 ILGEGGFGRVYSGTLED-GIEVAIKVLKRDDHQGGREFLAEVEMLSRLHHRNLVKLIGIC 408
            LGEGGFGRVY G L+  G  VA+K L R+  QG REFL EV MLS LHH NLV LIG C
Sbjct: 91  FLGEGGFGRVYKGRLDSTGQVVAVKQLDRNGLQGNREFLVEVLMLSLLHHPNLVNLIGYC 150

Query: 409 TEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMKIALGAARGLSYLHEDSSPRVI 468
            +     LVYE +P GS+E HLH +  +   LDW  RMKIA GAA+GL +LH+ ++P VI
Sbjct: 151 ADGDQRLLVYEFMPLGSLEDHLHDLPPDKEALDWNMRMKIAAGAAKGLEFLHDKANPPVI 210

Query: 469 HRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTHVMGTFGYVAPEYAMTGHLLVK 528
           +RDFKSSNILL+  F PK+SDFGLA+     +  H++T VMGT+GY APEYAMTG L VK
Sbjct: 211 YRDFKSSNILLDEGFHPKLSDFGLAKLGPTGDKSHVSTRVMGTYGYCAPEYAMTGQLTVK 270

Query: 529 SDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLLTSKEGLDVIIDKSLDSNVPFE 588
           SDVYS+GVV LEL+TGRK ID   P GE+NLVAWARPL   +     + D  L    P  
Sbjct: 271 SDVYSFGVVFLELITGRKAIDSEMPHGEQNLVAWARPLFNDRRKFIKLADPRLKGRFPTR 330

Query: 589 NIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDEVQETSSQTNSSSKNS-FVDMN 648
            + +  A+ASMC+Q + + RP +++VV AL  + NQ  +  +  S+ N   + +  +  N
Sbjct: 331 ALYQALAVASMCIQEQAATRPLIADVVTALSYLANQAYDPSKDDSRRNRDERGARLITRN 390

Query: 649 DEASSSKLMLDSFHSEYLVPNSISGLDGIDTRLSISDLLNTSIN 691
           D+   S    D           + G +  D+    + +LN  IN
Sbjct: 391 DDGGGSGSKFD-----------LEGSEKEDSPRETARILNRDIN 423

BLAST of Carg21960 vs. TAIR 10
Match: AT5G18610.1 (Protein kinase superfamily protein )

HSP 1 Score: 305.4 bits (781), Expect = 1.2e-82
Identity = 162/326 (49.69%), Postives = 209/326 (64.11%), Query Frame = 0

Query: 328 SAKIFSASDIERTTNNFDPSRILGEGGFGRVYSGTLE-DGIEVAIKVLKRDDHQGGREFL 387
           +A+ F+  ++   T NF P  +LGEGGFGRVY G LE  G  VA+K L R+  QG REFL
Sbjct: 67  AAQTFTFRELAAATKNFRPECLLGEGGFGRVYKGRLETTGQIVAVKQLDRNGLQGNREFL 126

Query: 388 AEVEMLSRLHHRNLVKLIGICTEEHNCSLVYELVPNGSVESHLHGIDKETGLLDWGARMK 447
            EV MLS LHH NLV LIG C +     LVYE +P GS+E HLH +  +   LDW  RM 
Sbjct: 127 VEVLMLSLLHHPNLVNLIGYCADGDQRLLVYEYMPLGSLEDHLHDLPPDKEPLDWSTRMT 186

Query: 448 IALGAARGLSYLHEDSSPRVIHRDFKSSNILLEHDFTPKVSDFGLARTATDEESRHITTH 507
           IA GAA+GL YLH+ ++P VI+RD KSSNILL   + PK+SDFGLA+     +  H++T 
Sbjct: 187 IAAGAAKGLEYLHDKANPPVIYRDLKSSNILLGDGYHPKLSDFGLAKLGPVGDKTHVSTR 246

Query: 508 VMGTFGYVAPEYAMTGHLLVKSDVYSYGVVLLELLTGRKPIDMSQPPGEENLVAWARPLL 567
           VMGT+GY APEYAMTG L +KSDVYS+GVV LEL+TGRK ID ++ PGE NLVAWARPL 
Sbjct: 247 VMGTYGYCAPEYAMTGQLTLKSDVYSFGVVFLELITGRKAIDNARAPGEHNLVAWARPLF 306

Query: 568 TSKEGLDVIIDKSLDSNVPFENIAKVAAIASMCVQPEVSHRPFMSEVVQALKLVCNQCDE 627
             +     + D SL    P   + +  A+A+MC+Q + + RP + +VV AL  + +Q  +
Sbjct: 307 KDRRKFPKMADPSLQGRYPMRGLYQALAVAAMCLQEQAATRPLIGDVVTALTYLASQTFD 366

Query: 628 VQETSSQ-TNSSSKNSFVDMNDEASS 652
               S Q + S S   F+   D+  S
Sbjct: 367 PNAPSGQNSRSGSGPPFIRTRDDRRS 392

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6588041.10.0e+00100.00Receptor-like serine/threonine-protein kinase ALE2, partial [Cucurbita argyrospe... [more]
XP_022933312.10.0e+0098.51receptor-like serine/threonine-protein kinase ALE2 [Cucurbita moschata][more]
XP_023531637.10.0e+0098.10receptor-like serine/threonine-protein kinase ALE2 [Cucurbita pepo subsp. pepo][more]
XP_022966870.10.0e+0096.20receptor-like serine/threonine-protein kinase ALE2 [Cucurbita maxima][more]
XP_023516974.10.0e+0076.97receptor-like serine/threonine-protein kinase ALE2 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q8RWW03.4e-16750.07Receptor-like serine/threonine-protein kinase ALE2 OS=Arabidopsis thaliana OX=37... [more]
Q9FE201.1e-8344.55Serine/threonine-protein kinase PBS1 OS=Arabidopsis thaliana OX=3702 GN=PBS1 PE=... [more]
Q6I5Q66.9e-8350.15Receptor-like cytoplasmic kinase 185 OS=Oryza sativa subsp. japonica OX=39947 GN... [more]
Q1PDV61.7e-8149.69Serine/threonine-protein kinase PBL27 OS=Arabidopsis thaliana OX=3702 GN=PBL27 P... [more]
F4JEQ28.4e-8151.88Probable serine/threonine-protein kinase PBL23 OS=Arabidopsis thaliana OX=3702 G... [more]
Match NameE-valueIdentityDescription
A0A6J1EZE70.0e+0098.51receptor-like serine/threonine-protein kinase ALE2 OS=Cucurbita moschata OX=3662... [more]
A0A6J1HSS70.0e+0096.20receptor-like serine/threonine-protein kinase ALE2 OS=Cucurbita maxima OX=3661 G... [more]
A0A5D3CEX40.0e+0076.99Receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo var. makuwa O... [more]
A0A1S4DYF00.0e+0076.85receptor-like serine/threonine-protein kinase ALE2 OS=Cucumis melo OX=3656 GN=LO... [more]
A0A6J1JEQ42.7e-31076.85receptor-like serine/threonine-protein kinase ALE2 OS=Cucurbita maxima OX=3661 G... [more]
Match NameE-valueIdentityDescription
AT5G56890.12.0e-22659.95Protein kinase superfamily protein [more]
AT2G20300.12.4e-16850.07Protein kinase superfamily protein [more]
AT4G02010.14.1e-9956.01Protein kinase superfamily protein [more]
AT5G13160.17.5e-8544.55Protein kinase superfamily protein [more]
AT5G18610.11.2e-8249.69Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 420..646
e-value: 3.9E-60
score: 204.7
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 320..419
e-value: 3.1E-33
score: 116.0
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 42..70
NoneNo IPR availablePANTHERPTHR47989OS01G0750732 PROTEINcoord: 39..729
NoneNo IPR availablePANTHERPTHR47989:SF9PROTEIN KINASE SUPERFAMILY PROTEINcoord: 39..729
NoneNo IPR availableCDDcd14066STKc_IRAKcoord: 350..618
e-value: 3.65489E-99
score: 304.966
IPR001245Serine-threonine/tyrosine-protein kinase, catalytic domainPFAMPF07714PK_Tyr_Ser-Thrcoord: 348..617
e-value: 1.5E-49
score: 168.7
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 350..372
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 466..478
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 344..620
score: 38.251564
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 323..617

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21960-RACarg21960-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004672 protein kinase activity