Carg21861 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg21861 vs. NCBI nr
Match: KAG7030054.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1528.1 bits (3955), Expect = 0.0e+00
Identity = 765/765 (100.00%), Postives = 765/765 (100.00%), Query Frame = 0

Query: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60
           MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG 120
           RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG 120

Query: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180
           NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240
           GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKA 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKA
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKA 300

Query: 301 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360
           TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH
Sbjct: 301 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360

Query: 361 GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV 420
           GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV
Sbjct: 361 GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV 420

Query: 421 ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC 480
           ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC
Sbjct: 421 ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC 480

Query: 481 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540
           QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI
Sbjct: 481 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540

Query: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600
           SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA
Sbjct: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600

Query: 601 IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS 660
           IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS
Sbjct: 601 IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS 660

Query: 661 KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL 720
           KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL
Sbjct: 661 KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL 720

Query: 721 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF
Sbjct: 721 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 765

BLAST of Carg21861 vs. NCBI nr
Match: KAG6599114.1 (Neutral ceramidase 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1525.8 bits (3949), Expect = 0.0e+00
Identity = 763/765 (99.74%), Postives = 765/765 (100.00%), Query Frame = 0

Query: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60
           MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF
Sbjct: 1   MRRQLLKVVATAVLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHF 60

Query: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG 120
           RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLT+LSKLKPRHFIQRFG
Sbjct: 61  RLRARTFIVAKSVDGPRIAFVNLDAGMASQLVTIKLLERLKASLTYLSKLKPRHFIQRFG 120

Query: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180
           NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP
Sbjct: 121 NLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKP 180

Query: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240
           GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW
Sbjct: 181 GFILINKGEVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSW 240

Query: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKA 300
           FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDI+ELMKKAETIKA
Sbjct: 241 FPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDILELMKKAETIKA 300

Query: 301 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360
           TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH
Sbjct: 301 TGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCH 360

Query: 361 GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV 420
           GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV
Sbjct: 361 GSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEV 420

Query: 421 ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC 480
           ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC
Sbjct: 421 ELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGC 480

Query: 481 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540
           QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI
Sbjct: 481 QKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLI 540

Query: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600
           SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA
Sbjct: 541 SNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLA 600

Query: 601 IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS 660
           IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS
Sbjct: 601 IAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGS 660

Query: 661 KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL 720
           KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL
Sbjct: 661 KQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSL 720

Query: 721 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF
Sbjct: 721 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 765

BLAST of Carg21861 vs. NCBI nr
Match: XP_038885883.1 (neutral ceramidase 2-like [Benincasa hispida])

HSP 1 Score: 1321.6 bits (3419), Expect = 0.0e+00
Identity = 662/753 (87.92%), Postives = 692/753 (91.90%), Query Frame = 0

Query: 13  VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKS 72
           V+AAVS C   +GDWLVGVGSFDMTGPAA VNM+GYANMDQ+ AGIHFRLRARTF+VAK+
Sbjct: 4   VIAAVSWC--VNGDWLVGVGSFDMTGPAAGVNMIGYANMDQNTAGIHFRLRARTFVVAKT 63

Query: 73  VDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISG 132
           VDGPRIAF+NLDAGMASQLVTIK+LERLK+               RFG+LYTEENVAISG
Sbjct: 64  VDGPRIAFINLDAGMASQLVTIKVLERLKS---------------RFGDLYTEENVAISG 123

Query: 133 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVEN 192
           IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLKPGFIL NKG+VEN
Sbjct: 124 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGDVEN 183

Query: 193 AGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDN 252
            GINRSPSAYLMNP EERARY  NVD EMS++K V+G SG S+GAFSWFPTHGTSMSRDN
Sbjct: 184 GGINRSPSAYLMNPEEERARYANNVDKEMSVLKFVDGESGNSIGAFSWFPTHGTSMSRDN 243

Query: 253 KLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQ 312
           KLISGDNKGAAARFFEDWV AS R+   +S+N S IVELM+KAETIKATGGKRCSKTSSQ
Sbjct: 244 KLISGDNKGAAARFFEDWV-ASKRTKITSSNNTSGIVELMRKAETIKATGGKRCSKTSSQ 303

Query: 313 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPG 372
           +SKVRKNDGSLFV AFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPG
Sbjct: 304 ASKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPG 363

Query: 373 FPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCP 432
           FPDEILSTKIIGERQF KA DLFTTATE+LTGEIDFRHVYLNFTDIEVEL G+NVVKTCP
Sbjct: 364 FPDEILSTKIIGERQFQKAADLFTTATEKLTGEIDFRHVYLNFTDIEVELDGNNVVKTCP 423

Query: 433 AALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGE 492
           AALGPGFAAGTTDGPGVFGFQQGDT+INKLWKKLRDSL+KPSEFQVGCQKPKTVLLDTGE
Sbjct: 424 AALGPGFAAGTTDGPGVFGFQQGDTEINKLWKKLRDSLKKPSEFQVGCQKPKTVLLDTGE 483

Query: 493 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 552
           MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGR+LREAVKETLI NGNGEFDDDTH
Sbjct: 484 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRQLREAVKETLIRNGNGEFDDDTH 543

Query: 553 VVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKP 612
           VVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAK E IAKP
Sbjct: 544 VVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKHENIAKP 603

Query: 613 IVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSAN 672
           I SPPDLSSVQLRFV DPF ESPPNG+NFGDIQQDIKLP  GWFKRG KQKP A FWSAN
Sbjct: 604 IASPPDLSSVQLRFVIDPFGESPPNGINFGDIQQDIKLPTNGWFKRGGKQKPAATFWSAN 663

Query: 673 PRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDAN 732
           PRFDLLTEGTYAVVERLEK+RWTPVYDDDDFSL FKW LDNNTF NS+ATIEWDIPIDA+
Sbjct: 664 PRFDLLTEGTYAVVERLEKQRWTPVYDDDDFSLFFKWTLDNNTFINSIATIEWDIPIDAD 723

Query: 733 PGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           PGVYRLRHFGSSKS INSTNIYFTGAS AFAVF
Sbjct: 724 PGVYRLRHFGSSKSTINSTNIYFTGASQAFAVF 738

BLAST of Carg21861 vs. NCBI nr
Match: XP_008464893.1 (PREDICTED: neutral ceramidase-like [Cucumis melo])

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 654/753 (86.85%), Postives = 693/753 (92.03%), Query Frame = 0

Query: 13  VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKS 72
           V+  VS C   +GDWLVGVGSFDMTGPAAEVNMMGYANMDQ  AGIHFRLRARTF+VA+S
Sbjct: 4   VVLDVSWC--VNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVARS 63

Query: 73  VDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISG 132
           +DGPRIAFVNLDAGMASQLVTIK+LERL+      S+      +QRFG+LYTEENVAISG
Sbjct: 64  IDGPRIAFVNLDAGMASQLVTIKVLERLQ------SRRNINISVQRFGDLYTEENVAISG 123

Query: 133 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVEN 192
           IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLKPGFIL NKGEV+N
Sbjct: 124 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDN 183

Query: 193 AGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDN 252
           AGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G SG S+GAFSWFPTHGTSMSRDN
Sbjct: 184 AGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDN 243

Query: 253 KLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQ 312
           KLISGDNKGAAARFFEDW A++ R+T   S+N S I ELMKKAE IKATGGK+CSKTSS+
Sbjct: 244 KLISGDNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSK 303

Query: 313 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPG 372
           SSKVRKNDGSLFV AFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPG
Sbjct: 304 SSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPG 363

Query: 373 FPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCP 432
           FPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE+ G+NVV+TCP
Sbjct: 364 FPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCP 423

Query: 433 AALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGE 492
           AALGPGFAAGTTDGPGVFGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGE
Sbjct: 424 AALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGE 483

Query: 493 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 552
           MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH
Sbjct: 484 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 543

Query: 553 VVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKP 612
           VVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGEK+AKP
Sbjct: 544 VVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKP 603

Query: 613 IVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSAN 672
           I SPPDLSSVQLR V DPF ESPP+GVNFGDIQQD++LPK GWFK GSKQKPTA FWSAN
Sbjct: 604 IASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSAN 663

Query: 673 PRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDAN 732
           PRFDLLTEGTYA+VERLEK+RWTP YDDDDFS+ FKW LDN T  NSLATIEWDIPIDA+
Sbjct: 664 PRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDAD 723

Query: 733 PGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           PGVYRLRHFGSS+S INSTNIYFTGAS AFAVF
Sbjct: 724 PGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of Carg21861 vs. NCBI nr
Match: XP_031742885.1 (neutral ceramidase 2 [Cucumis sativus])

HSP 1 Score: 1312.7 bits (3396), Expect = 0.0e+00
Identity = 653/753 (86.72%), Postives = 691/753 (91.77%), Query Frame = 0

Query: 13  VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKS 72
           V+ AVS C   +GDWLVGVGSFDMTGPAAEVNMMGYANMDQ+ AGIHFRLRARTF+VA S
Sbjct: 4   VVVAVSWC--VNGDWLVGVGSFDMTGPAAEVNMMGYANMDQNTAGIHFRLRARTFVVASS 63

Query: 73  VDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISG 132
           VDGPRIAFVNLDAGMASQLVTIK+LERLK+S+           +QRFG++YTEENVAISG
Sbjct: 64  VDGPRIAFVNLDAGMASQLVTIKVLERLKSSI----------LVQRFGDVYTEENVAISG 123

Query: 133 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVEN 192
           IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLKPGFIL NKGEVEN
Sbjct: 124 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVEN 183

Query: 193 AGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDN 252
           AGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G +G S+GAFSWFPTHGTSMSRDN
Sbjct: 184 AGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGENGNSIGAFSWFPTHGTSMSRDN 243

Query: 253 KLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQ 312
           KLISGDNKGAAARFFEDW A++ R+T   S+N S I +LMKKA  IKATGGKRCSKTSS+
Sbjct: 244 KLISGDNKGAAARFFEDWAASNRRTTTTPSNNTSGIEDLMKKAGKIKATGGKRCSKTSSK 303

Query: 313 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPG 372
           SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSG PCDFN SSCHGSDLLCVGRGPG
Sbjct: 304 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGNPCDFNHSSCHGSDLLCVGRGPG 363

Query: 373 FPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCP 432
           FPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEV + G+NVV+TCP
Sbjct: 364 FPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVAIDGNNVVRTCP 423

Query: 433 AALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGE 492
           AALGPGFAAGTTDGPG FGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGE
Sbjct: 424 AALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGE 483

Query: 493 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 552
           MFEPYAWAPAILPIQILRLG LIILSVPGEFTTMAGRRLREAVKETLISNGNG FDDDTH
Sbjct: 484 MFEPYAWAPAILPIQILRLGNLIILSVPGEFTTMAGRRLREAVKETLISNGNGVFDDDTH 543

Query: 553 VVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKP 612
           VVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKP
Sbjct: 544 VVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKP 603

Query: 613 IVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSAN 672
           I SPPDLSSVQLR V DPF ESPPNGVNFGDIQQD++LPK GWFK GSKQKPTA FWSAN
Sbjct: 604 IASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSAN 663

Query: 673 PRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDAN 732
           PRFDLLTEGTYA+VERLEK+RWTP YDDDDFSL FKW LD NTF N+L TIEWDIPIDA+
Sbjct: 664 PRFDLLTEGTYALVERLEKQRWTPAYDDDDFSLFFKWKLD-NTFINNLVTIEWDIPIDAD 723

Query: 733 PGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           PGVYRLRHFGSS+S INSTN+YFTGAS AFAVF
Sbjct: 724 PGVYRLRHFGSSRSTINSTNVYFTGASRAFAVF 743

BLAST of Carg21861 vs. ExPASy Swiss-Prot
Match: Q304B9 (Neutral ceramidase 2 OS=Arabidopsis thaliana OX=3702 GN=NCER2 PE=3 SV=1)

HSP 1 Score: 841.3 bits (2172), Expect = 8.9e-243
Identity = 443/754 (58.75%), Postives = 543/754 (72.02%), Query Frame = 0

Query: 27  WLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFVNLDAG 86
           +L+GVGS+D+TGPAA+VNMMGYAN DQ A+GIHFRLRAR FIVA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 87  MASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGGYLQYL 146
           MASQ+VTIK+LERLKA               R+G LYTE+NVAISGIHTHAGPGGYLQY+
Sbjct: 86  MASQIVTIKVLERLKA---------------RYGELYTEKNVAISGIHTHAGPGGYLQYV 145

Query: 147 VYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSAYLMNP 206
            Y +TSLGFV+QSFD +VN IEQSIVQAH SL+PG   +NKG++ +AG+NRSPS+YL NP
Sbjct: 146 TYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNP 205

Query: 207 PEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 266
             ER++Y  +VD EM+LVK V+   G + G+F+WF THGTSMSR N LISGDNKGAAARF
Sbjct: 206 AAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARF 265

Query: 267 FEDWV-------AASNRSTRATSSNKSDIV----ELMKKAETIKATGGKRCSKTSSQSSK 326
            EDW         +S    R  S+  SD       L+  A T K++ G    K+    ++
Sbjct: 266 MEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTR 325

Query: 327 VRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPD 386
           VR      FV AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+PD
Sbjct: 326 VRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPD 385

Query: 387 EILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTC 446
           E  ST+IIGE+QF  AV+LF  ATE+L G+I ++H YL+F++++V +    GG   VKTC
Sbjct: 386 EFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTC 445

Query: 447 PAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTG 506
           PAA+G GFAAGTTDGPG F F+QGD Q N  W+ +R+ LR P   QV CQKPK +LLDTG
Sbjct: 446 PAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTG 505

Query: 507 EMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDT 566
           EM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ 
Sbjct: 506 EMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNM 565

Query: 567 HVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAK 626
           HVVIAGLTN YSQY+AT EEYE QRYE ASTLYG HTL+AYIQEFKKLA A+  G  + +
Sbjct: 566 HVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPR 625

Query: 627 PIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSA 686
               PPDL   Q+  +S   ++S P GV FGD++ D+  P    F+RG  Q+  A FWS 
Sbjct: 626 G-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSG 685

Query: 687 NPRFDLLTEGTYAVVERL-EKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPID 746
            PR DL+TEG++AVVE L E  +W PVYDDDDFSL FKW+      + S ATIEW +P  
Sbjct: 686 CPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPES 745

Query: 747 ANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 765
           A  GVYR+RH+G+SKS   S +  F+G+SSAF V
Sbjct: 746 AVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVV 756

BLAST of Carg21861 vs. ExPASy Swiss-Prot
Match: Q0JL46 (Neutral ceramidase OS=Oryza sativa subsp. japonica OX=39947 GN=Os01g0624000 PE=1 SV=1)

HSP 1 Score: 827.0 bits (2135), Expect = 1.7e-238
Identity = 428/768 (55.73%), Postives = 550/768 (71.61%), Query Frame = 0

Query: 27  WLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFVNLDAG 86
           +LVG+GSFD+TGPAA+VNMMGYAN +Q A+GIHFRL++R FIVA+  +G R+ FVN+DA 
Sbjct: 41  YLVGMGSFDITGPAADVNMMGYANTEQIASGIHFRLKSRAFIVAEP-NGKRVVFVNIDAC 100

Query: 87  MASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGGYLQYL 146
           MASQ+VTIK+LERLKA               R+G+LY E NVAISGIHTHAGPGGYLQY+
Sbjct: 101 MASQIVTIKVLERLKA---------------RYGDLYNENNVAISGIHTHAGPGGYLQYV 160

Query: 147 VYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSAYLMNP 206
           VY +TSLGFV+QSFD IV+ IEQSIV+AH +L+PG I +NKG++ +AG+NRSPSAYL NP
Sbjct: 161 VYIVTSLGFVRQSFDVIVDGIEQSIVEAHNNLRPGKIFVNKGDLLDAGVNRSPSAYLNNP 220

Query: 207 PEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 266
            EER++Y  NVD EM+L+K V+   G  +G+F+WF THGTSMSR N LISGDNKGAAARF
Sbjct: 221 AEERSKYEYNVDKEMTLIKFVDDELG-PVGSFNWFATHGTSMSRTNSLISGDNKGAAARF 280

Query: 267 FEDWVAASNRSTRATSSNKSDI------------------------VELMKKAETIKATG 326
            EDW        ++  +N  D+                         +L++ A + +A+G
Sbjct: 281 MEDWAEQMGLPKQSAHANSDDLRSLHKTSVLPRRVSTIIPEPNEITDDLIQLASSYEASG 340

Query: 327 GKRCSKTS-SQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHG 386
           G+R + +S ++  +  + +   FV AFCQSN GDV+PNVLG FC D+  PCDFN S+C+G
Sbjct: 341 GRRLAGSSITRRIRSTQQNKPKFVSAFCQSNCGDVSPNVLGTFCIDTNLPCDFNHSTCNG 400

Query: 387 SDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVE 446
            + LC GRGPG+PDE  ST++IG RQFLKA DLF +A+E + G+ID+RH YL+F+ +EV+
Sbjct: 401 KNELCYGRGPGYPDEFESTRVIGNRQFLKARDLFDSASEEIQGKIDYRHTYLDFSKLEVK 460

Query: 447 L----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQ 506
           +    GG   VKTCPAA+G  FAAGTTDGPG F F+QGD + N  WK +R+ L+ P + Q
Sbjct: 461 VSTSAGGQQTVKTCPAAMGFAFAAGTTDGPGAFDFRQGDVKGNPFWKLVRNLLKTPGKDQ 520

Query: 507 VGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKE 566
           V C  PK +LLDTGEM EPY WAPAILP+Q++R+G+L+IL VPGEFTTMAGRRLR+AVK 
Sbjct: 521 VECHSPKPILLDTGEMKEPYDWAPAILPVQMIRIGQLVILCVPGEFTTMAGRRLRDAVK- 580

Query: 567 TLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFK 626
           T++++GN EFD + HVV+AGLTN+YSQY+ T EEY+ QRYE ASTLYGPHTLSAYIQEF+
Sbjct: 581 TVLTSGNSEFDKNIHVVLAGLTNSYSQYITTFEEYQIQRYEGASTLYGPHTLSAYIQEFQ 640

Query: 627 KLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFK 686
           KLA+A+   +++      PPD+   Q+  +     +S P GV FGD+  D+  P    F 
Sbjct: 641 KLAMAMIANKEVPTNF-QPPDMLDKQIGLLPGVVFDSTPLGVKFGDVNSDV--PGNSTFN 700

Query: 687 RGSKQKPTARFWSANPRFDLLTEGTYAVVERLE-KRRWTPVYDDDDFSLVFKWALDNNTF 746
           +GS    T  F+SA PR DLLT+GT+A+VE+L+    W PVYDDDD+SL FKW+      
Sbjct: 701 KGSTVNAT--FYSACPRNDLLTDGTFALVEKLDGNNNWVPVYDDDDWSLRFKWSRPARLS 760

Query: 747 TNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 765
           + S AT+EW +P DA  GVYRLRHFG+SK    S   +FTG S AFAV
Sbjct: 761 SRSFATLEWTVPEDAAAGVYRLRHFGASKPMFGSVR-HFTGTSRAFAV 784

BLAST of Carg21861 vs. ExPASy Swiss-Prot
Match: F4HQM3 (Neutral ceramidase 1 OS=Arabidopsis thaliana OX=3702 GN=NCER1 PE=2 SV=1)

HSP 1 Score: 807.4 bits (2084), Expect = 1.4e-232
Identity = 423/765 (55.29%), Postives = 544/765 (71.11%), Query Frame = 0

Query: 26  DWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFVNLDA 85
           ++L+G+GS+D+TGPAA+VNMMGYANM+Q A+GIHFRLRARTFIV++   G R+ FVNLDA
Sbjct: 36  EYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNLDA 95

Query: 86  GMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGGYLQY 145
            MASQ+V +K++ERLKA               R+G+LYTE+NV ISGIHTHAGPGGYLQY
Sbjct: 96  CMASQIVKLKVIERLKA---------------RYGDLYTEQNVGISGIHTHAGPGGYLQY 155

Query: 146 LVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSAYLMN 205
           +VY +TSLGFV+QSFDA+V+ IE SI+QAH +L+PG I +N GE+ +AG+NRSPSAYL N
Sbjct: 156 VVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNN 215

Query: 206 PPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAAR 265
           P +ER+++  NVD EM+L+K V+   G  +G+F+WF THGTSMSR N LISGDNKGAA+R
Sbjct: 216 PSKERSKHKYNVDKEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASR 275

Query: 266 FFEDWVAASNRSTRATSSNKSDIV----------------ELMKKAETIKATGGKRCSKT 325
           F EDW   +      +    SD +                EL++ A   ++  GK  ++ 
Sbjct: 276 FMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRI 335

Query: 326 SSQSSKV----RKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLL 385
           SS + +V    RK D   FV AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +
Sbjct: 336 SSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEM 395

Query: 386 CVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL--- 445
           C GRGPG+PDE  ST+IIGERQF  A++LF  A+E+L G++D+RHVY++F+ + V L   
Sbjct: 396 CYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKK 455

Query: 446 -GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQ 505
            G   VVKTCPAA+G  FAAGTTDGPG F F QGD + N  W+ +R+ L+ P + Q+ C 
Sbjct: 456 DGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCH 515

Query: 506 KPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS 565
            PK +LLDTGEM +PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L +
Sbjct: 516 YPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKN 575

Query: 566 NGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAI 625
           +GN +   + HVVIAGL N YSQYV T EEY+ QRYE ASTLYGPHTLS YIQEFKKL+ 
Sbjct: 576 SGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSK 635

Query: 626 AIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSK 685
           ++     + +P   PPDL   QL F++   +++ P+G +FGD+  D+  PK    KRG+ 
Sbjct: 636 SLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNG 695

Query: 686 QKPTARFWSANPRFDLLTEGTYAVVERLEK--RRWTPVYDDDDFSLVFKWALDNNTFTNS 745
           Q  T  F SA PR DLLTEGT+ +VERLE+  + WTPVYDDDD  L FKW+      + S
Sbjct: 696 Q-VTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRS 755

Query: 746 LATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 765
            AT+EW IP  A+PGVYR+ HFG++K    S + +FTG+SSAF V
Sbjct: 756 QATVEWRIPESASPGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of Carg21861 vs. ExPASy Swiss-Prot
Match: F4KHQ8 (Neutral ceramidase 3 OS=Arabidopsis thaliana OX=3702 GN=NCER3 PE=2 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 2.0e-226
Identity = 425/765 (55.56%), Postives = 532/765 (69.54%), Query Frame = 0

Query: 20  CCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIA 79
           C  +D D+L+G+GS+D+TGPAA+VNMMGYANM+Q A+G+HFRLRAR FIVA+     RIA
Sbjct: 22  CIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIA 81

Query: 80  FVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGP 139
           FVNLDAGMASQLVTIK++ERLK               QR+G LYTEENVAISG HTHAGP
Sbjct: 82  FVNLDAGMASQLVTIKVIERLK---------------QRYGELYTEENVAISGTHTHAGP 141

Query: 140 GGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSP 199
           GGYLQY++Y +TSLGFV QSF+A+V+ IEQSI+QAH +L+PG ILINKGE+ +AG+NRSP
Sbjct: 142 GGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSP 201

Query: 200 SAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDN 259
           SAYL NP  ER++Y  +VD EM+LVK V+                             D 
Sbjct: 202 SAYLNNPAHERSKYEYDVDKEMTLVKFVD-----------------------------DQ 261

Query: 260 KGAAARFFEDWVAASN-------RSTRATSSNKSDIV--ELMKKAETIKATGGKRCSKTS 319
            G  AR  EDW    N        S R  SS  SD    +LM+ A ++ +TGGK  ++ S
Sbjct: 262 WGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTVTRMS 321

Query: 320 SQSSKVRKN----DGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLC 379
           S + +VR      D   FV AFCQ+N GDV+PNVLGAFC D+G PC+FN+S+C G +  C
Sbjct: 322 SVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQC 381

Query: 380 VGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL---- 439
            GRGPG+PDE  ST+IIGERQF KA DLFT A+E + G++D+RH Y++F+ +EV +    
Sbjct: 382 YGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQN 441

Query: 440 GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQK 499
           GG  VVKTCPAA+G GFAAGTTDGPG F F+QGD Q N  W+ +R+ L+ P+E QV CQ+
Sbjct: 442 GGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQR 501

Query: 500 PKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISN 559
           PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  L   
Sbjct: 502 PKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEG 561

Query: 560 GNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIA 619
            NG    +  VVIAGLTN+YSQY+AT EEY+ QRYE ASTLYGPHTLS YIQEFKKLA  
Sbjct: 562 SNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLAND 621

Query: 620 IAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQ 679
           +   +    P   PPDL   Q+  ++    +  P G  FGD+  D+  P++  F++G+  
Sbjct: 622 LLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKGA-D 681

Query: 680 KPTARFWSANPRFDLLTEGTYAVVER-LEKRR-WTPVYDDDDFSLVFKWALDNNTFTNSL 739
               +F SANPR DL+TEGT+A+VER LE R  W PVYDDDDF L FKW+      T S 
Sbjct: 682 IVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQST 733

Query: 740 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           ATIEW IP  A+PGVYR+ HFGS+K+ I+S + +F+G+SSAF V+
Sbjct: 742 ATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVVY 733

BLAST of Carg21861 vs. ExPASy Swiss-Prot
Match: Q55G11 (Neutral ceramidase B OS=Dictyostelium discoideum OX=44689 GN=dcd2B PE=3 SV=1)

HSP 1 Score: 516.2 bits (1328), Expect = 6.5e-145
Identity = 311/754 (41.25%), Postives = 432/754 (57.29%), Query Frame = 0

Query: 22  AADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFV 81
           + D  + +G G +D+TG +AEVN+MGYAN  Q  AGIHFR RAR F+   S +G R  +V
Sbjct: 41  SGDSSYQIGAGIYDITGASAEVNLMGYANPLQVGAGIHFRQRARAFVFVDS-NGNRAVYV 100

Query: 82  NLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFG-NLYTEENVAISGIHTHAGPG 141
           + D+ M  Q V I ++E L+                 FG N+YTE NV +SG HTH+GP 
Sbjct: 101 STDSCMIFQEVKIHVVELLQ---------------DIFGPNVYTEANVLLSGTHTHSGPA 160

Query: 142 GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPS 201
           G+ QY +Y ITSLGF +++FD I N I Q+IV+AH S++P  +    GE+ N  INRSP 
Sbjct: 161 GFSQYALYGITSLGFYKKNFDTICNGIVQAIVKAHKSVQPANMFTETGELWNTNINRSPF 220

Query: 202 AYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNK 261
           AY  NP EE+A Y +NVD  M++++ +E  +G    A S+F  H TSM+  N LISGDNK
Sbjct: 221 AYDNNPEEEKAMYDSNVDKNMTVLR-IEDMNGNPFAAISFFAVHCTSMNNTNHLISGDNK 280

Query: 262 GAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKND 321
           G A+  +E  V                             T GK                
Sbjct: 281 GYASYLWEKQVNG-------------------------PGTAGK---------------- 340

Query: 322 GSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIL-S 381
              FV AF QSN GDV+PN  G  C D G PCD+  S+C+G +  C   GPG   ++  S
Sbjct: 341 -GPFVAAFGQSNEGDVSPNTRGPTCRD-GSPCDYKTSTCNGRNEECWSLGPGKDGDMFES 400

Query: 382 TKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVE----LGGDNVVKTCPAAL 441
           T+IIG  QF KA++LF  A+ +++G + +RH ++ FT++ VE     G DN   TC  A+
Sbjct: 401 TQIIGGNQFNKALELFNNASIQVSGPVQYRHSWVQFTNVSVEPPYNSGVDNAT-TCRGAM 460

Query: 442 GPGFAAGTTDGPGVFGFQQGD--TQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEM 501
           G  FAAGTTDGPG F F Q D  T  N  W  + D + KP+  Q+ CQ PK +LLD G M
Sbjct: 461 GYSFAAGTTDGPGAFNFVQSDNNTSGNPFWNFIGDFIAKPTPDQIRCQSPKPILLDVG-M 520

Query: 502 FEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHV 561
            EP  W P ++PIQI+ +G++++++VPGEFTTM+GRRLR +V+E +     GE  ++  V
Sbjct: 521 VEPIPWVPDVMPIQIVTIGQIVLVAVPGEFTTMSGRRLRNSVREII-----GESIENPIV 580

Query: 562 VIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKI-AKP 621
           +IAGL+N YS Y+AT EEY+ QRYE AST++GPHTL +Y+QEF KLA +I  G  + A P
Sbjct: 581 LIAGLSNTYSGYIATFEEYQVQRYEGASTVFGPHTLGSYMQEFGKLAQSIVDGTTVPAGP 640

Query: 622 IVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSAN 681
             +P +L+   L F+    +++ P+  +FG++  D+ L           +  +  F+  N
Sbjct: 641 --TPRNLTGHTLFFLPPVIVDAAPDFDDFGEVSIDVNL------NYSVNETVSCVFYGGN 700

Query: 682 PRFDLLTEGTYAVVERLE-KRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDA 741
           PR D + E ++  V+ L    +WT V DD D+   FKW + +  F  SL TIEW I  D 
Sbjct: 701 PRNDFMIESSFLSVDLLTGTDQWTTVLDDGDWDTKFKWKMHDLGF--SLITIEWVIAPDT 717

Query: 742 NPGVYRLRHFGSSKSAINSTNIY-FTGASSAFAV 765
            PG YR+ H G +K    S+N+  + G S  F V
Sbjct: 761 TPGTYRITHSGFAKKNPFSSNLTPYQGISRNFVV 717

BLAST of Carg21861 vs. ExPASy TrEMBL
Match: A0A1S3CMI6 (Neutral ceramidase OS=Cucumis melo OX=3656 GN=LOC103502652 PE=3 SV=1)

HSP 1 Score: 1314.7 bits (3401), Expect = 0.0e+00
Identity = 654/753 (86.85%), Postives = 693/753 (92.03%), Query Frame = 0

Query: 13  VLAAVSCCCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKS 72
           V+  VS C   +GDWLVGVGSFDMTGPAAEVNMMGYANMDQ  AGIHFRLRARTF+VA+S
Sbjct: 4   VVLDVSWC--VNGDWLVGVGSFDMTGPAAEVNMMGYANMDQVTAGIHFRLRARTFVVARS 63

Query: 73  VDGPRIAFVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISG 132
           +DGPRIAFVNLDAGMASQLVTIK+LERL+      S+      +QRFG+LYTEENVAISG
Sbjct: 64  IDGPRIAFVNLDAGMASQLVTIKVLERLQ------SRRNINISVQRFGDLYTEENVAISG 123

Query: 133 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVEN 192
           IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH SLKPGFIL NKGEV+N
Sbjct: 124 IHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAHESLKPGFILFNKGEVDN 183

Query: 193 AGINRSPSAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDN 252
           AGINRSPSAYLMNP EERA+Y  NVD EMS++K V+G SG S+GAFSWFPTHGTSMSRDN
Sbjct: 184 AGINRSPSAYLMNPEEERAKYANNVDKEMSILKFVDGESGNSIGAFSWFPTHGTSMSRDN 243

Query: 253 KLISGDNKGAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQ 312
           KLISGDNKGAAARFFEDW A++ R+T   S+N S I ELMKKAE IKATGGK+CSKTSS+
Sbjct: 244 KLISGDNKGAAARFFEDWAASNGRTTTTPSNNASVIEELMKKAEKIKATGGKQCSKTSSK 303

Query: 313 SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPG 372
           SSKVRKNDGSLFV AFCQSNVGDVTPNVLGAFC DSGKPCDFNRSSCHGSDLLCVGRGPG
Sbjct: 304 SSKVRKNDGSLFVAAFCQSNVGDVTPNVLGAFCIDSGKPCDFNRSSCHGSDLLCVGRGPG 363

Query: 373 FPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCP 432
           FPDEILSTKIIGERQFLKA DLFTTATE+LTGEIDFRHVYLNFTDIEVE+ G+NVV+TCP
Sbjct: 364 FPDEILSTKIIGERQFLKAADLFTTATEKLTGEIDFRHVYLNFTDIEVEIDGNNVVRTCP 423

Query: 433 AALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGE 492
           AALGPGFAAGTTDGPGVFGFQQGDT+INKLWK+LRDSL+KPSEFQVGCQKPKTVLLDTGE
Sbjct: 424 AALGPGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDSLKKPSEFQVGCQKPKTVLLDTGE 483

Query: 493 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 552
           MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH
Sbjct: 484 MFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTH 543

Query: 553 VVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKP 612
           VVIAGLTN YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEFKKLA AIAKGEK+AKP
Sbjct: 544 VVIAGLTNTYSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFKKLASAIAKGEKVAKP 603

Query: 613 IVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSAN 672
           I SPPDLSSVQLR V DPF ESPP+GVNFGDIQQD++LPK GWFK GSKQKPTA FWSAN
Sbjct: 604 IASPPDLSSVQLRLVMDPFGESPPDGVNFGDIQQDVELPKDGWFKTGSKQKPTATFWSAN 663

Query: 673 PRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDAN 732
           PRFDLLTEGTYA+VERLEK+RWTP YDDDDFS+ FKW LDN T  NSLATIEWDIPIDA+
Sbjct: 664 PRFDLLTEGTYALVERLEKQRWTPAYDDDDFSVSFKWKLDNTTLINSLATIEWDIPIDAD 723

Query: 733 PGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           PGVYRLRHFGSS+S INSTNIYFTGAS AFAVF
Sbjct: 724 PGVYRLRHFGSSRSTINSTNIYFTGASRAFAVF 748

BLAST of Carg21861 vs. ExPASy TrEMBL
Match: A0A0A0KII3 (Neutral ceramidase OS=Cucumis sativus OX=3659 GN=Csa_6G361230 PE=3 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 573/660 (86.82%), Postives = 605/660 (91.67%), Query Frame = 0

Query: 116 IQRFGNLYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH 175
           ++ FG++YTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSI+QAH
Sbjct: 1   MKTFGDVYTEENVAISGIHTHAGPGGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIIQAH 60

Query: 176 GSLKPGFILINKG----------EVENAGINRSPSAYLMNPPEERARYPTNVDTEMSLVK 235
            SLKPGFIL NKG          EVENAGINRSPSAYLMNP EERA+Y  NVD EMS++K
Sbjct: 61  ESLKPGFILFNKGKQIRQLFHFWEVENAGINRSPSAYLMNPEEERAKYANNVDKEMSILK 120

Query: 236 VVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWVAASNRSTRATSSNK 295
            V+G +G S+GAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDW A++ R+T   S+N 
Sbjct: 121 FVDGENGNSIGAFSWFPTHGTSMSRDNKLISGDNKGAAARFFEDWAASNRRTTTTPSNNT 180

Query: 296 SDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC 355
           S I +LMKKA  IKATGGKRCSKTSS+SSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC
Sbjct: 181 SGIEDLMKKAGKIKATGGKRCSKTSSKSSKVRKNDGSLFVGAFCQSNVGDVTPNVLGAFC 240

Query: 356 TDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGE 415
           TDSG PCDFN SSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKA DLFTTATE+LTGE
Sbjct: 241 TDSGNPCDFNHSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAADLFTTATEKLTGE 300

Query: 416 IDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKK 475
           IDFRHVYLNFTDIEV + G+NVV+TCPAALGPGFAAGTTDGPG FGFQQGDT+INKLWK+
Sbjct: 301 IDFRHVYLNFTDIEVAIDGNNVVRTCPAALGPGFAAGTTDGPGAFGFQQGDTEINKLWKQ 360

Query: 476 LRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTT 535
           LRDSL+KPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLG LIILSVPGEFTT
Sbjct: 361 LRDSLKKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGNLIILSVPGEFTT 420

Query: 536 MAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYG 595
           MAGRRLREAVKETLISNGNG FDDDTHVVIAGLTN YSQYVAT EEYEQQRYEAASTLYG
Sbjct: 421 MAGRRLREAVKETLISNGNGVFDDDTHVVIAGLTNTYSQYVATFEEYEQQRYEAASTLYG 480

Query: 596 PHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQ 655
           PHTLSAYIQEFKKLAIAIAKGEKIAKPI SPPDLSSVQLR V DPF ESPPNGVNFGDIQ
Sbjct: 481 PHTLSAYIQEFKKLAIAIAKGEKIAKPIASPPDLSSVQLRLVMDPFGESPPNGVNFGDIQ 540

Query: 656 QDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERLEKRRWTPVYDDDDFSL 715
           QD++LPK GWFK GSKQKPTA FWSANPRFDLLTEGTYA+VERLEK+RWTP YDDDDFSL
Sbjct: 541 QDVELPKDGWFKTGSKQKPTATFWSANPRFDLLTEGTYALVERLEKQRWTPAYDDDDFSL 600

Query: 716 VFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
            FKW LD NTF N+L TIEWDIPIDA+PGVYRLRHFGSS+S INSTN+YFTGAS AFAVF
Sbjct: 601 FFKWKLD-NTFINNLVTIEWDIPIDADPGVYRLRHFGSSRSTINSTNVYFTGASRAFAVF 659

BLAST of Carg21861 vs. ExPASy TrEMBL
Match: A0A2I4G350 (Neutral ceramidase OS=Juglans regia OX=51240 GN=LOC109004273 PE=3 SV=1)

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 564/743 (75.91%), Postives = 640/743 (86.14%), Query Frame = 0

Query: 22  AADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFV 81
           A +G++L GVGSFDMTGPAA VNMMGYAN+ Q+ AG+HFRLR RTFIVA+S  GPR AFV
Sbjct: 24  ATNGEYLTGVGSFDMTGPAAGVNMMGYANIFQNTAGVHFRLRTRTFIVAESPQGPRFAFV 83

Query: 82  NLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGG 141
           NLDAGMASQLVT K+LERLK+               RFGNLYT+ENVAISGIHTHAGPGG
Sbjct: 84  NLDAGMASQLVTTKVLERLKS---------------RFGNLYTQENVAISGIHTHAGPGG 143

Query: 142 YLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSA 201
           YLQYL+YSITS GFVQQSFDAIV AIEQSIVQAH +LKPG + INKGEVENAGINRSPSA
Sbjct: 144 YLQYLLYSITSAGFVQQSFDAIVGAIEQSIVQAHNNLKPGSVFINKGEVENAGINRSPSA 203

Query: 202 YLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKG 261
           YL NP EER RYP NVDT+M+L+K+++ GSG S+GAFSW+ THGTSMSRDNKLISGDNKG
Sbjct: 204 YLFNPAEERDRYPNNVDTQMTLLKLLDRGSGKSIGAFSWYATHGTSMSRDNKLISGDNKG 263

Query: 262 AAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKNDG 321
           AAARFFEDW  + N+S+    S+ ++I  L++KA+TIKATGG+ C KT+SQ+ KVRKNDG
Sbjct: 264 AAARFFEDWF-SFNKSSSTPISSPAEIETLVEKAQTIKATGGQPCGKTTSQAFKVRKNDG 323

Query: 322 SLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTK 381
           SLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHG+DLLCVGRGPG+PDEI STK
Sbjct: 324 SLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGNDLLCVGRGPGYPDEIQSTK 383

Query: 382 IIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELGGDNVVKTCPAALGPGFAA 441
           IIGERQF KAVDLF TATE LTG+ID+RHVYLNFT+I+VEL G  VVKTCPAA+GPGFAA
Sbjct: 384 IIGERQFQKAVDLFATATEELTGKIDYRHVYLNFTNIDVELDGSKVVKTCPAAVGPGFAA 443

Query: 442 GTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAP 501
           GTTDGPGVFGFQQGDT+I+++WKKLR++L++PS +QV CQKPKTVLLD GEMF+PYAWAP
Sbjct: 444 GTTDGPGVFGFQQGDTEISEMWKKLRNALKEPSPYQVECQKPKTVLLDAGEMFDPYAWAP 503

Query: 502 AILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNA 561
           AILPIQ+LRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDD T VVIAGLTN 
Sbjct: 504 AILPIQMLRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDKTEVVIAGLTNT 563

Query: 562 YSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSS 621
           YSQYVAT EEYEQQRYEAASTLYGPHTLSAYIQEF KLA A+AKGEK+     +PPDLSS
Sbjct: 564 YSQYVATFEEYEQQRYEAASTLYGPHTLSAYIQEFNKLAKAMAKGEKLISNGPTPPDLSS 623

Query: 622 VQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEG 681
           VQL+F+ +P  +SPP G NFGD++QDI +PK G F++G   KP+A FWSANPR+DLLTEG
Sbjct: 624 VQLKFLLEPVGDSPPPGKNFGDMKQDIIIPKSGTFRKG--DKPSATFWSANPRYDLLTEG 683

Query: 682 TYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHF 741
           T+AVVE L+  RW PV+DDDDF L FKW++DN++F   LATIEW+IP   + GVYRLRHF
Sbjct: 684 TFAVVEMLQVERWIPVFDDDDFCLYFKWSVDNSSF-YGLATIEWEIPEVVSSGVYRLRHF 743

Query: 742 GSSKSAINSTNIYFTGASSAFAV 765
           GS+K    S N YFTGAS+AF V
Sbjct: 744 GSAKKTKASPNTYFTGASTAFTV 747

BLAST of Carg21861 vs. ExPASy TrEMBL
Match: A0A2H5PA29 (Neutral ceramidase OS=Citrus unshiu OX=55188 GN=CUMW_117460 PE=3 SV=1)

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 565/748 (75.53%), Postives = 641/748 (85.70%), Query Frame = 0

Query: 22  AADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFV 81
           +ADG +LVGVGS+DMTGPAA +NMMGYAN+ Q+ AGIHFRLRARTFIVA+S +G R AFV
Sbjct: 112 SADGQYLVGVGSYDMTGPAAGINMMGYANLFQNTAGIHFRLRARTFIVAESSNGTRFAFV 171

Query: 82  NLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGG 141
           NLDAGMASQLVTIK+LE+LK                RFGNLYTEEN+AISGIHTHAGPGG
Sbjct: 172 NLDAGMASQLVTIKVLEKLKT---------------RFGNLYTEENIAISGIHTHAGPGG 231

Query: 142 YLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSA 201
           YLQYL+YSITSLGFVQQSFDAIV+AI QSIVQAH +LKPG I I KG+VENAGINRSPSA
Sbjct: 232 YLQYLIYSITSLGFVQQSFDAIVSAIVQSIVQAHNNLKPGSIFIRKGDVENAGINRSPSA 291

Query: 202 YLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKG 261
           YL+NP EERARYP NVD EM+L+K V+  SG S+GAF+W+ THGTSMSRDN LISGDNKG
Sbjct: 292 YLLNPAEERARYPENVDKEMTLLKFVDAASGKSIGAFNWYATHGTSMSRDNLLISGDNKG 351

Query: 262 AAARFFEDWVAASNRSTRATS---SNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRK 321
           AAARFFEDW ++ N S+  T+   S  SDI +LM+K++TIKATGG++C KT+SQ  KVRK
Sbjct: 352 AAARFFEDWFSSKNESSSITTSKFSTSSDIGKLMEKSKTIKATGGQQCGKTTSQGFKVRK 411

Query: 322 NDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEIL 381
           NDGSLFVGAFCQSNVGDVTPNVLGAFC DSGKPCDFN SSCHG D LC+GRGPG+PDEIL
Sbjct: 412 NDGSLFVGAFCQSNVGDVTPNVLGAFCIDSGKPCDFNHSSCHGDDQLCIGRGPGYPDEIL 471

Query: 382 STKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVELG--GDNVVKTCPAALG 441
           STKIIGERQF KAVDLF +AT++L+G+ID+RHVY+NFT+IEV L   G+N VKTCPAALG
Sbjct: 472 STKIIGERQFQKAVDLFNSATDQLSGKIDYRHVYINFTNIEVGLDGTGNNTVKTCPAALG 531

Query: 442 PGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEP 501
           PGFAAGTTDGPGVFGFQQGDT+INKLWK+LRD L+KPS++Q  CQKPK VLL +GEMFEP
Sbjct: 532 PGFAAGTTDGPGVFGFQQGDTEINKLWKQLRDVLKKPSQYQEECQKPKAVLLSSGEMFEP 591

Query: 502 YAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIA 561
           YAWAPA+LPIQILRLGKLIILSVPGEFTTMAGRRLREAVK+TLI+NGNGEFD+DTH+VIA
Sbjct: 592 YAWAPAVLPIQILRLGKLIILSVPGEFTTMAGRRLREAVKDTLINNGNGEFDNDTHIVIA 651

Query: 562 GLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSP 621
           GLTNAYSQY+AT EEY  QRYEAASTLYGPHTL AYIQEFKKLA A+AKGEK  K   SP
Sbjct: 652 GLTNAYSQYIATFEEYAHQRYEAASTLYGPHTLPAYIQEFKKLAQAMAKGEKTTKG-PSP 711

Query: 622 PDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFD 681
           PDLSSVQL    +P  +SPP G  FGDI++DI  PK G F +G   +P+A FWSANPR+D
Sbjct: 712 PDLSSVQLSLSLNPTGDSPPAGKKFGDIKEDINQPKDGSFNKG--DRPSATFWSANPRYD 771

Query: 682 LLTEGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVY 741
           LLTEGTYAVVE L+ +RW PVYDDDDFSL FKW+L+N +F + LAT+EW++P +A PGVY
Sbjct: 772 LLTEGTYAVVEMLQGKRWIPVYDDDDFSLYFKWSLENTSF-HGLATVEWEVPTEAIPGVY 831

Query: 742 RLRHFGSSKSAINSTNIYFTGASSAFAV 765
           RLRHFGSSK A+NSTN YFTGASSAF V
Sbjct: 832 RLRHFGSSKKAVNSTNHYFTGASSAFTV 840

BLAST of Carg21861 vs. ExPASy TrEMBL
Match: A0A251R941 (Neutral ceramidase OS=Prunus persica OX=3760 GN=PRUPE_1G356500 PE=3 SV=1)

HSP 1 Score: 1126.3 bits (2912), Expect = 0.0e+00
Identity = 564/745 (75.70%), Postives = 641/745 (86.04%), Query Frame = 0

Query: 21  CAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAF 80
           C  +G +L+GVGS+DMTGPAAEVNMMGYANMDQS AG+HFRLRARTFIVA+S  GPR AF
Sbjct: 32  CNGEG-YLIGVGSYDMTGPAAEVNMMGYANMDQSTAGVHFRLRARTFIVAESSQGPRFAF 91

Query: 81  VNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPG 140
           VNLDAGMASQLV IK+L++LK+               RFG+LYTEENVAISGIHTHAGPG
Sbjct: 92  VNLDAGMASQLVNIKVLDKLKS---------------RFGDLYTEENVAISGIHTHAGPG 151

Query: 141 GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPS 200
           GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAH +LKPG + IN+G+V NAGINRSPS
Sbjct: 152 GYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHHNLKPGSVFINEGDVVNAGINRSPS 211

Query: 201 AYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNK 260
           AYL+NP EERARYP+NVDT M+L+K V+  SG S+GAFSWF THGTSMS++N LISGDNK
Sbjct: 212 AYLLNPAEERARYPSNVDTLMTLLKFVDDASGKSVGAFSWFATHGTSMSKNNLLISGDNK 271

Query: 261 GAAARFFEDWVAASNRSTRATSSNKSDIVELMKKAETIKATGGKRCSKTSSQSSKVRKND 320
           GAAARFFEDW ++S+ S+  ++ + + ++ L+KKA+++KATGGK C KTSS+ SKVRKN+
Sbjct: 272 GAAARFFEDWFSSSSSSSTTSADSSASLLSLIKKAQSMKATGGKLCGKTSSRESKVRKNN 331

Query: 321 GSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILST 380
           GSLFVGAFCQSNVGDVTPNVLGAFC DSG+PCDFN SSCHG+DLLC+GRGPG+PDEILST
Sbjct: 332 GSLFVGAFCQSNVGDVTPNVLGAFCADSGRPCDFNHSSCHGNDLLCLGRGPGYPDEILST 391

Query: 381 KIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVE-LGGDNVVKTCPAALGPGF 440
           KIIGERQF KA  LFTTAT++LTG ID+RHVYLNFTDIEVE L G+  VKTCPAALGPGF
Sbjct: 392 KIIGERQFQKAAHLFTTATQQLTGTIDYRHVYLNFTDIEVELLDGNKKVKTCPAALGPGF 451

Query: 441 AAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAW 500
           AAGTTDGPG FGFQQGDT+INKLW+KLRD+L+KP+ +QV CQKPKTVLLDTGEMF PYAW
Sbjct: 452 AAGTTDGPGAFGFQQGDTKINKLWRKLRDALKKPTPYQVDCQKPKTVLLDTGEMFVPYAW 511

Query: 501 APAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLT 560
           APAILPIQ+LRLGKLIILSVPGEFTTMAGRRLREAVKETLISN NGEFDD+TH+VIAGLT
Sbjct: 512 APAILPIQMLRLGKLIILSVPGEFTTMAGRRLREAVKETLISNSNGEFDDETHIVIAGLT 571

Query: 561 NAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDL 620
           N YSQY+AT EEY QQRYEAASTLYGPHTLSAYIQEF+KLA A+AKGE+I K   SPPDL
Sbjct: 572 NTYSQYIATFEEYAQQRYEAASTLYGPHTLSAYIQEFEKLAKAMAKGEQITKG-PSPPDL 631

Query: 621 SSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLT 680
           SSVQLR + +P  + PP   NFGD++QDI +PK G FK+G   +P A FWSANPR+DLLT
Sbjct: 632 SSVQLRLLLEPTGDLPPPHTNFGDMKQDIIIPKSGSFKKG--DRPIATFWSANPRYDLLT 691

Query: 681 EGTYAVVERLEKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLR 740
           EGT+AVVE L+ + W PVYDDDDF L FKW +DN T   + ATIEW+IP DA+ GVYRLR
Sbjct: 692 EGTFAVVELLQGKHWVPVYDDDDFCLFFKWKVDNITLYGT-ATIEWEIPRDADSGVYRLR 751

Query: 741 HFGSSKSAINSTNIYFTGASSAFAV 765
           HFGSSK   +S NIYFTGASS FAV
Sbjct: 752 HFGSSKKTKDSPNIYFTGASSGFAV 756

BLAST of Carg21861 vs. TAIR 10
Match: AT2G38010.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 841.3 bits (2172), Expect = 6.3e-244
Identity = 443/754 (58.75%), Postives = 543/754 (72.02%), Query Frame = 0

Query: 27  WLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFVNLDAG 86
           +L+GVGS+D+TGPAA+VNMMGYAN DQ A+GIHFRLRAR FIVA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 87  MASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGGYLQYL 146
           MASQ+VTIK+LERLKA               R+G LYTE+NVAISGIHTHAGPGGYLQY+
Sbjct: 86  MASQIVTIKVLERLKA---------------RYGELYTEKNVAISGIHTHAGPGGYLQYV 145

Query: 147 VYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSAYLMNP 206
            Y +TSLGFV+QSFD +VN IEQSIVQAH SL+PG   +NKG++ +AG+NRSPS+YL NP
Sbjct: 146 TYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNP 205

Query: 207 PEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 266
             ER++Y  +VD EM+LVK V+   G + G+F+WF THGTSMSR N LISGDNKGAAARF
Sbjct: 206 AAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARF 265

Query: 267 FEDWV-------AASNRSTRATSSNKSDIV----ELMKKAETIKATGGKRCSKTSSQSSK 326
            EDW         +S    R  S+  SD       L+  A T K++ G    K+    ++
Sbjct: 266 MEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRDRLLDIAATYKSSRGHSVDKSLDVKTR 325

Query: 327 VRKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPD 386
           VR      FV AFCQSN GDV+PN LG FC D+G PCDFN S+C+G + LC GRGPG+PD
Sbjct: 326 VRNGSKRKFVSAFCQSNCGDVSPNTLGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPD 385

Query: 387 EILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTC 446
           E  ST+IIGE+QF  AV+LF  ATE+L G+I ++H YL+F++++V +    GG   VKTC
Sbjct: 386 EFESTRIIGEKQFKMAVELFNKATEKLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTC 445

Query: 447 PAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTG 506
           PAA+G GFAAGTTDGPG F F+QGD Q N  W+ +R+ LR P   QV CQKPK +LLDTG
Sbjct: 446 PAAMGFGFAAGTTDGPGAFDFKQGDDQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTG 505

Query: 507 EMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDT 566
           EM EPY WAP+ILPIQILR+G+L+ILSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ 
Sbjct: 506 EMKEPYDWAPSILPIQILRIGQLVILSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNM 565

Query: 567 HVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAK 626
           HVVIAGLTN YSQY+AT EEYE QRYE ASTLYG HTL+AYIQEFKKLA A+  G  + +
Sbjct: 566 HVVIAGLTNTYSQYIATFEEYEVQRYEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPR 625

Query: 627 PIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSA 686
               PPDL   Q+  +S   ++S P GV FGD++ D+  P    F+RG  Q+  A FWS 
Sbjct: 626 G-PQPPDLLDKQISLLSPVVVDSTPLGVKFGDVKADV--PPKSTFRRG--QQVNATFWSG 685

Query: 687 NPRFDLLTEGTYAVVERL-EKRRWTPVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPID 746
            PR DL+TEG++AVVE L E  +W PVYDDDDFSL FKW+      + S ATIEW +P  
Sbjct: 686 CPRNDLMTEGSFAVVETLREGGKWAPVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPES 745

Query: 747 ANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 765
           A  GVYR+RH+G+SKS   S +  F+G+SSAF V
Sbjct: 746 AVAGVYRIRHYGASKSLFGSIS-SFSGSSSAFVV 756

BLAST of Carg21861 vs. TAIR 10
Match: AT2G38010.2 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 827.8 bits (2137), Expect = 7.2e-240
Identity = 443/789 (56.15%), Postives = 543/789 (68.82%), Query Frame = 0

Query: 27  WLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFVNLDAG 86
           +L+GVGS+D+TGPAA+VNMMGYAN DQ A+GIHFRLRAR FIVA+   G R+ FVNLDA 
Sbjct: 26  YLIGVGSYDITGPAADVNMMGYANSDQIASGIHFRLRARAFIVAEP-QGNRVVFVNLDAC 85

Query: 87  MASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGGYLQYL 146
           MASQ+VTIK+LERLKA               R+G LYTE+NVAISGIHTHAGPGGYLQY+
Sbjct: 86  MASQIVTIKVLERLKA---------------RYGELYTEKNVAISGIHTHAGPGGYLQYV 145

Query: 147 VYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSAYLMNP 206
            Y +TSLGFV+QSFD +VN IEQSIVQAH SL+PG   +NKG++ +AG+NRSPS+YL NP
Sbjct: 146 TYIVTSLGFVRQSFDVVVNGIEQSIVQAHESLRPGSAFVNKGDLLDAGVNRSPSSYLNNP 205

Query: 207 PEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAARF 266
             ER++Y  +VD EM+LVK V+   G + G+F+WF THGTSMSR N LISGDNKGAAARF
Sbjct: 206 AAERSKYKYDVDKEMTLVKFVDSQLGPT-GSFNWFATHGTSMSRTNSLISGDNKGAAARF 265

Query: 267 FEDWV-------AASNRSTRATSSNKSDI------------------------------- 326
            EDW         +S    R  S+  SD                                
Sbjct: 266 MEDWFENGQKNSVSSRNIPRRVSTIVSDFSRNRESYPFLELPNVAILYIAVAERLMWKVM 325

Query: 327 --------VELMKKAETIKATGGKRCSKTSSQSSKVRKNDGSLFVGAFCQSNVGDVTPNV 386
                     L+  A T K++ G    K+    ++VR      FV AFCQSN GDV+PN 
Sbjct: 326 VLFYFVSESRLLDIAATYKSSRGHSVDKSLDVKTRVRNGSKRKFVSAFCQSNCGDVSPNT 385

Query: 387 LGAFCTDSGKPCDFNRSSCHGSDLLCVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATE 446
           LG FC D+G PCDFN S+C+G + LC GRGPG+PDE  ST+IIGE+QF  AV+LF  ATE
Sbjct: 386 LGTFCIDTGLPCDFNHSTCNGQNELCYGRGPGYPDEFESTRIIGEKQFKMAVELFNKATE 445

Query: 447 RLTGEIDFRHVYLNFTDIEVEL----GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGD 506
           +L G+I ++H YL+F++++V +    GG   VKTCPAA+G GFAAGTTDGPG F F+QGD
Sbjct: 446 KLQGKIGYQHAYLDFSNLDVTVPKAGGGSETVKTCPAAMGFGFAAGTTDGPGAFDFKQGD 505

Query: 507 TQINKLWKKLRDSLRKPSEFQVGCQKPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLII 566
            Q N  W+ +R+ LR P   QV CQKPK +LLDTGEM EPY WAP+ILPIQILR+G+L+I
Sbjct: 506 DQGNVFWRLVRNVLRTPGPEQVQCQKPKPILLDTGEMKEPYDWAPSILPIQILRIGQLVI 565

Query: 567 LSVPGEFTTMAGRRLREAVKETLISNGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQR 626
           LSVPGEFTTMAGRRLR+A+K  LIS+   EF ++ HVVIAGLTN YSQY+AT EEYE QR
Sbjct: 566 LSVPGEFTTMAGRRLRDAIKSFLISSDPKEFSNNMHVVIAGLTNTYSQYIATFEEYEVQR 625

Query: 627 YEAASTLYGPHTLSAYIQEFKKLAIAIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPP 686
           YE ASTLYG HTL+AYIQEFKKLA A+  G  + +    PPDL   Q+  +S   ++S P
Sbjct: 626 YEGASTLYGRHTLTAYIQEFKKLATALVNGLTLPRG-PQPPDLLDKQISLLSPVVVDSTP 685

Query: 687 NGVNFGDIQQDIKLPKVGWFKRGSKQKPTARFWSANPRFDLLTEGTYAVVERL-EKRRWT 746
            GV FGD++ D+  P    F+RG  Q+  A FWS  PR DL+TEG++AVVE L E  +W 
Sbjct: 686 LGVKFGDVKADV--PPKSTFRRG--QQVNATFWSGCPRNDLMTEGSFAVVETLREGGKWA 745

Query: 747 PVYDDDDFSLVFKWALDNNTFTNSLATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYF 765
           PVYDDDDFSL FKW+      + S ATIEW +P  A  GVYR+RH+G+SKS   S +  F
Sbjct: 746 PVYDDDDFSLKFKWSRPAKLSSESQATIEWRVPESAVAGVYRIRHYGASKSLFGSIS-SF 791

BLAST of Carg21861 vs. TAIR 10
Match: AT1G07380.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 807.4 bits (2084), Expect = 1.0e-233
Identity = 423/765 (55.29%), Postives = 544/765 (71.11%), Query Frame = 0

Query: 26  DWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIAFVNLDA 85
           ++L+G+GS+D+TGPAA+VNMMGYANM+Q A+GIHFRLRARTFIV++   G R+ FVNLDA
Sbjct: 36  EYLIGLGSYDITGPAADVNMMGYANMEQVASGIHFRLRARTFIVSEP-QGKRVVFVNLDA 95

Query: 86  GMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGPGGYLQY 145
            MASQ+V +K++ERLKA               R+G+LYTE+NV ISGIHTHAGPGGYLQY
Sbjct: 96  CMASQIVKLKVIERLKA---------------RYGDLYTEQNVGISGIHTHAGPGGYLQY 155

Query: 146 LVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSPSAYLMN 205
           +VY +TSLGFV+QSFDA+V+ IE SI+QAH +L+PG I +N GE+ +AG+NRSPSAYL N
Sbjct: 156 VVYIVTSLGFVRQSFDALVDGIENSIIQAHENLRPGSIFLNNGELLDAGVNRSPSAYLNN 215

Query: 206 PPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDNKGAAAR 265
           P +ER+++  NVD EM+L+K V+   G  +G+F+WF THGTSMSR N LISGDNKGAA+R
Sbjct: 216 PSKERSKHKYNVDKEMTLLKFVDDQWG-PVGSFNWFATHGTSMSRTNSLISGDNKGAASR 275

Query: 266 FFEDWVAASNRSTRATSSNKSDIV----------------ELMKKAETIKATGGKRCSKT 325
           F EDW   +      +    SD +                EL++ A   ++  GK  ++ 
Sbjct: 276 FMEDWYEQNTAERSYSEEFISDEIPRRVSSLIENHQDSHHELLELASYFESQPGKPVTRI 335

Query: 326 SSQSSKV----RKNDGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLL 385
           SS + +V    RK D   FV AFCQ+N GDV+PNVLGAFC D+G PCDFN S+C G + +
Sbjct: 336 SSSARRVRSALRKADKPGFVSAFCQTNCGDVSPNVLGAFCLDTGLPCDFNHSTCGGKNEM 395

Query: 386 CVGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL--- 445
           C GRGPG+PDE  ST+IIGERQF  A++LF  A+E+L G++D+RHVY++F+ + V L   
Sbjct: 396 CYGRGPGYPDEFESTRIIGERQFKMALELFNKASEQLQGKVDYRHVYVDFSQLNVTLPKK 455

Query: 446 -GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQ 505
            G   VVKTCPAA+G  FAAGTTDGPG F F QGD + N  W+ +R+ L+ P + Q+ C 
Sbjct: 456 DGKSEVVKTCPAAMGFAFAAGTTDGPGAFDFTQGDDKGNPFWRLVRNVLKTPDKKQIDCH 515

Query: 506 KPKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLIS 565
            PK +LLDTGEM +PY WAP+IL +Q+LR+G+L ILSVPGEFTTMAGRRLR AVK  L +
Sbjct: 516 YPKPILLDTGEMTKPYDWAPSILSLQVLRIGQLFILSVPGEFTTMAGRRLRYAVKTQLKN 575

Query: 566 NGNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAI 625
           +GN +   + HVVIAGL N YSQYV T EEY+ QRYE ASTLYGPHTLS YIQEFKKL+ 
Sbjct: 576 SGNKDLSGEIHVVIAGLANGYSQYVTTFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLSK 635

Query: 626 AIAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSK 685
           ++     + +P   PPDL   QL F++   +++ P+G +FGD+  D+  PK    KRG+ 
Sbjct: 636 SLVLDMPV-QPGPQPPDLLDKQLSFLTPVMMDTTPSGDSFGDVISDV--PKNLSLKRGNG 695

Query: 686 QKPTARFWSANPRFDLLTEGTYAVVERLEK--RRWTPVYDDDDFSLVFKWALDNNTFTNS 745
           Q  T  F SA PR DLLTEGT+ +VERLE+  + WTPVYDDDD  L FKW+      + S
Sbjct: 696 Q-VTVVFRSACPRNDLLTEGTFTLVERLEQKDKTWTPVYDDDDLCLRFKWSRHKKLSSRS 755

Query: 746 LATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAV 765
            AT+EW IP  A+PGVYR+ HFG++K    S + +FTG+SSAF V
Sbjct: 756 QATVEWRIPESASPGVYRITHFGAAKKLFGSVH-HFTGSSSAFVV 778

BLAST of Carg21861 vs. TAIR 10
Match: AT5G58980.1 (Neutral/alkaline non-lysosomal ceramidase )

HSP 1 Score: 786.9 bits (2031), Expect = 1.4e-227
Identity = 425/765 (55.56%), Postives = 532/765 (69.54%), Query Frame = 0

Query: 20  CCAADGDWLVGVGSFDMTGPAAEVNMMGYANMDQSAAGIHFRLRARTFIVAKSVDGPRIA 79
           C  +D D+L+G+GS+D+TGPAA+VNMMGYANM+Q A+G+HFRLRAR FIVA+     RIA
Sbjct: 22  CIFSDSDYLMGLGSYDITGPAADVNMMGYANMEQVASGVHFRLRARAFIVAEPYK-KRIA 81

Query: 80  FVNLDAGMASQLVTIKLLERLKASLTFLSKLKPRHFIQRFGNLYTEENVAISGIHTHAGP 139
           FVNLDAGMASQLVTIK++ERLK               QR+G LYTEENVAISG HTHAGP
Sbjct: 82  FVNLDAGMASQLVTIKVIERLK---------------QRYGELYTEENVAISGTHTHAGP 141

Query: 140 GGYLQYLVYSITSLGFVQQSFDAIVNAIEQSIVQAHGSLKPGFILINKGEVENAGINRSP 199
           GGYLQY++Y +TSLGFV QSF+A+V+ IEQSI+QAH +L+PG ILINKGE+ +AG+NRSP
Sbjct: 142 GGYLQYILYLVTSLGFVHQSFNALVDGIEQSIIQAHENLRPGSILINKGELLDAGVNRSP 201

Query: 200 SAYLMNPPEERARYPTNVDTEMSLVKVVEGGSGVSLGAFSWFPTHGTSMSRDNKLISGDN 259
           SAYL NP  ER++Y  +VD EM+LVK V+                             D 
Sbjct: 202 SAYLNNPAHERSKYEYDVDKEMTLVKFVD-----------------------------DQ 261

Query: 260 KGAAARFFEDWVAASN-------RSTRATSSNKSDIV--ELMKKAETIKATGGKRCSKTS 319
            G  AR  EDW    N        S R  SS  SD    +LM+ A ++ +TGGK  ++ S
Sbjct: 262 WGPVARIMEDWFERENGCRSVDVESPRRVSSIISDPYDQDLMEMASSLLSTGGKTVTRMS 321

Query: 320 SQSSKVRKN----DGSLFVGAFCQSNVGDVTPNVLGAFCTDSGKPCDFNRSSCHGSDLLC 379
           S + +VR      D   FV AFCQ+N GDV+PNVLGAFC D+G PC+FN+S+C G +  C
Sbjct: 322 SVARRVRSRFRHADKPRFVSAFCQTNCGDVSPNVLGAFCIDTGLPCEFNQSTCGGKNEQC 381

Query: 380 VGRGPGFPDEILSTKIIGERQFLKAVDLFTTATERLTGEIDFRHVYLNFTDIEVEL---- 439
            GRGPG+PDE  ST+IIGERQF KA DLFT A+E + G++D+RH Y++F+ +EV +    
Sbjct: 382 YGRGPGYPDEFESTRIIGERQFKKAADLFTKASEEIQGKVDYRHAYVDFSQLEVTINGQN 441

Query: 440 GGDNVVKTCPAALGPGFAAGTTDGPGVFGFQQGDTQINKLWKKLRDSLRKPSEFQVGCQK 499
           GG  VVKTCPAA+G GFAAGTTDGPG F F+QGD Q N  W+ +R+ L+ P+E QV CQ+
Sbjct: 442 GGSEVVKTCPAAMGFGFAAGTTDGPGAFDFKQGDDQGNPFWRLVRNLLKNPTEEQVRCQR 501

Query: 500 PKTVLLDTGEMFEPYAWAPAILPIQILRLGKLIILSVPGEFTTMAGRRLREAVKETLISN 559
           PK +LLDTGEM +PY WAP+ILP+QILR+G+L+IL VPGEFTTMAGRRLR+AVK  L   
Sbjct: 502 PKPILLDTGEMKQPYDWAPSILPVQILRIGQLVILCVPGEFTTMAGRRLRDAVKTVLKEG 561

Query: 560 GNGEFDDDTHVVIAGLTNAYSQYVATSEEYEQQRYEAASTLYGPHTLSAYIQEFKKLAIA 619
            NG    +  VVIAGLTN+YSQY+AT EEY+ QRYE ASTLYGPHTLS YIQEFKKLA  
Sbjct: 562 SNGR---EFSVVIAGLTNSYSQYIATFEEYQVQRYEGASTLYGPHTLSGYIQEFKKLAND 621

Query: 620 IAKGEKIAKPIVSPPDLSSVQLRFVSDPFIESPPNGVNFGDIQQDIKLPKVGWFKRGSKQ 679
           +   +    P   PPDL   Q+  ++    +  P G  FGD+  D+  P++  F++G+  
Sbjct: 622 LLSAQ-TTDPGPQPPDLLHKQISLLTPVVADMTPIGTAFGDVTSDV--PRLSKFRKGA-D 681

Query: 680 KPTARFWSANPRFDLLTEGTYAVVER-LEKRR-WTPVYDDDDFSLVFKWALDNNTFTNSL 739
               +F SANPR DL+TEGT+A+VER LE R  W PVYDDDDF L FKW+      T S 
Sbjct: 682 IVRVQFRSANPRNDLMTEGTFALVERWLEGRETWVPVYDDDDFCLRFKWSRPFKLSTQST 733

Query: 740 ATIEWDIPIDANPGVYRLRHFGSSKSAINSTNIYFTGASSAFAVF 766
           ATIEW IP  A+PGVYR+ HFGS+K+ I+S + +F+G+SSAF V+
Sbjct: 742 ATIEWRIPETASPGVYRITHFGSAKTPISSIH-HFSGSSSAFVVY 733