Carg21706 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg21706 vs. NCBI nr
Match: KAG7037137.1 (Diacylglycerol kinase 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1545.4 bits (4000), Expect = 0.0e+00
Identity = 759/759 (100.00%), Postives = 759/759 (100.00%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR
Sbjct: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC
Sbjct: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR
Sbjct: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF
Sbjct: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE
Sbjct: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT
Sbjct: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 759

BLAST of Carg21706 vs. NCBI nr
Match: XP_022949086.1 (diacylglycerol kinase 1-like [Cucurbita moschata] >KAG6607481.1 Diacylglycerol kinase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 728/759 (95.92%), Postives = 728/759 (95.92%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR
Sbjct: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC
Sbjct: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR
Sbjct: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLVLINKKSGARRGDSLKQRLNILLNPV                               
Sbjct: 361 PLLVLINKKSGARRGDSLKQRLNILLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE
Sbjct: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT
Sbjct: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 728

BLAST of Carg21706 vs. NCBI nr
Match: XP_022998078.1 (diacylglycerol kinase 1-like [Cucurbita maxima])

HSP 1 Score: 1463.0 bits (3786), Expect = 0.0e+00
Identity = 720/759 (94.86%), Postives = 723/759 (95.26%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDE WEIGLLFPSWNS+NPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR
Sbjct: 1   MDEAWEIGLLFPSWNSKNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKRDPKK NKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPT ASDSFIHRCNIC
Sbjct: 61  SKRDPKKANKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTAASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYNGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR
Sbjct: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLVLINKKSGARRGDSLKQRLNILLNPV                               
Sbjct: 361 PLLVLINKKSGARRGDSLKQRLNILLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSSEQGP SGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSEQGPASGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHG+QLKAPQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGQQLKAPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDK LEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKELEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE
Sbjct: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT
Sbjct: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 728

BLAST of Carg21706 vs. NCBI nr
Match: XP_023524790.1 (diacylglycerol kinase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1461.8 bits (3783), Expect = 0.0e+00
Identity = 718/759 (94.60%), Postives = 724/759 (95.39%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDEVWEIGLLFPSWNS+NPTDRIFVISCLSAAIIG+FTVALTAFLWQRNINLSWMKAIAR
Sbjct: 1   MDEVWEIGLLFPSWNSKNPTDRIFVISCLSAAIIGVFTVALTAFLWQRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKRDPKK NKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC
Sbjct: 61  SKRDPKKANKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYNGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGSRTSEVQHQNGYIDDKNVSNPN+KKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR
Sbjct: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNMKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLVLINKKSGARRGDSLKQRLNILLNPV                               
Sbjct: 361 PLLVLINKKSGARRGDSLKQRLNILLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSSEQGP SGLCLFRKVPHFK+LVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSEQGPASGLCLFRKVPHFKILVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENP KFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPGKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDK LEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKELEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQGKSI+IQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE
Sbjct: 661 GKLQVGLSRARRLAQGKSIKIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT
Sbjct: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 728

BLAST of Carg21706 vs. NCBI nr
Match: XP_022137252.1 (diacylglycerol kinase 1 isoform X1 [Momordica charantia])

HSP 1 Score: 1335.1 bits (3454), Expect = 0.0e+00
Identity = 652/759 (85.90%), Postives = 686/759 (90.38%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDE  EIGLLFPSWNS+NPTDR+F+ISC  AAIIGI T+A TAF W+RNINL WMKAIAR
Sbjct: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKR+PKKTNKVPVAAH WILESVSRGKNL+CC CL+F+SPSQTLGP VASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHL+CSS AQKDCKCVSMIGFEHVMHQWAVRWTEITDQ DE SFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRS+GKK+KHGRK S+ TGT+GSTC +STESTAD+HH  NGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGS  SEVQHQNG +D KN SN N K+ S+ NQKD SHI+G+ LRYEVIE PPDSR
Sbjct: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLV INKKSGARRGDSLKQRLN+LLNP                                
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPA------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSS QGPESGL LFRKVPHFKVLVCGGDGTVGWVL+CIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQ GKQLK+PQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA+SIMDR+FADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLV NIGSYMGGVDLWHNEDET DNFDPQSMHDKVLEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQG+SI+IQLCA+LPVQIDGEPWFQN CTLVISHHGQAFMLK+A+EE
Sbjct: 661 GKLQVGLSRARRLAQGQSIKIQLCASLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAI+TDVLESAESNNVIN +QKR LLQEMAIRLT
Sbjct: 721 PLGHAAAIVTDVLESAESNNVINATQKRALLQEMAIRLT 728

BLAST of Carg21706 vs. ExPASy Swiss-Prot
Match: Q39017 (Diacylglycerol kinase 1 OS=Arabidopsis thaliana OX=3702 GN=DGK1 PE=1 SV=2)

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 529/767 (68.97%), Postives = 611/767 (79.66%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRI----FVISCLSAAIIGIFTVALTAFLWQRNINLSWMK 60
           MD+  E+G+ FPSW S+NP D +     + SC  AA++GI T+A TAF W+RNINLSW K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHR 120
           AIARSK++PK  +KVPVA H W L+ ++R KNL+CC CLK +SPSQ +   VAS+SF HR
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CNICGAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCS 180
           C ICGAAAH NCSSSA KDCKCVSM+GFEHV+HQWAVRWTE  DQ+D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGG 240
            SFLGGSPIWCCLWCQRLVHVDCHS+M NETGDICDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANG 300
           FLSSITHGANE+AS+  ASIR Q KK K   ++S  TG SGS C  STESTAD+  T NG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YHGTERN----CNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEV 360
            H    N     NG  ++     NG ++ K    P++K+  SF QK+  H +   L+YE+
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKK----PSVKRTGSFGQKEY-HALRSKLKYEL 360

Query: 361 IEFPPDSRPLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLM 420
            + P D+RPLLV INKKSGA+RGDSL+QRL++ LNPV                       
Sbjct: 361 ADLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPV----------------------- 420

Query: 421 VMDCMLIFQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPP 480
                   QVFELSS QGPE GL LFRKVPHF+VLVCGGDGT GWVLD I+KQNF+SPP 
Sbjct: 421 --------QVFELSSVQGPEVGLFLFRKVPHFRVLVCGGDGTAGWVLDAIEKQNFISPPA 480

Query: 481 VAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQ 540
           VAILPAGTGNDL+RVLNWGGGLGSVERQGGL TVL ++E+AAVTVLDRWKV+I++Q GKQ
Sbjct: 481 VAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQ 540

Query: 541 LKAPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADI 600
           L+ P++MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGARSIMDR+F D 
Sbjct: 541 LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDF 600

Query: 601 PWQVRVEVDGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVV 660
           PWQVRVEVDGV++EVPEDAEG+LV NIGSYMGGVDLW NEDET +NFDPQSMHDK++EVV
Sbjct: 601 PWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVV 660

Query: 661 SISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAF 720
           SISGTWHLGKLQVGLSRARRLAQG +++IQLCA LPVQIDGEPW Q PCTL ISHHGQAF
Sbjct: 661 SISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAF 720

Query: 721 MLKKAIEEPLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           MLK+A EEPLGHAAAI+TDVLE+AE+N VIN SQKR LLQEMA+RLT
Sbjct: 721 MLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT 728

BLAST of Carg21706 vs. ExPASy Swiss-Prot
Match: Q9FFN7 (Diacylglycerol kinase 2 OS=Arabidopsis thaliana OX=3702 GN=DGK2 PE=1 SV=1)

HSP 1 Score: 691.0 bits (1782), Expect = 1.5e-197
Identity = 363/761 (47.70%), Postives = 480/761 (63.07%), Query Frame = 0

Query: 6   EIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIARSKRDP 65
           E+G     W   +  D  F+   L    +G+  V  T   WQ+  +L+W+KA AR K+  
Sbjct: 3   EVGFSLIQWLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKV 62

Query: 66  KKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNICGAAAH 125
            K  +VP++ H W  +     +  +CC CL  + P Q +    +    +HRC +CG AAH
Sbjct: 63  WKRLRVPLSHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAH 122

Query: 126 LNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFLGGSPI 185
             CSSSA KDCKCV+  G +HV H W+ RW  + D +D  +FC YC+EPC   F+  SP+
Sbjct: 123 FYCSSSAAKDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPM 182

Query: 186 WCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSSITHGA 245
           W CLWCQRL+HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    
Sbjct: 183 WHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---K 242

Query: 246 NEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGTERNCN 305
           NE+A S+R  +R +  + K+G   S+    +G    L  +S +D   T           N
Sbjct: 243 NELA-SIRGHVRRKRHRGKNGNGQSL----NGK---LLEDSVSDPVKTV---------VN 302

Query: 306 GSRTSEVQHQNGYIDDKNVSN-PNIKKYSS---FNQKDVSHIVGMNLRYEVIEFPPDSRP 365
           G    +++        K VS+ PN K   +    ++++ S  +    ++ +++ PPD+RP
Sbjct: 303 GLVVKKLRRDRSIDCLKQVSDMPNAKGLQNGIGGHKRNKSAALNFMKKFSLVDLPPDARP 362

Query: 366 LLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIFQ 425
           LLV IN KSG + G  L +RLN+LLNPV                               Q
Sbjct: 363 LLVFINAKSGGQLGPFLHRRLNMLLNPV-------------------------------Q 422

Query: 426 VFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGTG 485
           VFEL S QGP++GL L  KV +F+VLVCGGDGTV WVLD I+K+NF SPPPVAILP GTG
Sbjct: 423 VFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFESPPPVAILPLGTG 482

Query: 486 NDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQ---F 545
           NDL+RVL WG G+  V+ QG L T L  +++AAVT+LDRW V IV++  ++  A +   F
Sbjct: 483 NDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKF 542

Query: 546 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRV 605
           M NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGAR IMDR+ AD+PWQV +
Sbjct: 543 MMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWL 602

Query: 606 EVDGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTW 665
           EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+ E  DNF  Q MHDK LEVV + G W
Sbjct: 603 EVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAW 662

Query: 666 HLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAI 725
           HLGKLQVGLS+ARRLAQGK IRI + +  PVQIDGEP+ Q P  L I+HHGQ FML++A 
Sbjct: 663 HLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQPGCLEITHHGQVFMLRRAS 712

Query: 726 EEPLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           +EP GHAAAIM +VL  AE   VIN SQK+VLLQ+MA+ L+
Sbjct: 723 DEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQMALHLS 712

BLAST of Carg21706 vs. ExPASy Swiss-Prot
Match: P49620 (Diacylglycerol kinase gamma OS=Rattus norvegicus OX=10116 GN=Dgkg PE=1 SV=1)

HSP 1 Score: 261.2 bits (666), Expect = 3.8e-68
Identity = 152/391 (38.87%), Postives = 209/391 (53.45%), Query Frame = 0

Query: 348 LRYEVIEFPPDSRPLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMM 407
           ++Y++I   P + PLLVL+N KSG R+G+ + Q+ + LLNP                   
Sbjct: 419 MQYKIIP-SPGTHPLLVLVNPKSGGRQGERILQKFHYLLNPK------------------ 478

Query: 408 FMNLMVMDCMLIFQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNF 467
                        QVF L  + GP  GL  F+  P F+VL CGGDGTVGW+LDCIDK NF
Sbjct: 479 -------------QVFNL-DKGGPTPGLNFFQDTPDFRVLACGGDGTVGWILDCIDKANF 538

Query: 468 VSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVD 527
              PPVA+LP GTGNDLAR L WGGG       G L  +L  +E + + +LDRW + ++ 
Sbjct: 539 TKHPPVAVLPLGTGNDLARCLRWGGGY----EGGSLTKILKEIEQSPLVMLDRWYLEVMP 598

Query: 528 ----QHGKQLKAPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARS 587
               ++G Q+     MNNY  IG DA +A   H +RE++PEKF ++  NK+ Y   G   
Sbjct: 599 REEVENGDQVPY-NIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSE 658

Query: 588 IMDRSFADIPWQVRVEVDGVEVEVPE-DAEGVLVTNIGSYMGGVDLWHNEDETSDN---- 647
               +   +   + +E DGVEV++     EG+ + NI S  GG +LW    ET  N    
Sbjct: 659 TFAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLW---GETKKNRAVI 718

Query: 648 -------FDP-------QSMHDKVLEVVSISGTWHLGKLQVGL-SRARRLAQGKSIRIQL 707
                   DP       Q + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+ 
Sbjct: 719 RESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRT 768

Query: 708 CAALPVQIDGEPWFQNPCTLVISHHGQAFML 715
              LP+Q+DGEPW Q PC + I+H  QA M+
Sbjct: 779 KKLLPMQVDGEPWMQPPCMIKITHKNQAPMM 768

BLAST of Carg21706 vs. ExPASy Swiss-Prot
Match: Q91WG7 (Diacylglycerol kinase gamma OS=Mus musculus OX=10090 GN=Dgkg PE=1 SV=1)

HSP 1 Score: 258.1 bits (658), Expect = 3.2e-67
Identity = 151/390 (38.72%), Postives = 207/390 (53.08%), Query Frame = 0

Query: 349 RYEVIEFPPDSRPLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMF 408
           +Y++I   P + PLLVL+N KSG R+G+ + ++ + LLNP                    
Sbjct: 420 QYKIIP-SPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPE------------------- 479

Query: 409 MNLMVMDCMLIFQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFV 468
                       QVF L +  GP  GL  F   P F+VL CGGDGTVGW+LDCIDK NF 
Sbjct: 480 ------------QVFNLDN-GGPTPGLNFFHDTPDFRVLACGGDGTVGWILDCIDKANFT 539

Query: 469 SPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVD- 528
             PPVA+LP GTGNDLAR L WGGG       G L  +L  +E + + +LDRW + ++  
Sbjct: 540 KHPPVAVLPLGTGNDLARCLRWGGGY----EGGSLTKILKEIEQSPLVMLDRWYLEVMPR 599

Query: 529 ---QHGKQLKAPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSI 588
              ++G Q+     MNNY  IG DA +A   H +RE++PEKF ++  NK+ Y   G    
Sbjct: 600 EEVENGDQVPY-NIMNNYFSIGVDASIAHRFHMMREKHPEKFNSRMKNKLWYFEFGTSET 659

Query: 589 MDRSFADIPWQVRVEVDGVEVEVPE-DAEGVLVTNIGSYMGGVDLWHNEDETSDN----- 648
              +   +   + +E DGVEV++     EG+ + NI S  GG +LW    ET  N     
Sbjct: 660 FAATCKKLHDHIELECDGVEVDLSNIFLEGIAILNIPSMYGGTNLW---GETKKNRAVIR 719

Query: 649 ------FDP-------QSMHDKVLEVVSISGTWHLGKLQVGL-SRARRLAQGKSIRIQLC 708
                  DP       Q + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+  
Sbjct: 720 ESRKSVTDPKELKCCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCSSVTIRTN 768

Query: 709 AALPVQIDGEPWFQNPCTLVISHHGQAFML 715
             LP+Q+DGEPW Q  CT+ I+H  QA M+
Sbjct: 780 KLLPMQVDGEPWMQPQCTIKITHKNQAPMM 768

BLAST of Carg21706 vs. ExPASy Swiss-Prot
Match: P49619 (Diacylglycerol kinase gamma OS=Homo sapiens OX=9606 GN=DGKG PE=1 SV=3)

HSP 1 Score: 257.7 bits (657), Expect = 4.2e-67
Identity = 148/388 (38.14%), Postives = 205/388 (52.84%), Query Frame = 0

Query: 348 LRYEVIEFPPDSRPLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMM 407
           ++Y++I   P + PLLVL+N KSG R+G+ + ++ + LLNP                   
Sbjct: 422 MQYKIIP-TPGTHPLLVLVNPKSGGRQGERILRKFHYLLNPK------------------ 481

Query: 408 FMNLMVMDCMLIFQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNF 467
                        QVF L +  GP  GL  FR  P F+VL CGGDGTVGW+LDCIDK NF
Sbjct: 482 -------------QVFNLDN-GGPTPGLNFFRDTPDFRVLACGGDGTVGWILDCIDKANF 541

Query: 468 VSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVD 527
              PPVA+LP GTGNDLAR L WGGG       G L  +L  +E + + +LDRW + ++ 
Sbjct: 542 AKHPPVAVLPLGTGNDLARCLRWGGGY----EGGSLTKILKDIEQSPLVMLDRWHLEVIP 601

Query: 528 ----QHGKQLKAPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARS 587
               ++G Q+     MNNY  IG DA +A   H +RE++PEKF ++  NK+ Y   G   
Sbjct: 602 REEVENGDQVPY-SIMNNYFSIGVDASIAHRFHVMREKHPEKFNSRMKNKLWYFEFGTSE 661

Query: 588 IMDRSFADIPWQVRVEVDGVEVEVPE-DAEGVLVTNIGSYMGGVDLWHNE---------- 647
               +   +   + +E DGV V++     EG+ + NI S  GG +LW             
Sbjct: 662 TFAATCKKLHDHIELECDGVGVDLSNIFLEGIAILNIPSMYGGTNLWGENKKNRAVIRES 721

Query: 648 -----DETSDNFDPQSMHDKVLEVVSISGTWHLGKLQVGL-SRARRLAQGKSIRIQLCAA 707
                D     F  Q + D++LEVV + G   +G++  GL S  RRLAQ  S+ I+    
Sbjct: 722 RKGVTDPKELKFCVQDLSDQLLEVVGLEGAMEMGQIYTGLKSAGRRLAQCASVTIRTNKL 771

Query: 708 LPVQIDGEPWFQNPCTLVISHHGQAFML 715
           LP+Q+DGEPW Q  CT+ I+H  QA M+
Sbjct: 782 LPMQVDGEPWMQPCCTIKITHKNQAPMM 771

BLAST of Carg21706 vs. ExPASy TrEMBL
Match: A0A6J1GB06 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111452543 PE=3 SV=1)

HSP 1 Score: 1478.0 bits (3825), Expect = 0.0e+00
Identity = 728/759 (95.92%), Postives = 728/759 (95.92%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR
Sbjct: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC
Sbjct: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR
Sbjct: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLVLINKKSGARRGDSLKQRLNILLNPV                               
Sbjct: 361 PLLVLINKKSGARRGDSLKQRLNILLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE
Sbjct: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT
Sbjct: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 728

BLAST of Carg21706 vs. ExPASy TrEMBL
Match: A0A6J1KFT1 (Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111492833 PE=3 SV=1)

HSP 1 Score: 1463.0 bits (3786), Expect = 0.0e+00
Identity = 720/759 (94.86%), Postives = 723/759 (95.26%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDE WEIGLLFPSWNS+NPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR
Sbjct: 1   MDEAWEIGLLFPSWNSKNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKRDPKK NKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPT ASDSFIHRCNIC
Sbjct: 61  SKRDPKKANKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTAASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYNGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR
Sbjct: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLVLINKKSGARRGDSLKQRLNILLNPV                               
Sbjct: 361 PLLVLINKKSGARRGDSLKQRLNILLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSSEQGP SGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSEQGPASGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHG+QLKAPQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGQQLKAPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDK LEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKELEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE
Sbjct: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT
Sbjct: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 728

BLAST of Carg21706 vs. ExPASy TrEMBL
Match: A0A6J1C7R2 (Diacylglycerol kinase OS=Momordica charantia OX=3673 GN=LOC111008758 PE=3 SV=1)

HSP 1 Score: 1335.1 bits (3454), Expect = 0.0e+00
Identity = 652/759 (85.90%), Postives = 686/759 (90.38%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDE  EIGLLFPSWNS+NPTDR+F+ISC  AAIIGI T+A TAF W+RNINL WMKAIAR
Sbjct: 1   MDEDSEIGLLFPSWNSKNPTDRLFIISCFIAAIIGILTIAFTAFQWRRNINLGWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKR+PKKTNKVPVAAH WILESVSRGKNL+CC CL+F+SPSQTLGP VASDSFIH C+IC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLNCCVCLRFMSPSQTLGPMVASDSFIHCCSIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           GAAAHL+CSS AQKDCKCVSMIGFEHVMHQWAVRWTEITDQ DE SFCSYCEEPCSGSFL
Sbjct: 121 GAAAHLSCSSGAQKDCKCVSMIGFEHVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRS+GKK+KHGRK S+ TGT+GSTC +STESTAD+HH  NGYHGT
Sbjct: 241 ITHGANEIASSVRASIRSKGKKHKHGRKPSIDTGTTGSTCDMSTESTADAHHRVNGYHGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERNCNGS  SEVQHQNG +D KN SN N K+ S+ NQKD SHI+G+ LRYEVIE PPDSR
Sbjct: 301 ERNCNGSAASEVQHQNGDMDGKNSSNQNFKRSSAVNQKDESHILGLKLRYEVIELPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLV INKKSGARRGDSLKQRLN+LLNP                                
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPA------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSS QGPESGL LFRKVPHFKVLVCGGDGTVGWVL+CIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQ GKQLK+PQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA+SIMDR+FADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTFADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLV NIGSYMGGVDLWHNEDET DNFDPQSMHDKVLEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDNFDPQSMHDKVLEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAIEE 720
           GKLQVGLSRARRLAQG+SI+IQLCA+LPVQIDGEPWFQN CTLVISHHGQAFMLK+A+EE
Sbjct: 661 GKLQVGLSRARRLAQGQSIKIQLCASLPVQIDGEPWFQNQCTLVISHHGQAFMLKRAVEE 720

Query: 721 PLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           PLGHAAAI+TDVLESAESNNVIN +QKR LLQEMAIRLT
Sbjct: 721 PLGHAAAIVTDVLESAESNNVINATQKRALLQEMAIRLT 728

BLAST of Carg21706 vs. ExPASy TrEMBL
Match: A0A6J1E7Q5 (Diacylglycerol kinase OS=Cucurbita moschata OX=3662 GN=LOC111431571 PE=3 SV=1)

HSP 1 Score: 1332.4 bits (3447), Expect = 0.0e+00
Identity = 657/760 (86.45%), Postives = 690/760 (90.79%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDE WEIGLLFPSWNS+NPTDR+FVISCLSAAIIGI T+A TAF W+RNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKR+PKKTNKVPVAAH WILESVSRGKNLSCC CLKFVSPSQTLGP VASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           G AAHL+CSS+AQKDCKCVSMIGFE+VMHQWAVRWTEITDQ DE SFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLYVKES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYVKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIASSVRASIRSQ KKNKH RK S++   SGS   +STESTADSHHT NGY+GT
Sbjct: 241 ITHGANEIASSVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERN NGSRTSE+QHQNG IDDKN+SN + KK SSFNQKDV+H VGMNLRYEVIE PPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDIDDKNISNSSFKKNSSFNQKDVTH-VGMNLRYEVIELPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLV INKKSGARRGDSLKQRLN+LLNPV                               
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSS QGPESGL LFRKVPHFKVLVCGGDGTVGWVL+CIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVL HVENAAVTVLDRWKVAIVDQ GKQLK+PQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA+SIMDR++ADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTYADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLV NIGSYMGGVDLWHNEDET D+FD QSMHDK+LEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDSFDSQSMHDKILEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQN-PCTLVISHHGQAFMLKKAIE 720
           GKLQVGLSRARRLAQG+SIRIQLCAALPVQIDGEPWFQ+ PCTLVISHHG+AFMLK+A+E
Sbjct: 661 GKLQVGLSRARRLAQGRSIRIQLCAALPVQIDGEPWFQDIPCTLVISHHGEAFMLKRAVE 720

Query: 721 EPLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           EPLGHAAAI+TDVLESAESNNVIN SQKRVLLQE+A RLT
Sbjct: 721 EPLGHAAAIITDVLESAESNNVINASQKRVLLQEIAKRLT 728

BLAST of Carg21706 vs. ExPASy TrEMBL
Match: A0A6J1KN74 (Diacylglycerol kinase OS=Cucurbita maxima OX=3661 GN=LOC111495021 PE=3 SV=1)

HSP 1 Score: 1325.1 bits (3428), Expect = 0.0e+00
Identity = 654/760 (86.05%), Postives = 689/760 (90.66%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIAR 60
           MDE WEIGLLFPSWNS+NPTDR+FVISCLSAAIIGI T+A TAF W+RNINLSWMKAIAR
Sbjct: 1   MDEEWEIGLLFPSWNSKNPTDRLFVISCLSAAIIGILTIAFTAFQWRRNINLSWMKAIAR 60

Query: 61  SKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNIC 120
           SKR+PKKTNKVPVAAH WILESVSRGKNLSCC CLKFVSPSQTLGP VASDSFIHRCNIC
Sbjct: 61  SKRNPKKTNKVPVAAHDWILESVSRGKNLSCCVCLKFVSPSQTLGPMVASDSFIHRCNIC 120

Query: 121 GAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFL 180
           G AAHL+CSS+AQKDCKCVSMIGFE+VMHQWAVRWTEITDQ DE SFCSYCEEPCSGSFL
Sbjct: 121 GVAAHLSCSSNAQKDCKCVSMIGFENVMHQWAVRWTEITDQPDETSFCSYCEEPCSGSFL 180

Query: 181 GGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSS 240
           GGSPIWCCLWCQR+VHVDCHSSMCNETGD+CDLGSFRRLILSPLY+KES RTSSGGFLSS
Sbjct: 181 GGSPIWCCLWCQRVVHVDCHSSMCNETGDVCDLGSFRRLILSPLYIKESNRTSSGGFLSS 240

Query: 241 ITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGT 300
           ITHGANEIAS+VRASIRSQ KKNKH RK S++   SGS   +STESTADSHHT NGY+GT
Sbjct: 241 ITHGANEIASTVRASIRSQSKKNKHSRKPSIHASKSGSIRDMSTESTADSHHTVNGYNGT 300

Query: 301 ERNCNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEVIEFPPDSR 360
           ERN NGSRTSE+QHQNG I DKN+SNP+ KK SSFNQKD +H VGMNLRYEVIE PPDSR
Sbjct: 301 ERNINGSRTSELQHQNGDI-DKNISNPSFKKSSSFNQKDETH-VGMNLRYEVIELPPDSR 360

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PLLV INKKSGARRGDSLKQRLN+LLNPV                               
Sbjct: 361 PLLVFINKKSGARRGDSLKQRLNMLLNPV------------------------------- 420

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGT 480
           QVFELSS QGPESGL LFRKVPHFKVLVCGGDGTVGWVL+CIDKQNFVSPPPVAILPAGT
Sbjct: 421 QVFELSSAQGPESGLYLFRKVPHFKVLVCGGDGTVGWVLNCIDKQNFVSPPPVAILPAGT 480

Query: 481 GNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQFMN 540
           GNDLARVLNWGGGLGSVERQGGLCTVL HVENAAVTVLDRWKVAIVDQ GKQLK+PQFMN
Sbjct: 481 GNDLARVLNWGGGLGSVERQGGLCTVLRHVENAAVTVLDRWKVAIVDQQGKQLKSPQFMN 540

Query: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRVEV 600
           NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGA+SIMDR++ADIPWQVRVEV
Sbjct: 541 NYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGAKSIMDRTYADIPWQVRVEV 600

Query: 601 DGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTWHL 660
           DGVEVEVPEDAEGVLV NIGSYMGGVDLWHNEDET D+FD QSMHDK+LEVVSISGTWHL
Sbjct: 601 DGVEVEVPEDAEGVLVANIGSYMGGVDLWHNEDETFDSFDSQSMHDKILEVVSISGTWHL 660

Query: 661 GKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQN-PCTLVISHHGQAFMLKKAIE 720
           GKLQVGLSRARRLAQG+SIRIQLCAALPVQIDGEPWFQ+ PCTLVISHHG+AFMLK+A+E
Sbjct: 661 GKLQVGLSRARRLAQGRSIRIQLCAALPVQIDGEPWFQDIPCTLVISHHGEAFMLKRAVE 720

Query: 721 EPLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           EPLGHAAAI+TDVLESAESNNVIN SQKRVLLQE+A RLT
Sbjct: 721 EPLGHAAAIITDVLESAESNNVINASQKRVLLQEIAKRLT 727

BLAST of Carg21706 vs. TAIR 10
Match: AT5G07920.1 (diacylglycerol kinase1 )

HSP 1 Score: 1076.6 bits (2783), Expect = 0.0e+00
Identity = 529/767 (68.97%), Postives = 611/767 (79.66%), Query Frame = 0

Query: 1   MDEVWEIGLLFPSWNSRNPTDRI----FVISCLSAAIIGIFTVALTAFLWQRNINLSWMK 60
           MD+  E+G+ FPSW S+NP D +     + SC  AA++GI T+A TAF W+RNINLSW K
Sbjct: 1   MDDDGELGMFFPSWTSKNPIDTVESRGLMFSCFVAALVGILTIAYTAFQWRRNINLSWTK 60

Query: 61  AIARSKRDPKKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHR 120
           AIARSK++PK  +KVPVA H W L+ ++R KNL+CC CLK +SPSQ +   VAS+SF HR
Sbjct: 61  AIARSKKNPKARHKVPVAPHSWELDPIARAKNLNCCVCLKSMSPSQAI---VASESFFHR 120

Query: 121 CNICGAAAHLNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCS 180
           C ICGAAAH NCSSSA KDCKCVSM+GFEHV+HQWAVRWTE  DQ+D++SFCSYC+E CS
Sbjct: 121 CTICGAAAHFNCSSSAPKDCKCVSMVGFEHVVHQWAVRWTEGADQTDDSSFCSYCDESCS 180

Query: 181 GSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGG 240
            SFLGGSPIWCCLWCQRLVHVDCHS+M NETGDICDLG  RRLIL PLYVKE  R  SGG
Sbjct: 181 SSFLGGSPIWCCLWCQRLVHVDCHSNMSNETGDICDLGPLRRLILCPLYVKELTRNPSGG 240

Query: 241 FLSSITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANG 300
           FLSSITHGANE+AS+  ASIR Q KK K   ++S  TG SGS C  STESTAD+  T NG
Sbjct: 241 FLSSITHGANELASTALASIRIQSKKYKQTNETSADTGNSGSNCDESTESTADTGPTVNG 300

Query: 301 YHGTERN----CNGSRTSEVQHQNGYIDDKNVSNPNIKKYSSFNQKDVSHIVGMNLRYEV 360
            H    N     NG  ++     NG ++ K    P++K+  SF QK+  H +   L+YE+
Sbjct: 301 AHAVLENSISVMNGDSSNGDSDSNGKLEKK----PSVKRTGSFGQKEY-HALRSKLKYEL 360

Query: 361 IEFPPDSRPLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLM 420
            + P D+RPLLV INKKSGA+RGDSL+QRL++ LNPV                       
Sbjct: 361 ADLPSDARPLLVFINKKSGAQRGDSLRQRLHLHLNPV----------------------- 420

Query: 421 VMDCMLIFQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPP 480
                   QVFELSS QGPE GL LFRKVPHF+VLVCGGDGT GWVLD I+KQNF+SPP 
Sbjct: 421 --------QVFELSSVQGPEVGLFLFRKVPHFRVLVCGGDGTAGWVLDAIEKQNFISPPA 480

Query: 481 VAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQ 540
           VAILPAGTGNDL+RVLNWGGGLGSVERQGGL TVL ++E+AAVTVLDRWKV+I++Q GKQ
Sbjct: 481 VAILPAGTGNDLSRVLNWGGGLGSVERQGGLSTVLQNIEHAAVTVLDRWKVSILNQQGKQ 540

Query: 541 LKAPQFMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADI 600
           L+ P++MNNY+G+GCDAKVAL+IHNLREENPE+FY+QFMNKVLYAREGARSIMDR+F D 
Sbjct: 541 LQPPKYMNNYIGVGCDAKVALEIHNLREENPERFYSQFMNKVLYAREGARSIMDRTFEDF 600

Query: 601 PWQVRVEVDGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVV 660
           PWQVRVEVDGV++EVPEDAEG+LV NIGSYMGGVDLW NEDET +NFDPQSMHDK++EVV
Sbjct: 601 PWQVRVEVDGVDIEVPEDAEGILVANIGSYMGGVDLWQNEDETYENFDPQSMHDKIVEVV 660

Query: 661 SISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAF 720
           SISGTWHLGKLQVGLSRARRLAQG +++IQLCA LPVQIDGEPW Q PCTL ISHHGQAF
Sbjct: 661 SISGTWHLGKLQVGLSRARRLAQGSAVKIQLCAPLPVQIDGEPWNQQPCTLTISHHGQAF 720

Query: 721 MLKKAIEEPLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           MLK+A EEPLGHAAAI+TDVLE+AE+N VIN SQKR LLQEMA+RLT
Sbjct: 721 MLKRAAEEPLGHAAAIITDVLENAETNQVINASQKRTLLQEMALRLT 728

BLAST of Carg21706 vs. TAIR 10
Match: AT5G63770.1 (diacylglycerol kinase 2 )

HSP 1 Score: 691.0 bits (1782), Expect = 1.0e-198
Identity = 363/761 (47.70%), Postives = 480/761 (63.07%), Query Frame = 0

Query: 6   EIGLLFPSWNSRNPTDRIFVISCLSAAIIGIFTVALTAFLWQRNINLSWMKAIARSKRDP 65
           E+G     W   +  D  F+   L    +G+  V  T   WQ+  +L+W+KA AR K+  
Sbjct: 3   EVGFSLIQWLISSGADSPFIFGWLVTGSVGLLAVIYTFLKWQKKTSLNWVKAAAREKKKV 62

Query: 66  KKTNKVPVAAHYWILESVSRGKNLSCCACLKFVSPSQTLGPTVASDSFIHRCNICGAAAH 125
            K  +VP++ H W  +     +  +CC CL  + P Q +    +    +HRC +CG AAH
Sbjct: 63  WKRLRVPLSHHQWTDDYGYGQQPSTCCVCLYSLVPGQNVSNKASLSIPVHRCAVCGVAAH 122

Query: 126 LNCSSSAQKDCKCVSMIGFEHVMHQWAVRWTEITDQSDEASFCSYCEEPCSGSFLGGSPI 185
             CSSSA KDCKCV+  G +HV H W+ RW  + D +D  +FC YC+EPC   F+  SP+
Sbjct: 123 FYCSSSAAKDCKCVAQAGSDHVRHHWSERWVNMDDNADMTAFCFYCDEPCGIPFIEASPM 182

Query: 186 WCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRLILSPLYVKESIRTSSGGFLSSITHGA 245
           W CLWCQRL+HV CH  M  E+GD CDLGS RR+ILSP++VK +      G L++I    
Sbjct: 183 WHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRVILSPVHVKLNEANGVDGVLTTI---K 242

Query: 246 NEIASSVRASIRSQGKKNKHGRKSSVYTGTSGSTCGLSTESTADSHHTANGYHGTERNCN 305
           NE+A S+R  +R +  + K+G   S+    +G    L  +S +D   T           N
Sbjct: 243 NELA-SIRGHVRRKRHRGKNGNGQSL----NGK---LLEDSVSDPVKTV---------VN 302

Query: 306 GSRTSEVQHQNGYIDDKNVSN-PNIKKYSS---FNQKDVSHIVGMNLRYEVIEFPPDSRP 365
           G    +++        K VS+ PN K   +    ++++ S  +    ++ +++ PPD+RP
Sbjct: 303 GLVVKKLRRDRSIDCLKQVSDMPNAKGLQNGIGGHKRNKSAALNFMKKFSLVDLPPDARP 362

Query: 366 LLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIFQ 425
           LLV IN KSG + G  L +RLN+LLNPV                               Q
Sbjct: 363 LLVFINAKSGGQLGPFLHRRLNMLLNPV-------------------------------Q 422

Query: 426 VFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDKQNFVSPPPVAILPAGTG 485
           VFEL S QGP++GL L  KV +F+VLVCGGDGTV WVLD I+K+NF SPPPVAILP GTG
Sbjct: 423 VFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTVAWVLDAIEKRNFESPPPVAILPLGTG 482

Query: 486 NDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAPQ---F 545
           NDL+RVL WG G+  V+ QG L T L  +++AAVT+LDRW V IV++  ++  A +   F
Sbjct: 483 NDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAVTMLDRWSVKIVEESTEKFPAREGHKF 542

Query: 546 MNNYLGIGCDAKVALDIHNLREENPEKFYNQFMNKVLYAREGARSIMDRSFADIPWQVRV 605
           M NYLGIGCDAKVA + H +R+E PEKF +QF+NK+ YA+EGAR IMDR+ AD+PWQV +
Sbjct: 543 MMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVNKLRYAKEGARDIMDRACADLPWQVWL 602

Query: 606 EVDGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNEDETSDNFDPQSMHDKVLEVVSISGTW 665
           EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+ E  DNF  Q MHDK LEVV + G W
Sbjct: 603 EVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQNDYEHDDNFSIQCMHDKTLEVVCVRGAW 662

Query: 666 HLGKLQVGLSRARRLAQGKSIRIQLCAALPVQIDGEPWFQNPCTLVISHHGQAFMLKKAI 725
           HLGKLQVGLS+ARRLAQGK IRI + +  PVQIDGEP+ Q P  L I+HHGQ FML++A 
Sbjct: 663 HLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQIDGEPFIQQPGCLEITHHGQVFMLRRAS 712

Query: 726 EEPLGHAAAIMTDVLESAESNNVINTSQKRVLLQEMAIRLT 760
           +EP GHAAAIM +VL  AE   VIN SQK+VLLQ+MA+ L+
Sbjct: 723 DEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQQMALHLS 712

BLAST of Carg21706 vs. TAIR 10
Match: AT5G63770.2 (diacylglycerol kinase 2 )

HSP 1 Score: 574.7 bits (1480), Expect = 1.1e-163
Identity = 308/607 (50.74%), Postives = 400/607 (65.90%), Query Frame = 0

Query: 160 DQSDEASFCSYCEEPCSGSFLGGSPIWCCLWCQRLVHVDCHSSMCNETGDICDLGSFRRL 219
           D +D  +FC YC+EPC   F+  SP+W CLWCQRL+HV CH  M  E+GD CDLGS RR+
Sbjct: 3   DNADMTAFCFYCDEPCGIPFIEASPMWHCLWCQRLIHVKCHMIMSKESGDACDLGSLRRV 62

Query: 220 ILSPLYVKESIRTSSGGFLSSITHGANEIASSVRASIRSQGKKNKHGRKSSVYTGTSGST 279
           ILSP++VK +      G L++I    NE+A S+R  +R +  + K+G   S+    +G  
Sbjct: 63  ILSPVHVKLNEANGVDGVLTTI---KNELA-SIRGHVRRKRHRGKNGNGQSL----NGK- 122

Query: 280 CGLSTESTADSHHTANGYHGTERNCNGSRTSEVQHQNGYIDDKNVSN-PNIKKYSS---F 339
             L  +S +D   T           NG    +++        K VS+ PN K   +    
Sbjct: 123 --LLEDSVSDPVKTV---------VNGLVVKKLRRDRSIDCLKQVSDMPNAKGLQNGIGG 182

Query: 340 NQKDVSHIVGMNLRYEVIEFPPDSRPLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNL 399
           ++++ S  +    ++ +++ PPD+RPLLV IN KSG + G  L +RLN+LLNPV      
Sbjct: 183 HKRNKSAALNFMKKFSLVDLPPDARPLLVFINAKSGGQLGPFLHRRLNMLLNPV------ 242

Query: 400 LRILFSLQVLMMFMNLMVMDCMLIFQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTV 459
                                    QVFEL S QGP++GL L  KV +F+VLVCGGDGTV
Sbjct: 243 -------------------------QVFELGSCQGPDAGLDLCSKVKYFRVLVCGGDGTV 302

Query: 460 GWVLDCIDKQNFVSPPPVAILPAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAV 519
            WVLD I+K+NF SPPPVAILP GTGNDL+RVL WG G+  V+ QG L T L  +++AAV
Sbjct: 303 AWVLDAIEKRNFESPPPVAILPLGTGNDLSRVLQWGRGISVVDGQGSLRTFLQDIDHAAV 362

Query: 520 TVLDRWKVAIVDQHGKQLKAPQ---FMNNYLGIGCDAKVALDIHNLREENPEKFYNQFMN 579
           T+LDRW V IV++  ++  A +   FM NYLGIGCDAKVA + H +R+E PEKF +QF+N
Sbjct: 363 TMLDRWSVKIVEESTEKFPAREGHKFMMNYLGIGCDAKVAYEFHMMRQEKPEKFCSQFVN 422

Query: 580 KVLYAREGARSIMDRSFADIPWQVRVEVDGVEVEVPEDAEGVLVTNIGSYMGGVDLWHNE 639
           K+ YA+EGAR IMDR+ AD+PWQV +EVDG ++E+P+D+EG++V NIGSYMGGVDLW N+
Sbjct: 423 KLRYAKEGARDIMDRACADLPWQVWLEVDGKDIEIPKDSEGLIVLNIGSYMGGVDLWQND 482

Query: 640 DETSDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARRLAQGKSIRIQLCAALPVQID 699
            E  DNF  Q MHDK LEVV + G WHLGKLQVGLS+ARRLAQGK IRI + +  PVQID
Sbjct: 483 YEHDDNFSIQCMHDKTLEVVCVRGAWHLGKLQVGLSQARRLAQGKVIRIHVSSPFPVQID 542

Query: 700 GEPWFQNPCTLVISHHGQAFMLKKAIEEPLGHAAAIMTDVLESAESNNVINTSQKRVLLQ 759
           GEP+ Q P  L I+HHGQ FML++A +EP GHAAAIM +VL  AE   VIN SQK+VLLQ
Sbjct: 543 GEPFIQQPGCLEITHHGQVFMLRRASDEPRGHAAAIMNEVLLDAECKGVINASQKKVLLQ 558

BLAST of Carg21706 vs. TAIR 10
Match: AT5G57690.1 (diacylglycerol kinase 4 )

HSP 1 Score: 139.4 bits (350), Expect = 1.2e-32
Identity = 114/390 (29.23%), Postives = 169/390 (43.33%), Query Frame = 0

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSLQVLMMFMNLMVMDCMLIF 420
           PL+V +N KSG R+G  +K+RL  L++  Q       +    +V         + C+   
Sbjct: 90  PLMVFVNPKSGGRQGPLIKERLQNLISEEQ-------VYDLTEVKPNEFIRYGLGCL--- 149

Query: 421 QVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDCIDK---QNFVSPPPVAILP 480
              E  + +G E    +  K+   +++V GGDGTVGWVL C+ +   QN +  PPV+I+P
Sbjct: 150 ---EAFASRGDECAKEIREKM---RIVVAGGDGTVGWVLGCLGELNLQNRLPVPPVSIMP 209

Query: 481 AGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGK------ 540
            GTGNDL+R   WGG       +  +   LH    A ++ LD W + I    G+      
Sbjct: 210 LGTGNDLSRSFGWGGSF-PFAWKSAIKRTLHRASVAPISRLDSWNILITMPSGEIVDPPY 269

Query: 541 QLKAPQ-----------------------FMNNYLGIGCDAKVALDIHNLREENPEKFYN 600
            LKA Q                          NY  IG DA+VA   H+LR E P     
Sbjct: 270 SLKATQECYIDQNLEIEGEIPPSTNGYEGVFYNYFSIGMDAQVAYGFHHLRNEKPYLANG 329

Query: 601 QFMNKVLYAREG-------ARSIMD---RSFADIPWQVRVEVDGVE---VEVPEDAEGVL 660
              NK++Y+  G          I D   R   +I      ++D  E   V VP+    V+
Sbjct: 330 PIANKIIYSGYGCSQGWFLTHCINDPGLRGLKNIMTLHIKKLDSSEWEKVPVPKSVRAVV 389

Query: 661 VTNIGSYMGGVDLWHNEDE---TSDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRARR 699
             N+ SY  G + W N  +       F      D +LE+  +   WH   + V L  A+ 
Sbjct: 390 ALNLHSYGSGRNPWGNLKQDYLEKRGFVEAQADDGLLEIFGLKQGWHASFVMVELISAKH 449

BLAST of Carg21706 vs. TAIR 10
Match: AT2G18730.1 (diacylglycerol kinase 3 )

HSP 1 Score: 132.1 bits (331), Expect = 1.9e-30
Identity = 105/391 (26.85%), Postives = 159/391 (40.66%), Query Frame = 0

Query: 361 PLLVLINKKSGARRGDSLKQRLNILLNPVQGNLNLLRILFSL-QVLMMFMNLMVMDCMLI 420
           P++V IN  SG R G  LK+RL  L++  Q        +F L +V         + C+  
Sbjct: 91  PMVVFINPNSGGRHGPVLKERLQQLMSEEQ--------VFDLTEVKPHEFVRYGLGCL-- 150

Query: 421 FQVFELSSEQGPESGLCLFRKVPHFKVLVCGGDGTVGWVLDC---IDKQNFVSPPPVAIL 480
               E  + +G E   C        +++V GGDGTVGWVL C   ++K      PPV ++
Sbjct: 151 ----EKVAAEGDE---CAKECRARLRIMVAGGDGTVGWVLGCLGELNKDGKSQIPPVGVI 210

Query: 481 PAGTGNDLARVLNWGGGLGSVERQGGLCTVLHHVENAAVTVLDRWKVAIVDQHGKQLKAP 540
           P GTGNDL+R   WGG      R   +   LH      V  LD WK+ +    G+ +  P
Sbjct: 211 PLGTGNDLSRSFGWGGSFPFAWR-SAVKRTLHRASMGPVARLDSWKILVSMPSGEVVDPP 270

Query: 541 QFMN-----------------------------NYLGIGCDAKVALDIHNLREENPEKFY 600
             +                              NYL IG DA+VA   H+LR   P    
Sbjct: 271 YSLKPAEENELDQGLDAGIDAPPLAKAYEGVFYNYLSIGMDAQVAYGFHHLRNTKPYLAQ 330

Query: 601 NQFMNKVLYAREGARS-------IMDRSFADIPWQVRVEVDGV------EVEVPEDAEGV 660
               NK++Y+  G          + D     +   +++ +  V      E+ VP++   +
Sbjct: 331 GPISNKIIYSSFGCSQGWFCTPCVNDPGLRGLRNIMKIHIKKVNCSQWEEIAVPKNVRSI 390

Query: 661 LVTNIGSYMGGVDLWHN---EDETSDNFDPQSMHDKVLEVVSISGTWHLGKLQVGLSRAR 699
           +  N+ SY  G   W N   +      F      D ++E+      WH   +   L  A+
Sbjct: 391 VALNLHSYGSGSHPWGNLKPDYLEKRGFVEAHCDDGLIEIFGFKQGWHASFVMAELISAK 450