Carg21682 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21682
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein of unknown function (DUF962)
Location	Carg_Chr01: 4484180 .. 4486657 (+)
RNA-Seq Expression	Carg21682
Synteny	Carg21682

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGCCAAAACCCTGCCATTCCACACATCGATTACACATATTTCGATGTGCCCTCCTGCTCTGGTTTTCGTAATCCCTTAGCTTTATCCCTCTTTTTAGTCCCATTCAATCTCCCACTTCTTTCTCACACCCATTTCTTTCTGTATTTCTCGTTTGAATTGGAAAAGTTCCGATGGGAAGGCGCGGATTGTTCGATCTCGAGAGGCATTTTGCATTCTATGGCGCTTATCATAGCAACCCAACTAATATTTTCATCCATATTCTCTTTGTTTGGCCGATTTTCTTCACTGCGCTCATGCTCTTCTACTACACCCCCTCTTTCTATTCTCTCCCCAAATGCCCTTGTGGGTTCAACACTGGCCTCGTTTTGAATTTTGGGTTTCTCTTTGCTTTCATTTATGGCGCTTTCTACGTACTTTTGGATAAGAGAGCTGGATCTTTGGCGGCTTTAATGTGTCTTCTCTGCTGGGTTGGAGCTAGTGTGTTCGCTTTTAAGCTTGGCTACACTCTTACATGGAAGGTGAACGCCATTCTTTCTTCTTTTGATTCCCGTTTGTTTGTTTTAATTCTTTCACAGATCTTTAAATAGCCCTATTTAGGTTGTCTGGTTTCTGCTTGAGTTTGAATATTTGAACCATTTATGTAACTCTGTTCCCTAGAGCTTTATTTAATTCTTCCCACTTTATGGTTGTTGTGGAATTTATTGCTCCTATTTATTTGTTGAATTATGTTGGCAAACCGTCTGTTCTTAAAGAACTAAGAACACATATGGTAGCTCTTATAAGGCCTTATTTCTTGTTCTTTTTTCTGATCAAAGTCTGTAAATAGATATCTCAGCTGTTGTTTAGACTTATTTTTACTGATCAGTTGCCCCACTCACAATTGTTACTGATCTTTAAATGTTGTAGCTAGAACTGAATAGACATCACCTTTCCATTTGTTTATCAAATTCGTTTAACGTCTGTGCTTATTATCAACACACTTTTCTAGCTATGTTGTTTGTAAAAATAAAAAAATGTTGGTTTCTGGCTGCAAGTAAAGTAAGTCCTGCCGCATGCCTAAGAAATAGTTGGGTTAGGTTCAAATGACACTTTAATTTGGCAGCAAGTCTTTTCTTGATTGGTTTATATTACAAAGATCTTCTTATAACACTGATTGATGTTGTTCTTCCTTCGACCCAACGTGTCCTTTTATCGTCCTCGTCCTCGTCCTCTTGATGCTGAACTAAGCTAAACTTAGATAGACATCAATTTTGAAGGGATTTTGAGCCTCTATCTGATGTACTTTTGTGAGAATTATCTCAAAGTTTTTGTGTTTGATCCCACGTCGGTTGGAGAGGGGAACGAAACATTTCTTATAAGGGTGTGGAAACCTTTCTGTTTTAAAAACCTTGAGAGGAAGCCCAAAATGGAAAATCCAAAGAGGACAATATATGCTATCGGTAGGCTTGGCCTGTGACTGATATTTGGGACGAGATAGGTTGCAGCTTTTGAGTGTTGGACCAGCTTGGAGATGAGATTCCTTTTAGATAACCTATGGCCACCTTTTTCCAAAAGTCTGCACCAGTACATAACATGATTGACATTGTATCCCTTTCTTTTTTTGATATCGTCATAACTTGATAGGCACAACCCTTTTCGAGTTTCTTCGAGGACTCGACGTTCTGTGATCCGCTCATTTCACATCTGTAAACTTTCGAGTACCACTTGGATCTATGTGACATGTTCAAATTATTGCAGAATTTATAGCTAAAGCTGGTTATTTTGTCATCTAATATATCAGGTAGTGTTGGCTGCACAGTTGTTCTGCTGGACAAGTCAGTTTATTGGCCATGGAGTATTCGAGGTAACTACTTAGTTAAACATCTCAAACCTTCAACTTGTTTTTCAAACTTAATCCCTCATGGACGTTGCTTTTTGGTTTCACTCGGTAGAAACGAGCACCCGCTCTGATCGACAACCTTACTCAAGCTTTTCTAATGGCTCCGTTCTTCGTTTTGTTAGAGGTAAGCTTTGAACAAACCCATTATGGTACTGTTGAACAGATCCTGTTCGACCTGTATAACAACGAAATATTAAAAAATGTTATTACTTCGTTATCTATTTACAGGTTCTTCAAGCTTTATTCGAATACGAACCGTATAAAGGTTTTCGTGCAAGCGTTAAGGCAAAGATAGAAGCGGAGAGGAAAGCTTGGCAAGACAAGAAAGCAAAAAAAAGCTCTTAGCTTGAATCTCTGTTCTAACCATATGCAGAGAATAATGTATACACATACGTATATGAACGAGTATTCGACACGTTGGTCGTAGCTTCGAATGTGTTTGAAAATTTCAGTGAACAGAGTTTGAATAAAATTAGAATGGCCCTCTTCTTACAATCCAAATCTGGGAATTGGTTTTACATTCTTGTGTTCATATGGAATGAAACGGTGGCGTATAAGTATGAGAATATTTTATTTTTGTGCATTTTTGCTAATAAAGT

mRNA sequence

TGCCAAAACCCTGCCATTCCACACATCGATTACACATATTTCGATGTGCCCTCCTGCTCTGGTTTTCGTAATCCCTTAGCTTTATCCCTCTTTTTAGTCCCATTCAATCTCCCACTTCTTTCTCACACCCATTTCTTTCTGTATTTCTCGTTTGAATTGGAAAAGTTCCGATGGGAAGGCGCGGATTGTTCGATCTCGAGAGGCATTTTGCATTCTATGGCGCTTATCATAGCAACCCAACTAATATTTTCATCCATATTCTCTTTGTTTGGCCGATTTTCTTCACTGCGCTCATGCTCTTCTACTACACCCCCTCTTTCTATTCTCTCCCCAAATGCCCTTGTGGGTTCAACACTGGCCTCGTTTTGAATTTTGGGTTTCTCTTTGCTTTCATTTATGGCGCTTTCTACGTACTTTTGGATAAGAGAGCTGGATCTTTGGCGGCTTTAATGTGTCTTCTCTGCTGGGTTGGAGCTAGTGTGTTCGCTTTTAAGCTTGGCTACACTCTTACATGGAAGGTAGTGTTGGCTGCACAGTTGTTCTGCTGGACAAGTCAGTTTATTGGCCATGGAGTATTCGAGAAACGAGCACCCGCTCTGATCGACAACCTTACTCAAGCTTTTCTAATGGCTCCGTTCTTCGTTTTGTTAGAGGTTCTTCAAGCTTTATTCGAATACGAACCGTATAAAGGTTTTCGTGCAAGCGTTAAGGCAAAGATAGAAGCGGAGAGGAAAGCTTGGCAAGACAAGAAAGCAAAAAAAAGCTCTTAGCTTGAATCTCTGTTCTAACCATATGCAGAGAATAATGTATACACATACGTATATGAACGAGTATTCGACACGTTGGTCGTAGCTTCGAATGTGTTTGAAAATTTCAGTGAACAGAGTTTGAATAAAATTAGAATGGCCCTCTTCTTACAATCCAAATCTGGGAATTGGTTTTACATTCTTGTGTTCATATGGAATGAAACGGTGGCGTATAAGTATGAGAATATTTTATTTTTGTGCATTTTTGCTAATAAAGT

Coding sequence (CDS)

ATGGGAAGGCGCGGATTGTTCGATCTCGAGAGGCATTTTGCATTCTATGGCGCTTATCATAGCAACCCAACTAATATTTTCATCCATATTCTCTTTGTTTGGCCGATTTTCTTCACTGCGCTCATGCTCTTCTACTACACCCCCTCTTTCTATTCTCTCCCCAAATGCCCTTGTGGGTTCAACACTGGCCTCGTTTTGAATTTTGGGTTTCTCTTTGCTTTCATTTATGGCGCTTTCTACGTACTTTTGGATAAGAGAGCTGGATCTTTGGCGGCTTTAATGTGTCTTCTCTGCTGGGTTGGAGCTAGTGTGTTCGCTTTTAAGCTTGGCTACACTCTTACATGGAAGGTAGTGTTGGCTGCACAGTTGTTCTGCTGGACAAGTCAGTTTATTGGCCATGGAGTATTCGAGAAACGAGCACCCGCTCTGATCGACAACCTTACTCAAGCTTTTCTAATGGCTCCGTTCTTCGTTTTGTTAGAGGTTCTTCAAGCTTTATTCGAATACGAACCGTATAAAGGTTTTCGTGCAAGCGTTAAGGCAAAGATAGAAGCGGAGAGGAAAGCTTGGCAAGACAAGAAAGCAAAAAAAAGCTCTTAG

Protein sequence

MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGFNTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLAAQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVKAKIEAERKAWQDKKAKKSS

Homology

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BLAST of Carg21682 vs. NCBI nr
Match: XP_022949543.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita moschata] >KAG6607458.1 hypothetical protein SDJN03_00800, partial [Cucurbita argyrosperma subsp. sororia] >KAG7037114.1 SPAC16E8.02 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 400.6 bits (1028), Expect = 7.9e-108
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAERKAWQDKKAKKSS
Sbjct: 181 AKIEAERKAWQDKKAKKSS 199

[Close]

BLAST of Carg21682 vs. NCBI nr
Match: XP_023525923.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 399.1 bits (1024), Expect = 2.3e-107
Identity = 198/199 (99.50%), Postives = 198/199 (99.50%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFAFIYG FYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFAFIYGTFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAERKAWQDKKAKKSS
Sbjct: 181 AKIEAERKAWQDKKAKKSS 199

[Close]

BLAST of Carg21682 vs. NCBI nr
Match: XP_022998807.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita maxima])

HSP 1 Score: 397.1 bits (1019), Expect = 8.8e-107
Identity = 197/199 (98.99%), Postives = 197/199 (98.99%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGF  SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFHTSVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAERKAWQDKKAKKSS
Sbjct: 181 AKIEAERKAWQDKKAKKSS 199

[Close]

BLAST of Carg21682 vs. NCBI nr
Match: XP_023519470.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 369.4 bits (947), Expect = 2.0e-98
Identity = 179/199 (89.95%), Postives = 187/199 (93.97%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYS PKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFA +Y  FY+L DKRAGSLAA MC LCWVGASVFAFKLG+TLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALVYAVFYILFDKRAGSLAAFMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNL QAFLMAPFFVLLE+LQALF+YEPY GF ASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQALFKYEPYNGFHASVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEA++KAW+ KK KKSS
Sbjct: 181 AKIEAKKKAWEGKKEKKSS 199

[Close]

BLAST of Carg21682 vs. NCBI nr
Match: XP_022924180.1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita moschata] >KAG6584470.1 hypothetical protein SDJN03_20402, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 367.5 bits (942), Expect = 7.4e-98
Identity = 178/199 (89.45%), Postives = 187/199 (93.97%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYS PKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFA +Y  FY+L DKRAGSLAA+MC LCWVGASVFAFKLG+TLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALVYAIFYILFDKRAGSLAAVMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNL QAFLMAPFFVLLE+LQA F+YEPY GF +SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHSSVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAE+KAW+ KK KKSS
Sbjct: 181 AKIEAEKKAWEGKKEKKSS 199

[Close]

BLAST of Carg21682 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 92.0 bits (227), Expect = 7.9e-18
Identity = 67/181 (37.02%), Postives = 91/181 (50.28%), Query Frame = 0


Query: 9   LERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGFNTGLVLNF 68
           L R ++FY AYHSNP NI IH + +  +  TAL+L +   +F          N+ L +N 
Sbjct: 4   LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLH---NFVITL-----INSKLQINV 63

Query: 69  GFLFAFIYGAFYVLLDKRAGSL-AALMCLLCWVGASVFAFKLGYTLTWKVVLAAQLFCWT 128
             L    Y  FYV LD   G L + ++ L  ++  S        +L  +      + CW 
Sbjct: 64  AHLVGLAYQIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWI 123

Query: 129 SQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVKAKIEAER 188
            QFIGHGVFEKR PAL+DNL Q+  +AP F  LE         P+ G+  SV +KI A  
Sbjct: 124 LQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSKIRANI 169

[Close]

BLAST of Carg21682 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 87.8 bits (216), Expect = 1.5e-16
Identity = 61/180 (33.89%), Postives = 87/180 (48.33%), Query Frame = 0


Query: 5   GLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGFNTGL 64
           GL DL     FY  YH NP N+ IH +FV  I F+   + +    + S            
Sbjct: 4   GLLDLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQS------------ 63

Query: 65  VLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLAAQLF 124
            ++   + + ++  FY LL    G LA ++ LL     ++        LT+K  L     
Sbjct: 64  -ISLTAVLSVLFSIFYCLLYLPTGLLAGVLLLL----LNLALIDHRVDLTFKQELGLFTI 123

Query: 125 CWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVKAKIE 184
            W  QF+GHGVFEKR PALIDNL Q+ ++AP+F++ E L  L       GF   +KA +E
Sbjct: 124 GWIFQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKL-------GFMPRLKATLE 159

[Close]

BLAST of Carg21682 vs. ExPASy TrEMBL
Match: A0A6J1GCD3 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita moschata OX=3662 GN=LOC111452859 PE=4 SV=1)

HSP 1 Score: 400.6 bits (1028), Expect = 3.8e-108
Identity = 199/199 (100.00%), Postives = 199/199 (100.00%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAERKAWQDKKAKKSS
Sbjct: 181 AKIEAERKAWQDKKAKKSS 199

[Close]

BLAST of Carg21682 vs. ExPASy TrEMBL
Match: A0A6J1K901 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita maxima OX=3661 GN=LOC111493360 PE=4 SV=1)

HSP 1 Score: 397.1 bits (1019), Expect = 4.3e-107
Identity = 197/199 (98.99%), Postives = 197/199 (98.99%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGF  SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFHTSVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAERKAWQDKKAKKSS
Sbjct: 181 AKIEAERKAWQDKKAKKSS 199

[Close]

BLAST of Carg21682 vs. ExPASy TrEMBL
Match: A0A6J1EE32 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita moschata OX=3662 GN=LOC111431702 PE=4 SV=1)

HSP 1 Score: 367.5 bits (942), Expect = 3.6e-98
Identity = 178/199 (89.45%), Postives = 187/199 (93.97%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYS PKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLVLNFGFLFA +Y  FY+L DKRAGSLAA+MC LCWVGASVFAFKLG+TLTWKVVLA
Sbjct: 61  NTGLVLNFGFLFALVYAIFYILFDKRAGSLAAVMCFLCWVGASVFAFKLGWTLTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNL QAFLMAPFFVLLE+LQA F+YEPY GF +SVK
Sbjct: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHSSVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAE+KAW+ KK KKSS
Sbjct: 181 AKIEAEKKAWEGKKEKKSS 199

[Close]

BLAST of Carg21682 vs. ExPASy TrEMBL
Match: A0A6J1C7C0 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Momordica charantia OX=3673 GN=LOC111009061 PE=4 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 8.9e-97
Identity = 174/199 (87.44%), Postives = 186/199 (93.47%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTN+FIHILFVWPIFFTALMLFY+TPSFYSLPKCPCGF
Sbjct: 1   MGRRGLFDLERHFAFYGAYHSNPTNVFIHILFVWPIFFTALMLFYFTPSFYSLPKCPCGF 60

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           N GLVLNFGFL A +YGAFY+LLDKR+GSLAA MC LCWVG SVFA+KLG+  TWKVVLA
Sbjct: 61  NHGLVLNFGFLLALVYGAFYILLDKRSGSLAAFMCFLCWVGGSVFAYKLGFDRTWKVVLA 120

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQL CWTSQF+GHG+FEKRAPALIDNL QAFLMAPFFVLLE+LQ LF+YEPYKGF ASVK
Sbjct: 121 AQLLCWTSQFLGHGLFEKRAPALIDNLAQAFLMAPFFVLLEILQGLFKYEPYKGFHASVK 180

Query: 181 AKIEAERKAWQDKKAKKSS 200
           AKIEAE+KAWQDKK KKSS
Sbjct: 181 AKIEAEKKAWQDKKEKKSS 199

[Close]

BLAST of Carg21682 vs. ExPASy TrEMBL
Match: A0A6J1KEF1 (uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495075 PE=4 SV=1)

HSP 1 Score: 360.1 bits (923), Expect = 5.8e-96
Identity = 175/198 (88.38%), Postives = 183/198 (92.42%), Query Frame = 0


Query: 1   MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGF 60
           MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYS PKCPCGF
Sbjct: 109 MGRRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSFPKCPCGF 168

Query: 61  NTGLVLNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKVVLA 120
           NTGLV+NFG LFA +Y  FYVL DKRAGSLAA MC LCWVGASV AFKLG+TLTWKVVLA
Sbjct: 169 NTGLVVNFGSLFALVYAVFYVLFDKRAGSLAAFMCFLCWVGASVLAFKLGWTLTWKVVLA 228

Query: 121 AQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRASVK 180
           AQLFCWTSQFIGHGVFEKRAPALIDNL QAFLMAPFFVLLE+LQA F+YEPY GF ASVK
Sbjct: 229 AQLFCWTSQFIGHGVFEKRAPALIDNLAQAFLMAPFFVLLEILQASFKYEPYNGFHASVK 288

Query: 181 AKIEAERKAWQDKKAKKS 199
           AK+EAE+KAW+ KK KKS
Sbjct: 289 AKMEAEKKAWEGKKEKKS 306

[Close]

BLAST of Carg21682 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 236.9 bits (603), Expect = 1.4e-62
Identity = 125/201 (62.19%), Postives = 151/201 (75.12%), Query Frame = 0


Query: 5   GLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFY-YTPSFYSLPKCPCGFNTG 64
           GLFDLE+HFAFYGAYHSNP NI IHI+FVWPIFF+ L+L +  TP F        GF+  
Sbjct: 6   GLFDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIF---DPSQLGFSQS 65

Query: 65  LVL------NFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWKV 124
           L L      N GF+FA IY  FY+ LDK++G +AALMC  CWVG+S  A +LG +L  KV
Sbjct: 66  LTLDGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSSLALKV 125

Query: 125 VLAAQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFRA 184
            LA+QL CWT QF+GHGVFEKRAPAL+DNL QAFLMAPFFVLLEVLQ++F YEPY GF+A
Sbjct: 126 GLASQLLCWTGQFVGHGVFEKRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQA 185

Query: 185 SVKAKIEAERKAWQDKKAKKS 199
            V AK+E++ K ++ KK KK+
Sbjct: 186 RVNAKVESDIKEYRAKKQKKN 203

[Close]

BLAST of Carg21682 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 222.2 bits (565), Expect = 3.6e-58
Identity = 115/201 (57.21%), Postives = 141/201 (70.15%), Query Frame = 0


Query: 3   RRGLFDLERHFAFYGAYHSNPTNIFIHILFVWPIFFTALMLFYYTPSFYSLPKCPCGFNT 62
           R GL DLE+HFAFYGAYHSNP NI IH LFVWP  F  L+  Y TP    L     GF  
Sbjct: 4   RIGLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPI--LDHSQLGFLK 63

Query: 63  GLV------LNFGFLFAFIYGAFYVLLDKRAGSLAALMCLLCWVGASVFAFKLGYTLTWK 122
            L       L+ GF     Y  FY+ LDK++G LAAL+C  CW+G+S  A +LG++LT K
Sbjct: 64  SLTFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLK 123

Query: 123 VVLAAQLFCWTSQFIGHGVFEKRAPALIDNLTQAFLMAPFFVLLEVLQALFEYEPYKGFR 182
           V +A+QL CWT QF+GHG+FEKRAPAL+DNL QAFLM PFFVLLEVLQ++F YEPY GF+
Sbjct: 124 VGVASQLLCWTGQFLGHGLFEKRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEPYPGFK 183

Query: 183 ASVKAKIEAERKAWQDKKAKK 198
           A V +KIE+  K W++KK ++
Sbjct: 184 ARVDSKIESGIKEWREKKKQQ 202

The following BLAST results are available for this feature:

BLAST of Carg21682 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022949543.1	7.9e-108	100.00	uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita m...	[more]
XP_023525923.1	2.3e-107	99.50	uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita p...	[more]
XP_022998807.1	8.8e-107	98.99	uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita m...	[more]
XP_023519470.1	2.0e-98	89.95	uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita p...	[more]
XP_022924180.1	7.4e-98	89.45	uncharacterized endoplasmic reticulum membrane protein YGL010W-like [Cucurbita m...	[more]

BLAST of Carg21682 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O13737	7.9e-18	37.02	2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97...	[more]
P25338	1.5e-16	33.89	2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC...	[more]

BLAST of Carg21682 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GCD3	3.8e-108	100.00	uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita...	[more]
A0A6J1K901	4.3e-107	98.99	uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita...	[more]
A0A6J1EE32	3.6e-98	89.45	uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Cucurbita...	[more]
A0A6J1C7C0	8.9e-97	87.44	uncharacterized endoplasmic reticulum membrane protein YGL010W-like OS=Momordica...	[more]
A0A6J1KEF1	5.8e-96	88.38	uncharacterized endoplasmic reticulum membrane protein YGL010W-like isoform X1 O...	[more]

BLAST of Carg21682 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G18720.1	1.4e-62	62.19	Protein of unknown function (DUF962)	[more]
AT1G74440.1	3.6e-58	57.21	Protein of unknown function (DUF962)	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009305	2-hydroxy-palmitic acid dioxygenase Mpo1-like	PFAM	PF06127	DUF962	coord: 8..165 e-value: 5.8E-31 score: 106.4
IPR009305	2-hydroxy-palmitic acid dioxygenase Mpo1-like	PANTHER	PTHR28026	DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)	coord: 1..196
None	No IPR available	PANTHER	PTHR28026:SF8	YGL010W-LIKE PROTEIN	coord: 1..196

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21682-RA	Carg21682-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046521	sphingoid catabolic process
cellular_component	GO:0005783	endoplasmic reticulum
cellular_component	GO:0016021	integral component of membrane