Carg21607 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21607
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Calcium-binding EF-hand family protein
Location	Carg_Chr06: 4029104 .. 4029547 (-)
RNA-Seq Expression	Carg21607
Synteny	Carg21607

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCAAAGATCTGAGCAATGATCAGAAATCCTCCATGAGAGAGGCCTTCACCCTCTTCGACACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATCCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTTACTGCTCCCTTTGATTTCAATCGCTTCCTCGAGATCATGTCCAAACACATGAAGCCCGAGTCCTTCGATCGCCAGCTTCGCGACGCCTTCAAGGTTCTCGACAAGGACAACACCGGCTACGTCCTGGTGTCCGAGCTCCGACACATTTTGACTAGTATAGGTGAGAAGCTGGAGCCGTCGGAGTTCGATGAGTGGATTCGAGAAGTGGAGGTTGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

mRNA sequence

ATGGGCAAAGATCTGAGCAATGATCAGAAATCCTCCATGAGAGAGGCCTTCACCCTCTTCGACACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATCCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTTACTGCTCCCTTTGATTTCAATCGCTTCCTCGAGATCATGTCCAAACACATGAAGCCCGAGTCCTTCGATCGCCAGCTTCGCGACGCCTTCAAGGTTCTCGACAAGGACAACACCGGCTACGTCCTGGTGTCCGAGCTCCGACACATTTTGACTAGTATAGGTGAGAAGCTGGAGCCGTCGGAGTTCGATGAGTGGATTCGAGAAGTGGAGGTTGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Coding sequence (CDS)

ATGGGCAAAGATCTGAGCAATGATCAGAAATCCTCCATGAGAGAGGCCTTCACCCTCTTCGACACCGACGGCGATGGCCGGATCGCCCCATCGGAGCTCGGGATCCTAATGCGTTCTCTCGGCGGCAACCCCACTCAAGCCCAACTCAAAGCCATTATCGCCGAGGAGAATCTTACTGCTCCCTTTGATTTCAATCGCTTCCTCGAGATCATGTCCAAACACATGAAGCCCGAGTCCTTCGATCGCCAGCTTCGCGACGCCTTCAAGGTTCTCGACAAGGACAACACCGGCTACGTCCTGGTGTCCGAGCTCCGACACATTTTGACTAGTATAGGTGAGAAGCTGGAGCCGTCGGAGTTCGATGAGTGGATTCGAGAAGTGGAGGTTGGACCGGATGGTAAAATTCGGTATGAGGATTTCATTGCTCGAATGGTGGCCAAATGA

Protein sequence

MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAPFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFDEWIREVEVGPDGKIRYEDFIARMVAK

Homology

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BLAST of Carg21607 vs. NCBI nr
Match: XP_023540891.1 (probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo] >KAG6596839.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. sororia] >KAG7028366.1 putative calcium-binding protein CML13, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 295.0 bits (754), Expect = 3.5e-76
Identity = 147/147 (100.00%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. NCBI nr
Match: XP_023005593.1 (probable calcium-binding protein CML13 [Cucurbita maxima])

HSP 1 Score: 293.9 bits (751), Expect = 7.7e-76
Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. NCBI nr
Match: XP_022943492.1 (probable calcium-binding protein CML13 [Cucurbita moschata])

HSP 1 Score: 292.7 bits (748), Expect = 1.7e-75
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFTRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. NCBI nr
Match: XP_038876882.1 (probable calcium-binding protein CML13 [Benincasa hispida])

HSP 1 Score: 287.0 bits (733), Expect = 9.5e-74
Identity = 143/147 (97.28%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLS+DQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPE FDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVG DG+IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGRIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. NCBI nr
Match: XP_022923443.1 (probable calcium-binding protein CML13 [Cucurbita moschata] >XP_022923444.1 probable calcium-binding protein CML13 [Cucurbita moschata] >XP_023553502.1 probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 282.0 bits (720), Expect = 3.0e-72
Identity = 140/147 (95.24%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLS++QKSSM+EAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMK E FDRQLRDAFKVLDK+NTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. ExPASy Swiss-Prot
Match: Q94AZ4 (Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 PE=2 SV=1)

HSP 1 Score: 250.0 bits (637), Expect = 1.7e-65
Identity = 122/148 (82.43%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LS+DQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K E FDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg21607 vs. ExPASy Swiss-Prot
Match: Q8VZ50 (Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 PE=2 SV=1)

HSP 1 Score: 245.4 bits (625), Expect = 4.1e-64
Identity = 119/148 (80.41%), Postives = 137/148 (92.57%), Query Frame = 0


Query: 1   MGKD-LSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LSNDQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K E FDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg21607 vs. ExPASy Swiss-Prot
Match: Q84VG0 (Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 GN=CML7 PE=2 SV=1)

HSP 1 Score: 230.7 bits (587), Expect = 1.1e-59
Identity = 110/146 (75.34%), Postives = 129/146 (88.36%), Query Frame = 0


Query: 2   GKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTAP 61
           GK+LS +Q +SMREAF+LFDTDGDGRIAPSELG+LMRSLGGNPTQAQL+ I A+E LTAP
Sbjct: 3   GKELSEEQVASMREAFSLFDTDGDGRIAPSELGVLMRSLGGNPTQAQLRDIAAQEKLTAP 62

Query: 62  FDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEFD 121
           FDF RFL++M  H++PE FDR LRDAF+VLDKD +G V V++LRH+LTSIGEKLEP EFD
Sbjct: 63  FDFPRFLDLMRAHLRPEPFDRPLRDAFRVLDKDASGTVSVADLRHVLTSIGEKLEPHEFD 122

Query: 122 EWIREVEVGPDGKIRYEDFIARMVAK 148
           EWIREV+V PDG IRY+DFI R+VAK
Sbjct: 123 EWIREVDVAPDGTIRYDDFIRRIVAK 148

[Close]

BLAST of Carg21607 vs. ExPASy Swiss-Prot
Match: P84339 (Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 2.6e-34
Identity = 71/149 (47.65%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   LS +Q S  +EAF+LFD DGDG I   ELG +MRSLG NP+QA+L+ +I E +   
Sbjct: 1   MADQLSEEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPSQAELEDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ M+    + ++++AFKV DKD  GY+  +ELRH++T++GEKL  S
Sbjct: 61  NGTIDFPEFLTMMARKMRDTDSEEEIKEAFKVFDKDGNGYISAAELRHVMTNLGEKLTDS 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M++K
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKMMLSK 149

[Close]

BLAST of Carg21607 vs. ExPASy Swiss-Prot
Match: P69097 (Calmodulin OS=Trypanosoma brucei brucei OX=5702 PE=3 SV=2)

HSP 1 Score: 146.4 bits (368), Expect = 2.6e-34
Identity = 70/149 (46.98%), Postives = 103/149 (69.13%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAE--ENL 60
           M   LSN+Q S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E  ++ 
Sbjct: 1   MADQLSNEQISEFKEAFSLFDKDGDGTITTKELGTVMRSLGQNPTEAELQDMINEVDQDG 60

Query: 61  TAPFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
           +   DF  FL +M++ M+    + ++++AF+V DKD  G++  +ELRHI+T++GEKL   
Sbjct: 61  SGTIDFPEFLTLMARKMQDSDSEEEIKEAFRVFDKDGNGFISAAELRHIMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M++K
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKMMMSK 149

[Close]

BLAST of Carg21607 vs. ExPASy TrEMBL
Match: A0A6J1L2L8 (probable calcium-binding protein CML13 OS=Cucurbita maxima OX=3661 GN=LOC111498532 PE=4 SV=1)

HSP 1 Score: 293.9 bits (751), Expect = 3.7e-76
Identity = 146/147 (99.32%), Postives = 147/147 (100.00%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. ExPASy TrEMBL
Match: A0A6J1FT67 (probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC111448243 PE=4 SV=1)

HSP 1 Score: 292.7 bits (748), Expect = 8.3e-76
Identity = 146/147 (99.32%), Postives = 146/147 (99.32%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDF RFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFTRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. ExPASy TrEMBL
Match: A0A6J1E9N4 (probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC111431138 PE=4 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.5e-72
Identity = 140/147 (95.24%), Postives = 145/147 (98.64%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLS++QKSSM+EAFTLFD+DGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA
Sbjct: 1   MGKDLSDEQKSSMKEAFTLFDSDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFLEIMSKHMK E FDRQLRDAFKVLDK+NTGYVLVSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLEIMSKHMKHEPFDRQLRDAFKVLDKENTGYVLVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVGPDGKIRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. ExPASy TrEMBL
Match: A0A0A0L2F2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 1.9e-72
Identity = 140/147 (95.24%), Postives = 143/147 (97.28%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLS+DQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPE FDRQLRDAFKVLDKDNTGYV VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGYVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVG DG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. ExPASy TrEMBL
Match: A0A5D3D7C6 (Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold443G001330 PE=4 SV=1)

HSP 1 Score: 280.0 bits (715), Expect = 5.6e-72
Identity = 139/147 (94.56%), Postives = 143/147 (97.28%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTA 60
           MGKDLS+DQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT+
Sbjct: 1   MGKDLSDDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLTS 60

Query: 61  PFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSEF 120
           PFDFNRFL+IMSKHMKPE FDRQLRDAFKVLDKDNTG+V VSELRHILTSIGEKLEPSEF
Sbjct: 61  PFDFNRFLDIMSKHMKPEPFDRQLRDAFKVLDKDNTGFVRVSELRHILTSIGEKLEPSEF 120

Query: 121 DEWIREVEVGPDGKIRYEDFIARMVAK 148
           DEWIREVEVG DG IRYEDFIARMVAK
Sbjct: 121 DEWIREVEVGSDGSIRYEDFIARMVAK 147

[Close]

BLAST of Carg21607 vs. TAIR 10
Match: AT1G12310.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 250.0 bits (637), Expect = 1.2e-66
Identity = 122/148 (82.43%), Postives = 139/148 (93.92%), Query Frame = 0


Query: 1   MGKD-LSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           MGKD LS+DQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPTQAQLK+IIA ENL+
Sbjct: 1   MGKDGLSDDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTQAQLKSIIASENLS 60

Query: 61  APFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K E FDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKLEP+E
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLEPNE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg21607 vs. TAIR 10
Match: AT1G62820.1 (Calcium-binding EF-hand family protein )

HSP 1 Score: 245.4 bits (625), Expect = 2.9e-65
Identity = 119/148 (80.41%), Postives = 137/148 (92.57%), Query Frame = 0


Query: 1   MGKD-LSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENLT 60
           M KD LSNDQ SSM+EAF LFDTDGDG+IAPSELGILMRSLGGNPT++QLK+II  ENL+
Sbjct: 1   MSKDGLSNDQVSSMKEAFMLFDTDGDGKIAPSELGILMRSLGGNPTESQLKSIITTENLS 60

Query: 61  APFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPSE 120
           +PFDFNRFL++M+KH+K E FDRQLRDAFKVLDK+ TG+V V++LRHILTSIGEKL+PSE
Sbjct: 61  SPFDFNRFLDLMAKHLKTEPFDRQLRDAFKVLDKEGTGFVAVADLRHILTSIGEKLQPSE 120

Query: 121 FDEWIREVEVGPDGKIRYEDFIARMVAK 148
           FDEWI+EV+VG DGKIRYEDFIARMVAK
Sbjct: 121 FDEWIKEVDVGSDGKIRYEDFIARMVAK 148

[Close]

BLAST of Carg21607 vs. TAIR 10
Match: AT5G21274.1 (calmodulin 6 )

HSP 1 Score: 146.0 bits (367), Expect = 2.4e-35
Identity = 71/149 (47.65%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L++DQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLSDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Carg21607 vs. TAIR 10
Match: AT3G43810.1 (calmodulin 7 )

HSP 1 Score: 145.6 bits (366), Expect = 3.2e-35
Identity = 71/149 (47.65%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L++DQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE IRE +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIREADVDGDGQINYEEFVKVMMAK 149

[Close]

BLAST of Carg21607 vs. TAIR 10
Match: AT2G41110.1 (calmodulin 2 )

HSP 1 Score: 144.4 bits (363), Expect = 7.1e-35
Identity = 70/149 (46.98%), Postives = 101/149 (67.79%), Query Frame = 0


Query: 1   MGKDLSNDQKSSMREAFTLFDTDGDGRIAPSELGILMRSLGGNPTQAQLKAIIAEENL-- 60
           M   L++DQ S  +EAF+LFD DGDG I   ELG +MRSLG NPT+A+L+ +I E +   
Sbjct: 1   MADQLTDDQISEFKEAFSLFDKDGDGCITTKELGTVMRSLGQNPTEAELQDMINEVDADG 60

Query: 61  TAPFDFNRFLEIMSKHMKPESFDRQLRDAFKVLDKDNTGYVLVSELRHILTSIGEKLEPS 120
               DF  FL +M++ MK    + +L++AF+V DKD  G++  +ELRH++T++GEKL   
Sbjct: 61  NGTIDFPEFLNLMARKMKDTDSEEELKEAFRVFDKDQNGFISAAELRHVMTNLGEKLTDE 120

Query: 121 EFDEWIREVEVGPDGKIRYEDFIARMVAK 148
           E DE I+E +V  DG+I YE+F+  M+AK
Sbjct: 121 EVDEMIKEADVDGDGQINYEEFVKVMMAK 149

The following BLAST results are available for this feature:

BLAST of Carg21607 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023540891.1	3.5e-76	100.00	probable calcium-binding protein CML13 [Cucurbita pepo subsp. pepo] >KAG6596839....	[more]
XP_023005593.1	7.7e-76	99.32	probable calcium-binding protein CML13 [Cucurbita maxima]	[more]
XP_022943492.1	1.7e-75	99.32	probable calcium-binding protein CML13 [Cucurbita moschata]	[more]
XP_038876882.1	9.5e-74	97.28	probable calcium-binding protein CML13 [Benincasa hispida]	[more]
XP_022923443.1	3.0e-72	95.24	probable calcium-binding protein CML13 [Cucurbita moschata] >XP_022923444.1 prob...	[more]

BLAST of Carg21607 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AZ4	1.7e-65	82.43	Probable calcium-binding protein CML13 OS=Arabidopsis thaliana OX=3702 GN=CML13 ...	[more]
Q8VZ50	4.1e-64	80.41	Probable calcium-binding protein CML14 OS=Arabidopsis thaliana OX=3702 GN=CML14 ...	[more]
Q84VG0	1.1e-59	75.34	Probable calcium-binding protein CML7 OS=Oryza sativa subsp. japonica OX=39947 G...	[more]
P84339	2.6e-34	47.65	Calmodulin OS=Agaricus bisporus OX=5341 PE=1 SV=2	[more]
P69097	2.6e-34	46.98	Calmodulin OS=Trypanosoma brucei brucei OX=5702 PE=3 SV=2	[more]

BLAST of Carg21607 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1L2L8	3.7e-76	99.32	probable calcium-binding protein CML13 OS=Cucurbita maxima OX=3661 GN=LOC1114985...	[more]
A0A6J1FT67	8.3e-76	99.32	probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC11144...	[more]
A0A6J1E9N4	1.5e-72	95.24	probable calcium-binding protein CML13 OS=Cucurbita moschata OX=3662 GN=LOC11143...	[more]
A0A0A0L2F2	1.9e-72	95.24	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G036390 PE=4 SV=1	[more]
A0A5D3D7C6	5.6e-72	94.56	Putative calcium-binding protein CML13 OS=Cucumis melo var. makuwa OX=1194695 GN...	[more]

BLAST of Carg21607 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G12310.1	1.2e-66	82.43	Calcium-binding EF-hand family protein	[more]
AT1G62820.1	2.9e-65	80.41	Calcium-binding EF-hand family protein	[more]
AT5G21274.1	2.4e-35	47.65	calmodulin 6	[more]
AT3G43810.1	3.2e-35	47.65	calmodulin 7	[more]
AT2G41110.1	7.1e-35	46.98	calmodulin 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002048	EF-hand domain	SMART	SM00054	efh_1	coord: 119..147 e-value: 6.9 score: 11.0 coord: 83..111 e-value: 4.7E-4 score: 29.5 coord: 12..40 e-value: 1.2E-4 score: 31.5
IPR002048	EF-hand domain	PFAM	PF13405	EF-hand_6	coord: 13..41 e-value: 2.1E-6 score: 27.1
IPR002048	EF-hand domain	PFAM	PF13499	EF-hand_7	coord: 82..144 e-value: 4.6E-13 score: 49.4
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 8..43 score: 12.979158
IPR002048	EF-hand domain	PROSITE	PS50222	EF_HAND_2	coord: 79..114 score: 12.449146
IPR002048	EF-hand domain	CDD	cd00051	EFh	coord: 14..72 e-value: 5.32189E-9 score: 47.5425
None	No IPR available	GENE3D	1.10.238.10		coord: 82..145 e-value: 1.1E-18 score: 69.1
None	No IPR available	GENE3D	1.10.238.10		coord: 3..81 e-value: 2.8E-20 score: 74.3
None	No IPR available	PANTHER	PTHR23050	CALCIUM BINDING PROTEIN	coord: 1..147
None	No IPR available	PANTHER	PTHR23050:SF412	SUBFAMILY NOT NAMED	coord: 1..147
IPR018247	EF-Hand 1, calcium-binding site	PROSITE	PS00018	EF_HAND_1	coord: 21..33
IPR011992	EF-hand domain pair	SUPERFAMILY	47473	EF-hand	coord: 5..144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21607-RA	Carg21607-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0005509	calcium ion binding