Carg21579 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21579
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Heavy metal-associated domain containing protein
Location	Carg_Chr18: 10137972 .. 10139463 (+)
RNA-Seq Expression	Carg21579
Synteny	Carg21579

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTTCTCCTTTATCGCAACCACTTAAACAAAGCTTTTCACAGTCCCATCATCCATGGCTCATAAAATCCTCCCCTGTTTCGCCGGCAGCGTTTAACCGGACCATGGCGGAGCGGCCCAACAAGACGGCGGCGGCGGCGGGAATTGGAGTTCGGAAGCTTAAGATTTTCCCTCATGCTTCTAGCCTTGCCTCCATCGAATCCTTATCTCTGCCTCTCGTCTCTGCTTTTTCTCTGTATCTCCGCCGTTTTTCGAGCTCTCCGGCTGAAATTAACGCTTAATGGTCCGTGATTCTTCAGGTTCAGGAAATCGTGTTCGCGGCGGACATTGGCTGCGCTGAATGCCAGAAGAAATTAGCGAATATTCTTGCCAAAATGAACGGTGAGTTTATGGGAATTTTGGGGCTCTGTTTCCTCGGAATCTTATTCCAATCTTCTAATTTCAATGGCCGATATTAGTCATTTCTAACATAGTATTAGAGTCATGCTCTTAATTTGGAAAGGTGTAGTCAATTTTAGACGAACACGACTCTCCACAATGGTATGATATTGTCCAGTTTGAGCATGAGTTTTCGTGGCTTTGCTTTGGGTTTACCCAAAATGCCTCCTGTCAATGGAGGGAGTATTTTTTGTTTATAAACTCATGATTATTTCCTAAATTAGCCGATGATGCGGGACTTCCATCATCCAACAGTCAAAAGTGACTAAAGTGTCGAACAAAGGGTGTACTTTGTTCGAAGACTCAAGATAAGGAGTCGAGCCCGAATAAAGGGAGATTGTTCAAGAGCTCCATCGACCTCAGGGAGGCTCTATGATGTAGTTTATTCGATGGGAGAATTGTTAGAGGGAGTCCACACCGGTAGTTTCTTCATTTGTGTGAGATTTTTTGGGGAAGTTTGAAGCAAATCCATGAGAGCTTATGGTCAAAATGGACAATATCATATCATTTGGTGTTCCTTTTTAGAACATGGGTTGTTTATAATTGGAAGTTCTTAATGATTTTTGTCGTGGATTTAGATACAGAGTCTGTGGTGGTGAATTTGCTGGACAAAAAAGTGATACTGACTCGAAAATTGCAGATTCCAACAAGTAGAGTTTCAACGATCAAGAGATTGCTTGGTTCTTCTTTCCGATAATGGTGAAGATATTGTGATATATAACAGAATATTTTCGGTCACATTCGTAGATAAATAAAGGATCATACTCTTTGTTGTTTAGTCTCGGCTATTATTGTTTTTATGAGAATATTAAATATTGGGTTCTGAATGCAACAAAATCATCGTACATTAAAAGATGATTTTGATCGGAGTTGAAAACATTTTAAAGGAGTGAGCCTTGCTGGAAAACATGGCTCTGGCTCAATGTAATTACGGGTTTAATCGATGGAGGCTCTGTTTGTGTCGTTTTCGTTATAATTCTTTTGACATTTTTGTTGTCTATTATTGGTGTATTGGTATGTGAGATTCCATATCGGTTTGAGAGGTAGTATGTACCTC

mRNA sequence

CTTCTCCTTTATCGCAACCACTTAAACAAAGCTTTTCACAGTCCCATCATCCATGGCTCATAAAATCCTCCCCTGTTTCGCCGGCAGCGTTTAACCGGACCATGGCGGAGCGGCCCAACAAGACGGCGGCGGCGGCGGGAATTGGAGTTCGGAAGCTTAAGATTTTCCCTCATGCTTCTAGCCTTGCCTCCATCGAATCCTTATCTCTGCCTCTCGTTCAGGAAATCGTGTTCGCGGCGGACATTGGCTGCGCTGAATGCCAGAAGAAATTAGCGAATATTCTTGCCAAAATGAACGATACAGAGTCTGTGGTGGTGAATTTGCTGGACAAAAAAGTGATACTGACTCGAAAATTGCAGATTCCAACAAGTAGAGTTTCAACGATCAAGAGATTGCTTGGTTCTTCTTTCCGATAATGGTGAAGATATTGTGATATATAACAGAATATTTTCGGTCACATTCGTAGATAAATAAAGGATCATACTCTTTGTTGTTTAGTCTCGGCTATTATTGTTTTTATGAGAATATTAAATATTGGGTTCTGAATGCAACAAAATCATCGTACATTAAAAGATGATTTTGATCGGAGTTGAAAACATTTTAAAGGAGTGAGCCTTGCTGGAAAACATGGCTCTGGCTCAATGTAATTACGGGTTTAATCGATGGAGGCTCTGTTTGTGTCGTTTTCGTTATAATTCTTTTGACATTTTTGTTGTCTATTATTGGTGTATTGGTATGTGAGATTCCATATCGGTTTGAGAGGTAGTATGTACCTC

Coding sequence (CDS)

ATGGCGGAGCGGCCCAACAAGACGGCGGCGGCGGCGGGAATTGGAGTTCGGAAGCTTAAGATTTTCCCTCATGCTTCTAGCCTTGCCTCCATCGAATCCTTATCTCTGCCTCTCGTTCAGGAAATCGTGTTCGCGGCGGACATTGGCTGCGCTGAATGCCAGAAGAAATTAGCGAATATTCTTGCCAAAATGAACGATACAGAGTCTGTGGTGGTGAATTTGCTGGACAAAAAAGTGATACTGACTCGAAAATTGCAGATTCCAACAAGTAGAGTTTCAACGATCAAGAGATTGCTTGGTTCTTCTTTCCGATAA

Protein sequence

MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR

Homology

[Close]

BLAST of Carg21579 vs. NCBI nr
Match: KAG7013033.1 (hypothetical protein SDJN02_25789 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 193.0 bits (489), Expect = 1.3e-45
Identity = 104/104 (100.00%), Postives = 104/104 (100.00%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60
           MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI
Sbjct: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60

Query: 61  LAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105
           LAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR
Sbjct: 61  LAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 104

[Close]

BLAST of Carg21579 vs. NCBI nr
Match: KAG6573976.1 (hypothetical protein SDJN03_27863, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 191.4 bits (485), Expect = 3.8e-45
Identity = 103/104 (99.04%), Postives = 103/104 (99.04%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60
           MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI
Sbjct: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60

Query: 61  LAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105
           LAKMNDTESVVVNLLDKKVILTRKLQIPTSR STIKRLLGSSFR
Sbjct: 61  LAKMNDTESVVVNLLDKKVILTRKLQIPTSRASTIKRLLGSSFR 104

[Close]

BLAST of Carg21579 vs. NCBI nr
Match: XP_023542320.1 (uncharacterized protein LOC111802252 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 186.4 bits (472), Expect = 1.2e-43
Identity = 103/105 (98.10%), Postives = 103/105 (98.10%), Query Frame = 0


Query: 1   MAERPNK-TAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLAN 60
           MAERPNK  AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLAN
Sbjct: 1   MAERPNKAAAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLAN 60

Query: 61  ILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105
           ILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR
Sbjct: 61  ILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105

[Close]

BLAST of Carg21579 vs. NCBI nr
Match: XP_022968296.1 (uncharacterized protein LOC111467567 isoform X3 [Cucurbita maxima])

HSP 1 Score: 181.8 bits (460), Expect = 3.0e-42
Identity = 99/105 (94.29%), Postives = 102/105 (97.14%), Query Frame = 0


Query: 1   MAERPNK-TAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLAN 60
           MAERPNK  AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVF ADIGCAECQKKLAN
Sbjct: 1   MAERPNKAAAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFTADIGCAECQKKLAN 60

Query: 61  ILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105
           IL+KMNDTESVVVNLLDKKVILTRKLQIP+SRVST+KRLLGSSFR
Sbjct: 61  ILSKMNDTESVVVNLLDKKVILTRKLQIPSSRVSTVKRLLGSSFR 105

[Close]

BLAST of Carg21579 vs. NCBI nr
Match: XP_022945539.1 (uncharacterized protein LOC111449743 isoform X3 [Cucurbita moschata])

HSP 1 Score: 181.8 bits (460), Expect = 3.0e-42
Identity = 100/103 (97.09%), Postives = 101/103 (98.06%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60
           MAERPNKT AAAGIGVRKLKIFPHASSLAS+ESLSLPLVQEIVFAADIGCAECQKKLANI
Sbjct: 1   MAERPNKT-AAAGIGVRKLKIFPHASSLASVESLSLPLVQEIVFAADIGCAECQKKLANI 60

Query: 61  LAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSF 104
           LAKMNDTESVVVNLLDKKVILTRKLQI TSRVSTIKRLLGSSF
Sbjct: 61  LAKMNDTESVVVNLLDKKVILTRKLQIQTSRVSTIKRLLGSSF 102

[Close]

BLAST of Carg21579 vs. ExPASy TrEMBL
Match: A0A6J1HUG9 (uncharacterized protein LOC111467567 isoform X3 OS=Cucurbita maxima OX=3661 GN=LOC111467567 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-42
Identity = 99/105 (94.29%), Postives = 102/105 (97.14%), Query Frame = 0


Query: 1   MAERPNK-TAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLAN 60
           MAERPNK  AAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVF ADIGCAECQKKLAN
Sbjct: 1   MAERPNKAAAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFTADIGCAECQKKLAN 60

Query: 61  ILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSFR 105
           IL+KMNDTESVVVNLLDKKVILTRKLQIP+SRVST+KRLLGSSFR
Sbjct: 61  ILSKMNDTESVVVNLLDKKVILTRKLQIPSSRVSTVKRLLGSSFR 105

[Close]

BLAST of Carg21579 vs. ExPASy TrEMBL
Match: A0A6J1G188 (uncharacterized protein LOC111449743 isoform X3 OS=Cucurbita moschata OX=3662 GN=LOC111449743 PE=4 SV=1)

HSP 1 Score: 181.8 bits (460), Expect = 1.5e-42
Identity = 100/103 (97.09%), Postives = 101/103 (98.06%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60
           MAERPNKT AAAGIGVRKLKIFPHASSLAS+ESLSLPLVQEIVFAADIGCAECQKKLANI
Sbjct: 1   MAERPNKT-AAAGIGVRKLKIFPHASSLASVESLSLPLVQEIVFAADIGCAECQKKLANI 60

Query: 61  LAKMNDTESVVVNLLDKKVILTRKLQIPTSRVSTIKRLLGSSF 104
           LAKMNDTESVVVNLLDKKVILTRKLQI TSRVSTIKRLLGSSF
Sbjct: 61  LAKMNDTESVVVNLLDKKVILTRKLQIQTSRVSTIKRLLGSSF 102

[Close]

BLAST of Carg21579 vs. ExPASy TrEMBL
Match: A0A6J1G176 (uncharacterized protein LOC111449743 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449743 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 3.7e-38
Identity = 100/130 (76.92%), Postives = 101/130 (77.69%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPL---------------------- 60
           MAERPNKT AAAGIGVRKLKIFPHASSLAS+ESLSLPL                      
Sbjct: 1   MAERPNKT-AAAGIGVRKLKIFPHASSLASVESLSLPLVSAFSLYLRRFSSSPAEINALW 60

Query: 61  -----VQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVS 104
                VQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTRKLQI TSRVS
Sbjct: 61  SVILQVQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTRKLQIQTSRVS 120

[Close]

BLAST of Carg21579 vs. ExPASy TrEMBL
Match: A0A6J1G168 (uncharacterized protein LOC111449743 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449743 PE=4 SV=1)

HSP 1 Score: 157.5 bits (397), Expect = 3.0e-35
Identity = 98/130 (75.38%), Postives = 99/130 (76.15%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPL---------------------- 60
           MAERPNKT AAAGIGVRKLKIFPHASSLAS+ESLSLPL                      
Sbjct: 1   MAERPNKT-AAAGIGVRKLKIFPHASSLASVESLSLPLVSAFSLYLRRFSSSPAEINALW 60

Query: 61  -----VQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTRKLQIPTSRVS 104
                VQEIVFAADIGCAECQKKLANILAKMN  ESVVVNLLDKKVILTRKLQI TSRVS
Sbjct: 61  SVILQVQEIVFAADIGCAECQKKLANILAKMN--ESVVVNLLDKKVILTRKLQIQTSRVS 120

[Close]

BLAST of Carg21579 vs. ExPASy TrEMBL
Match: A0A6J1DB71 (uncharacterized protein LOC111018707 OS=Momordica charantia OX=3673 GN=LOC111018707 PE=4 SV=1)

HSP 1 Score: 144.1 bits (362), Expect = 3.4e-31
Identity = 83/110 (75.45%), Postives = 92/110 (83.64%), Query Frame = 0


Query: 1   MAERPNKTAAAAGIGVRKLKIFPHASSLASIESLSLPLVQEIVFAADIGCAECQKKLANI 60
           MAER  K AA   IGVRK +IFPHASSLASIESLSLPLVQEIV  ADI C ECQ KLANI
Sbjct: 1   MAERAKKKAAV--IGVRKPRIFPHASSLASIESLSLPLVQEIVLTADIRCPECQNKLANI 60

Query: 61  LAKMNDTESVVVNLLDKKVILTRKLQIP------TSRVSTIKRLLGSSFR 105
           L+K+NDTESVVVNLL+KKVILTR+LQ+P      +S+V+TIKRLLGSSFR
Sbjct: 61  LSKINDTESVVVNLLEKKVILTRRLQVPSTCSNSSSKVATIKRLLGSSFR 108

[Close]

BLAST of Carg21579 vs. TAIR 10
Match: AT1G68585.1 (unknown protein; Has 23 Blast hits to 23 proteins in 6 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 23; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 70.5 bits (171), Expect = 9.2e-13
Identity = 40/85 (47.06%), Postives = 64/85 (75.29%), Query Frame = 0


Query: 26  SSLASIESLSLPLVQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILT--- 85
           +SLAS+ SLS+PL+QEIV +ADI C++CQ+K+A+I+A+M +T S++V++L+KKV LT   
Sbjct: 26  TSLASVASLSMPLIQEIVLSADIRCSDCQEKVADIMARMIETYSILVSVLEKKVTLTCTY 85

Query: 86  ---RKL-----QIPTSRVSTIKRLL 100
              R++     +    ++STIKRL+
Sbjct: 86  SGDRRVSKSYGEALLCKISTIKRLI 110

[Close]

BLAST of Carg21579 vs. TAIR 10
Match: AT2G35730.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 43.5 bits (101), Expect = 1.2e-04
Identity = 24/57 (42.11%), Postives = 35/57 (61.40%), Query Frame = 0


Query: 27 SLASIESLSLPLVQEIVFAADIGCAECQKKLANILAKMNDTESVVVNLLDKKVILTR 84
          +L   E LSLP  Q I   AD+GC  CQ +++ I++KM   E  VV+ L KK+++ R
Sbjct: 10 TLMLCEKLSLPSFQVIEINADVGCVACQDRVSKIVSKMTGIEEYVVD-LKKKLVMAR 65

The following BLAST results are available for this feature:

BLAST of Carg21579 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7013033.1	1.3e-45	100.00	hypothetical protein SDJN02_25789 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6573976.1	3.8e-45	99.04	hypothetical protein SDJN03_27863, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023542320.1	1.2e-43	98.10	uncharacterized protein LOC111802252 [Cucurbita pepo subsp. pepo]	[more]
XP_022968296.1	3.0e-42	94.29	uncharacterized protein LOC111467567 isoform X3 [Cucurbita maxima]	[more]
XP_022945539.1	3.0e-42	97.09	uncharacterized protein LOC111449743 isoform X3 [Cucurbita moschata]	[more]

BLAST of Carg21579 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg21579 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HUG9	1.5e-42	94.29	uncharacterized protein LOC111467567 isoform X3 OS=Cucurbita maxima OX=3661 GN=L...	[more]
A0A6J1G188	1.5e-42	97.09	uncharacterized protein LOC111449743 isoform X3 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1G176	3.7e-38	76.92	uncharacterized protein LOC111449743 isoform X1 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1G168	3.0e-35	75.38	uncharacterized protein LOC111449743 isoform X2 OS=Cucurbita moschata OX=3662 GN...	[more]
A0A6J1DB71	3.4e-31	75.45	uncharacterized protein LOC111018707 OS=Momordica charantia OX=3673 GN=LOC111018...	[more]

BLAST of Carg21579 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G68585.1	9.2e-13	47.06	unknown protein; Has 23 Blast hits to 23 proteins in 6 species: Archae - 0; Bact...	[more]
AT2G35730.1	1.2e-04	42.11	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 38..100 e-value: 8.9E-6 score: 27.9
None	No IPR available	PANTHER	PTHR47294	OS08G0431150 PROTEIN	coord: 25..86
None	No IPR available	PANTHER	PTHR47294:SF4	PROTEIN, PUTATIVE-RELATED	coord: 25..86
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 40..90

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21579-RA	Carg21579-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding