Carg21572 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21572
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionAlpha/beta-Hydrolases superfamily protein
LocationCarg_Chr18: 10174168 .. 10175208 (-)
RNA-Seq ExpressionCarg21572
SyntenyCarg21572
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCTACCATTAACCTAGGCCAGATTCCAAGTTTCGAAGAACATGTTAATGGCTTTGATCAACATGATGTGTTGGTTGAGGAGATTGAAGGTCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGAGCACAGATAGTTCCCTGTGTCTCTAATGCATTGCCGCCAGAGCTGGGTGTTACTTCTTGGGATGTAGTTGTGAATAAGCTGAGTAACATTTGGGCTCGTTTCTATATCCCAAAACAGTGCCAGGGGAAACTTCCTTTGCTTGTCTATTTCCATGGAGGTGGATTTTGTGTCGGCTCGGCAGCTTGGAGCTGCTACCATGAGTTCCTTGCCAAGGTATCTGCAAAAGCTGAGTGTATAATTATGTCTGTGAATTATCGTTTGGCACCAGAAAACCCTCTTCCTGCTCCTTATGAAGATGGATTAAAGACCATTTCATGGTTGAAACAAGTTGCTCTTATTGGGGGCAAGCAGAATTGGTGGTCAAGATACTGTGACTTTACCAGAATCTACCTTGGTGGTGATAGTGCGGGTGGCAACATAGCCTTCAATGTAGCCGCCAGACTTGGTGGTAAGACCACTGCTTCTGGAGCTGTGGTCTTGAAGCCGTTGGTTATTAGAGGTAACATTTTGATACAACCATTTTTTGGTGGGGAGTCCAGGACCAAATCAGAGAAGTTTCTAGTCCAACCTCCTCGTTCGGCACTGACGATGGCGGTTTCCGATACATACTGGCGGTTGGCATTGCCGTCTGGGGCAAACCGTGACCACCCTTGGTGCAATCCTTCGGCAAAAGGGCTGTTTAAGGTGGAGAATTTGAGGGTTTTGCCTAGTTTGGTATGTATATCGGAATTGGACATATTGAAAGATAGGAACTTGGAGTTCTGCTCTGCTTTACATAGAGAGGGTAAGCTGATCAATTATGTGGTTCATGAAGGAGTTGGCCATGCCTTTCAGGTTCTGAACAAATCACAACTCTCACAGACTCGGACTCTTGAAATGATTGGTCATATCAAGGATTTCTTATGTGTATAG

mRNA sequence

ATGCCTACCATTAACCTAGGCCAGATTCCAAGTTTCGAAGAACATGTTAATGGCTTTGATCAACATGATGTGTTGGTTGAGGAGATTGAAGGTCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGAGCACAGATAGTTCCCTGTGTCTCTAATGCATTGCCGCCAGAGCTGGGTGTTACTTCTTGGGATGTAGTTGTGAATAAGCTGAGTAACATTTGGGCTCGTTTCTATATCCCAAAACAGTGCCAGGGGAAACTTCCTTTGCTTGTCTATTTCCATGGAGGTGGATTTTGTGTCGGCTCGGCAGCTTGGAGCTGCTACCATGAGTTCCTTGCCAAGGTATCTGCAAAAGCTGAGTGTATAATTATGTCTGTGAATTATCGTTTGGCACCAGAAAACCCTCTTCCTGCTCCTTATGAAGATGGATTAAAGACCATTTCATGGTTGAAACAAGTTGCTCTTATTGGGGGCAAGCAGAATTGGTGGTCAAGATACTGTGACTTTACCAGAATCTACCTTGGTGGTGATAGTGCGGGTGGCAACATAGCCTTCAATGTAGCCGCCAGACTTGGTGGTAAGACCACTGCTTCTGGAGCTGTGGTCTTGAAGCCGTTGGTTATTAGAGGTAACATTTTGATACAACCATTTTTTGGTGGGGAGTCCAGGACCAAATCAGAGAAGTTTCTAGTCCAACCTCCTCGTTCGGCACTGACGATGGCGGTTTCCGATACATACTGGCGGTTGGCATTGCCGTCTGGGGCAAACCGTGACCACCCTTGGTGCAATCCTTCGGCAAAAGGGCTGTTTAAGGTGGAGAATTTGAGGGTTTTGCCTAGTTTGGTATGTATATCGGAATTGGACATATTGAAAGATAGGAACTTGGAGTTCTGCTCTGCTTTACATAGAGAGGGTAAGCTGATCAATTATGTGGTTCATGAAGGAGTTGGCCATGCCTTTCAGGTTCTGAACAAATCACAACTCTCACAGACTCGGACTCTTGAAATGATTGGTCATATCAAGGATTTCTTATGTGTATAG

Coding sequence (CDS)

ATGCCTACCATTAACCTAGGCCAGATTCCAAGTTTCGAAGAACATGTTAATGGCTTTGATCAACATGATGTGTTGGTTGAGGAGATTGAAGGTCTCATTAGAGTTTACGGAAATGGGTATGTGGAAAGAGCACAGATAGTTCCCTGTGTCTCTAATGCATTGCCGCCAGAGCTGGGTGTTACTTCTTGGGATGTAGTTGTGAATAAGCTGAGTAACATTTGGGCTCGTTTCTATATCCCAAAACAGTGCCAGGGGAAACTTCCTTTGCTTGTCTATTTCCATGGAGGTGGATTTTGTGTCGGCTCGGCAGCTTGGAGCTGCTACCATGAGTTCCTTGCCAAGGTATCTGCAAAAGCTGAGTGTATAATTATGTCTGTGAATTATCGTTTGGCACCAGAAAACCCTCTTCCTGCTCCTTATGAAGATGGATTAAAGACCATTTCATGGTTGAAACAAGTTGCTCTTATTGGGGGCAAGCAGAATTGGTGGTCAAGATACTGTGACTTTACCAGAATCTACCTTGGTGGTGATAGTGCGGGTGGCAACATAGCCTTCAATGTAGCCGCCAGACTTGGTGGTAAGACCACTGCTTCTGGAGCTGTGGTCTTGAAGCCGTTGGTTATTAGAGGTAACATTTTGATACAACCATTTTTTGGTGGGGAGTCCAGGACCAAATCAGAGAAGTTTCTAGTCCAACCTCCTCGTTCGGCACTGACGATGGCGGTTTCCGATACATACTGGCGGTTGGCATTGCCGTCTGGGGCAAACCGTGACCACCCTTGGTGCAATCCTTCGGCAAAAGGGCTGTTTAAGGTGGAGAATTTGAGGGTTTTGCCTAGTTTGGTATGTATATCGGAATTGGACATATTGAAAGATAGGAACTTGGAGTTCTGCTCTGCTTTACATAGAGAGGGTAAGCTGATCAATTATGTGGTTCATGAAGGAGTTGGCCATGCCTTTCAGGTTCTGAACAAATCACAACTCTCACAGACTCGGACTCTTGAAATGATTGGTCATATCAAGGATTTCTTATGTGTATAG

Protein sequence

MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV
Homology
BLAST of Carg21572 vs. NCBI nr
Match: XP_022944859.1 (probable carboxylesterase 6 [Cucurbita moschata] >KAG7013040.1 putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 724.5 bits (1869), Expect = 4.2e-205
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Carg21572 vs. NCBI nr
Match: KAG6573982.1 (putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 723.4 bits (1866), Expect = 9.3e-205
Identity = 345/346 (99.71%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYV+RAQIVPCVSNALPPELGV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVDRAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Carg21572 vs. NCBI nr
Match: XP_022968602.1 (probable carboxylesterase 6 [Cucurbita maxima])

HSP 1 Score: 721.1 bits (1860), Expect = 4.6e-204
Identity = 344/346 (99.42%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINLGQIPSFEEHVNGFDQHD+LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDMLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALP GANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPFGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Carg21572 vs. NCBI nr
Match: XP_023542364.1 (probable carboxylesterase 6 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 711.1 bits (1834), Expect = 4.8e-201
Identity = 341/346 (98.55%), Postives = 341/346 (98.55%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINLGQIPSFEEHVNG DQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPEL V
Sbjct: 1   MPTINLGQIPSFEEHVNGSDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELDV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLK ISWLKQVAL GGKQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKAISWLKQVALNGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIK FLCV
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKAFLCV 346

BLAST of Carg21572 vs. NCBI nr
Match: XP_038893358.1 (probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893360.1 probable carboxylesterase 6 [Benincasa hispida] >XP_038893361.1 probable carboxylesterase 6 [Benincasa hispida])

HSP 1 Score: 676.4 bits (1744), Expect = 1.3e-190
Identity = 317/346 (91.62%), Postives = 333/346 (96.24%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINL QIPSFEEH NGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPELGV
Sbjct: 6   MPTINLDQIPSFEEHGNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVV++KL+NIWARFYIP QCQ KLPL+VYFHGGGFCVGSAAWSCYHEFLAK+SAKA+
Sbjct: 66  TSWDVVMDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAD 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+ WLKQVA +G KQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGAKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVI+G+ILIQPFFGGESRTKSEKFLVQPPRS LT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVVLKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALPSG NRDHPWCNPSAKGLFKVE+LRVLPSL+CISE+DILKDRNLEFCSA
Sbjct: 246 AVSDTYWRLALPSGTNRDHPWCNPSAKGLFKVEDLRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHR GKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIKDFLCV
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKDFLCV 351

BLAST of Carg21572 vs. ExPASy Swiss-Prot
Match: Q9SX25 (Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1)

HSP 1 Score: 357.5 bits (916), Expect = 1.8e-97
Identity = 173/332 (52.11%), Postives = 230/332 (69.28%), Query Frame = 0

Query: 17  NGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSWDVVVNKLSNIWAR 76
           N  + H  +V+E+EGLI+VY +G+VER+Q++PCV  +LP ELGVT  DVV++KL+N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 77  FYIP----KQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAP 136
            Y+P    K    KLPL+VYFHGGGFCVGSA+W CYHEFLA++SA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 137 ENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAARLG 196
           ENPLPA YEDG+  I WL +        N W++ CDF RI+L GDSAGGNIA  VAARL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNKAR----NDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 197 GKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALP 256
                +       L I G ILIQPF+ GE RT+SE+ +     + LT+A SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 257 SGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHREGKLINYVV 316
            GANR+HP+C P       +++  V  +LVC++E+D+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDG---NEDVIKRVL 314

Query: 317 HEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFL 345
           H+GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Carg21572 vs. ExPASy Swiss-Prot
Match: Q9LFR7 (Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1)

HSP 1 Score: 327.0 bits (837), Expect = 2.5e-88
Identity = 167/331 (50.45%), Postives = 220/331 (66.47%), Query Frame = 0

Query: 21  QHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSWDVVVNKLSN-IWARFYI 80
           +H  +VEEIEGLI+V+ +G VER  IVP VS  + P    T++D+   KLSN  W R YI
Sbjct: 24  RHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDI---KLSNDTWTRVYI 83

Query: 81  PKQCQGK----LPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENP 140
           P          LPLLVYFHGGGFCVGSAAWSCYH+FL  ++ KA C+I+SVNYRLAPE+ 
Sbjct: 84  PDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHR 143

Query: 141 LPAPYEDGLKTISWL--KQVALIGGKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAARLGG 200
           LPA Y+DG+  +SWL  +Q++  GG  +W S+ C+ + ++L GDSAG NIA+ VA R+  
Sbjct: 144 LPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVRI-- 203

Query: 201 KTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPS 260
              ASG      L ++G ILI PFFGGESRT SEK       SALT++ SD YWRLALP 
Sbjct: 204 --MASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPR 263

Query: 261 GANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHREGKLINYVVH 320
           GA+RDHPWCNP    L      ++  ++V ++E DILK+RNLE C  +   GK +  +VH
Sbjct: 264 GASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVH 323

Query: 321 EGVGHAFQVLNKSQLSQTRTLEMIGHIKDFL 345
            GVGHAF +L+ S +S+ R  +M+  + +F+
Sbjct: 324 GGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Carg21572 vs. ExPASy Swiss-Prot
Match: Q9FG13 (Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.5e-53
Identity = 112/321 (34.89%), Postives = 182/321 (56.70%), Query Frame = 0

Query: 25  LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSW--DVVVNKLSNIWARFYIPKQ 84
           + E+  GL+++  NG V R++ +  ++  +P +   T    D + +K +N+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 85  CQGK--LPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENPLPAPY 144
              +  LP++V+FHGGGFC GS +W  +H F   +++    +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 145 EDGLKTISWLKQVALIGGKQNWW--SRYCDFTRIYLGGDSAGGNIAFNVAARLGGKTTAS 204
           ED    ++WL   A+  G  +W+      DF R+++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 205 GAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPSGANRD 264
           G++ L P+ +RG +L+ PFFGGE RT SE     P  + L++ + D +WRL+LP+GA RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 265 HPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHR-EGKLINYVVHEGVG 324
           H   NP       +E++ + P LV +   ++L+DR  E+   L +  GK ++Y+  E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 325 HAFQVLNKSQLSQTRTLEMIG 339
           H F     S  +  + L +IG
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIG 322

BLAST of Carg21572 vs. ExPASy Swiss-Prot
Match: Q0ZPV7 (Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 1.1e-43
Identity = 103/280 (36.79%), Postives = 158/280 (56.43%), Query Frame = 0

Query: 60  VTSWDVVVNKLSNIWARFYIPKQC---QGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVS 119
           V + D+ +N L N + R ++P+       KLPL+VYFHGGGF + SAA + +H+F  +++
Sbjct: 53  VLTKDLALNPLHNTFVRLFLPRHALYNSAKLPLVVYFHGGGFILFSAASTIFHDFCCEMA 112

Query: 120 AKAECIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGG 179
             A  +I SV+YRLAPE+ LPA Y+D ++ + W+K       +  W + + DF+  ++ G
Sbjct: 113 VHAGVVIASVDYRLAPEHRLPAAYDDAMEALQWIKD-----SRDEWLTNFADFSNCFIMG 172

Query: 180 DSAGGNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRS 239
           +SAGGNIA++   R      A+ A  L PL I+G +L +P FGG  RT SE  L    R 
Sbjct: 173 ESAGGNIAYHAGLR-----AAAVADELLPLKIKGLVLDEPGFGGSKRTGSELRLANDSR- 232

Query: 240 ALTMAVSDTYWRLALPSGANRDHPWCNPSAKG--LFKVENLRVLPSLVCI--SELDILKD 299
            L   V D  W L+LP GA+RDH +CNP+A+   L+  + +R L   V +     D + D
Sbjct: 233 -LPTFVLDLIWELSLPMGADRDHEYCNPTAESEPLYSFDKIRSLGWRVMVVGCHGDPMID 292

Query: 300 RNLEFCSALHREGKLINYVVHEGVG--HAFQVLNKSQLSQ 331
           R +E    L ++G  ++ V    VG  HA ++ +  +  Q
Sbjct: 293 RQMELAERLEKKG--VDVVAQFDVGGYHAVKLEDPEKAKQ 318

BLAST of Carg21572 vs. ExPASy Swiss-Prot
Match: Q5NUF4 (2-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 3.0e-41
Identity = 112/329 (34.04%), Postives = 164/329 (49.85%), Query Frame = 0

Query: 28  EIEGLIRVYGNGYVER---AQIVPCVSNALPPELGVTSWDVVVNKLSNIWARFYIPK--Q 87
           E+  L+RVY +G VER   +  VP   +   PE GV++ D+V+++   I AR Y+PK   
Sbjct: 16  ELPPLLRVYKDGTVERFLGSSFVP--PSPEDPETGVSTKDIVISENPTISARVYLPKLNN 75

Query: 88  CQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENPLPAPYED 147
              KLP+LVY+HGG FC+ SA    +  +L  V++KA  +++S+ YRLAPE+PLPA YED
Sbjct: 76  TTEKLPILVYYHGGAFCLESAFSFLHQRYLNIVASKANVLVVSIEYRLAPEHPLPAAYED 135

Query: 148 GLKTISWLKQVALIGGKQN----WWSRYCDFTRIYLGGDSAGGNIAFNVAARLGGKTTAS 207
           G   + W+   +    K      W  ++ DF R Y+GGD++G NIA N A R+G +    
Sbjct: 136 GWYALKWVTSHSTNNNKPTNADPWLIKHGDFNRFYIGGDTSGANIAHNAALRVGAEALPG 195

Query: 208 GAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPSG-ANR 267
           G      L I G +   P F G     SE   V+    +  M V    W    P      
Sbjct: 196 G------LRIAGVLSAFPLFWGSKPVLSEP--VEGHEKSSPMQV----WNFVYPDAPGGI 255

Query: 268 DHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHREGKL--INYVVHEG 327
           D+P  NP A G   +  L     LV ++  D L+DR + +  A+   G    +    +EG
Sbjct: 256 DNPLINPLAPGAPNLATLGCPKMLVFVAGKDDLRDRGIWYYEAVKESGWKGDVELAQYEG 315

Query: 328 VGHAFQVLNKSQLSQTRTLEMIGHIKDFL 345
             H FQ+ +        + ++IG I  FL
Sbjct: 316 EEHCFQIYHP---ETENSKDLIGRIASFL 327

BLAST of Carg21572 vs. ExPASy TrEMBL
Match: A0A6J1FZC1 (probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111449259 PE=4 SV=1)

HSP 1 Score: 724.5 bits (1869), Expect = 2.0e-205
Identity = 346/346 (100.00%), Postives = 346/346 (100.00%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Carg21572 vs. ExPASy TrEMBL
Match: A0A6J1HTY6 (probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111467774 PE=4 SV=1)

HSP 1 Score: 721.1 bits (1860), Expect = 2.2e-204
Identity = 344/346 (99.42%), Postives = 345/346 (99.71%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINLGQIPSFEEHVNGFDQHD+LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV
Sbjct: 1   MPTINLGQIPSFEEHVNGFDQHDMLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE
Sbjct: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG
Sbjct: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM
Sbjct: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
           AVSDTYWRLALP GANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA
Sbjct: 241 AVSDTYWRLALPFGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV
Sbjct: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 346

BLAST of Carg21572 vs. ExPASy TrEMBL
Match: A0A0A0KUG5 (Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G608500 PE=4 SV=1)

HSP 1 Score: 672.9 bits (1735), Expect = 7.0e-190
Identity = 315/346 (91.04%), Postives = 330/346 (95.38%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINL QIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPELGV
Sbjct: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVV+KL+NIWARFYIP QCQ KLPL+VYFHGGGFCVGSAAWSCYHEFLAK+SAKA 
Sbjct: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKAN 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CIIMSVNYRLAPENPLPAPYEDGLKT+ WLKQVA +GGKQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIMSVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAV+LKPLVI+G+ILIQPFFGGESRTKSEKFLVQPPRS LT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTKSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VE+LRVLPSL+CISE+DILKDRNLEFCSA
Sbjct: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDLRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHR GKLINYVV+EGVGHAFQVLNKSQLSQTRTLEMI HIK FLCV
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMIDHIKAFLCV 351

BLAST of Carg21572 vs. ExPASy TrEMBL
Match: A0A1S3BFC8 (probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.7e-188
Identity = 311/346 (89.88%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINL QIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPELGV
Sbjct: 1   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 60

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVV+KL+NIWARFYIP QCQ KLPL+VYFHGGGFCVGSAAWSCYHEFLAK+SAK++
Sbjct: 61  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 120

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CII+SVNYRLAPENPLPAPYEDGLKT+ WLKQVA +GGKQNWWSRYCDFT+IYL GDSAG
Sbjct: 121 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 180

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAV+LKPLVI+G+ILIQPFFGGESRT SEKFLVQPPRS LT+
Sbjct: 181 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 240

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VE+ RVLPSL+CISE+DILKDRNLEFCSA
Sbjct: 241 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 300

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHR GKLINYVV+EGVGHAFQVLNKSQLSQTRTLEM  HIKDFLCV
Sbjct: 301 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 346

BLAST of Carg21572 vs. ExPASy TrEMBL
Match: A0A5A7SV76 (Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold475G00820 PE=4 SV=1)

HSP 1 Score: 668.3 bits (1723), Expect = 1.7e-188
Identity = 311/346 (89.88%), Postives = 329/346 (95.09%), Query Frame = 0

Query: 1   MPTINLGQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGV 60
           MPTINL QIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVER QIVPCVSNALPPELGV
Sbjct: 6   MPTINLDQIPSFEEHVNGFDQHDVLVEEIEGLIRVYGNGYVERPQIVPCVSNALPPELGV 65

Query: 61  TSWDVVVNKLSNIWARFYIPKQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAE 120
           TSWDVVV+KL+NIWARFYIP QCQ KLPL+VYFHGGGFCVGSAAWSCYHEFLAK+SAK++
Sbjct: 66  TSWDVVVDKLNNIWARFYIPTQCQEKLPLIVYFHGGGFCVGSAAWSCYHEFLAKLSAKSD 125

Query: 121 CIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAG 180
           CII+SVNYRLAPENPLPAPYEDGLKT+ WLKQVA +GGKQNWWSRYCDFT+IYL GDSAG
Sbjct: 126 CIIISVNYRLAPENPLPAPYEDGLKTLQWLKQVAFVGGKQNWWSRYCDFTKIYLSGDSAG 185

Query: 181 GNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTM 240
           GNIAFNVAARLGGKTTASGAV+LKPLVI+G+ILIQPFFGGESRT SEKFLVQPPRS LT+
Sbjct: 186 GNIAFNVAARLGGKTTASGAVILKPLVIKGSILIQPFFGGESRTNSEKFLVQPPRSPLTL 245

Query: 241 AVSDTYWRLALPSGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSA 300
            VSDTYWRLALPSG NRDHPWCNPS KGLF VE+ RVLPSL+CISE+DILKDRNLEFCSA
Sbjct: 246 GVSDTYWRLALPSGTNRDHPWCNPSTKGLFTVEDSRVLPSLICISEMDILKDRNLEFCSA 305

Query: 301 LHREGKLINYVVHEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFLCV 347
           LHR GKLINYVV+EGVGHAFQVLNKSQLSQTRTLEM  HIKDFLCV
Sbjct: 306 LHRAGKLINYVVYEGVGHAFQVLNKSQLSQTRTLEMFDHIKDFLCV 351

BLAST of Carg21572 vs. TAIR 10
Match: AT1G68620.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 357.5 bits (916), Expect = 1.2e-98
Identity = 173/332 (52.11%), Postives = 230/332 (69.28%), Query Frame = 0

Query: 17  NGFDQHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSWDVVVNKLSNIWAR 76
           N  + H  +V+E+EGLI+VY +G+VER+Q++PCV  +LP ELGVT  DVV++KL+N+WAR
Sbjct: 15  NNSNIHGPVVDEVEGLIKVYKDGHVERSQLLPCVDPSLPLELGVTCSDVVIDKLTNVWAR 74

Query: 77  FYIP----KQCQGKLPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAP 136
            Y+P    K    KLPL+VYFHGGGFCVGSA+W CYHEFLA++SA++ C++MSVNYRLAP
Sbjct: 75  LYVPMTTTKSSVSKLPLIVYFHGGGFCVGSASWLCYHEFLARLSARSRCLVMSVNYRLAP 134

Query: 137 ENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAARLG 196
           ENPLPA YEDG+  I WL +        N W++ CDF RI+L GDSAGGNIA  VAARL 
Sbjct: 135 ENPLPAAYEDGVNAILWLNKAR----NDNLWAKQCDFGRIFLAGDSAGGNIAQQVAARLA 194

Query: 197 GKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALP 256
                +       L I G ILIQPF+ GE RT+SE+ +     + LT+A SD +WR++LP
Sbjct: 195 SPEDLA-------LKIEGTILIQPFYSGEERTESERRVGNDKTAVLTLASSDAWWRMSLP 254

Query: 257 SGANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHREGKLINYVV 316
            GANR+HP+C P       +++  V  +LVC++E+D+L D N+E C        +I  V+
Sbjct: 255 RGANREHPYCKPVK---MIIKSSTVTRTLVCVAEMDLLMDSNMEMCDG---NEDVIKRVL 314

Query: 317 HEGVGHAFQVLNKSQLSQTRTLEMIGHIKDFL 345
           H+GVGHAF +L KSQL+ T TLEM+  I  F+
Sbjct: 315 HKGVGHAFHILGKSQLAHTTTLEMLCQIDAFI 329

BLAST of Carg21572 vs. TAIR 10
Match: AT5G16080.1 (carboxyesterase 17 )

HSP 1 Score: 327.0 bits (837), Expect = 1.8e-89
Identity = 167/331 (50.45%), Postives = 220/331 (66.47%), Query Frame = 0

Query: 21  QHDVLVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSWDVVVNKLSN-IWARFYI 80
           +H  +VEEIEGLI+V+ +G VER  IVP VS  + P    T++D+   KLSN  W R YI
Sbjct: 24  RHGPVVEEIEGLIKVFNDGCVERPPIVPIVSPTIHPSSKATAFDI---KLSNDTWTRVYI 83

Query: 81  PKQCQGK----LPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENP 140
           P          LPLLVYFHGGGFCVGSAAWSCYH+FL  ++ KA C+I+SVNYRLAPE+ 
Sbjct: 84  PDAAAASPSVTLPLLVYFHGGGFCVGSAAWSCYHDFLTSLAVKARCVIVSVNYRLAPEHR 143

Query: 141 LPAPYEDGLKTISWL--KQVALIGGKQNWWSRYCDFTRIYLGGDSAGGNIAFNVAARLGG 200
           LPA Y+DG+  +SWL  +Q++  GG  +W S+ C+ + ++L GDSAG NIA+ VA R+  
Sbjct: 144 LPAAYDDGVNVVSWLVKQQISTGGGYPSWLSK-CNLSNVFLAGDSAGANIAYQVAVRI-- 203

Query: 201 KTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPS 260
              ASG      L ++G ILI PFFGGESRT SEK       SALT++ SD YWRLALP 
Sbjct: 204 --MASGKYA-NTLHLKGIILIHPFFGGESRTSSEKQQHHTKSSALTLSASDAYWRLALPR 263

Query: 261 GANRDHPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHREGKLINYVVH 320
           GA+RDHPWCNP    L      ++  ++V ++E DILK+RNLE C  +   GK +  +VH
Sbjct: 264 GASRDHPWCNP----LMSSAGAKLPTTMVFMAEFDILKERNLEMCKVMRSHGKRVEGIVH 323

Query: 321 EGVGHAFQVLNKSQLSQTRTLEMIGHIKDFL 345
            GVGHAF +L+ S +S+ R  +M+  + +F+
Sbjct: 324 GGVGHAFHILDNSSVSRDRIHDMMCRLHNFI 341

BLAST of Carg21572 vs. TAIR 10
Match: AT5G06570.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-54
Identity = 112/321 (34.89%), Postives = 182/321 (56.70%), Query Frame = 0

Query: 25  LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSW--DVVVNKLSNIWARFYIPKQ 84
           + E+  GL+++  NG V R++ +  ++  +P +   T    D + +K +N+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 85  CQGK--LPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENPLPAPY 144
              +  LP++V+FHGGGFC GS +W  +H F   +++    +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 145 EDGLKTISWLKQVALIGGKQNWW--SRYCDFTRIYLGGDSAGGNIAFNVAARLGGKTTAS 204
           ED    ++WL   A+  G  +W+      DF R+++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 205 GAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPSGANRD 264
           G++ L P+ +RG +L+ PFFGGE RT SE     P  + L++ + D +WRL+LP+GA RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 265 HPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHR-EGKLINYVVHEGVG 324
           H   NP       +E++ + P LV +   ++L+DR  E+   L +  GK ++Y+  E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 325 HAFQVLNKSQLSQTRTLEMIG 339
           H F     S  +  + L +IG
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIG 322

BLAST of Carg21572 vs. TAIR 10
Match: AT5G06570.2 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 210.3 bits (534), Expect = 2.5e-54
Identity = 112/321 (34.89%), Postives = 182/321 (56.70%), Query Frame = 0

Query: 25  LVEEIEGLIRVYGNGYVERAQIVPCVSNALPPELGVTSW--DVVVNKLSNIWARFYIPKQ 84
           + E+  GL+++  NG V R++ +  ++  +P +   T    D + +K +N+  R Y P  
Sbjct: 10  VAEDCMGLLQLLSNGTVLRSESIDLITQQIPFKNNQTVLFKDSIYHKPNNLHLRLYKPIS 69

Query: 85  CQGK--LPLLVYFHGGGFCVGSAAWSCYHEFLAKVSAKAECIIMSVNYRLAPENPLPAPY 144
              +  LP++V+FHGGGFC GS +W  +H F   +++    +++S +YRLAPE+ LPA +
Sbjct: 70  ASNRTALPVVVFFHGGGFCFGSRSWPHFHNFCLTLASSLNALVVSPDYRLAPEHRLPAAF 129

Query: 145 EDGLKTISWLKQVALIGGKQNWW--SRYCDFTRIYLGGDSAGGNIAFNVAARLGGKTTAS 204
           ED    ++WL   A+  G  +W+      DF R+++ GDS+GGNIA  +A R G     S
Sbjct: 130 EDAEAVLTWLWDQAVSDGVNHWFEDGTDVDFDRVFVVGDSSGGNIAHQLAVRFG-----S 189

Query: 205 GAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPPRSALTMAVSDTYWRLALPSGANRD 264
           G++ L P+ +RG +L+ PFFGGE RT SE     P  + L++ + D +WRL+LP+GA RD
Sbjct: 190 GSIELTPVRVRGYVLMGPFFGGEERTNSEN---GPSEALLSLDLLDKFWRLSLPNGATRD 249

Query: 265 HPWCNPSAKGLFKVENLRVLPSLVCISELDILKDRNLEFCSALHR-EGKLINYVVHEGVG 324
           H   NP       +E++ + P LV +   ++L+DR  E+   L +  GK ++Y+  E   
Sbjct: 250 HHMANPFGPTSPTLESISLEPMLVIVGGSELLRDRAKEYAYKLKKMGGKRVDYIEFENKE 309

Query: 325 HAFQVLNKSQLSQTRTLEMIG 339
           H F     S  +  + L +IG
Sbjct: 310 HGFYSNYPSSEAAEQVLRIIG 322

BLAST of Carg21572 vs. TAIR 10
Match: AT5G62180.1 (carboxyesterase 20 )

HSP 1 Score: 164.5 bits (415), Expect = 1.6e-40
Identity = 96/267 (35.96%), Postives = 142/267 (53.18%), Query Frame = 0

Query: 62  SWDVVVNKLSNIWARFYIPKQC-------QGKLPLLVYFHGGGFCVGSAAWSCYHEFLAK 121
           S D+ VN+L + W R Y+P            KLP++VY+HGGGF + S     +H+F ++
Sbjct: 48  SKDLPVNQLKSTWLRLYLPSSAVNEGNVSSQKLPIVVYYHGGGFILCSVDMQLFHDFCSE 107

Query: 122 VSAKAECIIMSVNYRLAPENPLPAPYEDGLKTISWLKQVALIGGKQNWWSRYCDFTRIYL 181
           V+     I++S +YRLAPE+ LPA Y+DG++ + W+K          W   + DF+ ++L
Sbjct: 108 VARDLNAIVVSPSYRLAPEHRLPAAYDDGVEALDWIKT-----SDDEWIKSHADFSNVFL 167

Query: 182 GGDSAGGNIAFNVAARLGGKTTASGAVVLKPLVIRGNILIQPFFGGESRTKSEKFLVQPP 241
            G SAGGN+A+NV  R     +      L PL IRG IL  PFFGGE R++SE  L+   
Sbjct: 168 MGTSAGGNLAYNVGLR-----SVDSVSDLSPLQIRGLILHHPFFGGEERSESEIRLMND- 227

Query: 242 RSALTMAVSDTYWRLALPSGANRDHPWCNPS-AKGLFKVENLRVLPSLVCI--SELDILK 301
                  V+D  W L+LP G +RDH + NP+   G  K+E +  L   V +   E D + 
Sbjct: 228 -QVCPPIVTDVMWDLSLPVGVDRDHEYSNPTVGDGSEKLEKIGRLRWKVMMIGGEDDPMI 287

Query: 302 DRNLEFCSALHREGKLINYVVHEGVGH 319
           D   +    + ++G  +  V H   GH
Sbjct: 288 DLQKDVAKLMKKKG--VEVVEHYTGGH 300

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022944859.14.2e-205100.00probable carboxylesterase 6 [Cucurbita moschata] >KAG7013040.1 putative carboxyl... [more]
KAG6573982.19.3e-20599.71putative carboxylesterase 6, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022968602.14.6e-20499.42probable carboxylesterase 6 [Cucurbita maxima][more]
XP_023542364.14.8e-20198.55probable carboxylesterase 6 [Cucurbita pepo subsp. pepo][more]
XP_038893358.11.3e-19091.62probable carboxylesterase 6 [Benincasa hispida] >XP_038893359.1 probable carboxy... [more]
Match NameE-valueIdentityDescription
Q9SX251.8e-9752.11Probable carboxylesterase 6 OS=Arabidopsis thaliana OX=3702 GN=CXE6 PE=2 SV=1[more]
Q9LFR72.5e-8850.45Probable carboxylesterase 17 OS=Arabidopsis thaliana OX=3702 GN=CXE17 PE=1 SV=1[more]
Q9FG133.5e-5334.89Probable carboxylesterase 15 OS=Arabidopsis thaliana OX=3702 GN=CXE15 PE=2 SV=1[more]
Q0ZPV71.1e-4336.79Carboxylesterase 1 OS=Actinidia eriantha OX=165200 GN=CXE1 PE=1 SV=1[more]
Q5NUF43.0e-4134.042-hydroxyisoflavanone dehydratase OS=Glycyrrhiza echinata OX=46348 GN=HIDM PE=1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1FZC12.0e-205100.00probable carboxylesterase 6 OS=Cucurbita moschata OX=3662 GN=LOC111449259 PE=4 S... [more]
A0A6J1HTY62.2e-20499.42probable carboxylesterase 6 OS=Cucurbita maxima OX=3661 GN=LOC111467774 PE=4 SV=... [more]
A0A0A0KUG57.0e-19091.04Abhydrolase_3 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_5G6085... [more]
A0A1S3BFC81.7e-18889.88probable carboxylesterase 6 OS=Cucumis melo OX=3656 GN=LOC103489265 PE=4 SV=1[more]
A0A5A7SV761.7e-18889.88Putative carboxylesterase 6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaf... [more]
Match NameE-valueIdentityDescription
AT1G68620.11.2e-9852.11alpha/beta-Hydrolases superfamily protein [more]
AT5G16080.11.8e-8950.45carboxyesterase 17 [more]
AT5G06570.12.5e-5434.89alpha/beta-Hydrolases superfamily protein [more]
AT5G06570.22.5e-5434.89alpha/beta-Hydrolases superfamily protein [more]
AT5G62180.11.6e-4035.96carboxyesterase 20 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR029058Alpha/Beta hydrolase foldGENE3D3.40.50.1820alpha/beta hydrolasecoord: 32..345
e-value: 6.1E-69
score: 235.0
IPR029058Alpha/Beta hydrolase foldSUPERFAMILY53474alpha/beta-Hydrolasescoord: 24..344
IPR013094Alpha/beta hydrolase fold-3PFAMPF07859Abhydrolase_3coord: 90..321
e-value: 1.3E-53
score: 182.0
NoneNo IPR availablePANTHERPTHR23024:SF409CARBOXYLESTERASE 6-RELATEDcoord: 15..344
NoneNo IPR availablePANTHERPTHR23024ARYLACETAMIDE DEACETYLASEcoord: 15..344

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21572-RACarg21572-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016787 hydrolase activity