Carg21444 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21444
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RING/U-box superfamily protein
Location	Carg_Chr13: 6027023 .. 6028902 (+)
RNA-Seq Expression	Carg21444
Synteny	Carg21444

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGATCATCGTGAGATTTTTGGCCGTGGTTCTTCTCGTGTTCTAGCTGAGGAAGATGATGGTGAAGATTTTCGAAGTTGTTGCGAAGATCAAGAAGTATGGAAAGATAATGAATTTGCAGGGGAAGAACAGTCTGAGAAAGAGGAGGATCTTGATGAGTTTTCCGTGAAATTGTTCTTTAAAGGCGTTTCGATAGCGGGGATTGGTGATCCTGCTTCTGGTTTTTCTGGAATTGGGGTATTCATGGAGAAATCAGGAAAGGTCCCTTTTATTCAGGTCCAGAAGAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTTGAGGCTATGCAGAACGACATCCACTGTATTCGAGCTTTCACCGACTCCGATCCGTTATACAAACAGGTTATTACTCCCCGACCCGCCTAATCTTTTCTTGTTATGATTTATGCTAATATCTGTGGAAGTTCAAGAGAGTGGAGAGATGCATAAACTCTTTAGAGTGTTCTAGACATGTTCATACATCTTATATGCTTAAGATAATGTGAATGTTTGACAGATAACAAACGAAGAACAGCCTGAAATTCCACTTCTTGTAGCGCTGAAAGAAAGGATTCTTGAACATGCTAGCAATTTTGAAGCCTTTTCCCTAAACCTTTCTTCAAGTACCGATCTCGAGCAGCCATTTCATCTTGCAAAAGTGGCTATCGGGGTTCTTTCTCGTCCGAGAAAAGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGATAATAAACCATCTGCCATGATTGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTAATTGTTTGAAGACTTATGTTGATGGAAAAGTAGAGGCATCCCAAGTTCCCATTAGATGCCCTCAATTGAGATGCAAATACTACATTACAAACAATGAGTTTAGATCTTTTCTTCCCCTGACTTTATATGAATCATTAGAGAATACCTTAGCAGAAGCAAACCTTCGCTCGGATAGAATTTACTGCCCTTTTCCGAATTGCTCTGTTCTGTTAGATCCGAGCGAATGTTTGTCAGCTAGGGCGAGTTCATCAAGTCAATCAGATAATAGCTGTTTCGAGTGTCCTGTATGTCAGAGATTTATCTGTGTTGAATGTCAAGTTCCTTGGCATTCGTCGATGAGCTGCGAAGAATTTCAGAACGTCCCGTTAGAGGAGAGAGATGCTGCTGATATTACCTTACGCCATCTTGCACAAAACAAAAGGTGGCGCCGTTGTCAGGAGTGTCGTAGGATGATTGAATTGACTCAGGGTTGCTACCATATGACATGCTGGTAAGGCTTCTTCCTTCAAATCTTAATTTAGTGCATATTTTATAGCAGCCTTGTTGTTCAATGTGATGAACATGATTCGTGTATATATAACAAGTAGATCTAACTTATTAGATTCAGGGCTAGTTGGAGCAGGGCTGTATGTATATGACTTGCTTGATGTGTTTTACAGGTGTGGGCATGAATTCTGCTATTCATGTGGTGGTGAGTATCAAGATGGGCAACAGACGTGCCGATGCGCGTTGTGGGACGAAGAAAACTCCGAGGACATGGTCACCCATTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGATGCATACTCCGAGCAAGAGAGGTCACAATTAGCACTGATTCAACGATTCCTCGCCGGGGGATTTAGTTTGAGCGACCACCAACCGTATCAATCTCCACCACGATGTACAGAATCTTCTTATGTCGACACGATGAAGGACATACACCAGCTTCCTTGGCTTGAGAGGTTTGTTTCTCTGATAAGCGATAGCTATTACGAAGATTATGTACAATGA

mRNA sequence

ATGGATCATCGTGAGATTTTTGGCCGTGGTTCTTCTCGTGTTCTAGCTGAGGAAGATGATGGTGAAGATTTTCGAAGTTGTTGCGAAGATCAAGAAGTATGGAAAGATAATGAATTTGCAGGGGAAGAACAGTCTGAGAAAGAGGAGGATCTTGATGAGTTTTCCGTGAAATTGTTCTTTAAAGGCGTTTCGATAGCGGGGATTGGTGATCCTGCTTCTGGTTTTTCTGGAATTGGGGTATTCATGGAGAAATCAGGAAAGGTCCCTTTTATTCAGGTCCAGAAGAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTTGAGGCTATGCAGAACGACATCCACTGTATTCGAGCTTTCACCGACTCCGATCCGTTATACAAACAGATAACAAACGAAGAACAGCCTGAAATTCCACTTCTTGTAGCGCTGAAAGAAAGGATTCTTGAACATGCTAGCAATTTTGAAGCCTTTTCCCTAAACCTTTCTTCAAGTACCGATCTCGAGCAGCCATTTCATCTTGCAAAAGTGGCTATCGGGGTTCTTTCTCGTCCGAGAAAAGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGATAATAAACCATCTGCCATGATTGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTAATTGTTTGAAGACTTATGTTGATGGAAAAGTAGAGGCATCCCAAGTTCCCATTAGATGCCCTCAATTGAGATGCAAATACTACATTACAAACAATGAGTTTAGATCTTTTCTTCCCCTGACTTTATATGAATCATTAGAGAATACCTTAGCAGAAGCAAACCTTCGCTCGGATAGAATTTACTGCCCTTTTCCGAATTGCTCTGTTCTGTTAGATCCGAGCGAATGTTTGTCAGCTAGGGCGAGTTCATCAAGTCAATCAGATAATAGCTGTTTCGAGTGTCCTGTATGTCAGAGATTTATCTGTGTTGAATGTCAAGTTCCTTGGCATTCGTCGATGAGCTGCGAAGAATTTCAGAACGTCCCGTTAGAGGAGAGAGATGCTGCTGATATTACCTTACGCCATCTTGCACAAAACAAAAGGTGGCGCCGTTGTCAGGAGTGTCGTAGGATGATTGAATTGACTCAGGGTTGCTACCATATGACATGCTGGTGTGGGCATGAATTCTGCTATTCATGTGGTGGTGAGTATCAAGATGGGCAACAGACGTGCCGATGCGCGTTGTGGGACGAAGAAAACTCCGAGGACATGGTCACCCATTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGATGCATACTCCGAGCAAGAGAGGTCACAATTAGCACTGATTCAACGATTCCTCGCCGGGGGATTTAGTTTGAGCGACCACCAACCGTATCAATCTCCACCACGATGTACAGAATCTTCTTATGTCGACACGATGAAGGACATACACCAGCTTCCTTGGCTTGAGAGGTTTGTTTCTCTGATAAGCGATAGCTATTACGAAGATTATGTACAATGA

Coding sequence (CDS)

ATGGATCATCGTGAGATTTTTGGCCGTGGTTCTTCTCGTGTTCTAGCTGAGGAAGATGATGGTGAAGATTTTCGAAGTTGTTGCGAAGATCAAGAAGTATGGAAAGATAATGAATTTGCAGGGGAAGAACAGTCTGAGAAAGAGGAGGATCTTGATGAGTTTTCCGTGAAATTGTTCTTTAAAGGCGTTTCGATAGCGGGGATTGGTGATCCTGCTTCTGGTTTTTCTGGAATTGGGGTATTCATGGAGAAATCAGGAAAGGTCCCTTTTATTCAGGTCCAGAAGAAGCTTGATTTTTATGTTGATGAGTTAGTTGCTGATTATTTAGCTCTAATGGATGGTCTGGTTGAGGCTATGCAGAACGACATCCACTGTATTCGAGCTTTCACCGACTCCGATCCGTTATACAAACAGATAACAAACGAAGAACAGCCTGAAATTCCACTTCTTGTAGCGCTGAAAGAAAGGATTCTTGAACATGCTAGCAATTTTGAAGCCTTTTCCCTAAACCTTTCTTCAAGTACCGATCTCGAGCAGCCATTTCATCTTGCAAAAGTGGCTATCGGGGTTCTTTCTCGTCCGAGAAAAGGAGATAAATCAGTTGAAAATTGTTCTATTTGTTGTGATAATAAACCATCTGCCATGATTGTTGCATTGAAGTGTTGTCACAAGTTCTGCTCTAATTGTTTGAAGACTTATGTTGATGGAAAAGTAGAGGCATCCCAAGTTCCCATTAGATGCCCTCAATTGAGATGCAAATACTACATTACAAACAATGAGTTTAGATCTTTTCTTCCCCTGACTTTATATGAATCATTAGAGAATACCTTAGCAGAAGCAAACCTTCGCTCGGATAGAATTTACTGCCCTTTTCCGAATTGCTCTGTTCTGTTAGATCCGAGCGAATGTTTGTCAGCTAGGGCGAGTTCATCAAGTCAATCAGATAATAGCTGTTTCGAGTGTCCTGTATGTCAGAGATTTATCTGTGTTGAATGTCAAGTTCCTTGGCATTCGTCGATGAGCTGCGAAGAATTTCAGAACGTCCCGTTAGAGGAGAGAGATGCTGCTGATATTACCTTACGCCATCTTGCACAAAACAAAAGGTGGCGCCGTTGTCAGGAGTGTCGTAGGATGATTGAATTGACTCAGGGTTGCTACCATATGACATGCTGGTGTGGGCATGAATTCTGCTATTCATGTGGTGGTGAGTATCAAGATGGGCAACAGACGTGCCGATGCGCGTTGTGGGACGAAGAAAACTCCGAGGACATGGTCACCCATTCCATCCAAGAATCTGAGCAGTGGGCATGGGAGACTTTTAATTCCCTCCCCATGATAATGGATGCATACTCCGAGCAAGAGAGGTCACAATTAGCACTGATTCAACGATTCCTCGCCGGGGGATTTAGTTTGAGCGACCACCAACCGTATCAATCTCCACCACGATGTACAGAATCTTCTTATGTCGACACGATGAAGGACATACACCAGCTTCCTTGGCTTGAGAGGTTTGTTTCTCTGATAAGCGATAGCTATTACGAAGATTATGTACAATGA

Protein sequence

MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFFKGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEENSEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSPPRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ

Homology

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BLAST of Carg21444 vs. NCBI nr
Match: KAG7019427.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1075.8 bits (2781), Expect = 0.0e+00
Identity = 518/518 (100.00%), Postives = 518/518 (100.00%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

[Close]

BLAST of Carg21444 vs. NCBI nr
Match: XP_022927282.1 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1056.6 bits (2731), Expect = 6.9e-305
Identity = 506/518 (97.68%), Postives = 512/518 (98.84%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAE+DDGEDFRSCCED EVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRC LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+SSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

[Close]

BLAST of Carg21444 vs. NCBI nr
Match: XP_023519477.1 (E3 ubiquitin-protein ligase arih1-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053.5 bits (2723), Expect = 5.9e-304
Identity = 506/518 (97.68%), Postives = 512/518 (98.84%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDH EIFGRGSSRVLAEEDDGEDFRSCCED EVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHPEIFGRGSSRVLAEEDDGEDFRSCCEDHEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGI VFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGICVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHCIRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLLALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYITN EFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNTEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
            HLAQNKRWRRC+ECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN
Sbjct: 361 HHLAQNKRWRRCEECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           P+CT+SSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PQCTDSSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

[Close]

BLAST of Carg21444 vs. NCBI nr
Match: KAG6583802.1 (putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1046.6 bits (2705), Expect = 7.2e-302
Identity = 503/503 (100.00%), Postives = 503/503 (100.00%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLER 504
           PRCTESSYVDTMKDIHQLPWLER
Sbjct: 481 PRCTESSYVDTMKDIHQLPWLER 503

[Close]

BLAST of Carg21444 vs. NCBI nr
Match: XP_023000867.1 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 1034.6 bits (2674), Expect = 2.8e-298
Identity = 497/518 (95.95%), Postives = 506/518 (97.68%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREI GRGSSRVLAEEDDGEDFRSCCEDQEVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREISGRGSSRVLAEEDDGEDFRSCCEDQEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFME SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMENSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHCIRAFTDSDPLYKQITNEE+PEIPLLVALKERILEHASNFEAFSLNLSS+T+LEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEEPEIPLLVALKERILEHASNFEAFSLNLSSNTELEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVE+
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVES 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
            QVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEAN R DRIYCPFPNCSVLLDP
Sbjct: 241 FQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANFRLDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQ VPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQKVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCG+EFCYSCG EYQDGQQTC CALWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGYEFCYSCGAEYQDGQQTCECALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE +VTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEYLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+SS VDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTDSSDVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

[Close]

BLAST of Carg21444 vs. ExPASy Swiss-Prot
Match: D3YYI7 (Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 PE=3 SV=2)

HSP 1 Score: 104.0 bits (258), Expect = 5.2e-21
Identity = 66/209 (31.58%), Postives = 95/209 (45.45%), Query Frame = 0


Query: 204 CSICCDNKPSAMIVALKCCHK-FCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEFR 263
           C +C ++KP   I  L CC K  C  CLK Y+  +V+  QV I+CP   C  ++   E  
Sbjct: 236 CRVCLEDKP---IKPLPCCKKAVCEECLKIYLSSQVQLGQVEIKCPVTECFEFL--EETT 295

Query: 264 SFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECP 323
               LT  +S++        R D    P P C       +       S S+S     +CP
Sbjct: 296 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESRYK-IQCP 355

Query: 324 VCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQ-----NKRWRRCQECRR 383
            CQ   C +C  PWH  ++C+E++          D  LRH A       +  ++C +C+ 
Sbjct: 356 TCQLIWCFKCHSPWHEGVNCKEYKK--------GDKLLRHWASEIEHGQRNAQKCPKCKI 415

Query: 384 MIELTQGCYHMTC-WCGHEFCYSCGGEYQ 406
            I+ T+GC HMTC  C   FCY CG  Y+
Sbjct: 416 HIQRTEGCDHMTCSQCNTNFCYRCGERYR 430

[Close]

BLAST of Carg21444 vs. ExPASy Swiss-Prot
Match: Q8TC41 (Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE=2 SV=4)

HSP 1 Score: 103.6 bits (257), Expect = 6.8e-21
Identity = 66/209 (31.58%), Postives = 95/209 (45.45%), Query Frame = 0


Query: 204 CSICCDNKPSAMIVALKCCHK-FCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEFR 263
           C +C ++KP   I  L CC K  C  CLK Y+  +V+  QV I+CP   C  ++   E  
Sbjct: 263 CRVCLEDKP---IKPLPCCKKAVCEECLKVYLSAQVQLGQVEIKCPITECFEFL--EETT 322

Query: 264 SFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECP 323
               LT  +S++        R D    P P C       +       S S+S     +CP
Sbjct: 323 VVYNLTHEDSIKYKYFLELGRIDSSTKPCPQCKHFTTFKKKGHIPTPSRSESKYK-IQCP 382

Query: 324 VCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQ-----NKRWRRCQECRR 383
            CQ   C +C  PWH  ++C+E++          D  LRH A       +  ++C +C+ 
Sbjct: 383 TCQFVWCFKCHSPWHEGVNCKEYKK--------GDKLLRHWASEIEHGQRNAQKCPKCKI 442

Query: 384 MIELTQGCYHMTC-WCGHEFCYSCGGEYQ 406
            I+ T+GC HMTC  C   FCY CG  Y+
Sbjct: 443 HIQRTEGCDHMTCSQCNTNFCYRCGERYR 457

[Close]

BLAST of Carg21444 vs. ExPASy Swiss-Prot
Match: Q4KLT0 (Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 PE=2 SV=1)

HSP 1 Score: 96.3 bits (238), Expect = 1.1e-18
Identity = 61/211 (28.91%), Postives = 91/211 (43.13%), Query Frame = 0


Query: 203 NCSICCDNKPSAMIVALKCCHK-FCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEF 262
           +C +C +++    I  L CC K  C  CLK Y+  +V+  Q  I+CP   C  ++  +  
Sbjct: 2   SCRVCLEDR---SIKPLPCCKKPVCDECLKRYLSSQVQLGQAEIQCPITECNKHLDESTI 61

Query: 263 RSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSC-FE 322
              LP       +  L  +  R D    P P C               + ++S+N    +
Sbjct: 62  LYSLPHDDIIKYKYFLELS--RMDSSTKPCPQCKHFTTFKR--KTHIPNPTKSENKLKIQ 121

Query: 323 CPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQ-----NKRWRRCQEC 382
           CP CQ   C  C  PWH  ++C E++          D  LRH A       +  ++C  C
Sbjct: 122 CPSCQFIWCFRCHAPWHEGVNCREYKK--------GDKLLRHWANEIEHGQRNAQKCPRC 181

Query: 383 RRMIELTQGCYHMTC-WCGHEFCYSCGGEYQ 406
           +  I+ T+GC HMTC  C   FCY CG  Y+
Sbjct: 182 KVHIQRTEGCDHMTCSQCNTNFCYRCGERYR 197

[Close]

BLAST of Carg21444 vs. ExPASy Swiss-Prot
Match: Q9SKC3 (Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI9 PE=2 SV=1)

HSP 1 Score: 89.4 bits (220), Expect = 1.3e-16
Identity = 64/263 (24.33%), Postives = 103/263 (39.16%), Query Frame = 0


Query: 187 AIGVLSRP----------RKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDG 246
           A+G+L  P          +K  K    C IC ++     I  + C H +C  C   Y+  
Sbjct: 101 AVGLLKEPVVDFNGGEKDKKCRKVNIQCGICFESYTREEIARVSCGHPYCKTCWAGYITT 160

Query: 247 KVEASQ--VPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDR--IYCPFP 306
           K+E     + ++CP+  C   +  +         + E     +  + +   +   +CP P
Sbjct: 161 KIEDGPGCLRVKCPEPSCSAAVGKDMIEDVTETKVNEKYSRYILRSYVEDGKKIKWCPSP 220

Query: 307 NCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEE 366
            C   ++         S SS  D SC    +C    C  C    HS + C+       + 
Sbjct: 221 GCGYAVE------FGGSESSSYDVSC----LCSYRFCWNCSEDAHSPVDCDTVSKWIFKN 280

Query: 367 RDAADITLRHLAQNKRW-----RRCQECRRMIELTQGCYHMTCW--CGHEFCYSCGGEYQ 426
           +D         ++NK W     + C EC+R IE   GC HMTC   CGHEFC+ C   Y+
Sbjct: 281 QDE--------SENKNWMLANSKPCPECKRPIEKNDGCNHMTCSAPCGHEFCWICLKAYR 340

Query: 427 DGQQTCRCALWDEENSEDMVTHS 429
                C   + ++  S+  +  S
Sbjct: 341 RHSGACNRFVVEQAESKRALLQS 345

[Close]

BLAST of Carg21444 vs. ExPASy Swiss-Prot
Match: Q6T486 (Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 GN=rbrA PE=3 SV=1)

HSP 1 Score: 86.3 bits (212), Expect = 1.1e-15
Identity = 68/282 (24.11%), Postives = 115/282 (40.78%), Query Frame = 0


Query: 203 NCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQ--VPIRCPQLRCKYYITNNE 262
           +C IC ++ P     AL C H++C  C K Y++ KV      +   CP  +CK  +  + 
Sbjct: 138 SCLICLEDYPPTQTFALICNHRYCLPCYKNYLEIKVSEGPECIYTPCPAPKCKVIVHQDA 197

Query: 263 FRSFLPLTLYESLENTLAEANLRSDR--IYCPFPNCSVLLDPSECLSARASSSSQSDNSC 322
           F+  +   ++E   N + ++ +  +    +CP P C          S R     + +   
Sbjct: 198 FKQIVSPEVFERFNNFILKSYVDDNPQVKWCPAPGC--------IYSIRCDRKERKEAVN 257

Query: 323 FECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQNKRWRRCQECRRM 382
            +C     F C + ++  H    C +      +  D ++     LA  K   +C ECR  
Sbjct: 258 CKCGFQYCFNCNDYEIGDHMPCPCSQVDKWLQKASDESENVTWMLANTK---KCPECRSP 317

Query: 383 IELTQGCYHMTC-----WCGHEFCYSCGGEYQDGQQTC----RCALWDEENS--EDMVTH 442
           IE   GC HMTC      CG EFC+ C G + +   T      C  +D+  +  +D   H
Sbjct: 318 IEKNGGCMHMTCRKNAGGCGFEFCWLCRGPWTEHGSTTGGYYNCNKYDKSKAKEDDDKAH 377

Query: 443 SIQ---ESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA 467
             +   E+  + +  + S    M    EQ R+     Q+ L+
Sbjct: 378 DAKTELEAYMFYYHRYESHRNAMKIADEQRRNAHLKEQQILS 408

[Close]

BLAST of Carg21444 vs. ExPASy TrEMBL
Match: A0A6J1EGQ6 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111434167 PE=3 SV=1)

HSP 1 Score: 1056.6 bits (2731), Expect = 3.4e-305
Identity = 506/518 (97.68%), Postives = 512/518 (98.84%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAE+DDGEDFRSCCED EVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRC LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+SSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

[Close]

BLAST of Carg21444 vs. ExPASy TrEMBL
Match: A0A6J1KEV2 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495184 PE=3 SV=1)

HSP 1 Score: 1034.6 bits (2674), Expect = 1.4e-298
Identity = 497/518 (95.95%), Postives = 506/518 (97.68%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREI GRGSSRVLAEEDDGEDFRSCCEDQEVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREISGRGSSRVLAEEDDGEDFRSCCEDQEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFME SGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMENSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHCIRAFTDSDPLYKQITNEE+PEIPLLVALKERILEHASNFEAFSLNLSS+T+LEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEEPEIPLLVALKERILEHASNFEAFSLNLSSNTELEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVE+
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVES 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
            QVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEAN R DRIYCPFPNCSVLLDP
Sbjct: 241 FQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANFRLDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQ VPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQKVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCG+EFCYSCG EYQDGQQTC CALWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGYEFCYSCGAEYQDGQQTCECALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE +VTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEYLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+SS VDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTDSSDVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 518

[Close]

BLAST of Carg21444 vs. ExPASy TrEMBL
Match: A0A6J1EKK2 (probable E3 ubiquitin-protein ligase rbrA isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111434167 PE=3 SV=1)

HSP 1 Score: 988.4 bits (2554), Expect = 1.1e-284
Identity = 478/518 (92.28%), Postives = 484/518 (93.44%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREIFGRGSSRVLAE+DDGEDFRSCCED EVWKDNEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREIFGRGSSRVLAEDDDGEDFRSCCEDHEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFMEKSGKVPFI                            Q
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMEKSGKVPFI----------------------------Q 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIH IRAFTDSDPLYKQITNEEQPEIPLL+ALKERILEHASNFEAFSLNLSSSTDLEQP
Sbjct: 121 NDIHSIRAFTDSDPLYKQITNEEQPEIPLLIALKERILEHASNFEAFSLNLSSSTDLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRC+YYITNNEFRSFLPLTLYESL+NTLAEANLRSDRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCQYYITNNEFRSFLPLTLYESLDNTLAEANLRSDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRC LWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCTLWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SEDMVTHSIQESEQWAWETFNSLPMIMD YSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDTYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+SSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTDSSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 490

[Close]

BLAST of Carg21444 vs. ExPASy TrEMBL
Match: A0A6J1KNW4 (ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111495184 PE=3 SV=1)

HSP 1 Score: 966.5 bits (2497), Expect = 4.6e-278
Identity = 469/518 (90.54%), Postives = 478/518 (92.28%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           MDHREI GRGSSRVLAEEDDGEDFRSCCEDQEVWK+NEFAGEEQSEKEEDLDEFSVKLFF
Sbjct: 1   MDHREISGRGSSRVLAEEDDGEDFRSCCEDQEVWKENEFAGEEQSEKEEDLDEFSVKLFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIAGIGDPASG SGIGVFME SGKVPFI                            Q
Sbjct: 61  KGVSIAGIGDPASGLSGIGVFMENSGKVPFI----------------------------Q 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           NDIHCIRAFTDSDPLYKQITNEE+PEIPLLVALKERILEHASNFEAFSLNLSS+T+LEQP
Sbjct: 121 NDIHCIRAFTDSDPLYKQITNEEEPEIPLLVALKERILEHASNFEAFSLNLSSNTELEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLSRP+KGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVE+
Sbjct: 181 FHLAKVAIGVLSRPKKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVES 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
            QVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEAN R DRIYCPFPNCSVLLDP
Sbjct: 241 FQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANFRLDRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQ VPLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQKVPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
           RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCG+EFCYSCG EYQDGQQTC CALWDEEN
Sbjct: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGYEFCYSCGAEYQDGQQTCECALWDEEN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE +VTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SEYLVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+SS VDTMKDIHQLPWLERFVSLISDSYYEDYVQ
Sbjct: 481 PRCTDSSDVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 490

[Close]

BLAST of Carg21444 vs. ExPASy TrEMBL
Match: A0A1S3C9E4 (E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 462/518 (89.19%), Postives = 490/518 (94.59%), Query Frame = 0


Query: 1   MDHREIFGRGSSRVLAEEDDGEDFRSCCEDQEVWKDNEFAGEEQSEKEEDLDEFSVKLFF 60
           M+HR+IFGRGSS   AEEDD E+FRSCCED EVWK+ E A EE+S  EEDLDEFSVK+FF
Sbjct: 1   MEHRQIFGRGSSHGPAEEDDVEEFRSCCEDHEVWKETEDAVEEES--EEDLDEFSVKMFF 60

Query: 61  KGVSIAGIGDPASGFSGIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQ 120
           KGVSIA IGDPASG SGIGVFMEKSGK+PF+QVQKKLDFYVDELVADYLALMDGLVEAMQ
Sbjct: 61  KGVSIAEIGDPASGLSGIGVFMEKSGKIPFVQVQKKLDFYVDELVADYLALMDGLVEAMQ 120

Query: 121 NDIHCIRAFTDSDPLYKQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQP 180
           ND+ CIRAFTDSD LYKQITNEE+PEIPLLVALKERILEHASNFE+FSL LSSS DLEQP
Sbjct: 121 NDVRCIRAFTDSDLLYKQITNEEEPEIPLLVALKERILEHASNFESFSLILSSSADLEQP 180

Query: 181 FHLAKVAIGVLSRPRKGDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEA 240
           FHLAKVAIGVLS P+KGDKSVENCSICCDNKPSAM+VALKCCHKFCS CLKTYVDGKV+A
Sbjct: 181 FHLAKVAIGVLSCPQKGDKSVENCSICCDNKPSAMMVALKCCHKFCSQCLKTYVDGKVDA 240

Query: 241 SQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLRSDRIYCPFPNCSVLLDP 300
           SQVPIRCPQLRCKYYIT+NEF+SFLPLTLYE LENTLAEAN+ +DRIYCPFPNCSVLLDP
Sbjct: 241 SQVPIRCPQLRCKYYITSNEFKSFLPLTLYELLENTLAEANIHADRIYCPFPNCSVLLDP 300

Query: 301 SECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITL 360
           SECLSARASSSSQSDNSC ECPVCQRFICVECQVPWHSS+SCEEFQN+PLEERD ADITL
Sbjct: 301 SECLSARASSSSQSDNSCIECPVCQRFICVECQVPWHSSVSCEEFQNIPLEERDTADITL 360

Query: 361 RHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEEN 420
             LAQNKRWRRCQEC RMIELTQGC+HMTCWCGHEFCYSCG EYQDGQQTC+CA WDE+N
Sbjct: 361 HRLAQNKRWRRCQECHRMIELTQGCFHMTCWCGHEFCYSCGVEYQDGQQTCQCAFWDEDN 420

Query: 421 SEDMVTHSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480
           SE++VTHS+QESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP
Sbjct: 421 SENLVTHSVQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLAGGFSLSDHQPYQSP 480

Query: 481 PRCTESSYVDTMKDIHQLPWLERFVSLISDSYYEDYVQ 519
           PRCT+ SYVDTMKDIHQLPWLERFVS+ISDSYYEDYVQ
Sbjct: 481 PRCTD-SYVDTMKDIHQLPWLERFVSVISDSYYEDYVQ 515

[Close]

BLAST of Carg21444 vs. TAIR 10
Match: AT4G19670.1 (RING/U-box superfamily protein )

HSP 1 Score: 642.5 bits (1656), Expect = 2.9e-184
Identity = 322/514 (62.65%), Postives = 402/514 (78.21%), Query Frame = 0


Query: 20  DGEDFRSCCEDQEVW--KDNEFAGEEQSEKEEDL-DEFSVKLFFKGVSIAGIGDPASGFS 79
           D E+FRSCC D+E+W    ++ A   + EK++DL DEFSVK+ FKGVSI   GD  SG+S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIHCIRAFTDSDPLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A TDS+ LY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 KQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPRK 199
            QIT EEQ EIPLLVAL+ER+LE  SN   F L L+   DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCKYYI 259
           GDK +ENCSICC+++ S M+++LKC HKFCS+C+KTYV+GKV+ S+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 TNNEFRSFLPLTLYESLENTLAEANL---RSDRIYCPFPNCSVLLDPSECLS---ARASS 319
           +  E +SFLP+T ++S E    EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQNKRW 379
           S+QS+NS C ECPVC+RF+CV+C VPWH+SMSCEEFQ +P++ER   DITL  LA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEENSED---MVT 439
           RRCQ+CR MIEL QGC HMTC CGHEFCYSCG EY++GQQTC CA WD++  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TESSYVD-TMKDIHQLPWLERFVSLISDSYYEDY 517
           TESSYV+  MKD+HQLPWLERFVS+ISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

[Close]

BLAST of Carg21444 vs. TAIR 10
Match: AT4G19670.2 (RING/U-box superfamily protein )

HSP 1 Score: 642.5 bits (1656), Expect = 2.9e-184
Identity = 322/514 (62.65%), Postives = 402/514 (78.21%), Query Frame = 0


Query: 20  DGEDFRSCCEDQEVW--KDNEFAGEEQSEKEEDL-DEFSVKLFFKGVSIAGIGDPASGFS 79
           D E+FRSCC D+E+W    ++ A   + EK++DL DEFSVK+ FKGVSI   GD  SG+S
Sbjct: 20  DEEEFRSCCGDEELWLKGSDDIAKVVEEEKKDDLDDEFSVKMVFKGVSIFERGDLGSGYS 79

Query: 80  GIGVFMEKSGKVPFIQVQKKLDFYVDELVADYLALMDGLVEAMQNDIHCIRAFTDSDPLY 139
           GIGV +E+SG +  IQVQKKLDFYV+E VA+YLALMDGL  A+QN++  + A TDS+ LY
Sbjct: 80  GIGVVLERSGDLELIQVQKKLDFYVEESVANYLALMDGLEVALQNNLSSVVAVTDSELLY 139

Query: 140 KQITNEEQPEIPLLVALKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPRK 199
            QIT EEQ EIPLLVAL+ER+LE  SN   F L L+   DL++   LA+VA+G++S    
Sbjct: 140 NQITREEQLEIPLLVALRERVLEKTSNLNGFVLKLAPFCDLDEALSLAQVAVGIVSSNLD 199

Query: 200 GDKSVENCSICCDNKPSAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCKYYI 259
           GDK +ENCSICC+++ S M+++LKC HKFCS+C+KTYV+GKV+ S+VPIRCPQ++CK+Y+
Sbjct: 200 GDKPIENCSICCEDRQSEMMLSLKCTHKFCSHCMKTYVEGKVKTSEVPIRCPQVQCKHYL 259

Query: 260 TNNEFRSFLPLTLYESLENTLAEANL---RSDRIYCPFPNCSVLLDPSECLS---ARASS 319
           +  E +SFLP+T ++S E    EAN+    + +IYCP+PNCS LLDP ECLS   A +SS
Sbjct: 260 SAAECKSFLPVTTFKSFE----EANVCSKNNGKIYCPYPNCSFLLDPQECLSSGRASSSS 319

Query: 320 SSQSDNS-CFECPVCQRFICVECQVPWHSSMSCEEFQNVPLEERDAADITLRHLAQNKRW 379
           S+QS+NS C ECPVC+RF+CV+C VPWH+SMSCEEFQ +P++ER   DITL  LA+ KRW
Sbjct: 320 STQSENSCCVECPVCERFVCVDCGVPWHASMSCEEFQILPVDERYPDDITLHRLARYKRW 379

Query: 380 RRCQECRRMIELTQGCYHMTCWCGHEFCYSCGGEYQDGQQTCRCALWDEENSED---MVT 439
           RRCQ+CR MIEL QGC HMTC CGHEFCYSCG EY++GQQTC CA WD++  E    +  
Sbjct: 380 RRCQQCRIMIELAQGCNHMTCRCGHEFCYSCGAEYREGQQTCTCAFWDDDEEEQENAVEE 439

Query: 440 HSIQESEQWAWETFNSLPMIMDAYSEQERSQLALIQRFLA-GGFSLSDHQ-PYQS-PPRC 499
           ++IQE EQW W+TFNS+P +MDAYSEQERSQLALIQRFLA GGFSLSDH   YQS PP C
Sbjct: 440 NTIQEVEQWPWDTFNSIPTVMDAYSEQERSQLALIQRFLAGGGFSLSDHHTTYQSPPPPC 499

Query: 500 TESSYVD-TMKDIHQLPWLERFVSLISDSYYEDY 517
           TESSYV+  MKD+HQLPWLERFVS+ISD YYE+Y
Sbjct: 500 TESSYVEAAMKDLHQLPWLERFVSVISDDYYEEY 529

[Close]

BLAST of Carg21444 vs. TAIR 10
Match: AT5G60250.1 (zinc finger (C3HC4-type RING finger) family protein )

HSP 1 Score: 197.6 bits (501), Expect = 2.5e-50
Identity = 125/379 (32.98%), Postives = 200/379 (52.77%), Query Frame = 0


Query: 57  KLFFKGV--SIAGIGDPASGFSGIGV-FMEKSGKVPFIQVQKKLDFYVDELVADYLALMD 116
           +L+FKG+     G G      SG GV   ++   + F      +D  +    A+  AL+ 
Sbjct: 146 RLYFKGLVSDENGKGKMTDVVSGFGVAICDQRDNLLFEMKGPLIDNGMSRQGAELKALIR 205

Query: 117 GLVEAMQNDIHCIRAFTDSDPLYKQITNE---EQPEIPLLVALKERILEHASNFEAFSLN 176
           GL EA++  I  I  F DS P+++ +T +   +Q +I LL+   + I++H S+++     
Sbjct: 206 GLTEALKLGIKHIVFFCDSYPIFQYVTGKWMAKQKKISLLLDDLQSIMQHFSSYQHV--- 265

Query: 177 LSSSTDLEQPFHLAKVAIGVLSRPRKGDKSV-----ENCSICC-DNKPSAMIVALKCCHK 236
           L +  D++  + LA+ +I  +  P +  +       E C+IC  D     M    KC H+
Sbjct: 266 LVARNDVKFAYKLARESILSMVTPHEDPRQAKAVLKEECAICFNDIVAEGMFSVDKCRHR 325

Query: 237 FCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANLR- 296
           FC  C+K +V+ K+     P +CP   CK  +  +     L   L +  +  L E  +  
Sbjct: 326 FCFQCVKQHVEVKLLHGMAP-KCPHDGCKSELVIDACGKLLTPKLSKLWQQRLQENAIPV 385

Query: 297 SDRIYCPFPNCSVLLDPSECL-SARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSC 356
           ++R+YCP+P CS L+  ++   SA++  S    +    C  C+   CV+C+VPWH ++SC
Sbjct: 386 TERVYCPYPRCSALMSKTKISESAKSLLSLYPKSGVRRCVECRGLFCVDCKVPWHGNLSC 445

Query: 357 EEFQNVPLEERDAADITLRHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSCGG 416
            E++ +   E  A D+ L+ LA NK WR+C +C+ MIEL+QGC H+TC CGHEFCY+CGG
Sbjct: 446 TEYKKLH-PEPPADDVKLKSLANNKMWRQCGKCQHMIELSQGCNHITCRCGHEFCYNCGG 505

Query: 417 EYQDGQQTC--RCALWDEE 420
            +     TC  RC  W+EE
Sbjct: 506 GWNKIMGTCLNRCPTWNEE 519

[Close]

BLAST of Carg21444 vs. TAIR 10
Match: AT2G25360.1 (RING/U-box superfamily protein )

HSP 1 Score: 168.3 bits (425), Expect = 1.6e-41
Identity = 106/311 (34.08%), Postives = 168/311 (54.02%), Query Frame = 0


Query: 109 LALMDGLVEAMQNDIHCIRAFTDSDPLYKQIT--NEEQPEIPLLVALKERILEHASNFEA 168
           +AL+ GL E+    I  +  + D   LY+ I    + + ++  LV   + +LE  +  +A
Sbjct: 1   MALIRGLRESFDLGIRQVVVYCDDQLLYQYIVGRGKIKKKVVHLVDEFQLLLEEMTYTDA 60

Query: 169 FSLNLSSSTDLEQPFHLAKVAIGVLSRPRKGDKSVENCSICCDNKPS--AMIVALKCCHK 228
              +L +  D+   F LA+ AI       + D   E CSIC +            KC H+
Sbjct: 61  ---DLIALNDVNFAFKLAREAI-----VSRDDVKAEICSICFEETEGERMFFTTEKCVHR 120

Query: 229 FCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEFRSFLPLTLYESLENTLAEANL-R 288
            C  C+K YV+ K+ +  VP  C    CK+ +T       L L L E  +  + E ++  
Sbjct: 121 HCFPCVKQYVEVKLLSGTVP-TCLDDGCKFKLTLESCSKVLTLELIEMWKQKMKEDSIPA 180

Query: 289 SDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICVECQVPWHSSMSCE 348
           ++RIYCP+PNCS+L+  +E  S    S   +D SC +C  C  F C++C+VP HS +SC 
Sbjct: 181 AERIYCPYPNCSMLMSKTELSS---ESDLSNDRSCVKC--CGLF-CIDCKVPSHSDLSCA 240

Query: 349 EFQNV---PLEERDAADITLRHLAQNKRWRRCQECRRMIELTQGCYHMTCWCGHEFCYSC 408
           E++ +   PL +    ++ L+ LA++K+WR+C+ CR MIEL+  C HMTC CG++FCY C
Sbjct: 241 EYKKLHHDPLVD----ELKLKSLAKDKKWRQCKMCRHMIELSHACNHMTCRCGYQFCYQC 292

Query: 409 GGEYQDGQQTC 412
             E+++ Q+TC
Sbjct: 301 EVEWKNDQKTC 292

[Close]

BLAST of Carg21444 vs. TAIR 10
Match: AT2G25370.1 (RING/U-box superfamily protein )

HSP 1 Score: 167.2 bits (422), Expect = 3.6e-41
Identity = 122/378 (32.28%), Postives = 197/378 (52.12%), Query Frame = 0


Query: 41  GEEQSEKEEDLDEFSVKLFFKG-VSIAGIGDPA-----SGFSGIGVFMEKSGKVPFIQVQ 100
           GE  S   + +D    +L FKG V+   + D       +GF G+ +  EK   +  I+ +
Sbjct: 139 GEGSSPAAKFMDNVVYRLCFKGLVNYEAVADDVEKAVKAGF-GVAICDEKDNLLHEIK-E 198

Query: 101 KKLDFYVDELVADYLALMDGLVEAMQNDIHCIRAFTDSDPLYKQI--TNEEQPEIPLLVA 160
              D  +     + +AL+ GL E+    +  +  + D D +Y+ I    + + +I  LV 
Sbjct: 199 SLRDIEISRRGVEIMALVRGLSESFDLGMRNVVIYCDDDWIYQSIIGRGKSKKKIDHLVE 258

Query: 161 LKERILEHASNFEAFSLNLSSSTDLEQPFHLAKVAIGVLSRPRKGDKSVENCSICCDNKP 220
             + ILE  +  +A    L +  D++  F LA+ AIG  S     ++  E C I  +   
Sbjct: 259 EVQGILEKMACIDAV---LVARNDVKFAFRLAREAIGRNSVDVNAEQG-ETCGIFFEETD 318

Query: 221 -SAMIVALKCCHKFCSNCLKTYVDGKVEASQVPIRCPQLRCKYYITNNEFRSFLPLTLYE 280
              M V  KC H+ C  C+K +V  K+ +   P  C +  CK+ +T       L L L E
Sbjct: 319 VEHMFVTEKCLHRHCFPCVKQHVKVKLRSGTEP-TCLEYGCKFKLTLERCSKVLTLKLIE 378

Query: 281 SLENTLAEANL-RSDRIYCPFPNCSVLLDPSECLSARASSSSQSDNSCFECPVCQRFICV 340
             +  + E ++  ++RIYCP+PNCS+L+  +E LS+ A  S+    +C +C  C  F C+
Sbjct: 379 MWKQKMKEDSIPAAERIYCPYPNCSMLMSKTE-LSSEADLSNV--RTCVKC--CGLF-CI 438

Query: 341 ECQVPWHSSMSCEEFQNV---PLEERDAADITLRHLAQNKRWRRCQECRRMIELTQGCYH 400
           +C+VP H+ +S ++++ +   PL +    D+ L+ LA +K WR+C +CR MIEL+ GC H
Sbjct: 439 DCKVPSHTDLSYDDYKKLHPDPLVD----DLKLKSLANDKMWRQCVKCRHMIELSHGCNH 498

Query: 401 MTCWCGHEFCYSCGGEYQ 406
           MTC CG+EFCY CG E+Q
Sbjct: 499 MTCRCGYEFCYECGIEWQ 499

The following BLAST results are available for this feature:

BLAST of Carg21444 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7019427.1	0.0e+00	100.00	putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. arg...	[more]
XP_022927282.1	6.9e-305	97.68	ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita mosc...	[more]
XP_023519477.1	5.9e-304	97.68	E3 ubiquitin-protein ligase arih1-like isoform X1 [Cucurbita pepo subsp. pepo]	[more]
KAG6583802.1	7.2e-302	100.00	putative E3 ubiquitin-protein ligase, partial [Cucurbita argyrosperma subsp. sor...	[more]
XP_023000867.1	2.8e-298	95.95	ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 [Cucurbita maxi...	[more]

BLAST of Carg21444 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
D3YYI7	5.2e-21	31.58	Probable E3 ubiquitin-protein ligase RNF217 OS=Mus musculus OX=10090 GN=Rnf217 P...	[more]
Q8TC41	6.8e-21	31.58	Probable E3 ubiquitin-protein ligase RNF217 OS=Homo sapiens OX=9606 GN=RNF217 PE...	[more]
Q4KLT0	1.1e-18	28.91	Probable E3 ubiquitin-protein ligase RNF217 OS=Xenopus laevis OX=8355 GN=rnf217 ...	[more]
Q9SKC3	1.3e-16	24.33	Probable E3 ubiquitin-protein ligase ARI9 OS=Arabidopsis thaliana OX=3702 GN=ARI...	[more]
Q6T486	1.1e-15	24.11	Probable E3 ubiquitin-protein ligase rbrA OS=Dictyostelium discoideum OX=44689 G...	[more]

BLAST of Carg21444 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EGQ6	3.4e-305	97.68	ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita mo...	[more]
A0A6J1KEV2	1.4e-298	95.95	ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X1 OS=Cucurbita ma...	[more]
A0A6J1EKK2	1.1e-284	92.28	probable E3 ubiquitin-protein ligase rbrA isoform X2 OS=Cucurbita moschata OX=36...	[more]
A0A6J1KNW4	4.6e-278	90.54	ATP-dependent RNA helicase DEAH12, chloroplastic-like isoform X2 OS=Cucurbita ma...	[more]
A0A1S3C9E4	2.3e-277	89.19	E3 ubiquitin-protein ligase arih1 OS=Cucumis melo OX=3656 GN=LOC103498311 PE=3 S...	[more]

BLAST of Carg21444 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G19670.1	2.9e-184	62.65	RING/U-box superfamily protein	[more]
AT4G19670.2	2.9e-184	62.65	RING/U-box superfamily protein	[more]
AT5G60250.1	2.5e-50	32.98	zinc finger (C3HC4-type RING finger) family protein	[more]
AT2G25360.1	1.6e-41	34.08	RING/U-box superfamily protein	[more]
AT2G25370.1	3.6e-41	32.28	RING/U-box superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001841	Zinc finger, RING-type	SMART	SM00184	ring_2	coord: 204..250 e-value: 0.0012 score: 28.1
IPR001841	Zinc finger, RING-type	PROSITE	PS50089	ZF_RING_2	coord: 204..251 score: 10.836942
IPR002867	IBR domain	SMART	SM00647	ibrneu5	coord: 267..342 e-value: 3.3E-8 score: 43.3 coord: 352..411 e-value: 0.086 score: 13.7
IPR002867	IBR domain	PFAM	PF01485	IBR	coord: 280..342 e-value: 1.7E-8 score: 34.5 coord: 362..411 e-value: 2.8E-6 score: 27.4
None	No IPR available	GENE3D	1.20.120.1750		coord: 340..465 e-value: 1.6E-15 score: 59.7
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..24
None	No IPR available	PANTHER	PTHR11685:SF353	RBR-TYPE E3 UBIQUITIN TRANSFERASE	coord: 18..517
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 355..419
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 199..280
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 198..283 e-value: 1.9E-14 score: 55.3
IPR036397	Ribonuclease H superfamily	GENE3D	3.30.420.10		coord: 56..189 e-value: 5.4E-6 score: 28.4
IPR002156	Ribonuclease H domain	PFAM	PF13456	RVT_3	coord: 79..170 e-value: 7.2E-9 score: 35.5
IPR031127	E3 ubiquitin ligase RBR family	PANTHER	PTHR11685	RBR FAMILY RING FINGER AND IBR DOMAIN-CONTAINING	coord: 18..517
IPR017907	Zinc finger, RING-type, conserved site	PROSITE	PS00518	ZF_RING_1	coord: 221..230
IPR044066	TRIAD supradomain	PROSITE	PS51873	TRIAD	coord: 200..417 score: 27.895847

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21444-RA	Carg21444-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032436	positive regulation of proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0000209	protein polyubiquitination
biological_process	GO:0090502	RNA phosphodiester bond hydrolysis, endonucleolytic
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0000151	ubiquitin ligase complex
molecular_function	GO:0004386	helicase activity
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003676	nucleic acid binding
molecular_function	GO:0004523	RNA-DNA hybrid ribonuclease activity
molecular_function	GO:0031624	ubiquitin conjugating enzyme binding
molecular_function	GO:0061630	ubiquitin protein ligase activity
molecular_function	GO:0004842	ubiquitin-protein transferase activity