Carg21331 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21331
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWAT1-related protein
LocationCarg_Chr15: 9375172 .. 9377288 (+)
RNA-Seq ExpressionCarg21331
SyntenyCarg21331
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAGTTTTGTTCAGGTACTTAAATATAATTCACAACTCAACTCAATTCAATTCAACCGTCTATTTTCAGATTGAATTAGATCGAGTGTTCGAGTTGTTTCAATTGTCAACTTTTCCCTGTCAAGAGCAATTGTTGTTACTCTGTTGCTTGCATATATAACGTTTTTTTCAAAATCTCTCTCTGCACTTGTTTTCTAAAACCGAAGCCAGAGACTCGAGCATTCGTTGCTTGGCCATAAGCAACGTAAGAACGAATTGACTTAGCTCACTTGTCATTCGAAAGGAAGTGATGCACAATAGCAAGTTTGCTGCCATAAGAGTGAGATTGTGACAAGTACTCTCCTTATTACATTAATAACTAAAATATTATAAAATTTAGTTGACAAAAATAAAAAATAATTGGGTTGGTTCATTTAAGTTATCCGGTCATATAAACACTCCTATGTTCATGTCGGTTGTTTTACAGTTTTTTTTTCAATTTTAATTTTGGGAAACTTTTTCCAATTTTTAAAATGTGGGTGTAGTTGTACTAAAATTCCATTTTTACACGCATTTTGTCTCATTTTTTTTCATTTTGTCGATAAATATGAATCATTCAAAAGTACTTCTAAATTTATTATTATTATTATTATTTTAAAATTGACAACCATAGAAATGGGAACTTTTCTACTTTACAAAAGCTAAAATATCTTCCATCCTAAACAATATTGATATTGATTTAAAGGTGATGTATTTTCTTGAGCGAATCCTTACTTTTAATTTTAATCGAATACATTTTCAAAAGTAATATTTTTAAAGATATATTCGAACTCGAGGGCATACATTTTGTGGATGTGACATACTTTTACCGAATAATATTTATATTTATAATTTTTTGATGATATTTAAATATATATATATATATATTTTTTTTTTTGTAAGAGTTCAAGTTCATTCCTAACAAATATTGTTCTTTTTGGATGTATTCAGTTAAATATTTTTAATATTTTTATTACTTATGTATAAAGATTGGAGAAAGTGAATATCAAAACTGCGAGAGGTGGTGCCAAAGTGTTGGGAACGGTTATCTGCATTGGCGGTTCCCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCGCTCATAAACATATACAAGAATGAGGACTCTGGGAATAGCAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGGTAACTTAGTCTTATTTTATGGAACCAAATGAAATATGCTCATCAAATCTAAATGGTTTGCAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTTGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATACAGTGTGAGTGTATTGTCTCGTATAGGATACAAGCTATCAACTAACTTCTAAACAAACTATAACTGATTTAATGAGTCATTCGTACTTATGTGTAGGGCATTATTATTTCGGCCGTGGGGTATTTTGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTTAACCCCCTAATGCTGATATTTGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCATGTGGGCAGGTAAGCCACTTACTTCCTCTACTATGTTTGTATTGAATTGAAGGTGTTAACTGAAACTGTTGCGTTATGAAGCCTGGTAGGAGCTTTCTTTATCGTATTGGGTCTGTATTTGGTGCTGTGGGGGAAGAAGGCAGATCATGTGGTGGCGGATGAACAGCAGGAGAAGGAAAAGGTGTTCTTTGACACTGAAATGCAGAGTACGTCTGTAAATGATAATGAAGTGAAAGATAGTGAAAGAGAAACGAGAATTTCTCCGATTTGAGCTGCAAATGTTGCAAGTTAACTACAGAACACACCATGTGGCAGCACTTTGTTCTTTCACTGTTCTTGTATTGTATCTATACTTTTATAAGTCTATATTTAGAATGGAGTTATATACCTGTAGAATTAGC

mRNA sequence

ATGGCAGTTTTGTTCAGATTGGAGAAAGTGAATATCAAAACTGCGAGAGGTGGTGCCAAAGTGTTGGGAACGGTTATCTGCATTGGCGGTTCCCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCGCTCATAAACATATACAAGAATGAGGACTCTGGGAATAGCAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTTGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATACAGTGGCATTATTATTTCGGCCGTGGGGTATTTTGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTTAACCCCCTAATGCTGATATTTGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCATGTGGGCAGCCTGGTAGGAGCTTTCTTTATCGTATTGGGTCTGTATTTGGTGCTGTGGGGGAAGAAGGCAGATCATGTGGTGGCGGATGAACAGCAGGAGAAGGAAAAGGTGTTCTTTGACACTGAAATGCAGAGTACGTCTGTAAATGATAATGAAGTGAAAGATAGTGAAAGAGAAACGAGAATTTCTCCGATTTGAGCTGCAAATGTTGCAAGTTAACTACAGAACACACCATGTGGCAGCACTTTGTTCTTTCACTGTTCTTGTATTGTATCTATACTTTTATAAGTCTATATTTAGAATGGAGTTATATACCTGTAGAATTAGC

Coding sequence (CDS)

ATGGCAGTTTTGTTCAGATTGGAGAAAGTGAATATCAAAACTGCGAGAGGTGGTGCCAAAGTGTTGGGAACGGTTATCTGCATTGGCGGTTCCCTTATTTTCACGTTCTGGAAAGGACCATACTTAACCAAAGCCATCTTCAAGGAGCCGCTCATAAACATATACAAGAATGAGGACTCTGGGAATAGCAGCGGCAAGGATTGGATCAAGGGCTCGGCTTTTATTGTTCTAAGTGAGATGTCTTGGAGTGCATGGCTAATTCTACAGACATTTGTCACCAGAAAATACCCAGCTAAATTGACCATTACTGTTCTAACTTGCGTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGTTCTTTGCAAGAGATCCAGAAAAATGGAAGCTGCAATGGGATGCACGGTTGTTAACCATCGTATACAGTGGCATTATTATTTCGGCCGTGGGGTATTTTGTACAAACCTGGGTCATCAGCCACAATGGGCCAGTTTTTACTTCAATATTTAACCCCCTAATGCTGATATTTGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCATGTGGGCAGCCTGGTAGGAGCTTTCTTTATCGTATTGGGTCTGTATTTGGTGCTGTGGGGGAAGAAGGCAGATCATGTGGTGGCGGATGAACAGCAGGAGAAGGAAAAGGTGTTCTTTGACACTGAAATGCAGAGTACGTCTGTAAATGATAATGAAGTGAAAGATAGTGAAAGAGAAACGAGAATTTCTCCGATTTGA

Protein sequence

MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKDSERETRISPI
Homology
BLAST of Carg21331 vs. NCBI nr
Match: KAG7017068.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 501.1 bits (1289), Expect = 5.5e-138
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS
Sbjct: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF
Sbjct: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF
Sbjct: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND
Sbjct: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240

Query: 241 NEVKDSERETRISPI 256
           NEVKDSERETRISPI
Sbjct: 241 NEVKDSERETRISPI 255

BLAST of Carg21331 vs. NCBI nr
Match: XP_022928955.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 500.0 bits (1286), Expect = 1.2e-137
Identity = 254/255 (99.61%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN+DS
Sbjct: 114 MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF
Sbjct: 174 GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF
Sbjct: 234 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 353

Query: 241 NEVKDSERETRISPI 256
           NEVKDSERETRISPI
Sbjct: 354 NEVKDSERETRISPI 368

BLAST of Carg21331 vs. NCBI nr
Match: KAG6579609.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 491.5 bits (1264), Expect = 4.4e-135
Identity = 249/250 (99.60%), Postives = 250/250 (100.00%), Query Frame = 0

Query: 6   RLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNSSG 65
           +LEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNSSG
Sbjct: 93  KLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDSGNSSG 152

Query: 66  KDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDP 125
           KDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDP
Sbjct: 153 KDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDP 212

Query: 126 EKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVF 185
           EKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVF
Sbjct: 213 EKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVF 272

Query: 186 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKD 245
           SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKD
Sbjct: 273 SERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVNDNEVKD 332

Query: 246 SERETRISPI 256
           SERETRISPI
Sbjct: 333 SERETRISPI 342

BLAST of Carg21331 vs. NCBI nr
Match: XP_022970057.1 (WAT1-related protein At1g43650-like [Cucurbita maxima])

HSP 1 Score: 486.9 bits (1252), Expect = 1.1e-133
Identity = 249/255 (97.65%), Postives = 250/255 (98.04%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNIKTARG AKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN+DS
Sbjct: 114 MAVLFRLEKVNIKTARGAAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF
Sbjct: 174 GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKL WDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF
Sbjct: 234 FARDPEKWKLHWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVF DTEMQSTSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFVDTEMQSTSVND 353

Query: 241 NEVKDSERETRISPI 256
           NEVKDS RETRIS I
Sbjct: 354 NEVKDSVRETRISRI 368

BLAST of Carg21331 vs. NCBI nr
Match: XP_023520558.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 485.3 bits (1248), Expect = 3.1e-133
Identity = 247/255 (96.86%), Postives = 250/255 (98.04%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN+DS
Sbjct: 114 MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF
Sbjct: 174 GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF
Sbjct: 234 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQ+ EK   DTEMQ+TSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQDNEKGLVDTEMQNTSVND 353

Query: 241 NEVKDSERETRISPI 256
           NEVKDS RETRISPI
Sbjct: 354 NEVKDSVRETRISPI 368

BLAST of Carg21331 vs. ExPASy Swiss-Prot
Match: Q9LPF1 (WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.8e-44
Identity = 87/216 (40.28%), Postives = 134/216 (62.04%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           +A++FRLE VN +     AKV+GTVI +GG++I T +KGP +      +   N +    S
Sbjct: 118 LALIFRLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIE---IVKAAHNSFHGGSS 177

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
              +G+ W+ G+  I+ S  +W+A+ ILQ++  + YPA+L++  L C   T  +   +L 
Sbjct: 178 STPTGQHWVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLI 237

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
             RDP  WK+  D+  L  VYSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A  
Sbjct: 238 MVRDPSAWKIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFL 297

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHV 217
            A V +E++H+GS++GA FIVLGLY V+WGK  D V
Sbjct: 298 GALVLAEKIHLGSIIGAVFIVLGLYSVVWGKSKDEV 330

BLAST of Carg21331 vs. ExPASy Swiss-Prot
Match: Q501F8 (WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2 SV=1)

HSP 1 Score: 177.9 bits (450), Expect = 1.4e-43
Identity = 85/224 (37.95%), Postives = 142/224 (63.39%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIY--KNE 60
           MAV+FR+E VN+K  R  AKV+GT I +GG+++ T +KGP +   +FK    +++   + 
Sbjct: 118 MAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAI--ELFKTAHSSLHGGSSG 177

Query: 61  DSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLA 120
            S  ++ ++W+ G+  ++ S  +W+ + ILQ+F  +KYPA+L++ +  C   T  +   +
Sbjct: 178 TSSETTDQNWVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIAS 237

Query: 121 LFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVA 180
           L   RD   WK+  D+  L  VYSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A
Sbjct: 238 LIMVRDVSAWKVGMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITA 297

Query: 181 IFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQ 223
                V +E++H+GS++GA FIV GLY V+WGK  D V++ E++
Sbjct: 298 FLGVLVLAEKIHLGSIIGAIFIVFGLYSVVWGKAKDEVISVEEK 339

BLAST of Carg21331 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 171.4 bits (433), Expect = 1.3e-41
Identity = 92/251 (36.65%), Postives = 144/251 (57.37%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAI---FKEPLINIYKN 60
           +A+LF +E++ +K+ +G AK++G  +C+GG +I   +KGP L   +   F     + ++N
Sbjct: 115 LALLFGMERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRN 174

Query: 61  EDSGNSSGK-DWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGF 120
                S G   W+KG   ++ S + W  WL+LQ  V + YP+KL  T L C+ ++ QS  
Sbjct: 175 NPGHVSGGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFV 234

Query: 121 LALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIF 180
           +A+   RD   WKL W+ RL+ ++Y G I++ V Y++Q+WVI   GPVF S+F PL L+F
Sbjct: 235 IAIALERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLF 294

Query: 181 VAIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQST 240
             + SA +  E + +GS+VG   +++GLY VLWGK            +EK   D ++   
Sbjct: 295 TLLSSAILLCEIISLGSIVGGLLLIIGLYCVLWGK----------SREEKNSGDDKIDLQ 354

Query: 241 SVND---NEVK 245
             ND   NEVK
Sbjct: 355 KENDVVCNEVK 355

BLAST of Carg21331 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 168.3 bits (425), Expect = 1.1e-40
Identity = 85/246 (34.55%), Postives = 145/246 (58.94%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           +A + R+EKVNI   R  AK++GT++ +GG+L+ T +KGP L    +  P ++      +
Sbjct: 120 IAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGP-LIPLPWSNPNMDQQNGHTN 179

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
            +    +W+ G+  I+L  ++WS + +LQ+   + YPA L+++ L C+    QS  +AL 
Sbjct: 180 NSQDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALV 239

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
             R P  W + WDARL   +Y+GI+ S + Y+VQ  V+   GPVF + FNPL +I VA+ 
Sbjct: 240 VERHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALI 299

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           ++F+  E++H G ++G   I  GLY+V+WGK  D+ V+     ++    +  + + S +D
Sbjct: 300 ASFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKSEDD 359

Query: 241 NEVKDS 247
           N++  S
Sbjct: 360 NKLVSS 364

BLAST of Carg21331 vs. ExPASy Swiss-Prot
Match: F4HZQ7 (WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.5e-40
Identity = 87/231 (37.66%), Postives = 134/231 (58.01%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           +A++FRLE VN K  R  AKV+GTVI + G+L+ T +KGP +    F         ++ +
Sbjct: 118 LAIIFRLESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGG--GGGGSDGA 177

Query: 61  GNSSG--------KDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATA 120
           G S G        K WI G+  ++     W+ + ILQ+F  ++YPA+L++T L C+  T 
Sbjct: 178 GGSHGGAGAAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTL 237

Query: 121 QSGFLALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPL 180
           +   ++L   RD   WK+ +D+ L    YSG+I S V Y+VQ  V+   GPVF + FNPL
Sbjct: 238 EGTAVSLVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPL 297

Query: 181 MLIFVAIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQE 224
            ++  A     V SE +H+GS++G  FI++GLY V+WGK  D  + D+ ++
Sbjct: 298 CVVITAALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDED 346

BLAST of Carg21331 vs. ExPASy TrEMBL
Match: A0A6J1ESW6 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1)

HSP 1 Score: 500.0 bits (1286), Expect = 6.0e-138
Identity = 254/255 (99.61%), Postives = 255/255 (100.00%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN+DS
Sbjct: 114 MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF
Sbjct: 174 GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF
Sbjct: 234 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 353

Query: 241 NEVKDSERETRISPI 256
           NEVKDSERETRISPI
Sbjct: 354 NEVKDSERETRISPI 368

BLAST of Carg21331 vs. ExPASy TrEMBL
Match: A0A6J1HY24 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1)

HSP 1 Score: 486.9 bits (1252), Expect = 5.2e-134
Identity = 249/255 (97.65%), Postives = 250/255 (98.04%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNIKTARG AKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKN+DS
Sbjct: 114 MAVLFRLEKVNIKTARGAAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF
Sbjct: 174 GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKL WDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF
Sbjct: 234 FARDPEKWKLHWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVF DTEMQSTSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFVDTEMQSTSVND 353

Query: 241 NEVKDSERETRISPI 256
           NEVKDS RETRIS I
Sbjct: 354 NEVKDSVRETRISRI 368

BLAST of Carg21331 vs. ExPASy TrEMBL
Match: A0A6J1EQL7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1)

HSP 1 Score: 427.6 bits (1098), Expect = 3.8e-116
Identity = 211/246 (85.77%), Postives = 232/246 (94.31%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNI+TARG AKV+GT++CIGGSLIFT WKGPY+TKAIFKEPLI+IYKN DS
Sbjct: 114 MAVLFRLEKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFI++S+++WS WLILQ FVTRKYPA+LT+TVL C+FATAQSGFLAL 
Sbjct: 174 GNSSGKDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALI 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKLQWDARLLTIVYSGI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFVAIF
Sbjct: 234 FARDPEKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVA E QEK+KVF DTEMQSTSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQSTSVND 353

Query: 241 NEVKDS 247
           ++VK S
Sbjct: 354 SQVKGS 359

BLAST of Carg21331 vs. ExPASy TrEMBL
Match: A0A6J1I2R9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 5.4e-115
Identity = 208/244 (85.25%), Postives = 230/244 (94.26%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MAVLFRLEKVNI+TARG AKV GT++CIGGSLIFT WKGPY+TKAIFKEPLI+IY+N DS
Sbjct: 114 MAVLFRLEKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDS 173

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           GNSSGKDWIKGSAFI++S+++WS WLILQ FVTRKYPA+LT+TVL C+FATAQSGFLAL 
Sbjct: 174 GNSSGKDWIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALI 233

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARDPEKWKLQWDARLLTIVYSGI+IS VGYF+QTW IS NGPVFTS+F+PLMLIFVAIF
Sbjct: 234 FARDPEKWKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIF 293

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVA E QEK+KVF DTEMQ+TSVND
Sbjct: 294 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEPQEKDKVFVDTEMQNTSVND 353

Query: 241 NEVK 245
           ++VK
Sbjct: 354 SQVK 357

BLAST of Carg21331 vs. ExPASy TrEMBL
Match: A0A6J1EQM5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1)

HSP 1 Score: 367.9 bits (943), Expect = 3.5e-98
Identity = 178/220 (80.91%), Postives = 198/220 (90.00%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           MA LFRLE+VNI+TARGGAKVLGT+ CIGGS +FTFWKGPY+TK IFKEPLI++YKN+  
Sbjct: 121 MAFLFRLERVNIRTARGGAKVLGTITCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
           G+ SG+DWIKGSA IV+SE++WSAWLIL T VTRKYPA+LTITVL C+FATAQSGFLAL 
Sbjct: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
           FARD EKWKL WDARLLTIVY G++IS  GYF+QTW IS NGPVFTS+FNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADE 221
           SAFVFSERLHVGSLVGAF I+LGLYLVLWGKKADH VA E
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPE 340

BLAST of Carg21331 vs. TAIR 10
Match: AT1G44800.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 179.5 bits (454), Expect = 3.4e-45
Identity = 87/216 (40.28%), Postives = 134/216 (62.04%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           +A++FRLE VN +     AKV+GTVI +GG++I T +KGP +      +   N +    S
Sbjct: 118 LALIFRLETVNFRKVHSVAKVVGTVITVGGAMIMTLYKGPAIE---IVKAAHNSFHGGSS 177

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
              +G+ W+ G+  I+ S  +W+A+ ILQ++  + YPA+L++  L C   T  +   +L 
Sbjct: 178 STPTGQHWVLGTIAIMGSISTWAAFFILQSYTLKVYPAELSLVTLICGIGTILNAIASLI 237

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
             RDP  WK+  D+  L  VYSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A  
Sbjct: 238 MVRDPSAWKIGMDSGTLAAVYSGVVCSGIAYYIQSIVIKQRGPVFTTSFSPMCMIITAFL 297

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHV 217
            A V +E++H+GS++GA FIVLGLY V+WGK  D V
Sbjct: 298 GALVLAEKIHLGSIIGAVFIVLGLYSVVWGKSKDEV 330

BLAST of Carg21331 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 177.9 bits (450), Expect = 1.0e-44
Identity = 85/224 (37.95%), Postives = 142/224 (63.39%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIY--KNE 60
           MAV+FR+E VN+K  R  AKV+GT I +GG+++ T +KGP +   +FK    +++   + 
Sbjct: 118 MAVIFRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAI--ELFKTAHSSLHGGSSG 177

Query: 61  DSGNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLA 120
            S  ++ ++W+ G+  ++ S  +W+ + ILQ+F  +KYPA+L++ +  C   T  +   +
Sbjct: 178 TSSETTDQNWVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIAS 237

Query: 121 LFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVA 180
           L   RD   WK+  D+  L  VYSG++ S + Y++Q+ VI   GPVFT+ F+P+ +I  A
Sbjct: 238 LIMVRDVSAWKVGMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITA 297

Query: 181 IFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQ 223
                V +E++H+GS++GA FIV GLY V+WGK  D V++ E++
Sbjct: 298 FLGVLVLAEKIHLGSIIGAIFIVFGLYSVVWGKAKDEVISVEEK 339

BLAST of Carg21331 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 171.4 bits (433), Expect = 9.4e-43
Identity = 92/251 (36.65%), Postives = 144/251 (57.37%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAI---FKEPLINIYKN 60
           +A+LF +E++ +K+ +G AK++G  +C+GG +I   +KGP L   +   F     + ++N
Sbjct: 115 LALLFGMERLKVKSIQGTAKLVGITVCMGGVIILAIYKGPLLKLPLCPHFYHGQEHPHRN 174

Query: 61  EDSGNSSGK-DWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGF 120
                S G   W+KG   ++ S + W  WL+LQ  V + YP+KL  T L C+ ++ QS  
Sbjct: 175 NPGHVSGGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFV 234

Query: 121 LALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIF 180
           +A+   RD   WKL W+ RL+ ++Y G I++ V Y++Q+WVI   GPVF S+F PL L+F
Sbjct: 235 IAIALERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLF 294

Query: 181 VAIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQST 240
             + SA +  E + +GS+VG   +++GLY VLWGK            +EK   D ++   
Sbjct: 295 TLLSSAILLCEIISLGSIVGGLLLIIGLYCVLWGK----------SREEKNSGDDKIDLQ 354

Query: 241 SVND---NEVK 245
             ND   NEVK
Sbjct: 355 KENDVVCNEVK 355

BLAST of Carg21331 vs. TAIR 10
Match: AT4G08290.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 168.3 bits (425), Expect = 7.9e-42
Identity = 85/246 (34.55%), Postives = 145/246 (58.94%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           +A + R+EKVNI   R  AK++GT++ +GG+L+ T +KGP L    +  P ++      +
Sbjct: 120 IAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVMTLYKGP-LIPLPWSNPNMDQQNGHTN 179

Query: 61  GNSSGKDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALF 120
            +    +W+ G+  I+L  ++WS + +LQ+   + YPA L+++ L C+    QS  +AL 
Sbjct: 180 NSQDHNNWVVGTLLILLGCVAWSGFYVLQSITIKTYPADLSLSALICLAGAVQSFAVALV 239

Query: 121 FARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIF 180
             R P  W + WDARL   +Y+GI+ S + Y+VQ  V+   GPVF + FNPL +I VA+ 
Sbjct: 240 VERHPSGWAVGWDARLFAPLYTGIVSSGITYYVQGMVMKTRGPVFVTAFNPLCMILVALI 299

Query: 181 SAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQEKEKVFFDTEMQSTSVND 240
           ++F+  E++H G ++G   I  GLY+V+WGK  D+ V+     ++    +  + + S +D
Sbjct: 300 ASFILHEQIHFGCVIGGAVIAAGLYMVVWGKGKDYEVSGLDILEKNSLQELPITTKSEDD 359

Query: 241 NEVKDS 247
           N++  S
Sbjct: 360 NKLVSS 364

BLAST of Carg21331 vs. TAIR 10
Match: AT1G21890.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 167.9 bits (424), Expect = 1.0e-41
Identity = 87/231 (37.66%), Postives = 134/231 (58.01%), Query Frame = 0

Query: 1   MAVLFRLEKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNEDS 60
           +A++FRLE VN K  R  AKV+GTVI + G+L+ T +KGP +    F         ++ +
Sbjct: 118 LAIIFRLESVNFKKVRSIAKVVGTVITVSGALLMTLYKGPIVDFIRFGGG--GGGGSDGA 177

Query: 61  GNSSG--------KDWIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATA 120
           G S G        K WI G+  ++     W+ + ILQ+F  ++YPA+L++T L C+  T 
Sbjct: 178 GGSHGGAGAAAMDKHWIPGTLMLLGRTFGWAGFFILQSFTLKQYPAELSLTTLICLMGTL 237

Query: 121 QSGFLALFFARDPEKWKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPL 180
           +   ++L   RD   WK+ +D+ L    YSG+I S V Y+VQ  V+   GPVF + FNPL
Sbjct: 238 EGTAVSLVTVRDLSAWKIGFDSNLFAAAYSGVICSGVAYYVQGVVMRERGPVFVATFNPL 297

Query: 181 MLIFVAIFSAFVFSERLHVGSLVGAFFIVLGLYLVLWGKKADHVVADEQQE 224
            ++  A     V SE +H+GS++G  FI++GLY V+WGK  D  + D+ ++
Sbjct: 298 CVVITAALGVVVLSESIHLGSVIGTLFIIVGLYTVVWGKGKDKRMTDDDED 346

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017068.15.5e-138100.00WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022928955.11.2e-13799.61WAT1-related protein At1g43650-like [Cucurbita moschata][more]
KAG6579609.14.4e-13599.60WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022970057.11.1e-13397.65WAT1-related protein At1g43650-like [Cucurbita maxima][more]
XP_023520558.13.1e-13396.86WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9LPF14.8e-4440.28WAT1-related protein At1g44800 OS=Arabidopsis thaliana OX=3702 GN=At1g44800 PE=1... [more]
Q501F81.4e-4337.95WAT1-related protein At4g08300 OS=Arabidopsis thaliana OX=3702 GN=At4g08300 PE=2... [more]
Q9FGG31.3e-4136.65WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2... [more]
Q9SUF11.1e-4034.55WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2... [more]
F4HZQ71.5e-4037.66WAT1-related protein At1g21890 OS=Arabidopsis thaliana OX=3702 GN=At1g21890 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1ESW66.0e-13899.61WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1[more]
A0A6J1HY245.2e-13497.65WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1[more]
A0A6J1EQL73.8e-11685.77WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1[more]
A0A6J1I2R95.4e-11585.25WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1[more]
A0A6J1EQM53.5e-9880.91WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G44800.13.4e-4540.28nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08300.11.0e-4437.95nodulin MtN21 /EamA-like transporter family protein [more]
AT5G64700.19.4e-4336.65nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08290.17.9e-4234.55nodulin MtN21 /EamA-like transporter family protein [more]
AT1G21890.11.0e-4137.66nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 70..208
e-value: 2.3E-16
score: 60.2
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 235..255
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 241..255
NoneNo IPR availablePANTHERPTHR31218:SF265WAT1-RELATED PROTEINcoord: 1..226
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 116..212
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 1..226

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21331-RACarg21331-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity