Carg21220 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21220
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	4-coumarate--CoA ligase-like 1
Location	Carg_Chr18: 7678813 .. 7681426 (+)
RNA-Seq Expression	Carg21220
Synteny	Carg21220

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTAATGAGTGGCATGAATCAACAACATCCCACCAAAAAAAGGCTCATGCCAATCTCACCCTCAGTAGCTCTACAAATTTCTCATCCACAAAGCATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTCAGTCGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCCGAGGTCCAAGTGCCTGATGATATCACATTGCCAGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTAAAATCGAAACTGTCGTTATCGATCATATTATTTCGAATTCAACTCGACTTGCTTACTATCTAATGATTGATAACAGGTGAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACCAACTGCTCGAAGCTGCGGACCGCGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCAAACTTGTGTTCTACGCTCTCCGGCGTACCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGTAATTAAGTTACTACTCGAGTAGATGGATTGTGAGAACTCTCGACTCTTATTCATTCTGTCAAATTATCAGGCTATAACATGTAGTCCACCGTCCTGTTTTCTGCATATCGAAAACGAACCGAGCTCCACCGTTGCATTGTAGAGCTGATTCACAGGGTCATTCTATCTAGGCTAACTAAATGTCATTGATCGCCAACAGGCATATGGGCTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCGCTGCAAAGAAAAACACGGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCCGACAGCGGTAGGTCGCTGCCGAAGAACACACCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGTACATAATTTGGACTGGTGCAGAAAATCCATCTGAGATCTCTAATTATATGCTAGTTGAATCTTTCCTTGTTGTTTCAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAAAGACACTGCATAAACTTTGCTTGATAAATCGAAGCTCGAGAACAGAAAAAAAGTAGAATTCAAATGGTTATCAAACGAGACCTATATAAGGTAACGAATGTCTTTGATTTCTCTACAGGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGTAAGCTTCGCTTCCCTTTACATATTTCTATTTAAATGATTTCTGAAACAAATCGAACCCGAAACGGTTAAAACAACGTTAACCCGCGTCAGTATTTCAGGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGACTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGACGGCCTCTTGATCCGTGGCTGTGATGGCTATATTTTGTTTGAATCCTCCATTGCAATGGGCAGTGAAGCTCACACTAAGCTCTTTTTCTTTCCCTTTCTCAAGTGCCTAGTTTGGCTGTTCTGTTCTGTTAGGCCTCAATGCAAGTTGTTGTTTAATAATATGAAAGCTTATATTTTAATTTCTATTTTTCAATAAATAAGTTTATTTTTCTTTTTCCT

mRNA sequence

TTAATGAGTGGCATGAATCAACAACATCCCACCAAAAAAAGGCTCATGCCAATCTCACCCTCAGTAGCTCTACAAATTTCTCATCCACAAAGCATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTCAGTCGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCCGAGGTCCAAGTGCCTGATGATATCACATTGCCAGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTGAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACCAACTGCTCGAAGCTGCGGACCGCGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCAAACTTGTGTTCTACGCTCTCCGGCGTACCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGGCTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCGCTGCAAAGAAAAACACGGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCCGACAGCGGTAGGTCGCTGCCGAAGAACACACCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGACTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGACGGCCTCTTGATCCGTGGCTGTGATGGCTATATTTTGTTTGAATCCTCCATTGCAATGGGCAGTGAAGCTCACACTAAGCTCTTTTTCTTTCCCTTTCTCAAGTGCCTAGTTTGGCTGTTCTGTTCTGTTAGGCCTCAATGCAAGTTGTTGTTTAATAATATGAAAGCTTATATTTTAATTTCTATTTTTCAATAAATAAGTTTATTTTTCTTTTTCCT

Coding sequence (CDS)

ATGAGTGGCATGAATCAACAACATCCCACCAAAAAAAGGCTCATGCCAATCTCACCCTCAGTAGCTCTACAAATTTCTCATCCACAAAGCATGATACAAGCTTTCTCAGCTCCTCCTTTAAGAAGAGGGAGACGCCCGTCGATGTCTGCAGCAAACAACAACTCCGCCATGGCTACTTGCATTCGGGATTCAGTCGAAGACGAGGAACACATTTTCCGTAGCCAACTTCCCGAGGTCCAAGTGCCTGATGATATCACATTGCCAGAGTTTGTACTTCAGAATGCTGAATCATATGCTGATAATGTGGCATTTGTGGAAGCTGTGAGCGGAAAGGCGTACACTTATCGTGAAGTTGTGAGAGACACGAGTAGGTTCGCTAAGGCCTTAAGCTCTCTGAGGTTGAAGAAGGGGCAGGTCGTTATTGTCGTTCTACCCAACGTTGCTGAATATGCCATTGTTGCTTTAGGGATAATGGCTGCTGGAGGTGTGTTTTCTGGTGTGAATCCAGCAGCTCACATATCCGAAATCAAAAAGCAGGTGGAGGTAGCAGACGCCAAACTCGTCGTTACAAACAGCTCAAGCTTTGAAAAGGTGAAGGAATTAAAGCTTCCAGTGATCGTAGTAGGGGAGGAACTGATTGAAGGTTCCATGAACTGGCACCAACTGCTCGAAGCTGCGGACCGCGCGGGCAACAATTTCGTCAAAGAAGATATCAAGCAGACTGATTTATGTGCCCTTCCTTTCTCATCAGGCACTACAGGAGTTTCCAAAGGTGTAATGCTAACTCATCGAAATCTAGTGGCAAACTTGTGTTCTACGCTCTCCGGCGTACCGCAAGAAATGGTGGGCAAGGTCACGACGTTAGGCCTCATCCCGTTCTTCCACATTTACGGGATTACCGGAATATGTTGTGCCACACTTCGAAACAAGGGAAAAGTTGTGGTGATGGGAAGATTTGATCTTAGAACCTTCATCAATGCCCTGATAACACAGGAGGTCACATTTGCTCCAATTGTTCCTCCTATCATCTTGGCCTTGGTTAAGAATCCTATTGTGAAGGAATTCGATCTAAGCGGTCTCAAACTTCAGGCTATCATGACTGCAGCTGCTCCGCTCGCACCCGAACTTCAAACGTCCTTTGAGAAAAAGTTCCCGGGCGTGGATGTTCAAGAGGCATATGGGCTAACCGAGCATAGCTGCATCACTCTCAACTATGGAAGCATAGGCAAAGACAATCTCGCTGCAAAGAAAAACACGGTTGGCCGTATTCTTCCTAATCTAGAAGTCAAATTCATCGACCCCGACAGCGGTAGGTCGCTGCCGAAGAACACACCTGGGGAAATCTGTGTAAGAAGCCAGTGTGTGATGCAAGGCTACTACAATAATGAAGAGGAGACTTCAAGGACCATCGACGACAAAGGCTGGATGCACACGGGCGACATCGGATACATCGACGACGATGGCGATGTGTTTATTGTGGATCGTATTAAAGAGTTGATTAAGTACAAAGGCTTTCAAGTAGCTCCGGCCGAGTTAGAGGCAATCCTCCTTACTCATCCCTCGATTGAAGATGCAGCTGTGGTGCCGCTGCCAGACAAAGAAGCCGGTGAGATCCCAGCAGCAAGCATTGTAATGACTCCAAATGCAAAAGAAACCGAAGACGAAATAATCAAATACGTCGCCTCAAACGTTGCACATTACAAGAAAGTTAGGCTCGTTCATTTTGTCGAAACGATCCCGAAATCGCCGTCTGGGAAAGTCATGAGGAGGTTGATCAAAGAGAAGATGATCGAAAAGATTCGAGCCGAGACGGCCTCTTGA

Protein sequence

MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS

Homology

[Close]

BLAST of Carg21220 vs. NCBI nr
Match: KAG7012722.1 (4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1176.4 bits (3042), Expect = 0.0e+00
Identity = 606/606 (100.00%), Postives = 606/606 (100.00%), Query Frame = 0


Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60

Query: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAETAS 607
           RAETAS
Sbjct: 601 RAETAS 606

[Close]

BLAST of Carg21220 vs. NCBI nr
Match: XP_022945246.1 (4-coumarate--CoA ligase-like 1 [Cucurbita moschata])

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 602/606 (99.34%), Postives = 603/606 (99.50%), Query Frame = 0


Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60

Query: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWH+LLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVM PNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAETAS 607
           RAE AS
Sbjct: 601 RAEAAS 606

[Close]

BLAST of Carg21220 vs. NCBI nr
Match: XP_023541569.1 (4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1163.3 bits (3008), Expect = 0.0e+00
Identity = 600/606 (99.01%), Postives = 601/606 (99.17%), Query Frame = 0


Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRR  MSAA NNSAMATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRSLMSAATNNSAMATC 60

Query: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSG AYTYREVVR
Sbjct: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGNAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWH+LLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVM PNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAETAS 607
           RAETAS
Sbjct: 601 RAETAS 606

[Close]

BLAST of Carg21220 vs. NCBI nr
Match: XP_022966799.1 (4-coumarate--CoA ligase-like 1 [Cucurbita maxima])

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 595/606 (98.18%), Postives = 600/606 (99.01%), Query Frame = 0


Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRR SMSAANNNS MATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATC 60

Query: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRDS EDEEHIFRSQLPEVQVPDDITLPEFVLQNAE YADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWH+LLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLS L
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYG+IGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID+KGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVM PNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAETAS 607
           RAETAS
Sbjct: 601 RAETAS 606

[Close]

BLAST of Carg21220 vs. NCBI nr
Match: KAG6573594.1 (4-coumarate--CoA ligase-like 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1065.1 bits (2753), Expect = 2.3e-307
Identity = 554/576 (96.18%), Postives = 556/576 (96.53%), Query Frame = 0


Query: 31  MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 90
           MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF
Sbjct: 1   MIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEF 60

Query: 91  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 150
           VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY
Sbjct: 61  VLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEY 120

Query: 151 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 210
           AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE
Sbjct: 121 AIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGE 180

Query: 211 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 270
           ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL
Sbjct: 181 ELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANL 240

Query: 271 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 330
           CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT
Sbjct: 241 CSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALIT 300

Query: 331 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 390
           QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ
Sbjct: 301 QEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQ 360

Query: 391 EAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICV 450
           EAYGLTEHSCITLNYGSIGKDNL  ++ T                 GRSLPKNTPGEICV
Sbjct: 361 EAYGLTEHSCITLNYGSIGKDNLTLQRKT----------------RGRSLPKNTPGEICV 420

Query: 451 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 510
           RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA
Sbjct: 421 RSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPA 480

Query: 511 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 570
           ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV
Sbjct: 481 ELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKV 540

Query: 571 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 607
           RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS
Sbjct: 541 RLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 560

[Close]

BLAST of Carg21220 vs. ExPASy Swiss-Prot
Match: Q9LQ12 (4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=1)

HSP 1 Score: 788.9 bits (2036), Expect = 4.1e-227
Identity = 389/532 (73.12%), Postives = 459/532 (86.28%), Query Frame = 0


Query: 66  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRF 125
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 126 AKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 185
           AKAL+SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 186 KLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCA 245
           + ++T+++++EKVK L LPVIV+GEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 246 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 305
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 306 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 365
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 366 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILP 425
           MTAAAPLAPEL T+FE KFP V VQEAYGLTEHSCITL +G   K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 426 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 485
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID++GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 486 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 545
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPD+EAGEIPAA +V+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 546 TPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMI 598
            P A E E++I+ +VA+NVAHYKKVR VHFV++IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

[Close]

BLAST of Carg21220 vs. ExPASy Swiss-Prot
Match: Q7XXL2 (4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9 PE=2 SV=2)

HSP 1 Score: 701.4 bits (1809), Expect = 8.7e-201
Identity = 347/539 (64.38%), Postives = 440/539 (81.63%), Query Frame = 0


Query: 66  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVS-GKAYTYREVVRDTSR 125
           E++EH+FRS+ P V VPD +T+PEFVL  AE+YAD VA VEA + G++YTY EV RDT+R
Sbjct: 14  EEQEHVFRSRFPPVAVPDGVTVPEFVLDGAEAYADRVALVEAAAGGRSYTYGEVARDTAR 73

Query: 126 FAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVAD 185
           FA+AL S+ ++KG VV+V LPN+A Y +V+LGIM+AG VFSGVNP A  +EIKKQVE ++
Sbjct: 74  FARALRSVGVRKGHVVVVALPNLAVYPVVSLGIMSAGAVFSGVNPRALAAEIKKQVEDSE 133

Query: 186 AKLVVTNSSSFEKVKELKLPVIVVGE-ELIEGSMNWHQLLEAADRAGNNFVKED-IKQTD 245
           AKLVV N  +F+KVK+  +PVI VG+ E + G+++W  LL AADR G   V  D  +Q+D
Sbjct: 134 AKLVVANEVAFDKVKDAGVPVIGVGDRERMPGAISWDGLLAAADRTGAGVVPVDAAQQSD 193

Query: 246 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGI 305
           LCALP+SSGTTGVSKGVML+HRNLV+NLCS++  V  E  G+V TLGL+PFFHIYGITGI
Sbjct: 194 LCALPYSSGTTGVSKGVMLSHRNLVSNLCSSMFAVAPETAGQVVTLGLMPFFHIYGITGI 253

Query: 306 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 365
           CCATLR+KG VVVM RFDLRTF+ AL+   V FAP+VPP++LA+VK+P+  EFDLS L L
Sbjct: 254 CCATLRHKGTVVVMDRFDLRTFLRALVDHRVMFAPLVPPVMLAMVKSPVADEFDLSDLAL 313

Query: 366 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGR 425
           +++MTAAAPLAP+L  +F++KFPGV V+EAYGLTEHSCITL + +       AKK++VG 
Sbjct: 314 KSVMTAAAPLAPDLLAAFQRKFPGVQVEEAYGLTEHSCITLTHAAGDGHGHVAKKSSVGF 373

Query: 426 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 485
           ILPNLEVKF+DPD+GRSLP NTPGE+CVRSQ VMQGYY  +EET RT+D KGW+HTGD+G
Sbjct: 374 ILPNLEVKFVDPDTGRSLPANTPGELCVRSQSVMQGYYKRKEETERTVDGKGWLHTGDVG 433

Query: 486 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 545
           YID DGDVFIVDRIKELIKYKGFQVAPAELEA+LL+HPS+EDAAV  +PD+EAGE+P A 
Sbjct: 434 YIDGDGDVFIVDRIKELIKYKGFQVAPAELEAVLLSHPSVEDAAVFGVPDEEAGEVPVAC 493

Query: 546 IVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIR 602
           +V    A+E E+EI+ YVA  VA YK+VR++H V+ IPKS SGK++RR ++++ I++++
Sbjct: 494 VVRRHGAEEGEEEIVAYVAERVASYKRVRVLHIVDAIPKSVSGKILRRQLRDEFIKRMK 552

[Close]

BLAST of Carg21220 vs. ExPASy Swiss-Prot
Match: P14912 (4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.5e-112
Identity = 208/534 (38.95%), Postives = 332/534 (62.17%), Query Frame = 0


Query: 68  EEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAK 127
           E+ IFRS+LP++ +P  + L  +  +N     D    +   +G+ +TY +V   + + A 
Sbjct: 9   EDLIFRSKLPDIYIPKHLPLHTYCFENISKVGDKSCLINGATGETFTYSQVELLSRKVAS 68

Query: 128 ALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKL 187
            L+ L +++G  ++++LPN  EY    LG    G + +  NP    +E+ KQ++ + AKL
Sbjct: 69  GLNKLGIQQGDTIMLLLPNSPEYFFAFLGASYRGAISTMANPFFTSAEVIKQLKASQAKL 128

Query: 188 VVTNSSSFEKVKEL---KLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLC 247
           ++T +   +KVK+    K   I+  ++  +  +++ +L+EA +      V   I   D+ 
Sbjct: 129 IITQACYVDKVKDYAAEKNIQIICIDDAPQDCLHFSKLMEADESEMPEVV---INSDDVV 188

Query: 248 ALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGI 307
           ALP+SSGTTG+ KGVMLTH+ LV ++   + G      M  +   + ++P FHIY +  +
Sbjct: 189 ALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGDNPNLYMHSEDVMICILPLFHIYSLNAV 248

Query: 308 CCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKL 367
            C  LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V ++DLS ++ 
Sbjct: 249 LCCGLRAGVTILIMQKFDIVPFLELIQKYKVTIGPFVPPIVLAIAKSPVVDKYDLSSVR- 308

Query: 368 QAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGR 427
             +M+ AAPL  EL+ +   KFP   + + YG+TE   +     +  K+    K    G 
Sbjct: 309 -TVMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPYEIKSGACGT 368

Query: 428 ILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIG 487
           ++ N E+K +DP++  SLP+N  GEIC+R   +M+GY N+ E T  TID++GW+HTGDIG
Sbjct: 369 VVRNAEMKIVDPETNASLPRNQRGEICIRGDQIMKGYLNDPESTRTTIDEEGWLHTGDIG 428

Query: 488 YIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAAS 547
           +IDDD ++FIVDR+KE+IKYKGFQVAPAELEA+LLTHP+I DAAVVP+ D++AGE+P A 
Sbjct: 429 FIDDDDELFIVDRLKEIIKYKGFQVAPAELEALLLTHPTISDAAVVPMIDEKAGEVPVAF 488

Query: 548 IVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           +V T     TE+EI ++V+  V  YK++  V FV+ IPKSPSGK++R+ ++ ++
Sbjct: 489 VVRTNGFTTTEEEIKQFVSKQVVFYKRIFRVFFVDAIPKSPSGKILRKDLRARI 537

[Close]

BLAST of Carg21220 vs. ExPASy Swiss-Prot
Match: P31684 (4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1)

HSP 1 Score: 408.3 bits (1048), Expect = 1.5e-112
Identity = 206/532 (38.72%), Postives = 330/532 (62.03%), Query Frame = 0


Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRS+LP++ +P  + L  +  +N   +      ++  + + YTY EV   + + A  L+
Sbjct: 14  IFRSKLPDIYIPKHLPLHSYCFENLSEFNSRPCLIDGANDRIYTYAEVELTSRKVAVGLN 73

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
            L +++   ++++LPN  E+    +G    G + +  NP    +E+ KQ + + AK+V+T
Sbjct: 74  KLGIQQKDTIMILLPNCPEFVFAFIGASYLGAISTMANPLFTPAEVVKQAKASSAKIVIT 133

Query: 191 NSSSFEKVK----ELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCAL 250
            +    KVK    E  L VI V + + EG +++ +L+++ +   +      I+  D+ AL
Sbjct: 134 QACFAGKVKDYAIENDLKVICV-DSVPEGCVHFSELIQSDE---HEIPDVKIQPDDVVAL 193

Query: 251 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQE--MVGKVTTLGLIPFFHIYGITGICC 310
           P+SSGTTG+ KGVMLTH+ LV ++   + G      M      + ++P FHIY +  +  
Sbjct: 194 PYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENANLYMHSDDVLMCVLPLFHIYSLNSVLL 253

Query: 311 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQA 370
             LR    +++M +FD+  F+  +   +VT  P VPPI+LA+ K+P+V  +DLS ++   
Sbjct: 254 CALRVGAAILIMQKFDIAQFLELIPKHKVTIGPFVPPIVLAIAKSPLVDNYDLSSVR--T 313

Query: 371 IMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 430
           +M+ AAPL  EL+ +   KFP   + + YG+TE   +     +  K+    K    G ++
Sbjct: 314 VMSGAAPLGKELEDAVRAKFPNAKLGQGYGMTEAGPVLAMCLAFAKEPFDIKSGACGTVV 373

Query: 431 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYI 490
            N E+K +DPD+G SLP+N PGEIC+R   +M+GY N+ E T+RTI+ +GW+HTGDIG+I
Sbjct: 374 RNAEMKIVDPDTGCSLPRNQPGEICIRGDQIMKGYLNDPEATARTIEKEGWLHTGDIGFI 433

Query: 491 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIV 550
           DDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I DAAVVP+ D++AGE+P A +V
Sbjct: 434 DDDDELFIVDRLKELIKYKGFQVAPAELEALLINHPDISDAAVVPMIDEQAGEVPVAFVV 493

Query: 551 MTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
            +  +  TEDE+  +++  V  YK+++ V FVET+PKSPSGK++R+ ++ ++
Sbjct: 494 RSNGSTITEDEVKDFISKQVIFYKRIKRVFFVETVPKSPSGKILRKDLRARL 539

[Close]

BLAST of Carg21220 vs. ExPASy Swiss-Prot
Match: A0A2H5AIX5 (4-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 2.6e-112
Identity = 214/536 (39.93%), Postives = 330/536 (61.57%), Query Frame = 0


Query: 67  DEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFA 126
           ++E IFRS+LP++ VPD + L  +  QN   ++D    ++  + K  TY EV   + R  
Sbjct: 7   EKETIFRSKLPDIYVPDHLPLHSYCFQNLHQFSDRPCLIDGFTNKTLTYAEVELASKRVG 66

Query: 127 KALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAK 186
             L  L L++G VV+++LPN  E+ +  +G    G + +  NP    +EI KQ   A AK
Sbjct: 67  AGLHRLGLRQGHVVMLLLPNSIEFVLSFIGASLLGAMSTTANPFYTSAEIHKQAAAAGAK 126

Query: 187 LVVTNSSSFEKVKELK-LPVIVVGEELI---EGSMNWHQLLEAADRAGNNFVKEDIKQTD 246
           ++VT S    K++ L+ +  IVV ++ +   E  M++ + LE+ D A     + D+   D
Sbjct: 127 IIVTESCHVSKLQGLEGISRIVVIDDAVRVPENVMHFSE-LESTDEA--ELPRIDVHPDD 186

Query: 247 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--VGKVTTLGLIPFFHIYGIT 306
           + ALP+SSGTTG+ KGVMLTH  LV ++   + G    +        L ++P FHIY + 
Sbjct: 187 VVALPYSSGTTGLPKGVMLTHNGLVTSVAQQVDGENPNLHFSEDDVLLCVLPLFHIYSLN 246

Query: 307 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 366
            +    LR    +V+M +F++   +  +    VT AP VPPI++ +VKN  V  +DLS +
Sbjct: 247 SVLLCGLRAGAAIVLMRKFEIVRLMELVEKYRVTIAPFVPPIVVEMVKNEAVDRYDLSSI 306

Query: 367 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 426
           ++  +M+ AAP+  EL+    +K P   + + YG+TE   +     +  K+    K  + 
Sbjct: 307 RV--VMSGAAPMGKELENKLREKLPNAKLGQGYGMTEAGPVLSMCLAFAKEPFEVKSGSC 366

Query: 427 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 486
           G ++ N E+K IDP++G SL +N PGEIC+R   +M+GY NN E T +TID++GW+HTGD
Sbjct: 367 GTVVRNAELKIIDPETGFSLSRNQPGEICIRGNQIMKGYLNNPEATKQTIDEEGWLHTGD 426

Query: 487 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 546
           IG++DDD ++FIVDR+KELIKYKGFQVAPAELEA+L+THP++ DAAVV + D   GE+P 
Sbjct: 427 IGFVDDDDEIFIVDRLKELIKYKGFQVAPAELEAMLITHPNMADAAVVSIKDDSCGELPV 486

Query: 547 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           A IV +  ++ TEDEI KY++  V  YK++  V F+E IPK+PSGK++R+ ++ ++
Sbjct: 487 AFIVRSNGSEITEDEIKKYISKQVVFYKRIHRVFFIEAIPKAPSGKILRKELRARL 537

[Close]

BLAST of Carg21220 vs. ExPASy TrEMBL
Match: A0A6J1G0B4 (4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=4 SV=1)

HSP 1 Score: 1168.7 bits (3022), Expect = 0.0e+00
Identity = 602/606 (99.34%), Postives = 603/606 (99.50%), Query Frame = 0


Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60

Query: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWH+LLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVM PNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAETAS 607
           RAE AS
Sbjct: 601 RAEAAS 606

[Close]

BLAST of Carg21220 vs. ExPASy TrEMBL
Match: A0A6J1HUV5 (4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 SV=1)

HSP 1 Score: 1158.3 bits (2995), Expect = 0.0e+00
Identity = 595/606 (98.18%), Postives = 600/606 (99.01%), Query Frame = 0


Query: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATC 60
           MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRR SMSAANNNS MATC
Sbjct: 1   MSGMNQQHPTKKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRSSMSAANNNSTMATC 60

Query: 61  IRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVR 120
           IRDS EDEEHIFRSQLPEVQVPDDITLPEFVLQNAE YADNVAFVEAVSGKAYTYREVVR
Sbjct: 61  IRDSAEDEEHIFRSQLPEVQVPDDITLPEFVLQNAELYADNVAFVEAVSGKAYTYREVVR 120

Query: 121 DTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180
           DTSRFAKALSSLRLKKGQ+VIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV
Sbjct: 121 DTSRFAKALSSLRLKKGQIVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQV 180

Query: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQ 240
           EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWH+LLEAADRAGNNFVKEDIKQ
Sbjct: 181 EVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHKLLEAADRAGNNFVKEDIKQ 240

Query: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGIT 300
           TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSG+PQEMVGKVTTLGLIPFFHIYGIT
Sbjct: 241 TDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGIPQEMVGKVTTLGLIPFFHIYGIT 300

Query: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 360
           GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLS L
Sbjct: 301 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSSL 360

Query: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 420
           KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYG+IGKDNLAAKKNTV
Sbjct: 361 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGNIGKDNLAAKKNTV 420

Query: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 480
           GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID+KGWMHTGD
Sbjct: 421 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNKGWMHTGD 480

Query: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540
           IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA
Sbjct: 481 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 540

Query: 541 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600
           ASIVM PNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI
Sbjct: 541 ASIVMAPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKI 600

Query: 601 RAETAS 607
           RAETAS
Sbjct: 601 RAETAS 606

[Close]

BLAST of Carg21220 vs. ExPASy TrEMBL
Match: A0A6J1DG05 (4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1027.3 bits (2655), Expect = 2.6e-296
Identity = 529/596 (88.76%), Postives = 559/596 (93.79%), Query Frame = 0


Query: 11  KKRLMPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEH 70
           +KR MPISP + L ISH QSM              PS     N SAM T IRD VEDEEH
Sbjct: 25  QKRPMPISPPITLHISHSQSM-----------KTEPSSMPVANISAMGTLIRDLVEDEEH 84

Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKAYTYREVVRDT RFAKALS
Sbjct: 85  IFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKAYTYREVVRDTKRFAKALS 144

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
           SLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVE ADAKLVVT
Sbjct: 145 SLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEAADAKLVVT 204

Query: 191 NSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSS 250
           NS +FEKVKELKLPVIV+GEELIEGSMNWH+LLEAADRAGNN VKEDIKQ+DLCALPFSS
Sbjct: 205 NSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNNHVKEDIKQSDLCALPFSS 264

Query: 251 GTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNK 310
           GTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIPFFHIYGITGICCATLRNK
Sbjct: 265 GTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIPFFHIYGITGICCATLRNK 324

Query: 311 GKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAA 370
           GKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAA
Sbjct: 325 GKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAA 384

Query: 371 PLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVK 430
           PLAPE+QT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+NL AKKNTVGRILPNLEVK
Sbjct: 385 PLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKENLTAKKNTVGRILPNLEVK 444

Query: 431 FIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDV 490
           FIDP+SGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID++GW+HTGDIGYIDD+GDV
Sbjct: 445 FIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDNRGWLHTGDIGYIDDNGDV 504

Query: 491 FIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAK 550
           FIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+V+ PN+K
Sbjct: 505 FIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVIAPNSK 564

Query: 551 ETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 607
           ETE+EII+YVASNVA+YKKVR+VHFV++IPKSPSGKVMRRLIKEKMI+KIRA+TAS
Sbjct: 565 ETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLIKEKMIDKIRAKTAS 609

[Close]

BLAST of Carg21220 vs. ExPASy TrEMBL
Match: A0A1S3BER3 (4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1)

HSP 1 Score: 1018.8 bits (2633), Expect = 9.1e-294
Identity = 518/592 (87.50%), Postives = 560/592 (94.59%), Query Frame = 0


Query: 15  MPISPSVALQISHPQSMIQAFSAPPLRRGRRPSMSAANNNSAMATCIRDSVEDEEHIFRS 74
           MPISPS  LQ+SH QSMIQ FS   L RG+   + A N  SAM T  +DSV+DE+HIFRS
Sbjct: 1   MPISPSRTLQVSHSQSMIQPFSTLLLSRGKLIHVEAENYYSAMTTRNQDSVQDEKHIFRS 60

Query: 75  QLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALSSLRL 134
           QLPEVQVP DITLPEFVLQNAESYA+NVAFVEA+SGKAYTYREV+RDT+RF+KAL SLRL
Sbjct: 61  QLPEVQVPCDITLPEFVLQNAESYAENVAFVEAMSGKAYTYREVLRDTNRFSKALHSLRL 120

Query: 135 KKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVTNSSS 194
           KKG VV+VVLPNVAEYAIVALGIM AGGVFSGVNPAAHISEIKKQVEVA+AKLVVTN+++
Sbjct: 121 KKGHVVLVVLPNVAEYAIVALGIMTAGGVFSGVNPAAHISEIKKQVEVAEAKLVVTNAAN 180

Query: 195 FEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCALPFSSGTTG 254
           FEKV+ELKLPVI++ EEL+EG+MNWH+LLEAADRAGNNFVKE+IKQTDLCALPFSSGTTG
Sbjct: 181 FEKVRELKLPVILLEEELMEGAMNWHKLLEAADRAGNNFVKEEIKQTDLCALPFSSGTTG 240

Query: 255 VSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCATLRNKGKVV 314
           VSKGVMLTHRNLVAN+CSTLSGVPQEM GKVTTLGLIPFFHIYGITGICC+TLRNKGKVV
Sbjct: 241 VSKGVMLTHRNLVANMCSTLSGVPQEMEGKVTTLGLIPFFHIYGITGICCSTLRNKGKVV 300

Query: 315 VMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAP 374
           VMGRFDLRTFINALIT E+TFAPIVPPIILALVKNPIV+EFDLS LKLQAIMTAAAPLAP
Sbjct: 301 VMGRFDLRTFINALITHEITFAPIVPPIILALVKNPIVEEFDLSSLKLQAIMTAAAPLAP 360

Query: 375 ELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDP 434
           ELQT+FE+KFPGVDVQEAYGLTEH CITLNYG+IGK+NL+AKKNTVGRILPNLEVKFIDP
Sbjct: 361 ELQTAFERKFPGVDVQEAYGLTEHCCITLNYGNIGKENLSAKKNTVGRILPNLEVKFIDP 420

Query: 435 DSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVD 494
           D+GRSLPKNTPGEICVRSQCVMQGYY NEEETSRTID KGWMHTGDIGYIDDDGDVFIVD
Sbjct: 421 DTGRSLPKNTPGEICVRSQCVMQGYYKNEEETSRTIDSKGWMHTGDIGYIDDDGDVFIVD 480

Query: 495 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVMTPNAKETED 554
           RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD+EAGEIPAAS+VM PN+ ETE+
Sbjct: 481 RIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDEEAGEIPAASVVMAPNSNETEE 540

Query: 555 EIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMIEKIRAETAS 607
           EIIK+VASNVAHYKKVRLVHFV+TIPKSPSGKVMRRLIKEKMIEKIRA+ ++
Sbjct: 541 EIIKHVASNVAHYKKVRLVHFVDTIPKSPSGKVMRRLIKEKMIEKIRADESA 592

[Close]

BLAST of Carg21220 vs. ExPASy TrEMBL
Match: A0A6J1DEE1 (4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111019748 PE=4 SV=1)

HSP 1 Score: 1010.0 bits (2610), Expect = 4.2e-291
Identity = 513/554 (92.60%), Postives = 541/554 (97.65%), Query Frame = 0


Query: 53  NNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKA 112
           N SAM T IRD VEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYA+NVAFVEAVSGKA
Sbjct: 5   NISAMGTLIRDLVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYAENVAFVEAVSGKA 64

Query: 113 YTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 172
           YTYREVVRDT RFAKALSSLRL+KGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH
Sbjct: 65  YTYREVVRDTKRFAKALSSLRLRKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAH 124

Query: 173 ISEIKKQVEVADAKLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNN 232
           ISEIKKQVE ADAKLVVTNS +FEKVKELKLPVIV+GEELIEGSMNWH+LLEAADRAGNN
Sbjct: 125 ISEIKKQVEAADAKLVVTNSPNFEKVKELKLPVIVLGEELIEGSMNWHKLLEAADRAGNN 184

Query: 233 FVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIP 292
            VKEDIKQ+DLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVP+E  GKVTTLGLIP
Sbjct: 185 HVKEDIKQSDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPRETEGKVTTLGLIP 244

Query: 293 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 352
           FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV
Sbjct: 245 FFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIV 304

Query: 353 KEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDN 412
           +EFDLS LKLQAIMTAAAPLAPE+QT+FEKKFPGVDVQEAYGLTEHSCITLNYGSIGK+N
Sbjct: 305 EEFDLSSLKLQAIMTAAAPLAPEIQTAFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKEN 364

Query: 413 LAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDD 472
           L AKKNTVGRILPNLEVKFIDP+SGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTID+
Sbjct: 365 LTAKKNTVGRILPNLEVKFIDPESGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDN 424

Query: 473 KGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD 532
           +GW+HTGDIGYIDD+GDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD
Sbjct: 425 RGWLHTGDIGYIDDNGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPD 484

Query: 533 KEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLI 592
           +EAGEIPAAS+V+ PN+KETE+EII+YVASNVA+YKKVR+VHFV++IPKSPSGKVMRRLI
Sbjct: 485 EEAGEIPAASVVIAPNSKETEEEIIQYVASNVAYYKKVRVVHFVDSIPKSPSGKVMRRLI 544

Query: 593 KEKMIEKIRAETAS 607
           KEKMI+KIRA+TAS
Sbjct: 545 KEKMIDKIRAKTAS 558

[Close]

BLAST of Carg21220 vs. TAIR 10
Match: AT1G62940.1 (acyl-CoA synthetase 5 )

HSP 1 Score: 788.9 bits (2036), Expect = 2.9e-228
Identity = 389/532 (73.12%), Postives = 459/532 (86.28%), Query Frame = 0


Query: 66  EDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRF 125
           ED E+IFRS  P V +PD +TLPEFVLQ  E Y +NVAFVEAV+GKA TY +VVRDT R 
Sbjct: 7   EDNEYIFRSLYPSVPIPDKLTLPEFVLQGVEEYTENVAFVEAVTGKAVTYGDVVRDTKRL 66

Query: 126 AKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADA 185
           AKAL+SL L+KGQV++VVLPNVAEY I+ALGIM+AGGVFSG NP A +SEIKKQVE + A
Sbjct: 67  AKALTSLGLRKGQVMVVVLPNVAEYGIIALGIMSAGGVFSGANPTALVSEIKKQVEASGA 126

Query: 186 KLVVTNSSSFEKVKELKLPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCA 245
           + ++T+++++EKVK L LPVIV+GEE IEG++NW  LLEA D+ G+    E+I QTDLCA
Sbjct: 127 RGIITDATNYEKVKSLGLPVIVLGEEKIEGAVNWKDLLEAGDKCGDT-DNEEILQTDLCA 186

Query: 246 LPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMVGKVTTLGLIPFFHIYGITGICCA 305
           LPFSSGTTG+ KGVMLTHRNL+ANLCSTL GV  EM+G++ TLGLIPFFHIYGI GICCA
Sbjct: 187 LPFSSGTTGLQKGVMLTHRNLIANLCSTLFGVRSEMIGQIVTLGLIPFFHIYGIVGICCA 246

Query: 306 TLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQAI 365
           T++NKGKVV M R+DLR F+NALI  EV+FAPIVPPIIL LVKNPIV EFDLS LKLQ++
Sbjct: 247 TMKNKGKVVAMSRYDLRIFLNALIAHEVSFAPIVPPIILNLVKNPIVDEFDLSKLKLQSV 306

Query: 366 MTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRILP 425
           MTAAAPLAPEL T+FE KFP V VQEAYGLTEHSCITL +G   K    AK+N+VG ILP
Sbjct: 307 MTAAAPLAPELLTAFEAKFPNVQVQEAYGLTEHSCITLTHGDPEKGQGIAKRNSVGFILP 366

Query: 426 NLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYID 485
           NLEVKFIDPD+GRSLPKNT GE+CVRSQCVMQGY+ N+EET +TID++GW+HTGDIGYID
Sbjct: 367 NLEVKFIDPDTGRSLPKNTSGELCVRSQCVMQGYFMNKEETDKTIDEQGWLHTGDIGYID 426

Query: 486 DDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIVM 545
           DDGD+FIVDRIKELIKYKGFQVAPAELEAILLTHPS+ED AVVPLPD+EAGEIPAA +V+
Sbjct: 427 DDGDIFIVDRIKELIKYKGFQVAPAELEAILLTHPSVEDVAVVPLPDEEAGEIPAACVVI 486

Query: 546 TPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKMI 598
            P A E E++I+ +VA+NVAHYKKVR VHFV++IPKS SGK+MRRL+++K++
Sbjct: 487 NPKATEKEEDILNFVAANVAHYKKVRAVHFVDSIPKSLSGKIMRRLLRDKIL 537

[Close]

BLAST of Carg21220 vs. TAIR 10
Match: AT3G21240.1 (4-coumarate:CoA ligase 2 )

HSP 1 Score: 394.4 bits (1012), Expect = 1.6e-109
Identity = 200/532 (37.59%), Postives = 328/532 (61.65%), Query Frame = 0


Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRS+LP++ +P+ + L +++ +N   +A     +   +G+ YTY +V   + + A  L 
Sbjct: 22  IFRSRLPDIYIPNHLPLHDYIFENISEFAAKPCLINGPTGEVYTYADVHVTSRKLAAGLH 81

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
           +L +K+  VV+++LPN  E  +  L     G + +  NP    +EI KQ + + AKL+VT
Sbjct: 82  NLGVKQHDVVMILLPNSPEVVLTFLAASFIGAITTSANPFFTPAEISKQAKASAAKLIVT 141

Query: 191 NSSSFEKVKELK----LPVIVVGEELIEGSMNWHQLLEAADRAGNNFVKEDIKQTDLCAL 250
            S   +K+K L+    L V    + + E  + + +L ++ +   ++ + E I   D+ AL
Sbjct: 142 QSRYVDKIKNLQNDGVLIVTTDSDAIPENCLRFSELTQSEEPRVDS-IPEKISPEDVVAL 201

Query: 251 PFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGITGICC 310
           PFSSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY +  I  
Sbjct: 202 PFSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFNRDDVILCVLPMFHIYALNSIML 261

Query: 311 ATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGLKLQA 370
            +LR    +++M +F++   +  +   +VT A +VPPI+LA+ K+P  +++DLS +++  
Sbjct: 262 CSLRVGATILIMPKFEITLLLEQIQRCKVTVAMVVPPIVLAIAKSPETEKYDLSSVRM-- 321

Query: 371 IMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTVGRIL 430
           + + AAPL  EL+ +   KFP   + + YG+TE   +        K+    K    G ++
Sbjct: 322 VKSGAAPLGKELEDAISAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGACGTVV 381

Query: 431 PNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGDIGYI 490
            N E+K +DPD+G SLP+N PGEIC+R   +M+GY N+   T+ TID  GW+HTGD+G+I
Sbjct: 382 RNAEMKILDPDTGDSLPRNKPGEICIRGNQIMKGYLNDPLATASTIDKDGWLHTGDVGFI 441

Query: 491 DDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPAASIV 550
           DDD ++FIVDR+KELIKYKGFQVAPAELE++L+ HP I D AVV + +++AGE+P A +V
Sbjct: 442 DDDDELFIVDRLKELIKYKGFQVAPAELESLLIGHPEINDVAVVAMKEEDAGEVPVAFVV 501

Query: 551 MTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
            + ++  +EDEI ++V+  V  YK++  V F ++IPK+PSGK++R+ ++ ++
Sbjct: 502 RSKDSNISEDEIKQFVSKQVVFYKRINKVFFTDSIPKAPSGKILRKDLRARL 550

[Close]

BLAST of Carg21220 vs. TAIR 10
Match: AT1G51680.1 (4-coumarate:CoA ligase 1 )

HSP 1 Score: 386.3 bits (991), Expect = 4.4e-107
Identity = 196/536 (36.57%), Postives = 322/536 (60.07%), Query Frame = 0


Query: 71  IFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFVEAVSGKAYTYREVVRDTSRFAKALS 130
           IFRS+LP++ +P+ ++L +++ QN   +A     +   +G  YTY +V   + + A    
Sbjct: 24  IFRSKLPDIYIPNHLSLHDYIFQNISEFATKPCLINGPTGHVYTYSDVHVISRQIAANFH 83

Query: 131 SLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEIKKQVEVADAKLVVT 190
            L + +  VV+++LPN  E+ +  L     G   +  NP    +EI KQ + ++ KL++T
Sbjct: 84  KLGVNQNDVVMLLLPNCPEFVLSFLAASFRGATATAANPFFTPAEIAKQAKASNTKLIIT 143

Query: 191 NSSSFEKVKELK----LPVIVVGEE----LIEGSMNWHQLLEAADRAGNNFVKEDIKQTD 250
            +   +K+K L+    + ++ + +     + EG + + +L ++   A       +I   D
Sbjct: 144 EARYVDKIKPLQNDDGVVIVCIDDNESVPIPEGCLRFTELTQSTTEASEVIDSVEISPDD 203

Query: 251 LCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV--GKVTTLGLIPFFHIYGIT 310
           + ALP+SSGTTG+ KGVMLTH+ LV ++   + G    +        L ++P FHIY + 
Sbjct: 204 VVALPYSSGTTGLPKGVMLTHKGLVTSVAQQVDGENPNLYFHSDDVILCVLPMFHIYALN 263

Query: 311 GICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPIILALVKNPIVKEFDLSGL 370
            I    LR    +++M +F++   +  +   +VT AP+VPPI+LA+ K+   +++DLS +
Sbjct: 264 SIMLCGLRVGAAILIMPKFEINLLLELIQRCKVTVAPMVPPIVLAIAKSSETEKYDLSSI 323

Query: 371 KLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITLNYGSIGKDNLAAKKNTV 430
           ++  + + AAPL  EL+ +   KFP   + + YG+TE   +        K+    K    
Sbjct: 324 RV--VKSGAAPLGKELEDAVNAKFPNAKLGQGYGMTEAGPVLAMSLGFAKEPFPVKSGAC 383

Query: 431 GRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNEEETSRTIDDKGWMHTGD 490
           G ++ N E+K +DPD+G SL +N PGEIC+R   +M+GY NN   T+ TID  GW+HTGD
Sbjct: 384 GTVVRNAEMKIVDPDTGDSLSRNQPGEICIRGHQIMKGYLNNPAATAETIDKDGWLHTGD 443

Query: 491 IGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIEDAAVVPLPDKEAGEIPA 550
           IG IDDD ++FIVDR+KELIKYKGFQVAPAELEA+L+ HP I D AVV + ++ AGE+P 
Sbjct: 444 IGLIDDDDELFIVDRLKELIKYKGFQVAPAELEALLIGHPDITDVAVVAMKEEAAGEVPV 503

Query: 551 ASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSPSGKVMRRLIKEKM 597
           A +V + +++ +ED++ ++V+  V  YK++  V F E+IPK+PSGK++R+ ++ K+
Sbjct: 504 AFVVKSKDSELSEDDVKQFVSKQVVFYKRINKVFFTESIPKAPSGKILRKDLRAKL 557

[Close]

BLAST of Carg21220 vs. TAIR 10
Match: AT1G65060.1 (4-coumarate:CoA ligase 3 )

HSP 1 Score: 382.9 bits (982), Expect = 4.9e-106
Identity = 208/556 (37.41%), Postives = 329/556 (59.17%), Query Frame = 0


Query: 46  PSMSAANNNSAMATCIRDSVEDEEHIFRSQLPEVQVPDDITLPEFVLQNAESYADNVAFV 105
           P +    + S ++  +         IFRS+LP++ +P+ + L  +  +   S +D    +
Sbjct: 9   PQIHKPTDTSVVSDDVLPHSPPTPRIFRSKLPDIDIPNHLPLHTYCFEKLSSVSDKPCLI 68

Query: 106 EAVSGKAYTYREVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFS 165
              +GK+YTY E      R A  L  L ++KG V++++L N AE+    +G    G V +
Sbjct: 69  VGSTGKSYTYGETHLICRRVASGLYKLGIRKGDVIMILLQNSAEFVFSFMGASMIGAVST 128

Query: 166 GVNPAAHISEIKKQVEVADAKLVVTNSSSFEKVKEL--KLPVIVVGEELIEGSMNWHQLL 225
             NP     E+ KQ++ + AKL++T+S   +K+K L   L +I   E   E  + +  L+
Sbjct: 129 TANPFYTSQELYKQLKSSGAKLIITHSQYVDKLKNLGENLTLITTDEPTPENCLPFSTLI 188

Query: 226 EAADRAGNNFVKE-DIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEMV 285
              D   N F +  DI   D  ALPFSSGTTG+ KGV+LTH++L+ ++   + G    + 
Sbjct: 189 --TDDETNPFQETVDIGGDDAAALPFSSGTTGLPKGVVLTHKSLITSVAQQVDGDNPNLY 248

Query: 286 GKV--TTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVP 345
            K     L ++P FHIY +  +   +LR+   V++M +F++   ++ +    VT A +VP
Sbjct: 249 LKSNDVILCVLPLFHIYSLNSVLLNSLRSGATVLLMHKFEIGALLDLIQRHRVTIAALVP 308

Query: 346 PIILALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSC 405
           P+++AL KNP V  +DLS ++   +++ AAPL  ELQ S  ++ P   + + YG+TE   
Sbjct: 309 PLVIALAKNPTVNSYDLSSVRF--VLSGAAPLGKELQDSLRRRLPQAILGQGYGMTEAGP 368

Query: 406 ITLNYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYY 465
           +        K+ +  K  + G ++ N E+K +  ++  SL  N PGEIC+R Q +M+ Y 
Sbjct: 369 VLSMSLGFAKEPIPTKSGSCGTVVRNAELKVVHLETRLSLGYNQPGEICIRGQQIMKEYL 428

Query: 466 NNEEETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHP 525
           N+ E TS TID++GW+HTGDIGY+D+D ++FIVDR+KE+IK+KGFQV PAELE++L+ H 
Sbjct: 429 NDPEATSATIDEEGWLHTGDIGYVDEDDEIFIVDRLKEVIKFKGFQVPPAELESLLINHH 488

Query: 526 SIEDAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIP 585
           SI DAAVVP  D+ AGE+P A +V +     TE+++ +YVA  V  YK++  V FV +IP
Sbjct: 489 SIADAAVVPQNDEVAGEVPVAFVVRSNGNDITEEDVKEYVAKQVVFYKRLHKVFFVASIP 548

Query: 586 KSPSGKVMRRLIKEKM 597
           KSPSGK++R+ +K K+
Sbjct: 549 KSPSGKILRKDLKAKL 560

[Close]

BLAST of Carg21220 vs. TAIR 10
Match: AT3G21230.1 (4-coumarate:CoA ligase 5 )

HSP 1 Score: 377.5 bits (968), Expect = 2.0e-104
Identity = 196/553 (35.44%), Postives = 331/553 (59.86%), Query Frame = 0


Query: 63  DSVEDEEH--IFRSQLPEVQVPDDITLPEFVLQ----NAESYADNVAFVEAVSGKAYTYR 122
           D  E+  H  IFRS+LP++ +P+ + L ++V Q    + +  +     ++  +G+  TY 
Sbjct: 18  DEEEEPSHDFIFRSKLPDIFIPNHLPLTDYVFQRFSGDGDGDSSTTCIIDGATGRILTYA 77

Query: 123 EVVRDTSRFAKALSSLRLKKGQVVIVVLPNVAEYAIVALGIMAAGGVFSGVNPAAHISEI 182
           +V  +  R A  +  L ++ G VV+++LPN  E+A+  L +   G V +  NP     EI
Sbjct: 78  DVQTNMRRIAAGIHRLGIRHGDVVMLLLPNSPEFALSFLAVAYLGAVSTTANPFYTQPEI 137

Query: 183 KKQVEVADAKLVVTNSSSFEKVKELKLPVIVV------GEELI-----EGSMNWHQLLEA 242
            KQ + + AK+++T     +K+  LK   +++      G+  +     +G +++ +L +A
Sbjct: 138 AKQAKASAAKMIITKKCLVDKLTNLKNDGVLIVCLDDDGDNGVVSSSDDGCVSFTELTQA 197

Query: 243 ADRAGNNFVKEDIKQTDLCALPFSSGTTGVSKGVMLTHRNLVANLCSTLSGVPQEM--VG 302
            +      +K  I   D  A+P+SSGTTG+ KGVM+TH+ LV ++   + G    +    
Sbjct: 198 DE---TELLKPKISPEDTVAMPYSSGTTGLPKGVMITHKGLVTSIAQKVDGENPNLNFTA 257

Query: 303 KVTTLGLIPFFHIYGITGICCATLRNKGKVVVMGRFDLRTFINALITQEVTFAPIVPPII 362
               L  +P FHIY +  +  + +R    ++++ RF+L   +  +   +VT  P+ PP++
Sbjct: 258 NDVILCFLPMFHIYALDALMLSAMRTGAALLIVPRFELNLVMELIQRYKVTVVPVAPPVV 317

Query: 363 LALVKNPIVKEFDLSGLKLQAIMTAAAPLAPELQTSFEKKFPGVDVQEAYGLTEHSCITL 422
           LA +K+P  + +DLS +++  +++ AA L  EL+ +   KFP     + YG+TE   +  
Sbjct: 318 LAFIKSPETERYDLSSVRI--MLSGAATLKKELEDAVRLKFPNAIFGQGYGMTESGTVAK 377

Query: 423 NYGSIGKDNLAAKKNTVGRILPNLEVKFIDPDSGRSLPKNTPGEICVRSQCVMQGYYNNE 482
           +  +  K+    K    G ++ N E+K +D ++G SLP+N  GEICVR   +M+GY N+ 
Sbjct: 378 SL-AFAKNPFKTKSGACGTVIRNAEMKVVDTETGISLPRNKSGEICVRGHQLMKGYLNDP 437

Query: 483 EETSRTIDDKGWMHTGDIGYIDDDGDVFIVDRIKELIKYKGFQVAPAELEAILLTHPSIE 542
           E T+RTID  GW+HTGDIG++DDD ++FIVDR+KELIK+KG+QVAPAELEA+L++HPSI+
Sbjct: 438 EATARTIDKDGWLHTGDIGFVDDDDEIFIVDRLKELIKFKGYQVAPAELEALLISHPSID 497

Query: 543 DAAVVPLPDKEAGEIPAASIVMTPNAKETEDEIIKYVASNVAHYKKVRLVHFVETIPKSP 597
           DAAVV + D+ A E+P A +  +  ++ TED++  YV   V HYK++++V F+E IPK+ 
Sbjct: 498 DAAVVAMKDEVADEVPVAFVARSQGSQLTEDDVKSYVNKQVVHYKRIKMVFFIEVIPKAV 557

The following BLAST results are available for this feature:

BLAST of Carg21220 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012722.1	0.0e+00	100.00	4-coumarate--CoA ligase-like 1 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022945246.1	0.0e+00	99.34	4-coumarate--CoA ligase-like 1 [Cucurbita moschata]	[more]
XP_023541569.1	0.0e+00	99.01	4-coumarate--CoA ligase-like 1 [Cucurbita pepo subsp. pepo]	[more]
XP_022966799.1	0.0e+00	98.18	4-coumarate--CoA ligase-like 1 [Cucurbita maxima]	[more]
KAG6573594.1	2.3e-307	96.18	4-coumarate--CoA ligase-like 1, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg21220 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LQ12	4.1e-227	73.12	4-coumarate--CoA ligase-like 1 OS=Arabidopsis thaliana OX=3702 GN=4CLL1 PE=1 SV=...	[more]
Q7XXL2	8.7e-201	64.38	4-coumarate--CoA ligase-like 9 OS=Oryza sativa subsp. japonica OX=39947 GN=4CLL9...	[more]
P14912	1.5e-112	38.95	4-coumarate--CoA ligase 1 OS=Petroselinum crispum OX=4043 GN=4CL1 PE=2 SV=1	[more]
P31684	1.5e-112	38.72	4-coumarate--CoA ligase 1 OS=Solanum tuberosum OX=4113 GN=4CL1 PE=3 SV=1	[more]
A0A2H5AIX5	2.6e-112	39.93	4-coumarate-CoA ligase 1 OS=Narcissus pseudonarcissus OX=39639 GN=4CL1 PE=2 SV=1	[more]

BLAST of Carg21220 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G0B4	0.0e+00	99.34	4-coumarate--CoA ligase-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111449548 PE=...	[more]
A0A6J1HUV5	0.0e+00	98.18	4-coumarate--CoA ligase-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111466396 PE=4 ...	[more]
A0A6J1DG05	2.6e-296	88.76	4-coumarate--CoA ligase-like 1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC1...	[more]
A0A1S3BER3	9.1e-294	87.50	4-coumarate--CoA ligase-like 1 OS=Cucumis melo OX=3656 GN=LOC103488874 PE=4 SV=1	[more]
A0A6J1DEE1	4.2e-291	92.60	4-coumarate--CoA ligase-like 1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC1...	[more]

BLAST of Carg21220 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G62940.1	2.9e-228	73.12	acyl-CoA synthetase 5	[more]
AT3G21240.1	1.6e-109	37.59	4-coumarate:CoA ligase 2	[more]
AT1G51680.1	4.4e-107	36.57	4-coumarate:CoA ligase 1	[more]
AT1G65060.1	4.9e-106	37.41	4-coumarate:CoA ligase 3	[more]
AT3G21230.1	2.0e-104	35.44	4-coumarate:CoA ligase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000873	AMP-dependent synthetase/ligase	PFAM	PF00501	AMP-binding	coord: 93..502 e-value: 3.2E-96 score: 322.5
IPR025110	AMP-binding enzyme, C-terminal domain	PFAM	PF13193	AMP-binding_C	coord: 511..586 e-value: 3.7E-16 score: 59.8
IPR042099	ANL, N-terminal domain	GENE3D	3.40.50.12780		coord: 69..497 e-value: 6.0E-131 score: 439.1
None	No IPR available	GENE3D	3.30.300.30		coord: 498..600 e-value: 1.5E-30 score: 107.9
None	No IPR available	PANTHER	PTHR24096:SF389	4-COUMARATE--COA LIGASE-LIKE 1	coord: 63..599
None	No IPR available	PANTHER	PTHR24096	LONG-CHAIN-FATTY-ACID--COA LIGASE	coord: 63..599
None	No IPR available	CDD	cd05904	4CL	coord: 81..590 e-value: 0.0 score: 667.016
None	No IPR available	SUPERFAMILY	56801	Acetyl-CoA synthetase-like	coord: 66..599
IPR020845	AMP-binding, conserved site	PROSITE	PS00455	AMP_BINDING	coord: 246..257

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21220-RA	Carg21220-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0046949	fatty-acyl-CoA biosynthetic process
biological_process	GO:0001676	long-chain fatty acid metabolic process
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004467	long-chain fatty acid-CoA ligase activity