Carg21200 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21200
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectin lyase-like superfamily protein
Location	Carg_Chr18: 7230931 .. 7234734 (+)
RNA-Seq Expression	Carg21200
Synteny	Carg21200

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTCTTTTTCAAATATATTTCGTATTCCGTTCCATACTTGCTTTGAGCCACTCTCATCACACTTGAAGTGATCAGATTCGGAAGAACATTCAACCATATCTCACTTTTTTTTTCTTTTTCTTTTTTGGTTTTCGATCCGTTTGAGTTTTCCGGTGGTTCATCTCCGCCTTGGTTTCCCGTCGGAAGAGAAGGGAGAAGAAGCAGTTGAATGCGGTGAAGCAACAGGCAGGGCAGGACCTACTGCTTAATATTATCCAGTAGCAACGGTCAGCGTCAATGATTTGTGTGGGTGGTGGTGCCTCAAGCCTCATTATGATCCACCTAATCACATTTATAACTGCTGGATCTTGATCTTCCTCAACAAGCTCCCCTCATTTCATTTTCTTTTTCATCTGAAAAGAGATTTGGACGTTTGGAGACCAGGAGCCCGGCCGGTGTTTTGATTTGTCATCCATCTGATCAACCGGAGCAACTCAGTCGTATCGTATGAAGACACCTCTCACTGTGAGTTGTTTCCTCTTTCTCAACTCCAACACTTGTAAAATTCACACCAATTCCTACTAATTTCTTCAATTTGATGGTCTGAGGTTTTGCTTTTGAGCTACATTTTGGCTAAACCCCAAAATCCCGATTAATGTTGAATGCCTAGCGCAATTCTCTCTCCGTTGTGGCTGTTGCTACCTTAATCTTAGGTTTTCTTACATTATTCGTAAAATTGGGGCTAGTGGACGGCAAATTTTCTGATTCCCGTTTTCCTATTATGATTTATTTAATTGAAGTTCTGCAAGCTCAAACCAATTACTTTTACTTGATCGCCTCACTTCGATCTATCTTGATTGATATTCAGCTGTTTTTCTTTGCACCTTTGTAGAAGAAGATCCCTCTGGTAACTTTGTTCCTTGTTCATTGTTAGAGCAGGAAGAACAATGTTAATTGCGGAACTAAGCCAGATGATGCACTTGATCCCTTCCCTTTTGATACTTTTAACAATAGTGGCTTTATTCTTTCAGCTGGTGGATGTTCTTCTGTTGCTTGCATTGTTTTGTATTTCTCCATGGGTTGCTGGGAGTAGCAGCTCTTATTGCAAGCTGACTAATACAGGAGAAATTCGACCCCACAGTGTCTCTATCACTGATTTTGGGGCAGTTGGAGATGGTGTCACTCTCAACACAAAAGCATTCCAAAATGCTATTTTCTACCTCAACTCTTTTGCCGACAAGGGTGGTGCCAAGCTTTTTGTCCCAGCTGGGCGTTGGTTGACAGGAAGCTTCGATCTTATCAGTCATTTAACTTTGTGGTTAGATAAGGATGCTGTGATTCTTGGCTCAACGGTAAGCTCTTGCTTCTTGGTTGAATAAAAGTGTCTACCAAGCAAAGTTTTGAGTTAACTGGTTGTTAGTACACCAAAATTCTCCCCTTGCAAGTGCTTTTTTAATGAATTTGAGCTGATTTTCAATCAGATTAAAGTACAAGATGTAGTGGCATTGTATTGTTCATCAAAATTCTCCCCTTGAAGGTGCATTTTTAATGGATTTGAGCTGATTTTCAATCAGATTGAAGTACAAGATGTTGTGGCATTGTATTGTACACCAAAATTCTCCCCTTGAAAGTGCATTTTTAATGAATTTGAGCTGATTTTTAATCAGATTAAAGTACAAAATGTAGTGGCATTGTATTGTCTAATGATGAATGTTTTGTAATTTTTTCAGATCTCTGATGACTGGCCGATTATCAAACCACTGCCATCATATGGTAGAGGACGCGAGTTGCCAGGTGAACGGCATCGTAGCCTCATCTATGGATGCAATTTGACGAATGTTGTCATCACAGGTAGCCCATATTGAAGAACAGTTGTATTCTGCAGTAACCTTTATACTCCTTAATTGTTGTTACTTTTGGTTATGTGCAGGTGAAAATGGAACCATTGATGGACAGGGTAGCATCTGGTGGGACTGGTTCCATAGTAAAACGTTAAACTATACGAGGCCGCATTTAGTTGAAATCATGAATTCAACTAATGTTGTAATCTCAAATCTGACATTCAAGAATTCACCCTTTTGGACCCTCCATCCTGTATATTGCAGGTCTTTCCCCATATGGGGAAGCATAATGTTCATTCTTTCCTTGATCGTTGAACTCGATACTTTATCTATTACGAAGACATGAAACTGAGCTCTTCATTTTTCTATTTTGAATAAACAGCAAAGTCCTTGTTCAGAATGTGACAATCCTTGCACCATCTGATTCTCCAAACACCGATGGTATTGATCCAGGTGAGGCATCATGTTCTTGATACTAACCTTTTATAATCAGTTTATTTATTTAGAGCATTCTTTAGTTCAAAAGTATGACTCTGCAAATATGTAGTGAATAGAAAAAAGGTTTCATATATATATATAATATTCGTGAGATCCCACATCGGTTGAGAGGAGAACGAAGCATTCTTTATAAGGATGTTGAAACCTCTCCCTAGAAAACGCGTTTTAAAAACCCTGAGGGGAAGCCCGAAAGGGAAAAGCCCAAAGAGGACAATATTTGCTAGCGGTGGCCTTGGGTCGTTAGAATATTGCTTGAGCCTTGAAGAATAAGGTAATTCTCAGAAAGCTAAAATTTTGAATATGATGTGCTTCAAGTTAATTATAGTCAGTGCCCCTGCCTTTTAACCGTCTGTTTGAAGCTGACATTGTTATCTTACACAAACTTCTCATCTCCAGATTCTTCATCTGAAGTTTGCATTGAAGATTGCTACATTAGCACAGGCGATGATCTAATTTCGATCAAGAGTGGGTGGGATGAATATGGTGTTGAGTATGCTCGTCCTAGTACGAACATTACCATTCGAAGGCTCACTGGGCAAACTACTAGTGCGGGAATTGCAATAGGAAGTGAGATGTCTGGAGGCGTGTCAGAAATTCTTGTAGAAAACATCCTATTTTTCAACTCAAACATGGGTATCAGAATTAAGACGTCTCCAGGAAGGGGTGGTTACGTGCGGAATGTTTACATATCGAATGTGAGCTTGATCGATGTAAACGTAGCTATAAGGTTCACTGGTAAGTATGGTGAGCACCCAGATTCTGATTATAATCCAAATGCTCTTCCGACAGTTGAAAGGATCACAATTAAACATGTTAGTGGAGAGAATATTAAAACAGCAGGCGTTTTAGAGGGCATAGAAGGAGATGATTTTCATAATATTTGCTTAGCCAATATCACGCTTAATGTGAGCTCAATGCACAAATGGAACTGTTCGCATGTGCAAGGATTTTCTGACTTTGTTTCTCCAAAGACATGCCATTCTCTGAGGGAAAGAATTTTTCCCCAACATATTTCGGATTGTTATCAGCCACCAAATTTCATTGCTCAGGTATATTCTCTGAGCTTTGTTGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGGTTTAGGCTTTGTTGTCATCATTCTTTCAATCGTTTTCGAATATTCGAGTGATTGTATGATTTTGTCTAGTCTCTTGTGTGATAGATGAGTTCAACATGGACCGACTTCATCGGGATGTCGATTGCTTTGCCTGATGAATCAAGTAAAAGCTAAGAGCTGTGTGGCTGAACACTTTCAAATGGCAGGTACACAAACCAAATGTTATTTGTTTTCTGGACTTGGTGTGCAGCAAAGTTAATAATGGAGTGATTAATTAGAATATGTATATAGAGAGAGTTTCTTTTCACACACATTTGCAACCACACTTAGAATACACAAAAGTTAGTGTGGGTGTAATGTATGAACTGAGTTTCTTAAATGATAGCTGAGCAAGGTTTCTTTGGG

mRNA sequence

AATTCTTTTTCAAATATATTTCGTATTCCGTTCCATACTTGCTTTGAGCCACTCTCATCACACTTGAAGTGATCAGATTCGGAAGAACATTCAACCATATCTCACTTTTTTTTTCTTTTTCTTTTTTGGTTTTCGATCCGTTTGAGTTTTCCGGTGGTTCATCTCCGCCTTGGTTTCCCGTCGGAAGAGAAGGGAGAAGAAGCAGTTGAATGCGGTGAAGCAACAGGCAGGGCAGGACCTACTGCTTAATATTATCCAGTAGCAACGGTCAGCGTCAATGATTTGTGTGGGTGGTGGTGCCTCAAGCCTCATTATGATCCACCTAATCACATTTATAACTGCTGGATCTTGATCTTCCTCAACAAGCTCCCCTCATTTCATTTTCTTTTTCATCTGAAAAGAGATTTGGACGTTTGGAGACCAGGAGCCCGGCCGGTGTTTTGATTTGTCATCCATCTGATCAACCGGAGCAACTCAGTCGTATCGTATGAAGACACCTCTCACTGAAGAACAATGTTAATTGCGGAACTAAGCCAGATGATGCACTTGATCCCTTCCCTTTTGATACTTTTAACAATAGTGGCTTTATTCTTTCAGCTGGTGGATGTTCTTCTGTTGCTTGCATTGTTTTGTATTTCTCCATGGGTTGCTGGGAGTAGCAGCTCTTATTGCAAGCTGACTAATACAGGAGAAATTCGACCCCACAGTGTCTCTATCACTGATTTTGGGGCAGTTGGAGATGGTGTCACTCTCAACACAAAAGCATTCCAAAATGCTATTTTCTACCTCAACTCTTTTGCCGACAAGGGTGGTGCCAAGCTTTTTGTCCCAGCTGGGCGTTGGTTGACAGGAAGCTTCGATCTTATCAGTCATTTAACTTTGTGGTTAGATAAGGATGCTGTGATTCTTGGCTCAACGATCTCTGATGACTGGCCGATTATCAAACCACTGCCATCATATGGTAGAGGACGCGAGTTGCCAGGTGAACGGCATCGTAGCCTCATCTATGGATGCAATTTGACGAATGTTGTCATCACAGGTGAAAATGGAACCATTGATGGACAGGGTAGCATCTGGTGGGACTGGTTCCATAGTAAAACGTTAAACTATACGAGGCCGCATTTAGTTGAAATCATGAATTCAACTAATGTTGTAATCTCAAATCTGACATTCAAGAATTCACCCTTTTGGACCCTCCATCCTGTATATTGCAGCAAAGTCCTTGTTCAGAATGTGACAATCCTTGCACCATCTGATTCTCCAAACACCGATGGTATTGATCCAGATTCTTCATCTGAAGTTTGCATTGAAGATTGCTACATTAGCACAGGCGATGATCTAATTTCGATCAAGAGTGGGTGGGATGAATATGGTGTTGAGTATGCTCGTCCTAGTACGAACATTACCATTCGAAGGCTCACTGGGCAAACTACTAGTGCGGGAATTGCAATAGGAAGTGAGATGTCTGGAGGCGTGTCAGAAATTCTTGTAGAAAACATCCTATTTTTCAACTCAAACATGGGTATCAGAATTAAGACGTCTCCAGGAAGGGGTGGTTACGTGCGGAATGTTTACATATCGAATGTGAGCTTGATCGATGTAAACGTAGCTATAAGGTTCACTGGTAAGTATGGTGAGCACCCAGATTCTGATTATAATCCAAATGCTCTTCCGACAGTTGAAAGGATCACAATTAAACATGTTAGTGGAGAGAATATTAAAACAGCAGGCGTTTTAGAGGGCATAGAAGGAGATGATTTTCATAATATTTGCTTAGCCAATATCACGCTTAATGTGAGCTCAATGCACAAATGGAACTGTTCGCATGTGCAAGGATTTTCTGACTTTGTTTCTCCAAAGACATGCCATTCTCTGAGGGAAAGAATTTTTCCCCAACATATTTCGGATTGTTATCAGCCACCAAATTTCATTGCTCAGTCTCTTGTGTGATAGATGAGTTCAACATGGACCGACTTCATCGGGATGTCGATTGCTTTGCCTGATGAATCAAGTAAAAGCTAAGAGCTGTGTGGCTGAACACTTTCAAATGGCAGGTACACAAACCAAATGTTATTTGTTTTCTGGACTTGGTGTGCAGCAAAGTTAATAATGGAGTGATTAATTAGAATATGTATATAGAGAGAGTTTCTTTTCACACACATTTGCAACCACACTTAGAATACACAAAAGTTAGTGTGGGTGTAATGTATGAACTGAGTTTCTTAAATGATAGCTGAGCAAGGTTTCTTTGGG

Coding sequence (CDS)

ATGTTAATTGCGGAACTAAGCCAGATGATGCACTTGATCCCTTCCCTTTTGATACTTTTAACAATAGTGGCTTTATTCTTTCAGCTGGTGGATGTTCTTCTGTTGCTTGCATTGTTTTGTATTTCTCCATGGGTTGCTGGGAGTAGCAGCTCTTATTGCAAGCTGACTAATACAGGAGAAATTCGACCCCACAGTGTCTCTATCACTGATTTTGGGGCAGTTGGAGATGGTGTCACTCTCAACACAAAAGCATTCCAAAATGCTATTTTCTACCTCAACTCTTTTGCCGACAAGGGTGGTGCCAAGCTTTTTGTCCCAGCTGGGCGTTGGTTGACAGGAAGCTTCGATCTTATCAGTCATTTAACTTTGTGGTTAGATAAGGATGCTGTGATTCTTGGCTCAACGATCTCTGATGACTGGCCGATTATCAAACCACTGCCATCATATGGTAGAGGACGCGAGTTGCCAGGTGAACGGCATCGTAGCCTCATCTATGGATGCAATTTGACGAATGTTGTCATCACAGGTGAAAATGGAACCATTGATGGACAGGGTAGCATCTGGTGGGACTGGTTCCATAGTAAAACGTTAAACTATACGAGGCCGCATTTAGTTGAAATCATGAATTCAACTAATGTTGTAATCTCAAATCTGACATTCAAGAATTCACCCTTTTGGACCCTCCATCCTGTATATTGCAGCAAAGTCCTTGTTCAGAATGTGACAATCCTTGCACCATCTGATTCTCCAAACACCGATGGTATTGATCCAGATTCTTCATCTGAAGTTTGCATTGAAGATTGCTACATTAGCACAGGCGATGATCTAATTTCGATCAAGAGTGGGTGGGATGAATATGGTGTTGAGTATGCTCGTCCTAGTACGAACATTACCATTCGAAGGCTCACTGGGCAAACTACTAGTGCGGGAATTGCAATAGGAAGTGAGATGTCTGGAGGCGTGTCAGAAATTCTTGTAGAAAACATCCTATTTTTCAACTCAAACATGGGTATCAGAATTAAGACGTCTCCAGGAAGGGGTGGTTACGTGCGGAATGTTTACATATCGAATGTGAGCTTGATCGATGTAAACGTAGCTATAAGGTTCACTGGTAAGTATGGTGAGCACCCAGATTCTGATTATAATCCAAATGCTCTTCCGACAGTTGAAAGGATCACAATTAAACATGTTAGTGGAGAGAATATTAAAACAGCAGGCGTTTTAGAGGGCATAGAAGGAGATGATTTTCATAATATTTGCTTAGCCAATATCACGCTTAATGTGAGCTCAATGCACAAATGGAACTGTTCGCATGTGCAAGGATTTTCTGACTTTGTTTCTCCAAAGACATGCCATTCTCTGAGGGAAAGAATTTTTCCCCAACATATTTCGGATTGTTATCAGCCACCAAATTTCATTGCTCAGTCTCTTGTGTGA

Protein sequence

MLIAELSQMMHLIPSLLILLTIVALFFQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQSLV

Homology

[Close]

BLAST of Carg21200 vs. NCBI nr
Match: KAG7012696.1 (putative polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 973.4 bits (2515), Expect = 7.1e-280
Identity = 478/478 (100.00%), Postives = 478/478 (100.00%), Query Frame = 0


Query: 1   MLIAELSQMMHLIPSLLILLTIVALFFQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGE 60
           MLIAELSQMMHLIPSLLILLTIVALFFQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGE
Sbjct: 1   MLIAELSQMMHLIPSLLILLTIVALFFQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGE 60

Query: 61  IRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISH 120
           IRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISH
Sbjct: 61  IRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISH 120

Query: 121 LTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGT 180
           LTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGT
Sbjct: 121 LTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGT 180

Query: 181 IDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQN 240
           IDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQN
Sbjct: 181 IDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQN 240

Query: 241 VTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIR 300
           VTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIR
Sbjct: 241 VTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIR 300

Query: 301 RLTGQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL 360
           RLTGQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL
Sbjct: 301 RLTGQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL 360

Query: 361 IDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC 420
           IDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC
Sbjct: 361 IDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC 420

Query: 421 LANITLNVSSMHKWNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQSLV 479
           LANITLNVSSMHKWNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQSLV
Sbjct: 421 LANITLNVSSMHKWNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQSLV 478

[Close]

BLAST of Carg21200 vs. NCBI nr
Match: XP_022945524.1 (probable polygalacturonase isoform X2 [Cucurbita moschata])

HSP 1 Score: 930.6 bits (2404), Expect = 5.3e-267
Identity = 449/450 (99.78%), Postives = 450/450 (100.00%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 479
           KTCHSLRERIFPQHISDCYQPPNFIAQSLV
Sbjct: 427 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 456

[Close]

BLAST of Carg21200 vs. NCBI nr
Match: XP_023542119.1 (probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 927.2 bits (2395), Expect = 5.9e-266
Identity = 447/450 (99.33%), Postives = 449/450 (99.78%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVE+M
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVELM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 479
           KTCHSLRERIFPQHISDCYQPPNFIAQSLV
Sbjct: 427 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 456

[Close]

BLAST of Carg21200 vs. NCBI nr
Match: XP_022945523.1 (probable polygalacturonase isoform X1 [Cucurbita moschata])

HSP 1 Score: 925.6 bits (2391), Expect = 1.7e-265
Identity = 446/447 (99.78%), Postives = 447/447 (100.00%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQ 476
           KTCHSLRERIFPQHISDCYQPPNFIAQ
Sbjct: 427 KTCHSLRERIFPQHISDCYQPPNFIAQ 453

[Close]

BLAST of Carg21200 vs. NCBI nr
Match: XP_022967082.1 (probable polygalacturonase isoform X2 [Cucurbita maxima])

HSP 1 Score: 925.2 bits (2390), Expect = 2.2e-265
Identity = 447/450 (99.33%), Postives = 448/450 (99.56%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 479
           KTCHSLRERIFPQHISDCY PPNFIAQSLV
Sbjct: 427 KTCHSLRERIFPQHISDCYLPPNFIAQSLV 456

[Close]

BLAST of Carg21200 vs. ExPASy Swiss-Prot
Match: A7PZL3 (Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 SV=1)

HSP 1 Score: 459.9 bits (1182), Expect = 3.5e-128
Identity = 217/420 (51.67%), Postives = 292/420 (69.52%), Query Frame = 0


Query: 62  RPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHL 121
           R HS S+ DFG VGDG TLNTKAFQ+A+  L+ +  +GGA+L+VPAG+WLTGSF L SH 
Sbjct: 59  RAHSASLVDFGGVGDGQTLNTKAFQDAVSELSKYGSEGGAQLYVPAGKWLTGSFSLTSHF 118

Query: 122 TLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTI 181
           TL+L +DAV+L S     WP+IKPLPSYGRGR+    R+ SLI+G NLT+V+ITG+NGTI
Sbjct: 119 TLFLHRDAVLLASQDISQWPVIKPLPSYGRGRDAAAGRYTSLIFGTNLTDVIITGDNGTI 178

Query: 182 DGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNV 241
           DGQG +WW  FH   L YTRP+L+E+M S ++ ISNLT  NSP W +HPVY   +L+Q +
Sbjct: 179 DGQGGLWWQRFHGGKLKYTRPYLIELMYSADIQISNLTLLNSPSWNVHPVYSRNILIQGI 238

Query: 242 TILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRR 301
           TILAP  SPNTDGI+PDS +   IEDCYI +GDD +++KSGWDEYG+ Y  P+  + IRR
Sbjct: 239 TILAPVRSPNTDGINPDSCTNTRIEDCYIVSGDDCVAVKSGWDEYGIAYGMPTKQLVIRR 298

Query: 302 LTG-QTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL 361
           LT     SA IA+GSEMSGG+ ++  E+I+  NS  GIRIKT  GRGGYV+++Y+  +++
Sbjct: 299 LTCISPYSAVIALGSEMSGGIQDVRAEDIVAINSESGIRIKTGIGRGGYVKDIYVRGMTM 358

Query: 362 IDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC 421
             +  A   TG YG H D+ Y+P A P ++ I  + +  EN+  A  LEGI  D F  IC
Sbjct: 359 KTMKWAFWMTGNYGSHADNHYDPKAFPVIQGINYRDMVAENVSMAARLEGIPSDPFTGIC 418

Query: 422 LANITLNVSSMHK---WNCSHVQGFSDFVSPKTCHSLRERIFPQHISDCYQPPNFIAQSL 478
           ++N+T+++++  K   W C+ V+G S  V+P  C +L ++  P+  S C    NF A+SL
Sbjct: 419 ISNVTIHLAAKAKKVPWTCTDVEGISSGVTPTPCSTLPDQ-GPEKTSLC----NFPAESL 473

[Close]

BLAST of Carg21200 vs. ExPASy Swiss-Prot
Match: Q7M1E7 (Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 1.1e-28
Identity = 126/440 (28.64%), Postives = 191/440 (43.41%), Query Frame = 0


Query: 48  SSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPA 107
           S+ S  KL ++        ++  +GAVGDG   +T+AF       N+   K  A L VPA
Sbjct: 41  SNRSLKKLVHSRHDAATVFNVEQYGAVGDGKHDSTEAFATT---WNAACKKASAVLLVPA 100

Query: 108 GRW-----LTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRS 167
            +      L        HL+  +D        TI     + +P P+  +  ++       
Sbjct: 101 NKKFFVNNLVFRGPCQPHLSFKVD-------GTI-----VAQPDPARWKNSKI------- 160

Query: 168 LIYGCNLTNVVITGENGTIDGQGSIWW----DWFHSKTL--NYTRPHLVEIMNSTNVVIS 227
            +    LT+  + G  G IDGQG  WW       + +T+  +  RP  ++I  S +V + 
Sbjct: 161 WLQFAQLTDFNLMG-TGVIDGQGQQWWAGQCKVVNGRTVCNDRNRPTAIKIDYSKSVTVK 220

Query: 228 NLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDL 287
            LT  NSP + L    C  V +Q + I AP DSPNTDGID  +S    IE C I TGDD 
Sbjct: 221 ELTLMNSPEFHLVFGECEGVKIQGLKIKAPRDSPNTDGIDIFASKRFHIEKCVIGTGDDC 280

Query: 288 ISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGS----EMSGGVSEILVENILFF 347
           I+I +G           S+NITI+ L       GI+IGS         VS + V    F 
Sbjct: 281 IAIGTG-----------SSNITIKDLI-CGPGHGISIGSLGRDNSRAEVSHVHVNRAKFI 340

Query: 348 NSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPTVERI 407
           ++  G+RIKT  G  G    +   NV +I+    I     Y     +  N  +   ++ +
Sbjct: 341 DTQNGLRIKTWQGGSGLASYITYENVEMINSENPILINQFYCTSASACQNQRSAVQIQGV 400

Query: 408 TIKHVSGENIKTAGV-LEGIEGDDFHNICLANITLNVSSMHKWNC--SHVQGFSDFVSPK 467
           T K++ G +   A + L   +      I L+N++L ++S    +C   + +GF       
Sbjct: 401 TYKNIHGTSATAAAIQLMCSDSVPCTGIQLSNVSLKLTSGKPASCVDKNARGFYSGRLIP 445

Query: 468 TCHSLRERIFPQHISDCYQP 470
           TC +LR    P+      QP
Sbjct: 461 TCKNLRPGPSPKEFELQQQP 445

[Close]

BLAST of Carg21200 vs. ExPASy Swiss-Prot
Match: Q9FY19 (Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 1.8e-28
Identity = 118/418 (28.23%), Postives = 189/418 (45.22%), Query Frame = 0


Query: 67  SITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLD 126
           ++  +GAVGDG   +T AF+      N+  +K  A   VPA +       ++++L  +  
Sbjct: 61  NVEHYGAVGDGKHDSTDAFEKT---WNAACNKLSAVFLVPANKKF-----VVNNLVFYGP 120

Query: 127 KDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGS 186
                  S   D      P P+  +  ++        ++   LT+  + G  G IDGQG+
Sbjct: 121 CQPHF--SFKVDGTIAAYPDPAKWKNSKI-------WMHFARLTDFNLMG-TGVIDGQGN 180

Query: 187 IWWDWFHSKTLN-------YTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQ 246
            WW     KT+N         RP  ++I  S +V +  LT  NSP + L    C  V +Q
Sbjct: 181 RWWS-DQCKTINGRTVCNDKGRPTAIKIDFSKSVTVKELTLTNSPEFHLVFGECDGVKIQ 240

Query: 247 NVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITI 306
            + I AP DSPNTDGID  +S    IE C I TGDD +++ +G           S+NITI
Sbjct: 241 GIKIKAPRDSPNTDGIDIFASKRFEIEKCTIGTGDDCVAVGTG-----------SSNITI 300

Query: 307 RRLTGQTTSAGIAIGSEMSGG----VSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYI 366
           + LT      G++IGS   G     VS + ++   F ++  G+RIKT  G  G   ++  
Sbjct: 301 KDLT-CGPGHGMSIGSLGKGNSRSEVSFVHLDGAKFIDTQNGLRIKTWQGGSGLASHITY 360

Query: 367 SNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGV-LEGIEGD 426
            NV +I+    I     Y     +  N  +   ++ +T K++ G +  TA + L   +  
Sbjct: 361 ENVEMINAENPILINQFYCTSAAACKNQRSAVKIQDVTFKNIHGTSATTAAIQLMCSDSV 420

Query: 427 DFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP--KTCHSLRERIFPQHISDCYQPP 471
              NI L+N+ L ++S     C +      + +P   +C SL     P+ + + +Q P
Sbjct: 421 PCSNIKLSNVFLKLTSGKVATCVNKNANGYYTNPLNPSCKSLHPGRTPKEL-ELHQKP 446

[Close]

BLAST of Carg21200 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 125.9 bits (315), Expect = 1.2e-27
Identity = 108/356 (30.34%), Postives = 171/356 (48.03%), Query Frame = 0


Query: 64  HSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTL 123
           +++ +T FGAVGDGVT +++AF  A  +    +  G  +  VPAG            +T 
Sbjct: 22  NALDVTQFGAVGDGVTDDSQAFLKA--WEAVCSGTGDGQFVVPAG------------MTF 81

Query: 124 WLDKDAVILGSTISDDWPIIKPL------PSYGRGRELPGERHRSLIYGCNLTNVVITGE 183
            L +     GS  S   P+   +      PS G  +   G++ + +++  ++  +VI G+
Sbjct: 82  ML-QPLKFQGSCKST--PVFVQMLGKLVAPSKGNWK---GDKDQWILF-TDIEGLVIEGD 141

Query: 184 NGTIDGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVL 243
            G I+GQGS WW+   S      RP  ++  +  N+ +S LT  +SP   +H   C+ V 
Sbjct: 142 -GEINGQGSSWWEHKGS------RPTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVT 201

Query: 244 VQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNI 303
           + ++ I AP  SPNTDGID  +SS V I+DC I+TGDD I+I SG           ++NI
Sbjct: 202 ISSLRINAPESSPNTDGIDVGASSNVVIQDCIIATGDDCIAINSG-----------TSNI 261

Query: 304 TIRRLTGQTTSAGIAIGSEMSGG----VSEILVENILFFNSNMGIRIKTSPGRGGYVRNV 363
            I  +       GI+IGS    G    V  + V+N  F  +  G RIKT  G  GY R +
Sbjct: 262 HISGI-DCGPGHGISIGSLGKDGETATVENVCVQNCNFRGTMNGARIKTWQGGSGYARMI 321

Query: 364 YISNVSLIDVNVAIRFTGKY--GEHPDSDYNPNALPTVERITIKHVSGENIKTAGV 408
             + ++L +V   I     Y  G+  ++    ++   V ++   +  G +    GV
Sbjct: 322 TFNGITLDNVENPIIIDQFYNGGDSDNAKDRKSSAVEVSKVVFSNFIGTSKSEYGV 337

[Close]

BLAST of Carg21200 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 119.4 bits (298), Expect = 1.1e-25
Identity = 97/346 (28.03%), Postives = 149/346 (43.06%), Query Frame = 0


Query: 63  PHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGR-WLTGSFDLIS-- 122
           P +VS++DFGA GDG T +T+AF NA  +  + +  G   L VP G  +L  S  L    
Sbjct: 65  PTTVSVSDFGAKGDGKTDDTQAFVNA--WKKACSSNGAVNLLVPKGNTYLLKSIQLTGPC 124

Query: 123 HLTLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENG 182
           +  L +     +  S    D+  I     +     L                 V  G+ G
Sbjct: 125 NSILTVQIFGTLSASQKRSDYKDISKWIMFDGVNNLS----------------VDGGDTG 184

Query: 183 TIDGQGSIWW--DWFHSKTLNYTR-PHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKV 242
            +DG G  WW      +K    T+ P  +   NS ++++ NL  +N+    +    CS V
Sbjct: 185 VVDGNGETWWQNSCKRNKAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNV 244

Query: 243 LVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTN 302
            V NV + AP+DSPNTDGI   ++  + + +  I TGDD ISI+SG           S N
Sbjct: 245 QVSNVVVTAPADSPNTDGIHITNTQNIRVSESIIGTGDDCISIESG-----------SQN 304

Query: 303 ITIRRLT---GQTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNV 362
           + I  +T   G   S G          VS + V+      ++ G+RIKT  G  G   N+
Sbjct: 305 VQINDITCGPGHGISIGSLGDDNSKAFVSGVTVDGAKLSGTDNGVRIKTYQGGSGTASNI 364

Query: 363 YISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSG 400
              N+ + +V   I     Y +        +A+  V+ +  + +SG
Sbjct: 365 IFQNIQMDNVKNPIIIDQDYCDKSKCTTEKSAV-QVKNVVYRDISG 380

[Close]

BLAST of Carg21200 vs. ExPASy TrEMBL
Match: A0A6J1G174 (probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449733 PE=3 SV=1)

HSP 1 Score: 930.6 bits (2404), Expect = 2.6e-267
Identity = 449/450 (99.78%), Postives = 450/450 (100.00%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 479
           KTCHSLRERIFPQHISDCYQPPNFIAQSLV
Sbjct: 427 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 456

[Close]

BLAST of Carg21200 vs. ExPASy TrEMBL
Match: A0A6J1G153 (probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449733 PE=3 SV=1)

HSP 1 Score: 925.6 bits (2391), Expect = 8.3e-266
Identity = 446/447 (99.78%), Postives = 447/447 (100.00%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQ 476
           KTCHSLRERIFPQHISDCYQPPNFIAQ
Sbjct: 427 KTCHSLRERIFPQHISDCYQPPNFIAQ 453

[Close]

BLAST of Carg21200 vs. ExPASy TrEMBL
Match: A0A6J1HPS3 (probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111466589 PE=3 SV=1)

HSP 1 Score: 925.2 bits (2390), Expect = 1.1e-265
Identity = 447/450 (99.33%), Postives = 448/450 (99.56%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQSLV 479
           KTCHSLRERIFPQHISDCY PPNFIAQSLV
Sbjct: 427 KTCHSLRERIFPQHISDCYLPPNFIAQSLV 456

[Close]

BLAST of Carg21200 vs. ExPASy TrEMBL
Match: A0A6J1HR20 (probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111466589 PE=3 SV=1)

HSP 1 Score: 920.2 bits (2377), Expect = 3.5e-264
Identity = 444/447 (99.33%), Postives = 445/447 (99.55%), Query Frame = 0


Query: 29  LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 88
           LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA
Sbjct: 7   LVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNA 66

Query: 89  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 148
           IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS
Sbjct: 67  IFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPS 126

Query: 149 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 208
           YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM
Sbjct: 127 YGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIM 186

Query: 209 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 268
           NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC
Sbjct: 187 NSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDC 246

Query: 269 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 328
           YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN
Sbjct: 247 YISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVEN 306

Query: 329 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPT 388
           ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDS YNPNALPT
Sbjct: 307 ILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSGYNPNALPT 366

Query: 389 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDFVSP 448
           VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMH+WNCSHVQGFSDFVSP
Sbjct: 367 VERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHQWNCSHVQGFSDFVSP 426

Query: 449 KTCHSLRERIFPQHISDCYQPPNFIAQ 476
           KTCHSLRERIFPQHISDCY PPNFIAQ
Sbjct: 427 KTCHSLRERIFPQHISDCYLPPNFIAQ 453

[Close]

BLAST of Carg21200 vs. ExPASy TrEMBL
Match: A0A6J1DDC5 (probable polygalacturonase isoform X3 OS=Momordica charantia OX=3673 GN=LOC111019738 PE=3 SV=1)

HSP 1 Score: 813.9 bits (2101), Expect = 3.5e-232
Identity = 388/451 (86.03%), Postives = 420/451 (93.13%), Query Frame = 0


Query: 27  FQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQ 86
           F LVDVLLLLALFC++P +A  SSS+CKL N  EIRPHSVSITDFGAVGDG+TLNTKAFQ
Sbjct: 5   FTLVDVLLLLALFCLAPRIA-RSSSFCKLINVAEIRPHSVSITDFGAVGDGITLNTKAFQ 64

Query: 87  NAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPL 146
           NAIFYLNSFADKGGAKL VPAGRWLTGSFDLISHLTLWLDKDAVILGSTIS+DWP++KPL
Sbjct: 65  NAIFYLNSFADKGGAKLLVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISNDWPVVKPL 124

Query: 147 PSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVE 206
           PSYGRGRELPG+RHRSLIYGCNLT+V+ITGENGTIDGQGSIWWDWFH KTLNYTRPHLVE
Sbjct: 125 PSYGRGRELPGKRHRSLIYGCNLTDVIITGENGTIDGQGSIWWDWFHRKTLNYTRPHLVE 184

Query: 207 IMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIE 266
           +MNST VVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAP DSPNTDGIDPDSS+EVCIE
Sbjct: 185 LMNSTRVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPLDSPNTDGIDPDSSAEVCIE 244

Query: 267 DCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGVSEIL 326
           DCYISTGDDLISIKSGWD+YG+EYA PSTNITIRRLTGQT TSAG+AIGSEMSGG+SEI 
Sbjct: 245 DCYISTGDDLISIKSGWDKYGIEYAHPSTNITIRRLTGQTRTSAGVAIGSEMSGGISEIH 304

Query: 327 VENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNA 386
           VEN+LFFNS MGIRIKTSPGR GYVRNVYISNVSL +V++AIRFTG YGEHPDS Y+P A
Sbjct: 305 VENLLFFNSRMGIRIKTSPGRRGYVRNVYISNVSLFNVDIAIRFTGNYGEHPDSCYDPMA 364

Query: 387 LPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQGFSDF 446
           LPTVE ITIKHVSGENI+TAG+LEGIEGDDFHNICL+NITLNV+SMH WNCSHVQGFSD 
Sbjct: 365 LPTVEGITIKHVSGENIETAGLLEGIEGDDFHNICLSNITLNVTSMHHWNCSHVQGFSDL 424

Query: 447 VSPKTCHSLRERIFPQHISDCYQPPNFIAQS 477
           VSP+ C  L+E+I P+HI +CY PPNF+  S
Sbjct: 425 VSPEICQPLKEKISPRHILECYLPPNFLWNS 454

[Close]

BLAST of Carg21200 vs. TAIR 10
Match: AT3G62110.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 655.6 bits (1690), Expect = 3.1e-188
Identity = 314/447 (70.25%), Postives = 371/447 (83.00%), Query Frame = 0


Query: 27  FQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEI-RPHSVSITDFGAVGDGVTLNTKAF 86
           F L+ VLL+ A +    W +   S +C+ +N   + RPHSVSIT+FGAVGDGVTLNTKAF
Sbjct: 5   FLLLYVLLVQAFY--GAWCSVGESLHCEYSNLASLHRPHSVSITEFGAVGDGVTLNTKAF 64

Query: 87  QNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKP 146
           QNA+FYLNSF+DKGGAKLFVPAG+WLTGSFDLISHLTLWLDK A ILGST S++WP++ P
Sbjct: 65  QNALFYLNSFSDKGGAKLFVPAGQWLTGSFDLISHLTLWLDKGATILGSTSSENWPVVDP 124

Query: 147 LPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLV 206
           LPSYGRGRELPG RHRSLIYG NLT+VVITGENGTIDGQG++WWDWF +  LNYTRPHLV
Sbjct: 125 LPSYGRGRELPGRRHRSLIYGQNLTDVVITGENGTIDGQGTVWWDWFRNGELNYTRPHLV 184

Query: 207 EIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCI 266
           E+MNST ++ISNLTF NSPFW +HPVYC  V+V+N+TILAP +SPNTDG+DPDSS+ VCI
Sbjct: 185 ELMNSTGLIISNLTFLNSPFWNIHPVYCRDVVVKNLTILAPLESPNTDGVDPDSSTNVCI 244

Query: 267 EDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTT-SAGIAIGSEMSGGVSEI 326
           EDCYI TGDDL+SIKSGWDEYG+ YARPS+ I I RLTGQTT S+GIAIGSEMSGGVSEI
Sbjct: 245 EDCYIVTGDDLVSIKSGWDEYGISYARPSSKIKINRLTGQTTSSSGIAIGSEMSGGVSEI 304

Query: 327 LVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPN 386
            ++++  FNSN GIRIKTS GRGGYVRNV+I NV L +V  AIRFTGKYGEHPD  Y+P 
Sbjct: 305 YIKDLHLFNSNTGIRIKTSAGRGGYVRNVHILNVKLDNVKKAIRFTGKYGEHPDEKYDPK 364

Query: 387 ALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHK---WNCSHVQG 446
           ALP +E+IT ++V+G+ I  AG+LEGIEGD F NIC  N+TL V    K   W CS+V+G
Sbjct: 365 ALPAIEKITFENVNGDGIGVAGLLEGIEGDVFKNICFLNVTLRVKKNSKKSPWECSNVRG 424

Query: 447 FSDFVSPK-TCHSLRERIFPQHISDCY 468
           +S +VSP+ TC SL+E IFP+H SDC+
Sbjct: 425 YSQWVSPEITCDSLKESIFPEHGSDCF 449

[Close]

BLAST of Carg21200 vs. TAIR 10
Match: AT4G23820.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 476.1 bits (1224), Expect = 3.3e-134
Identity = 231/428 (53.97%), Postives = 301/428 (70.33%), Query Frame = 0


Query: 30  VDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNTKAFQNAI 89
           V + L   LF +S    G S + C        R   +SITDFG VGDG T+NTKAF+ AI
Sbjct: 6   VSIFLFSCLF-VSSSSLGDSEATCSGIVPLRYRYDKISITDFGGVGDGRTVNTKAFRAAI 65

Query: 90  FYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPIIKPLPSY 149
           + +     +GG  L++P G +LT SF+L SH+TL+L K AVI     + +WP+I PLPSY
Sbjct: 66  YRIQHLKRRGGTLLYIPPGVYLTESFNLTSHMTLYLAKGAVIRAVQDTWNWPLIDPLPSY 125

Query: 150 GRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRPHLVEIMN 209
           GRGRELPG R+ S I+G  L +VVITG+NGTIDGQG +WW+ + S+TL YTRP+L+E  +
Sbjct: 126 GRGRELPGGRYMSFIHGDGLRDVVITGQNGTIDGQGEVWWNMWRSRTLKYTRPNLIEFKD 185

Query: 210 STNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSEVCIEDCY 269
           S  ++ISN+ F+NSPFW +HPVYCS V++ +VTILAP DSPNTDGIDPDSS  VCIED Y
Sbjct: 186 SKEIIISNVIFQNSPFWNIHPVYCSNVVIHHVTILAPQDSPNTDGIDPDSSYNVCIEDSY 245

Query: 270 ISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQTTSAGIAIGSEMSGGVSEILVENI 329
           ISTGDDL++IKSGWD+YG+ Y RPS+NITIRR+TG +  AGIAIGSE SGG+  I+ E+I
Sbjct: 246 ISTGDDLVAIKSGWDQYGIAYGRPSSNITIRRITGSSPFAGIAIGSETSGGIKNIIAEHI 305

Query: 330 LFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDYNPNALPTV 389
              N  +G+ IKT+ GRGGY++N+ IS+V +      I+  G  G+HPD +YNPNALP V
Sbjct: 306 TLSNMGVGVNIKTNIGRGGYIKNIKISDVYVDTAKYGIKIAGDTGDHPDENYNPNALPVV 365

Query: 390 ERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITL--NVSSMHKWNCSHVQGFSDFVS 449
           + I IK+V G N++ AG ++G++G  F  ICL+ I L  +++S   W CS V G S  VS
Sbjct: 366 KGIHIKNVWGVNVRNAGSIQGLKGSPFTGICLSEINLHGSLNSYKTWKCSDVSGTSLKVS 425

Query: 450 PKTCHSLR 456
           P  C  LR
Sbjct: 426 PWPCSELR 432

[Close]

BLAST of Carg21200 vs. TAIR 10
Match: AT4G33440.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 473.8 bits (1218), Expect = 1.7e-133
Identity = 224/394 (56.85%), Postives = 295/394 (74.87%), Query Frame = 0


Query: 66  VSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWL 125
           +SITDFG VGDG T NT AF+ A+ +L  FA +GGA+L VP G WL+GSF+L S+ TL+L
Sbjct: 72  MSITDFGGVGDGKTSNTAAFRRAVRHLEGFAAEGGAQLNVPEGTWLSGSFNLTSNFTLFL 131

Query: 126 DKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQG 185
           ++ A+ILGS   D+WPII+PLPSYGRGRE PG RH SLI+G NLTNVVITGENGTIDGQG
Sbjct: 132 ERGALILGSKDLDEWPIIEPLPSYGRGRERPGGRHISLIHGDNLTNVVITGENGTIDGQG 191

Query: 186 SIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILA 245
            +WW+ + ++TL +TR HL+E+ NS N++ISNLT  NSPFWT+HPVYCS V+++N+TILA
Sbjct: 192 KMWWELWWNRTLVHTRGHLIELKNSHNILISNLTLLNSPFWTIHPVYCSNVVIRNMTILA 251

Query: 246 PSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQ 305
           P ++PNTDGIDPDSS+ VCIEDCYI +GDDL+++KSGWD+YG+  ARPS+NI IRR++G 
Sbjct: 252 PMNAPNTDGIDPDSSTNVCIEDCYIESGDDLVAVKSGWDQYGMAVARPSSNIVIRRISGT 311

Query: 306 T-TSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVN 365
           T T +G+ IGSEMSGG+  I VE+I  ++S  G+RIKT  GRGGY+ N+  +NV L  V 
Sbjct: 312 TRTCSGVGIGSEMSGGIFNITVEDIHVWDSAAGLRIKTDKGRGGYISNITFNNVLLEKVK 371

Query: 366 VAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANI 425
           V IRF+    +H D  ++P ALP V+ I I +V   N + A +L G+EG  F ++CL N+
Sbjct: 372 VPIRFSSGSNDHSDDKWDPKALPRVKGIYISNVVSLNSRKAPMLLGVEGTSFQDVCLRNV 431

Query: 426 T-LNVSSMHKWNCSHVQGFSDFVSPKTCHSLRER 458
           T L +    KW C  V G++  V P +C  L ++
Sbjct: 432 TLLGLPKTEKWKCKDVSGYASDVFPLSCPQLLQK 465

[Close]

BLAST of Carg21200 vs. TAIR 10
Match: AT3G16850.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 458.4 bits (1178), Expect = 7.2e-129
Identity = 227/433 (52.42%), Postives = 301/433 (69.52%), Query Frame = 0


Query: 23  VALFFQLVDVLLLLALFCISPWVAGSSSSYCKLTNTGEIRPHSVSITDFGAVGDGVTLNT 82
           VAL + L   +LL++           +++ CK     + RPHSVSI +FGAVGDG TLNT
Sbjct: 5   VALVWLLAFTILLIS--------GEGNNAICKENFKLDPRPHSVSILEFGAVGDGKTLNT 64

Query: 83  KAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHLTLWLDKDAVILGSTISDDWPI 142
            AFQNA+FYL SFADKGGA+L+VP G+WLTGSF+L SHLTL+L+K A IL S     W +
Sbjct: 65  IAFQNAVFYLKSFADKGGAQLYVPPGKWLTGSFNLTSHLTLFLEKGATILASPDPSHWDV 124

Query: 143 IKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTIDGQGSIWWDWFHSKTLNYTRP 202
           + PLPSYGRG ELPG+R+RSLI G NL +VVITGENGT DGQG+ WW+W  S +LNY+RP
Sbjct: 125 VSPLPSYGRGIELPGKRYRSLINGDNLIDVVITGENGTFDGQGAAWWEWLESGSLNYSRP 184

Query: 203 HLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNVTILAPSDSPNTDGIDPDSSSE 262
           H++E ++S +++ISNLTF N+P   +HPVYCS++ ++ V I    DSP+  G+ PDSS  
Sbjct: 185 HIIEFVSSKHILISNLTFLNAPAINIHPVYCSQIHIRKVLIETSVDSPHVLGVAPDSSDN 244

Query: 263 VCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRRLTGQT-TSAGIAIGSEMSGGV 322
           VCIED  I+ G D +S+KSGWD+YG+ Y RP+T + IR L  ++ T AGI+ GSEMSGGV
Sbjct: 245 VCIEDSTINVGHDAVSLKSGWDQYGIHYGRPTTAVHIRNLRLKSPTGAGISFGSEMSGGV 304

Query: 323 SEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSLIDVNVAIRFTGKYGEHPDSDY 382
           S++ VE +   +S +G+  +T+ GRGGY+RN+ IS+V L  V+ AI   G  G HPD  +
Sbjct: 305 SDVTVERLNIHSSLIGVAFRTTRGRGGYIRNITISDVDLTSVDTAIVANGHTGSHPDDKF 364

Query: 383 NPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNICLANITLNVSSMHKWNCSHVQG 442
           + +ALP V  I +++ +G +I  AG L GI    F +ICLA+I L   S   W CS+V G
Sbjct: 365 DRDALPVVTHIVMRNFTGVDIGVAGNLTGIGESPFTSICLADIHLQTRSEESWICSNVSG 424

Query: 443 FSDFVSPKTCHSL 455
           FSD VSP+ C  L
Sbjct: 425 FSDDVSPEPCQEL 429

[Close]

BLAST of Carg21200 vs. TAIR 10
Match: AT3G61490.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 452.6 bits (1163), Expect = 3.9e-127
Identity = 209/397 (52.64%), Postives = 285/397 (71.79%), Query Frame = 0


Query: 62  RPHSVSITDFGAVGDGVTLNTKAFQNAIFYLNSFADKGGAKLFVPAGRWLTGSFDLISHL 121
           R HS SIT++G VGDG TLNTKAFQ+A+ +L+ ++ +GGA+LFVPAG+WLTGSF+L SH 
Sbjct: 41  RSHSASITEYGGVGDGKTLNTKAFQSAVDHLSQYSSEGGAQLFVPAGKWLTGSFNLTSHF 100

Query: 122 TLWLDKDAVILGSTISDDWPIIKPLPSYGRGRELPGERHRSLIYGCNLTNVVITGENGTI 181
           TL+L KDA++L +   +++PI+K LPSYGRGR+  G R  SLI+G NL++V+ITG NGTI
Sbjct: 101 TLFLHKDAILLAAQDLNEYPILKALPSYGRGRDAAGGRFASLIFGTNLSDVIITGNNGTI 160

Query: 182 DGQGSIWWDWFHSKTLNYTRPHLVEIMNSTNVVISNLTFKNSPFWTLHPVYCSKVLVQNV 241
           DGQGS WW  FH   L YTRP+L+E+M S  + ISNLTF +SP W +HPVY S ++V+ V
Sbjct: 161 DGQGSFWWQKFHGGKLKYTRPYLIELMFSDTIQISNLTFLDSPSWNIHPVYSSNIIVKGV 220

Query: 242 TILAPSDSPNTDGIDPDSSSEVCIEDCYISTGDDLISIKSGWDEYGVEYARPSTNITIRR 301
           TI+AP  SPNTDGI+PDS +   IEDCYI +GDD I++KSGWDEYG+ +  P+ ++ IRR
Sbjct: 221 TIIAPVKSPNTDGINPDSCTNTRIEDCYIISGDDCIAVKSGWDEYGISFGMPTKHLVIRR 280

Query: 302 LTG-QTTSAGIAIGSEMSGGVSEILVENILFFNSNMGIRIKTSPGRGGYVRNVYISNVSL 361
           LT     SA IA+GSEMSGG+ ++  E+I  + +  G+RIKT+ GRG +V+N+Y+  ++L
Sbjct: 281 LTCISPYSAAIALGSEMSGGIEDVRAEDITAYQTESGVRIKTAVGRGAFVKNIYVKGMNL 340

Query: 362 IDVNVAIRFTGKYGEHPDSDYNPNALPTVERITIKHVSGENIKTAGVLEGIEGDDFHNIC 421
             +      TG Y  H DS Y+P+ALP +  I  + +  EN+  AG LEGI GD F  IC
Sbjct: 341 HTMKWVFWMTGNYKAHADSHYDPHALPEITGINYRDIVAENVSMAGRLEGISGDPFTGIC 400

Query: 422 LANITLNVSSMHK---WNCSHVQGFSDFVSPKTCHSL 455
           ++N T+++++ HK   W CS V+G +  V PK C  L
Sbjct: 401 ISNATISMAAKHKKAIWMCSDVEGVTSGVDPKPCDLL 437

The following BLAST results are available for this feature:

BLAST of Carg21200 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7012696.1	7.1e-280	100.00	putative polygalacturonase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022945524.1	5.3e-267	99.78	probable polygalacturonase isoform X2 [Cucurbita moschata]	[more]
XP_023542119.1	5.9e-266	99.33	probable polygalacturonase isoform X2 [Cucurbita pepo subsp. pepo]	[more]
XP_022945523.1	1.7e-265	99.78	probable polygalacturonase isoform X1 [Cucurbita moschata]	[more]
XP_022967082.1	2.2e-265	99.33	probable polygalacturonase isoform X2 [Cucurbita maxima]	[more]

BLAST of Carg21200 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A7PZL3	3.5e-128	51.67	Probable polygalacturonase OS=Vitis vinifera OX=29760 GN=GSVIVT00026920001 PE=1 ...	[more]
Q7M1E7	1.1e-28	28.64	Polygalacturonase OS=Chamaecyparis obtusa OX=13415 PE=1 SV=1	[more]
Q9FY19	1.8e-28	28.23	Polygalacturonase OS=Juniperus ashei OX=13101 GN=JNA2 PE=1 SV=1	[more]
Q9LW07	1.2e-27	30.34	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q8RY29	1.1e-25	28.03	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]

BLAST of Carg21200 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1G174	2.6e-267	99.78	probable polygalacturonase isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111449...	[more]
A0A6J1G153	8.3e-266	99.78	probable polygalacturonase isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111449...	[more]
A0A6J1HPS3	1.1e-265	99.33	probable polygalacturonase isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC11146658...	[more]
A0A6J1HR20	3.5e-264	99.33	probable polygalacturonase isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC11146658...	[more]
A0A6J1DDC5	3.5e-232	86.03	probable polygalacturonase isoform X3 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]

BLAST of Carg21200 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G62110.1	3.1e-188	70.25	Pectin lyase-like superfamily protein	[more]
AT4G23820.1	3.3e-134	53.97	Pectin lyase-like superfamily protein	[more]
AT4G33440.1	1.7e-133	56.85	Pectin lyase-like superfamily protein	[more]
AT3G16850.1	7.2e-129	52.42	Pectin lyase-like superfamily protein	[more]
AT3G61490.1	3.9e-127	52.64	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 321..342 e-value: 560.0 score: 9.0 coord: 233..259 e-value: 600.0 score: 8.8 coord: 294..315 e-value: 530.0 score: 9.2 coord: 260..292 e-value: 3300.0 score: 3.5 coord: 350..371 e-value: 790.0 score: 8.0
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 47..445 e-value: 1.2E-124 score: 418.4
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 161..440 e-value: 4.4E-46 score: 157.4
None	No IPR available	PANTHER	PTHR31339	PECTIN LYASE-RELATED	coord: 28..470
None	No IPR available	PANTHER	PTHR31339:SF20	OS07G0245200 PROTEIN	coord: 28..470
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 60..455

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21200-RA	Carg21200-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
molecular_function	GO:0004650	polygalacturonase activity