Carg21177 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21177
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionGlycosyltransferase
LocationCarg_Chr01: 11074483 .. 11074857 (+)
RNA-Seq ExpressionCarg21177
SyntenyCarg21177
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCCTCCCCGAAGCTTGCCGTCGTCAAAACCTCTCTGAAATTCCTTCTCTGCAGTTCTTCGTCTCTTTCCTCACTGGCACCACCCACCTCAAAAAACCCCTTTCAACAGCTCCTCCAATCCATGGCGGATTCCGGCGACCTCCCTTTGTGCGTCATCACTGATTACTTTCTTGGTTGGAACGCCAGCATTTGCCAAGACTTCCGCGTTCCAAGATTGGTCTTCCATGCCACCGTCGTTGTGCCATTTCTCATCACCAAAGCCATTATGGCCAATGCTGATAACACGGCTTCTGCTTCCTCTATCTCTACTCTTGTCCACCGGCTTCTGCCGAATCTGTCGTTTAATTTAACTGCCGCCGAATCTGTCGTTTAA

mRNA sequence

ATGGCCTCCCCGAAGCTTGCCGTCGTCAAAACCTCTCTGAAATTCCTTCTCTGCAGTTCTTCGTCTCTTTCCTCACTGGCACCACCCACCTCAAAAAACCCCTTTCAACAGCTCCTCCAATCCATGGCGGATTCCGGCGACCTCCCTTTGTGCGTCATCACTGATTACTTTCTTGGTTGGAACGCCAGCATTTGCCAAGACTTCCGCGTTCCAAGATTGGTCTTCCATGCCACCGTCGTTGTGCCATTTCTCATCACCAAAGCCATTATGGCCAATGCTGATAACACGGCTTCTGCTTCCTCTATCTCTACTCTTGTCCACCGGCTTCTGCCGAATCTGTCGTTTAATTTAACTGCCGCCGAATCTGTCGTTTAA

Coding sequence (CDS)

ATGGCCTCCCCGAAGCTTGCCGTCGTCAAAACCTCTCTGAAATTCCTTCTCTGCAGTTCTTCGTCTCTTTCCTCACTGGCACCACCCACCTCAAAAAACCCCTTTCAACAGCTCCTCCAATCCATGGCGGATTCCGGCGACCTCCCTTTGTGCGTCATCACTGATTACTTTCTTGGTTGGAACGCCAGCATTTGCCAAGACTTCCGCGTTCCAAGATTGGTCTTCCATGCCACCGTCGTTGTGCCATTTCTCATCACCAAAGCCATTATGGCCAATGCTGATAACACGGCTTCTGCTTCCTCTATCTCTACTCTTGTCCACCGGCTTCTGCCGAATCTGTCGTTTAATTTAACTGCCGCCGAATCTGTCGTTTAA

Protein sequence

MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAAESVV
Homology
BLAST of Carg21177 vs. NCBI nr
Match: KAG7037568.1 (hypothetical protein SDJN02_01196, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 236.5 bits (602), Expect = 1.2e-58
Identity = 124/124 (100.00%), Postives = 124/124 (100.00%), Query Frame = 0

Query: 1   MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGW 60
           MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGW
Sbjct: 1   MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGW 60

Query: 61  NASICQDFRVPRLVFHATVVVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAA 120
           NASICQDFRVPRLVFHATVVVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAA
Sbjct: 61  NASICQDFRVPRLVFHATVVVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAA 120

Query: 121 ESVV 125
           ESVV
Sbjct: 121 ESVV 124

BLAST of Carg21177 vs. NCBI nr
Match: KAG6608210.1 (Serine/arginine-rich splicing factor RSZ22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 228.4 bits (581), Expect = 3.4e-56
Identity = 119/121 (98.35%), Postives = 120/121 (99.17%), Query Frame = 0

Query: 1   MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGW 60
           MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGW
Sbjct: 1   MASPKLAVVKTSLKFLLCSSSSLSSLAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGW 60

Query: 61  NASICQDFRVPRLVFHATVVVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAA 120
           NASICQDFRVPRLVFHAT VVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAA
Sbjct: 61  NASICQDFRVPRLVFHATGVVPFLITKAIMANADNTASASSISTLVHRLLPNLSFNLTAA 120

Query: 121 E 122
           +
Sbjct: 121 D 121

BLAST of Carg21177 vs. NCBI nr
Match: XP_023523412.1 (UDP-glycosyltransferase 73B3-like, partial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 161.0 bits (406), Expect = 6.6e-36
Identity = 81/90 (90.00%), Postives = 85/90 (94.44%), Query Frame = 0

Query: 32  KNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMA 91
           KNPFQQLLQSMADSGDL LCVITDYFLGWNASICQ+F VPRLVFHAT VVP LITKAIMA
Sbjct: 101 KNPFQQLLQSMADSGDLSLCVITDYFLGWNASICQNFGVPRLVFHATGVVPLLITKAIMA 160

Query: 92  NADNTASASSISTLVHRLLPNLSFNLTAAE 122
           NAD TASASSISTL+HRLLP+LSFNLTAA+
Sbjct: 161 NADYTASASSISTLIHRLLPSLSFNLTAAD 190

BLAST of Carg21177 vs. NCBI nr
Match: XP_038900000.1 (UDP-glycosyltransferase 73B3-like [Benincasa hispida])

HSP 1 Score: 125.2 bits (313), Expect = 4.0e-25
Identity = 60/90 (66.67%), Postives = 72/90 (80.00%), Query Frame = 0

Query: 32  KNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMA 91
           + PFQQ LQSMA+SGD PLCV+TD+FL W   IC+ F +PRL FHAT VV  LITK IMA
Sbjct: 122 QTPFQQFLQSMAESGDRPLCVVTDFFLSWTLEICRRFDIPRLAFHATGVVSLLITKTIMA 181

Query: 92  NADNTASASSISTLVHRLLPNLSFNLTAAE 122
           N D TAS+SSIS L+H+LLPNL F++TAA+
Sbjct: 182 NTDYTASSSSISDLLHQLLPNLPFHVTAAD 211

BLAST of Carg21177 vs. NCBI nr
Match: QWT69379.1 (UGT73B4 [Gynostemma pentaphyllum])

HSP 1 Score: 85.1 bits (209), Expect = 4.6e-13
Identity = 46/93 (49.46%), Postives = 59/93 (63.44%), Query Frame = 0

Query: 26  LAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLI 85
           +A    + PF+Q+LQSM +S DLP+CVI+D+FL W   ICQ F V RLVFH   V+P LI
Sbjct: 92  IATKRFQKPFEQVLQSMKNSDDLPICVISDFFLTWTLGICQRFGVLRLVFHGMGVLPLLI 151

Query: 86  TKAIMANADNTASASSISTLVHRLLPNLSFNLT 119
           +K +  N   + S SS  +    LL NL FNLT
Sbjct: 152 SKTLWTNKLQSKSKSSSVSDHLNLLGNLPFNLT 184

BLAST of Carg21177 vs. ExPASy TrEMBL
Match: A0A2G5ET00 (Glycosyltransferase OS=Aquilegia coerulea OX=218851 GN=AQUCO_00400009v1 PE=3 SV=1)

HSP 1 Score: 82.4 bits (202), Expect = 1.4e-12
Identity = 42/87 (48.28%), Postives = 56/87 (64.37%), Query Frame = 0

Query: 32  KNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMA 91
           + PF+Q+LQ M++SG LP+CVI+DYFL W  S C  F VPR VFH T V+   ITK+I A
Sbjct: 101 QQPFEQILQDMSESGSLPICVISDYFLLWTVSSCHKFNVPRFVFHGTGVLGMAITKSIFA 160

Query: 92  NADNTASASSISTLVHRLLPNLSFNLT 119
           +  N  +  S + L+H    +L F LT
Sbjct: 161 HGSN-ITIKSETDLIHIPDVDLPFTLT 186

BLAST of Carg21177 vs. ExPASy TrEMBL
Match: A0A1U8AKW8 (Glycosyltransferase OS=Nelumbo nucifera OX=4432 GN=LOC104605345 PE=3 SV=1)

HSP 1 Score: 79.0 bits (193), Expect = 1.6e-11
Identity = 39/81 (48.15%), Postives = 53/81 (65.43%), Query Frame = 0

Query: 32  KNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMA 91
           + PF+Q+L+ M++ G LP+CVI+D+FLGW A+ C+ F VPRLVFH   V+P LI K +  
Sbjct: 99  QQPFEQILREMSEVGSLPICVISDFFLGWTATACRPFGVPRLVFHGMGVLPMLICKTLYT 158

Query: 92  NAD---NTASASSISTLVHRL 110
           N     + AS SS   LV  L
Sbjct: 159 NKTYLRSVASDSSSEALVLNL 179

BLAST of Carg21177 vs. ExPASy TrEMBL
Match: A0A2P6PZR8 (Glycosyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0305151 PE=3 SV=1)

HSP 1 Score: 78.2 bits (191), Expect = 2.7e-11
Identity = 36/75 (48.00%), Postives = 49/75 (65.33%), Query Frame = 0

Query: 26  LAPPTSKNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLI 85
           LA    + PF+Q+LQSM DS  LP+CVI+D+FLGW    CQDF VPRLVFH   V+   I
Sbjct: 97  LATKQLQKPFEQVLQSMRDSKSLPMCVISDFFLGWTLKYCQDFGVPRLVFHGMGVLSMAI 156

Query: 86  TKAIMANADNTASAS 101
            K+++ +  +  + S
Sbjct: 157 CKSVLVHRPHLKAKS 171

BLAST of Carg21177 vs. ExPASy TrEMBL
Match: A0A200QZ31 (Glycosyltransferase OS=Macleaya cordata OX=56857 GN=BVC80_1829g36 PE=3 SV=1)

HSP 1 Score: 77.4 bits (189), Expect = 4.6e-11
Identity = 36/90 (40.00%), Postives = 55/90 (61.11%), Query Frame = 0

Query: 32  KNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMA 91
           + PF Q+LQ M+++G LP+CVI+D+FL W  + C+ F VPR+VFH   V+  +I+K +  
Sbjct: 105 QEPFDQILQDMSNTGSLPICVISDFFLEWTLATCRKFNVPRIVFHGMGVLSMVISKYVWV 164

Query: 92  NADNTASASSISTLVHRLLPNLSFNLTAAE 122
           NA+   + S     +     NL F LT A+
Sbjct: 165 NAEQLITESDSEAPLQLSDLNLPFTLTKAD 194

BLAST of Carg21177 vs. ExPASy TrEMBL
Match: A0A7J6V4B4 (Glycosyltransferase OS=Thalictrum thalictroides OX=46969 GN=FRX31_031423 PE=3 SV=1)

HSP 1 Score: 77.0 bits (188), Expect = 6.1e-11
Identity = 39/87 (44.83%), Postives = 54/87 (62.07%), Query Frame = 0

Query: 32  KNPFQQLLQSMADSGDLPLCVITDYFLGWNASICQDFRVPRLVFHATVVVPFLITKAIMA 91
           + PF+++LQ M++SG LP+CVI+DYF  W  S C+ F VPR VFH T V+   ITK++ A
Sbjct: 105 QQPFEKILQEMSESGSLPICVISDYFFMWTISSCRKFNVPRFVFHGTGVLGMAITKSVYA 164

Query: 92  NADNTASASSISTLVHRLLPNLSFNLT 119
           +  N  S  S +  +H     L F LT
Sbjct: 165 HGSN-ISIGSQTDPIHIPDVELPFTLT 190

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7037568.11.2e-58100.00hypothetical protein SDJN02_01196, partial [Cucurbita argyrosperma subsp. argyro... [more]
KAG6608210.13.4e-5698.35Serine/arginine-rich splicing factor RSZ22, partial [Cucurbita argyrosperma subs... [more]
XP_023523412.16.6e-3690.00UDP-glycosyltransferase 73B3-like, partial [Cucurbita pepo subsp. pepo][more]
XP_038900000.14.0e-2566.67UDP-glycosyltransferase 73B3-like [Benincasa hispida][more]
QWT69379.14.6e-1349.46UGT73B4 [Gynostemma pentaphyllum][more]
Match NameE-valueIdentityDescription
Match NameE-valueIdentityDescription
A0A2G5ET001.4e-1248.28Glycosyltransferase OS=Aquilegia coerulea OX=218851 GN=AQUCO_00400009v1 PE=3 SV=... [more]
A0A1U8AKW81.6e-1148.15Glycosyltransferase OS=Nelumbo nucifera OX=4432 GN=LOC104605345 PE=3 SV=1[more]
A0A2P6PZR82.7e-1148.00Glycosyltransferase OS=Rosa chinensis OX=74649 GN=RchiOBHm_Chr6g0305151 PE=3 SV=... [more]
A0A200QZ314.6e-1140.00Glycosyltransferase OS=Macleaya cordata OX=56857 GN=BVC80_1829g36 PE=3 SV=1[more]
A0A7J6V4B46.1e-1144.83Glycosyltransferase OS=Thalictrum thalictroides OX=46969 GN=FRX31_031423 PE=3 SV... [more]
Match NameE-valueIdentityDescription
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.40.50.2000Glycogen Phosphorylase B;coord: 11..121
e-value: 1.1E-10
score: 43.5
NoneNo IPR availablePANTHERPTHR48047GLYCOSYLTRANSFERASEcoord: 31..121
NoneNo IPR availablePANTHERPTHR48047:SF26GLYCOSYLTRANSFERASEcoord: 31..121
NoneNo IPR availableSUPERFAMILY53756UDP-Glycosyltransferase/glycogen phosphorylasecoord: 29..111

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21177-RACarg21177-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
molecular_function GO:0016740 transferase activity