Carg21159 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21159
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ribosomal RNA large subunit methyltransferase H
Location	Carg_Chr01: 10978591 .. 10982765 (+)
RNA-Seq Expression	Carg21159
Synteny	Carg21159

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TAATTTTAATCAAATTTGTTCTCTCTTTTTCTCACGGTGGCAACTTGCTAACTGACACGTCATCATCTCCGTCTTAGCCCGAGGGGTCTGGTTTCTGCTATCGGCTTCTTATCAATGGTTACTGCTCTCTGTGCTACCATTTCCGCTTCTCCTTCAGGTATCATCGTTTTCTCTACAATAGAAGGAAAAAGAAACCCTAGAATTTGACCTAGGTTTTGTTCGTCTATGTTCTTACCGGCGTTGTGTAATGTTTCTCCGATTTCGAGCTTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGGTACATTTCTTGTTTCTTTAAATTTAGTTTCGTTGTTTGGTTCTTGTGATTTTTGTCTTCGTTGTGATTCTTTTTCCTTTTTACGATCTCGATCGTTCGATTGACGTTTCGTTGTGTTTTGCAATTCCATTTATTAATATCTGATATATTTGGCTGAGATTTCAGTGTGGTTGAGGCTCAGGATGACTGAGTTTTAACTGAGATTAGTCTTTTATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGATGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGTATGTGATTGTACGGTTGGTTTTCATTTATCAGTTTTCCTATTTACGCTCGAACTTCCAATCTCTGATGAATGATGCTAATCTTAATGAATGCTTTTCATTCCATGTTTCGTTTCTGCATTATAGGGATCCAAGAGCTCAGGTTGATGATGAGGACATAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTTGTCTCTTAACATTCATTTTCGTTGAATCATTACGAGTTTTGTTATTCTGGCTGTGCTCTTTTATTTGGTAACTAGTTTAGAACTCTCTTTGTGAGACCTCACGTCGATTAAAGAGGGGAACAAATGCCAGAGAGGACACTGGACTCCAAAGGGGGTGGATTGTGAAATCCCACGCTGGTTGGAGAGGGGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCTCTAGTTTAGAACTAGTTTAGAGCTGTCCCTGTGAGATCCCACATCGATTGAAAACGGGGAACGAATGCCAACGAGGACGCTGGACCCCAAAAGGGGTGGATTGTGAGATCCCACATCGGTTGGAGAGGGAAACAAAGCATTCTTTGTAAGGGCGTGGAAACTTCTCTCTAGCATATGCATTTTAAAACCTTGAGGGAAGCCTGAAAGGGAAAGCCCAAAGAAGACAATATCTACTAGTGGTGGACTTGGACGGTTACAAATCTCTCTCTCCGTCCCTCAATTACATCAGTCTTTTGCCAGCTAAAATGTGAAAGAAAATTGATCAGAGTGCTTCTTTTTCAGAAAAGAAATATAGTTTGCTATGTGCTTTGATTTTATTTTTAGCCCTTCTTCTAAAACTGATCTTTGAAATAGTTTATAATGGTCGTGCTTACTGCTTACAGTGTGTACCATATCCATTCTGCATATGCACCTTCAGGTTGTGATGTTGGATGAGAATGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGTGAGTACCATTTCCAATTCAAATCAAATGGGTTGCATTGTTATCATCAATTCATCTCTACTGGACTGATTCCTAATAGTTCAAAGTTCAAAGTTAGTGGCTAAGGCCCCATTTGAGAACGAATTGGTTTGGTTTTTTGTTTTCGAAAATTAAGCTTATAAATACTACTTCCACCTATGGATTTCCTTGTTTTGTTATCCACTTGTTACGTCTATTTTAAAAAATCGAGCCAAAGTTTTGAAAAACTAAGAAAACTAGTTTTCAAAAACTTGTTTTTGTCTTTGGATCTTGGCTAAGAATTTGTTTTCTCAAGAGAAATGATACGAAAATAAACACAATTTTCAAAAACAAAATAGTTATCTAACAGGAGTTTTTGCTTGAGGCACCTCTAAGTTAAACCAGCCCACATGTTCAACCTCCATGTCAGTCTAGATCTTAATATGTAACAGTCTCCTGCTTTCTAATTTGATGTTAAGAAAGTTTTGTATTAATCACCATGGTTTAATTGTTTCTGTAATCAAATTTGTTAGAACTATGGGTGTATCCCAGAATGAAAATGGCATTTCTTATGTTTCTTCTTCTTTGCAGGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGTACTTTTTTTGTTTCTTCTCTTCTAATCATTAGTGATTTCGAGTTTCATTTGCAACATCAAATGCTCCTCAAGCAGAGCTTTCCCAAAATGATGTGTTGGGTGGAGTATCCACTTACATTAATGTGATCTAGTACAATAAAGTAACAAGTACCACATAGTGAATATATAGAAATCCAAATCCAAATCCACCGAATTACTCCGCTTATGATCTTCTACATTCTAGGTCTTCCCGTTGCTTCATCTTATTTATGTTCTTCCTATAGCATTTAGACCGAATGACTATGAAATTACGTCGATACTTCTTCATATTACGAATATCGTAGGAAACATCTCTATTTTTTTCTCTTGAGAATCCTTAGAATTACGACTTCGATAATAATGAACTCAAGAGGTTAGACGTATAAAATACTGTGGGACTTTCAGTTTTGCTCAAATCAGCCAAGAATCATGATGCTCCTCTTTCTCTCTCTATCTCCATCTAGAGATTTATTCAAACCTCAATTCAACCCTGAAAAACAGACAAAACTAACAATGCACCATCTTTGCATCTGCGAAGCTTTTTGTATTATATAATGCTTATTCTGAATGTATGTATTCTTTTAGGTCATGGACTATTCTGAAGGGTCAGAAGTACCATCATTAGTAGCAGTGTTCGTACGGTATTTTCATATCTTTTTTTATTTATTGGAAGAGACCTTTTAATGGTAGTTTATTATTGTGTCGGGATGAATATTTAGAAGTTTAAATATCATTTTGGTCCATGTACTTTTAGTCTCATTTCATTATTATAGTCTCTTACAAACAAACTCGTACTTCTTGATAGTTTGTCGTCGGGACGGGGCAGGGGCTTCCTCTGCCATCACCATCAGACCTATCTAGTGAGTTCTTGTGGAGTGGGTCGGATACCTAATTTGGCTGTAGCACATCAGCTAAATATAGAGGGTGAGGGGAGTTTCATCCCGAGGCTATCCCGAGGCTGCCCCTTCAAGCAGTATTCACCTTAAAAAAAAAAAAATTAAGAAAATTTGTTATAGGTGACAAAATTAATTGTTAGTTTTGTGTTTTTAAATATTATGTTTATTTCTTCATATTTTTTCAATATATTTTTCACCTTTTCATAAAAACATATTTCATTTCATGGCAAAATTTCAAAATCACGAACAATTTATTATTACTACTACTTTATTTTCAAAAACAAATTGTTTAAGATTTTGAAAATATTTATAAAATGTAGTGGATAAATGTTTAAACTTAATTTTTAAACCTAAAAAACAAAAATAAAATAGTTAGCAAACGTCAGGCGTAAGTTGTATGGGTTTATAAATATAATTTTATATGTTATTTGTGAATTTTAAAAAAAAATTCACAATGTATGAAAAATAATTTAACATATTAAATTAGAGGTCGGCTTGAATTATCGGACCGCTAGAACGGTGTTCGTATCAACGGTCATGTTGAAAATTACGCGCGTTTTATTAAACGGGAAAATTGTAAGGTGGCGGCAAACAAGTCATCACGTGGACCTAATTCAAACGTATCAACGCCACGTCACAGCCAACTATAATACACGTGTCATCATCTCAGCTAATTAAATCTTCACAGGGCAACTTAAGACCATATCAGCATTTCAACAATGGAATCCCATTCTCGTCTCTTCAATTGTTGAGAACACTCCCTACTTCTTCCTCTCTGAAACCTGCGGTCCAAATTGCCTCAAAATCTGCTCGCCATCAATTTTTCCGACGAATCTCGACCATGAATTCGAGATCGAAGCTAGATTCTAG

mRNA sequence

TAATTTTAATCAAATTTGTTCTCTCTTTTTCTCACGGTGGCAACTTGCTAACTGACACGTCATCATCTCCGTCTTAGCCCGAGGGGTCTGGTTTCTGCTATCGGCTTCTTATCAATGGTTACTGCTCTCTGTGCTACCATTTCCGCTTCTCCTTCAGGTATCATCGTTTTCTCTACAATAGAAGGAAAAAGAAACCCTAGAATTTGACCTAGGTTTTGTTCGTCTATGTTCTTACCGGCGTTGTGTAATGTTTCTCCGATTTCGAGCTTTCAGTTGGACGTTGCAAGTACACCGGTCAATCTGTGGTACATTTCTTGTTTCTTTAAATTTAGTTTCGTTGTTTGGTTCTTGTGATTTTTGTCTTCGTTGTGATTCTTTTTCCTTTTTACGATCTCGATCGTTCGATTGACGTTTCGTTGTGTTTTGCAATTCCATTTATTAATATCTGATATATTTGGCTGAGATTTCAGTGTGGTTGAGGCTCAGGATGACTGAGTTTTAACTGAGATTAGTCTTTTATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGATGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGGATCCAAGAGCTCAGGTTGATGATGAGGACATAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTGTGATGTTGGATGAGAATGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGGGCTTCCTCTGCCATCACCATCAGACCTATCTAGTGAGTTCTTGTGGAGTGGGTCGGATACCTAATTTGGCTGTAGCACATCAGCTAAATATAGAGGGGCAACTTAAGACCATATCAGCATTTCAACAATGGAATCCCATTCTCGTCTCTTCAATTGTTGAGAACACTCCCTACTTCTTCCTCTCTGAAACCTGCGGTCCAAATTGCCTCAAAATCTGCTCGCCATCAATTTTTCCGACGAATCTCGACCATGAATTCGAGATCGAAGCTAGATTCTAG

Coding sequence (CDS)

ATGGACAATGAACAGAGAGCGGTGCCGATACGCGTTTTAACTGTGGGGAAGAAGAGGTCTCGTGGCGTTCAGCTTCTCATTGATGAGTATATTGACAAGCTCAATAACTACTGTAATGTGGAAGATGTTCAACTGCGATCAAATCCTAGAAATGCGCGGGATCCAAGAGCTCAGGTTGATGATGAGGACATAGCTGTTGTGAATCTTCTCAAGTCCGACGATTGGGTTGTGATGTTGGATGAGAATGGGAAAGAGATATCCTCCGAGCAGATGGCTGAGTTGGTGGCAGATGCAGGCAATACAGGTGCTTCAAGATTAGCATTTTGCATTGGTGGACCTTATGGCCATGGACAACAACTGCGACAACGAGCTAACGTATCGATCAAGCTGTCCTCCATGGTCTTAAATCATCAAATTGCATTACTTGTGCTTATGGAACAACTTTACAGGGGCTTCCTCTGCCATCACCATCAGACCTATCTAGTGAGTTCTTGTGGAGTGGGTCGGATACCTAATTTGGCTGTAGCACATCAGCTAAATATAGAGGGGCAACTTAAGACCATATCAGCATTTCAACAATGGAATCCCATTCTCGTCTCTTCAATTGTTGAGAACACTCCCTACTTCTTCCTCTCTGAAACCTGCGGTCCAAATTGCCTCAAAATCTGCTCGCCATCAATTTTTCCGACGAATCTCGACCATGAATTCGAGATCGAAGCTAGATTCTAG

Protein sequence

MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTYLVSSCGVGRIPNLAVAHQLNIEGQLKTISAFQQWNPILVSSIVENTPYFFLSETCGPNCLKICSPSIFPTNLDHEFEIEARF

Homology

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BLAST of Carg21159 vs. NCBI nr
Match: KAG7037551.1 (putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 489.2 bits (1258), Expect = 2.1e-134
Identity = 242/242 (100.00%), Postives = 242/242 (100.00%), Query Frame = 0


Query: 1   MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVD 60
           MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVD
Sbjct: 1   MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVD 60

Query: 61  DEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQL 120
           DEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQL
Sbjct: 61  DEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQL 120

Query: 121 RQRANVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTYLVSSCGVGRIPNLAVAHQLN 180
           RQRANVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTYLVSSCGVGRIPNLAVAHQLN
Sbjct: 121 RQRANVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTYLVSSCGVGRIPNLAVAHQLN 180

Query: 181 IEGQLKTISAFQQWNPILVSSIVENTPYFFLSETCGPNCLKICSPSIFPTNLDHEFEIEA 240
           IEGQLKTISAFQQWNPILVSSIVENTPYFFLSETCGPNCLKICSPSIFPTNLDHEFEIEA
Sbjct: 181 IEGQLKTISAFQQWNPILVSSIVENTPYFFLSETCGPNCLKICSPSIFPTNLDHEFEIEA 240

Query: 241 RF 243
           RF
Sbjct: 241 RF 242

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BLAST of Carg21159 vs. NCBI nr
Match: XP_022940478.1 (putative RNA methyltransferase At5g10620 [Cucurbita moschata] >KAG6608193.1 putative RNA methyltransferase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.1 bits (723), Expect = 2.2e-72
Identity = 147/155 (94.84%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

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BLAST of Carg21159 vs. NCBI nr
Match: XP_023524400.1 (putative RNA methyltransferase At5g10620 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 278.1 bits (710), Expect = 7.2e-71
Identity = 144/155 (92.90%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDVA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDE GKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDEKGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIALLVLMEQLY  +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIALLVLMEQLYSSWTILKGQKY 180

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BLAST of Carg21159 vs. NCBI nr
Match: XP_022982492.1 (putative RNA methyltransferase At5g10620 [Cucurbita maxima] >XP_022982493.1 putative RNA methyltransferase At5g10620 [Cucurbita maxima])

HSP 1 Score: 276.2 bits (705), Expect = 2.7e-70
Identity = 143/155 (92.26%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRN RD RAQVDDEDIA
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNTRDARAQVDDEDIA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIA+LVLMEQLYR +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIAVLVLMEQLYRSWTILKGQKY 180

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BLAST of Carg21159 vs. NCBI nr
Match: XP_038896820.1 (putative RNA methyltransferase At5g10620 isoform X1 [Benincasa hispida])

HSP 1 Score: 275.0 bits (702), Expect = 6.1e-70
Identity = 138/156 (88.46%), Postives = 149/156 (95.51%), Query Frame = 0


Query: 1   MDNEQRAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVD 60
           MDN QRAVPIRVLTVGKKRSRGVQLL+DEYI+KL NYC++EDV LRSNPRNARDPRAQVD
Sbjct: 24  MDNGQRAVPIRVLTVGKKRSRGVQLLVDEYIEKLKNYCHIEDVLLRSNPRNARDPRAQVD 83

Query: 61  DEDIAVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQL 120
           DED+AVVNLLKSDDWVVMLDENGKE+SSEQMA+LVADAG TGASRL+FCIGGPYGHGQQL
Sbjct: 84  DEDMAVVNLLKSDDWVVMLDENGKEMSSEQMADLVADAGTTGASRLSFCIGGPYGHGQQL 143

Query: 121 RQRANVSIKLSSMVLNHQIALLVLMEQLYRGFLCHH 157
           RQRAN+SIKLSSMVLNHQIALLVLMEQLYR  + +H
Sbjct: 144 RQRANLSIKLSSMVLNHQIALLVLMEQLYRVLVLYH 179

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BLAST of Carg21159 vs. ExPASy Swiss-Prot
Match: Q9LXB4 (Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g10620 PE=3 SV=1)

HSP 1 Score: 214.2 bits (544), Expect = 1.7e-54
Identity = 106/155 (68.39%), Postives = 132/155 (85.16%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RA+PIRV+TVGKKRS GV+LL+DEY  KL  YC+ ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           ++ L+ SDDWVV+LDE G++I SEQMAEL+ DAGN+GASR++FCIGG YGHG Q+R+RAN
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRAN 156

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           V+I+LSSMVLNHQIAL+VLMEQLYR +     Q Y
Sbjct: 157 VTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNY 191

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BLAST of Carg21159 vs. ExPASy Swiss-Prot
Match: A0M0D0 (Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0803) OX=411154 GN=rlmH PE=3 SV=1)

HSP 1 Score: 95.5 bits (236), Expect = 8.7e-19
Identity = 56/156 (35.90%), Postives = 87/156 (55.77%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVE-----DVQLRSNPRNARDPRAQVDDEDI 69
           I+++ +GK   + ++ LI  Y D+L +Y   E     D++   N     D   Q   E  
Sbjct: 3   IKLVCIGKTDKKELEALIKIYSDRLQHYIKFEFEIIPDLKKTKN----LDENQQKSKEGE 62

Query: 70  AVVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRA 129
            +++ +++ D+VV+LDENGK+ SSE  +E +    NTG  RL F IGGPYG  +++ +RA
Sbjct: 63  LILSGVQNSDFVVLLDENGKQFSSESFSEYIQKRMNTGLKRLIFVIGGPYGFSEEVYKRA 122

Query: 130 NVSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +  I LS M  +HQ+  L   EQLYR F    ++ Y
Sbjct: 123 DSKISLSKMTFSHQMVRLFFTEQLYRAFTILKNEPY 154

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BLAST of Carg21159 vs. ExPASy Swiss-Prot
Match: A0PXE8 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT) OX=386415 GN=rlmH PE=3 SV=1)

HSP 1 Score: 93.2 bits (230), Expect = 4.3e-18
Identity = 54/155 (34.84%), Postives = 87/155 (56.13%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQL--RSNPRNA--RDPRAQVDDEDIA 69
           I ++ VGK + + ++L IDEY  +L+ YC +  V+L     P NA  +D       E   
Sbjct: 3   ITIICVGKLKEKYLKLAIDEYSKRLSRYCKLNIVELNDEKTPDNASEKDELIIKQKEGSK 62

Query: 70  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 129
           ++  +K + +V+ LD NGK  SSE++AEL+ D G  G S +A  IGG  G  +++  RAN
Sbjct: 63  ILAHVKENMYVIALDLNGKMASSEELAELIGDLGLKGKSNIALVIGGSLGLSKEVLNRAN 122

Query: 130 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
             +  S M   HQ+  ++L+EQ+YR +  +  + Y
Sbjct: 123 YKLSFSKMTFPHQLMRVILLEQIYRAYKINAGEPY 157

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BLAST of Carg21159 vs. ExPASy Swiss-Prot
Match: B2V1Q9 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain Alaska E43 / Type E3) OX=508767 GN=rlmH PE=3 SV=1)

HSP 1 Score: 91.7 bits (226), Expect = 1.3e-17
Identity = 53/155 (34.19%), Postives = 90/155 (58.06%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYC--NVEDVQLRSNPRNA--RDPRAQVDDEDIA 69
           I +++VGK + + ++  IDEY  +L+ YC  N+ ++Q    P NA  +D     D E   
Sbjct: 3   ITIISVGKLKEKYLKHAIDEYSKRLSRYCKLNIVELQDEQTPDNASEKDELIIKDKEGNK 62

Query: 70  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 129
           ++N +K + +V+ LD  GK ISSE++++ + + G  G S L F IGG  G  + + +R+N
Sbjct: 63  ILNSIKDNMYVITLDLKGKMISSEELSKFIDNCGVRGNSNLCFIIGGSLGLSEAVLKRSN 122

Query: 130 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
            S+  S M   HQ+  ++L+EQ+YR F   + + Y
Sbjct: 123 YSLCFSKMTFPHQLFRVMLLEQIYRAFRISNGEPY 157

[Close]

BLAST of Carg21159 vs. ExPASy Swiss-Prot
Match: A7FZD1 (Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain ATCC 19397 / Type A) OX=441770 GN=rlmH PE=3 SV=1)

HSP 1 Score: 90.1 bits (222), Expect = 3.7e-17
Identity = 51/147 (34.69%), Postives = 85/147 (57.82%), Query Frame = 0


Query: 10  IRVLTVGKKRSRGVQLLIDEYIDKLNNYC--NVEDVQLRSNPRNA--RDPRAQVDDEDIA 69
           I +++VGK + + ++  IDEY  +L+ YC  N+ +V     P NA  ++     + E   
Sbjct: 3   ISIISVGKIKEKFLKAAIDEYSKRLSKYCKLNIIEVADEKTPDNASLKEENIIKEKEGNL 62

Query: 70  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 129
           ++  +K + +V+ LD  GK I+SE+ ++L+ +   TG S +AF IGG  G  QQ+  RAN
Sbjct: 63  ILKHIKDNSFVIALDLKGKSITSEEFSDLIENCRLTGNSTIAFVIGGSLGLSQQVLSRAN 122

Query: 130 VSIKLSSMVLNHQIALLVLMEQLYRGF 153
             +  S M   HQ+  ++L+EQ+YR F
Sbjct: 123 YKLSFSKMTFPHQLFRVMLLEQVYRAF 149

[Close]

BLAST of Carg21159 vs. ExPASy TrEMBL
Match: A0A6J1FPF2 (putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111446064 PE=3 SV=1)

HSP 1 Score: 283.1 bits (723), Expect = 1.1e-72
Identity = 147/155 (94.84%), Postives = 148/155 (95.48%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

[Close]

BLAST of Carg21159 vs. ExPASy TrEMBL
Match: A0A6J1IWR3 (putative RNA methyltransferase At5g10620 OS=Cucurbita maxima OX=3661 GN=LOC111481296 PE=3 SV=1)

HSP 1 Score: 276.2 bits (705), Expect = 1.3e-70
Identity = 143/155 (92.26%), Postives = 146/155 (94.19%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRN RD RAQVDDEDIA
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNTRDARAQVDDEDIA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           VSIKLSSMVLNHQIA+LVLMEQLYR +     Q Y
Sbjct: 146 VSIKLSSMVLNHQIAVLVLMEQLYRSWTILKGQKY 180

[Close]

BLAST of Carg21159 vs. ExPASy TrEMBL
Match: A0A1S3CQZ8 (putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770 PE=3 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 3.3e-69
Identity = 137/155 (88.39%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQLL+DEYIDKL NYC+VEDVQLRSNPRNARDP+AQ+DDED+A
Sbjct: 26  RAVPIRVLTVGKKRSRGVQLLVDEYIDKLKNYCHVEDVQLRSNPRNARDPKAQIDDEDVA 85

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRL+FCIGGPYGHGQ+LR+RAN
Sbjct: 86  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLSFCIGGPYGHGQKLRERAN 145

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           +SIKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 146 LSIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 180

[Close]

BLAST of Carg21159 vs. ExPASy TrEMBL
Match: A0A6J1E0Z2 (putative RNA methyltransferase At5g10620 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.8e-68
Identity = 136/155 (87.74%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQL++DEYI+KL +YCNVEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 32  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 91

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKE+SSE+MAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 92  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 151

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           ++IKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 152 LTIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 186

[Close]

BLAST of Carg21159 vs. ExPASy TrEMBL
Match: A0A6J1BPR4 (putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111026123 PE=3 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 2.8e-68
Identity = 136/155 (87.74%), Postives = 147/155 (94.84%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RAVPIRVLTVGKKRSRGVQL++DEYI+KL +YCNVEDVQLRSNPRNARDPRAQVDDED+A
Sbjct: 30  RAVPIRVLTVGKKRSRGVQLVVDEYIEKLKHYCNVEDVQLRSNPRNARDPRAQVDDEDMA 89

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           VVNLLKSDDWVVMLDENGKE+SSE+MAELVADAGNTGASRL+FCIGGPYGHGQQLRQRAN
Sbjct: 90  VVNLLKSDDWVVMLDENGKEMSSERMAELVADAGNTGASRLSFCIGGPYGHGQQLRQRAN 149

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           ++IKLSSMVLNHQIALLVLMEQLYR +     Q Y
Sbjct: 150 LTIKLSSMVLNHQIALLVLMEQLYRSWTILKGQKY 184

[Close]

BLAST of Carg21159 vs. TAIR 10
Match: AT5G10620.1 (methyltransferases )

HSP 1 Score: 214.2 bits (544), Expect = 1.2e-55
Identity = 106/155 (68.39%), Postives = 132/155 (85.16%), Query Frame = 0


Query: 6   RAVPIRVLTVGKKRSRGVQLLIDEYIDKLNNYCNVEDVQLRSNPRNARDPRAQVDDEDIA 65
           RA+PIRV+TVGKKRS GV+LL+DEY  KL  YC+ ED  +RSNPRNA+D RAQV+DE++A
Sbjct: 37  RALPIRVITVGKKRSEGVRLLVDEYKIKLKPYCSFEDSLVRSNPRNAQDVRAQVEDEEVA 96

Query: 66  VVNLLKSDDWVVMLDENGKEISSEQMAELVADAGNTGASRLAFCIGGPYGHGQQLRQRAN 125
           ++ L+ SDDWVV+LDE G++I SEQMAEL+ DAGN+GASR++FCIGG YGHG Q+R+RAN
Sbjct: 97  MMKLIGSDDWVVVLDERGRDIDSEQMAELLGDAGNSGASRISFCIGGAYGHGTQVRKRAN 156

Query: 126 VSIKLSSMVLNHQIALLVLMEQLYRGFLCHHHQTY 161
           V+I+LSSMVLNHQIAL+VLMEQLYR +     Q Y
Sbjct: 157 VTIRLSSMVLNHQIALVVLMEQLYRSWTILKGQNY 191

[Close]

BLAST of Carg21159 vs. TAIR 10
Match: AT5G10605.1 (methyltransferases )

HSP 1 Score: 57.4 bits (137), Expect = 1.9e-08
Identity = 28/36 (77.78%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 111 GGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLME 147
           G  YGHG+QLR+RANV+I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

[Close]

BLAST of Carg21159 vs. TAIR 10
Match: AT5G10605.2 (methyltransferases )

HSP 1 Score: 57.4 bits (137), Expect = 1.9e-08
Identity = 28/36 (77.78%), Postives = 33/36 (91.67%), Query Frame = 0


Query: 111 GGPYGHGQQLRQRANVSIKLSSMVLNHQIALLVLME 147
           G  YGHG+QLR+RANV+I+LSSMVLNH IAL+VLME
Sbjct: 21  GVAYGHGRQLRKRANVAIRLSSMVLNHHIALVVLME 56

The following BLAST results are available for this feature:

BLAST of Carg21159 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037551.1	2.1e-134	100.00	putative RNA methyltransferase [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022940478.1	2.2e-72	94.84	putative RNA methyltransferase At5g10620 [Cucurbita moschata] >KAG6608193.1 puta...	[more]
XP_023524400.1	7.2e-71	92.90	putative RNA methyltransferase At5g10620 [Cucurbita pepo subsp. pepo]	[more]
XP_022982492.1	2.7e-70	92.26	putative RNA methyltransferase At5g10620 [Cucurbita maxima] >XP_022982493.1 puta...	[more]
XP_038896820.1	6.1e-70	88.46	putative RNA methyltransferase At5g10620 isoform X1 [Benincasa hispida]	[more]

BLAST of Carg21159 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LXB4	1.7e-54	68.39	Putative RNA methyltransferase At5g10620 OS=Arabidopsis thaliana OX=3702 GN=At5g...	[more]
A0M0D0	8.7e-19	35.90	Ribosomal RNA large subunit methyltransferase H OS=Gramella forsetii (strain KT0...	[more]
A0PXE8	4.3e-18	34.84	Ribosomal RNA large subunit methyltransferase H OS=Clostridium novyi (strain NT)...	[more]
B2V1Q9	1.3e-17	34.19	Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain...	[more]
A7FZD1	3.7e-17	34.69	Ribosomal RNA large subunit methyltransferase H OS=Clostridium botulinum (strain...	[more]

BLAST of Carg21159 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FPF2	1.1e-72	94.84	putative RNA methyltransferase At5g10620 OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1IWR3	1.3e-70	92.26	putative RNA methyltransferase At5g10620 OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]
A0A1S3CQZ8	3.3e-69	88.39	putative RNA methyltransferase At5g10620 OS=Cucumis melo OX=3656 GN=LOC103503770...	[more]
A0A6J1E0Z2	2.8e-68	87.74	putative RNA methyltransferase At5g10620 isoform X1 OS=Momordica charantia OX=36...	[more]
A0A6J1BPR4	2.8e-68	87.74	putative RNA methyltransferase At5g10620 isoform X2 OS=Momordica charantia OX=36...	[more]

BLAST of Carg21159 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G10620.1	1.2e-55	68.39	methyltransferases	[more]
AT5G10605.1	1.9e-08	77.78	methyltransferases	[more]
AT5G10605.2	1.9e-08	77.78	methyltransferases	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 7..164 e-value: 1.0E-42 score: 147.2
IPR003742	RNA methyltransferase RlmH	PFAM	PF02590	SPOUT_MTase	coord: 10..160 e-value: 1.2E-40 score: 139.1
IPR003742	RNA methyltransferase RlmH	PANTHER	PTHR33603	METHYLTRANSFERASE	coord: 5..160
IPR003742	RNA methyltransferase RlmH	HAMAP	MF_00658	23SrRNA_methyltr_H	coord: 8..162 score: 20.449217
IPR003742	RNA methyltransferase RlmH	CDD	cd18081	RlmH-like	coord: 10..152 e-value: 1.72013E-53 score: 167.619
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 10..160

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21159-RA	Carg21159-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
biological_process	GO:0006364	rRNA processing
molecular_function	GO:0008168	methyltransferase activity