Carg21156 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21156
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	replication protein A 32 kDa subunit A-like
Location	Carg_Chr01: 10967336 .. 10971468 (+)
RNA-Seq Expression	Carg21156
Synteny	Carg21156

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTCCATTGTTGATTGTTCATTAAGTTTTTACTCGCTGAAGTTTGAAGAATGTTCTCCAGCAGCCAGTTTGATTCCGGCAGTGGATTTACGTCGTCTCAGACCAATGACTCCTCTTTGTCGAAGGTAAGTCGCTGCTTTAAACTCTAAATTTCTTCTCGATCGAGAGCGAATTTCAAAAATGCAGTATAGAATTTACTCTCCTAAAAGCTGTTGGATAGCTAGAATCCGGCGAAAACGCGAACCTAATTCATTTTTCATTCGCTGGAAGAGAGAATATCAGAGTATATTTAGGTGCGTTCAGAGCAGAGTTATAATTCTAGAAATGGAGATCAAACTCATAATGGTTATCCTATTTAATATTCGGTCGTAAACAAGATTTTGCTTGTCAAATTGGCGGTATTGAATAAAAATGAGAAGAATCTGTGAACAAGATGGTACTTGATAACTGTTCGTTTCATTTTCCACCATTTTCCTTTTATGTATCTTTGAATTCTTAATGCAGAGTCGAGAAACGCCGGGCGTGGTTCCAGTAACGGTAAAACAGATAAGTGAAGCCTCCCATCCTGGCGAAGAGAAGGCCAACTTTGTGATCAACGGCGTCGATATTACAAATGTATGCTTCCTTGCTTTCTCTCTCACTTTTAGAAACTACTGTCGCTGTGTATGTTTATTAGTGTAGCAAACAGATCATAGGATGAGAAATTGCAGTTCAGAATCAAATCTATGTTAATTATATCTGTCAATGTTGCTTACTAGCAATGCTTCAATGTTCGTCAGAGTTTTCAAAATTTTCATTGATTAGAATCCAACTACTAAGAAAAACATGATTTATTTGCCCACTCCCAAATTCTTGAATTGTGAGGAGGAGAACGAAAATCTTTATAAGGGTGTGGAAATCTAGCAACGAAATTTTTTATAAGGTTGTGGAAACCTCTGCTAGATGCATTTTAAAAACTTTGAGGGGAAGCCCGAAAGGAAAAGCTCAAAGAGGACAATATGTGCTAGCGGCAGGTTTAGTCGCTGGTTGGATTTCTGTACCTTTTAACCCAAAGAAGCATGAACATTAGCAGAGTGTTGAATGTTGCAATGCAAAGGGAAGGTTGTATATTTTAGTTTTACAAATTTTATTAATCTGAAAGCTTTGCAGTGTGAAAAATTATCAAACCATATTTTTCAGAAGTAAATGGTTATGTGTAAGTAACATATAAACTTTCAGTTCTTCATGAAAAATATCATTTCTTATAAGACCATGTCTGGCTCCTTGAGACTTGATCCTTTGTGTATGTATGTATTTCTTGTAGTCTGTAGTTCTTGAGTTTCTACAGACCCTTTTTCATTTCATATGTGGTTACCATTCACCTTAATTCGGGTCCATTTTATAGCTCGAGGATGTCTCTTGAATATTTGTAAATTCTCTTTGTTGTAAATACTGATATTTTACTTATTGGAAAAAGGTTACAATCGTCGGGAAGGTCTCTGAGAAAGCTGAAAGGAATACTGACATCACATTCATTGTAGATGATGGAACAGGAAGAATTGGCTGCAAAAGATGGTATACTGTTGCAGTTCCAATCTTCCTAAGCCCTTGAACTAAATCTGCTTCATTACTAAAGAATGTTTGTCAATATTTATTCAGGGTAAACGATACTTTTGACACTAAGCAAATGGATGAAATACAGTAAGTTCTTGTCCAATTTGCTTCTCTTGTGATGATAAATACTTAAGTAGTCTTTCTGACCCTTTCTTGGAATCTTTTATGGCATAGAGATGGAATGTATGTACGGGTAATTGGGCATCTGAAAATATTTCAAAGTAACAAGCAAATATTTGCATTCTCTGTAAGGTATGGTCTTTTAATTCTCTTAAGTCTAATTTCATGCGTTTGGGCATGCTTTTGAAGTTAAATGGTTAAGGCTATCATCCTGCTTGGTGATTTTATTGCATGCTGAAATTCGAGGATCTCCACATCAAATGAGTTCTAGTGATAATATCTTGTTAGTGTTATGTAGTTTCTCTTTGTGAATTTGTGATTGCCTAAAGTTTCTTCTTGAATCTACTGTCCTATGAAGTGTTCTTTCTTTCACCCTCGGTCACTAGGTAGTTATTGTATATTGGTAGTGACCAATCTCAAGTGATTATTGGGATGTTGGAAACCGTTCAATGCCTTTTTTTTTCCGGTTGTATATTTGATCAACTAACGGGATGATGAATTTGGCTATGGTTTTTCTTTTGCAGGCCTGTTACAAATTTTGACGAGATTACCTTCCACTTTATTGAATGCATACATGACCACTTGCGGAATTCCAAGTTACAGGTACTGATTGCTGGAGCCGACGTTGATAGTTCTGCTTCTAACTTCTTTTTAGTTATAAGTGAATATTAGAATTCTAAATTCATTTGCGCTGAAACTGCTTGAAGCCCAATGGAGATGTATCGACACAGTTCCAATCCTCGGATTCATCTGTGAAGCCTGCAGTGCAGAGTGGCTCGAGTGGATATCAGACAGCATCTTCAGGACTAGTAAGATTCTTAACCATGATTGTGACCGTGCTTTAATATGTAACCGCCTAAGCCCACCGCTAACAGATATTGTCTGTTTTAGCCCGTTACATATAGCCATCAGTCTCACGATTCTAAAACGCATCTGCTAGGGAGAGGTTCCACACCCTTATAAGAAATGTTTCATTCTCCTCTCCAACCAATATGAGATCTCACAATCCATCCCATTAGGGTTCCAGCGTTCTCACTGGCACACTGCCCGGTGTCTAGCCCTAATACAATTTGTAACAGCTCAAGCCCACCGCTAGCAGATATTGTTCATTTTGGCTTGTTATGTATCGCCGTCAACTTCACGGTTTTAAAACACGCCTACTAGGGAGAGGTTTCCACACCCTCATAAAAAATCATTTGTTCTTCTTTCCAACTAACGTGGATCTCATATAATATTTTGTAGATGATTATATAGTTAGATTGTGATTTAATAGCTTCTTTCTGCAGTCGGCTGAGCAGCACACTTTGATGTCAAAAAGAACTGTGACGAATTGGTCCTTGACTATCTGCAGCTGCCCTCCAGCGTGTAAGTTGTCCCAGACCATTTTTTCTTTTCCTTTTTGGATTGTCTCCTTTGTTTGCCAGTTGCTGTGCATTATTCTATATGAGGTTGACTTGAGTTGCCTTTGGCCCGGGAACAAGGTGGTTTTAGAATTAAAGAATTGAATTCCATGGTTGGATGTGACTGAGTCATTCATGGTCTGCTAGTTATAACTTGTGTGGATAGAAGACCAGACATTAGGTCGGTGGTTGACTGTTGTGTTAGACACAGATCACTTCTGATTATGGGTTCCATAGGACACTCACTCTTAGTTGGCTCTTGGCACAATAATAATAAGGAGACAAAGTTTGACTTTAGGGTCGCAATATTCAAGTTTCTTGTGGGCCACATTCATTGGAATCTTGCAAATATTTTTAACCAAGTTCGAGTTTAGTAGTTGTCTCGTGAGTTCAACCGAAAGAATGCACATATTTATCTTGTCTAAGTACACAAGTGAGATCATTTGATTATTGATAGTTTAGTTTCTTATCAAATAATCACTTTTAATCTATCTTATTAGTTATTAACATTCTCTTTTATGGCATGGAAACTATTATTATTATTTAATCAATTTCAATAAAAGTTAGTTTTGATAGTCTAATTTTATGATATCCATAAGTACATAGACTAATATAGAATAAAATTTATTGTACGGGTACCAATATTGTATTTTAACCAAAGAAAAATTAGAAAAAGTATTCTTTTCTCCCAAGTTTAAGAAAAAATAAATGGTTCATTACATTTGTATACATGTATAGAAGATATGTGTTTTTTATTTGAAGTTTTTAACTATATTTTGCAGAGCAAAAGAAAGAGGAATACACAGAGATGAACTGTCCCAACAACTGAAAATTCCTTTGAAGAAAATAGTGTAAACTTCTTCTATCCTCATCCAATGTGGTTGGTATGAAAATCAATTAAATTATACAACTTTTGTTTTCTTTTTCTTTTCTTTCAGGGACTCCATAAGGGCTCTTGAAGATGAAGGTTTGATATATTCCACCATAGATGAGTATCATTTCAAATCTGCTGCTGCATAG

mRNA sequence

CTCTCCATTGTTGATTGTTCATTAAGTTTTTACTCGCTGAAGTTTGAAGAATGTTCTCCAGCAGCCAGTTTGATTCCGGCAGTGGATTTACGTCGTCTCAGACCAATGACTCCTCTTTGTCGAAGAGTCGAGAAACGCCGGGCGTGGTTCCAGTAACGGTAAAACAGATAAGTGAAGCCTCCCATCCTGGCGAAGAGAAGGCCAACTTTGTGATCAACGGCGTCGATATTACAAATGTTACAATCGTCGGGAAGGTCTCTGAGAAAGCTGAAAGGAATACTGACATCACATTCATTGTAGATGATGGAACAGGAAGAATTGGCTGCAAAAGATGGGTAAACGATACTTTTGACACTAAGCAAATGGATGAAATACAAGATGGAATGTATGTACGGGTAATTGGGCATCTGAAAATATTTCAAAGTAACAAGCAAATATTTGCATTCTCTGTAAGGCCTGTTACAAATTTTGACGAGATTACCTTCCACTTTATTGAATGCATACATGACCACTTGCGGAATTCCAAGTTACAGGTACTGATTGCTGGAGCCGACGTTGATAGTTCTGCTTCTAACTTCTTTTTATTTTTAACTATATTTTGCAGAGCAAAAGAAAGAGGAATACACAGAGATGAACTGTCCCAACAACTGAAAATTCCTTTGAAGAAAATAGTGGACTCCATAAGGGCTCTTGAAGATGAAGGTTTGATATATTCCACCATAGATGAGTATCATTTCAAATCTGCTGCTGCATAG

Coding sequence (CDS)

ATGTTCTCCAGCAGCCAGTTTGATTCCGGCAGTGGATTTACGTCGTCTCAGACCAATGACTCCTCTTTGTCGAAGAGTCGAGAAACGCCGGGCGTGGTTCCAGTAACGGTAAAACAGATAAGTGAAGCCTCCCATCCTGGCGAAGAGAAGGCCAACTTTGTGATCAACGGCGTCGATATTACAAATGTTACAATCGTCGGGAAGGTCTCTGAGAAAGCTGAAAGGAATACTGACATCACATTCATTGTAGATGATGGAACAGGAAGAATTGGCTGCAAAAGATGGGTAAACGATACTTTTGACACTAAGCAAATGGATGAAATACAAGATGGAATGTATGTACGGGTAATTGGGCATCTGAAAATATTTCAAAGTAACAAGCAAATATTTGCATTCTCTGTAAGGCCTGTTACAAATTTTGACGAGATTACCTTCCACTTTATTGAATGCATACATGACCACTTGCGGAATTCCAAGTTACAGGTACTGATTGCTGGAGCCGACGTTGATAGTTCTGCTTCTAACTTCTTTTTATTTTTAACTATATTTTGCAGAGCAAAAGAAAGAGGAATACACAGAGATGAACTGTCCCAACAACTGAAAATTCCTTTGAAGAAAATAGTGGACTCCATAAGGGCTCTTGAAGATGAAGGTTTGATATATTCCACCATAGATGAGTATCATTTCAAATCTGCTGCTGCATAG

Protein sequence

MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDITNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADVDSSASNFFLFLTIFCRAKERGIHRDELSQQLKIPLKKIVDSIRALEDEGLIYSTIDEYHFKSAAA

Homology

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BLAST of Carg21156 vs. NCBI nr
Match: KAG7037548.1 (Replication protein A 32 kDa subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 460.7 bits (1184), Expect = 7.6e-126
Identity = 234/234 (100.00%), Postives = 234/234 (100.00%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADVDSSASNFFLFL 180
           KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADVDSSASNFFLFL
Sbjct: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADVDSSASNFFLFL 180

Query: 181 TIFCRAKERGIHRDELSQQLKIPLKKIVDSIRALEDEGLIYSTIDEYHFKSAAA 235
           TIFCRAKERGIHRDELSQQLKIPLKKIVDSIRALEDEGLIYSTIDEYHFKSAAA
Sbjct: 181 TIFCRAKERGIHRDELSQQLKIPLKKIVDSIRALEDEGLIYSTIDEYHFKSAAA 234

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BLAST of Carg21156 vs. NCBI nr
Match: XP_023524340.1 (replication protein A 32 kDa subunit A-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 386.0 bits (990), Expect = 2.4e-103
Identity = 212/271 (78.23%), Postives = 220/271 (81.18%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASH GEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQ------------------V 180
           KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQ                   
Sbjct: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQPNGDVSTQFQSSDSSVKPA 180

Query: 181 LIAGA-----------------DVDSSASNFFL-FLTI-FCRAKERGIHRDELSQQLKIP 235
           + +G+                 DV  +     L +L +    AKERGIHRDELSQQLKIP
Sbjct: 181 VQSGSSGYQTASSGLSSQQHTVDVKKNCDELVLDYLQLPSSVAKERGIHRDELSQQLKIP 240

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BLAST of Carg21156 vs. NCBI nr
Match: XP_022936562.1 (replication protein A 32 kDa subunit A-like [Cucurbita moschata])

HSP 1 Score: 368.2 bits (944), Expect = 5.1e-98
Identity = 196/271 (72.32%), Postives = 214/271 (78.97%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFTSSQTNDSS +K+RETPG+VP TVKQISEASH GEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMY+RV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-------------IAGA 180
           KIFQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQ L             I   
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 DVDSSASNFFLFLTIF-------------------------CRAKERGIHRDELSQQLKI 234
            + + +S +    ++                            AKERGIHRDELSQQLKI
Sbjct: 181 PMQNGSSGYQTASSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQLKI 240

[Close]

BLAST of Carg21156 vs. NCBI nr
Match: KAG7024504.1 (Replication protein A 32 kDa subunit A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 363.6 bits (932), Expect = 1.3e-96
Identity = 194/271 (71.59%), Postives = 212/271 (78.23%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFTSSQT DSS +K+RETPG+VP TVKQISEASH GEEKANF INGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTYDSSSAKNRETPGLVPATVKQISEASHSGEEKANFAINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMY+RV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-------------IAGA 180
           KIFQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQ L             I   
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 DVDSSASNFFLFLTIF-------------------------CRAKERGIHRDELSQQLKI 234
            + + +S +    ++                            AKERGIHRDELSQQLKI
Sbjct: 181 PMQNGSSGYQTASSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQLKI 240

[Close]

BLAST of Carg21156 vs. NCBI nr
Match: XP_022976184.1 (replication protein A 32 kDa subunit A-like [Cucurbita maxima])

HSP 1 Score: 363.2 bits (931), Expect = 1.7e-96
Identity = 194/273 (71.06%), Postives = 212/273 (77.66%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFT SQTNDSS +K+RETPG+VP TVKQISEASH GEEKANF INGVDI
Sbjct: 1   MFSSSQFDSGSGFTFSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFAINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMY+RV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-------------IAGA 180
           KIFQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQ L             I   
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 DVDSSASNFFLFLTIFCR---------------------------AKERGIHRDELSQQL 234
            + + +S +    ++                              AKERGIHRDELSQQL
Sbjct: 181 PMQNGSSGYQTASSVSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQL 240

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BLAST of Carg21156 vs. ExPASy Swiss-Prot
Match: Q9ZQ19 (Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A PE=1 SV=2)

HSP 1 Score: 219.9 bits (559), Expect = 3.0e-56
Identity = 125/276 (45.29%), Postives = 171/276 (61.96%), Query Frame = 0


Query: 1   MFSSSQFD-----SGSGFTSSQ-----TNDSSLSKSRETPGVVPVTVKQISEASHPGEEK 60
           MFSSSQF+     SG GF SSQ      + SS +K+R+  G+VPVTVKQI+E      EK
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  ANFVINGVDITNVTIVGKVSEKAE-RNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQ 120
           +  VING+ +TNV++VG V +K E + T++ F +DDGTGRI CKRWV++TFD ++M+ ++
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVIGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADV 180
           DG YVR+ GHLK FQ   Q+  FSVRP+ +F+E+TFH+IECIH + +NS+ Q    G DV
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVG-DV 180

Query: 181 DSSASNFF-------------------------------LFLTIF----CRAKERGIHRD 231
             S +  F                               + L       C A+++GIH D
Sbjct: 181 TQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQGIHID 240

[Close]

BLAST of Carg21156 vs. ExPASy Swiss-Prot
Match: Q6K9U2 (Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2A PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 4.4e-44
Identity = 116/276 (42.03%), Postives = 157/276 (56.88%), Query Frame = 0


Query: 1   MFSSSQFD--SGSGFTSSQ---TNDSSLSKSRETPGVVPVTVKQISEASH---PGEEKAN 60
           M S SQ D  S S FTSSQ    + ++ SKSR     +P+TVKQISEA      GE+ A 
Sbjct: 1   MMSFSQPDAFSPSQFTSSQNAAADSTTPSKSRGASSTMPLTVKQISEAQQSGITGEKGAP 60

Query: 61  FVINGVDITNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGM 120
           FV++GV+  NV +VG VS K ERNTD++F +DDGTGR+   RWVND  D+ +   +Q+GM
Sbjct: 61  FVVDGVETANVRLVGLVSGKTERNTDVSFTIDDGTGRLDFIRWVNDGADSAETAAVQNGM 120

Query: 121 YVRVIGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQV---LIAGADV 180
           YV VIG LK  Q  K+  AF++RPVT+++E+T HFI+C+  HL N+K Q+       + +
Sbjct: 121 YVSVIGSLKGLQERKRATAFAIRPVTDYNEVTLHFIQCVRMHLENTKSQIGSPAKTYSAM 180

Query: 181 DSSASNFFLFLTIFCRAK---------------------------------ERGIHRDEL 233
            SS+SN F  +T     K                                 E G+H DE+
Sbjct: 181 GSSSSNGFSEMTTPTSVKSNPAPVLSVTNGSKTDLNTEVLNVFREPANVESEHGVHIDEI 240

[Close]

BLAST of Carg21156 vs. ExPASy Swiss-Prot
Match: Q8LFJ8 (Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B PE=2 SV=1)

HSP 1 Score: 160.2 bits (404), Expect = 2.8e-38
Identity = 99/273 (36.26%), Postives = 149/273 (54.58%), Query Frame = 0


Query: 2   FSSSQFDSGSGFTSSQT-----NDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVIN 61
           F  +   +G GF  SQ        SS  K+R+   ++P+T+KQ+S AS  GE  +NF I+
Sbjct: 6   FDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSASTTGE--SNFSID 65

Query: 62  GVDITNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRV 121
           GVDI  V IVG++S    R T + F+VDDGTG + C RW +   +T++M+ ++ GMYVR+
Sbjct: 66  GVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRL 125

Query: 122 IGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQ--------------- 181
            GHLKIFQ  + +  FSVRPVT+F+EI  HF EC++ H+ N+KL+               
Sbjct: 126 HGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQM 185

Query: 182 ----VLIAGADVDSSASNFF-------------LFLTIFCR----AKERGIHRDELSQQL 234
               +        +  SN F               L    +      E G+H D ++++L
Sbjct: 186 PYSTMPTPAKPYQTGPSNQFPNQFNDSMHGVKQTVLNYLNQPMHIVSEAGVHCDIIAREL 245

[Close]

BLAST of Carg21156 vs. ExPASy Swiss-Prot
Match: Q6H7J5 (Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2B PE=1 SV=1)

HSP 1 Score: 154.8 bits (390), Expect = 1.2e-36
Identity = 89/275 (32.36%), Postives = 146/275 (53.09%), Query Frame = 0


Query: 3   SSSQFDSGSGFTSSQTNDSS---------LSKSRETPGVVPVTVKQISEASHPGEEKANF 62
           ++S    G GF  SQ  +++           KSR    ++P+TVKQI +AS   ++K+NF
Sbjct: 20  NASSLFGGGGFMPSQATNAAEGTSGGGGGFPKSRNAQALLPLTVKQIMDASQTNDDKSNF 79

Query: 63  VINGVDITNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMY 122
            +NG++++ V +VG++  K +R TD++F +DDGTGR+   RW ND+ DTK+M +IQ+G Y
Sbjct: 80  AVNGMEVSTVRLVGRMLNKLDRVTDVSFTLDDGTGRVPVNRWENDSTDTKEMADIQNGDY 139

Query: 123 VRVIGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLR----------------- 182
           V V G LK FQ  +Q+ A+SVR +TNF+++T HF+ C+H HL                  
Sbjct: 140 VIVNGGLKGFQGKRQVVAYSVRRITNFNDVTHHFLHCVHVHLELTRPKSQVNANTATGTP 199

Query: 183 ----------------NSKLQVLIAGADVDSSASNFFLFLTIF----CRAKERGIHRDEL 232
                            ++     A  +  +  +   L L +F        + G+  D +
Sbjct: 200 NQTMPRDSMAYNQSPLTNQASTFSAPQNTGTGTNMIDLVLNVFHDPAVMNDDHGVGVDYV 259

[Close]

BLAST of Carg21156 vs. ExPASy Swiss-Prot
Match: Q5Z8L1 (Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 GN=RPA2C PE=1 SV=2)

HSP 1 Score: 111.3 bits (277), Expect = 1.5e-23
Identity = 63/160 (39.38%), Postives = 92/160 (57.50%), Query Frame = 0


Query: 9   SGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEA-----SHPGEEKANFVINGVDITNV 68
           S +G  ++     S  +     G VP TV  IS +     +  G     F I+GV+ TNV
Sbjct: 27  SAAGTGAAAAPSPSKPRDPRFSGCVPATVLHISRSFAAALAADGGGDPVFSIDGVETTNV 86

Query: 69  TIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHLKIF 128
            ++G+V     R+TD+ F +DD TG+I   RW+ D  DT+    IQ+G+YV+V  +L  F
Sbjct: 87  RVLGRVVSVVSRDTDVCFTLDDSTGKIPLVRWITDQSDTRDTSYIQEGVYVKVQVNLMGF 146

Query: 129 QSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRN--SKLQ 162
           Q+ KQ  A S+RP+ NF+E+  HFIEC+H HL +  SK+Q
Sbjct: 147 QAKKQGLARSIRPINNFNEVVLHFIECMHVHLESVQSKMQ 186

[Close]

BLAST of Carg21156 vs. ExPASy TrEMBL
Match: A0A6J1F8S8 (replication protein A 32 kDa subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC111443128 PE=3 SV=1)

HSP 1 Score: 368.2 bits (944), Expect = 2.5e-98
Identity = 196/271 (72.32%), Postives = 214/271 (78.97%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFTSSQTNDSS +K+RETPG+VP TVKQISEASH GEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMY+RV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-------------IAGA 180
           KIFQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQ L             I   
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 DVDSSASNFFLFLTIF-------------------------CRAKERGIHRDELSQQLKI 234
            + + +S +    ++                            AKERGIHRDELSQQLKI
Sbjct: 181 PMQNGSSGYQTASSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQLKI 240

[Close]

BLAST of Carg21156 vs. ExPASy TrEMBL
Match: A0A6J1IMU1 (replication protein A 32 kDa subunit A-like OS=Cucurbita maxima OX=3661 GN=LOC111476648 PE=3 SV=1)

HSP 1 Score: 363.2 bits (931), Expect = 8.0e-97
Identity = 194/273 (71.06%), Postives = 212/273 (77.66%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFT SQTNDSS +K+RETPG+VP TVKQISEASH GEEKANF INGVDI
Sbjct: 1   MFSSSQFDSGSGFTFSQTNDSSSAKNRETPGLVPATVKQISEASHSGEEKANFAINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMY+RV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYIRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-------------IAGA 180
           KIFQSNKQIFAFSVRP+TNFDEITFHFIECIH+HLRNSKLQ L             I   
Sbjct: 121 KIFQSNKQIFAFSVRPITNFDEITFHFIECIHEHLRNSKLQNLNGVGSTQFQSSDSIVKT 180

Query: 181 DVDSSASNFFLFLTIFCR---------------------------AKERGIHRDELSQQL 234
            + + +S +    ++                              AKERGIHRDELSQQL
Sbjct: 181 PMQNGSSGYQTASSVSVLPSEQHTVDVKKNCDDLILDYLQLPSSVAKERGIHRDELSQQL 240

[Close]

BLAST of Carg21156 vs. ExPASy TrEMBL
Match: A0A1S3CR64 (replication protein A 32 kDa subunit A-like OS=Cucumis melo OX=3656 GN=LOC103503766 PE=3 SV=1)

HSP 1 Score: 362.5 bits (929), Expect = 1.4e-96
Identity = 195/271 (71.96%), Postives = 211/271 (77.86%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSS+QFDSGSGFTSSQTNDSS +KSRE+PG+VPVTVKQISEASH GEEKANFVIN VDI
Sbjct: 1   MFSSTQFDSGSGFTSSQTNDSSSAKSRESPGLVPVTVKQISEASHSGEEKANFVINDVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDTKQM+EIQDGMYVRV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTKQMEEIQDGMYVRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-------------IAGA 180
           K+FQSNKQIFAFSVRPVTNFDEITFHF+ECIHDHLRNSKLQ L             I   
Sbjct: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFLECIHDHLRNSKLQNLKGDGSTQLQTSDSIVKT 180

Query: 181 DVDSSASNFFLFLTIF-------------------------CRAKERGIHRDELSQQLKI 234
            V + +S +    +                             AKERGIHRDELSQQLKI
Sbjct: 181 PVQNGSSGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHRDELSQQLKI 240

[Close]

BLAST of Carg21156 vs. ExPASy TrEMBL
Match: A0A0A0LEG7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881710 PE=3 SV=1)

HSP 1 Score: 359.4 bits (921), Expect = 1.2e-95
Identity = 194/271 (71.59%), Postives = 210/271 (77.49%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSS+QFDS SGFTSSQTNDSS +KSRE+PG++PVTVKQISEASH GEEKANFVINGVDI
Sbjct: 1   MFSSTQFDSASGFTSSQTNDSSSAKSRESPGLIPVTVKQISEASHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITF VDDGTG IGCKRWVNDTFDT QM+EIQDGMYVRV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFTVDDGTGTIGCKRWVNDTFDTNQMEEIQDGMYVRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAG-------------- 180
           K+FQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQ L                 
Sbjct: 121 KMFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQNLKGNGSTQLQTSDSIVKT 180

Query: 181 ----------------------ADVDSSASNFFL-FLTI-FCRAKERGIHRDELSQQLKI 234
                                  DV  S     L +L +    AKERGIH+DELSQQLK+
Sbjct: 181 PVQNGSNGYHTTSSAIPSEQHTVDVKKSCDELVLDYLQLPSSVAKERGIHKDELSQQLKL 240

[Close]

BLAST of Carg21156 vs. ExPASy TrEMBL
Match: A0A6J1CUB8 (replication protein A 32 kDa subunit A OS=Momordica charantia OX=3673 GN=LOC111014622 PE=3 SV=1)

HSP 1 Score: 354.0 bits (907), Expect = 4.9e-94
Identity = 192/270 (71.11%), Postives = 210/270 (77.78%), Query Frame = 0


Query: 1   MFSSSQFDSGSGFTSSQTNDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVINGVDI 60
           MFSSSQFDSGSGFTSSQ  DS+ +KSRE+PG+VPVTVKQISEA H GEEKANFVINGVDI
Sbjct: 1   MFSSSQFDSGSGFTSSQVPDSTSAKSRESPGLVPVTVKQISEAFHSGEEKANFVINGVDI 60

Query: 61  TNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHL 120
           TNVTIVGKVSEKAERNTDITF+VDDGTGRIGCKRWVNDTFDTKQMD+IQDGMY+RV GHL
Sbjct: 61  TNVTIVGKVSEKAERNTDITFVVDDGTGRIGCKRWVNDTFDTKQMDDIQDGMYIRVNGHL 120

Query: 121 KIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVL-IAGADVDSSASNFFLF 180
           KIFQ N QIFAFSVRPVTN DEITFHFIECIHD+LRNSKLQ+  +A     SS S     
Sbjct: 121 KIFQGNMQIFAFSVRPVTNCDEITFHFIECIHDYLRNSKLQLNGVASTQSQSSDSTVNAL 180

Query: 181 L-------------------TIFCR-----------------AKERGIHRDELSQQLKIP 234
           +                   T+  +                 AKE+GIHRDELSQQLKIP
Sbjct: 181 VQSGSSGYQTASSELSSGQHTVDVKKNPDDLVLDYLQLPSSVAKEKGIHRDELSQQLKIP 240

[Close]

BLAST of Carg21156 vs. TAIR 10
Match: AT2G24490.1 (replicon protein A2 )

HSP 1 Score: 219.9 bits (559), Expect = 2.1e-57
Identity = 125/276 (45.29%), Postives = 171/276 (61.96%), Query Frame = 0


Query: 1   MFSSSQFD-----SGSGFTSSQ-----TNDSSLSKSRETPGVVPVTVKQISEASHPGEEK 60
           MFSSSQF+     SG GF SSQ      + SS +K+R+  G+VPVTVKQI+E      EK
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  ANFVINGVDITNVTIVGKVSEKAE-RNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQ 120
           +  VING+ +TNV++VG V +K E + T++ F +DDGTGRI CKRWV++TFD ++M+ ++
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVIGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADV 180
           DG YVR+ GHLK FQ   Q+  FSVRP+ +F+E+TFH+IECIH + +NS+ Q    G DV
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVG-DV 180

Query: 181 DSSASNFF-------------------------------LFLTIF----CRAKERGIHRD 231
             S +  F                               + L       C A+++GIH D
Sbjct: 181 TQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQGIHID 240

[Close]

BLAST of Carg21156 vs. TAIR 10
Match: AT2G24490.2 (replicon protein A2 )

HSP 1 Score: 219.9 bits (559), Expect = 2.1e-57
Identity = 125/276 (45.29%), Postives = 171/276 (61.96%), Query Frame = 0


Query: 1   MFSSSQFD-----SGSGFTSSQ-----TNDSSLSKSRETPGVVPVTVKQISEASHPGEEK 60
           MFSSSQF+     SG GF SSQ      + SS +K+R+  G+VPVTVKQI+E      EK
Sbjct: 1   MFSSSQFEPNSGFSGGGFMSSQPSQAYESSSSTAKNRDFQGLVPVTVKQITECFQSSGEK 60

Query: 61  ANFVINGVDITNVTIVGKVSEKAE-RNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQ 120
           +  VING+ +TNV++VG V +K E + T++ F +DDGTGRI CKRWV++TFD ++M+ ++
Sbjct: 61  SGLVINGISLTNVSLVGLVCDKDESKVTEVRFTLDDGTGRIDCKRWVSETFDAREMESVR 120

Query: 121 DGMYVRVIGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQVLIAGADV 180
           DG YVR+ GHLK FQ   Q+  FSVRP+ +F+E+TFH+IECIH + +NS+ Q    G DV
Sbjct: 121 DGTYVRLSGHLKTFQGKTQLLVFSVRPIMDFNEVTFHYIECIHFYSQNSESQRQQVG-DV 180

Query: 181 DSSASNFF-------------------------------LFLTIF----CRAKERGIHRD 231
             S +  F                               + L       C A+++GIH D
Sbjct: 181 TQSVNTTFQGGSNTNQATLLNPVVSSQNNDGNGRKNLDDMILDYLKQPACTARQQGIHID 240

[Close]

BLAST of Carg21156 vs. TAIR 10
Match: AT3G02920.1 (Replication protein A, subunit RPA32 )

HSP 1 Score: 160.2 bits (404), Expect = 2.0e-39
Identity = 99/273 (36.26%), Postives = 149/273 (54.58%), Query Frame = 0


Query: 2   FSSSQFDSGSGFTSSQT-----NDSSLSKSRETPGVVPVTVKQISEASHPGEEKANFVIN 61
           F  +   +G GF  SQ        SS  K+R+   ++P+T+KQ+S AS  GE  +NF I+
Sbjct: 6   FDGNAAFAGGGFMPSQATTQAHESSSSLKNRDVRTLLPLTLKQLSSASTTGE--SNFSID 65

Query: 62  GVDITNVTIVGKVSEKAERNTDITFIVDDGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRV 121
           GVDI  V IVG++S    R T + F+VDDGTG + C RW +   +T++M+ ++ GMYVR+
Sbjct: 66  GVDIKTVVIVGRISRMENRITQVDFVVDDGTGWVDCVRWCHARQETEEMEAVKLGMYVRL 125

Query: 122 IGHLKIFQSNKQIFAFSVRPVTNFDEITFHFIECIHDHLRNSKLQ--------------- 181
            GHLKIFQ  + +  FSVRPVT+F+EI  HF EC++ H+ N+KL+               
Sbjct: 126 HGHLKIFQGKRSVNVFSVRPVTDFNEIVHHFTECMYVHMYNTKLRGGSITQDTATPRPQM 185

Query: 182 ----VLIAGADVDSSASNFF-------------LFLTIFCR----AKERGIHRDELSQQL 234
               +        +  SN F               L    +      E G+H D ++++L
Sbjct: 186 PYSTMPTPAKPYQTGPSNQFPNQFNDSMHGVKQTVLNYLNQPMHIVSEAGVHCDIIAREL 245

[Close]

BLAST of Carg21156 vs. TAIR 10
Match: AT3G02920.2 (Replication protein A, subunit RPA32 )

HSP 1 Score: 158.7 bits (400), Expect = 5.8e-39
Identity = 90/245 (36.73%), Postives = 139/245 (56.73%), Query Frame = 0


Query: 25  KSRETPGVVPVTVKQISEASHPGEEKANFVINGVDITNVTIVGKVSEKAERNTDITFIVD 84
           ++R+   ++P+T+KQ+S AS  GE  +NF I+GVDI  V IVG++S    R T + F+VD
Sbjct: 54  RNRDVRTLLPLTLKQLSSASTTGE--SNFSIDGVDIKTVVIVGRISRMENRITQVDFVVD 113

Query: 85  DGTGRIGCKRWVNDTFDTKQMDEIQDGMYVRVIGHLKIFQSNKQIFAFSVRPVTNFDEIT 144
           DGTG + C RW +   +T++M+ ++ GMYVR+ GHLKIFQ  + +  FSVRPVT+F+EI 
Sbjct: 114 DGTGWVDCVRWCHARQETEEMEAVKLGMYVRLHGHLKIFQGKRSVNVFSVRPVTDFNEIV 173

Query: 145 FHFIECIHDHLRNSKLQ-------------------VLIAGADVDSSASNFF-------- 204
            HF EC++ H+ N+KL+                   +        +  SN F        
Sbjct: 174 HHFTECMYVHMYNTKLRGGSITQDTATPRPQMPYSTMPTPAKPYQTGPSNQFPNQFNDSM 233

Query: 205 -----LFLTIFCR----AKERGIHRDELSQQLKIPLKKIVDSIRALEDEGLIYSTIDEYH 234
                  L    +      E G+H D ++++L+IPL ++ +++  L ++G IYST+DE  
Sbjct: 234 HGVKQTVLNYLNQPMHIVSEAGVHCDIIARELRIPLLQVKEALEQLSNDGCIYSTLDETC 293

The following BLAST results are available for this feature:

BLAST of Carg21156 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7037548.1	7.6e-126	100.00	Replication protein A 32 kDa subunit A [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_023524340.1	2.4e-103	78.23	replication protein A 32 kDa subunit A-like [Cucurbita pepo subsp. pepo]	[more]
XP_022936562.1	5.1e-98	72.32	replication protein A 32 kDa subunit A-like [Cucurbita moschata]	[more]
KAG7024504.1	1.3e-96	71.59	Replication protein A 32 kDa subunit A [Cucurbita argyrosperma subsp. argyrosper...	[more]
XP_022976184.1	1.7e-96	71.06	replication protein A 32 kDa subunit A-like [Cucurbita maxima]	[more]

BLAST of Carg21156 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZQ19	3.0e-56	45.29	Replication protein A 32 kDa subunit A OS=Arabidopsis thaliana OX=3702 GN=RPA2A ...	[more]
Q6K9U2	4.4e-44	42.03	Replication protein A 32 kDa subunit A OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q8LFJ8	2.8e-38	36.26	Replication protein A 32 kDa subunit B OS=Arabidopsis thaliana OX=3702 GN=RPA2B ...	[more]
Q6H7J5	1.2e-36	32.36	Replication protein A 32 kDa subunit B OS=Oryza sativa subsp. japonica OX=39947 ...	[more]
Q5Z8L1	1.5e-23	39.38	Replication protein A 32 kDa subunit C OS=Oryza sativa subsp. japonica OX=39947 ...	[more]

BLAST of Carg21156 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1F8S8	2.5e-98	72.32	replication protein A 32 kDa subunit A-like OS=Cucurbita moschata OX=3662 GN=LOC...	[more]
A0A6J1IMU1	8.0e-97	71.06	replication protein A 32 kDa subunit A-like OS=Cucurbita maxima OX=3661 GN=LOC11...	[more]
A0A1S3CR64	1.4e-96	71.96	replication protein A 32 kDa subunit A-like OS=Cucumis melo OX=3656 GN=LOC103503...	[more]
A0A0A0LEG7	1.2e-95	71.59	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G881710 PE=3 SV=1	[more]
A0A6J1CUB8	4.9e-94	71.11	replication protein A 32 kDa subunit A OS=Momordica charantia OX=3673 GN=LOC1110...	[more]

BLAST of Carg21156 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G24490.1	2.1e-57	45.29	replicon protein A2	[more]
AT2G24490.2	2.1e-57	45.29	replicon protein A2	[more]
AT3G02920.1	2.0e-39	36.26	Replication protein A, subunit RPA32	[more]
AT3G02920.2	5.8e-39	36.73	Replication protein A, subunit RPA32	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR014646	Replication factor A protein 2	PIRSF	PIRSF036949	RPA32	coord: 1..178 e-value: 7.6E-54 score: 180.5 coord: 176..233 e-value: 3.0E-10 score: 37.6
IPR036388	Winged helix-like DNA-binding domain superfamily	GENE3D	1.10.10.10		coord: 179..234 e-value: 8.8E-15 score: 56.2
IPR004365	OB-fold nucleic acid binding domain, AA-tRNA synthetase-type	PFAM	PF01336	tRNA_anti-codon	coord: 63..135 e-value: 6.7E-10 score: 38.8
None	No IPR available	GENE3D	2.40.50.140		coord: 29..160 e-value: 8.0E-43 score: 147.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..29
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..30
None	No IPR available	PANTHER	PTHR13989:SF34	REPLICATION PROTEIN A 32 KDA SUBUNIT A	coord: 185..232
None	No IPR available	PANTHER	PTHR13989:SF34	REPLICATION PROTEIN A 32 KDA SUBUNIT A	coord: 1..166
None	No IPR available	CDD	cd04478	RPA2_DBD_D	coord: 62..155 e-value: 2.13537E-40 score: 131.953
IPR014892	Replication protein A, C-terminal	PFAM	PF08784	RPA_C	coord: 186..226 e-value: 2.0E-9 score: 38.0
IPR040260	Replication factor A protein-like	PANTHER	PTHR13989	REPLICATION PROTEIN A-RELATED	coord: 185..232 coord: 1..166
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 32..158
IPR036390	Winged helix DNA-binding domain superfamily	SUPERFAMILY	46785	"Winged helix" DNA-binding domain	coord: 186..232

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21156-RA	Carg21156-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006260	DNA replication
biological_process	GO:0000724	double-strand break repair via homologous recombination
biological_process	GO:0006289	nucleotide-excision repair
biological_process	GO:0006310	DNA recombination
biological_process	GO:0006281	DNA repair
cellular_component	GO:0000781	chromosome, telomeric region
cellular_component	GO:0005662	DNA replication factor A complex
cellular_component	GO:0035861	site of double-strand break
cellular_component	GO:0005634	nucleus
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003697	single-stranded DNA binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0003676	nucleic acid binding