Carg21134 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21134
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	protein MIZU-KUSSEI 1
Location	Carg_Chr07: 7970498 .. 7971477 (-)
RNA-Seq Expression	Carg21134
Synteny	Carg21134

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGACAGGCCTGCAAAGAACCAAAAGCTGCAGCGCCGCCATTAAAACCACTACCAAAATCATCCCATCTTCCATCTCCGCCCACCACCTCAGATCGTCCTCCGATGCTGACAACTTTTCAGGCAAATTCCTCCTCCAGAAGAACTCTAGCCACCGCTGTCACCGCCAACACCTGGCGGCTTCCACCACCGGGAAATTCTCCTTCCTCCTTCACTCTCTTCTCAAGCTACTCTCAATTCCCACCTGCAAATGGCTTTCCATCCCCACCCACCTCTCCGTCAGCCCCTCTCTCGGCCGAAGAGTCACTGGAACTCTCTTCGGCCGCCGCCGTGGCCATGTCACCTTCGCCATCCAACTCGACCCCCGGTCCCAGCCAGTTTTGTTGCTCGAATTCGCCACGTCAACCTCGTCCCTCATCAAGGAAATGTCCTCCGGTCTCGTTCGCATCGCCCTCGAGTCCGATGCTCCGACCACCTTCGACGGTATTACCATAATTTTGAAATTTTAATACGTTTAACAATCGAAAACACTCGTACTCTTTGTTAAGATTAATTCACATTTTTTTAATTAAAATACATAAATAATTTTTTAATAAATAAATATAATTATTAGATTTGACTTCTTGATTGTATTAGTAATGATATTTTAACATTAATAGGTAATCGTCCCATTAGAAAGTTGTTGGAGGAGCCAAGGTGGACGATGTACTGCAACGGGAGGAAGTGTGGACAGGCTATGTCACGCACGTGTGGAGAGTTTGAGTGGTACGTGCTGAACACTGTACAAAGTGTGTCAGTCGGCGCCGGAGTGATTCCAATGGTGGACGATGGTCGTAAAGGCAGTGGGTCAGAGGGCGAGTTGTTGTACATGAGAGCAAAGTTTGAGCGAGTTATAGGTAGCCGTGACTCGGAAGCTTTCTACATGATGAATCCAGATGGTAATGGAGGACCTGAACTCAGTATTTTTCTACTCCGAATATAA

mRNA sequence

ATGACAGGCCTGCAAAGAACCAAAAGCTGCAGCGCCGCCATTAAAACCACTACCAAAATCATCCCATCTTCCATCTCCGCCCACCACCTCAGATCGTCCTCCGATGCTGACAACTTTTCAGGCAAATTCCTCCTCCAGAAGAACTCTAGCCACCGCTGTCACCGCCAACACCTGGCGGCTTCCACCACCGGGAAATTCTCCTTCCTCCTTCACTCTCTTCTCAAGCTACTCTCAATTCCCACCTGCAAATGGCTTTCCATCCCCACCCACCTCTCCGTCAGCCCCTCTCTCGGCCGAAGAGTCACTGGAACTCTCTTCGGCCGCCGCCGTGGCCATGTCACCTTCGCCATCCAACTCGACCCCCGGTCCCAGCCAGTTTTGTTGCTCGAATTCGCCACGTCAACCTCGTCCCTCATCAAGGAAATGTCCTCCGGTCTCGTTCGCATCGCCCTCGAGTCCGATGCTCCGACCACCTTCGACGGTAATCGTCCCATTAGAAAGTTGTTGGAGGAGCCAAGGTGGACGATGTACTGCAACGGGAGGAAGTGTGGACAGGCTATGTCACGCACGTGTGGAGAGTTTGAGTGGTACGTGCTGAACACTGTACAAAGTGTGTCAGTCGGCGCCGGAGTGATTCCAATGGTGGACGATGGTCGTAAAGGCAGTGGGTCAGAGGGCGAGTTGTTGTACATGAGAGCAAAGTTTGAGCGAGTTATAGGTAGCCGTGACTCGGAAGCTTTCTACATGATGAATCCAGATGGTAATGGAGGACCTGAACTCAGTATTTTTCTACTCCGAATATAA

Coding sequence (CDS)

ATGACAGGCCTGCAAAGAACCAAAAGCTGCAGCGCCGCCATTAAAACCACTACCAAAATCATCCCATCTTCCATCTCCGCCCACCACCTCAGATCGTCCTCCGATGCTGACAACTTTTCAGGCAAATTCCTCCTCCAGAAGAACTCTAGCCACCGCTGTCACCGCCAACACCTGGCGGCTTCCACCACCGGGAAATTCTCCTTCCTCCTTCACTCTCTTCTCAAGCTACTCTCAATTCCCACCTGCAAATGGCTTTCCATCCCCACCCACCTCTCCGTCAGCCCCTCTCTCGGCCGAAGAGTCACTGGAACTCTCTTCGGCCGCCGCCGTGGCCATGTCACCTTCGCCATCCAACTCGACCCCCGGTCCCAGCCAGTTTTGTTGCTCGAATTCGCCACGTCAACCTCGTCCCTCATCAAGGAAATGTCCTCCGGTCTCGTTCGCATCGCCCTCGAGTCCGATGCTCCGACCACCTTCGACGGTAATCGTCCCATTAGAAAGTTGTTGGAGGAGCCAAGGTGGACGATGTACTGCAACGGGAGGAAGTGTGGACAGGCTATGTCACGCACGTGTGGAGAGTTTGAGTGGTACGTGCTGAACACTGTACAAAGTGTGTCAGTCGGCGCCGGAGTGATTCCAATGGTGGACGATGGTCGTAAAGGCAGTGGGTCAGAGGGCGAGTTGTTGTACATGAGAGCAAAGTTTGAGCGAGTTATAGGTAGCCGTGACTCGGAAGCTTTCTACATGATGAATCCAGATGGTAATGGAGGACCTGAACTCAGTATTTTTCTACTCCGAATATAA

Protein sequence

MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI

Homology

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BLAST of Carg21134 vs. NCBI nr
Match: KAG7027634.1 (Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 533.5 bits (1373), Expect = 1.1e-147
Identity = 267/267 (100.00%), Postives = 267/267 (100.00%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60
           MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60

Query: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120
           STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD
Sbjct: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120

Query: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180
           PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG
Sbjct: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180

Query: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240
           RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG
Sbjct: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240

Query: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 268
           SRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. NCBI nr
Match: XP_022924911.1 (protein MIZU-KUSSEI 1 [Cucurbita moschata])

HSP 1 Score: 528.9 bits (1361), Expect = 2.6e-146
Identity = 265/267 (99.25%), Postives = 266/267 (99.63%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60
           MTGLQRTKSCSAAI+TTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA
Sbjct: 1   MTGLQRTKSCSAAIQTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60

Query: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120
           STTGKFS LLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD
Sbjct: 61  STTGKFSSLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120

Query: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180
           PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG
Sbjct: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180

Query: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240
           RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG
Sbjct: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240

Query: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 268
           SRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. NCBI nr
Match: XP_023518638.1 (protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 518.8 bits (1335), Expect = 2.7e-143
Identity = 259/267 (97.00%), Postives = 264/267 (98.88%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60
           MTGLQRT+SCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA
Sbjct: 1   MTGLQRTRSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60

Query: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120
           STTGKFS LLHSLLKLLSIPTCKWL IPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD
Sbjct: 61  STTGKFSSLLHSLLKLLSIPTCKWLPIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120

Query: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180
           PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESD+PTT+DGNRPIRKLLEEPRWTMY NG
Sbjct: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDSPTTYDGNRPIRKLLEEPRWTMYYNG 180

Query: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240
           RKCGQA+SRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERV+G
Sbjct: 181 RKCGQAISRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVVG 240

Query: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 268
           SRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. NCBI nr
Match: XP_022966431.1 (protein MIZU-KUSSEI 1-like [Cucurbita maxima])

HSP 1 Score: 508.4 bits (1308), Expect = 3.6e-140
Identity = 254/267 (95.13%), Postives = 259/267 (97.00%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60
           MTGLQRTKSCSAAIKTTTKIIPSSIS HHLRSSSDADNFS KFLLQKNSSHRCHRQHLAA
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISGHHLRSSSDADNFSAKFLLQKNSSHRCHRQHLAA 60

Query: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120
           STTGKFS LLHSLLKL+SIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFA+QLD
Sbjct: 61  STTGKFSSLLHSLLKLISIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAVQLD 120

Query: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180
           PRS+PVLLLEFATSTSSLIKEMSSGLVRIALESDA TT+DGNR IRKLLEEPRWTMYCNG
Sbjct: 121 PRSEPVLLLEFATSTSSLIKEMSSGLVRIALESDALTTYDGNRSIRKLLEEPRWTMYCNG 180

Query: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240
           RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDG  GS SEGELLYMRAKFERV+G
Sbjct: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGHMGSRSEGELLYMRAKFERVVG 240

Query: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 268
           SRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. NCBI nr
Match: XP_038877466.1 (protein MIZU-KUSSEI 1-like [Benincasa hispida])

HSP 1 Score: 409.1 bits (1050), Expect = 3.0e-110
Identity = 217/282 (76.95%), Postives = 235/282 (83.33%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSIS-----AHHLRSSSDA---------DNFSGKFLLQ 60
           MTGLQRT+SC AAIKT+TK+IPSSIS      HHLRS S +         DNFSG+FLL 
Sbjct: 1   MTGLQRTRSCMAAIKTSTKLIPSSISDHQHRHHHLRSFSSSNLPPEYSNPDNFSGRFLLH 60

Query: 61  KNSSHRCHRQHLAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRR-VTGTL 120
           KNS H  H  H  A+   KFS LLHSLLKLLS+PTCKW SIPTHLSVS SLGRR VTGTL
Sbjct: 61  KNSGH--HGHHHQAAAGSKFSTLLHSLLKLLSVPTCKWFSIPTHLSVSSSLGRRVVTGTL 120

Query: 121 FGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPI 180
           FGRRRGHVTFA+QLDPRS+PVLLLE ATSTS L+KEMSSGLVRIALES+AP  ++ +RP 
Sbjct: 121 FGRRRGHVTFAVQLDPRSEPVLLLELATSTSFLVKEMSSGLVRIALESEAP-AYNESRP- 180

Query: 181 RKLLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSE 240
           RKL EEPRWTMYCNGRKCG +  R CGEFEWYVLNTVQ VSVGAGVIP+VDDGRKGS SE
Sbjct: 181 RKLFEEPRWTMYCNGRKCGHSTLRVCGEFEWYVLNTVQRVSVGAGVIPVVDDGRKGSRSE 240

Query: 241 GELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
           GELLYMRA +ERV+GSRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 GELLYMRANYERVVGSRDSEAFYMMNPDGNGGPELSIFLLRI 278

[Close]

BLAST of Carg21134 vs. ExPASy Swiss-Prot
Match: O22227 (Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1)

HSP 1 Score: 246.5 bits (628), Expect = 3.4e-64
Identity = 140/242 (57.85%), Postives = 169/242 (69.83%), Query Frame = 0


Query: 48  NSSHRCHRQHLAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSV-------------- 107
           ++SH   + HL      KFS LL S + +++IP CK LS+P+  S               
Sbjct: 67  SASHNIGKFHL------KFSLLLRSFINIINIPACKMLSLPSPPSSSSSVSNQLISLVTG 126

Query: 108 -SPSLGRRVTGTLFGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALE 167
            S SLGRRVTGTL+G +RGHVTF++Q + RS PVLLL+ A ST++L+KEMSSGLVRIALE
Sbjct: 127 GSSSLGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALE 186

Query: 168 SDAPTTFDGNRPIRKLLEEPRWTMYCNGRKCGQAMSR--TCGEFEWYVLNTVQSVSVGAG 227
            +       +R   KL +EP+WTMYCNGRKCG A+SR   C + +W VLNTV  V+VGAG
Sbjct: 187 CE-----KRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAG 246

Query: 228 VIP---MVDD-GRKGSGSE-GELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLL 268
           VIP    +DD    GSG+E GELLYMR KFERV+GSRDSEAFYMMNPD NGGPELSIFLL
Sbjct: 247 VIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLL 297

[Close]

BLAST of Carg21134 vs. ExPASy TrEMBL
Match: A0A6J1EAS0 (protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111432316 PE=4 SV=1)

HSP 1 Score: 528.9 bits (1361), Expect = 1.3e-146
Identity = 265/267 (99.25%), Postives = 266/267 (99.63%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60
           MTGLQRTKSCSAAI+TTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA
Sbjct: 1   MTGLQRTKSCSAAIQTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60

Query: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120
           STTGKFS LLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD
Sbjct: 61  STTGKFSSLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120

Query: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180
           PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG
Sbjct: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180

Query: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240
           RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG
Sbjct: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240

Query: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 268
           SRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. ExPASy TrEMBL
Match: A0A6J1HPB2 (protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466084 PE=4 SV=1)

HSP 1 Score: 508.4 bits (1308), Expect = 1.8e-140
Identity = 254/267 (95.13%), Postives = 259/267 (97.00%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISAHHLRSSSDADNFSGKFLLQKNSSHRCHRQHLAA 60
           MTGLQRTKSCSAAIKTTTKIIPSSIS HHLRSSSDADNFS KFLLQKNSSHRCHRQHLAA
Sbjct: 1   MTGLQRTKSCSAAIKTTTKIIPSSISGHHLRSSSDADNFSAKFLLQKNSSHRCHRQHLAA 60

Query: 61  STTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAIQLD 120
           STTGKFS LLHSLLKL+SIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFA+QLD
Sbjct: 61  STTGKFSSLLHSLLKLISIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVTFAVQLD 120

Query: 121 PRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTMYCNG 180
           PRS+PVLLLEFATSTSSLIKEMSSGLVRIALESDA TT+DGNR IRKLLEEPRWTMYCNG
Sbjct: 121 PRSEPVLLLEFATSTSSLIKEMSSGLVRIALESDALTTYDGNRSIRKLLEEPRWTMYCNG 180

Query: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFERVIG 240
           RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDG  GS SEGELLYMRAKFERV+G
Sbjct: 181 RKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGHMGSRSEGELLYMRAKFERVVG 240

Query: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 268
           SRDSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 SRDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. ExPASy TrEMBL
Match: A0A6J1DFH1 (protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111020015 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 5.3e-105
Identity = 205/276 (74.28%), Postives = 227/276 (82.25%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIP-SSISAH-----HLRSSSDADNFSGKFLLQKNSSHRCH 60
           M+ LQRT+SCSAAIKTTTKI P +S+S H     H+RSSS +D FSG  LLQK S    H
Sbjct: 1   MSTLQRTRSCSAAIKTTTKITPAASLSGHHHRHRHVRSSSSSDGFSGNLLLQKKSGD--H 60

Query: 61  RQHLAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRRVTGTLFGRRRGHVT 120
             H A    GKFS L HSLL+LLSIPTCKWLSIP HLS +PSL R+VTGTLFG RRGHVT
Sbjct: 61  HHHPA----GKFSSLFHSLLRLLSIPTCKWLSIPNHLSATPSLRRKVTGTLFGHRRGHVT 120

Query: 121 FAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRW 180
           FA+QL+PRS+PV LLE A ST+SL+KEMSSGLVRIALES+AP   DG    R+L EEPRW
Sbjct: 121 FAVQLEPRSEPVFLLELAVSTASLVKEMSSGLVRIALESEAPANEDGR---RRLFEEPRW 180

Query: 181 TMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGS---GSEGELLYM 240
           TMYCNGRKCG A SRTCG+FEW+VLNTVQSVSVGAGVIP+VDDGRKG    GS+GELLYM
Sbjct: 181 TMYCNGRKCGHATSRTCGDFEWHVLNTVQSVSVGAGVIPVVDDGRKGGGGCGSDGELLYM 240

Query: 241 RAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
           RA+FERV+GS DSEAFYMMNPDGNGGPELSIFLLRI
Sbjct: 241 RARFERVVGSHDSEAFYMMNPDGNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. ExPASy TrEMBL
Match: A0A5A7SPQ9 (Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1702G00210 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 7.5e-99
Identity = 201/271 (74.17%), Postives = 221/271 (81.55%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISA--HHLRSSSDADNFSGKFLLQKN-SSHRCHRQH 60
           MT LQRT+S SAAIK TTK+IPSSIS   HH   S  + N   ++    N S H  HR  
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60

Query: 61  LAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRR-VTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKLLS+PTCKWLSIPTHLSV PSLGRR VTGTLFGRRRGHVTFA
Sbjct: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120

Query: 121 IQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTM 180
           +Q+ P+S+PVLLLE ATSTSSL+KEMSSGLVRIALES+AP   DG RP +KL EEPRWTM
Sbjct: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDG-RP-KKLFEEPRWTM 180

Query: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G K S SEGELLYMRAKFE
Sbjct: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240

Query: 241 RVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
           RV+GSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. ExPASy TrEMBL
Match: A0A1S3CNY4 (protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103502949 PE=4 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 7.5e-99
Identity = 201/271 (74.17%), Postives = 221/271 (81.55%), Query Frame = 0


Query: 1   MTGLQRTKSCSAAIKTTTKIIPSSISA--HHLRSSSDADNFSGKFLLQKN-SSHRCHRQH 60
           MT LQRT+S SAAIK TTK+IPSSIS   HH   S  + N   ++    N S H  HR  
Sbjct: 1   MTILQRTRSSSAAIKATTKLIPSSISGHRHHDFRSFSSSNLPPEYSNPNNFSGHHGHRHQ 60

Query: 61  LAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSVSPSLGRR-VTGTLFGRRRGHVTFA 120
               T  KFS LLHSLLKLLS+PTCKWLSIPTHLSV PSLGRR VTGTLFGRRRGHVTFA
Sbjct: 61  TV--TGSKFSILLHSLLKLLSVPTCKWLSIPTHLSVRPSLGRRVVTGTLFGRRRGHVTFA 120

Query: 121 IQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTFDGNRPIRKLLEEPRWTM 180
           +Q+ P+S+PVLLLE ATSTSSL+KEMSSGLVRIALES+AP   DG RP +KL EEPRWTM
Sbjct: 121 VQIHPQSEPVLLLELATSTSSLVKEMSSGLVRIALESEAPAYNDG-RP-KKLFEEPRWTM 180

Query: 181 YCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMVDDGRKGSGSEGELLYMRAKFE 240
           YCNGRKCG +M RTCGEF+ YVLN VQSVSVGAGVIP+V++G K S SEGELLYMRAKFE
Sbjct: 181 YCNGRKCGHSMLRTCGEFDRYVLNMVQSVSVGAGVIPVVENGGKSSRSEGELLYMRAKFE 240

Query: 241 RVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
           RV+GSRDSEAFYM+NPD NGGPELSIFLLRI
Sbjct: 241 RVVGSRDSEAFYMINPDSNGGPELSIFLLRI 267

[Close]

BLAST of Carg21134 vs. TAIR 10
Match: AT2G41660.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 246.5 bits (628), Expect = 2.4e-65
Identity = 140/242 (57.85%), Postives = 169/242 (69.83%), Query Frame = 0


Query: 48  NSSHRCHRQHLAASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLSV-------------- 107
           ++SH   + HL      KFS LL S + +++IP CK LS+P+  S               
Sbjct: 67  SASHNIGKFHL------KFSLLLRSFINIINIPACKMLSLPSPPSSSSSVSNQLISLVTG 126

Query: 108 -SPSLGRRVTGTLFGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALE 167
            S SLGRRVTGTL+G +RGHVTF++Q + RS PVLLL+ A ST++L+KEMSSGLVRIALE
Sbjct: 127 GSSSLGRRVTGTLYGHKRGHVTFSVQYNQRSDPVLLLDLAMSTATLVKEMSSGLVRIALE 186

Query: 168 SDAPTTFDGNRPIRKLLEEPRWTMYCNGRKCGQAMSR--TCGEFEWYVLNTVQSVSVGAG 227
            +       +R   KL +EP+WTMYCNGRKCG A+SR   C + +W VLNTV  V+VGAG
Sbjct: 187 CE-----KRHRSGTKLFQEPKWTMYCNGRKCGYAVSRGGACTDTDWRVLNTVSRVTVGAG 246

Query: 228 VIP---MVDD-GRKGSGSE-GELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLL 268
           VIP    +DD    GSG+E GELLYMR KFERV+GSRDSEAFYMMNPD NGGPELSIFLL
Sbjct: 247 VIPTPKTIDDVSGVGSGTELGELLYMRGKFERVVGSRDSEAFYMMNPDKNGGPELSIFLL 297

[Close]

BLAST of Carg21134 vs. TAIR 10
Match: AT5G23100.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 202.2 bits (513), Expect = 5.2e-52
Identity = 113/236 (47.88%), Postives = 141/236 (59.75%), Query Frame = 0


Query: 59  AASTTGKFSFLLHSLLKLLSIPTCKWLSIPTHLS------VSPSLGRRVTGTLFGRRRGH 118
           ++S++   S ++    KL S    +  S+   LS       +  LG RV GTLFG RRGH
Sbjct: 42  SSSSSSSSSNVITHKKKLQSFAVSRLRSVIATLSRARPGNQNSGLGSRVVGTLFGSRRGH 101

Query: 119 VTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALE-------------------- 178
           V F+IQ DP S P  L+E AT  S L+KEM+SGLVRIALE                    
Sbjct: 102 VHFSIQKDPNSPPAFLIELATPISGLVKEMASGLVRIALECDKGKEEEEGEEKNGTLRHG 161

Query: 179 -SDAPTTFDGNRPIRKLLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGV 238
             D   T       R+L+EEP W  YCNG+KCG A  R CGE E  VL  ++ VS+GAGV
Sbjct: 162 GGDKTKTTTTAAVSRRLVEEPMWRTYCNGKKCGFATRRECGEKEKKVLKALEMVSMGAGV 221

Query: 239 IPMVDDGRKGSGSEGELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
           +P  ++   G G  G+++YMRAKFER++GSRDSEAFYMMNPD NG PELSI+LLRI
Sbjct: 222 LPETEEIGGGGGGGGDIMYMRAKFERIVGSRDSEAFYMMNPDSNGAPELSIYLLRI 277

[Close]

BLAST of Carg21134 vs. TAIR 10
Match: AT3G25640.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 196.8 bits (499), Expect = 2.2e-50
Identity = 103/178 (57.87%), Postives = 127/178 (71.35%), Query Frame = 0


Query: 97  LGRRVTGTLFGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAP 156
           LG RV GTLFG RRGHV FA+Q DP   P +L++  T TS L++EM+SGLVRIALE+ A 
Sbjct: 94  LGFRVVGTLFGNRRGHVYFAVQDDPTRLPAVLIQLPTPTSVLVREMASGLVRIALETAAY 153

Query: 157 TTFDGNRPIRKLLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMV- 216
            T D     +KLLEE  W  YCNG+KCG A  + CGE EW VL  V  +++GAGV+P   
Sbjct: 154 KT-DSK---KKLLEESTWRTYCNGKKCGYAARKECGEAEWKVLKAVGPITMGAGVLPATT 213

Query: 217 ----DDGRKGSGSE-GELLYMRAKFERVIGSRDSEAFYMMNPD-GNGGPELSIFLLRI 268
               ++G    GSE GEL+YMRA+FERV+GSRDSEAFYMMNPD  +GGPELS++ LR+
Sbjct: 214 TTVDEEGNGAVGSEKGELMYMRARFERVVGSRDSEAFYMMNPDVSSGGPELSVYFLRV 267

[Close]

BLAST of Carg21134 vs. TAIR 10
Match: AT5G06990.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 171.0 bits (432), Expect = 1.3e-42
Identity = 88/177 (49.72%), Postives = 115/177 (64.97%), Query Frame = 0


Query: 98  GRRVTGTLFGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPT 157
           G RVTGTLFG R+  V  A+Q +PRS P+LLLE A  T  L++++  GLVRIALE +   
Sbjct: 90  GTRVTGTLFGYRKTRVNLAVQENPRSLPILLLELAIPTGKLLQDLGVGLVRIALECEKKP 149

Query: 158 TFDGNRPIRKLLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPM--- 217
           +        K+++EP W +YCNG+K G  + R   E +  V+  + +VS+GAGV+P+   
Sbjct: 150 S-----EKTKIIDEPIWALYCNGKKSGYGVKRQPTEEDLVVMQMLHAVSMGAGVLPVSSG 209

Query: 218 ----VDDGRKGSGSEGELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
                  G  G   EG+L YMRA FERVIGSRDSE +YMMNPDGN GPELSIF +R+
Sbjct: 210 AITEQSGGGGGGQQEGDLTYMRAHFERVIGSRDSETYYMMNPDGNSGPELSIFFVRV 261

[Close]

BLAST of Carg21134 vs. TAIR 10
Match: AT4G39610.1 (Protein of unknown function, DUF617 )

HSP 1 Score: 167.5 bits (423), Expect = 1.4e-41
Identity = 83/172 (48.26%), Postives = 116/172 (67.44%), Query Frame = 0


Query: 100 RVTGTLFGRRRGHVTFAIQLDPRSQPVLLLEFATSTSSLIKEMSSGLVRIALESDAPTTF 159
           R+TGTLFG R+G V+ +IQ +P+  P L++E A  T++L KE+S+G+VRIALE++     
Sbjct: 96  RITGTLFGYRKGRVSLSIQENPKCLPSLVVELAMQTTTLQKELSTGMVRIALETEKQPRA 155

Query: 160 DGNRPIRK----LLEEPRWTMYCNGRKCGQAMSRTCGEFEWYVLNTVQSVSVGAGVIPMV 219
           D N    +    +LEEP WTMYC G K G  + R   E +  V+  ++ VS+GAGV+P  
Sbjct: 156 DNNNSKTEKKTDILEEPLWTMYCKGEKTGYGVKREATEEDLNVMELLRPVSMGAGVLP-- 215

Query: 220 DDGRKGSGSEGELLYMRAKFERVIGSRDSEAFYMMNPDGNGGPELSIFLLRI 268
               +  G +GE+ YMRA FERVIGS+DSE FYM++P+GN GPELS F +R+
Sbjct: 216 -GNSESEGPDGEMAYMRAYFERVIGSKDSETFYMLSPEGNNGPELSFFFVRV 264

The following BLAST results are available for this feature:

BLAST of Carg21134 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027634.1	1.1e-147	100.00	Protein MIZU-KUSSEI 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022924911.1	2.6e-146	99.25	protein MIZU-KUSSEI 1 [Cucurbita moschata]	[more]
XP_023518638.1	2.7e-143	97.00	protein MIZU-KUSSEI 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022966431.1	3.6e-140	95.13	protein MIZU-KUSSEI 1-like [Cucurbita maxima]	[more]
XP_038877466.1	3.0e-110	76.95	protein MIZU-KUSSEI 1-like [Benincasa hispida]	[more]

BLAST of Carg21134 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O22227	3.4e-64	57.85	Protein MIZU-KUSSEI 1 OS=Arabidopsis thaliana OX=3702 GN=MIZ1 PE=1 SV=1	[more]

BLAST of Carg21134 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EAS0	1.3e-146	99.25	protein MIZU-KUSSEI 1 OS=Cucurbita moschata OX=3662 GN=LOC111432316 PE=4 SV=1	[more]
A0A6J1HPB2	1.8e-140	95.13	protein MIZU-KUSSEI 1-like OS=Cucurbita maxima OX=3661 GN=LOC111466084 PE=4 SV=1	[more]
A0A6J1DFH1	5.3e-105	74.28	protein MIZU-KUSSEI 1 OS=Momordica charantia OX=3673 GN=LOC111020015 PE=4 SV=1	[more]
A0A5A7SPQ9	7.5e-99	74.17	Protein MIZU-KUSSEI 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]
A0A1S3CNY4	7.5e-99	74.17	protein MIZU-KUSSEI 1-like OS=Cucumis melo OX=3656 GN=LOC103502949 PE=4 SV=1	[more]

BLAST of Carg21134 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G41660.1	2.4e-65	57.85	Protein of unknown function, DUF617	[more]
AT5G23100.1	5.2e-52	47.88	Protein of unknown function, DUF617	[more]
AT3G25640.1	2.2e-50	57.87	Protein of unknown function, DUF617	[more]
AT5G06990.1	1.3e-42	49.72	Protein of unknown function, DUF617	[more]
AT4G39610.1	1.4e-41	48.26	Protein of unknown function, DUF617	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PFAM	PF04759	DUF617	coord: 101..266 e-value: 9.6E-66 score: 220.9
IPR006460	Protein MIZU-KUSSEI 1-like, plant	TIGRFAM	TIGR01570	TIGR01570	coord: 101..267 e-value: 2.8E-66 score: 220.4
IPR006460	Protein MIZU-KUSSEI 1-like, plant	PANTHER	PTHR31696	PROTEIN MIZU-KUSSEI 1	coord: 35..267
None	No IPR available	PANTHER	PTHR31696:SF71	PROTEIN MIZU-KUSSEI 1	coord: 35..267

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21134-RA	Carg21134-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010274	hydrotropism
cellular_component	GO:0098827	endoplasmic reticulum subcompartment
cellular_component	GO:0016020	membrane