Carg21129 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21129
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Transcription initiation factor IIB
Location	Carg_Chr07: 7992616 .. 7996002 (+)
RNA-Seq Expression	Carg21129
Synteny	Carg21129

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AATTCCTCTCTCTATAAATACCCTCCTCTCCCACACTCACCATTCGCTTTTCCTCTTCCATCGTCAGAATCAAATTCGACGGCCGGCGGATTAACGAATCAATATCCTAAATTCTGCCGGAGTTAGGATTCGTTCTCTCTTCTGCTCCCTGAATTCTGATTTCTGAAGCTTTTTCTTTGGGATAGAGGAGGAGGAGGAAGAGGAGGGGGAGGAGGAGGAGGAGGAGGTTGTGAACAATGTCCGATGCGTTCTGCTCCGACTGCAAGCGTCAGACGGAGGTTGTCTTCGATCACTCTGCCGGAGACACCGTGTGCTCTGAGTGCGGCCTTGTGCTCGAGTCGCACTCCATCGATGAGACGTCCGAGTGGAGGACTTTTGCCAATGAGTCTGGGGATAACGACCCGGTTCGTGTTGGTGGACCGTCCAACCCGCTTTTGGCTGATGGAGGCCTTTCCACGGTGATTGCCAAGCCTAATGGTACGGCAGGGGAGTTCTTGTCCTCGTCATTAGGTCGGTGGCAGAATCGGGGGTCTAATCCAGATCGAGGGTTGATTCTTGCATTTAAGACAATTGCTACTATGTCTGATAGGTAAGGGGGTTGGTTGTTTTGATCGTCCTTTTGATGCTGAGGAAACATGGAATTAGTTGAATTTCTTTGGATTTAACTAAATCGTAATGTTAAACCGATCGAATTGGAATTGTGGAAGGTGGGAATTGGAAAGTCTTAGTAGTTTGATGATTTTTGTGTTCGTGGGGGAACTTTTGATGATGGATACTAAATTAGGGTATTTCGACTTTTAAGTCCGGATTCAATTAGGTCAATTTTTCTTGTTGCTTTGGAATTTGAAGGGTTTTCTTCATTATGGGGGCAGCCTCTTCTCTCTAATGGGATTTTAAAACTGATAAACTTAATGTTAATCATTCCATCTAGTGTTTGATAGTTTTATAATTGGCCTTGCCTCATGATCATGAATCTTTCTATGATTTTGTTTTCTTATTTTCATGCATTTTGTGATTGAGCTGCTTATTTACAAGTGTCCTAGCTTTGATGATAACAATCTGAGACTGATATATAATGCTTAATGAAGTCAATGTTGAACCCAAAGCTCAGATACTGTTTGTTGTGTTGTAATGTCACGACTAAAAATATTCTGATATCCAATTGTTGTACCGGAAGCCATAACCAAAATGTTTTGATACCCAATTATTGTAGTGGTATGCCACGACAAAAAAAAAAAAAAAAAAAAAAAGCTTTGATACCCAATTGTTGTAGGGTAATACCGAATACCCAATTGTTGTACCAAAAGTCCCCTAACCAAAGTGTTTACAAAGTGTTTCTCGTACTCTCACGCTACAAGACAATAGTTTTTGAGCCAGAGTGCTATTTGTTTGGTTGTGGCTTCCGCTACACCAATTGGGTATCAAAGCATTTTTTGGTTGTGGCATTCCGCTTCAACAATTGGATATTAGAACATTTTTTGTTGTGGCATTACGTGGGATCAGAGCTTTGGGTACAACAGTCATGAGTTTGCATATTTTGTTAAATTGTGCTTTTTTGTTTTAGTTCAGCTCCTCTTCATGGGCCATTTTCTTTTGATGCCCTTTGTTTTCTTTCATCTTTCTCAACAAAACTTGGCATTTAATCAACAAAAGAAAGAAAAAGAAAAGGTCACACCTGACTAATGTTAGCTGTAATGTGTTTCAGGTTGGGCCTTGTTGCAACCATTAAGGTATGCAATTTTCCATCCATTATGACAAAATGACACGAGTTACCATTTCTTGTATTTTCTCAAAGCATTCAGGTTAGTTGTCAGTATAGAATGATCATGCTGTATTGTCAAGGTTGTAAATAATGTATTTTTCACCGTCTGCATTATACTTTGGTTCTGGAATTGATGCACGATGAATAATTCGAAGAACAAAATAGTTTTAGATTCGAAGACCACAATAGTTTTAGATTTTGTTTTTGAGTTCCATTCATTTCGCTAGCAGTGAGCTTTGGTATTTTGTAAATGTTTTATTCATAGGATCGGGCGAATGAGATATATAAAAGAGTTGAAGATCAAAAGTCTAGTAGAGGAAGAAATCAAGATGCTTTATTGGCTGCTTGCTTATACATTGCTTGTCGACAAGAAGACAAGCCCCGCACAGTCAAGGGTACTGCTTGTAAATCATTTGATATCTGGAGCATTTGTAGTTCGATTTCTCTTCTTTACCTAATCATGCTGGTTTTGAGTAGAAATTTGCTCTGTTGCTAATGGGGCAACAAAGAAGGAGATTGGCCGAGCAAAAGAATACATCGTGAAACAGTTGGGGTTGGAGACGGGTCAGTCTGTGGAGATGGGAACGATACATGCTGGAGACTTTATGGTGAGAATATCAACTTAATTATTCTAAAGTTCGATCATGGATAATGTTGTGTTCATTTCTTCTGACATCAAGTTTCTTTCCTAAATGAACATAGAGGCGTTTTTGTTCTAATCTTGGCATGAATAATCAAGCTGTTAAAGCTGCCCAAGAAGCTGTACAGAAATCTGAAGAGTTCGACATTAGGTAACGATGTAATGATTTGGATATTATGAATTTCTTTCTTTGCTTCTTTTATGGTTATTTATTTGGGTGGTTTGATAAGTGTTCACTCAACTTCGATGAAATCCGCCCCGTACACATACCTCTGCTGGTCTGGCATAGTAGCCAACTTCTACGAAATCTGCAGTGCCTAAGTAGTATTGATATCCACTGGCCTAGCTAAGTAACTGCTTGGTTGAATCCCTTGTTCTTTAACATATAATTTGATTGATTGATTTATCCTCAGCTTATCAAACTGTTTGAATTCATGCTTTGATTAGAAAACATTGGATAAAGTTTTGAATAAGCTTGGTGTTAACCTTAGTAGGCACCATTTTCTAGGGACAGGATTATTGTTGGTTCACTTTTTTCTTAAAGAGGATTGTCTTCCCTCATTTCAGGAGAAGCCCAATATCCATTGCTGCAGCAGTTATTTACATTATTACTCAGCTTTCAGATGATAAGAAGCCTCTAAAAGGTAATGTTTAACTGTGGCTATAATCTTCACTACATCATATATGTTCATAATCTGTTCATTTGGGCATGTTTTGTTTGATAATGACTCTGCTATTTGATTACGAAAGTTATGGTTTTCTCCTCGAAACAAAAGTAGACCCGTTCTCCCAAGAGCATTGTTGTCGTCAGTCTTGATATGAAATTTAATCAAGGGATTTGAGTTTTACAGACATATCGGTAGCTACCGGTGTTGCGGAAGGGACGATCAGAAACTCATATAAAGATCTGTATCCGCATGTTTCGAAGATAATACCGGGGTGGTATGCTAAAGAGGAGGATCTTAAGAACCTGTGCAGTCCTTGA

mRNA sequence

AATTCCTCTCTCTATAAATACCCTCCTCTCCCACACTCACCATTCGCTTTTCCTCTTCCATCGTCAGAATCAAATTCGACGGCCGGCGGATTAACGAATCAATATCCTAAATTCTGCCGGAGTTAGGATTCGTTCTCTCTTCTGCTCCCTGAATTCTGATTTCTGAAGCTTTTTCTTTGGGATAGAGGAGGAGGAGGAAGAGGAGGGGGAGGAGGAGGAGGAGGAGGTTGTGAACAATGTCCGATGCGTTCTGCTCCGACTGCAAGCGTCAGACGGAGGTTGTCTTCGATCACTCTGCCGGAGACACCGTGTGCTCTGAGTGCGGCCTTGTGCTCGAGTCGCACTCCATCGATGAGACGTCCGAGTGGAGGACTTTTGCCAATGAGTCTGGGGATAACGACCCGGTTCGTGTTGGTGGACCGTCCAACCCGCTTTTGGCTGATGGAGGCCTTTCCACGGTGATTGCCAAGCCTAATGGTACGGCAGGGGAGTTCTTGTCCTCGTCATTAGGTCGGTGGCAGAATCGGGGGTCTAATCCAGATCGAGGGTTGATTCTTGCATTTAAGACAATTGCTACTATGATCGGGCGAATGAGATATATAAAAGAGTTGAAGATCAAAAGTCTAGTAGAGGAAGAAATCAAGATGCTTTATTGGCTGCTTGCTTATACATTGCTTGTCGACAAGAAGACAAGCCCCGCACAGTCAAGGAAGGAGATTGGCCGAGCAAAAGAATACATCGTGAAACAGTTGGGGTTGGAGACGGGTCAGTCTGTGGAGATGGGAACGATACATGCTGGAGACTTTATGAGGCGTTTTTGTTCTAATCTTGGCATGAATAATCAAGCTGTTAAAGCTGCCCAAGAAGCTGTACAGAAATCTGAAGAGTTCGACATTAGGAGAAGCCCAATATCCATTGCTGCAGCAGTTATTTACATTATTACTCAGCTTTCAGATGATAAGAAGCCTCTAAAAGACATATCGGTAGCTACCGGTGTTGCGGAAGGGACGATCAGAAACTCATATAAAGATCTGTATCCGCATGTTTCGAAGATAATACCGGGGTGGTATGCTAAAGAGGAGGATCTTAAGAACCTGTGCAGTCCTTGA

Coding sequence (CDS)

ATGTCCGATGCGTTCTGCTCCGACTGCAAGCGTCAGACGGAGGTTGTCTTCGATCACTCTGCCGGAGACACCGTGTGCTCTGAGTGCGGCCTTGTGCTCGAGTCGCACTCCATCGATGAGACGTCCGAGTGGAGGACTTTTGCCAATGAGTCTGGGGATAACGACCCGGTTCGTGTTGGTGGACCGTCCAACCCGCTTTTGGCTGATGGAGGCCTTTCCACGGTGATTGCCAAGCCTAATGGTACGGCAGGGGAGTTCTTGTCCTCGTCATTAGGTCGGTGGCAGAATCGGGGGTCTAATCCAGATCGAGGGTTGATTCTTGCATTTAAGACAATTGCTACTATGATCGGGCGAATGAGATATATAAAAGAGTTGAAGATCAAAAGTCTAGTAGAGGAAGAAATCAAGATGCTTTATTGGCTGCTTGCTTATACATTGCTTGTCGACAAGAAGACAAGCCCCGCACAGTCAAGGAAGGAGATTGGCCGAGCAAAAGAATACATCGTGAAACAGTTGGGGTTGGAGACGGGTCAGTCTGTGGAGATGGGAACGATACATGCTGGAGACTTTATGAGGCGTTTTTGTTCTAATCTTGGCATGAATAATCAAGCTGTTAAAGCTGCCCAAGAAGCTGTACAGAAATCTGAAGAGTTCGACATTAGGAGAAGCCCAATATCCATTGCTGCAGCAGTTATTTACATTATTACTCAGCTTTCAGATGATAAGAAGCCTCTAAAAGACATATCGGTAGCTACCGGTGTTGCGGAAGGGACGATCAGAAACTCATATAAAGATCTGTATCCGCATGTTTCGAAGATAATACCGGGGTGGTATGCTAAAGAGGAGGATCTTAAGAACCTGTGCAGTCCTTGA

Protein sequence

MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMRYIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWYAKEEDLKNLCSP

Homology

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BLAST of Carg21129 vs. NCBI nr
Match: KAG7027639.1 (Transcription initiation factor IIB-2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 580.1 bits (1494), Expect = 1.1e-161
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR
Sbjct: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120

Query: 121 YIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGRAKEYIVKQLGLETGQSV 180
           YIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGRAKEYIVKQLGLETGQSV
Sbjct: 121 YIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGRAKEYIVKQLGLETGQSV 180

Query: 181 EMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQLSD 240
           EMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQLSD
Sbjct: 181 EMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQLSD 240

Query: 241 DKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWYAKEEDLKNLCSP 291
           DKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWYAKEEDLKNLCSP
Sbjct: 241 DKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWYAKEEDLKNLCSP 290

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BLAST of Carg21129 vs. NCBI nr
Match: GAY66967.1 (hypothetical protein CUMW_253010 [Citrus unshiu])

HSP 1 Score: 537.7 bits (1384), Expect = 6.1e-149
Identity = 272/293 (92.83%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           M+DAFCSDCK+ TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MTDAFCSDCKKHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATM---IG 120
           GP+NPLLADGGLSTVIAKPNG +GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM   IG
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRIG 120

Query: 121 RMRYIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGRAKEYIVKQLGLETG 180
           +MRYI+  KIKSLVE EIK  YWLLA TLLVDKKTS A  RKEIGRAKEYIVKQLGLETG
Sbjct: 121 QMRYIRRWKIKSLVEAEIKTHYWLLACTLLVDKKTSHALLRKEIGRAKEYIVKQLGLETG 180

Query: 181 QSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQ 240
           QSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQ
Sbjct: 181 QSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQ 240

Query: 241 LSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWYAKEEDLKNLCSP 291
           LSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIP WYAKEEDLKNLCSP
Sbjct: 241 LSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPNWYAKEEDLKNLCSP 293

[Close]

BLAST of Carg21129 vs. NCBI nr
Match: XP_022924909.1 (transcription initiation factor IIB-2-like [Cucurbita moschata] >XP_023518635.1 transcription initiation factor IIB-2-like [Cucurbita pepo subsp. pepo] >KAG6595678.1 Transcription initiation factor IIB-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 487.3 bits (1253), Expect = 9.4e-134
Identity = 258/312 (82.69%), Postives = 268/312 (85.90%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 300

[Close]

BLAST of Carg21129 vs. NCBI nr
Match: XP_022152227.1 (transcription initiation factor IIB-2 [Momordica charantia] >XP_022928717.1 transcription initiation factor IIB-2 [Cucurbita moschata] >XP_022967855.1 transcription initiation factor IIB-2 [Cucurbita maxima] >XP_023544538.1 transcription initiation factor IIB-2 [Cucurbita pepo subsp. pepo] >KAG6603201.1 Transcription initiation factor IIB-2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 485.0 bits (1247), Expect = 4.7e-133
Identity = 256/312 (82.05%), Postives = 268/312 (85.90%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGLSTVIAKPNGT+GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGTSGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 300

[Close]

BLAST of Carg21129 vs. NCBI nr
Match: XP_004150610.1 (transcription initiation factor IIB-2 [Cucumis sativus] >XP_008455781.1 PREDICTED: transcription initiation factor IIB-2 [Cucumis melo] >KAA0043603.1 transcription initiation factor IIB-2-like [Cucumis melo var. makuwa] >KGN54643.1 hypothetical protein Csa_012330 [Cucumis sativus] >TYK29758.1 transcription initiation factor IIB-2-like [Cucumis melo var. makuwa])

HSP 1 Score: 483.8 bits (1244), Expect = 1.0e-132
Identity = 255/312 (81.73%), Postives = 267/312 (85.58%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGLSTVIAKPNGT GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGTTGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKI+PGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKILPGWY 300

[Close]

BLAST of Carg21129 vs. ExPASy Swiss-Prot
Match: Q9SS44 (Transcription initiation factor IIB-2 OS=Arabidopsis thaliana OX=3702 GN=TFIIB2 PE=2 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 3.6e-128
Identity = 241/312 (77.24%), Postives = 262/312 (83.97%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKR TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGL+TVI+KPNG++G+FLSSSLGRWQNRGSNPDRGLI+AFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLTTVISKPNGSSGDFLSSSLGRWQNRGSNPDRGLIVAFKTIATMADRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQ VEMGTIHAGDFMRRFCSNLGM NQ VKAAQE+VQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQLVEMGTIHAGDFMRRFCSNLGMTNQTVKAAQESVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSD+KKPL+DISVATGVAEGTIRNSYKDLYPH+SKIIP WY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDEKKPLRDISVATGVAEGTIRNSYKDLYPHLSKIIPAWY 300

[Close]

BLAST of Carg21129 vs. ExPASy Swiss-Prot
Match: P48513 (Transcription initiation factor IIB OS=Glycine max OX=3847 GN=TFIIB1 PE=2 SV=1)

HSP 1 Score: 457.6 bits (1176), Expect = 1.0e-127
Identity = 246/313 (78.59%), Postives = 258/313 (82.43%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDP RVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPNRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPN-GTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRM 120
           GPSNPLL DGGLSTVIAKPN G  GEFLSSSLGRWQNRGSNPDR LI AFKTIATM  R+
Sbjct: 61  GPSNPLLTDGGLSTVIAKPNGGGGGEFLSSSLGRWQNRGSNPDRALIQAFKTIATMSDRL 120

Query: 121 RYIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQS 180
             +  +K     I   VE++     +    LLA  L +     DK          +   +
Sbjct: 121 GLVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGAT 180

Query: 181 RKEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEE 240
           +KEIGRAKEYIVKQLGLE G +VEMGTIHAGDFMRRFCSNL MNNQAVKAAQEAVQKSEE
Sbjct: 181 KKEIGRAKEYIVKQLGLENGNAVEMGTIHAGDFMRRFCSNLCMNNQAVKAAQEAVQKSEE 240

Query: 241 FDIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGW 291
           FDIRRSPISIAAAVIYIITQLSDDKKPLKDIS+ATGVAEGTIRNSYKDLYPHVSKIIP W
Sbjct: 241 FDIRRSPISIAAAVIYIITQLSDDKKPLKDISLATGVAEGTIRNSYKDLYPHVSKIIPNW 300

[Close]

BLAST of Carg21129 vs. ExPASy Swiss-Prot
Match: Q8W0W3 (Transcription initiation factor IIB OS=Oryza sativa subsp. japonica OX=39947 GN=TFIIB PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.1e-119
Identity = 221/312 (70.83%), Postives = 253/312 (81.09%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSD+FC DCK+ TEV FDHSAGDTVC+ECGLVLE+HS+DETSEWRTFANES DNDPVRVG
Sbjct: 1   MSDSFCPDCKKHTEVAFDHSAGDTVCTECGLVLEAHSVDETSEWRTFANESSDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLL DGGLSTVIAKPNG  GEFLSSSLGRWQNRGSNPDR LILAF+TIA M  R+ 
Sbjct: 61  GPTNPLLTDGGLSTVIAKPNGAQGEFLSSSLGRWQNRGSNPDRSLILAFRTIANMADRLG 120

Query: 121 YIKELKIKS----LVEEEIKMLYW-----LLAYTLLV-----DK--------KTSPAQSR 180
            +  +K ++       E++K +       +LA  L +     D+          +   ++
Sbjct: 121 LVATIKDRANEIYKKVEDLKSIRGRNQDAILAACLYIACRQEDRPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKE+IVKQL +E GQS+EMGTIHAGDF+RRFCS LGMNNQAVKAAQEAVQ+SEE 
Sbjct: 181 KEIGRAKEFIVKQLEVEMGQSMEMGTIHAGDFLRRFCSTLGMNNQAVKAAQEAVQRSEEL 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIY+ITQLSDDKKPLKDIS+ATGVAEGTIRNSYKDLYP+ S++IP  Y
Sbjct: 241 DIRRSPISIAAAVIYMITQLSDDKKPLKDISLATGVAEGTIRNSYKDLYPYASRLIPNTY 300

[Close]

BLAST of Carg21129 vs. ExPASy Swiss-Prot
Match: P48512 (Transcription initiation factor IIB-1 OS=Arabidopsis thaliana OX=3702 GN=TFIIB1 PE=1 SV=1)

HSP 1 Score: 417.5 bits (1072), Expect = 1.2e-115
Identity = 219/312 (70.19%), Postives = 247/312 (79.17%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDA+C+DCK++TE+V DHSAGDT+CSECGLVLESHSIDETSEWRTFANES ++DP RVG
Sbjct: 1   MSDAYCTDCKKETELVVDHSAGDTLCSECGLVLESHSIDETSEWRTFANESSNSDPNRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLAD  L+TVIAKPNG++G+FLSSSLGRWQNR SN DRGLI AFKTIATM  R+ 
Sbjct: 61  GPTNPLLADSALTTVIAKPNGSSGDFLSSSLGRWQNRNSNSDRGLIQAFKTIATMSERLG 120

Query: 121 YIKELK------IKSLVEEEIK------MLYWLLAY----------TLLVDKKTSPAQSR 180
            +  +K       K L +++         LY    Y          T+      +   ++
Sbjct: 121 LVATIKDRANELYKRLEDQKSSRGRNQDALYAACLYIACRQEDKPRTIKEICVIANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAK+YIVK LGLE GQSV++GTIHAGDFMRRFCSNL M+N AVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKDYIVKTLGLEPGQSVDLGTIHAGDFMRRFCSNLAMSNHAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAA VIYIITQLSDDKK LKDIS ATGVAEGTIRNSYKDLYPH+SKI P WY
Sbjct: 241 DIRRSPISIAAVVIYIITQLSDDKKTLKDISHATGVAEGTIRNSYKDLYPHLSKIAPSWY 300

[Close]

BLAST of Carg21129 vs. ExPASy Swiss-Prot
Match: Q9LIA6 (Plant-specific TFIIB-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=PBRP2 PE=2 SV=1)

HSP 1 Score: 198.7 bits (504), Expect = 8.8e-50
Identity = 121/319 (37.93%), Postives = 176/319 (55.17%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANES--GDNDPVR 60
           M +  C DCKR T +V DHS+GDT+CSECGLVLE+H I+ + EWRTFA++    D DP R
Sbjct: 1   MEEETCLDCKRPTIMVVDHSSGDTICSECGLVLEAHIIEYSQEWRTFASDDNHSDRDPNR 60

Query: 61  VGGPSNPLLADGGLSTVIAKPNGTAGEFLS----SSLGRWQNRGSNPDRGLI-LAFKTIA 120
           VG  +NP L  G L T+I KP  TA   LS    S+L R  N+  N +  LI  AF+ I 
Sbjct: 61  VGAATNPFLKSGDLVTIIEKPKETASSVLSKDDISTLFRAHNQVKNHEEDLIKQAFEEIQ 120

Query: 121 TMIG-------------------------RMRYIKELKIKSLVEEEIKMLYWLLAYTLLV 180
            M                           ++R  K+L                L+ TL  
Sbjct: 121 RMTDALDLDIVINSRACEIVSKYDGHANTKLRRGKKLNAICAASVSTACRELQLSRTLKE 180

Query: 181 DKKTSPAQSRKEIGRAKEYIVKQL--GLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVK 240
             + +    +K+I R +  ++K++    +T  S     I+ G+ +RRFCS L ++ + + 
Sbjct: 181 IAEVANGVDKKDI-RKESLVIKRVLESHQTSVSASQAIINTGELVRRFCSKLDISQREIM 240

Query: 241 AAQEAVQKSEEFDIRRSPISIAAAVIYIITQLSD-DKKPLKDISVATGVAEGTIRNSYKD 285
           A  EAV+K+E FDIRR+P S+ AA+I++I+ +S  ++KP+++I +   V E TI+NS KD
Sbjct: 241 AIPEAVEKAENFDIRRNPKSVLAAIIFMISHISQTNRKPIREIGIVAEVVENTIKNSVKD 300

[Close]

BLAST of Carg21129 vs. ExPASy TrEMBL
Match: A0A2H5QQS2 (TFIIB-type domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_253010 PE=4 SV=1)

HSP 1 Score: 537.7 bits (1384), Expect = 2.9e-149
Identity = 272/293 (92.83%), Postives = 278/293 (94.88%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           M+DAFCSDCK+ TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MTDAFCSDCKKHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATM---IG 120
           GP+NPLLADGGLSTVIAKPNG +GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM   IG
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGASGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRIG 120

Query: 121 RMRYIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGRAKEYIVKQLGLETG 180
           +MRYI+  KIKSLVE EIK  YWLLA TLLVDKKTS A  RKEIGRAKEYIVKQLGLETG
Sbjct: 121 QMRYIRRWKIKSLVEAEIKTHYWLLACTLLVDKKTSHALLRKEIGRAKEYIVKQLGLETG 180

Query: 181 QSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQ 240
           QSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQ
Sbjct: 181 QSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEFDIRRSPISIAAAVIYIITQ 240

Query: 241 LSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWYAKEEDLKNLCSP 291
           LSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIP WYAKEEDLKNLCSP
Sbjct: 241 LSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPNWYAKEEDLKNLCSP 293

[Close]

BLAST of Carg21129 vs. ExPASy TrEMBL
Match: A0A6J1EAJ6 (transcription initiation factor IIB-2-like OS=Cucurbita moschata OX=3662 GN=LOC111432315 PE=3 SV=1)

HSP 1 Score: 487.3 bits (1253), Expect = 4.6e-134
Identity = 258/312 (82.69%), Postives = 268/312 (85.90%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 300

[Close]

BLAST of Carg21129 vs. ExPASy TrEMBL
Match: A0A6J1HVL0 (transcription initiation factor IIB-2 OS=Cucurbita maxima OX=3661 GN=LOC111467238 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.3e-133
Identity = 256/312 (82.05%), Postives = 268/312 (85.90%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGLSTVIAKPNGT+GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGTSGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 300

[Close]

BLAST of Carg21129 vs. ExPASy TrEMBL
Match: A0A6J1DFL4 (transcription initiation factor IIB-2 OS=Momordica charantia OX=3673 GN=LOC111019998 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.3e-133
Identity = 256/312 (82.05%), Postives = 268/312 (85.90%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGLSTVIAKPNGT+GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGTSGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 300

[Close]

BLAST of Carg21129 vs. ExPASy TrEMBL
Match: A0A6J1EKQ3 (transcription initiation factor IIB-2 OS=Cucurbita moschata OX=3662 GN=LOC111435544 PE=3 SV=1)

HSP 1 Score: 485.0 bits (1247), Expect = 2.3e-133
Identity = 256/312 (82.05%), Postives = 268/312 (85.90%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGLSTVIAKPNGT+GEFLSSSLGRWQNRGSNPDRGLILAFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLSTVIAKPNGTSGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMSDRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 300

[Close]

BLAST of Carg21129 vs. TAIR 10
Match: AT3G10330.1 (Cyclin-like family protein )

HSP 1 Score: 459.1 bits (1180), Expect = 2.6e-129
Identity = 241/312 (77.24%), Postives = 262/312 (83.97%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDAFCSDCKR TEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG
Sbjct: 1   MSDAFCSDCKRHTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLADGGL+TVI+KPNG++G+FLSSSLGRWQNRGSNPDRGLI+AFKTIATM  R+ 
Sbjct: 61  GPTNPLLADGGLTTVISKPNGSSGDFLSSSLGRWQNRGSNPDRGLIVAFKTIATMADRLG 120

Query: 121 YIKELK-----IKSLVEEEI----KMLYWLLAYTLLV-----DK--------KTSPAQSR 180
            +  +K     I   VE++     +    LLA  L +     DK          +   ++
Sbjct: 121 LVATIKDRANEIYKRVEDQKSSRGRNQDALLAACLYIACRQEDKPRTVKEICSVANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAKEYIVKQLGLETGQ VEMGTIHAGDFMRRFCSNLGM NQ VKAAQE+VQKSEEF
Sbjct: 181 KEIGRAKEYIVKQLGLETGQLVEMGTIHAGDFMRRFCSNLGMTNQTVKAAQESVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAAAVIYIITQLSD+KKPL+DISVATGVAEGTIRNSYKDLYPH+SKIIP WY
Sbjct: 241 DIRRSPISIAAAVIYIITQLSDEKKPLRDISVATGVAEGTIRNSYKDLYPHLSKIIPAWY 300

[Close]

BLAST of Carg21129 vs. TAIR 10
Match: AT2G41630.1 (transcription factor IIB )

HSP 1 Score: 417.5 bits (1072), Expect = 8.5e-117
Identity = 219/312 (70.19%), Postives = 247/312 (79.17%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVG 60
           MSDA+C+DCK++TE+V DHSAGDT+CSECGLVLESHSIDETSEWRTFANES ++DP RVG
Sbjct: 1   MSDAYCTDCKKETELVVDHSAGDTLCSECGLVLESHSIDETSEWRTFANESSNSDPNRVG 60

Query: 61  GPSNPLLADGGLSTVIAKPNGTAGEFLSSSLGRWQNRGSNPDRGLILAFKTIATMIGRMR 120
           GP+NPLLAD  L+TVIAKPNG++G+FLSSSLGRWQNR SN DRGLI AFKTIATM  R+ 
Sbjct: 61  GPTNPLLADSALTTVIAKPNGSSGDFLSSSLGRWQNRNSNSDRGLIQAFKTIATMSERLG 120

Query: 121 YIKELK------IKSLVEEEIK------MLYWLLAY----------TLLVDKKTSPAQSR 180
            +  +K       K L +++         LY    Y          T+      +   ++
Sbjct: 121 LVATIKDRANELYKRLEDQKSSRGRNQDALYAACLYIACRQEDKPRTIKEICVIANGATK 180

Query: 181 KEIGRAKEYIVKQLGLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF 240
           KEIGRAK+YIVK LGLE GQSV++GTIHAGDFMRRFCSNL M+N AVKAAQEAVQKSEEF
Sbjct: 181 KEIGRAKDYIVKTLGLEPGQSVDLGTIHAGDFMRRFCSNLAMSNHAVKAAQEAVQKSEEF 240

Query: 241 DIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKIIPGWY 291
           DIRRSPISIAA VIYIITQLSDDKK LKDIS ATGVAEGTIRNSYKDLYPH+SKI P WY
Sbjct: 241 DIRRSPISIAAVVIYIITQLSDDKKTLKDISHATGVAEGTIRNSYKDLYPHLSKIAPSWY 300

[Close]

BLAST of Carg21129 vs. TAIR 10
Match: AT3G29380.1 (Cyclin-like family protein )

HSP 1 Score: 198.7 bits (504), Expect = 6.2e-51
Identity = 121/319 (37.93%), Postives = 176/319 (55.17%), Query Frame = 0


Query: 1   MSDAFCSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANES--GDNDPVR 60
           M +  C DCKR T +V DHS+GDT+CSECGLVLE+H I+ + EWRTFA++    D DP R
Sbjct: 1   MEEETCLDCKRPTIMVVDHSSGDTICSECGLVLEAHIIEYSQEWRTFASDDNHSDRDPNR 60

Query: 61  VGGPSNPLLADGGLSTVIAKPNGTAGEFLS----SSLGRWQNRGSNPDRGLI-LAFKTIA 120
           VG  +NP L  G L T+I KP  TA   LS    S+L R  N+  N +  LI  AF+ I 
Sbjct: 61  VGAATNPFLKSGDLVTIIEKPKETASSVLSKDDISTLFRAHNQVKNHEEDLIKQAFEEIQ 120

Query: 121 TMIG-------------------------RMRYIKELKIKSLVEEEIKMLYWLLAYTLLV 180
            M                           ++R  K+L                L+ TL  
Sbjct: 121 RMTDALDLDIVINSRACEIVSKYDGHANTKLRRGKKLNAICAASVSTACRELQLSRTLKE 180

Query: 181 DKKTSPAQSRKEIGRAKEYIVKQL--GLETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVK 240
             + +    +K+I R +  ++K++    +T  S     I+ G+ +RRFCS L ++ + + 
Sbjct: 181 IAEVANGVDKKDI-RKESLVIKRVLESHQTSVSASQAIINTGELVRRFCSKLDISQREIM 240

Query: 241 AAQEAVQKSEEFDIRRSPISIAAAVIYIITQLSD-DKKPLKDISVATGVAEGTIRNSYKD 285
           A  EAV+K+E FDIRR+P S+ AA+I++I+ +S  ++KP+++I +   V E TI+NS KD
Sbjct: 241 AIPEAVEKAENFDIRRNPKSVLAAIIFMISHISQTNRKPIREIGIVAEVVENTIKNSVKD 300

[Close]

BLAST of Carg21129 vs. TAIR 10
Match: AT3G57370.1 (Cyclin family protein )

HSP 1 Score: 109.8 bits (273), Expect = 3.8e-24
Identity = 102/362 (28.18%), Postives = 161/362 (44.48%), Query Frame = 0


Query: 6   CSDCKRQTEVVFDHSAGDTVCSECGLVLESHSIDETSEWRTFANESGDNDPVRVGGPSNP 65
           C  CK Q  VV DH    T C  CGL  +   I + S          +ND VR+  P+N 
Sbjct: 9   CRRCK-QINVVTDHVTRRTRCFGCGLEFKYRPIGDLS-------PVAENDTVRLPDPTNT 68

Query: 66  LLADGGLSTVI-------------------AKP-------------NGTAGEFLS----- 125
           LL++  LS V                    +KP             NG++ +FLS     
Sbjct: 69  LLSNTDLSIVTTEHKNGSFDDSLSLNLGNSSKPRLDPVSIATAKLMNGSSNDFLSLGTSQ 128

Query: 126 --------------SSLGRWQN--------RGSNPDRGLIL--------AFKTIATMIGR 185
                         S LG  Q           + P++ L +          K  AT+ G+
Sbjct: 129 NSETITASSDEFLFSDLGHLQKFSFDPLSMASTKPNKALSIVSIEAISNGLKLPATIKGQ 188

Query: 186 MRYIKELKIKSLVEEEIKMLYWLLAYTLLVDKKTSPAQSRKEIGR-AKEYIVKQLGLETG 245
              I ++       +E  +L+    Y    D   +  ++ +EI R A +  +  +    G
Sbjct: 189 ANEIFKVVESYARGKERNVLFAACIYIACRDNDMT--RTMREISRFANKASISDISETVG 248

Query: 246 ---QSVEMG-----TIHAGDFMRRFCSNLGMNNQAVKAAQEAVQKSEEF-DIRRSPISIA 291
              + +E+      +I   +F++RFCS   ++ +AV+AA EA +  +   + RR+P+S+A
Sbjct: 249 FIAEKLEINKNWYMSIETANFIKRFCSIFRLDKEAVEAALEAAESYDYMTNGRRAPVSVA 308

[Close]

BLAST of Carg21129 vs. TAIR 10
Match: AT4G36650.1 (plant-specific TFIIB-related protein )

HSP 1 Score: 64.3 bits (155), Expect = 1.8e-10
Identity = 45/125 (36.00%), Postives = 67/125 (53.60%), Query Frame = 0


Query: 158 RKEIGRAKEYIVKQLG--LETGQSVEMGTIHAGDFMRRFCSNLGMNNQAVKAAQE--AVQ 217
           +KEIG+     +K LG  L+  Q +   +I     M RFC+ L +N  A + A     V 
Sbjct: 204 QKEIGK----YIKILGEALQLSQPINSNSISV--HMPRFCTLLQLNKSAQELATHIGEVV 263

Query: 218 KSEEFDIRRSPISIAAAVIYIITQLSDDKKPLKDISVATGVAEGTIRNSYKDLYPHVSKI 277
            ++ F  RR+PISI+AA IY+  QL D +K   +I   TG+ E T+R  YK+L  +   +
Sbjct: 264 INKCFCTRRNPISISAAAIYLACQLEDKRKTQAEICKITGLTEVTLRKVYKELLENWDDL 322

Query: 278 IPGWY 279
           +P  Y
Sbjct: 324 LPSNY 322

The following BLAST results are available for this feature:

BLAST of Carg21129 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027639.1	1.1e-161	100.00	Transcription initiation factor IIB-2 [Cucurbita argyrosperma subsp. argyrosperm...	[more]
GAY66967.1	6.1e-149	92.83	hypothetical protein CUMW_253010 [Citrus unshiu]	[more]
XP_022924909.1	9.4e-134	82.69	transcription initiation factor IIB-2-like [Cucurbita moschata] >XP_023518635.1 ...	[more]
XP_022152227.1	4.7e-133	82.05	transcription initiation factor IIB-2 [Momordica charantia] >XP_022928717.1 tran...	[more]
XP_004150610.1	1.0e-132	81.73	transcription initiation factor IIB-2 [Cucumis sativus] >XP_008455781.1 PREDICTE...	[more]

BLAST of Carg21129 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SS44	3.6e-128	77.24	Transcription initiation factor IIB-2 OS=Arabidopsis thaliana OX=3702 GN=TFIIB2 ...	[more]
P48513	1.0e-127	78.59	Transcription initiation factor IIB OS=Glycine max OX=3847 GN=TFIIB1 PE=2 SV=1	[more]
Q8W0W3	3.1e-119	70.83	Transcription initiation factor IIB OS=Oryza sativa subsp. japonica OX=39947 GN=...	[more]
P48512	1.2e-115	70.19	Transcription initiation factor IIB-1 OS=Arabidopsis thaliana OX=3702 GN=TFIIB1 ...	[more]
Q9LIA6	8.8e-50	37.93	Plant-specific TFIIB-related protein 2 OS=Arabidopsis thaliana OX=3702 GN=PBRP2 ...	[more]

BLAST of Carg21129 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A2H5QQS2	2.9e-149	92.83	TFIIB-type domain-containing protein OS=Citrus unshiu OX=55188 GN=CUMW_253010 PE...	[more]
A0A6J1EAJ6	4.6e-134	82.69	transcription initiation factor IIB-2-like OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1HVL0	2.3e-133	82.05	transcription initiation factor IIB-2 OS=Cucurbita maxima OX=3661 GN=LOC11146723...	[more]
A0A6J1DFL4	2.3e-133	82.05	transcription initiation factor IIB-2 OS=Momordica charantia OX=3673 GN=LOC11101...	[more]
A0A6J1EKQ3	2.3e-133	82.05	transcription initiation factor IIB-2 OS=Cucurbita moschata OX=3662 GN=LOC111435...	[more]

BLAST of Carg21129 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G10330.1	2.6e-129	77.24	Cyclin-like family protein	[more]
AT2G41630.1	8.5e-117	70.19	transcription factor IIB	[more]
AT3G29380.1	6.2e-51	37.93	Cyclin-like family protein	[more]
AT3G57370.1	3.8e-24	28.18	Cyclin family protein	[more]
AT4G36650.1	1.8e-10	36.00	plant-specific TFIIB-related protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000812	Transcription factor TFIIB	PRINTS	PR00685	TIFACTORIIB	coord: 19..39 score: 54.6 coord: 222..238 score: 60.78 coord: 190..208 score: 47.37 coord: 55..76 score: 59.7 coord: 41..54 score: 50.0 coord: 254..268 score: 63.11
IPR000812	Transcription factor TFIIB	PANTHER	PTHR11618	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 3..126 coord: 156..290
IPR013763	Cyclin-like	SMART	SM00385	cyclin_7	coord: 189..268 e-value: 4.3E-7 score: 39.6
IPR013763	Cyclin-like	CDD	cd00043	CYCLIN	coord: 185..267 e-value: 2.46755E-9 score: 51.4705
IPR013137	Zinc finger, TFIIB-type	PFAM	PF08271	TF_Zn_Ribbon	coord: 6..47 e-value: 5.2E-15 score: 54.7
IPR013137	Zinc finger, TFIIB-type	PROSITE	PS51134	ZF_TFIIB	coord: 2..34 score: 9.105444
IPR013150	Transcription factor TFIIB, cyclin-like domain	PFAM	PF00382	TFIIB	coord: 191..259 e-value: 4.0E-9 score: 36.4
None	No IPR available	GENE3D	1.10.472.170		coord: 5..155 e-value: 2.4E-34 score: 120.9
None	No IPR available	GENE3D	1.10.472.10		coord: 186..289 e-value: 1.0E-32 score: 114.3
None	No IPR available	PANTHER	PTHR11618:SF69	TRANSCRIPTION INITIATION FACTOR IIB-RELATED	coord: 3..126 coord: 156..290
None	No IPR available	SUPERFAMILY	57783	Zinc beta-ribbon	coord: 4..50
IPR036915	Cyclin-like superfamily	SUPERFAMILY	47954	Cyclin-like	coord: 185..286

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21129-RA	Carg21129-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0070897	transcription preinitiation complex assembly
biological_process	GO:0006352	DNA-templated transcription, initiation
cellular_component	GO:0005634	nucleus
cellular_component	GO:0097550	transcription preinitiation complex
molecular_function	GO:0017025	TBP-class protein binding