Carg21121 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg21121
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Alpha carbonic anhydrase
Location	Carg_Chr07: 8045100 .. 8048499 (-)
RNA-Seq Expression	Carg21121
Synteny	Carg21121

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCTTCTTCTTCTTCAACAACAGACCTAATTATTTCCTTTTCTTTGCTGCTTGTTTCCTTCTCTCCTCCATTTCCAAGGCGTCTGATTCTGAAGTGGGTATGTTCTTTTTCTCTCCATTTTTTTCAAATCGTTCTTCGACGTTCAACCACACGACCACAACGTCACTACTTTTTAGTAACGATTCGATATAGACTTGAGCTTTACACATCTCTAATAACCATCGTTTATACATATGTATTTCCTTTTGAGTCGTTGGCTTCGTTCTAATGAGAAATGGGTATTGTCGAGTACGATTAGAGTTGAGGTAAATCACAAGGAAATAAAAAACAAAGTCATCCCGAGAGGGTTTATGCTTGATAGTACCCTATTGTAAGTTGTCATGAATAATAGGTTCATGTATCTCCTATGCATGTATTGACATTTATTTAGTTCATAATTCTCATTTATTCGGTTCATAACCCTTTTGTTCATCTTTCATTTTGATACATGAATTATGTGTAAACACTTTTGTCATTTTGCCTAGAAACAATCGGGAGGAACTTTGTAAGAGAGACTCTGTAGCTAAGAGTGTGAGAGATACATTTGTAAACACTTTGATTATAGCGATTGGTTGCCACCCAGTGGATATAAGGGAATTTCTTGTTTCCGAACCACATAAATCTTTGTGTCTCTTTGTTTAATCTTTGTTCGTGGGTATGTGAGTACAATCGATCTTGCTTTCGCTCCCAGTTCGTTACTACAACCCCAAGGAATGATGTTTTACCAGATGTCTTCCTGTGAGGTCGAGTTCATGGCAACGACTACAGTTAAAGAAGAGATTGTGAATCAGTAACCTCGACCTAACATTTGTAGAAGAAGTTACTAATCGTAACGCGTTTATCGAACAGATGATGAATCTGCATTTACTTACTACGAGGGAACTGGAAGAGGGCCAAGGAGCTGGTGGAAGCTGAATCCAGAATGGGAGTCATGTGGGAAGGGGAAGTATCAGTCTCCCATCAACATTGTGGAAGAAGAAGTGGGCGTTCTTCCTGAGCTTGGGAAGCTCCAAAGAGACTACACCCCAGCTCCGGCGCTGATCAAGAATCGGGGGCACGACATGACGGTACGGTGCTACGTTAGAGTGGTTAGTGTCTAAGCTCGCCTCTAATGGTGTTTGTTTTGATATTATGTTCAGATAAGCTGGAAACAAGATGCTGGGAAGATTACTATCAATGGGACTCGTTACAGCTTGATCCAATGCCATTGGCATACCCCTTCTGAGCACTCTTTCAATGGAACAAGGTTTCTCTCTCTCTCTCTCTCACTCTCTTTTAGGGTTCTATTCACGAAAGGGTGTTTGACTCGTGGGTTGGGTTAGGTTTGTGTAGCTCGAAGTTGAGTAATTGAGTCTATGGTGGTATTCGAGTACGACTCGAATTTCACGGCTTTTCTGAAGCCTAACTCGAGTCTATGATGGCATTCAAATACGACTCGAATATTATGGCTTTTTTGAAGTTTAACTCGAGTCTATGATGGCATTCGAGTACGATTCGAATTTTCCAGCTTTTTTGAAGCTTAACTCGAGTCTATAATGGCATTTGAGTATGACTCGAATCTTACGGCTTTTCTGAAGGTTATTATCACATGTGTGCTAGTAGATATTCAAATCGAGATAAACTAGAACTTAGTAGACCTATAACAAAGGTGGAAATCAAACTAACATAGACCATATGGCTTATTAGTAAATGCACGGTCGCTCACCCGCATATCCTCATCGTTGCCTTAAAAAAAAAATTGTTGAGTATTAAAAATAGGTTAAATTAATAAAAATATCCTTAAACTTTATATTATGTCTCAAAATTACTCATAAACTTTCAAAAGTTTCAATAATATCCTTAAACTTTTATAAAACAATTTAAAAAAATACCGGTTCGTTTTGGATGAAAATGTCTTTTTAACTTTTAAAAAGTTTCAAAATATCTTTTTTAACTCTTAAAAGTATTTAAAAAATATCCAAAAGTTTTCTTAATATTTTTTAAAAAATTAAAATATTTTAAATTAAAAAAAATATCGTTACCATACCCGTTTCAAAAACAATTTTTGTCCATATATTGATGGTAAGAATTTTTTTGAACTTCTTCCCGGTGGTAATGCTATTTTTAAAATTTCAATACTCACGATAAGATTATCTCTAAACTTTTTTTTAAAAAAAATTAAATAATATTAATAAAACTTTTTAAAGTATAAAGGTATTTTTGAGAGACGGTTAAAAGTTTAAAGGTATTTTTGAAATAAAATACTAGCGGTTTCCATCCAATACTAACGACAAGGGTATTTTTGTCTTTTTTGAAAATTTAAGGGTATTATTGAAACTTTTGAAGGTTTATGAGTATTTTTTAGACAAAGTACTAAATTTATCGGTATTTTTTTTTATTAATTTAGCCTTAAAAATATTCGACAAGTAACCCTCCTCATAGCAATATGTCATTCACACATATTCTAGCATGCAAACACATACCACCCGACAAACTATACCTATTTAAACTTACCTCAAAGCAAAAACTAAACCAACAAGAAACTTCGTCTCAATAGACTCTTGGCGACATGACCGAGCAACAACGTCGAGTGAAGGGTTGAGGAAGGTCCGAGTGGCTAATTCTTTTTAACCTTATCCTAATCGACCTTAACTCCGCTAACTAACTTAAGATAAGGGCATGACTCTGATACTCTTAAAAGATCTCGTAGTAACGGAAATAGCGTCTCTTACTTAGATTCTTTTCGATTAGTCAATATAGAACCCGAACATGGTAATGGTCGGGATTTATAAGACGTAATATCTATATGTTATGAAACTTTGCAGACATGTTCTAGAGTTGCATATGGTTCATCAAAGCCAAGATAATAAGACCGCAGTTATTGGAATTCTGTACAAATATGGACGACCTGATCCCTTCCTTTCAAGGGTATCTCTTACTTTCTTAAAATCATTTCAATTTTGTTGGGTTTTGGTCATTTTGATTTTGATTATTGAAGATTTTCATGAGATTTGTTGTTTGTGTGTGAAGCTGTTTCATCACATCAAATCAATTGGGAAAGAAGAAAAGGAAATTGGGATTGTGAATCCCGGAGACATCAAGTTCGGGAGCAGAAAATATTACCGATATATTGGCTCTCTGACGGTTCCTCCGTGTAGCGAAGGTGTCGTTTGGACGATACTCAAGAAGGTTTCTTCGTACTCACTTTCTTTCGTAACGTTACGAACACTAAATTGTTGTAAACTAAAGTTTGTGAACAAATGAACTAAGCGTCGTTGAATTGGTATAGGTGAGAACAGTTTCACGAGATCAAGTGAGAGTTTTGGCTAATGCTATTCACGATGTGAGTATTTTCTGTTCTTTTGTTCTTCGGATTTGA

mRNA sequence

ATGGCCTTCTTCTTCTTCAACAACAGACCTAATTATTTCCTTTTCTTTGCTGCTTGTTTCCTTCTCTCCTCCATTTCCAAGGCGTCTGATTCTGAAGTGGATGATGAATCTGCATTTACTTACTACGAGGGAACTGGAAGAGGGCCAAGGAGCTGGTGGAAGCTGAATCCAGAATGGGAGTCATGTGGGAAGGGGAAGTATCAGTCTCCCATCAACATTGTGGAAGAAGAAGTGGGCGTTCTTCCTGAGCTTGGGAAGCTCCAAAGAGACTACACCCCAGCTCCGGCGCTGATCAAGAATCGGGGGCACGACATGACGATAAGCTGGAAACAAGATGCTGGGAAGATTACTATCAATGGGACTCGTTACAGCTTGATCCAATGCCATTGGCATACCCCTTCTGAGCACTCTTTCAATGGAACAAGACATGTTCTAGAGTTGCATATGGTTCATCAAAGCCAAGATAATAAGACCGCAGTTATTGGAATTCTGTACAAATATGGACGACCTGATCCCTTCCTTTCAAGGCTGTTTCATCACATCAAATCAATTGGGAAAGAAGAAAAGGAAATTGGGATTGTGAATCCCGGAGACATCAAGTTCGGGAGCAGAAAATATTACCGATATATTGGCTCTCTGACGGTTCCTCCGTGTAGCGAAGGTGTCGTTTGGACGATACTCAAGAAGGTGAGAACAGTTTCACGAGATCAAGTGAGAGTTTTGGCTAATGCTATTCACGATGTGAGTATTTTCTGTTCTTTTGTTCTTCGGATTTGA

Coding sequence (CDS)

ATGGCCTTCTTCTTCTTCAACAACAGACCTAATTATTTCCTTTTCTTTGCTGCTTGTTTCCTTCTCTCCTCCATTTCCAAGGCGTCTGATTCTGAAGTGGATGATGAATCTGCATTTACTTACTACGAGGGAACTGGAAGAGGGCCAAGGAGCTGGTGGAAGCTGAATCCAGAATGGGAGTCATGTGGGAAGGGGAAGTATCAGTCTCCCATCAACATTGTGGAAGAAGAAGTGGGCGTTCTTCCTGAGCTTGGGAAGCTCCAAAGAGACTACACCCCAGCTCCGGCGCTGATCAAGAATCGGGGGCACGACATGACGATAAGCTGGAAACAAGATGCTGGGAAGATTACTATCAATGGGACTCGTTACAGCTTGATCCAATGCCATTGGCATACCCCTTCTGAGCACTCTTTCAATGGAACAAGACATGTTCTAGAGTTGCATATGGTTCATCAAAGCCAAGATAATAAGACCGCAGTTATTGGAATTCTGTACAAATATGGACGACCTGATCCCTTCCTTTCAAGGCTGTTTCATCACATCAAATCAATTGGGAAAGAAGAAAAGGAAATTGGGATTGTGAATCCCGGAGACATCAAGTTCGGGAGCAGAAAATATTACCGATATATTGGCTCTCTGACGGTTCCTCCGTGTAGCGAAGGTGTCGTTTGGACGATACTCAAGAAGGTGAGAACAGTTTCACGAGATCAAGTGAGAGTTTTGGCTAATGCTATTCACGATGTGAGTATTTTCTGTTCTTTTGTTCTTCGGATTTGA

Protein sequence

MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIHDVSIFCSFVLRI

Homology

[Close]

BLAST of Carg21121 vs. NCBI nr
Match: KAG7027647.1 (Alpha carbonic anhydrase 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 540.4 bits (1391), Expect = 8.3e-150
Identity = 258/258 (100.00%), Postives = 258/258 (100.00%), Query Frame = 0


Query: 1   MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE 60
           MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE
Sbjct: 1   MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE 60

Query: 61  SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING 120
           SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING
Sbjct: 61  SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING 120

Query: 121 TRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH 180
           TRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH
Sbjct: 121 TRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH 180

Query: 181 IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV 240
           IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV
Sbjct: 181 IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV 240

Query: 241 LANAIHDVSIFCSFVLRI 259
           LANAIHDVSIFCSFVLRI
Sbjct: 241 LANAIHDVSIFCSFVLRI 258

[Close]

BLAST of Carg21121 vs. NCBI nr
Match: KAG6595685.1 (Alpha carbonic anhydrase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 534.3 bits (1375), Expect = 6.0e-148
Identity = 256/258 (99.22%), Postives = 256/258 (99.22%), Query Frame = 0


Query: 1   MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE 60
           MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE
Sbjct: 1   MAFFFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE 60

Query: 61  SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING 120
           SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING
Sbjct: 61  SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING 120

Query: 121 TRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH 180
           TRY LIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH
Sbjct: 121 TRYRLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH 180

Query: 181 IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV 240
           IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV
Sbjct: 181 IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV 240

Query: 241 LANAIHDVSIFCSFVLRI 259
           LANAIHDVSIF SFVLRI
Sbjct: 241 LANAIHDVSIFYSFVLRI 258

[Close]

BLAST of Carg21121 vs. NCBI nr
Match: XP_022966427.1 (alpha carbonic anhydrase 4-like [Cucurbita maxima])

HSP 1 Score: 489.6 bits (1259), Expect = 1.7e-134
Identity = 232/244 (95.08%), Postives = 238/244 (97.54%), Query Frame = 0


Query: 4   FFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCG 63
           FFFNNRPNYFLFFAA FLLSSISKAS SEVDDESAFTYYEGTG+GP+SWWKLNP+WESCG
Sbjct: 3   FFFNNRPNYFLFFAAFFLLSSISKASGSEVDDESAFTYYEGTGKGPKSWWKLNPKWESCG 62

Query: 64  KGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRY 123
           KGKYQSPINIVEEEV VLPELGKLQRDY PAPALIKNRGHDMTISWKQDAGKITINGTRY
Sbjct: 63  KGKYQSPINIVEEEVVVLPELGKLQRDYNPAPALIKNRGHDMTISWKQDAGKITINGTRY 122

Query: 124 SLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKS 183
            LIQCHWHT SEHSFNGTRHVLELHMVHQSQDNKTAVIGI+YKYGRPDPFLSRLFHHIKS
Sbjct: 123 RLIQCHWHTLSEHSFNGTRHVLELHMVHQSQDNKTAVIGIVYKYGRPDPFLSRLFHHIKS 182

Query: 184 IGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLAN 243
           IGK+EKEIGIVNPGDIKFG+RKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLAN
Sbjct: 183 IGKKEKEIGIVNPGDIKFGNRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLAN 242

Query: 244 AIHD 248
           AIHD
Sbjct: 243 AIHD 246

[Close]

BLAST of Carg21121 vs. NCBI nr
Match: XP_023518783.1 (LOW QUALITY PROTEIN: alpha carbonic anhydrase 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 418.7 bits (1075), Expect = 3.7e-113
Identity = 195/201 (97.01%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 47  RGPRSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMT 106
           +G RSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDY PA ALIKNRGHDMT
Sbjct: 13  KGXRSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYNPARALIKNRGHDMT 72

Query: 107 ISWKQDAGKITINGTRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK 166
           ISWKQDAGKITINGTRY LIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK
Sbjct: 73  ISWKQDAGKITINGTRYRLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK 132

Query: 167 YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI 226
           YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFG+RKYYRYIGSLTVPPCSEGVVWTI
Sbjct: 133 YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGTRKYYRYIGSLTVPPCSEGVVWTI 192

Query: 227 LKKVRTVSRDQVRVLANAIHD 248
           LKKVRTVSRDQVRVLANAIHD
Sbjct: 193 LKKVRTVSRDQVRVLANAIHD 213

[Close]

BLAST of Carg21121 vs. NCBI nr
Match: XP_022925105.1 (LOW QUALITY PROTEIN: alpha carbonic anhydrase 4-like [Cucurbita moschata])

HSP 1 Score: 418.7 bits (1075), Expect = 3.7e-113
Identity = 195/201 (97.01%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 47  RGPRSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMT 106
           +G +SWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDY PA ALIKNRGHDMT
Sbjct: 13  KGXKSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYNPARALIKNRGHDMT 72

Query: 107 ISWKQDAGKITINGTRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK 166
           ISWKQDAGKITINGTRY LIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK
Sbjct: 73  ISWKQDAGKITINGTRYRLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK 132

Query: 167 YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI 226
           YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI
Sbjct: 133 YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI 192

Query: 227 LKKVRTVSRDQVRVLANAIHD 248
           LKKVRTVSRDQVRVLANAIHD
Sbjct: 193 LKKVRTVSRDQVRVLANAIHD 213

[Close]

BLAST of Carg21121 vs. ExPASy Swiss-Prot
Match: F4JIK2 (Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 1.6e-74
Identity = 124/235 (52.77%), Postives = 172/235 (73.19%), Query Frame = 0


Query: 15  FFAACFLLSSISKAS--DSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPIN 74
           F A CF+  S    S   SEVDDE+ FTY + T +GP  W K+NP W+ C  G+YQSPI+
Sbjct: 10  FMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPID 69

Query: 75  IVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCHWHT 134
           +  E V ++ +     R Y PAPA+I NRGHD+ +SWK DAGK+TI  T ++L+QCHWH+
Sbjct: 70  LTNERVSLIHDQA-WTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHS 129

Query: 135 PSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIGKEEKEIG 194
           PSEH+ NGTR+ LELHMVH S   +TAVIG+LYK G P+ FL++L + IK++G +E  +G
Sbjct: 130 PSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGNKEINLG 189

Query: 195 IVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIHD 248
           +++P +I+F +RK+YRYIGSLTVPPC+EGV+WT++K+V T+S +Q+  L  A+ D
Sbjct: 190 MIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDD 243

[Close]

BLAST of Carg21121 vs. ExPASy Swiss-Prot
Match: Q8L817 (Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 1.8e-67
Identity = 117/241 (48.55%), Postives = 165/241 (68.46%), Query Frame = 0


Query: 12  YFLFFAACFLLSSISKASDS--EVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQS 71
           +F+   + F + SIS A+ S  EV+DE  F Y +   +GP  W +L PEWE CGKG+ QS
Sbjct: 11  FFVALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQS 70

Query: 72  PINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCH 131
           PI+++ E V ++  LG+L RDY P+ A +KNRGHD+ + ++  AG I ING  Y L Q H
Sbjct: 71  PIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLH 130

Query: 132 WHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIG---K 191
           WH+PSEH+ NG R  LELHMVH+ ++ + AV+ +LYK GR D F+  L   ++ I    +
Sbjct: 131 WHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEE 190

Query: 192 EEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIH 248
            EK +G+++P  IK GSRKYYRY GSLT PPC++ V W++++KVRTV+R QV++L  A+H
Sbjct: 191 AEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVH 250

[Close]

BLAST of Carg21121 vs. ExPASy Swiss-Prot
Match: Q9SUB4 (Alpha carbonic anhydrase 6 OS=Arabidopsis thaliana OX=3702 GN=ACA6 PE=3 SV=1)

HSP 1 Score: 247.3 bits (630), Expect = 1.9e-64
Identity = 113/244 (46.31%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 12  YFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPI 71
           +F+ F       +I      E+ ++  FTY + T +GP  W KL+P+W+ C  GK QSPI
Sbjct: 10  FFVVFFIDLFSPNILFVYAREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPI 69

Query: 72  NIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCHWH 131
           ++ +E V ++ +   L + Y PA A+I++RGHD+ +SWK D GKITI+ T Y L+QCHWH
Sbjct: 70  DLTDERVSLIHDQA-LSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHWH 129

Query: 132 TPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIGKEEKEI 191
           +PSEH+ NGT + LELHMVH S   KT V+G+LYK G PD FL+++ + IK +GK+E ++
Sbjct: 130 SPSEHTINGTSYDLELHMVHTSASGKTTVVGVLYKLGEPDEFLTKILNGIKGVGKKEIDL 189

Query: 192 GIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIHDVSIF 251
           GIV+P DI+F +  +YRYIGSLT+PPC+EGV+WT+ K+V        R++      ++IF
Sbjct: 190 GIVDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTVQKRVLYFFCFCYRLIIFVTPYINIF 249

Query: 252 CSFV 256
             FV
Sbjct: 250 WIFV 252

[Close]

BLAST of Carg21121 vs. ExPASy Swiss-Prot
Match: F4IHR4 (Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2)

HSP 1 Score: 235.7 bits (600), Expect = 5.8e-61
Identity = 108/216 (50.00%), Postives = 150/216 (69.44%), Query Frame = 0


Query: 22  LSSISKASD-SEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPINIVEEEVGV 81
           +S +S A+D  EV+DE  F+Y      GP  W KL PEW+ CGKG+ QSPI+++ + V +
Sbjct: 21  VSCLSAATDYREVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRL 80

Query: 82  LPELGKLQRDYTPAPALIKNRGHDMTISW-KQDAGKITINGTRYSLIQCHWHTPSEHSFN 141
           +  L KL R Y P  A +KNRGHDM + + ++ +G IT+NGT Y L+Q HWH+PSEH+ N
Sbjct: 81  VTHLKKLTRHYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMN 140

Query: 142 GTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSI---GKEEKEIGIVNP 201
           G R  LELHMVH++ +   AV+ +LYK GRPD FL  L + + +I    + EK + +++P
Sbjct: 141 GRRFALELHMVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDP 200

Query: 202 GDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRT 233
            DIK GSRK+YRYIGSLT PPC++ V+WT++KKV T
Sbjct: 201 RDIKIGSRKFYRYIGSLTTPPCTQNVIWTVVKKVNT 236

[Close]

BLAST of Carg21121 vs. ExPASy Swiss-Prot
Match: Q84UV8 (Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS=Nicotiana langsdorffii x Nicotiana sanderae OX=164110 GN=NEC3 PE=1 SV=1)

HSP 1 Score: 230.3 bits (586), Expect = 2.4e-59
Identity = 114/238 (47.90%), Postives = 156/238 (65.55%), Query Frame = 0


Query: 14  LFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPINI 73
           + F +   LSS+  A   EVDDES F+Y E +  GP +W  + P+W+ C  GK QSPI+I
Sbjct: 9   MLFISFLFLSSVFLARSGEVDDESEFSYDEKSENGPANWGNIRPDWKEC-SGKLQSPIDI 68

Query: 74  VEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCHWHTP 133
            +    V+  L  LQ+DY P+ A + NRGHD+ +    D G + IN T+Y L Q HWHTP
Sbjct: 69  FDLRAEVVSNLRILQKDYKPSNATLLNRGHDIMLR-LDDGGYLKINETQYQLKQLHWHTP 128

Query: 134 SEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGR-PDPFLSRLFHHIKSIGKE---EK 193
           SEH+ NG R  LE H+VH+S + K  VIGI+Y+ G  PDPFLS + + +K    +   E+
Sbjct: 129 SEHTINGERFNLEAHLVHESNNGKFVVIGIVYEIGLWPDPFLSMIENDLKVPANKKGIER 188

Query: 194 EIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIHD 248
            IGI++P  IK   +KY+RYIGSLT PPC+EGVVW I +KV+TV+R Q+++L  A+HD
Sbjct: 189 GIGIIDPNQIKLDGKKYFRYIGSLTTPPCTEGVVWIIDRKVKTVTRRQIKLLQEAVHD 244

[Close]

BLAST of Carg21121 vs. ExPASy TrEMBL
Match: A0A6J1HMY4 (alpha carbonic anhydrase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111466081 PE=3 SV=1)

HSP 1 Score: 489.6 bits (1259), Expect = 8.2e-135
Identity = 232/244 (95.08%), Postives = 238/244 (97.54%), Query Frame = 0


Query: 4   FFFNNRPNYFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCG 63
           FFFNNRPNYFLFFAA FLLSSISKAS SEVDDESAFTYYEGTG+GP+SWWKLNP+WESCG
Sbjct: 3   FFFNNRPNYFLFFAAFFLLSSISKASGSEVDDESAFTYYEGTGKGPKSWWKLNPKWESCG 62

Query: 64  KGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRY 123
           KGKYQSPINIVEEEV VLPELGKLQRDY PAPALIKNRGHDMTISWKQDAGKITINGTRY
Sbjct: 63  KGKYQSPINIVEEEVVVLPELGKLQRDYNPAPALIKNRGHDMTISWKQDAGKITINGTRY 122

Query: 124 SLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKS 183
            LIQCHWHT SEHSFNGTRHVLELHMVHQSQDNKTAVIGI+YKYGRPDPFLSRLFHHIKS
Sbjct: 123 RLIQCHWHTLSEHSFNGTRHVLELHMVHQSQDNKTAVIGIVYKYGRPDPFLSRLFHHIKS 182

Query: 184 IGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLAN 243
           IGK+EKEIGIVNPGDIKFG+RKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLAN
Sbjct: 183 IGKKEKEIGIVNPGDIKFGNRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLAN 242

Query: 244 AIHD 248
           AIHD
Sbjct: 243 AIHD 246

[Close]

BLAST of Carg21121 vs. ExPASy TrEMBL
Match: A0A6J1EGY9 (LOW QUALITY PROTEIN: alpha carbonic anhydrase 4-like OS=Cucurbita moschata OX=3662 GN=LOC111432446 PE=3 SV=1)

HSP 1 Score: 418.7 bits (1075), Expect = 1.8e-113
Identity = 195/201 (97.01%), Postives = 197/201 (98.01%), Query Frame = 0


Query: 47  RGPRSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMT 106
           +G +SWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDY PA ALIKNRGHDMT
Sbjct: 13  KGXKSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQRDYNPARALIKNRGHDMT 72

Query: 107 ISWKQDAGKITINGTRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK 166
           ISWKQDAGKITINGTRY LIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK
Sbjct: 73  ISWKQDAGKITINGTRYRLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYK 132

Query: 167 YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI 226
           YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI
Sbjct: 133 YGRPDPFLSRLFHHIKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTI 192

Query: 227 LKKVRTVSRDQVRVLANAIHD 248
           LKKVRTVSRDQVRVLANAIHD
Sbjct: 193 LKKVRTVSRDQVRVLANAIHD 213

[Close]

BLAST of Carg21121 vs. ExPASy TrEMBL
Match: A0A7N2M2N8 (Alpha-carbonic anhydrase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 356.3 bits (913), Expect = 1.1e-94
Identity = 162/247 (65.59%), Postives = 197/247 (79.76%), Query Frame = 0


Query: 4   FFFNNRPNYFLFFAACFLLS---SISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE 63
           FF  N    FLF  +  L S   S S ASDSEVDDE+ FTY EGTG+GP+ W +++P W+
Sbjct: 3   FFHTNPITIFLFLTSLILSSHPYSFSAASDSEVDDETPFTYTEGTGKGPKKWGQIDPHWK 62

Query: 64  SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING 123
           +CG GK QSPI+++   V V P LGKL+RDY PAPA++KNRGHD+T+ WK DAGKI ING
Sbjct: 63  ACGNGKMQSPIDLLNRRVQVFPSLGKLKRDYQPAPAVVKNRGHDITVMWKGDAGKINING 122

Query: 124 TRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH 183
           T Y L+Q HWH+PSEH+FNG+R+ LELH+VH S   + AV+GI+YKYG PDP LS+LFHH
Sbjct: 123 TDYKLLQFHWHSPSEHTFNGSRYDLELHIVHLSSSGEIAVVGIVYKYGHPDPLLSKLFHH 182

Query: 184 IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV 243
           IKS+GKEE+ +GI NPG IKFGSRKYYRYIGSLTVPPC+EGV+WTI+KKVRTVSR+QVR 
Sbjct: 183 IKSVGKEERVLGIFNPGAIKFGSRKYYRYIGSLTVPPCTEGVIWTIIKKVRTVSREQVRA 242

Query: 244 LANAIHD 248
           L  A+HD
Sbjct: 243 LREAVHD 249

[Close]

BLAST of Carg21121 vs. ExPASy TrEMBL
Match: A0A7N2M0S3 (Alpha-carbonic anhydrase domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 355.9 bits (912), Expect = 1.4e-94
Identity = 162/247 (65.59%), Postives = 198/247 (80.16%), Query Frame = 0


Query: 4   FFFNNRPNYFLFFAACFLLS---SISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWE 63
           FF  N    FLF  +  L S   + S ASDSEVDDE  FTY EGTG+GP+ W +++P W+
Sbjct: 3   FFHINPITIFLFLTSLILSSHPYNFSAASDSEVDDEIPFTYTEGTGKGPKKWGQIDPRWK 62

Query: 64  SCGKGKYQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITING 123
           +CG GK QSPI+++   V V P LGKL+RDY PAPA++KNRGHD+T+ WK DAGKI ING
Sbjct: 63  ACGNGKMQSPIDLLNRRVQVFPSLGKLKRDYQPAPAVVKNRGHDITVMWKGDAGKINING 122

Query: 124 TRYSLIQCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHH 183
           T Y L+Q HWH+PSEH+FNG+R+ LELH+VH S   + AV+GI+YKYGRPDP LS+LFHH
Sbjct: 123 TDYKLLQFHWHSPSEHTFNGSRYDLELHIVHLSSSGEIAVVGIVYKYGRPDPLLSKLFHH 182

Query: 184 IKSIGKEEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRV 243
           IKS+GKEE+++GI NPG IKFGSRKYYRYIGSLTVPPC+EGV+WTI+KKVRTVSR+QVR 
Sbjct: 183 IKSVGKEERDLGIFNPGAIKFGSRKYYRYIGSLTVPPCTEGVIWTIVKKVRTVSREQVRA 242

Query: 244 LANAIHD 248
           L  A+HD
Sbjct: 243 LREAVHD 249

[Close]

BLAST of Carg21121 vs. ExPASy TrEMBL
Match: V4RJW3 (Alpha-carbonic anhydrase domain-containing protein OS=Citrus clementina OX=85681 GN=CICLE_v10005610mg PE=3 SV=1)

HSP 1 Score: 355.5 bits (911), Expect = 1.8e-94
Identity = 161/241 (66.80%), Postives = 194/241 (80.50%), Query Frame = 0


Query: 11  NYFLFFAACFLLSS----ISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGK 70
           N+     A  +LSS    +  A DSEVDDE+AF+Y E TG+GPR W K+NP W  CG GK
Sbjct: 6   NFTFIILASLILSSHLYNLVNADDSEVDDETAFSYKEETGKGPREWGKINPHWRVCGIGK 65

Query: 71  YQSPINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLI 130
            QSPI+++ + V VLP LGKL+RDY PAPA++KNRGHD+ + W+ DAGK+ INGT Y L+
Sbjct: 66  LQSPIDLLNQRVQVLPALGKLKRDYKPAPAVVKNRGHDICVWWRGDAGKMNINGTVYKLL 125

Query: 131 QCHWHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIGK 190
           QCHWHTPSEH+FNG+ + +ELH+VH S D K AV GI+Y+YGRPDPFLS+L HHIKS+GK
Sbjct: 126 QCHWHTPSEHTFNGSSYEMELHLVHLSSDGKLAVTGIVYEYGRPDPFLSKLLHHIKSLGK 185

Query: 191 EEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIH 248
           EEKE+GIVNPGDIKFGSRKYYRYIGSLTVPPC+EGV+WTI KKVRTVSR+QVR L   +H
Sbjct: 186 EEKEVGIVNPGDIKFGSRKYYRYIGSLTVPPCTEGVIWTIGKKVRTVSREQVRALKEEVH 245

[Close]

BLAST of Carg21121 vs. TAIR 10
Match: AT4G20990.1 (alpha carbonic anhydrase 4 )

HSP 1 Score: 280.8 bits (717), Expect = 1.1e-75
Identity = 124/235 (52.77%), Postives = 172/235 (73.19%), Query Frame = 0


Query: 15  FFAACFLLSSISKAS--DSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPIN 74
           F A CF+  S    S   SEVDDE+ FTY + T +GP  W K+NP W+ C  G+YQSPI+
Sbjct: 10  FMAMCFIYLSFPNISHAHSEVDDETPFTYEQKTEKGPEGWGKINPHWKVCNTGRYQSPID 69

Query: 75  IVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCHWHT 134
           +  E V ++ +     R Y PAPA+I NRGHD+ +SWK DAGK+TI  T ++L+QCHWH+
Sbjct: 70  LTNERVSLIHDQA-WTRQYKPAPAVITNRGHDIMVSWKGDAGKMTIRKTDFNLVQCHWHS 129

Query: 135 PSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIGKEEKEIG 194
           PSEH+ NGTR+ LELHMVH S   +TAVIG+LYK G P+ FL++L + IK++G +E  +G
Sbjct: 130 PSEHTVNGTRYDLELHMVHTSARGRTAVIGVLYKLGEPNEFLTKLLNGIKAVGNKEINLG 189

Query: 195 IVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIHD 248
           +++P +I+F +RK+YRYIGSLTVPPC+EGV+WT++K+V T+S +Q+  L  A+ D
Sbjct: 190 MIDPREIRFQTRKFYRYIGSLTVPPCTEGVIWTVVKRVNTISMEQITALRQAVDD 243

[Close]

BLAST of Carg21121 vs. TAIR 10
Match: AT1G08080.1 (alpha carbonic anhydrase 7 )

HSP 1 Score: 257.3 bits (656), Expect = 1.3e-68
Identity = 117/241 (48.55%), Postives = 165/241 (68.46%), Query Frame = 0


Query: 12  YFLFFAACFLLSSISKASDS--EVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQS 71
           +F+   + F + SIS A+ S  EV+DE  F Y +   +GP  W +L PEWE CGKG+ QS
Sbjct: 11  FFVALFSIFTIVSISSAASSHGEVEDEREFNYKKNDEKGPERWGELKPEWEMCGKGEMQS 70

Query: 72  PINIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCH 131
           PI+++ E V ++  LG+L RDY P+ A +KNRGHD+ + ++  AG I ING  Y L Q H
Sbjct: 71  PIDLMNERVNIVSHLGRLNRDYNPSNATLKNRGHDIMLKFEDGAGTIKINGFEYELQQLH 130

Query: 132 WHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIG---K 191
           WH+PSEH+ NG R  LELHMVH+ ++ + AV+ +LYK GR D F+  L   ++ I    +
Sbjct: 131 WHSPSEHTINGRRFALELHMVHEGRNRRMAVVTVLYKIGRADTFIRSLEKELEGIAEMEE 190

Query: 192 EEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIH 248
            EK +G+++P  IK GSRKYYRY GSLT PPC++ V W++++KVRTV+R QV++L  A+H
Sbjct: 191 AEKNVGMIDPTKIKIGSRKYYRYTGSLTTPPCTQNVTWSVVRKVRTVTRKQVKLLRVAVH 250

[Close]

BLAST of Carg21121 vs. TAIR 10
Match: AT4G21000.1 (alpha carbonic anhydrase 6 )

HSP 1 Score: 247.3 bits (630), Expect = 1.4e-65
Identity = 113/244 (46.31%), Postives = 164/244 (67.21%), Query Frame = 0


Query: 12  YFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPI 71
           +F+ F       +I      E+ ++  FTY + T +GP  W KL+P+W+ C  GK QSPI
Sbjct: 10  FFVVFFIDLFSPNILFVYAREIGNKPLFTYKQKTEKGPAEWGKLDPQWKVCSTGKIQSPI 69

Query: 72  NIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWKQDAGKITINGTRYSLIQCHWH 131
           ++ +E V ++ +   L + Y PA A+I++RGHD+ +SWK D GKITI+ T Y L+QCHWH
Sbjct: 70  DLTDERVSLIHDQA-LSKHYKPASAVIQSRGHDVMVSWKGDGGKITIHQTDYKLVQCHWH 129

Query: 132 TPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSIGKEEKEI 191
           +PSEH+ NGT + LELHMVH S   KT V+G+LYK G PD FL+++ + IK +GK+E ++
Sbjct: 130 SPSEHTINGTSYDLELHMVHTSASGKTTVVGVLYKLGEPDEFLTKILNGIKGVGKKEIDL 189

Query: 192 GIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIHDVSIF 251
           GIV+P DI+F +  +YRYIGSLT+PPC+EGV+WT+ K+V        R++      ++IF
Sbjct: 190 GIVDPRDIRFETNNFYRYIGSLTIPPCTEGVIWTVQKRVLYFFCFCYRLIIFVTPYINIF 249

Query: 252 CSFV 256
             FV
Sbjct: 250 WIFV 252

[Close]

BLAST of Carg21121 vs. TAIR 10
Match: AT2G28210.1 (alpha carbonic anhydrase 2 )

HSP 1 Score: 232.6 bits (592), Expect = 3.5e-61
Identity = 104/207 (50.24%), Postives = 143/207 (69.08%), Query Frame = 0


Query: 30  DSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPINIVEEEVGVLPELGKLQR 89
           D  V+DE  F+Y      GP  W KL PEW+ CGKG+ QSPI+++ + V ++  L KL R
Sbjct: 2   DEYVEDEHEFSYEWNQENGPAKWGKLRPEWKMCGKGEMQSPIDLMNKRVRLVTHLKKLTR 61

Query: 90  DYTPAPALIKNRGHDMTISW-KQDAGKITINGTRYSLIQCHWHTPSEHSFNGTRHVLELH 149
            Y P  A +KNRGHDM + + ++ +G IT+NGT Y L+Q HWH+PSEH+ NG R  LELH
Sbjct: 62  HYKPCNATLKNRGHDMMLKFGEEGSGSITVNGTEYKLLQLHWHSPSEHTMNGRRFALELH 121

Query: 150 MVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSI---GKEEKEIGIVNPGDIKFGSRK 209
           MVH++ +   AV+ +LYK GRPD FL  L + + +I    + EK + +++P DIK GSRK
Sbjct: 122 MVHENINGSLAVVTVLYKIGRPDSFLGLLENKLSAITDQNEAEKYVDVIDPRDIKIGSRK 181

Query: 210 YYRYIGSLTVPPCSEGVVWTILKKVRT 233
           +YRYIGSLT PPC++ V+WT++KKV T
Sbjct: 182 FYRYIGSLTTPPCTQNVIWTVVKKVNT 208

[Close]

BLAST of Carg21121 vs. TAIR 10
Match: AT1G08065.1 (alpha carbonic anhydrase 5 )

HSP 1 Score: 223.8 bits (569), Expect = 1.6e-58
Identity = 113/243 (46.50%), Postives = 158/243 (65.02%), Query Frame = 0


Query: 12  YFLFFAACFLLSSISKASDSEVDDESAFTYYEGTGRGPRSWWKLNPEWESCGKGKYQSPI 71
           +FL F +  ++S  S     EV+DE+ F Y +   +GP +W +L PEW  CGKG  QSPI
Sbjct: 10  FFLIFISITIVS--SSPDHGEVEDETQFNYEKKGEKGPENWGRLKPEWAMCGKGNMQSPI 69

Query: 72  NIVEEEVGVLPELGKLQRDYTPAPALIKNRGHDMTISWK-QDAG-KITINGTRYSLIQCH 131
           ++ ++ V +   LG L+  Y P+ A IKNRGHD+ + ++  +AG  ITINGT Y L Q H
Sbjct: 70  DLTDKRVLIDHNLGYLRSQYLPSNATIKNRGHDIMMKFEGGNAGLGITINGTEYKLQQIH 129

Query: 132 WHTPSEHSFNGTRHVLELHMVHQSQDNKTAVIGILYKYGRPDPFLSRLFHHIKSI---GK 191
           WH+PSEH+ NG R VLE HMVHQS+D + AV+   YK G+PD FL  L  ++K I    +
Sbjct: 130 WHSPSEHTLNGKRFVLEEHMVHQSKDGRNAVVAFFYKLGKPDYFLLTLERYLKRITDTHE 189

Query: 192 EEKEIGIVNPGDIKFGSRKYYRYIGSLTVPPCSEGVVWTILKKVRTVSRDQVRVLANAIH 250
            ++ + +V+P    F S+ YYR+IGSLT PPCSE V+WTI K++RTV+  Q+ +L   +H
Sbjct: 190 SQEFVEMVHPRTFGFESKHYYRFIGSLTTPPCSENVIWTISKEMRTVTLKQLIMLRVTVH 249

The following BLAST results are available for this feature:

BLAST of Carg21121 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7027647.1	8.3e-150	100.00	Alpha carbonic anhydrase 4, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6595685.1	6.0e-148	99.22	Alpha carbonic anhydrase 4, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022966427.1	1.7e-134	95.08	alpha carbonic anhydrase 4-like [Cucurbita maxima]	[more]
XP_023518783.1	3.7e-113	97.01	LOW QUALITY PROTEIN: alpha carbonic anhydrase 4-like [Cucurbita pepo subsp. pepo...	[more]
XP_022925105.1	3.7e-113	97.01	LOW QUALITY PROTEIN: alpha carbonic anhydrase 4-like [Cucurbita moschata]	[more]

BLAST of Carg21121 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F4JIK2	1.6e-74	52.77	Alpha carbonic anhydrase 4 OS=Arabidopsis thaliana OX=3702 GN=ACA4 PE=3 SV=1	[more]
Q8L817	1.8e-67	48.55	Alpha carbonic anhydrase 7 OS=Arabidopsis thaliana OX=3702 GN=ACA7 PE=2 SV=1	[more]
Q9SUB4	1.9e-64	46.31	Alpha carbonic anhydrase 6 OS=Arabidopsis thaliana OX=3702 GN=ACA6 PE=3 SV=1	[more]
F4IHR4	5.8e-61	50.00	Alpha carbonic anhydrase 2 OS=Arabidopsis thaliana OX=3702 GN=ACA2 PE=2 SV=2	[more]
Q84UV8	2.4e-59	47.90	Bifunctional monodehydroascorbate reductase and carbonic anhydrase nectarin-3 OS...	[more]

BLAST of Carg21121 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HMY4	8.2e-135	95.08	alpha carbonic anhydrase 4-like OS=Cucurbita maxima OX=3661 GN=LOC111466081 PE=3...	[more]
A0A6J1EGY9	1.8e-113	97.01	LOW QUALITY PROTEIN: alpha carbonic anhydrase 4-like OS=Cucurbita moschata OX=36...	[more]
A0A7N2M2N8	1.1e-94	65.59	Alpha-carbonic anhydrase domain-containing protein OS=Quercus lobata OX=97700 PE...	[more]
A0A7N2M0S3	1.4e-94	65.59	Alpha-carbonic anhydrase domain-containing protein OS=Quercus lobata OX=97700 PE...	[more]
V4RJW3	1.8e-94	66.80	Alpha-carbonic anhydrase domain-containing protein OS=Citrus clementina OX=85681...	[more]

BLAST of Carg21121 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G20990.1	1.1e-75	52.77	alpha carbonic anhydrase 4	[more]
AT1G08080.1	1.3e-68	48.55	alpha carbonic anhydrase 7	[more]
AT4G21000.1	1.4e-65	46.31	alpha carbonic anhydrase 6	[more]
AT2G28210.1	3.5e-61	50.24	alpha carbonic anhydrase 2	[more]
AT1G08065.1	1.6e-58	46.50	alpha carbonic anhydrase 5	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001148	Alpha carbonic anhydrase domain	SMART	SM01057	Carb_anhydrase_2a	coord: 39..254 e-value: 3.3E-61 score: 219.3
IPR001148	Alpha carbonic anhydrase domain	PFAM	PF00194	Carb_anhydrase	coord: 42..245 e-value: 1.5E-38 score: 132.8
IPR001148	Alpha carbonic anhydrase domain	PROSITE	PS51144	ALPHA_CA_2	coord: 37..258 score: 40.483196
IPR036398	Alpha carbonic anhydrase domain superfamily	GENE3D	3.10.200.10	Alpha carbonic anhydrase	coord: 35..251 e-value: 8.5E-67 score: 227.1
IPR036398	Alpha carbonic anhydrase domain superfamily	SUPERFAMILY	51069	Carbonic anhydrase	coord: 39..245
IPR023561	Carbonic anhydrase, alpha-class	PANTHER	PTHR18952	CARBONIC ANHYDRASE	coord: 14..247
None	No IPR available	PANTHER	PTHR18952:SF246	ALPHA CARBONIC ANHYDRASE 4-RELATED	coord: 14..247
IPR041891	Carbonic anhydrase, prokaryotic-like	CDD	cd03124	alpha_CA_prokaryotic_like	coord: 48..246 e-value: 1.23427E-92 score: 270.297

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg21121-RA	Carg21121-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0009570	chloroplast stroma
molecular_function	GO:0004089	carbonate dehydratase activity
molecular_function	GO:0008270	zinc ion binding