Carg21110 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg21110
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionL-galactono-1,4-lactone dehydrogenase
LocationCarg_Chr07: 8109447 .. 8113982 (+)
RNA-Seq ExpressionCarg21110
SyntenyCarg21110
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATACCTGTTGTCTCTTTCTCCCCCAAGTCTCCATATCCTCCAACTCCAATTTTGGCTACCAACTGGTCAATTCAATTCGTTTACAGACCTTTTTCTGTATAAGCCCGGGAAGAATCGATCTTAACCCACTCCACCAGACACCATCAAATGCTCAGGCTTCTTTCTCTCCGGCGATCCCTTCATCTCCACCACCGCCGTCCCCAATTTCCACCCACCGTCGCCATTTCCAGACCCCCTTTAAATCCCACTCGCCAATTTTCCACTGTTTCTCCTCCACCGCCGTCGTCCTCTTCCGAGTCCGAGCTCCGCAAGTACATTGGCTATGGCGCCCTAGTCATTTTATGCGGCGCCGCCACTTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATATGCGCCTCTCCCCGAGGATCTTCATACTGTGTCCAATTGGAGTGGAACTCACGAGGTTCTTACGAGGGTGTTTCACCAGCCGGAGAATTTGGAGCAGCTTGAGGAAGTGGTGAAGGAGTCTAATGTGAAGAAGGCTAGGATTCGGCCAGTTGGGTCGGGGCTTTCGCCTAATGGGATTGGACTGTCGAGGATGGGGATGGTGAACTTGGCGTTGATGGATGAGGTCTTGGAAGTGGATAAGGAGAAGAAGAGAGTTAGGGTTCAGGCGGGGATAAGGGTTCAGCAGCTTGTCGATGGAATCAAGGAGTATGGGCTTACTTTGCAAAATTTTGCTTCTATCAGGGAGCAGCAGATCGGTGGCATCATTCAGGTTATTTTATGAGAATTTCTGTGGTTTTGATGTTGTTCAATTGCTAATTGAAGTACTATGTTGGATTGTGTGCTGCATTTGATCACTTTGAACACTTGAACTTCAATGCCATGGCTGTATGTCGGATAAATATGAGTAGTTGTCTGTCCAGGGCTGTAAATTAGTTTGTTTGCAGTGAAGAAATTTGATCTTGCTAGCTTCCCATCTATTTTGATTGGATTGTATTTTTTATTTTGTATTTTGTATTTCTCCTTGTGATAGAATTGTCATGGATATGATTTTAATGGCTGTCCGAGCTTAGAAGTAGTGCTAGTACCATTCAAGAGGTGAATTTTGAGACAAAACATATCTCGTTCGAAAGAGAAACGATCAAATTGTTGCCACACATAGCGTATCAAGTGAACATTTATCGATTAGACTAGTTATTTTTCTTTAGGTATGTTGCTTAATTGGATTCCCTTTTTATTTTAGTTTGACTCCTTTTGGTTGGGGCTTTGTTTTTGTATTCACATTGTACGGGGTAATAATCCACCTTTTGTGGGATTATTCCCGGGAGGAGAACGGAAATAGGCTGCGAATGCTGGTCGTGGTAGGTTCGAGGATCTTTTATTTGTCCTCAACAAAAGTTAGGATATTCATAAAAAAGATTTGATTATTTAGGTGCCAATAACATGTGGGATGAGATGAAAGTTTGGTGGTTTTAATTCTTTATGAAAAGTCTTTTTTGGTCTCCTTTGATTTTGTCACTAAAAGAACATATCATCCTGTCTTTTGGTTTCTTTAATGAAATGTTTCCAATCCTCCTCTCCACAAGGTGGAGTTCCTGATATTGCATGTAATATGACATTTTGATTGTCATGTTTTTTCTTGAGCTGAGCCGATTATGCTTCTAGGTCGGTGCACATGGTACTGGTGCAAAATTGCCTCCCATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCAGCCAAGGGTACTATAGAGATTTCCAGAGAGAAAGATCCCGAGCTTTTTTATCTAGCTCGCTGTGGTCTTGGCGGCCTTGGAGTCGTCGCTGAAGTAACCCTCCAGTGCGTTGAGAGACAGGAACTTGTGGAACACACGTACATTTCGAACATGAAAGACATCAAGAAGAACCACAAGTAATTATGTACTTTTTGATTGTTTATTTTATGCCATTCTATAGTATGTTGCATACTACAGTTAGAAATGATAGTGTATTGTGATATAATTTGATTTATTTTGCCAGTGAACCGTCTAAGTTCTGAAGTTCTCTATCTTTGCAGGAAATTGTTGGCTGACAATAAGCATGTGAAATACCTGTATATCCCTTACACTGATGCCATTGTTGTTGTGACTTGCAATCCTATCTCAAAATGGAGAGGTCCTCCCAAATTTCAACCGAAATATACCAGTGATGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAATCTCTTCAAAAATACAGGTTAGTGCCGTCTTCTGTTTGAGTATTTCGTTATCTATAATTTCGAAAGGAGGTGGACACGAGGCGGTGTGCTAGGAAGGACGTTGAGTCTCGAAGGGGGTGGATTGGGGGTGGGGGTGGGGGGGGGGTTGGGTTCCACATTGAATGGAAAAGGGAACGAGTGCCAGCGAGGACGCTCGGCCCCGAAGGGGGTCGATTGTGAGATCCCACGTCAGTTGGGGAGGAGAATGAAACATTATTTATAAGGGTGTGAAAACCTGTTTCTAGCATACGCGTCTTAAAAACCTTGAGGTTGCCCGAAAGGGAAAGTCCAGAGAAGACAATATCTAGCGGTGGGCTTGAGATGTTACGTTTATTCTCTTAAATCACTTTCTATTCCTTTGTTTTGGTTGATATTCATTTTTTTTTATCATGAACTTAAAATTTTGTTCGAAGTAGTATTCATTTCATCTGATTGCTTGCTTGATAATTGCAGTGCTAGTGATGAAAGAGATATAAATGAGTTTTCATTTACTGAGTTGCGTGACAAACTACTTGCCCTTGATCCTCTGAACAAAGAGCATGTTGTGAAAGTCAATCAAGCAGAGGCTGAATTCTGGAGGAAGTCGGAAGGATACCGAGTCGGATGGAGCGACGAAATTTTAGGTTTTGATTGTGGTGGCCAACAATGGGTCTCCGAGACTTGTTTTCCTGCTGGTACTCTTGCAAAACCTAACATGAAGGACATTGAATACATAGAGGAATTAAAGCAGCTTATTGAAAAAGAAAACATACCTGCACCTGCTCCCATTGAGCAGCGCTGGACTGCTTGTAGCAAGAGCCCCATGAGTCCAGCTTCAAGCTCAGCTGAGGATGATATATTCTCTTGGGTAAACTCTAAACATCAATCTTTGTATTTTCTCTCTTTCAAGTTTTGAATCTATACTCGTGCAGCAAGAAAGCGGAATGTTTAGAGATACCCTGCAAGATCTTAGAACTTAATAACTTATTGTTCATCGTCGTTTTTTAGAGTAAGATTATGATTTAGATATATGCAAAGTCTTCTTGTAGATTAGATTAGAGGGAATGTCCTCGAGTACGTGGGCTATTCGGCATAAAGGGATAAGCATGAGATATTTTTCAAGACATAGCCCACCGCTAGCAGATATTGTGTTCTTTTGGCTTTACCTTTCGAGCTCCCTTTTAAAATTTTTAAAACGCGTCTACTAGGAGGTTTCCACACCCTAGTAGACGAGTTTTAAAAAACTTTGAAGGGAAGGCCGAAAGGAAAAACTCAAAGAGGATAATATCTGCACGGGCTGTTACACGTAGTGAAAGTAAATGAAGTGTCATAAAGTTACTTCTAAACGGGTCAACTCGAACCAGTTGCTTCTAAACGGGTCAACTGGACTGTGGTTCGTTGTTTGGTTATGTTGTGCATTTAATCAAGGATCATTTGATGCGAACAGGTCGGAATAATAATGTACCTGCCTACTTCAGACGCACGCCAGAGAAAGGAAATAACAGAGGAGTTCTTTCACTACAGACACCTCACTCAAACACTGCTGTGGGATCAATATTCCGCCTTCGAACATTGGGCTAAGATTGAGGTACCCAGTTTCAAAATCTTGTTTCTAAACTACCTCGAAGTATAACGAACGATTAACCGAGCAAAATGGCGTGAAAAGTTGACGTGTTTGAACGAATGTACTCTCAGGTTCCAAGGGACAAGGATGAGCTTGCAGCCCTTCAAGCAAGACTTAGGAAACGGTTTCCTGTCGATGAATACAACAAGGCACGACGGGAATTGGACCCGAACAAAATTCTTTCGAGTAACAAGTTAGAAAAGATGTTCGTGTCATCGGACGTGGTCTGAGATGCACATTTGTTCTTAGTGATCAATAAGTTATGCTATATCCAGTTGGGTAGGTGCTGATTTCTTGTGTTTACTGTACATTTTTCACAGGAATGCTGTATTTCTCATCTCCTGTGGTATATTTTTGCCATAAAAAGTTTACTTGTGGAAGGAATTTCTTCACGTCACGTTGTTCATCACAGTTTCAGTTTCAAATAATTATACCAAAAAACCCCTCTTTTTTCGGTTTTTATTTTTGCTCTTTGAATCTGGAATGTGGGCTGTGATGGAAGAAACCCTTTGTTTTGTGCGTGAAATCGTAGGGATTTGAGTGGTTTGAATGATTAGAAGTATCGGCGTTCTTCTTTCTTTCTTCCGTGTTTATTGAGATTTTGGGATGAATTTACGTTTTGAGTTATGGGAATGGTTGAGAATATAATAT

mRNA sequence

ATACCTGTTGTCTCTTTCTCCCCCAAGTCTCCATATCCTCCAACTCCAATTTTGGCTACCAACTGGTCAATTCAATTCGTTTACAGACCTTTTTCTGTATAAGCCCGGGAAGAATCGATCTTAACCCACTCCACCAGACACCATCAAATGCTCAGGCTTCTTTCTCTCCGGCGATCCCTTCATCTCCACCACCGCCGTCCCCAATTTCCACCCACCGTCGCCATTTCCAGACCCCCTTTAAATCCCACTCGCCAATTTTCCACTGTTTCTCCTCCACCGCCGTCGTCCTCTTCCGAGTCCGAGCTCCGCAAGTACATTGGCTATGGCGCCCTAGTCATTTTATGCGGCGCCGCCACTTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATATGCGCCTCTCCCCGAGGATCTTCATACTGTGTCCAATTGGAGTGGAACTCACGAGGTTCTTACGAGGGTGTTTCACCAGCCGGAGAATTTGGAGCAGCTTGAGGAAGTGGTGAAGGAGTCTAATGTGAAGAAGGCTAGGATTCGGCCAGTTGGGTCGGGGCTTTCGCCTAATGGGATTGGACTGTCGAGGATGGGGATGGTGAACTTGGCGTTGATGGATGAGGTCTTGGAAGTGGATAAGGAGAAGAAGAGAGTTAGGGTTCAGGCGGGGATAAGGGTTCAGCAGCTTGTCGATGGAATCAAGGAGTATGGGCTTACTTTGCAAAATTTTGCTTCTATCAGGGAGCAGCAGATCGGTGGCATCATTCAGGTCGGTGCACATGGTACTGGTGCAAAATTGCCTCCCATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCAGCCAAGGGTACTATAGAGATTTCCAGAGAGAAAGATCCCGAGCTTTTTTATCTAGCTCGCTGTGGTCTTGGCGGCCTTGGAGTCGTCGCTGAAGTAACCCTCCAGTGCGTTGAGAGACAGGAACTTGTGGAACACACGTACATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTGTTGGCTGACAATAAGCATGTGAAATACCTGTATATCCCTTACACTGATGCCATTGTTGTTGTGACTTGCAATCCTATCTCAAAATGGAGAGGTCCTCCCAAATTTCAACCGAAATATACCAGTGATGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAATCTCTTCAAAAATACAGTGCTAGTGATGAAAGAGATATAAATGAGTTTTCATTTACTGAGTTGCGTGACAAACTACTTGCCCTTGATCCTCTGAACAAAGAGCATGTTGTGAAAGTCAATCAAGCAGAGGCTGAATTCTGGAGGAAGTCGGAAGGATACCGAGTCGGATGGAGCGACGAAATTTTAGGTTTTGATTGTGGTGGCCAACAATGGGTCTCCGAGACTTGTTTTCCTGCTGGTACTCTTGCAAAACCTAACATGAAGGACATTGAATACATAGAGGAATTAAAGCAGCTTATTGAAAAAGAAAACATACCTGCACCTGCTCCCATTGAGCAGCGCTGGACTGCTTGTAGCAAGAGCCCCATGAGTCCAGCTTCAAGCTCAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATAATGTACCTGCCTACTTCAGACGCACGCCAGAGAAAGGAAATAACAGAGGAGTTCTTTCACTACAGACACCTCACTCAAACACTGCTGTGGGATCAATATTCCGCCTTCGAACATTGGGCTAAGATTGAGGTTCCAAGGGACAAGGATGAGCTTGCAGCCCTTCAAGCAAGACTTAGGAAACGGTTTCCTGTCGATGAATACAACAAGGCACGACGGGAATTGGACCCGAACAAAATTCTTTCGAGTAACAAGTTAGAAAAGATGTTCGTGTCATCGGACGTGGTCTGAGATGCACATTTGTTCTTAGTGATCAATAAGTTATGCTATATCCAGTTGGGTAGGTGCTGATTTCTTGTGTTTACTGTACATTTTTCACAGGAATGCTGTATTTCTCATCTCCTGTGGTATATTTTTGCCATAAAAAGTTTACTTGTGGAAGGAATTTCTTCACGTCACGTTGTTCATCACAGTTTCAGTTTCAAATAATTATACCAAAAAACCCCTCTTTTTTCGGTTTTTATTTTTGCTCTTTGAATCTGGAATGTGGGCTGTGATGGAAGAAACCCTTTGTTTTGTGCGTGAAATCGTAGGGATTTGAGTGGTTTGAATGATTAGAAGTATCGGCGTTCTTCTTTCTTTCTTCCGTGTTTATTGAGATTTTGGGATGAATTTACGTTTTGAGTTATGGGAATGGTTGAGAATATAATAT

Coding sequence (CDS)

ATGCTCAGGCTTCTTTCTCTCCGGCGATCCCTTCATCTCCACCACCGCCGTCCCCAATTTCCACCCACCGTCGCCATTTCCAGACCCCCTTTAAATCCCACTCGCCAATTTTCCACTGTTTCTCCTCCACCGCCGTCGTCCTCTTCCGAGTCCGAGCTCCGCAAGTACATTGGCTATGGCGCCCTAGTCATTTTATGCGGCGCCGCCACTTACTACTCCTTCCCTTTCCCCGAAAACGCCAAGCACAAGAAGGCCCAGATCTTCCGATATGCGCCTCTCCCCGAGGATCTTCATACTGTGTCCAATTGGAGTGGAACTCACGAGGTTCTTACGAGGGTGTTTCACCAGCCGGAGAATTTGGAGCAGCTTGAGGAAGTGGTGAAGGAGTCTAATGTGAAGAAGGCTAGGATTCGGCCAGTTGGGTCGGGGCTTTCGCCTAATGGGATTGGACTGTCGAGGATGGGGATGGTGAACTTGGCGTTGATGGATGAGGTCTTGGAAGTGGATAAGGAGAAGAAGAGAGTTAGGGTTCAGGCGGGGATAAGGGTTCAGCAGCTTGTCGATGGAATCAAGGAGTATGGGCTTACTTTGCAAAATTTTGCTTCTATCAGGGAGCAGCAGATCGGTGGCATCATTCAGGTCGGTGCACATGGTACTGGTGCAAAATTGCCTCCCATTGATGAGCAGGTTATTGCTATGAAATTGGTCACGCCAGCCAAGGGTACTATAGAGATTTCCAGAGAGAAAGATCCCGAGCTTTTTTATCTAGCTCGCTGTGGTCTTGGCGGCCTTGGAGTCGTCGCTGAAGTAACCCTCCAGTGCGTTGAGAGACAGGAACTTGTGGAACACACGTACATTTCGAACATGAAAGACATCAAGAAGAACCACAAGAAATTGTTGGCTGACAATAAGCATGTGAAATACCTGTATATCCCTTACACTGATGCCATTGTTGTTGTGACTTGCAATCCTATCTCAAAATGGAGAGGTCCTCCCAAATTTCAACCGAAATATACCAGTGATGAAGCCATACAGCATGTTCGTGATCTCTACGTGGAATCTCTTCAAAAATACAGTGCTAGTGATGAAAGAGATATAAATGAGTTTTCATTTACTGAGTTGCGTGACAAACTACTTGCCCTTGATCCTCTGAACAAAGAGCATGTTGTGAAAGTCAATCAAGCAGAGGCTGAATTCTGGAGGAAGTCGGAAGGATACCGAGTCGGATGGAGCGACGAAATTTTAGGTTTTGATTGTGGTGGCCAACAATGGGTCTCCGAGACTTGTTTTCCTGCTGGTACTCTTGCAAAACCTAACATGAAGGACATTGAATACATAGAGGAATTAAAGCAGCTTATTGAAAAAGAAAACATACCTGCACCTGCTCCCATTGAGCAGCGCTGGACTGCTTGTAGCAAGAGCCCCATGAGTCCAGCTTCAAGCTCAGCTGAGGATGATATATTCTCTTGGGTCGGAATAATAATGTACCTGCCTACTTCAGACGCACGCCAGAGAAAGGAAATAACAGAGGAGTTCTTTCACTACAGACACCTCACTCAAACACTGCTGTGGGATCAATATTCCGCCTTCGAACATTGGGCTAAGATTGAGGTTCCAAGGGACAAGGATGAGCTTGCAGCCCTTCAAGCAAGACTTAGGAAACGGTTTCCTGTCGATGAATACAACAAGGCACGACGGGAATTGGACCCGAACAAAATTCTTTCGAGTAACAAGTTAGAAAAGATGTTCGTGTCATCGGACGTGGTCTGA

Protein sequence

MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPRDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV
Homology
BLAST of Carg21110 vs. NCBI nr
Match: KAG7027658.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1183.3 bits (3060), Expect = 0.0e+00
Identity = 589/589 (100.00%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG
Sbjct: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL
Sbjct: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR
Sbjct: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

BLAST of Carg21110 vs. NCBI nr
Match: KAG6595695.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1180.6 bits (3053), Expect = 0.0e+00
Identity = 587/589 (99.66%), Postives = 589/589 (100.00%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG
Sbjct: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL
Sbjct: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESL+KYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR
Sbjct: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKL+KMFVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLDKMFVSSDVV 589

BLAST of Carg21110 vs. NCBI nr
Match: XP_022925153.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 586/589 (99.49%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           MLRLLSLRRSLHLHHRRP FPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG
Sbjct: 1   MLRLLSLRRSLHLHHRRPHFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL
Sbjct: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESL+KYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP+
Sbjct: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

BLAST of Carg21110 vs. NCBI nr
Match: XP_023517263.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1169.5 bits (3024), Expect = 0.0e+00
Identity = 579/589 (98.30%), Postives = 587/589 (99.66%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           ML+LLSLRRSLHLHHRRPQFPPTVAIS+PPLNPTRQFSTVSPPPPSSSSESELRKY+GYG
Sbjct: 1   MLKLLSLRRSLHLHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL
Sbjct: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMD VLEVDKEKKRVRVQAG
Sbjct: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDRVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEIS++KDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISKDKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESL+KYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP+
Sbjct: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKAR ELDPNKILSSNKLEK+FVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARGELDPNKILSSNKLEKLFVSSDVV 589

BLAST of Carg21110 vs. NCBI nr
Match: XP_022966248.1 (L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 572/589 (97.11%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           ML+LLSLRRSLHLHHRRPQFPPTVAIS+PPLNPTRQFSTVSPPPPSSSSESELRKY+GYG
Sbjct: 1   MLKLLSLRRSLHLHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVI CGA TYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQP+NL
Sbjct: 61  ALVIFCGATTYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVHTRVFHQPDNL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQL+EVVKE+NVKKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRVQAG
Sbjct: 121 EQLQEVVKEANVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEIS+EKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESL+KYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFW KSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWWKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SS+EDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP+
Sbjct: 481 SSSEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKAR+ELDPNKILSSNKLEK+FVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARQELDPNKILSSNKLEKLFVSSDVV 589

BLAST of Carg21110 vs. ExPASy Swiss-Prot
Match: Q9SU56 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=GLDH PE=1 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 2.9e-270
Identity = 465/606 (76.73%), Postives = 516/606 (85.15%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVS--------------PPPPS 60
           MLR L LRRS  + H      P+ +  R   +P R   T                PPPP+
Sbjct: 1   MLRSLLLRRS--VGHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPPA 60

Query: 61  SSSESELRKYIGYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT 120
           ++SE++ RKY GY AL I  G ATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT
Sbjct: 61  TASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT 120

Query: 121 HEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVL 180
           HEV TR F+QPENL  LE +VKES+ KK RIRPVGSGLSPNGIGLSR GMVNLALMD+VL
Sbjct: 121 HEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKVL 180

Query: 181 EVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPI 240
           EVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPPI
Sbjct: 181 EVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPI 240

Query: 241 DEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYI 300
           DEQVI+MKLVTPAKGTIE+SREKDPELF+LARCGLGGLGVVAEVTLQCV R ELVEHTY+
Sbjct: 241 DEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTYV 300

Query: 301 SNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQH 360
           SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK +PKYT+DEA+QH
Sbjct: 301 SNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQH 360

Query: 361 VRDLYVESLQKYSASD---------ERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEA 420
           VRDLY ES+ KY   D         E DI E SFTELRDKLLALDPLN  HV KVNQAEA
Sbjct: 361 VRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAEA 420

Query: 421 EFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKEN 480
           EFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEKE 
Sbjct: 421 EFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKEA 480

Query: 481 IPAPAPIEQRWTACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRH 540
           IPAPAPIEQRWTA SKSP+SPA S++EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHYRH
Sbjct: 481 IPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHYRH 540

Query: 541 LTQTLLWDQYSAFEHWAKIEVPRDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSN 584
           LTQ  LWDQ+SA+EHWAKIE+P+DK+EL ALQAR+RKRFPVD YNKARRELDPN+ILS+N
Sbjct: 541 LTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILSNN 600

BLAST of Carg21110 vs. ExPASy Swiss-Prot
Match: O47881 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=3712 PE=1 SV=1)

HSP 1 Score: 927.2 bits (2395), Expect = 9.5e-269
Identity = 464/595 (77.98%), Postives = 514/595 (86.39%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPP--TVAISRPPLNPTRQFSTVSPPPPSSS-SESELRKYI 60
           MLR L LRRS +    RP FPP  T+  S   L P        PPP SSS SE E RKY 
Sbjct: 1   MLRSLLLRRS-NARSLRPPFPPLRTLCTSGQTLTPAPPPPPPPPPPISSSASEKEFRKYA 60

Query: 61  GYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQP 120
           GY AL +  GAATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TR F+QP
Sbjct: 61  GYAALALFSGAATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRNFNQP 120

Query: 121 ENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRV 180
           E L  LE +VKE++ KK RIRPVGSGLSPNGIGLSR GMVNLALMD+VLEVDKEKKRVRV
Sbjct: 121 ETLADLEALVKEAHEKKNRIRPVGSGLSPNGIGLSRSGMVNLALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVD I+EYGLTLQNFASIREQQIGGIIQVGAHGTGA+LPPIDEQVI MKLVT
Sbjct: 181 QAGIRVQQLVDAIQEYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPIDEQVIGMKLVT 240

Query: 241 PAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIE+S++ DPELF+LARCGLGGLGVVAEVTLQCVERQEL+EHTY+S +++IKKNHK
Sbjct: 241 PAKGTIELSKDNDPELFHLARCGLGGLGVVAEVTLQCVERQELLEHTYVSTLEEIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQK 360
           KLL+ NKHVKYLYIPYTD +VVVTCNP+SKW G PK +PKYT++EA++HVRDLY ES+ K
Sbjct: 301 KLLSTNKHVKYLYIPYTDTVVVVTCNPVSKWSGAPKDKPKYTTEEALKHVRDLYRESIVK 360

Query: 361 YSASD---------ERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRV 420
           Y   D         E DINE SFTELRDKL+ALDPLN  HV KVNQAEAEFW+KSEGYRV
Sbjct: 361 YRVQDSSKKTPDSREPDINELSFTELRDKLIALDPLNDVHVGKVNQAEAEFWKKSEGYRV 420

Query: 421 GWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRW 480
           GWSDEILGFDCGGQQWVSETCFPAGTLAKP+MKD+EYIE+LK+LI+KE IPAP+PIEQRW
Sbjct: 421 GWSDEILGFDCGGQQWVSETCFPAGTLAKPSMKDLEYIEQLKELIQKEAIPAPSPIEQRW 480

Query: 481 TACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYS 540
           T  SKSPMSPA S+AE+DIFSWVGIIMYLPT+D RQRK+IT+EFFHYRHLTQ  LWDQYS
Sbjct: 481 TGRSKSPMSPAFSTAEEDIFSWVGIIMYLPTADPRQRKDITDEFFHYRHLTQAKLWDQYS 540

Query: 541 AFEHWAKIEVPRDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMF 584
           A+EHWAKIE+P+DK+EL ALQ RLRKRFPVD YNKARRELDPN+ILS+N +EK+F
Sbjct: 541 AYEHWAKIEIPKDKEELEALQERLRKRFPVDAYNKARRELDPNRILSNNMVEKLF 594

BLAST of Carg21110 vs. ExPASy Swiss-Prot
Match: Q2RAP0 (L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH1 PE=2 SV=1)

HSP 1 Score: 850.5 bits (2196), Expect = 1.1e-245
Identity = 413/561 (73.62%), Postives = 487/561 (86.81%), Query Frame = 0

Query: 31  LNPTRQFSTVSPPPPSSSSESELRKYIGYGALVILCGAATYYSFPFPENAKHKKAQIFRY 90
           L+  R  S  SP P   +S+++LRKY GY  L++ CGAATYYSFP P +A HKKA  F+Y
Sbjct: 23  LHLVRALSASSPLP---ASDADLRKYAGYALLLLGCGAATYYSFPLPPDALHKKAVPFKY 82

Query: 91  APLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIG 150
           APLP+DLH VSNWS THEV TRV  QP++L  L + +  ++ +  ++RP+GSGLSPNG+ 
Sbjct: 83  APLPDDLHAVSNWSATHEVHTRVLLQPDSLPALHDALAAAHGECRKLRPLGSGLSPNGLA 142

Query: 151 LSRMGMVNLALMDEVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGG 210
           LSR GMVNLALMD+VL VD +KK V VQAGIRV +LVD ++E+GLTLQNFASIREQQ+GG
Sbjct: 143 LSRAGMVNLALMDKVLGVDAKKKTVTVQAGIRVAELVDALREHGLTLQNFASIREQQVGG 202

Query: 211 IIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEV 270
           IIQVGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+SREKDP+LFYLARCGLGGLGVVAEV
Sbjct: 203 IIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYLARCGLGGLGVVAEV 262

Query: 271 TLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRG 330
           TLQCVER +L+EHT++SN  ++KKNHKK L++NKH+KYL+IPYTD +VVV CNP S+WR 
Sbjct: 263 TLQCVERHQLIEHTFVSNADEVKKNHKKWLSENKHIKYLWIPYTDTVVVVQCNPPSRWR- 322

Query: 331 PPKFQPKYTSDEAIQHVRDLYVESLQKY---SASDERDINEFSFTELRDKLLALDPLNKE 390
            PKF  KY  DEAIQHVRDLY ESL+KY   + S++ ++++ SFTELRD+LL LDPL+K+
Sbjct: 323 TPKFTSKYGKDEAIQHVRDLYHESLKKYRTKAESNDPEVDQLSFTELRDRLLTLDPLDKD 382

Query: 391 HVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIE 450
           HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKD++YIE
Sbjct: 383 HVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDLDYIE 442

Query: 451 ELKQLIEKENIPAPAPIEQRWTACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKE 510
           EL QLIEKE+IPAPAPIEQRWTACS+SPMSPASSS EDDIFSWVGIIMYLPTSDARQRKE
Sbjct: 443 ELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVGIIMYLPTSDARQRKE 502

Query: 511 ITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPRDKDELAALQARLRKRFPVDEYNKARRE 570
           ITEEFF+YR  TQT LWD YSA+EHWAKIEVP+DKDELA LQARLRKRFPVD YNKAR E
Sbjct: 503 ITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELAELQARLRKRFPVDAYNKARME 562

Query: 571 LDPNKILSSNKLEKMFVSSDV 589
           LDPNK+LS+ KLEK+F  ++V
Sbjct: 563 LDPNKVLSNAKLEKLFPVTEV 579

BLAST of Carg21110 vs. ExPASy Swiss-Prot
Match: Q2QXY1 (L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=GLDH2 PE=2 SV=1)

HSP 1 Score: 848.2 bits (2190), Expect = 5.6e-245
Identity = 411/561 (73.26%), Postives = 488/561 (86.99%), Query Frame = 0

Query: 31  LNPTRQFSTVSPPPPSSSSESELRKYIGYGALVILCGAATYYSFPFPENAKHKKAQIFRY 90
           L+  R  S  SP P   +S+++LRKY GY  L++ CGAATYYSFP P +A HKKA  F+Y
Sbjct: 23  LHLVRALSASSPLP---ASDADLRKYAGYALLLLGCGAATYYSFPLPPDALHKKAVPFKY 82

Query: 91  APLPEDLHTVSNWSGTHEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIG 150
           APLP+DLH VSNWS THEV TRV  QP++L  L + +  ++ ++ ++RP+GSGLSPNG+ 
Sbjct: 83  APLPDDLHAVSNWSATHEVHTRVLLQPDSLPVLHDALAAAHGERRKLRPLGSGLSPNGLA 142

Query: 151 LSRMGMVNLALMDEVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGG 210
           LSR GMVNLALMD+VL+VD +KK V VQAGIRV +LVD ++E+GLTLQNFASIREQQ+GG
Sbjct: 143 LSRAGMVNLALMDKVLDVDAKKKTVTVQAGIRVAELVDTLREHGLTLQNFASIREQQVGG 202

Query: 211 IIQVGAHGTGAKLPPIDEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEV 270
           IIQVGAHGTGA+LPPIDEQVI+MKLVTPAKGTIE+SREKDP+LFYLARCGLGGLGVVAEV
Sbjct: 203 IIQVGAHGTGARLPPIDEQVISMKLVTPAKGTIELSREKDPDLFYLARCGLGGLGVVAEV 262

Query: 271 TLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRG 330
           TLQCVER +L+EHT++S+  ++KKNHKK L++NKH+KYL+IPYTD +VVV CNP S+WR 
Sbjct: 263 TLQCVERHQLIEHTFVSSADEVKKNHKKWLSENKHIKYLWIPYTDTVVVVQCNPPSRWR- 322

Query: 331 PPKFQPKYTSDEAIQHVRDLYVESLQKY---SASDERDINEFSFTELRDKLLALDPLNKE 390
            PKF  KY  DEAIQHVRDLY ESL+KY   + S++ ++++ SFTELRD+LLALDPL+K+
Sbjct: 323 TPKFTSKYGKDEAIQHVRDLYRESLKKYRTKAESNDPEVDQLSFTELRDRLLALDPLDKD 382

Query: 391 HVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIE 450
           HV+++N+AEAE+W+KSEGYR+GWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKD++YIE
Sbjct: 383 HVIRINKAEAEYWKKSEGYRMGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDLDYIE 442

Query: 451 ELKQLIEKENIPAPAPIEQRWTACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKE 510
           EL QLIEKE+IPAPAPIEQRWTACS+SPMSPASSS EDDIFSWVGIIMYLPTSDARQRKE
Sbjct: 443 ELLQLIEKEDIPAPAPIEQRWTACSRSPMSPASSSQEDDIFSWVGIIMYLPTSDARQRKE 502

Query: 511 ITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPRDKDELAALQARLRKRFPVDEYNKARRE 570
           ITEEFF+YR  TQT LWD YSA+EHWAKIEVP+DKDEL  L ARLRKRFPVD YNKAR E
Sbjct: 503 ITEEFFNYRSKTQTNLWDGYSAYEHWAKIEVPKDKDELTELLARLRKRFPVDAYNKARME 562

Query: 571 LDPNKILSSNKLEKMFVSSDV 589
           LDPNK+LS+ KLEK+F  ++V
Sbjct: 563 LDPNKVLSNAKLEKLFPVTEV 579

BLAST of Carg21110 vs. ExPASy Swiss-Prot
Match: P10867 (L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3)

HSP 1 Score: 155.6 bits (392), Expect = 1.7e-36
Identity = 127/484 (26.24%), Postives = 221/484 (45.66%), Query Frame = 0

Query: 102 NWSGTHEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLAL 161
           NW+ T+     V++QP ++E++ EV+  +  +K +++ VG G SP+ I  +   M+++  
Sbjct: 12  NWAKTYGCSPEVYYQPTSVEEVREVLALAREQKKKVKVVGGGHSPSDIACTDGFMIHMGK 71

Query: 162 MDEVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGA 221
           M+ VL+VDKEKK++ V+AGI +  L   + E+GL + N  ++ +  + G+I  G H TG 
Sbjct: 72  MNRVLQVDKEKKQITVEAGILLADLHPQLDEHGLAMSNLGAVSDVTVAGVIGSGTHNTGI 131

Query: 222 KLPPIDEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELV 281
           K   +  QV+A+ L+T     +E S  ++ ++F  AR  LG LG++  VTLQCV +  L 
Sbjct: 132 KHGILATQVVALTLMTADGEVLECSESRNADVFQAARVHLGCLGIILTVTLQCVPQFHLQ 191

Query: 282 EHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSD 341
           E ++ S +K++  N    L  +++ ++L+ P+T+ + ++  +  +K    P     +  D
Sbjct: 192 ETSFPSTLKEVLDNLDSHLKRSEYFRFLWFPHTENVSIIYQDHTNK---APSSASNWFWD 251

Query: 342 EAIQHVRDLYVESLQKYSASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWR 401
            AI      ++     Y       IN F F  L                        F  
Sbjct: 252 YAIGFYLLEFLLWTSTYLPCLVGWINRFFFWML------------------------FNC 311

Query: 402 KSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKE-NIPA 461
           K E   +  S +I  ++C  +Q V +        A P  K  E + ELK ++E    + A
Sbjct: 312 KKESSNL--SHKIFTYECRFKQHVQD-------WAIPREKTKEALLELKAMLEAHPKVVA 371

Query: 462 PAPIEQRWTACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQ 521
             P+E R+T      +SP           ++ IIMY P      R +          L  
Sbjct: 372 HYPVEVRFTRGDDILLSPCFQRDS----CYMNIIMYRPYGKDVPRLDY--------WLAY 431

Query: 522 TLLWDQYSAFEHWAKIEVPRDKDELAALQARLRKRFPV-DEYNKARRELDPNKILSSNKL 581
             +  ++    HWAK      KD          + +P   ++   R +LDP  +  ++ L
Sbjct: 432 ETIMKKFGGRPHWAKAHNCTQKD--------FEEMYPTFHKFCDIREKLDPTGMFLNSYL 439

Query: 582 EKMF 584
           EK+F
Sbjct: 492 EKVF 439

BLAST of Carg21110 vs. ExPASy TrEMBL
Match: A0A6J1EEF4 (L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111432481 PE=4 SV=1)

HSP 1 Score: 1178.7 bits (3048), Expect = 0.0e+00
Identity = 586/589 (99.49%), Postives = 588/589 (99.83%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           MLRLLSLRRSLHLHHRRP FPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG
Sbjct: 1   MLRLLSLRRSLHLHHRRPHFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL
Sbjct: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG
Sbjct: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESL+KYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP+
Sbjct: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 589

BLAST of Carg21110 vs. ExPASy TrEMBL
Match: A0A6J1HRG8 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111465966 PE=4 SV=1)

HSP 1 Score: 1158.7 bits (2996), Expect = 0.0e+00
Identity = 572/589 (97.11%), Postives = 585/589 (99.32%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGYG 60
           ML+LLSLRRSLHLHHRRPQFPPTVAIS+PPLNPTRQFSTVSPPPPSSSSESELRKY+GYG
Sbjct: 1   MLKLLSLRRSLHLHHRRPQFPPTVAISKPPLNPTRQFSTVSPPPPSSSSESELRKYLGYG 60

Query: 61  ALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPENL 120
           ALVI CGA TYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQP+NL
Sbjct: 61  ALVIFCGATTYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVHTRVFHQPDNL 120

Query: 121 EQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQAG 180
           EQL+EVVKE+NVKKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRVQAG
Sbjct: 121 EQLQEVVKEANVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQAG 180

Query: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240
           IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK
Sbjct: 181 IRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPAK 240

Query: 241 GTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300
           GTIEIS+EKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL
Sbjct: 241 GTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKLL 300

Query: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYSA 360
           ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESL+KYSA
Sbjct: 301 ADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLKKYSA 360

Query: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDCG 420
           SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFW KSEGYRVGWSDEILGFDCG
Sbjct: 361 SDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWWKSEGYRVGWSDEILGFDCG 420

Query: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480
           GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS
Sbjct: 421 GQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPAS 480

Query: 481 SSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPR 540
           SS+EDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP+
Sbjct: 481 SSSEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVPK 540

Query: 541 DKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           DKDELAALQARLRKRFPVDEYNKAR+ELDPNKILSSNKLEK+FVSSDVV
Sbjct: 541 DKDELAALQARLRKRFPVDEYNKARQELDPNKILSSNKLEKLFVSSDVV 589

BLAST of Carg21110 vs. ExPASy TrEMBL
Match: E7E016 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 1095.9 bits (2833), Expect = 0.0e+00
Identity = 543/590 (92.03%), Postives = 566/590 (95.93%), Query Frame = 0

Query: 1   MLRLLSLRRSLH-LHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGY 60
           ML  LSLRRS+H   HRR Q  PT AIS+PP NP R FSTVSP P SSS +SELRKY+GY
Sbjct: 1   MLNFLSLRRSIHYFRHRRLQISPTAAISKPPFNPPRPFSTVSPSPSSSSFDSELRKYLGY 60

Query: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVFHQPEN
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVWTRVFHQPEN 120

Query: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQA 180
           LEQLE+VVK++N KKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEIS++KDPELF LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISKDKDPELFLLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESL+KYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS+ERD+NE SFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGYRVGWSDEILGFDC
Sbjct: 361 ASEERDMNEISFTELRDKLLALDPLNKEHVIKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEK+NIPAPAP+EQRWTA SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKKNIPAPAPLEQRWTARSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540

Query: 541 RDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           +DKDELAALQARLRKRFPVDEYNKARR LDPNKILS+NKLEK+F S+D V
Sbjct: 541 KDKDELAALQARLRKRFPVDEYNKARRALDPNKILSNNKLEKLFSSTDTV 590

BLAST of Carg21110 vs. ExPASy TrEMBL
Match: Q9M4U9 (L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE=2 SV=2)

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 538/590 (91.19%), Postives = 565/590 (95.76%), Query Frame = 0

Query: 1   MLRLLSLRRSLH-LHHRRPQFPPTVAISRPPLNPTRQFSTVSPPPPSSSSESELRKYIGY 60
           ML  LSLRRSLH   HRR Q  PT AIS+PPLN  R F TVS P PSSS +SELRKY+GY
Sbjct: 1   MLNFLSLRRSLHYFRHRRLQISPTAAISKPPLNSPRPFCTVS-PSPSSSFDSELRKYLGY 60

Query: 61  GALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPEN 120
           GALVI CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPE+
Sbjct: 61  GALVIFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQPED 120

Query: 121 LEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRVQA 180
           LEQLE+VVK++N KKAR+RPVGSGLSPNGIGLSR+GMVN+ALMD+VLEVDKEKKRVRVQA
Sbjct: 121 LEQLEQVVKQANEKKARVRPVGSGLSPNGIGLSRLGMVNMALMDKVLEVDKEKKRVRVQA 180

Query: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240
           GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA
Sbjct: 181 GIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVTPA 240

Query: 241 KGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300
           KGTIEIS +KDPELF+LARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL
Sbjct: 241 KGTIEISNDKDPELFFLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHKKL 300

Query: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKYS 360
           LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKF+PKYTS+EAIQHVRDLYVESL+KYS
Sbjct: 301 LADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFKPKYTSEEAIQHVRDLYVESLKKYS 360

Query: 361 ASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGFDC 420
           AS+ERDINEFSFTELRDKLLALDPLNKEHV+KVNQAEAEFWRKSEGY+VGWSDEILGFDC
Sbjct: 361 ASEERDINEFSFTELRDKLLALDPLNKEHVMKVNQAEAEFWRKSEGYKVGWSDEILGFDC 420

Query: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMSPA 480
           GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAP+EQRWT  SKSPMSPA
Sbjct: 421 GGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPLEQRWTGRSKSPMSPA 480

Query: 481 SSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIEVP 540
           SS+AEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWD++SAFEHWAKIEVP
Sbjct: 481 SSTAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDKFSAFEHWAKIEVP 540

Query: 541 RDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           +DKDELAALQARLRKRFPVDE NKARR LDPNKILS+NKLEK+F S D+V
Sbjct: 541 KDKDELAALQARLRKRFPVDESNKARRALDPNKILSNNKLEKLFSSLDMV 589

BLAST of Carg21110 vs. ExPASy TrEMBL
Match: A0A6J1DK81 (L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3673 GN=LOC111021307 PE=4 SV=1)

HSP 1 Score: 1062.8 bits (2747), Expect = 5.3e-307
Identity = 528/592 (89.19%), Postives = 555/592 (93.75%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTR---QFSTVSPPPPSSSSESELRKYI 60
           MLR+L LRRS    HRR   PP  AISR PLN       FSTVSPPPPSSS +SELRKY+
Sbjct: 1   MLRILPLRRS----HRRLLSPPAAAISRTPLNHHHHPPSFSTVSPPPPSSSFDSELRKYL 60

Query: 61  GYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVLTRVFHQP 120
           GYGALV+ CGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEV TRVF QP
Sbjct: 61  GYGALVVFCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGTHEVQTRVFQQP 120

Query: 121 ENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVLEVDKEKKRVRV 180
           ENLEQLE+VVKE+N KKARIRPVGSGLSPNGIGLSRMGMVNLALMD+VLEVDKEKKRVRV
Sbjct: 121 ENLEQLEQVVKEANEKKARIRPVGSGLSPNGIGLSRMGMVNLALMDKVLEVDKEKKRVRV 180

Query: 181 QAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPIDEQVIAMKLVT 240
           QAGIRVQQLVDGIKE+GLTLQNFASIREQQIGGIIQVGAHGTG KLPPIDEQVIAMKLVT
Sbjct: 181 QAGIRVQQLVDGIKEHGLTLQNFASIREQQIGGIIQVGAHGTGPKLPPIDEQVIAMKLVT 240

Query: 241 PAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMKDIKKNHK 300
           PAKGTIEIS+EKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNM DIKKNHK
Sbjct: 241 PAKGTIEISKEKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYISNMNDIKKNHK 300

Query: 301 KLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQK 360
           KLL DNKHVKYLYIPYTDA+VVVTCNPISKWRGPPK +PKYTSDEAIQHVRDLY ESL+K
Sbjct: 301 KLLTDNKHVKYLYIPYTDAVVVVTCNPISKWRGPPKLKPKYTSDEAIQHVRDLYAESLKK 360

Query: 361 YSASDERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEAEFWRKSEGYRVGWSDEILGF 420
           YS SDE DINE+S+TELRDKLLALDPLNKEHV KVNQAEAEFWRKSEGY+VGWSDEILGF
Sbjct: 361 YSTSDETDINEYSYTELRDKLLALDPLNKEHVTKVNQAEAEFWRKSEGYKVGWSDEILGF 420

Query: 421 DCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKENIPAPAPIEQRWTACSKSPMS 480
           DCGGQQWVSETCFPAGTLAKP+MKDI+YIE+LKQLIEKENIPAPAPIEQRWTACSKSPMS
Sbjct: 421 DCGGQQWVSETCFPAGTLAKPSMKDIKYIEDLKQLIEKENIPAPAPIEQRWTACSKSPMS 480

Query: 481 PASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRHLTQTLLWDQYSAFEHWAKIE 540
           PASS A+DD+FSWVGIIMYLPT DARQRKEITEEFFHYRHLTQ+ LWDQYSAFEHWAKIE
Sbjct: 481 PASSLAQDDLFSWVGIIMYLPTLDARQRKEITEEFFHYRHLTQSQLWDQYSAFEHWAKIE 540

Query: 541 VPRDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSNKLEKMFVSSDVV 590
           VPRDK+ELAALQARLRKRFPVDE+NKA+R LDPNKILS+NKLEK+F S+D V
Sbjct: 541 VPRDKEELAALQARLRKRFPVDEFNKAQRALDPNKILSNNKLEKLFPSNDAV 588

BLAST of Carg21110 vs. TAIR 10
Match: AT3G47930.1 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 932.2 bits (2408), Expect = 2.1e-271
Identity = 465/606 (76.73%), Postives = 516/606 (85.15%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVS--------------PPPPS 60
           MLR L LRRS  + H      P+ +  R   +P R   T                PPPP+
Sbjct: 1   MLRSLLLRRS--VGHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPPA 60

Query: 61  SSSESELRKYIGYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT 120
           ++SE++ RKY GY AL I  G ATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT
Sbjct: 61  TASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT 120

Query: 121 HEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVL 180
           HEV TR F+QPENL  LE +VKES+ KK RIRPVGSGLSPNGIGLSR GMVNLALMD+VL
Sbjct: 121 HEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKVL 180

Query: 181 EVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPI 240
           EVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPPI
Sbjct: 181 EVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPI 240

Query: 241 DEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYI 300
           DEQVI+MKLVTPAKGTIE+SREKDPELF+LARCGLGGLGVVAEVTLQCV R ELVEHTY+
Sbjct: 241 DEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTYV 300

Query: 301 SNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQH 360
           SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK +PKYT+DEA+QH
Sbjct: 301 SNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQH 360

Query: 361 VRDLYVESLQKYSASD---------ERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEA 420
           VRDLY ES+ KY   D         E DI E SFTELRDKLLALDPLN  HV KVNQAEA
Sbjct: 361 VRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAEA 420

Query: 421 EFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKEN 480
           EFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEKE 
Sbjct: 421 EFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKEA 480

Query: 481 IPAPAPIEQRWTACSKSPMSPASSSAEDDIFSWVGIIMYLPTSDARQRKEITEEFFHYRH 540
           IPAPAPIEQRWTA SKSP+SPA S++EDDIFSWVGIIMYLPT+D RQRK+IT+EFFHYRH
Sbjct: 481 IPAPAPIEQRWTARSKSPISPAFSTSEDDIFSWVGIIMYLPTADPRQRKDITDEFFHYRH 540

Query: 541 LTQTLLWDQYSAFEHWAKIEVPRDKDELAALQARLRKRFPVDEYNKARRELDPNKILSSN 584
           LTQ  LWDQ+SA+EHWAKIE+P+DK+EL ALQAR+RKRFPVD YNKARRELDPN+ILS+N
Sbjct: 541 LTQKQLWDQFSAYEHWAKIEIPKDKEELEALQARIRKRFPVDAYNKARRELDPNRILSNN 600

BLAST of Carg21110 vs. TAIR 10
Match: AT3G47930.2 (L-galactono-1,4-lactone dehydrogenase )

HSP 1 Score: 744.2 bits (1920), Expect = 8.1e-215
Identity = 377/495 (76.16%), Postives = 412/495 (83.23%), Query Frame = 0

Query: 1   MLRLLSLRRSLHLHHRRPQFPPTVAISRPPLNPTRQFSTVS--------------PPPPS 60
           MLR L LRRS  + H      P+ +  R   +P R   T                PPPP+
Sbjct: 1   MLRSLLLRRS--VGHSLGTLSPSSSTIRSSFSPHRTLCTTGQTLTPPPPPPPRPPPPPPA 60

Query: 61  SSSESELRKYIGYGALVILCGAATYYSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT 120
           ++SE++ RKY GY AL I  G ATY+SFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT
Sbjct: 61  TASEAQFRKYAGYAALAIFSGVATYFSFPFPENAKHKKAQIFRYAPLPEDLHTVSNWSGT 120

Query: 121 HEVLTRVFHQPENLEQLEEVVKESNVKKARIRPVGSGLSPNGIGLSRMGMVNLALMDEVL 180
           HEV TR F+QPENL  LE +VKES+ KK RIRPVGSGLSPNGIGLSR GMVNLALMD+VL
Sbjct: 121 HEVQTRNFNQPENLADLEALVKESHEKKLRIRPVGSGLSPNGIGLSRSGMVNLALMDKVL 180

Query: 181 EVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQNFASIREQQIGGIIQVGAHGTGAKLPPI 240
           EVDKEKKRV VQAGIRVQQLVD IK+YGLTLQNFASIREQQIGGIIQVGAHGTGA+LPPI
Sbjct: 181 EVDKEKKRVTVQAGIRVQQLVDAIKDYGLTLQNFASIREQQIGGIIQVGAHGTGARLPPI 240

Query: 241 DEQVIAMKLVTPAKGTIEISREKDPELFYLARCGLGGLGVVAEVTLQCVERQELVEHTYI 300
           DEQVI+MKLVTPAKGTIE+SREKDPELF+LARCGLGGLGVVAEVTLQCV R ELVEHTY+
Sbjct: 241 DEQVISMKLVTPAKGTIELSREKDPELFHLARCGLGGLGVVAEVTLQCVARHELVEHTYV 300

Query: 301 SNMKDIKKNHKKLLADNKHVKYLYIPYTDAIVVVTCNPISKWRGPPKFQPKYTSDEAIQH 360
           SN+++IKKNHKKLL+ NKHVKYLYIPYTD +VVVTCNP+SKW GPPK +PKYT+DEA+QH
Sbjct: 301 SNLQEIKKNHKKLLSANKHVKYLYIPYTDTVVVVTCNPVSKWSGPPKDKPKYTTDEAVQH 360

Query: 361 VRDLYVESLQKYSASD---------ERDINEFSFTELRDKLLALDPLNKEHVVKVNQAEA 420
           VRDLY ES+ KY   D         E DI E SFTELRDKLLALDPLN  HV KVNQAEA
Sbjct: 361 VRDLYRESIVKYRVQDSGKKSPDSSEPDIQELSFTELRDKLLALDPLNDVHVAKVNQAEA 420

Query: 421 EFWRKSEGYRVGWSDEILGFDCGGQQWVSETCFPAGTLAKPNMKDIEYIEELKQLIEKEN 473
           EFW+KSEGYRVGWSDEILGFDCGGQQWVSE+CFPAGTLA P+MKD+EYIEELK+LIEKE 
Sbjct: 421 EFWKKSEGYRVGWSDEILGFDCGGQQWVSESCFPAGTLANPSMKDLEYIEELKKLIEKEA 480

BLAST of Carg21110 vs. TAIR 10
Match: AT2G19500.1 (cytokinin oxidase 2 )

HSP 1 Score: 49.3 bits (116), Expect = 1.2e-05
Identity = 53/222 (23.87%), Postives = 98/222 (44.14%), Query Frame = 0

Query: 141 GSGLSPNG-IGLSRMGMVNLALMDEVLEVDKEKKRVRVQAGIRVQQLVDGIKEYGLTLQN 200
           G G S NG   +S   +VN+  + +V+ V K+KK   V AG     ++    E G++  +
Sbjct: 89  GQGHSLNGQASVSGGVIVNMTCITDVV-VSKDKKYADVAAGTLWVDVLKKTAEKGVSPVS 148

Query: 201 FASIREQQIGGIIQVGAHGTGA-KLPPIDEQVIAMKLVTPAKGTIEISREKDPELFYLAR 260
           +       +GG +  G  G    +  P+   V+ + ++T     +  SR+ +PELFY   
Sbjct: 149 WTDYLHITVGGTLSNGGIGGQVFRNGPLVSNVLELDVITGKGEMLTCSRQLNPELFYGVL 208

Query: 261 CGLGGLGVV--AEVTLQCVERQELVEHTYISNMKDIKKNHKKLLADNKHVKYLYIPYTDA 320
            GLG  G++  A + L    ++        S+     K+ ++L++    +    + Y + 
Sbjct: 209 GGLGQFGIITRARIVLDHAPKRAKWFRMLYSDFTTFTKDQERLISMANDIG---VDYLEG 268

Query: 321 IVVVTCNPISKWRGPPKFQPKYTSDEAIQHVRDLYVESLQKY 359
            + ++   +     PP  Q K  +D   QH   +YV  + KY
Sbjct: 269 QIFLSNGVVDTSFFPPSDQSK-VADLVKQH-GIIYVLEVAKY 304

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7027658.10.0e+00100.00L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita argyrosperma sub... [more]
KAG6595695.10.0e+0099.66L-galactono-1,4-lactone dehydrogenase, mitochondrial, partial [Cucurbita argyros... [more]
XP_022925153.10.0e+0099.49L-galactono-1,4-lactone dehydrogenase, mitochondrial-like [Cucurbita moschata][more]
XP_023517263.10.0e+0098.30L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita pepo subsp. pepo... [more]
XP_022966248.10.0e+0097.11L-galactono-1,4-lactone dehydrogenase, mitochondrial [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9SU562.9e-27076.73L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Arabidopsis thaliana OX=... [more]
O478819.5e-26977.98L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Brassica oleracea OX=371... [more]
Q2RAP01.1e-24573.62L-galactono-1,4-lactone dehydrogenase 1, mitochondrial OS=Oryza sativa subsp. ja... [more]
Q2QXY15.6e-24573.26L-galactono-1,4-lactone dehydrogenase 2, mitochondrial OS=Oryza sativa subsp. ja... [more]
P108671.7e-3626.24L-gulonolactone oxidase OS=Rattus norvegicus OX=10116 GN=Gulo PE=1 SV=3[more]
Match NameE-valueIdentityDescription
A0A6J1EEF40.0e+0099.49L-galactono-1,4-lactone dehydrogenase, mitochondrial-like OS=Cucurbita moschata ... [more]
A0A6J1HRG80.0e+0097.11L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Cucurbita maxima OX=3661... [more]
E7E0160.0e+0092.03L-galactono-1,4-lactone dehydrogenase OS=Cucumis sativus OX=3659 PE=2 SV=1[more]
Q9M4U90.0e+0091.19L-galactono-1,4-lactone dehydrogenase OS=Cucumis melo OX=3656 GN=LOC103497260 PE... [more]
A0A6J1DK815.3e-30789.19L-galactono-1,4-lactone dehydrogenase, mitochondrial OS=Momordica charantia OX=3... [more]
Match NameE-valueIdentityDescription
AT3G47930.12.1e-27176.73L-galactono-1,4-lactone dehydrogenase [more]
AT3G47930.28.1e-21576.16L-galactono-1,4-lactone dehydrogenase [more]
AT2G19500.11.2e-0523.87cytokinin oxidase 2 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePIRSFPIRSF000136LGO_GLOcoord: 380..587
e-value: 5.0E-8
score: 30.0
coord: 95..375
e-value: 1.3E-51
score: 173.6
IPR010031L-gulonolactone/D-arabinono-1,4-lactone oxidasePANTHERPTHR43762L-GULONOLACTONE OXIDASEcoord: 93..584
IPR007173D-arabinono-1,4-lactone oxidasePFAMPF04030ALOcoord: 270..579
e-value: 2.8E-16
score: 60.0
IPR016169FAD-binding, type PCMH, subdomain 2GENE3D3.30.465.10coord: 165..292
e-value: 1.0E-28
score: 101.3
IPR006094FAD linked oxidase, N-terminalPFAMPF01565FAD_binding_4coord: 113..243
e-value: 4.2E-24
score: 84.9
IPR010029Galactonolactone dehydrogenaseTIGRFAMTIGR01676TIGR01676coord: 50..584
e-value: 0.0
score: 1201.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 29..51
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 18..51
NoneNo IPR availablePANTHERPTHR43762:SF1L-GULONOLACTONE OXIDASEcoord: 93..584
IPR016166FAD-binding domain, PCMH-typePROSITEPS51387FAD_PCMHcoord: 106..277
score: 17.056955
IPR036318FAD-binding, type PCMH-like superfamilySUPERFAMILY56176FAD-binding/transporter-associated domain-likecoord: 94..275

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg21110-RACarg21110-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019853 L-ascorbic acid biosynthetic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0016020 membrane
molecular_function GO:0003885 D-arabinono-1,4-lactone oxidase activity
molecular_function GO:0071949 FAD binding
molecular_function GO:0016633 galactonolactone dehydrogenase activity
molecular_function GO:0050660 flavin adenine dinucleotide binding
molecular_function GO:0016491 oxidoreductase activity
molecular_function GO:0016899 oxidoreductase activity, acting on the CH-OH group of donors, oxygen as acceptor