Carg20825 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20825
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionMitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein
LocationCarg_Chr15: 9609708 .. 9614133 (-)
RNA-Seq ExpressionCarg20825
SyntenyCarg20825
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
CTTCTTCCTCGTCTTCCTCAACCACCAATGGCTTAGTTTCCGCCATTTCCACCATTTCCACTATTTCCACTATTTCCACCGTTCTCTTTCTCATCTTCCTTCGCATCCCTCTCCTCCATCTCCGATCTCTCGTAATTCCTCCTTCTCTCTTCCTCAGATGCCCCCATTTCCCTTTCTCCACATCGCAAAATGTCATCCTCTTCCTCTTCCTCTTTCCCCCCCTTTTCCTCTCCCTCATCGCCGTCTCTGAACAGGAGCACCAGCACCATCCAGAATTCGGAGGCCGAGAACCGTCTCCGTGAGGCGGAGGACCAGCTCCGGGAAGCTATTGAGGAGCTTCAACGTAGGCAGCGGAAAGTTGCGGCATGTGGATCTCATCATCACCAGACCCGGGAGCAGCCCCCGCCGTGTGAACATGCTGCTTATGAGTCCTCTTGTGTGGCTAATGCCATTGGTAACCTCTGCCAGACCTTCCTTCTCTCTTATGGTGTTAGAGTTGGCATTGGTATACTTCTCCGTGCTTTCAAGCTCGCGCGGAGGCAGTCCTATTCCTCTATTCTTGATCTCAAGGTTCGTTTTGCTTGATGTCATTCGTGTTTTTAAATTGTTGATTCCGGTATTCCTTCTTGTTTTGTGAGATCGCCGATTGAAGTTTTGAATCAGGGCTGGAAAAGATTGTTGGATTCACGTCACCATACAGTTTTCTCCCTTTATTATTGTTTTTATGATCAATATAGTTGTTTCTTATGGCTGCCATGTGACTGTTTGTTTAGGTTAATGCATTTGATGGAGATTATTATAGTTCTTTTTCGTTGAATTGTATTCTTCACCATGTTTGTTGGGAATGAGTTCTTATTGGAGGTGAGTGAAAACGAAATGGGATGATTTTTCAGGGATTAAAAGAATATTTCCAATAGGAACAATCAAGATCGAAGTAAAGAGGGTTGTCATTTGTTTACCTCCATTTTTTTCCATATTAGGATTTAACTTTTTTCCCCCACAAGTGATTTATGTCCGACTGCGTTGTTATATGTTTGTATTGGTTCATTTTTTTTCAAATGAAAGAACATCTTACAACAATGGTTCTCTCTTCTATGAATTCTCTGCACTTTTTTTTCCCCCTAACAACGCCCTGTAAAAGTCGAGCTAGACTTTTTGAGTATGAAAGAGGCGTTATATCTCGTTTTTTGTGACGGTTTTATAAATTGGCTCGATCTATTTAGAAGTACAAAAATTCTTAGAGAGGTGGGAAAATTATGCTTTTTTGATAGATAAGTACAACAAGATAGGAGTGGTGATGGAAGTTAAAAGAAAGGTAGCGCTTTCATACTAGAGGGGCAAAACTTGGAAGGTTAATCTTTATGTAATGTGTTTAACTTTCTGCTGCCTCTCACATCTATTTAATAATTTAGTTCTTTGAGGATATATTGTTCTCTCCATTTTTTTTCATGTACTTGATTAGATTTTTCCTCCGGAACACTTTTCTGTAGCAACTTGTCTCAGAGAAAGACTTGATAGTGAGAGAAGAGGCATGTCGTGTTGGTTTACTCTTTGGTGGTTTTACTGGGTCTTATCATGCCCTTAGATGCTTGCTAAGAAAGTGTAGAAAGAAAGAGACATCGTTAAACGTGTAAGTCTCCAATCCATTGTAGCATCAACTTTCACGTCTTCATGATTCTGTTTGTGTTTCATTTTCTTCTTTTTAATGGATGATGAGCAGAATTTTAGCAGGTTCAGTGGCTGGTCTGTCTGTTTTAGCATTAGATGGCTCAAACCGGAGGCGTACTCTTGCTTTATATCTTATGGCTAGACTTGCTCAGGTACAAAGCGCACAAGTTTACCGTTGAGATGTAGCTTTCTTTCTCCCTTTTCTTTGGCTCTCTATCTTTATCTTTATAATTCTATCTGACTAGGTATTTCTTTTTACTTTCTTCATTTTCTTCCTAAAATTAGTGTGCCTATAATTCTGCAAAATCCAAGAACAAGTTCCACTTCTGGGGAAGTCACTGGAGACATGGTGATTCTCTTCTTTTTGCCATGGCATGTGCACAGGTAGTTTAAAGCTGTATGGGTTCTCTGCTTTCTATTTTTTGTGGAGCATTTGATTGGGGAGGAATAAAAAGTTCTTATTTTTTATTTTTTTTAATTTTTATTTTTATCATGTGATCGTACTCGTGCAGATGTTTCCGCTTAGGCTTTTGCCTTTTACATTATTTGTATTACTATTACTCCCGTTTGAATCATCAACAGTTGGGCCTCTTTTAATTCTACTTCCTTTGTTGGCCCTTGTTGATTGTGTTTCCCATTTTCTCAATAAAAGATCTCTTTTATATCAACCAAATATCTATCTATCTATCTATATATATAGTATATGTGTGTATCTCCTAGTCCCTCCAACACTACTGGTGCTGTTTTTGTAAGCTTCTATGGTTGATTTGCGGAATTTCTATTTCTTGATTAAAAGAACTATGGGATTCATCTCTGCCCTTATGATGTTTCTGGCATAGAATTTATATAATTGTGATAACCTCATACTAGTGATTAACTATTTGTTACAGGTTATGTATTCCTTTGTAATGCGTCCGGAGAGCTTGCCAAAAGCATATCGGGACTTCATTCAGATTACAGGGCCAGTTGCGGGACCTGTCTACAAGGCTGTACGAGATAGTTGTAGAGGCCATCCTGTGGATGGTGCTTCACTTTCTTCTTACTTGTCAGATAGGCAGAAATCTGGCTATGGAAATCTAGATGAATTTCCTTCCATTATTCCATGTAGTATAATCCATCCGGACACGAATTCGTGCATGGCTCACAATACGCAGGCAGCATCAGCCACATTCAAGAAGACATTTCCTCTTTACTTCTCTTTGACATTTGTACCATTCGTTGTTTTGCACCTGCAGAAAGTAAGTTCTCCCTAAAATTTTCTTCTACCAGGATTGGCCCACCAAAATTGTGTTCCTTGGTTAGAGCTGCCATTGTTTAGAATTGGTAGTTTCCCTTCTTTTTCCTTCACTAGGAAACAGAGGTCTTTCCATTAAAACTATGACACGTACATGAGAAAGATGTACAAAAACCAAGTAGTTACAAAAGCCTCTTATTATTTGATACGTGCATAGTTATATATATATTAAGAAGATTCTTGTCTTTTACTAAAATGATTAGATAGTTGTTATCCCCTGTATTTTTTGCTGAGATTTTTTTTTTCCCCATTAAGTTCTCGTTTCTAGTTATCATAATTCATGCTTTTGCAGTTCATGGATGCTCCGGGTCGTACCTGTTGGCTTGCTGTCAAAGGTGCAGTTCGCTCGACAAGTTTTTTATCTGCCTTTGTTGGGATTTTTCAGGTCAATGTTTTCTGATCTGTATTCACGTTAATATCAGTTGAAGTCTGTAAGATAACGTTCTGAAGTATTTGGGATGATGCTAGGCGGTCATTTGTTTGCATAGGAAAGTTGCAACCAAAGACCACAAACTCGTATATTGGGTTGCCGGTGGAATATCTGCCCTTTCAGTTCTTTTAGAGAAGAAGGGAAGACGATCTGAACTAGCTCTCTATGTTCTTCCGCGTGCAGCTGATTCTTGGTGGTATATTTTGGTCAACCGCCACCTGGTTCCAAACATAAAAAACGCTGAGGTATGATTACTTCAAAATCAAATGTAATCGTTAAAACTTAAAAGTTAACTGTTTCGAGTGCACATCAGTCTATCTATGTCCTGATCTTAAATATGTTGAGGATTATTGGGAGTGAGTCCCACGTTAGTTAATTTAGTTAATTTAGTGGAAGATCATAGGTTTATAAGTAAAGAATACTATCTCCATTGGTATGAGGTCTTTTGGGGAAGCCCAAAGCAAAGCCACGAGAGCTTATGCTCAAAGTGGACAATATCATATCTTTGTGGAGAATCGTGATTTCATCCAGCAATATACTTCCTCATATGTTAATGGCCTAGAATAACTGACAATGTCATGCTTGAAATCTAATTAATTGCCACGTATATCGATCCTCGAGAGAGATACTCATTTCCAAAATAGTTTCTATATTTGTGTGGAGGAGTTCTACAAAATCTCTACAGTGTATTAGGATCGTAAACAACCTGATACCTATGATGTTCTGTTAAATTTCTAACACTTTTCCTTTGTTTTGGTAATATGAGCCAGGTCTTCCTGTTCTCAATTTGTATGGGAGGACTCATGTATTACTTAGAATATGAGCCAGATACCATGGCTCCATTCCTCCGAGGTCTGATTCGTCGCTTCCTCGCGAGCAGAATCAGCAATTCTGGCATATCTTCCAGTCGAGATGTTTCACACTCTTATATAAACACACTCGACACCATGAAGAAACCAAATGTGGAAGATAGCGAGGAAGTCGAAGCTGCTCGGTCTCAGAAGTACAACCTCGAATCGATTCCAGGTTTATAG

mRNA sequence

CTTCTTCCTCGTCTTCCTCAACCACCAATGGCTTAGTTTCCGCCATTTCCACCATTTCCACTATTTCCACTATTTCCACCGTTCTCTTTCTCATCTTCCTTCGCATCCCTCTCCTCCATCTCCGATCTCTCGTAATTCCTCCTTCTCTCTTCCTCAGATCGCAAAATGTCATCCTCTTCCTCTTCCTCTTTCCCCCCCTTTTCCTCTCCCTCATCGCCGTCTCTGAACAGGAGCACCAGCACCATCCAGAATTCGGAGGCCGAGAACCGTCTCCGTGAGGCGGAGGACCAGCTCCGGGAAGCTATTGAGGAGCTTCAACGTAGGCAGCGGAAAGTTGCGGCATGTGGATCTCATCATCACCAGACCCGGGAGCAGCCCCCGCCGTGTGAACATGCTGCTTATGAGTCCTCTTGTGTGGCTAATGCCATTGGTAACCTCTGCCAGACCTTCCTTCTCTCTTATGGTGTTAGAGTTGGCATTGGTATACTTCTCCGTGCTTTCAAGCTCGCGCGGAGGCAGTCCTATTCCTCTATTCTTGATCTCAAGCAACTTGTCTCAGAGAAAGACTTGATAGTGAGAGAAGAGGCATGTCGTGTTGGTTTACTCTTTGGTGGTTTTACTGGGTCTTATCATGCCCTTAGATGCTTGCTAAGAAAGTGTAGAAAGAAAGAGACATCGTTAAACGTAATTTTAGCAGGTTCAGTGGCTGGTCTGTCTGTTTTAGCATTAGATGGCTCAAACCGGAGGCGTACTCTTGCTTTATATCTTATGGCTAGACTTGCTCAGTGTGCCTATAATTCTGCAAAATCCAAGAACAAGTTCCACTTCTGGGGAAGTCACTGGAGACATGGTGATTCTCTTCTTTTTGCCATGGCATGTGCACAGGTTATGTATTCCTTTGTAATGCGTCCGGAGAGCTTGCCAAAAGCATATCGGGACTTCATTCAGATTACAGGGCCAGTTGCGGGACCTGTCTACAAGGCTGTACGAGATAGTTGTAGAGGCCATCCTGTGGATGGTGCTTCACTTTCTTCTTACTTGTCAGATAGGCAGAAATCTGGCTATGGAAATCTAGATGAATTTCCTTCCATTATTCCATGTAGTATAATCCATCCGGACACGAATTCGTGCATGGCTCACAATACGCAGGCAGCATCAGCCACATTCAAGAAGACATTTCCTCTTTACTTCTCTTTGACATTTGTACCATTCGTTGTTTTGCACCTGCAGAAATTCATGGATGCTCCGGGTCGTACCTGTTGGCTTGCTGTCAAAGGTGCAGTTCGCTCGACAAGTTTTTTATCTGCCTTTGTTGGGATTTTTCAGGCGGTCATTTGTTTGCATAGGAAAGTTGCAACCAAAGACCACAAACTCGTATATTGGGTTGCCGGTGGAATATCTGCCCTTTCAGTTCTTTTAGAGAAGAAGGGAAGACGATCTGAACTAGCTCTCTATGTTCTTCCGCGTGCAGCTGATTCTTGGTGGTATATTTTGGTCAACCGCCACCTGGTTCCAAACATAAAAAACGCTGAGGTCTTCCTGTTCTCAATTTGTATGGGAGGACTCATGTATTACTTAGAATATGAGCCAGATACCATGGCTCCATTCCTCCGAGGTCTGATTCGTCGCTTCCTCGCGAGCAGAATCAGCAATTCTGGCATATCTTCCAGTCGAGATGTTTCACACTCTTATATAAACACACTCGACACCATGAAGAAACCAAATGTGGAAGATAGCGAGGAAGTCGAAGCTGCTCGGTCTCAGAAGTACAACCTCGAATCGATTCCAGGTTTATAG

Coding sequence (CDS)

ATGTCATCCTCTTCCTCTTCCTCTTTCCCCCCCTTTTCCTCTCCCTCATCGCCGTCTCTGAACAGGAGCACCAGCACCATCCAGAATTCGGAGGCCGAGAACCGTCTCCGTGAGGCGGAGGACCAGCTCCGGGAAGCTATTGAGGAGCTTCAACGTAGGCAGCGGAAAGTTGCGGCATGTGGATCTCATCATCACCAGACCCGGGAGCAGCCCCCGCCGTGTGAACATGCTGCTTATGAGTCCTCTTGTGTGGCTAATGCCATTGGTAACCTCTGCCAGACCTTCCTTCTCTCTTATGGTGTTAGAGTTGGCATTGGTATACTTCTCCGTGCTTTCAAGCTCGCGCGGAGGCAGTCCTATTCCTCTATTCTTGATCTCAAGCAACTTGTCTCAGAGAAAGACTTGATAGTGAGAGAAGAGGCATGTCGTGTTGGTTTACTCTTTGGTGGTTTTACTGGGTCTTATCATGCCCTTAGATGCTTGCTAAGAAAGTGTAGAAAGAAAGAGACATCGTTAAACGTAATTTTAGCAGGTTCAGTGGCTGGTCTGTCTGTTTTAGCATTAGATGGCTCAAACCGGAGGCGTACTCTTGCTTTATATCTTATGGCTAGACTTGCTCAGTGTGCCTATAATTCTGCAAAATCCAAGAACAAGTTCCACTTCTGGGGAAGTCACTGGAGACATGGTGATTCTCTTCTTTTTGCCATGGCATGTGCACAGGTTATGTATTCCTTTGTAATGCGTCCGGAGAGCTTGCCAAAAGCATATCGGGACTTCATTCAGATTACAGGGCCAGTTGCGGGACCTGTCTACAAGGCTGTACGAGATAGTTGTAGAGGCCATCCTGTGGATGGTGCTTCACTTTCTTCTTACTTGTCAGATAGGCAGAAATCTGGCTATGGAAATCTAGATGAATTTCCTTCCATTATTCCATGTAGTATAATCCATCCGGACACGAATTCGTGCATGGCTCACAATACGCAGGCAGCATCAGCCACATTCAAGAAGACATTTCCTCTTTACTTCTCTTTGACATTTGTACCATTCGTTGTTTTGCACCTGCAGAAATTCATGGATGCTCCGGGTCGTACCTGTTGGCTTGCTGTCAAAGGTGCAGTTCGCTCGACAAGTTTTTTATCTGCCTTTGTTGGGATTTTTCAGGCGGTCATTTGTTTGCATAGGAAAGTTGCAACCAAAGACCACAAACTCGTATATTGGGTTGCCGGTGGAATATCTGCCCTTTCAGTTCTTTTAGAGAAGAAGGGAAGACGATCTGAACTAGCTCTCTATGTTCTTCCGCGTGCAGCTGATTCTTGGTGGTATATTTTGGTCAACCGCCACCTGGTTCCAAACATAAAAAACGCTGAGGTCTTCCTGTTCTCAATTTGTATGGGAGGACTCATGTATTACTTAGAATATGAGCCAGATACCATGGCTCCATTCCTCCGAGGTCTGATTCGTCGCTTCCTCGCGAGCAGAATCAGCAATTCTGGCATATCTTCCAGTCGAGATGTTTCACACTCTTATATAAACACACTCGACACCATGAAGAAACCAAATGTGGAAGATAGCGAGGAAGTCGAAGCTGCTCGGTCTCAGAAGTACAACCTCGAATCGATTCCAGGTTTATAG

Protein sequence

MSSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACGSHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYSSILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVAGLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQVMYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYGNLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAPGRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKKGRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPFLRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIPGL
Homology
BLAST of Carg20825 vs. NCBI nr
Match: KAG6579643.1 (Transmembrane protein 135, partial [Cucurbita argyrosperma subsp. sororia] >KAG7017098.1 Transmembrane protein [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1070.1 bits (2766), Expect = 6.3e-309
Identity = 543/543 (100.00%), Postives = 543/543 (100.00%), Query Frame = 0

Query: 1   MSSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAAC 60
           MSSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAAC
Sbjct: 1   MSSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAAC 60

Query: 61  GSHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSY 120
           GSHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSY
Sbjct: 61  GSHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSY 120

Query: 121 SSILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSV 180
           SSILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSV
Sbjct: 121 SSILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSV 180

Query: 181 AGLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQ 240
           AGLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQ
Sbjct: 181 AGLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQ 240

Query: 241 VMYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGY 300
           VMYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGY
Sbjct: 241 VMYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGY 300

Query: 301 GNLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDA 360
           GNLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDA
Sbjct: 301 GNLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDA 360

Query: 361 PGRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEK 420
           PGRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEK
Sbjct: 361 PGRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEK 420

Query: 421 KGRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAP 480
           KGRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAP
Sbjct: 421 KGRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAP 480

Query: 481 FLRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESI 540
           FLRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESI
Sbjct: 481 FLRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESI 540

Query: 541 PGL 544
           PGL
Sbjct: 541 PGL 543

BLAST of Carg20825 vs. NCBI nr
Match: XP_022928778.1 (uncharacterized protein LOC111435600 [Cucurbita moschata])

HSP 1 Score: 1057.7 bits (2734), Expect = 3.3e-305
Identity = 538/542 (99.26%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG
Sbjct: 7   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 66

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTRE+PP CEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 67  SHHHQTRERPPTCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 126

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA
Sbjct: 127 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 186

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV
Sbjct: 187 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 246

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAY DFIQITGPVA PVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG
Sbjct: 247 MYSFVMRPESLPKAYLDFIQITGPVAEPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 306

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 307 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 366

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 367 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 426

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF
Sbjct: 427 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 486

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP
Sbjct: 487 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 546

Query: 542 GL 544
           GL
Sbjct: 547 GL 548

BLAST of Carg20825 vs. NCBI nr
Match: XP_023549889.1 (uncharacterized protein LOC111808251 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1053.5 bits (2723), Expect = 6.2e-304
Identity = 535/542 (98.71%), Postives = 538/542 (99.26%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPPFSSPSSPSLNRSTS+IQNSEAENRLREAEDQLREAIEELQRRQRKVAACG
Sbjct: 8   SSSSSSSFPPFSSPSSPSLNRSTSSIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 67

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 68  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 127

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA
Sbjct: 128 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 187

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV
Sbjct: 188 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 247

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAYRDFIQITGPVA PVYKAVRD CRGHPVDGASLSSYLSDRQKSGYG
Sbjct: 248 MYSFVMRPESLPKAYRDFIQITGPVAEPVYKAVRDICRGHPVDGASLSSYLSDRQKSGYG 307

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 308 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 367

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 368 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 427

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF
Sbjct: 428 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 487

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNS IS+S DVSHSYINTLDTMKKPNVEDSEEV+AARSQKYNLESIP
Sbjct: 488 LRGLIRRFLASRISNSSISASPDVSHSYINTLDTMKKPNVEDSEEVKAARSQKYNLESIP 547

Query: 542 GL 544
           GL
Sbjct: 548 GL 549

BLAST of Carg20825 vs. NCBI nr
Match: XP_022969748.1 (uncharacterized protein LOC111468853 [Cucurbita maxima] >XP_022969749.1 uncharacterized protein LOC111468853 [Cucurbita maxima])

HSP 1 Score: 1048.1 bits (2709), Expect = 2.6e-302
Identity = 532/542 (98.15%), Postives = 537/542 (99.08%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPPFSSPSSPSLNRSTS+IQNSEAENRLREAEDQLREAIEELQRRQRKVAACG
Sbjct: 8   SSSSSSSFPPFSSPSSPSLNRSTSSIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 67

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 68  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 127

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKK+TSLNVILAGSVA
Sbjct: 128 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKDTSLNVILAGSVA 187

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV
Sbjct: 188 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 247

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAYRDFIQ TGPVA PVYKAVRDSCRGHPVDGASLSSYLSDRQKSGY 
Sbjct: 248 MYSFVMRPESLPKAYRDFIQNTGPVAEPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYE 307

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NLDEFPSIIPC+IIHPDT+SCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 308 NLDEFPSIIPCNIIHPDTDSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 367

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
            RTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 368 SRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 427

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRAADSWWYILV RHLVPNIKNAEVFLFSICMGG+MYYLEYEPDTMAPF
Sbjct: 428 GRRSELALYVLPRAADSWWYILVKRHLVPNIKNAEVFLFSICMGGIMYYLEYEPDTMAPF 487

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP
Sbjct: 488 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 547

Query: 542 GL 544
           GL
Sbjct: 548 GL 549

BLAST of Carg20825 vs. NCBI nr
Match: XP_038874369.1 (uncharacterized protein LOC120067061 [Benincasa hispida])

HSP 1 Score: 986.9 bits (2550), Expect = 7.1e-284
Identity = 499/542 (92.07%), Postives = 518/542 (95.57%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPP SS  SPSL RS S+IQNSEAE RLREAED+LREAIEELQRRQRKVAACG
Sbjct: 10  SSSSSSSFPPLSSSPSPSLQRSISSIQNSEAERRLREAEDRLREAIEELQRRQRKVAACG 69

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTREQPPPC+HAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 70  SHHHQTREQPPPCDHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 129

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACR+GLLFGGFTGSYHALRCLLRK RK+ET  NVILAGSVA
Sbjct: 130 SILDLKQLVSEKDLIVREEACRIGLLFGGFTGSYHALRCLLRKWRKRETPFNVILAGSVA 189

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALD SNRRRTLALYLMARLAQCAYNSAKSKNKFH WGSHWRHGDSLLFAMACAQV
Sbjct: 190 GLSVLALDDSNRRRTLALYLMARLAQCAYNSAKSKNKFHLWGSHWRHGDSLLFAMACAQV 249

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPK+YRDFIQ TGPVA PVYKAVRDSCRG+PVD ASLSSYLS RQ +GY 
Sbjct: 250 MYSFVMRPESLPKSYRDFIQKTGPVAEPVYKAVRDSCRGYPVDVASLSSYLSRRQNTGYE 309

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NL+EFPSIIPC+IIHPDTNSC+AHN +AASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 310 NLEEFPSIIPCNIIHPDTNSCLAHNGKAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 369

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQ VICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 370 GRTCWLAVKGAVRSTSFLSAFVGIFQVVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 429

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRA DSWWYILVNRHL+PNIKNAEVFLFSICMGG+MYYLEYEPDTMAPF
Sbjct: 430 GRRSELALYVLPRAVDSWWYILVNRHLLPNIKNAEVFLFSICMGGIMYYLEYEPDTMAPF 489

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNS  +SSRDVSHSY+NTLD MKKPN+ED+ EVEAARS+KYNLESIP
Sbjct: 490 LRGLIRRFLASRISNSSTTSSRDVSHSYLNTLDAMKKPNLEDNREVEAARSEKYNLESIP 549

Query: 542 GL 544
           GL
Sbjct: 550 GL 551

BLAST of Carg20825 vs. ExPASy Swiss-Prot
Match: Q86UB9 (Transmembrane protein 135 OS=Homo sapiens OX=9606 GN=TMEM135 PE=2 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 2.5e-05
Identity = 31/103 (30.10%), Postives = 55/103 (53.40%), Query Frame = 0

Query: 373 VRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISAL-----SVLLEKKGRRSEL 432
           ++S SFL+A   ++ A  C+ RK+  K +    W  G  +AL     ++L+E+K RR  L
Sbjct: 69  LQSASFLTANGALYMAFFCILRKILGKFYS---WTPGFGAALPASYVAILIERKSRRGLL 128

Query: 433 ALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYY 471
            +Y+   A ++ + + V R  +  ++N EV LF I     M++
Sbjct: 129 TIYMANLATETLFRMGVARGTITTLRNGEVLLFCITAAMYMFF 168

BLAST of Carg20825 vs. ExPASy Swiss-Prot
Match: Q3ZBE6 (Transmembrane protein 135 OS=Bos taurus OX=9913 GN=TMEM135 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.2e-05
Identity = 32/103 (31.07%), Postives = 55/103 (53.40%), Query Frame = 0

Query: 373 VRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISAL-----SVLLEKKGRRSEL 432
           ++S SFL+A   +F A  C+ RK+     K   W  G  +AL     ++L+E+K RR  L
Sbjct: 69  LQSASFLTANGALFMAFFCILRKIL---GKFYLWSPGFGAALPASYVAILVERKSRRGLL 128

Query: 433 ALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYY 471
            +Y+   A ++ + + V R ++  ++N EV LF I     M++
Sbjct: 129 TIYMANLATETLFRMGVARGVITTLRNGEVLLFCITAAMYMFF 168

BLAST of Carg20825 vs. ExPASy Swiss-Prot
Match: Q6GQ39 (Transmembrane protein 135 OS=Xenopus laevis OX=8355 GN=tmem135 PE=2 SV=1)

HSP 1 Score: 51.6 bits (122), Expect = 3.2e-05
Identity = 41/160 (25.62%), Postives = 76/160 (47.50%), Query Frame = 0

Query: 316 HPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAPGRTCWLAVKGAVRS 375
           H    SC     Q      +++F +Y  L  V  ++   +K +D         +   ++S
Sbjct: 17  HTWNPSCFGSYLQITQGAMEESFKIYAPLYLVAAIL--RRKNLD---YYVHKLLPELLQS 76

Query: 376 TSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISAL-----SVLLEKKGRRSELALY 435
           TSFL+A   ++ A  C+ RK+     +  +W  G  +AL     ++L+E+K RR  L +Y
Sbjct: 77  TSFLTANGSLYIAFFCILRKLL---GRFYFWTPGFGAALPASYAAILIERKSRRGLLTIY 136

Query: 436 VLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYY 471
           +  +A ++ + + V R  +  I++ EV LF I     M++
Sbjct: 137 MANQATEALFRMGVTRGYIKPIRHGEVLLFCITSALYMFF 168

BLAST of Carg20825 vs. ExPASy Swiss-Prot
Match: Q9CYV5 (Transmembrane protein 135 OS=Mus musculus OX=10090 GN=Tmem135 PE=2 SV=1)

HSP 1 Score: 49.3 bits (116), Expect = 1.6e-04
Identity = 30/103 (29.13%), Postives = 54/103 (52.43%), Query Frame = 0

Query: 373 VRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISAL-----SVLLEKKGRRSEL 432
           ++S SFL+A   ++    C+ RK+  K +    W  G  +AL     ++L+E+K RR  L
Sbjct: 69  LQSASFLTANGALYITFFCILRKILGKFYS---WTPGFGAALPASYVAILIERKSRRGLL 128

Query: 433 ALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYY 471
            +Y+   A ++ + + V R  +  ++N EV LF I     M++
Sbjct: 129 TIYMANLATETLFRMGVARGTITTLRNGEVLLFCITAAMYMFF 168

BLAST of Carg20825 vs. ExPASy Swiss-Prot
Match: Q5U4F4 (Transmembrane protein 135 OS=Rattus norvegicus OX=10116 GN=Tmem135 PE=2 SV=1)

HSP 1 Score: 48.9 bits (115), Expect = 2.1e-04
Identity = 30/103 (29.13%), Postives = 54/103 (52.43%), Query Frame = 0

Query: 373 VRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISAL-----SVLLEKKGRRSEL 432
           ++S SFL+A   ++    C+ RK+  K +    W  G  +AL     ++L+E+K RR  L
Sbjct: 69  LQSASFLTANGALYITFFCVLRKILGKFYS---WTPGFGAALPASYVAILIERKSRRGLL 128

Query: 433 ALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYY 471
            +Y+   A ++ + + V R  +  ++N EV LF I     M++
Sbjct: 129 TIYMANLATETLFRMGVARGTITTLRNGEVLLFCITAAMYMFF 168

BLAST of Carg20825 vs. ExPASy TrEMBL
Match: A0A6J1EQ31 (uncharacterized protein LOC111435600 OS=Cucurbita moschata OX=3662 GN=LOC111435600 PE=3 SV=1)

HSP 1 Score: 1057.7 bits (2734), Expect = 1.6e-305
Identity = 538/542 (99.26%), Postives = 539/542 (99.45%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG
Sbjct: 7   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 66

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTRE+PP CEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 67  SHHHQTRERPPTCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 126

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA
Sbjct: 127 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 186

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV
Sbjct: 187 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 246

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAY DFIQITGPVA PVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG
Sbjct: 247 MYSFVMRPESLPKAYLDFIQITGPVAEPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 306

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 307 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 366

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 367 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 426

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF
Sbjct: 427 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 486

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP
Sbjct: 487 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 546

Query: 542 GL 544
           GL
Sbjct: 547 GL 548

BLAST of Carg20825 vs. ExPASy TrEMBL
Match: A0A6J1I3K4 (uncharacterized protein LOC111468853 OS=Cucurbita maxima OX=3661 GN=LOC111468853 PE=3 SV=1)

HSP 1 Score: 1048.1 bits (2709), Expect = 1.3e-302
Identity = 532/542 (98.15%), Postives = 537/542 (99.08%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPPFSSPSSPSLNRSTS+IQNSEAENRLREAEDQLREAIEELQRRQRKVAACG
Sbjct: 8   SSSSSSSFPPFSSPSSPSLNRSTSSIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 67

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 68  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 127

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKK+TSLNVILAGSVA
Sbjct: 128 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKDTSLNVILAGSVA 187

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV
Sbjct: 188 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 247

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAYRDFIQ TGPVA PVYKAVRDSCRGHPVDGASLSSYLSDRQKSGY 
Sbjct: 248 MYSFVMRPESLPKAYRDFIQNTGPVAEPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYE 307

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NLDEFPSIIPC+IIHPDT+SCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 308 NLDEFPSIIPCNIIHPDTDSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 367

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
            RTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 368 SRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 427

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRAADSWWYILV RHLVPNIKNAEVFLFSICMGG+MYYLEYEPDTMAPF
Sbjct: 428 GRRSELALYVLPRAADSWWYILVKRHLVPNIKNAEVFLFSICMGGIMYYLEYEPDTMAPF 487

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP
Sbjct: 488 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 547

Query: 542 GL 544
           GL
Sbjct: 548 GL 549

BLAST of Carg20825 vs. ExPASy TrEMBL
Match: A0A5A7UIM2 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein isoform 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold231G00260 PE=4 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 2.1e-281
Identity = 494/542 (91.14%), Postives = 517/542 (95.39%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPP SS  SPSL RS+S+IQNSEAE RLREAED+LREAIEELQRRQRKVAACG
Sbjct: 11  SSSSSSSFPPLSSSQSPSLQRSSSSIQNSEAERRLREAEDRLREAIEELQRRQRKVAACG 70

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SH HQTREQPPPC+HAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 71  SHRHQTREQPPPCDHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 130

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACR+GLLFGGFTGSYHALRC LRK RK+ET  NV+LAGSVA
Sbjct: 131 SILDLKQLVSEKDLIVREEACRIGLLFGGFTGSYHALRCFLRKWRKRETPFNVVLAGSVA 190

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALD SNRRRTLALYLMARLAQCAYNSAKSKNKFH WGSHWRHGDSLLFAMACAQV
Sbjct: 191 GLSVLALDDSNRRRTLALYLMARLAQCAYNSAKSKNKFHLWGSHWRHGDSLLFAMACAQV 250

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAYRDFIQ TGPVA PVYKAVRDSCRG+PVD A+LSSYLS RQ +GY 
Sbjct: 251 MYSFVMRPESLPKAYRDFIQKTGPVAEPVYKAVRDSCRGYPVDVAALSSYLSRRQNNGYE 310

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NL+EFPSIIPCSIIHPDT SC++HN +AASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 311 NLEEFPSIIPCSIIHPDTKSCLSHNGKAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 370

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 371 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 430

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRA DSWWYILVNRHL+PNIKNAEVFLF+ICMGG+MYYLEYEPDTMAPF
Sbjct: 431 GRRSELALYVLPRAVDSWWYILVNRHLLPNIKNAEVFLFAICMGGIMYYLEYEPDTMAPF 490

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNS  S+SRDVS+SY+NTLD MKKPN+ED+ EVEAARS+KYNLESIP
Sbjct: 491 LRGLIRRFLASRISNSTTSTSRDVSNSYLNTLDAMKKPNLEDNREVEAARSEKYNLESIP 550

Query: 542 GL 544
           GL
Sbjct: 551 GL 552

BLAST of Carg20825 vs. ExPASy TrEMBL
Match: A0A1S3CT92 (uncharacterized protein LOC103504699 OS=Cucumis melo OX=3656 GN=LOC103504699 PE=4 SV=1)

HSP 1 Score: 977.6 bits (2526), Expect = 2.1e-281
Identity = 494/542 (91.14%), Postives = 517/542 (95.39%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPP SS  SPSL RS+S+IQNSEAE RLREAED+LREAIEELQRRQRKVAACG
Sbjct: 10  SSSSSSSFPPLSSSQSPSLQRSSSSIQNSEAERRLREAEDRLREAIEELQRRQRKVAACG 69

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SH HQTREQPPPC+HAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 70  SHRHQTREQPPPCDHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 129

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SILDLKQLVSEKDLIVREEACR+GLLFGGFTGSYHALRC LRK RK+ET  NV+LAGSVA
Sbjct: 130 SILDLKQLVSEKDLIVREEACRIGLLFGGFTGSYHALRCFLRKWRKRETPFNVVLAGSVA 189

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALD SNRRRTLALYLMARLAQCAYNSAKSKNKFH WGSHWRHGDSLLFAMACAQV
Sbjct: 190 GLSVLALDDSNRRRTLALYLMARLAQCAYNSAKSKNKFHLWGSHWRHGDSLLFAMACAQV 249

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAYRDFIQ TGPVA PVYKAVRDSCRG+PVD A+LSSYLS RQ +GY 
Sbjct: 250 MYSFVMRPESLPKAYRDFIQKTGPVAEPVYKAVRDSCRGYPVDVAALSSYLSRRQNNGYE 309

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NL+EFPSIIPCSIIHPDT SC++HN +AASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 310 NLEEFPSIIPCSIIHPDTKSCLSHNGKAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 369

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 370 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 429

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRA DSWWYILVNRHL+PNIKNAEVFLF+ICMGG+MYYLEYEPDTMAPF
Sbjct: 430 GRRSELALYVLPRAVDSWWYILVNRHLLPNIKNAEVFLFAICMGGIMYYLEYEPDTMAPF 489

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNS  S+SRDVS+SY+NTLD MKKPN+ED+ EVEAARS+KYNLESIP
Sbjct: 490 LRGLIRRFLASRISNSTTSTSRDVSNSYLNTLDAMKKPNLEDNREVEAARSEKYNLESIP 549

Query: 542 GL 544
           GL
Sbjct: 550 GL 551

BLAST of Carg20825 vs. ExPASy TrEMBL
Match: A0A0A0KRJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G168790 PE=4 SV=1)

HSP 1 Score: 971.8 bits (2511), Expect = 1.1e-279
Identity = 492/542 (90.77%), Postives = 515/542 (95.02%), Query Frame = 0

Query: 2   SSSSSSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACG 61
           SSSSSSSFPP SS  SPSL RS S+IQNSEAE RLREAED+LREAIEELQRRQRKVAACG
Sbjct: 9   SSSSSSSFPPLSSSQSPSLQRSASSIQNSEAERRLREAEDRLREAIEELQRRQRKVAACG 68

Query: 62  SHHHQTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 121
           SHHHQTRE+PPPC+HAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS
Sbjct: 69  SHHHQTREKPPPCDHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYS 128

Query: 122 SILDLKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVA 181
           SI+DLKQLVSEKDLIVREEACR+GLLFGGFTGSYHALRCLLRK RK+ET  NV+LAGSVA
Sbjct: 129 SIIDLKQLVSEKDLIVREEACRIGLLFGGFTGSYHALRCLLRKWRKRETPFNVVLAGSVA 188

Query: 182 GLSVLALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQV 241
           GLSVLALD SNRRRTLALYLMARLAQCAYNSAKSKNKFH WGSHWRHGDSLLFAMACAQV
Sbjct: 189 GLSVLALDDSNRRRTLALYLMARLAQCAYNSAKSKNKFHLWGSHWRHGDSLLFAMACAQV 248

Query: 242 MYSFVMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYG 301
           MYSFVMRPESLPKAYRDFIQ TGPVA PVYKAVRDSCRG+PVD A+LSSYLS RQ +GY 
Sbjct: 249 MYSFVMRPESLPKAYRDFIQKTGPVAEPVYKAVRDSCRGYPVDVAALSSYLSRRQITGYE 308

Query: 302 NLDEFPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 361
           NL+E PSIIPCSIIHP+T SC+AHN +AASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP
Sbjct: 309 NLEELPSIIPCSIIHPNTKSCLAHNGKAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAP 368

Query: 362 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 421
           GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK
Sbjct: 369 GRTCWLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKK 428

Query: 422 GRRSELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPF 481
           GRRSELALYVLPRA DSWWYILVNRHL+PNIKNAEVFLF+ICMGG+MYYLEYEPDTMAPF
Sbjct: 429 GRRSELALYVLPRAVDSWWYILVNRHLLPNIKNAEVFLFAICMGGIMYYLEYEPDTMAPF 488

Query: 482 LRGLIRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARSQKYNLESIP 541
           LRGLIRRFLASRISNS  SSSRDVS+SY+NTL  MKKPN+ED+ E EAARS+KYNLESIP
Sbjct: 489 LRGLIRRFLASRISNSTTSSSRDVSNSYLNTLGAMKKPNLEDNREAEAARSKKYNLESIP 548

Query: 542 GL 544
           GL
Sbjct: 549 GL 550

BLAST of Carg20825 vs. TAIR 10
Match: AT5G51150.1 (Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein )

HSP 1 Score: 751.1 bits (1938), Expect = 6.1e-217
Identity = 393/539 (72.91%), Postives = 447/539 (82.93%), Query Frame = 0

Query: 6   SSSFPPFSSPSSPSLNRSTSTIQNSEAENRLREAEDQLREAIEELQRRQRKVAACGSHHH 65
           SS   P   PSS     S  +  +SEAE RLREAE++LR+A+ ELQRRQR  AA GSH  
Sbjct: 3   SSFSKPDLDPSSSPPPISPLSQDSSEAERRLREAEERLRDAMAELQRRQRS-AARGSHAE 62

Query: 66  QTREQPPPCEHAAYESSCVANAIGNLCQTFLLSYGVRVGIGILLRAFKLARRQSYSSILD 125
                   C+HA  + SCVANAIGNLCQ+FLLSYGVRVGIGILLRAFKLAR QSYSS+LD
Sbjct: 63  L-------CDHA--DVSCVANAIGNLCQSFLLSYGVRVGIGILLRAFKLARGQSYSSLLD 122

Query: 126 LKQLVSEKDLIVREEACRVGLLFGGFTGSYHALRCLLRKCRKKETSLNVILAGSVAGLSV 185
           LKQLVSEKDLIVREEACR+GLLFGGFTGSYHALRC LRK RKKET LN +LAGSVAGLS+
Sbjct: 123 LKQLVSEKDLIVREEACRIGLLFGGFTGSYHALRCCLRKWRKKETPLNSVLAGSVAGLSI 182

Query: 186 LALDGSNRRRTLALYLMARLAQCAYNSAKSKNKFHFWGSHWRHGDSLLFAMACAQVMYSF 245
           LALD SN+RRTLALYL+ARL Q AYNSAKSKNKFH WGSHWRHGDSLLF++ACAQVMYSF
Sbjct: 183 LALDDSNQRRTLALYLLARLGQAAYNSAKSKNKFHLWGSHWRHGDSLLFSLACAQVMYSF 242

Query: 246 VMRPESLPKAYRDFIQITGPVAGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYGNLDE 305
           +MRPE+LPK+YR+FIQ TGPVA PVY+AVR+ CRG P+D ASLS+Y+S + ++    ++E
Sbjct: 243 IMRPETLPKSYREFIQKTGPVARPVYQAVRECCRGGPIDVASLSAYISSKNEASDVKVEE 302

Query: 306 FPSIIPCSIIHPDTNSCMAHNTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAPGRTC 365
           F SIIPC+ IHP+TNSC+A N  A SATFKKTFPLYFSLTFVP+VVLHLQKFM +P RT 
Sbjct: 303 FASIIPCAAIHPNTNSCLAQNANAMSATFKKTFPLYFSLTFVPYVVLHLQKFMASPYRTS 362

Query: 366 WLAVKGAVRSTSFLSAFVGIFQAVICLHRKVATKDHKLVYWVAGGISALSVLLEKKGRRS 425
           WLA++ +VRSTSFLSAFVGIFQA IC HRKVATKDHKLVYW AGG +ALSV+LEKK RRS
Sbjct: 363 WLAIRDSVRSTSFLSAFVGIFQAFICAHRKVATKDHKLVYWFAGGAAALSVMLEKKPRRS 422

Query: 426 ELALYVLPRAADSWWYILVNRHLVPNIKNAEVFLFSICMGGLMYYLEYEPDTMAPFLRGL 485
           ELALYVLPRA DS W ILVNRHL+P+IKNAEV LF  CMGG+MYYLEYEPDTMAPFLRGL
Sbjct: 423 ELALYVLPRAGDSLWEILVNRHLLPDIKNAEVALFCGCMGGIMYYLEYEPDTMAPFLRGL 482

Query: 486 IRRFLASRISNSGISSSRDVSHSYINTLDTMKKPNVEDSEEVEAARS-QKYNLESIPGL 544
           IRRFLAS+ISN         S+SY+ TLD +KKP  ++S E E  ++ +KYNLE+IPGL
Sbjct: 483 IRRFLASQISNPSSKYPHSSSYSYLQTLDALKKPKTQESREGETPKAEEKYNLEAIPGL 531

BLAST of Carg20825 vs. TAIR 10
Match: AT1G34630.1 (BEST Arabidopsis thaliana protein match is: Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein (TAIR:AT5G51150.1); Has 323 Blast hits to 315 proteins in 124 species: Archae - 0; Bacteria - 0; Metazoa - 95; Fungi - 110; Plants - 73; Viruses - 0; Other Eukaryotes - 45 (source: NCBI BLink). )

HSP 1 Score: 126.7 bits (317), Expect = 5.6e-29
Identity = 100/395 (25.32%), Postives = 180/395 (45.57%), Query Frame = 0

Query: 95  FLLSYGVRVGIGILLRAFKLARRQSYSSILDLKQLVSEKDLIVR--EEACRVGLLFGGFT 154
           F +  G++ G+ I     + ARR+  S         S  + I    +E  R GL  G F 
Sbjct: 66  FTIGTGLKGGLAIFSIVARFARRRRSSPQSRKTGEFSNSEAIAMGIKETLRYGLFLGTFA 125

Query: 155 GSY----HALRCLLRKCRKKETSLNVILAGSVAGLSVLALDGSNRRRTLALYLMARLAQC 214
           G++     A+  L     K+      + AG VAG S+L    + +  +LA+Y++ R A  
Sbjct: 126 GTFVSVDEAIAALAGD--KRTAKWRALFAGLVAGPSMLLTGPNTQHTSLAVYILMRAAVL 185

Query: 215 AYNSAKSKNKFHFWGS--HWRHGDSLLFAMACAQVMYSFVMRPESLPKAYRDFIQITGPV 274
           A        +F        W+HGD  L  ++ +Q++ +++++ ESLP +Y+ F+   G  
Sbjct: 186 ASRCGIKSKRFGTICKPLTWKHGDLFLMCLSSSQILSAYILKQESLPSSYKSFLNKQGGK 245

Query: 275 AGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYGNLDEFPSI-IPCSIIHPDTNSCMAH 334
              + + V+D     P         +    KS   ++   P++ +PC+IIH    SC+ H
Sbjct: 246 DLSILQGVKDIATAQPFTNLRA---IEKYYKSVGVDIKLDPTMKVPCTIIH-GNESCVKH 305

Query: 335 NTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAPGRTCWLAVKGAVRSTSFLSAFVGI 394
                   +K+  P+Y  +  +P +++H Q  +          + G  RS+ FL+ +   
Sbjct: 306 GVTFFLQAYKRALPVYVPVYLIPALIVHRQDLLKKQYSILGKGLLGTARSSLFLATYCSS 365

Query: 395 FQAVIC-LHRKVATKDHKLVYWVAGGISALSVLLEKKGRRSELALYVLPRAADSWWYILV 454
             A  C L R   T +  LV  +A   + L++ +EKK RR E++LY L RA +S++  + 
Sbjct: 366 AWAWTCLLFRTFETCNIPLVA-IATFPTGLALAIEKKSRRIEISLYCLARAIESFFTCMT 425

Query: 455 NRHLV---PNIKNAEVFLFSICMGGLMYYLEYEPD 477
               +    +++ A+V +FS+    +M+    E D
Sbjct: 426 EAGYIRPPKSLRRADVVVFSVSTAIIMHCYAQERD 453

BLAST of Carg20825 vs. TAIR 10
Match: AT1G34630.2 (FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: cellular_component unknown; EXPRESSED IN: 25 plant structures; EXPRESSED DURING: 15 growth stages; BEST Arabidopsis thaliana protein match is: Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family protein (TAIR:AT5G51150.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 98.2 bits (243), Expect = 2.1e-20
Identity = 75/307 (24.43%), Postives = 134/307 (43.65%), Query Frame = 0

Query: 95  FLLSYGVRVGIGILLRAFKLARRQSYSSILDLKQLVSEKDLIVR--EEACRVGLLFGGFT 154
           F +  G++ G+ I     + ARR+  S         S  + I    +E  R GL  G F 
Sbjct: 66  FTIGTGLKGGLAIFSIVARFARRRRSSPQSRKTGEFSNSEAIAMGIKETLRYGLFLGTFA 125

Query: 155 GSY----HALRCLLRKCRKKETSLNVILAGSVAGLSVLALDGSNRRRTLALYLMARLAQC 214
           G++     A+  L     K+      + AG VAG S+L    + +  +LA+Y++ R A  
Sbjct: 126 GTFVSVDEAIAALAGD--KRTAKWRALFAGLVAGPSMLLTGPNTQHTSLAVYILMRAAVL 185

Query: 215 AYNSAKSKNKFHFWGS--HWRHGDSLLFAMACAQVMYSFVMRPESLPKAYRDFIQITGPV 274
           A        +F        W+HGD  L  ++ +Q++ +++++ ESLP +Y+ F+   G  
Sbjct: 186 ASRCGIKSKRFGTICKPLTWKHGDLFLMCLSSSQILSAYILKQESLPSSYKSFLNKQGGK 245

Query: 275 AGPVYKAVRDSCRGHPVDGASLSSYLSDRQKSGYGNLDEFPSI-IPCSIIHPDTNSCMAH 334
              + + V+D     P         +    KS   ++   P++ +PC+IIH    SC+ H
Sbjct: 246 DLSILQGVKDIATAQPFTNLRA---IEKYYKSVGVDIKLDPTMKVPCTIIH-GNESCVKH 305

Query: 335 NTQAASATFKKTFPLYFSLTFVPFVVLHLQKFMDAPGRTCWLAVKGAVRSTSFLSAFVGI 393
                   +K+  P+Y  +  +P +++H Q  +          + G  RS+ FL+ +   
Sbjct: 306 GVTFFLQAYKRALPVYVPVYLIPALIVHRQDLLKKQYSILGKGLLGTARSSLFLATYCSS 365

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG6579643.16.3e-309100.00Transmembrane protein 135, partial [Cucurbita argyrosperma subsp. sororia] >KAG7... [more]
XP_022928778.13.3e-30599.26uncharacterized protein LOC111435600 [Cucurbita moschata][more]
XP_023549889.16.2e-30498.71uncharacterized protein LOC111808251 [Cucurbita pepo subsp. pepo][more]
XP_022969748.12.6e-30298.15uncharacterized protein LOC111468853 [Cucurbita maxima] >XP_022969749.1 uncharac... [more]
XP_038874369.17.1e-28492.07uncharacterized protein LOC120067061 [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q86UB92.5e-0530.10Transmembrane protein 135 OS=Homo sapiens OX=9606 GN=TMEM135 PE=2 SV=2[more]
Q3ZBE63.2e-0531.07Transmembrane protein 135 OS=Bos taurus OX=9913 GN=TMEM135 PE=2 SV=1[more]
Q6GQ393.2e-0525.63Transmembrane protein 135 OS=Xenopus laevis OX=8355 GN=tmem135 PE=2 SV=1[more]
Q9CYV51.6e-0429.13Transmembrane protein 135 OS=Mus musculus OX=10090 GN=Tmem135 PE=2 SV=1[more]
Q5U4F42.1e-0429.13Transmembrane protein 135 OS=Rattus norvegicus OX=10116 GN=Tmem135 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EQ311.6e-30599.26uncharacterized protein LOC111435600 OS=Cucurbita moschata OX=3662 GN=LOC1114356... [more]
A0A6J1I3K41.3e-30298.15uncharacterized protein LOC111468853 OS=Cucurbita maxima OX=3661 GN=LOC111468853... [more]
A0A5A7UIM22.1e-28191.14Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
A0A1S3CT922.1e-28191.14uncharacterized protein LOC103504699 OS=Cucumis melo OX=3656 GN=LOC103504699 PE=... [more]
A0A0A0KRJ11.1e-27990.77Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G168790 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT5G51150.16.1e-21772.91Mitochondrial import inner membrane translocase subunit Tim17/Tim22/Tim23 family... [more]
AT1G34630.15.6e-2925.32BEST Arabidopsis thaliana protein match is: Mitochondrial import inner membrane ... [more]
AT1G34630.22.1e-2024.43FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknow... [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 22..56
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..32
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..43
NoneNo IPR availablePANTHERPTHR12459:SF17BNAC03G16050D PROTEINcoord: 20..543
IPR031926Transmembrane protein 135, N-terminal domainPFAMPF15982TMEM135_C_richcoord: 314..439
e-value: 1.2E-6
score: 29.1
IPR026749Transmembrane protein 135PANTHERPTHR12459UNCHARACTERIZEDcoord: 20..543

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20825-RACarg20825-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane