Carg20797 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20797
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionWAT1-related protein
LocationCarg_Chr15: 9446730 .. 9448779 (+)
RNA-Seq ExpressionCarg20797
SyntenyCarg20797
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCAAGGGCGGGAGTGTCAATGAGGAAGCTGTATGCGCCGTGTGCAGCAATGGTGTTGGTGTATATTGCGTATGGCGGATCCCACATTCTGGTGAAGATCGCTAGCGACAAGGGTCTTAACCCTTTGGTCTTCATCGTTTATCGCCATTTCATAGCCTTCCTCGTCTTGGCTCCTTTTGCTTATCTCATCGACAGGTGCGACCAAAATTGTTTGCTTCTTATATCATTTTCTTGGCTTAATGGTTGATTACATGGTGCCTTTGTATAGAAACAAACCAACGTCTCTCACGATTTCGATGACGATAAACATCTTTTTGCTCGCAATCTTAGGCTCAACGATTCACCTGAATCTATTCTACGCTGGAATTAGCTATACTTCGCCTATAGTAACTTCTACTTTCAGCAACATCATTCCAAGCTTGACATTTGTCATGGCTGTTCTATTTCGGTACTGTACCTATTCTTTTTTCTGTATAGATTAATAAAAATATTCATAAATTTTATACTTTTATCTCGAAAGATTGGAGAGAGTGAATATCAAAACTGCGAGAGGTGGTGCTAAAGTGTTGGGGACGATTATGTGCATCGGTGGCTCCTTTGTTTTCACGTTTTGGAAAGGACCATATGTAACCAAAGGCATCTTCAAGGAGCCACTCATAGACGTGTACAAGAATCAAGGCTGTGGACATAGGAGTGGCGAGGATTGGATAAAGGGCTCGGCTTTGATTGTTGTAAGTGAGGTTGCTTGGAGTGCATGGCTAATTTTATTGGTAAGTTCATTCAAAGTCTTCTTTTATGCAAAGAAATGTGTTGCGACACAATCTGCTCATCCAATCTTAATGGCTTGCAGACATTGGTCACTAGAAAATACCCAGCACAATTGACCATTACTGTTTTAATTTGCTTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGATCTTTGCTAGAGATTTAGAAAAATGGAAGCTACACTGGGATGCACGGCTGTTAACCATCGTATATTGTGTAAGTATAGTCGATTGATTTGTTTCTTGTCCTTATATTTATTTATTCTACCCGGACCTATTCCAAATAAGCTAGGAAATTAGTGTTTAAATGGAGAAGGAGAGAAGTATGTGAGAGGTTTCTCCCCACTAGATAAACACAGCTGACGTCTCCGTCCGTGCCCAATTCCCTATTCTACCGGAATCGTTACAACTTTTTTTACATATAACAATAAAAAATATATTTTTCTATTATAAATTATTATAATTTTTTTTTTAATTTGTTTTGTGTTACCATTACATTTCCATGACATTTTACAAATTATATTTTATTTTGGCTAATAAGTCTTAAAGTTTGTGAAACATCAATATTAATAATATTTATACAAGGTAAACTCTTTCCATGTAACAGTTTAAACTCACTGCTAACAGATATTATGTGTTTTTGCATATTAGGTGTCGTCATTACCCTCATAATTTTAAAAGTGCCTACAAGATAGAGGTTTCGACGCGCGTACAAGAAAGGTTTTGTTTTCCTTGTAGGCCAACAATCTCGCTGACACACCGCTCGATGCTTGACTTTGATATAGCTTCTTGATTTGTGTGTGTTGACGTGTTTTAAAATCGTGAGGCTGACAGTACCACGTAACAAACTAAAACGAATAATATATGTTAGCAGTAGACTTGAACTGTTATAGTTTATCACTCTCAAATCCCTTCTTAAATCATCTACTCTCCTATTTGTATATAGGGCGTTGTGATTTCGGGCGCGGGGTATTTCCTACAAACTTGGTGCATCAGCCGCAACGGGCCTGTTTTTACTTCAATGTTTAATCCCCTAATGCTGATATTCGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCACGTGGGCAGGTAAGCCACTCACTTCCTACTATGTTTGTATTGAATTGAAGGTGTTGCCTGAAACTGTTGCGTTATGAAGCCTGGTAGGAGCTTTCCTTATCATACTGGGTCTGTACTTGGTGCTGTGGGGTAAGAAGGCAGATCATGAGGTTGCACCTGAACCGTAG

mRNA sequence

ATGGCAAGGGCGGGAGTGTCAATGAGGAAGCTGTATGCGCCGTGTGCAGCAATGGTGTTGGTGTATATTGCGTATGGCGGATCCCACATTCTGGTGAAGATCGCTAGCGACAAGGGTCTTAACCCTTTGGTCTTCATCGTTTATCGCCATTTCATAGCCTTCCTCGTCTTGGCTCCTTTTGCTTATCTCATCGACAGAAACAAACCAACGTCTCTCACGATTTCGATGACGATAAACATCTTTTTGCTCGCAATCTTAGGCTCAACGATTCACCTGAATCTATTCTACGCTGGAATTAGCTATACTTCGCCTATAGTAACTTCTACTTTCAGCAACATCATTCCAAGCTTGACATTTGTCATGGCTGTTCTATTTCGATTGGAGAGAGTGAATATCAAAACTGCGAGAGGTGGTGCTAAAGTGTTGGGGACGATTATGTGCATCGGTGGCTCCTTTGTTTTCACGTTTTGGAAAGGACCATATGTAACCAAAGGCATCTTCAAGGAGCCACTCATAGACGTGTACAAGAATCAAGGCTGTGGACATAGGAGTGGCGAGGATTGGATAAAGGGCTCGGCTTTGATTGTTGTAAGTGAGGTTGCTTGGAGTGCATGGCTAATTTTATTGACATTGGTCACTAGAAAATACCCAGCACAATTGACCATTACTGTTTTAATTTGCTTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGATCTTTGCTAGAGATTTAGAAAAATGGAAGCTACACTGGGATGCACGGCTGTTAACCATCGTATATTGTGGCGTTGTGATTTCGGGCGCGGGGTATTTCCTACAAACTTGGTGCATCAGCCGCAACGGGCCTGTTTTTACTTCAATGTTTAATCCCCTAATGCTGATATTCGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCACGTGGGCAGCCTGGTAGGAGCTTTCCTTATCATACTGGGTCTGTACTTGGTGCTGTGGGGTAAGAAGGCAGATCATGAGGTTGCACCTGAACCGTAG

Coding sequence (CDS)

ATGGCAAGGGCGGGAGTGTCAATGAGGAAGCTGTATGCGCCGTGTGCAGCAATGGTGTTGGTGTATATTGCGTATGGCGGATCCCACATTCTGGTGAAGATCGCTAGCGACAAGGGTCTTAACCCTTTGGTCTTCATCGTTTATCGCCATTTCATAGCCTTCCTCGTCTTGGCTCCTTTTGCTTATCTCATCGACAGAAACAAACCAACGTCTCTCACGATTTCGATGACGATAAACATCTTTTTGCTCGCAATCTTAGGCTCAACGATTCACCTGAATCTATTCTACGCTGGAATTAGCTATACTTCGCCTATAGTAACTTCTACTTTCAGCAACATCATTCCAAGCTTGACATTTGTCATGGCTGTTCTATTTCGATTGGAGAGAGTGAATATCAAAACTGCGAGAGGTGGTGCTAAAGTGTTGGGGACGATTATGTGCATCGGTGGCTCCTTTGTTTTCACGTTTTGGAAAGGACCATATGTAACCAAAGGCATCTTCAAGGAGCCACTCATAGACGTGTACAAGAATCAAGGCTGTGGACATAGGAGTGGCGAGGATTGGATAAAGGGCTCGGCTTTGATTGTTGTAAGTGAGGTTGCTTGGAGTGCATGGCTAATTTTATTGACATTGGTCACTAGAAAATACCCAGCACAATTGACCATTACTGTTTTAATTTGCTTGTTTGCAACTGCACAATCTGGCTTCCTTGCCTTGATCTTTGCTAGAGATTTAGAAAAATGGAAGCTACACTGGGATGCACGGCTGTTAACCATCGTATATTGTGGCGTTGTGATTTCGGGCGCGGGGTATTTCCTACAAACTTGGTGCATCAGCCGCAACGGGCCTGTTTTTACTTCAATGTTTAATCCCCTAATGCTGATATTCGTGGCCATATTCTCAGCATTTGTGTTCTCAGAGCGGCTTCACGTGGGCAGCCTGGTAGGAGCTTTCCTTATCATACTGGGTCTGTACTTGGTGCTGTGGGGTAAGAAGGCAGATCATGAGGTTGCACCTGAACCGTAG

Protein sequence

MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
Homology
BLAST of Carg20797 vs. NCBI nr
Match: KAG7017071.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.1 bits (1725), Expect = 2.1e-188
Identity = 341/341 (100.00%), Postives = 341/341 (100.00%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV
Sbjct: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC
Sbjct: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI
Sbjct: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 341

BLAST of Carg20797 vs. NCBI nr
Match: KAG6579614.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 666.0 bits (1717), Expect = 1.7e-187
Identity = 339/341 (99.41%), Postives = 340/341 (99.71%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV
Sbjct: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MAVLFRLERVNI+TARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC
Sbjct: 121 MAVLFRLERVNIRTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI
Sbjct: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKLHWDARLLTIVYCGVVISG GYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLHWDARLLTIVYCGVVISGLGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 341

BLAST of Carg20797 vs. NCBI nr
Match: XP_022928963.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 659.1 bits (1699), Expect = 2.1e-185
Identity = 335/341 (98.24%), Postives = 338/341 (99.12%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MARAGVSMRKLY PCAAMVLVYIAYGGSHILVKIAS+KGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MARAGVSMRKLYGPCAAMVLVYIAYGGSHILVKIASNKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTF+NIIPSLTFV
Sbjct: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFNNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MA LFRLERVNI+TARGGAKVLGTI CIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC
Sbjct: 121 MAFLFRLERVNIRTARGGAKVLGTITCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI
Sbjct: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 341

BLAST of Carg20797 vs. NCBI nr
Match: XP_023520568.1 (WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo] >XP_023521475.1 WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 639.0 bits (1647), Expect = 2.3e-179
Identity = 326/341 (95.60%), Postives = 332/341 (97.36%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MA AGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MATAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNK TSLT+SMTINIFLLAILGSTIHLNLFYAGISYTSPIVT+TFSNIIPSLTFV
Sbjct: 61  AYLIDRNKRTSLTLSMTINIFLLAILGSTIHLNLFYAGISYTSPIVTTTFSNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MAVLFRLERVNIKTARGGAKVLGTI+CIGGSFVFTFWKGPYVTKG+FKEPLIDVYKNQG 
Sbjct: 121 MAVLFRLERVNIKTARGGAKVLGTIVCIGGSFVFTFWKGPYVTKGMFKEPLIDVYKNQGS 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GH SGEDWIKGS LI+VSEVAWSAWLILL LVTRKYPAQLTITVL+CLFATAQSGFLALI
Sbjct: 181 GHSSGEDWIKGSGLILVSEVAWSAWLILLALVTRKYPAQLTITVLMCLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKL WDARLLTIVYCGVVISG GYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLQWDARLLTIVYCGVVISGVGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVA EP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAAEP 341

BLAST of Carg20797 vs. NCBI nr
Match: XP_022928958.1 (WAT1-related protein At1g43650-like [Cucurbita moschata])

HSP 1 Score: 552.0 bits (1421), Expect = 3.7e-153
Identity = 275/334 (82.34%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV+V I YGGS+IL+KIAS+K LNP VF+VYRHFIAFL+LAPFAY+IDRN
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           KP SLTIS  I IFLLAILGSTIHLNLFY GI YTSP V S FSN+IPSLTFVMAVLFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNI+TARG AKV+GTI+CIGGS +FT WKGPYVTK IFKEPLID+YKN   G+ SG+D
Sbjct: 121 EKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA I++S++AWS WLIL   VTRKYPAQLT+TVLICLFATAQSGFLALIFARD EK
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G+VISG GYFLQTWCISRNGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA EP
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEP 334

BLAST of Carg20797 vs. ExPASy Swiss-Prot
Match: Q6NMB7 (WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2 SV=1)

HSP 1 Score: 239.2 bits (609), Expect = 6.9e-62
Identity = 126/324 (38.89%), Postives = 198/324 (61.11%), Query Frame = 0

Query: 17  AMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISM 76
           AMV V I Y G  +L K+A  +G NP VF+ YR   A L L+PFA+ ++ +K + L+  +
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 77  TINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTAR 136
            + IF +++ G T+ LNL+Y  I  T+    +  +N IPS+TFV+A+LFRLE V +K + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 137 GGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGH---RSGEDWIKGSA 196
           G AKV G+++ + G+ VF F KGP          LI+ Y +    +    S ++ +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 197 LIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWD 256
            ++ +   W  W+I+ + V ++YPA+L +  L CLF+  QS   A+   R+   WK+ + 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 257 ARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGS 316
             LL++ YCG++++G  Y+LQ W I + GPVFT+++ PL LI   I S+F+F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

Query: 317 LVGAFLIILGLYLVLWGKKADHEV 338
           + GA L++ GLYL LWGK  + E+
Sbjct: 310 VGGAVLLVCGLYLGLWGKTKEEEI 324

BLAST of Carg20797 vs. ExPASy Swiss-Prot
Match: Q9FL41 (WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2 SV=1)

HSP 1 Score: 234.6 bits (597), Expect = 1.7e-60
Identity = 115/329 (34.95%), Postives = 199/329 (60.49%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P  AM+ +   Y G +I+ KI+ + G++  V +VYRH IA  V+APFA+  +R     +T
Sbjct: 18  PYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKIT 77

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
            S+ + +F+L +LG  I  N +Y G+ YTSP  +   SN++P++TF++AVLFR+E +++K
Sbjct: 78  FSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLK 137

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYV----TKGIFKEPLIDVYKNQGCGHRSGEDWI 193
                AK+ GT++ + G+ + T +KGP V    TK +  +              S ++++
Sbjct: 138 KLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFL 197

Query: 194 KGSALIVVSEVAWSAWLILLTLVTRKYPA-QLTITVLICLFATAQSGFLALIFARDLEKW 253
           KGS L++ + +AW++  +L   + + Y   QL++T LIC   T Q+  +  +   +   W
Sbjct: 198 KGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAW 257

Query: 254 KLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSER 313
           ++ WD  LL   Y G+V S   Y++Q   + + GPVF + F+PLM++ VA+  +FV +E+
Sbjct: 258 RIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEK 317

Query: 314 LHVGSLVGAFLIILGLYLVLWGKKADHEV 338
           + +G ++GA LI++GLY VLWGK+ +++V
Sbjct: 318 IFLGGVIGAVLIVIGLYAVLWGKQKENQV 346

BLAST of Carg20797 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 233.8 bits (595), Expect = 2.9e-60
Identity = 119/332 (35.84%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P   + ++ + Y    ++ K   + G+N  VF+ YR   A + LAP A+  +R     L+
Sbjct: 8   PYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPLS 67

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
               I IF+L++ G T+ L+L    +SYTS  + +  +  +P++TF +A+LF +ER+ +K
Sbjct: 68  FVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKVK 127

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLI-DVYKNQGCGHRS-------- 193
           + +G AK++G  +C+GG  +   +KGP     + K PL    Y  Q   HR+        
Sbjct: 128 SIQGTAKLVGITVCMGGVIILAIYKGP-----LLKLPLCPHFYHGQEHPHRNNPGHVSGG 187

Query: 194 GEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARD 253
              W+KG  L++ S + W  WL+L   V + YP++L  T L CL ++ QS  +A+   RD
Sbjct: 188 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 247

Query: 254 LEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFV 313
           +  WKL W+ RL+ ++YCG +++G  Y+LQ+W I + GPVF SMF PL L+F  + SA +
Sbjct: 248 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 307

Query: 314 FSERLHVGSLVGAFLIILGLYLVLWGKKADHE 337
             E + +GS+VG  L+I+GLY VLWGK  + +
Sbjct: 308 LCEIISLGSIVGGLLLIIGLYCVLWGKSREEK 334

BLAST of Carg20797 vs. ExPASy Swiss-Prot
Match: Q6J163 (Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1)

HSP 1 Score: 231.5 bits (589), Expect = 1.4e-59
Identity = 124/340 (36.47%), Postives = 195/340 (57.35%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           M R  V M +     AAM+ +   Y G HI+ + A + G++ +VF VYR+ +A +++ P 
Sbjct: 6   MQRCNVFMSERVKLHAAMLALQFGYAGFHIVSRAALNMGVSKVVFPVYRNILALMLIGPC 65

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AY +++ +  +LT+S  I  FLLA+ G T    +    I    P   S   N +P++TF+
Sbjct: 66  AYFLEKKERPALTLSFLIQFFLLALCGITGQSRILSLRIVLHIPTFASAIQNSVPAITFI 125

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPL------IDV 180
           MA   RLE+V+I    G AK++GT+ C+ G+ + T +KGP +T  I++  L         
Sbjct: 126 MAAALRLEKVHISRRDGLAKIIGTVACVSGATIITLYKGPPITH-IWRPNLEVTASYFKA 185

Query: 181 YKNQGCGHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQS 240
           ++      +S E+W  G   ++ + +AWS W++L   V ++YPA+L++T   C F   Q 
Sbjct: 186 FQGNDLSAKS-ENWTLGCIYLLGNCLAWSGWIVLQAPVLKRYPARLSVTSFTCFFGVIQF 245

Query: 241 GFLALIFARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLML 300
             +A  F  DLE WK+H    L TI+Y G V SG  + +Q WCI R GPVF +++ P+  
Sbjct: 246 LIIAAFFETDLEHWKIHSGGELFTILYAGFVASGIAFSVQIWCIDRGGPVFVAVYQPVQT 305

Query: 301 IFVAIFSAFVFSERLHVGSLVGAFLIILGLYLVLWGKKAD 335
           I VAI ++ +  E+ ++G + GA LII+GLYLVLWGK  +
Sbjct: 306 IAVAIMASIILGEQFYLGGIFGAILIIIGLYLVLWGKSEE 343

BLAST of Carg20797 vs. ExPASy Swiss-Prot
Match: O80638 (WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2 SV=1)

HSP 1 Score: 231.1 bits (588), Expect = 1.9e-59
Identity = 121/328 (36.89%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 10  KLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKP 69
           K + P   +V +   Y G  I+ K A ++G++P V   YRH +A + +APFAY +DR   
Sbjct: 4   KTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIR 63

Query: 70  TSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLER 129
             +T+S+   I LL +L  TI  NL+Y G+ YTS   T+  +N++P+  F+MA +FRLE+
Sbjct: 64  PKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEK 123

Query: 130 VNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSG--ED 189
           VN+K     AK+LGTI+ +GG+ + T  KGP +       P  + +        +G  +D
Sbjct: 124 VNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPL-----PWANPHDIHQDSSNTGVKQD 183

Query: 190 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFAR-DLE 249
             KG++LI +  + W+ ++ L  +  + YP +L++T  IC   + +S  +AL   R +  
Sbjct: 184 LTKGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPS 243

Query: 250 KWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFS 309
            W +H D++LL  VY GV+ SG GY++Q   +   GPVF + FNPL ++ VAI  + + +
Sbjct: 244 AWAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILA 303

Query: 310 ERLHVGSLVGAFLIILGLYLVLWGKKAD 335
           E + +G ++GA +I+LGLY VLWGK  D
Sbjct: 304 EVMFLGRILGAIVIVLGLYSVLWGKSKD 326

BLAST of Carg20797 vs. ExPASy TrEMBL
Match: A0A6J1EQM5 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.0e-185
Identity = 335/341 (98.24%), Postives = 338/341 (99.12%), Query Frame = 0

Query: 1   MARAGVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPF 60
           MARAGVSMRKLY PCAAMVLVYIAYGGSHILVKIAS+KGLNPLVFIVYRHFIAFLVLAPF
Sbjct: 1   MARAGVSMRKLYGPCAAMVLVYIAYGGSHILVKIASNKGLNPLVFIVYRHFIAFLVLAPF 60

Query: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFV 120
           AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTF+NIIPSLTFV
Sbjct: 61  AYLIDRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFNNIIPSLTFV 120

Query: 121 MAVLFRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180
           MA LFRLERVNI+TARGGAKVLGTI CIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC
Sbjct: 121 MAFLFRLERVNIRTARGGAKVLGTITCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGC 180

Query: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240
           GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI
Sbjct: 181 GHRSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALI 240

Query: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300
           FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF
Sbjct: 241 FARDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIF 300

Query: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP
Sbjct: 301 SAFVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 341

BLAST of Carg20797 vs. ExPASy TrEMBL
Match: A0A6J1EQL7 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1)

HSP 1 Score: 552.0 bits (1421), Expect = 1.8e-153
Identity = 275/334 (82.34%), Postives = 299/334 (89.52%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV+V I YGGS+IL+KIAS+K LNP VF+VYRHFIAFL+LAPFAY+IDRN
Sbjct: 1   MAKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           KP SLTIS  I IFLLAILGSTIHLNLFY GI YTSP V S FSN+IPSLTFVMAVLFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNI+TARG AKV+GTI+CIGGS +FT WKGPYVTK IFKEPLID+YKN   G+ SG+D
Sbjct: 121 EKVNIRTARGAAKVVGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYKNHDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA I++S++AWS WLIL   VTRKYPAQLT+TVLICLFATAQSGFLALIFARD EK
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G+VISG GYFLQTWCISRNGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA EP
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEP 334

BLAST of Carg20797 vs. ExPASy TrEMBL
Match: A0A6J1I2R9 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1)

HSP 1 Score: 550.1 bits (1416), Expect = 6.7e-153
Identity = 274/334 (82.04%), Postives = 298/334 (89.22%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV+V I YGGS+IL+KIAS+K LNP VF+VYRHFIAFL+LAPFAY+IDRN
Sbjct: 1   MTKLYASCAAMVVVQIGYGGSNILMKIASEKDLNPFVFVVYRHFIAFLLLAPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           KP SLTIS  I IFLLAILGSTIHLNLFY GI YTSP V S FSN+IPSLTFVMAVLFRL
Sbjct: 61  KPASLTISTAIKIFLLAILGSTIHLNLFYVGIGYTSPTVASAFSNVIPSLTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNI+TARG AKV GTI+CIGGS +FT WKGPYVTK IFKEPLID+Y+N   G+ SG+D
Sbjct: 121 EKVNIRTARGAAKVAGTILCIGGSLIFTLWKGPYVTKAIFKEPLIDIYRNHDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA I++S++AWS WLIL   VTRKYPAQLT+TVLICLFATAQSGFLALIFARD EK
Sbjct: 181 WIKGSAFILISDIAWSIWLILQAFVTRKYPAQLTMTVLICLFATAQSGFLALIFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G+VISG GYFLQTWCISRNGPVFTSMF+PLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIVISGVGYFLQTWCISRNGPVFTSMFSPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPEP 342
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA EP
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVAAEP 334

BLAST of Carg20797 vs. ExPASy TrEMBL
Match: A0A6J1HY24 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1)

HSP 1 Score: 526.2 bits (1354), Expect = 1.0e-145
Identity = 264/333 (79.28%), Postives = 291/333 (87.39%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV V I YGGS+IL+K+AS+K L+P VF VYRH IAFLVL PFAY+IDRN
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKVASEKDLDPFVFFVYRHIIAFLVLLPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           K TSLTIS TI IFLLAILGST +LNLFY GI YTS  V S FSN+ PS TFVMAVLFRL
Sbjct: 61  KQTSLTISTTIKIFLLAILGSTTYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNIKTARG AKVLGT++CIGGS +FTFWKGPY+TK IFKEPLI++YKNQ  G+ SG+D
Sbjct: 121 EKVNIKTARGAAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA IV+SE++WSAWLIL T VTRKYPA+LTITVL C+FATAQSGFLAL FARD EK
Sbjct: 181 WIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKLHWDARLLTIVY G++IS  GYF+QTW IS NGPVFTS+FNPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLHWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPE 341
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA E
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVADE 333

BLAST of Carg20797 vs. ExPASy TrEMBL
Match: A0A6J1ESW6 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1)

HSP 1 Score: 525.4 bits (1352), Expect = 1.8e-145
Identity = 264/333 (79.28%), Postives = 291/333 (87.39%), Query Frame = 0

Query: 8   MRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRN 67
           M KLYA CAAMV V I YGGS+IL+K+AS+K L+P VF VYRH IAFLVL PFAY+IDRN
Sbjct: 1   MAKLYASCAAMVAVQIGYGGSNILMKVASEKDLDPFVFFVYRHIIAFLVLLPFAYVIDRN 60

Query: 68  KPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRL 127
           K  SLTISMTI IFLLAILGST +LNLFY GI YTS  V S FSN+ PS TFVMAVLFRL
Sbjct: 61  KRASLTISMTIKIFLLAILGSTTYLNLFYLGIKYTSATVASAFSNVTPSFTFVMAVLFRL 120

Query: 128 ERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSGED 187
           E+VNIKTARGGAKVLGT++CIGGS +FTFWKGPY+TK IFKEPLI++YKNQ  G+ SG+D
Sbjct: 121 EKVNIKTARGGAKVLGTVICIGGSLIFTFWKGPYLTKAIFKEPLINIYKNQDSGNSSGKD 180

Query: 188 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEK 247
           WIKGSA IV+SE++WSAWLIL T VTRKYPA+LTITVL C+FATAQSGFLAL FARD EK
Sbjct: 181 WIKGSAFIVLSEMSWSAWLILQTFVTRKYPAKLTITVLTCVFATAQSGFLALFFARDPEK 240

Query: 248 WKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSE 307
           WKL WDARLLTIVY G++IS  GYF+QTW IS NGPVFTS+FNPLMLIFVAIFSAFVFSE
Sbjct: 241 WKLQWDARLLTIVYSGIIISAVGYFVQTWVISHNGPVFTSIFNPLMLIFVAIFSAFVFSE 300

Query: 308 RLHVGSLVGAFLIILGLYLVLWGKKADHEVAPE 341
           RLHVGSLVGAF I+LGLYLVLWGKKADH VA E
Sbjct: 301 RLHVGSLVGAFFIVLGLYLVLWGKKADHVVADE 333

BLAST of Carg20797 vs. TAIR 10
Match: AT1G43650.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 239.2 bits (609), Expect = 4.9e-63
Identity = 126/324 (38.89%), Postives = 198/324 (61.11%), Query Frame = 0

Query: 17  AMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLTISM 76
           AMV V I Y G  +L K+A  +G NP VF+ YR   A L L+PFA+ ++ +K + L+  +
Sbjct: 10  AMVFVQIVYAGMPLLSKVAISQGTNPFVFVFYRQAFAALALSPFAFFLESSKSSPLSFIL 69

Query: 77  TINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIKTAR 136
            + IF +++ G T+ LNL+Y  I  T+    +  +N IPS+TFV+A+LFRLE V +K + 
Sbjct: 70  LLKIFFISLCGLTLSLNLYYVAIENTTATFAAATTNAIPSITFVLALLFRLETVTLKKSH 129

Query: 137 GGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGH---RSGEDWIKGSA 196
           G AKV G+++ + G+ VF F KGP          LI+ Y +    +    S ++ +KGS 
Sbjct: 130 GVAKVTGSMVGMLGALVFAFVKGP---------SLINHYNSSTIPNGTVPSTKNSVKGSI 189

Query: 197 LIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARDLEKWKLHWD 256
            ++ +   W  W+I+ + V ++YPA+L +  L CLF+  QS   A+   R+   WK+ + 
Sbjct: 190 TMLAANTCWCLWIIMQSKVMKEYPAKLRLVALQCLFSCIQSAVWAVAVNRNPSVWKIEFG 249

Query: 257 ARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSERLHVGS 316
             LL++ YCG++++G  Y+LQ W I + GPVFT+++ PL LI   I S+F+F E  ++GS
Sbjct: 250 LPLLSMAYCGIMVTGLTYWLQVWAIEKKGPVFTALYTPLALILTCIVSSFLFKETFYLGS 309

Query: 317 LVGAFLIILGLYLVLWGKKADHEV 338
           + GA L++ GLYL LWGK  + E+
Sbjct: 310 VGGAVLLVCGLYLGLWGKTKEEEI 324

BLAST of Carg20797 vs. TAIR 10
Match: AT5G07050.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 234.6 bits (597), Expect = 1.2e-61
Identity = 115/329 (34.95%), Postives = 199/329 (60.49%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P  AM+ +   Y G +I+ KI+ + G++  V +VYRH IA  V+APFA+  +R     +T
Sbjct: 18  PYFAMISLQFGYAGMNIITKISLNTGMSHYVLVVYRHAIATAVIAPFAFFFERKAQPKIT 77

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
            S+ + +F+L +LG  I  N +Y G+ YTSP  +   SN++P++TF++AVLFR+E +++K
Sbjct: 78  FSIFMQLFILGLLGPVIDQNFYYMGLKYTSPTFSCAMSNMLPAMTFILAVLFRMEMLDLK 137

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYV----TKGIFKEPLIDVYKNQGCGHRSGEDWI 193
                AK+ GT++ + G+ + T +KGP V    TK +  +              S ++++
Sbjct: 138 KLWCQAKIAGTVVTVAGAMLMTIYKGPIVELFWTKYMHIQDSSHANTTSSKNSSSDKEFL 197

Query: 194 KGSALIVVSEVAWSAWLILLTLVTRKYPA-QLTITVLICLFATAQSGFLALIFARDLEKW 253
           KGS L++ + +AW++  +L   + + Y   QL++T LIC   T Q+  +  +   +   W
Sbjct: 198 KGSILLIFATLAWASLFVLQAKILKTYAKHQLSLTTLICFIGTLQAVAVTFVMEHNPSAW 257

Query: 254 KLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFSER 313
           ++ WD  LL   Y G+V S   Y++Q   + + GPVF + F+PLM++ VA+  +FV +E+
Sbjct: 258 RIGWDMNLLAAAYSGIVASSISYYVQGIVMKKRGPVFATAFSPLMMVIVAVMGSFVLAEK 317

Query: 314 LHVGSLVGAFLIILGLYLVLWGKKADHEV 338
           + +G ++GA LI++GLY VLWGK+ +++V
Sbjct: 318 IFLGGVIGAVLIVIGLYAVLWGKQKENQV 346

BLAST of Carg20797 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 233.8 bits (595), Expect = 2.1e-61
Identity = 119/332 (35.84%), Postives = 192/332 (57.83%), Query Frame = 0

Query: 14  PCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKPTSLT 73
           P   + ++ + Y    ++ K   + G+N  VF+ YR   A + LAP A+  +R     L+
Sbjct: 8   PYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPLS 67

Query: 74  ISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLERVNIK 133
               I IF+L++ G T+ L+L    +SYTS  + +  +  +P++TF +A+LF +ER+ +K
Sbjct: 68  FVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKVK 127

Query: 134 TARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLI-DVYKNQGCGHRS-------- 193
           + +G AK++G  +C+GG  +   +KGP     + K PL    Y  Q   HR+        
Sbjct: 128 SIQGTAKLVGITVCMGGVIILAIYKGP-----LLKLPLCPHFYHGQEHPHRNNPGHVSGG 187

Query: 194 GEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFARD 253
              W+KG  L++ S + W  WL+L   V + YP++L  T L CL ++ QS  +A+   RD
Sbjct: 188 STSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIALERD 247

Query: 254 LEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFV 313
           +  WKL W+ RL+ ++YCG +++G  Y+LQ+W I + GPVF SMF PL L+F  + SA +
Sbjct: 248 ISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSSAIL 307

Query: 314 FSERLHVGSLVGAFLIILGLYLVLWGKKADHE 337
             E + +GS+VG  L+I+GLY VLWGK  + +
Sbjct: 308 LCEIISLGSIVGGLLLIIGLYCVLWGKSREEK 334

BLAST of Carg20797 vs. TAIR 10
Match: AT2G39510.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 231.1 bits (588), Expect = 1.3e-60
Identity = 121/328 (36.89%), Postives = 193/328 (58.84%), Query Frame = 0

Query: 10  KLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLIDRNKP 69
           K + P   +V +   Y G  I+ K A ++G++P V   YRH +A + +APFAY +DR   
Sbjct: 4   KTWKPFITVVSLQFGYAGLSIIAKFALNQGMSPHVLASYRHIVATIFIAPFAYFLDRKIR 63

Query: 70  TSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVLFRLER 129
             +T+S+   I LL +L  TI  NL+Y G+ YTS   T+  +N++P+  F+MA +FRLE+
Sbjct: 64  PKMTLSIFFKILLLGLLEPTIDQNLYYTGMKYTSATFTAAMTNVLPAFAFIMAWIFRLEK 123

Query: 130 VNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQGCGHRSG--ED 189
           VN+K     AK+LGTI+ +GG+ + T  KGP +       P  + +        +G  +D
Sbjct: 124 VNVKKIHSQAKILGTIVTVGGAMLMTVVKGPLIPL-----PWANPHDIHQDSSNTGVKQD 183

Query: 190 WIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFAR-DLE 249
             KG++LI +  + W+ ++ L  +  + YP +L++T  IC   + +S  +AL   R +  
Sbjct: 184 LTKGASLIAIGCICWAGFINLQAITLKSYPVELSLTAYICFLGSIESTIVALFIERGNPS 243

Query: 250 KWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSAFVFS 309
            W +H D++LL  VY GV+ SG GY++Q   +   GPVF + FNPL ++ VAI  + + +
Sbjct: 244 AWAIHLDSKLLAAVYGGVICSGIGYYVQGVIMKTRGPVFVTAFNPLSMVIVAILGSIILA 303

Query: 310 ERLHVGSLVGAFLIILGLYLVLWGKKAD 335
           E + +G ++GA +I+LGLY VLWGK  D
Sbjct: 304 EVMFLGRILGAIVIVLGLYSVLWGKSKD 326

BLAST of Carg20797 vs. TAIR 10
Match: AT4G08300.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 230.7 bits (587), Expect = 1.7e-60
Identity = 119/338 (35.21%), Postives = 194/338 (57.40%), Query Frame = 0

Query: 5   GVSMRKLYAPCAAMVLVYIAYGGSHILVKIASDKGLNPLVFIVYRHFIAFLVLAPFAYLI 64
           G  M KL  P  A++ +   Y G +I+  ++   G+N  +   YRH +A +V+APFA ++
Sbjct: 3   GGKMDKL-KPIIAIISLQFGYAGMYIITMVSFKHGMNHWILATYRHVVATIVIAPFALIL 62

Query: 65  DRNKPTSLTISMTINIFLLAILGSTIHLNLFYAGISYTSPIVTSTFSNIIPSLTFVMAVL 124
           +R     +T  + + I  L  L   +  NL+Y G+  TS   +S F N +P++TF+MAV+
Sbjct: 63  ERKIRPKMTWPLFLRILALGFLEPLLDQNLYYIGMKATSATYSSAFVNALPAITFIMAVI 122

Query: 125 FRLERVNIKTARGGAKVLGTIMCIGGSFVFTFWKGPYVTKGIFKEPLIDVYKNQG--CGH 184
           FR+E VN+K  R  AKV+GT + +GG+ V T +KGP +   +FK     ++         
Sbjct: 123 FRIETVNLKKTRSLAKVIGTAITVGGAMVMTLYKGPAIE--LFKTAHSSLHGGSSGTSSE 182

Query: 185 RSGEDWIKGSALIVVSEVAWSAWLILLTLVTRKYPAQLTITVLICLFATAQSGFLALIFA 244
            + ++W+ G+  ++ S   W+ + IL +   +KYPA+L++ + IC   T  +   +LI  
Sbjct: 183 TTDQNWVTGTLAVMGSITTWAGFFILQSFTLKKYPAELSLVMWICAMGTVLNTIASLIMV 242

Query: 245 RDLEKWKLHWDARLLTIVYCGVVISGAGYFLQTWCISRNGPVFTSMFNPLMLIFVAIFSA 304
           RD+  WK+  D+  L  VY GVV SG  Y++Q+  I   GPVFT+ F+P+ +I  A    
Sbjct: 243 RDVSAWKVGMDSGTLAAVYSGVVCSGMAYYIQSIVIRERGPVFTTSFSPMCMIITAFLGV 302

Query: 305 FVFSERLHVGSLVGAFLIILGLYLVLWGKKADHEVAPE 341
            V +E++H+GS++GA  I+ GLY V+WGK  D  ++ E
Sbjct: 303 LVLAEKIHLGSIIGAIFIVFGLYSVVWGKAKDEVISVE 337

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7017071.12.1e-188100.00WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6579614.11.7e-18799.41WAT1-related protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022928963.12.1e-18598.24WAT1-related protein At1g43650-like [Cucurbita moschata][more]
XP_023520568.12.3e-17995.60WAT1-related protein At1g43650-like [Cucurbita pepo subsp. pepo] >XP_023521475.1... [more]
XP_022928958.13.7e-15382.34WAT1-related protein At1g43650-like [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q6NMB76.9e-6238.89WAT1-related protein At1g43650 OS=Arabidopsis thaliana OX=3702 GN=At1g43650 PE=2... [more]
Q9FL411.7e-6034.95WAT1-related protein At5g07050 OS=Arabidopsis thaliana OX=3702 GN=At5g07050 PE=2... [more]
Q9FGG32.9e-6035.84WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2... [more]
Q6J1631.4e-5936.47Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1[more]
O806381.9e-5936.89WAT1-related protein At2g39510 OS=Arabidopsis thaliana OX=3702 GN=At2g39510 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1EQM51.0e-18598.24WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435707 PE=3 SV=1[more]
A0A6J1EQL71.8e-15382.34WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435703 PE=3 SV=1[more]
A0A6J1I2R96.7e-15382.04WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469064 PE=3 SV=1[more]
A0A6J1HY241.0e-14579.28WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111469060 PE=3 SV=1[more]
A0A6J1ESW61.8e-14579.28WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435701 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G43650.14.9e-6338.89nodulin MtN21 /EamA-like transporter family protein [more]
AT5G07050.11.2e-6134.95nodulin MtN21 /EamA-like transporter family protein [more]
AT5G64700.12.1e-6135.84nodulin MtN21 /EamA-like transporter family protein [more]
AT2G39510.11.3e-6036.89nodulin MtN21 /EamA-like transporter family protein [more]
AT4G08300.11.7e-6035.21nodulin MtN21 /EamA-like transporter family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000620EamA domainPFAMPF00892EamAcoord: 17..149
e-value: 7.2E-13
score: 48.9
coord: 190..328
e-value: 8.2E-15
score: 55.2
IPR030184WAT1-related proteinPANTHERPTHR31218WAT1-RELATED PROTEINcoord: 14..336
NoneNo IPR availablePANTHERPTHR31218:SF265WAT1-RELATED PROTEINcoord: 14..336
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 234..332
NoneNo IPR availableSUPERFAMILY103481Multidrug resistance efflux transporter EmrEcoord: 48..147

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20797-RACarg20797-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0022857 transmembrane transporter activity