Carg20794 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20794
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Asparagine--tRNA ligase
Location	Carg_Chr10: 5911571 .. 5914721 (-)
RNA-Seq Expression	Carg20794
Synteny	Carg20794

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGATGTCAACCTGTACGGAGACTTTAAATGTCGAGTGTGGATTAGGGTTTAGGATTTCACTTCAGTAGTTGAGGTATCCACCGGCGACAGTCCAATGGCAGAGCATTCCGCGCCGCCCGCTGACCAACTCGCCGCCGTCACCTTGAACGACGATGCTCCCACTTACAACTCTCTCAAAGCCGAATTCTCTGACCGCGTTCCGATCCGTTCAATTGTCTCCCGGCCCGATGGCGGCGCAGGCCTTGCCGGCCAGAAGGTTCGCGTTGGTGGCTGGGTCAAAACCGGAAGAAAAGCCGACAAGGATGCGTTTGCCTTTCTCGAGTTGAACGACGGCTCTTGCCCGCAGAATCTCCAAGTGATCATCGAAGCGTCTTTGGCGGATCTTGGCCAGATTGTCCCTACTGGAACCTGCGTTGTTGTCGATGGCGTCTTCAAGCTTCCTCCACCGGGTAAGCACCAGAAAGTCGAGCTTCGGGCTGATGCTGTCGTTCATGTCGGTCCGGTTGATCCCGCTCGGTATCCTTTACCGAAGACGAAACTCACTCTCGAGTTTCTAAGAGATTTCGTCCATCTCCGCCCTAGAACCAACACGGTAAAAGAAATTGAAATTGTCTTCCGAATTTGAATAATTAGAAATTGAATCAGATTATTTCGATTGGGGTTTTCACATTCGTGGATTTCATTTATTACATTTTTCTGCAACAACACAAATCCACGAGTTCATGTTTTCTCTACGATTACAGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACACACACATTTTTTAACAAGCATGGATTTCTTTATATTCACACACCAATTGTCACCACAAGCGATTGTGAGGGTGCTGGTGAAATGTTTCAAGTTACGACATTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGGTTAAGAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGCAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAAGCTGAAGGCATCTAAAGCTAGCAAGGAGGAAATTGGTGCTTCTGTTATGGAACTGAAGAAGGCAAAGGAGAATGTTTCTAAGCTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCGGAGAAAGATGGGAAAGTTGATTATGCAAAAGACTTCTTTGCTCGACAAGCGTTTTTGACTGTTTCGGGCCAGCTGCAAGTCGAAACATATGCCTGTGCTCTTAGTAGTGTATACACCTTTGGACCAACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGTTTATTTACTATGCTTAAATTTTGTTTCTAATTGTATTCTTTTCTTAGTACTCAATTGGAATTGGTTTCATGTCTCTGGCTTAGAAGATTGACTACGTATAACTATCAAAAGACACAATACCTCTGACATTTTATCTTGTAGTGGATGCTTTATGTTCTAGGATCAGTTTATCTCCTCTTTTAGTTTTGTAATTAATTTCTATAAGAAAGCCATTTTGGTTGGCCTAGTGGTCATTCGTTAATAATAACGATCGGAGGAAATGGGTTCAAGCCATAGTTGCCATTTATCTAAAATTTAACATCTTACGAGTTTCTTTGGTAACTAAATATTAATCTTGTGCACAATCTGGCCTTTCCTTAAGAACTCTAATTGAGAAGAAGCTGCTAGAGGGCTTGGTTTAAGTTCGCCGTGTTCTCTGTACTTTTATGCAATATGGCAATCAACCATGGAACTTGGTTAGGAAATTCTATTAGGAATAAGAAAAACAACGGAACTCTGCAAATAGGGGTCTAGTTCTCTTTTGTTGATTGTATGCTCTTCTTCTAGAGTTTTAATTGAATTAACCTTATGTGACATCTTTTCTATAATTGTTTGAAGGACGATATGAATTGTGCGGAGTCCTACGTGAGATTTTTGTGTCAATGGTTACTTGATAACTGCCTAGATGACATGCAATTTATGGCCGACAAGTACGACCGAACTTGTATTGATCGTCTCAAACTGGTCTCTTCAACCCCTTTTGTTCGAACTTCCTATACGGAAGCTGTTGAAATACTTGAAGAAGCTGTAAAGAAGGGTAAGGTGTTTGAGAACAAAGTGGAATGGGGGATTGATTTAGCTTCAGAGCATGAAAGGTTTGTGAACTCCGATGCTTTTCATTTACATTCCACTTGAAACAATGGTGATTATTTTTCTACTTCCTTGCTCAGATACTTGACTGAGGTAGTGTTTAAAAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCAAAAACTGTAGCTGCAATGGATGTTCTTGTACCAAAGGTAGTTAAGTTATCTGTGATTTGTTTCATCATTTTTGACCTTTTGTTTGGAAAAGCATTGAATTCTTGGCTCTATTGAAGGTTGGGGAGTTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGTGACAGGTAGAAGAACAATACATAGTGTTCCTATTGTTTCCCCTTAGAGTGAACTTTGAAGGCTGAATTCTTTTTCCCTGCAGGATCAAGGAGATAGATTTGCCACTTGAGCCATACGAATGGTATCTCGATCTGAGACGATATGGGACAGTGAAGCACAGCGGTTTTGGCCTCGGCTTCGAGCGAATGCTTCTTTTTGCCACTGGCATTGACAACATCAGAGATGTTATACCCTTCCCCAGATACCCAGGACGAGCAGACCTATGATCATATATATTTACACTAAAGAGAGATGTTGTTGGGTTTTAATTTATGCTCATAAATTTTAGATTGGTGGGTTTATTTTATACAGAAACTATTGCATTTCAAATGACTTAAATTGGTTTGATGTGTTAGATATTTTGGTACTAACAGATTTTAATTTGTTTACAGAGAAGATTGTAAAAAGAATGTAAAAGTGGATCAATGTCACAACAAGAGTCTGTTATGGGAGCGATTACTTCTACATTCATTTCACGTAAGACCCTAATTTTGCTTATGAATTTTTCGTTGAACTTTACTCGAATTTATTCCTTTTAGATTCATTCACTTTTAGCATTTTAAGGATTAAAATGAATATTTTT

mRNA sequence

AGATGTCAACCTGTACGGAGACTTTAAATGTCGAGTGTGGATTAGGGTTTAGGATTTCACTTCAGTAGTTGAGGTATCCACCGGCGACAGTCCAATGGCAGAGCATTCCGCGCCGCCCGCTGACCAACTCGCCGCCGTCACCTTGAACGACGATGCTCCCACTTACAACTCTCTCAAAGCCGAATTCTCTGACCGCGTTCCGATCCGTTCAATTGTCTCCCGGCCCGATGGCGGCGCAGGCCTTGCCGGCCAGAAGGTTCGCGTTGGTGGCTGGGTCAAAACCGGAAGAAAAGCCGACAAGGATGCGTTTGCCTTTCTCGAGTTGAACGACGGCTCTTGCCCGCAGAATCTCCAAGTGATCATCGAAGCGTCTTTGGCGGATCTTGGCCAGATTGTCCCTACTGGAACCTGCGTTGTTGTCGATGGCGTCTTCAAGCTTCCTCCACCGGGTAAGCACCAGAAAGTCGAGCTTCGGGCTGATGCTGTCGTTCATGTCGGTCCGGTTGATCCCGCTCGGTATCCTTTACCGAAGACGAAACTCACTCTCGAGTTTCTAAGAGATTTCGTCCATCTCCGCCCTAGAACCAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACACACACATTTTTTAACAAGCATGGATTTCTTTATATTCACACACCAATTGTCACCACAAGCGATTGTGAGGGTGCTGGTGAAATGTTTCAAGTTACGACATTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGGTTAAGAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGCAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAAGCTGAAGGCATCTAAAGCTAGCAAGGAGGAAATTGGTGCTTCTGTTATGGAACTGAAGAAGGCAAAGGAGAATGTTTCTAAGCTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCGGAGAAAGATGGGAAAGTTGATTATGCAAAAGACTTCTTTGCTCGACAAGCGTTTTTGACTGTTTCGGGCCAGCTGCAAGTCGAAACATATGCCTGTGCTCTTAGTAGTGTATACACCTTTGGACCAACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGACGATATGAATTGTGCGGAGTCCTACGTGAGATTTTTGTGTCAATGGTTACTTGATAACTGCCTAGATGACATGCAATTTATGGCCGACAAGTACGACCGAACTTGTATTGATCGTCTCAAACTGGTCTCTTCAACCCCTTTTGTTCGAACTTCCTATACGGAAGCTGTTGAAATACTTGAAGAAGCTGTAAAGAAGGGTAAGGTGTTTGAGAACAAAGTGGAATGGGGGATTGATTTAGCTTCAGAGCATGAAAGATACTTGACTGAGGTAGTGTTTAAAAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCAAAAACTGTAGCTGCAATGGATGTTCTTGTACCAAAGGTTGGGGAGTTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGTGACAGGATCAAGGAGATAGATTTGCCACTTGAGCCATACGAATGGTATCTCGATCTGAGACGATATGGGACAGTGAAGCACAGCGGTTTTGGCCTCGGCTTCGAGCGAATGCTTCTTTTTGCCACTGGCATTGACAACATCAGAGATGTTATACCCTTCCCCAGATACCCAGGACGAGCAGACCTATGATCATATATATTTACACTAAAGAGAGATGTTGTTGGGTTTTAATTTATGCTCATAAATTTTAGATTGGTGGGTTTATTTTATACAGAAACTATTGCATTTCAAATGACTTAAATTGGTTTGATGTGTTAGATATTTTGGTACTAACAGATTTTAATTTGTTTACAGAGAAGATTGTAAAAAGAATGTAAAAGTGGATCAATGTCACAACAAGAGTCTGTTATGGGAGCGATTACTTCTACATTCATTTCACGTAAGACCCTAATTTTGCTTATGAATTTTTCGTTGAACTTTACTCGAATTTATTCCTTTTAGATTCATTCACTTTTAGCATTTTAAGGATTAAAATGAATATTTTT

Coding sequence (CDS)

ATGGCAGAGCATTCCGCGCCGCCCGCTGACCAACTCGCCGCCGTCACCTTGAACGACGATGCTCCCACTTACAACTCTCTCAAAGCCGAATTCTCTGACCGCGTTCCGATCCGTTCAATTGTCTCCCGGCCCGATGGCGGCGCAGGCCTTGCCGGCCAGAAGGTTCGCGTTGGTGGCTGGGTCAAAACCGGAAGAAAAGCCGACAAGGATGCGTTTGCCTTTCTCGAGTTGAACGACGGCTCTTGCCCGCAGAATCTCCAAGTGATCATCGAAGCGTCTTTGGCGGATCTTGGCCAGATTGTCCCTACTGGAACCTGCGTTGTTGTCGATGGCGTCTTCAAGCTTCCTCCACCGGGTAAGCACCAGAAAGTCGAGCTTCGGGCTGATGCTGTCGTTCATGTCGGTCCGGTTGATCCCGCTCGGTATCCTTTACCGAAGACGAAACTCACTCTCGAGTTTCTAAGAGATTTCGTCCATCTCCGCCCTAGAACCAACACGATATCTGCGGTTGCTAGAATTCGAAATGCACTTGCATATGCCACACACACATTTTTTAACAAGCATGGATTTCTTTATATTCACACACCAATTGTCACCACAAGCGATTGTGAGGGTGCTGGTGAAATGTTTCAAGTTACGACATTATTGAGTGAGGCTGAAAGGTTAGAAAAGGAGTTGGTTAAGAACCCACCTCCATCAGAAGCAGAAGTAGATGAAGCAAGGCAAATTGTCAAGGAGAAAGGAGAAGCTGTTGCCAAGCTGAAGGCATCTAAAGCTAGCAAGGAGGAAATTGGTGCTTCTGTTATGGAACTGAAGAAGGCAAAGGAGAATGTTTCTAAGCTGGAAGAGAGAGCAAAGCTTAAACCAGGCATTCCGGAGAAAGATGGGAAAGTTGATTATGCAAAAGACTTCTTTGCTCGACAAGCGTTTTTGACTGTTTCGGGCCAGCTGCAAGTCGAAACATATGCCTGTGCTCTTAGTAGTGTATACACCTTTGGACCAACCTTCCGTGCCGAAAATTCTCACACTTCAAGACATCTTGCAGAGTTCTGGATGGTTGAGCCTGAAATTGCATTTGCAGATTTGCAGGACGATATGAATTGTGCGGAGTCCTACGTGAGATTTTTGTGTCAATGGTTACTTGATAACTGCCTAGATGACATGCAATTTATGGCCGACAAGTACGACCGAACTTGTATTGATCGTCTCAAACTGGTCTCTTCAACCCCTTTTGTTCGAACTTCCTATACGGAAGCTGTTGAAATACTTGAAGAAGCTGTAAAGAAGGGTAAGGTGTTTGAGAACAAAGTGGAATGGGGGATTGATTTAGCTTCAGAGCATGAAAGATACTTGACTGAGGTAGTGTTTAAAAAGCCTGTCATTGTCTATAACTATCCAAAAGGAATCAAAGCATTTTATATGAGGCTCAATGACGACTCAAAAACTGTAGCTGCAATGGATGTTCTTGTACCAAAGGTTGGGGAGTTGATTGGTGGAAGCCAAAGGGAGGAGCGGTACGAGGTTATTCGTGACAGGATCAAGGAGATAGATTTGCCACTTGAGCCATACGAATGGTATCTCGATCTGAGACGATATGGGACAGTGAAGCACAGCGGTTTTGGCCTCGGCTTCGAGCGAATGCTTCTTTTTGCCACTGGCATTGACAACATCAGAGATGTTATACCCTTCCCCAGATACCCAGGACGAGCAGACCTATGA

Protein sequence

MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL

Homology

[Close]

BLAST of Carg20794 vs. NCBI nr
Match: XP_022961000.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 Asparagine--tRNA ligase, cytoplasmic 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. NCBI nr
Match: KAG6590322.1 (Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 571/572 (99.83%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAP ADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPTADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. NCBI nr
Match: XP_023520843.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1137.5 bits (2941), Expect = 0.0e+00
Identity = 570/572 (99.65%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEH APPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHPAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. NCBI nr
Match: XP_022988059.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima])

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. NCBI nr
Match: XP_038879816.1 (asparagine--tRNA ligase, cytoplasmic 1-like [Benincasa hispida])

HSP 1 Score: 1102.0 bits (2849), Expect = 0.0e+00
Identity = 547/572 (95.63%), Postives = 563/572 (98.43%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLN+DAPTY+SLKAEFSDRVPIR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNNDAPTYDSLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADL QIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLSQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGEAVA LKASKASKEEIGASV+ELKKAKENV+KLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEAVANLKASKASKEEIGASVLELKKAKENVAKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRF+CQWLLDNCLDDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRISYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEV+FKKPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 EVLEEAVKKGKEFENKVEWGIDLASEHERYLTEVIFKKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVI DRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIHDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. ExPASy Swiss-Prot
Match: Q9SW96 (Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 PE=1 SV=1)

HSP 1 Score: 859.0 bits (2218), Expect = 3.1e-248
Identity = 417/574 (72.65%), Postives = 494/574 (86.06%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MA+   PPA QLAAV+L +D  T    +A+FS+RV IR+I+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ SL D+  +V TGTCV VDGV K+PP GK
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA+LKA+KASKE I A+V ELK AKE  + ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+KDFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAE+YV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+E+LEEAV KGK F+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of Carg20794 vs. ExPASy Swiss-Prot
Match: Q9SSK1 (Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 PE=1 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 7.3e-242
Identity = 416/576 (72.22%), Postives = 485/576 (84.20%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           M +   PPA+QLAA  L +D  T    KA+FSDRV IRSI+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSILG---GGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++SL DL ++V TGTCV VDGV K+PP GK
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q +EL  + V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA+LK +KASKEEI ASV +L  AK +++ +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+ DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAE+YV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVEILEEAVKKGK-VFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK VF+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of Carg20794 vs. ExPASy Swiss-Prot
Match: Q6MEC9 (Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 GN=asnS PE=3 SV=1)

HSP 1 Score: 511.5 bits (1316), Expect = 1.2e-143
Identity = 272/541 (50.28%), Postives = 340/541 (62.85%), Query Frame = 0


Query: 34  RVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS 93
           R  I+S+         L G  + + GWV+T R  ++  F F+E+NDGS   N Q+I    
Sbjct: 2   RTKIKSLRGTTPEVRALIGHDITLKGWVRTVR--NQKTFTFIEINDGSTLSNFQIIATPD 61

Query: 94  LADLGQIV---PTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLT 153
           +A   Q++    TG  V   G   +  PGK Q +E++A A+  +G  DP  Y L K + T
Sbjct: 62  IAGYDQLINQLSTGVSVSAIGTI-VESPGKEQNLEMQATAITIIGKCDPEVYLLQKKRHT 121

Query: 154 LEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMF 213
            EFLR   HLRPRTNTI AV R+RNALA+ATH FF K GFLYIHTPI+T SDCEGAG+MF
Sbjct: 122 FEFLRSIAHLRPRTNTIGAVTRVRNALAFATHQFFQKRGFLYIHTPIITGSDCEGAGKMF 181

Query: 214 QVTTLLSEAERLEKELVKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVME 273
           QVTT           L +N P    E                                  
Sbjct: 182 QVTT-----------LDQNNPARTPE---------------------------------- 241

Query: 274 LKKAKENVSKLEERAKLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVY 333
                                    G+VDY +DFF +  +LTVSGQL  E YACALS VY
Sbjct: 242 -------------------------GRVDYTQDFFGKPTYLTVSGQLNGEIYACALSDVY 301

Query: 334 TFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAESYVRFLCQWLLDNCLDDMQF 393
           TFGPTFRAENS+TSRHLAEFWM+EPE+AFADL D+M+CAE Y++++ +++LDNC +DM+F
Sbjct: 302 TFGPTFRAENSNTSRHLAEFWMIEPEMAFADLNDNMDCAEDYLKYILKYVLDNCQEDMEF 361

Query: 394 MADKYDRTCIDRLKLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERY 453
                    I RL+ V +T F R SYT AV ILE+A KK   FE  V+WG+DL SEHER+
Sbjct: 362 FNKHVATDLISRLEHVINTSFERASYTYAVRILEKADKK---FEYPVKWGLDLQSEHERF 421

Query: 454 LTEVVFKKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIR 513
           L E  F KPVI+ +YPK IKAFYMR N+D+KTVAAMDVLVPKVGE+IGGSQREER  V+ 
Sbjct: 422 LAEEFFGKPVILTDYPKDIKAFYMRTNEDNKTVAAMDVLVPKVGEIIGGSQREERLSVLE 466

Query: 514 DRIKEIDLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRA 572
            ++KE +LP E Y WYL+LR++G+V HSGFG GFER++ F TG++NIRDVIPFPR+PG+A
Sbjct: 482 SKLKEFNLPAEEYWWYLELRKFGSVPHSGFGAGFERLVQFTTGMENIRDVIPFPRHPGKA 466

[Close]

BLAST of Carg20794 vs. ExPASy Swiss-Prot
Match: Q7MVE5 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) OX=242619 GN=asnS PE=3 SV=1)

HSP 1 Score: 497.3 bits (1279), Expect = 2.3e-139
Identity = 257/525 (48.95%), Postives = 327/525 (62.29%), Query Frame = 0


Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + +  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +   G  Q VE++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
             A+ RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGAIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 VKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAK 288
             NP                                             +++ + EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY KDFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE + ILEEAVK G  FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  E+ +P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of Carg20794 vs. ExPASy Swiss-Prot
Match: B2RK42 (Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 20709 / CIP 103683 / JCM 12257 / NCTC 11834 / 2561) OX=431947 GN=asnS PE=3 SV=1)

HSP 1 Score: 495.7 bits (1275), Expect = 6.8e-139
Identity = 256/525 (48.76%), Postives = 326/525 (62.10%), Query Frame = 0


Query: 49  GLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQI--VPTGTC 108
           GL GQ++ + GWV+T  K    A  F+ LNDGS   N+Q++ + +  D  Q+  + TG C
Sbjct: 17  GLVGQEINIKGWVRT--KRGNKAVNFIALNDGSTIHNMQIVADLASFDAAQMSQITTGAC 76

Query: 109 VVVDGVFKLPPPGKHQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNT 168
           + V G   +   G  Q VE++A A+   G  DPA YPL K   TLEFLR+  HLRPRTNT
Sbjct: 77  IGVIGTL-VESQGAGQSVEVQASAIEIYGTADPATYPLQKKGHTLEFLREIAHLRPRTNT 136

Query: 169 ISAVARIRNALAYATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL 228
              + RIR+ +A A HTFF+  G+ Y H P++T SDCEGAG+MFQVTTL           
Sbjct: 137 FGVIYRIRHHMAIAIHTFFHNKGYYYFHAPLITASDCEGAGQMFQVTTL----------- 196

Query: 229 VKNPPPSEAEVDEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAK 288
             NP                                             +++ + EE   
Sbjct: 197 --NP---------------------------------------------DSLPRTEE--- 256

Query: 289 LKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRH 348
                    G+VDY KDFF R   LTVSGQL+ E  A AL  +YTFGPTFRAENS+T RH
Sbjct: 257 ---------GQVDYRKDFFGRHTSLTVSGQLEGEMAAMALGGIYTFGPTFRAENSNTPRH 316

Query: 349 LAEFWMVEPEIAFADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLV 408
           LAEFWM+EPE+AF +++D+M+ AE ++++  QW LDNC+DD+ F+++ +D+  IDRLK V
Sbjct: 317 LAEFWMIEPEVAFLEIEDNMDLAEEFIKYCVQWALDNCMDDISFLSEHFDKELIDRLKFV 376

Query: 409 SSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYP 468
              PFVR +YTE + ILEEAVK G  FE  + WG DLASEHERYL EV FK PVI+ +YP
Sbjct: 377 LEKPFVRLAYTEGIRILEEAVKNGVKFEFPIYWGADLASEHERYLVEVHFKTPVIMTDYP 436

Query: 469 KGIKAFYMRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWY 528
           K IK+FYM+LNDD KTV  MDVL PK+GE+IGGS+RE  Y+ +  R  E+ +P +   WY
Sbjct: 437 KEIKSFYMKLNDDGKTVRGMDVLFPKIGEIIGGSEREADYDKLVARANEMGVPEKDIWWY 468

Query: 529 LDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRAD 572
           LD RRYGT  HSGFGLGFER+LLF TG+ NIRDVIPFPR P  A+
Sbjct: 497 LDSRRYGTAPHSGFGLGFERLLLFVTGMSNIRDVIPFPRTPNNAE 468

[Close]

BLAST of Carg20794 vs. ExPASy TrEMBL
Match: A0A6J1HAP9 (Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1)

HSP 1 Score: 1140.6 bits (2949), Expect = 0.0e+00
Identity = 572/572 (100.00%), Postives = 572/572 (100.00%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. ExPASy TrEMBL
Match: A0A6J1JKK1 (Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1)

HSP 1 Score: 1135.9 bits (2937), Expect = 0.0e+00
Identity = 569/572 (99.48%), Postives = 571/572 (99.83%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVG VDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGSVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY
Sbjct: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
           AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEVDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. ExPASy TrEMBL
Match: A0A6J1DMU4 (Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111022537 PE=3 SV=1)

HSP 1 Score: 1094.7 bits (2830), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 563/572 (98.43%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAE+SAPPADQLAA TLNDDAPTY +LKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAENSAPPADQLAAATLNDDAPTYKALKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEA++ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAAVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
            QKVELRA+AVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 QQKVELRAEAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAE+LEKEL+KNPPPSEA+V+ A
Sbjct: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAEKLEKELIKNPPPSEADVEAA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R+IVKEKGE VAKLKA+KASKEEIGASV+ELKKAKENV++LEER+KLKPGIPEKDGKVDY
Sbjct: 241 RKIVKEKGEDVAKLKAAKASKEEIGASVLELKKAKENVARLEERSKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            +DFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 GRDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRFLCQWLLDNCLDDM+FMADKYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFLCQWLLDNCLDDMKFMADKYDRTCIDRLKMVSSTPFVRISYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           EILEEAVKKGKVFENKVEWGIDLASEHERYLTEV FKKPVIVYNYPKGIKAFYMRLNDDS
Sbjct: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVEFKKPVIVYNYPKGIKAFYMRLNDDS 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGEL+GGSQREERYEVIRDRIKE+DLPLEPYEWYLDLRRYGTVKHSGF
Sbjct: 481 KTVAAMDVLVPKVGELVGGSQREERYEVIRDRIKEMDLPLEPYEWYLDLRRYGTVKHSGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. ExPASy TrEMBL
Match: A0A5D3CE86 (Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G003180 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 558/572 (97.55%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAVTLN+DA  Y SLKAEFSDRVPIR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRF+CQWLLDNCLDDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. ExPASy TrEMBL
Match: A0A1S3BPW6 (Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103492241 PE=3 SV=1)

HSP 1 Score: 1092.4 bits (2824), Expect = 0.0e+00
Identity = 542/572 (94.76%), Postives = 558/572 (97.55%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MAEHSAPP DQLAAVTLN+DA  Y SLKAEFSDRVPIR+IVSRPDGGAGLAGQKVRVGGW
Sbjct: 1   MAEHSAPPTDQLAAVTLNNDASAYESLKAEFSDRVPIRTIVSRPDGGAGLAGQKVRVGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGRKADKDAFAFLELNDGSCPQNLQVIIEAS+ADLGQIVPTGTCVVVDGVFKLPPPGK
Sbjct: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASVADLGQIVPTGTCVVVDGVFKLPPPGK 120

Query: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 180
           HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRD VHLRPRTNTISAVARIRNALAYA
Sbjct: 121 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDVVHLRPRTNTISAVARIRNALAYA 180

Query: 181 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEA 240
           THTFFN HGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKEL+KNPPPSEAEVDEA
Sbjct: 181 THTFFNNHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELIKNPPPSEAEVDEA 240

Query: 241 RQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDY 300
           R IVKEKGEAVA LKA+KASKEEIGASV+ELKKAKENV++LEERAKLKPGIPEKDGKVDY
Sbjct: 241 RNIVKEKGEAVASLKAAKASKEEIGASVLELKKAKENVARLEERAKLKPGIPEKDGKVDY 300

Query: 301 AKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360
            KDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA
Sbjct: 301 TKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFA 360

Query: 361 DLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAV 420
           DLQDDMNCAE+YVRF+CQWLLDNCLDDM+FMA+KYDRTCIDRLK+VSSTPFVR SYTEAV
Sbjct: 361 DLQDDMNCAEAYVRFMCQWLLDNCLDDMKFMAEKYDRTCIDRLKMVSSTPFVRVSYTEAV 420

Query: 421 EILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLNDDS 480
           E+LEEAVKKGK FENKVEWGIDLASEHERYLTEVVF+KPVIVYNYPKGIKAFYMRLNDD 
Sbjct: 421 ELLEEAVKKGKEFENKVEWGIDLASEHERYLTEVVFRKPVIVYNYPKGIKAFYMRLNDDL 480

Query: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGF 540
           KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKE+ LPLEPYEWYLDLRRYGTVKH GF
Sbjct: 481 KTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEMGLPLEPYEWYLDLRRYGTVKHCGF 540

Query: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GLGFERMLLFATGIDNIRDVIPFPRYPGRADL
Sbjct: 541 GLGFERMLLFATGIDNIRDVIPFPRYPGRADL 572

[Close]

BLAST of Carg20794 vs. TAIR 10
Match: AT5G56680.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 859.0 bits (2218), Expect = 2.2e-249
Identity = 417/574 (72.65%), Postives = 494/574 (86.06%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           MA+   PPA QLAAV+L +D  T    +A+FS+RV IR+I+ RPDGGAGLAGQ VR+GGW
Sbjct: 1   MADEIVPPATQLAAVSLENDGSTVQ--RAQFSNRVLIRTILDRPDGGAGLAGQTVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VK+GR   K  F+FL +NDGSCP NLQV+++ SL D+  +V TGTCV VDGV K+PP GK
Sbjct: 61  VKSGRDQGKRTFSFLAVNDGSCPANLQVMVDPSLYDVSNLVATGTCVTVDGVLKVPPKGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q++EL    V+ VG VD ++YPLPKTKLTLE LRD +HLR RTN+ISAVARIRNALA
Sbjct: 121 GTQQQIELNVVKVIDVGTVDASKYPLPKTKLTLETLRDVLHLRSRTNSISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQ TTL++  ERLE++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQATTLINYTERLEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IV E+G  VA+LKA+KASKE I A+V ELK AKE  + ++ER++L+PG+P+KDG +
Sbjct: 241 AARLIVIERGNVVAELKAAKASKEAITAAVAELKIAKETFAHIDERSRLRPGLPKKDGNI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+KDFF RQAFLTVSGQLQVETYACALS+VYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSKDFFGRQAFLTVSGQLQVETYACALSNVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FADL+DDMNCAE+YV+++C WLL+ C  DM+ MA  +D  CIDRLKLV+STPF R +YT+
Sbjct: 361 FADLEDDMNCAEAYVKYMCNWLLEKCYADMELMAKNFDSGCIDRLKLVASTPFGRITYTK 420

Query: 421 AVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLND 480
           A+E+LEEAV KGK F+N VEWGIDLASEHERYLTEV+F+KP+IVYNYPKGIKAFYMRLND
Sbjct: 421 AIELLEEAVAKGKEFDNNVEWGIDLASEHERYLTEVLFQKPLIVYNYPKGIKAFYMRLND 480

Query: 481 DSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHS 540
           D KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVKH 
Sbjct: 481 DEKTVAAMDVLVPKVGELIGGSQREERYDVIKKRIEEMGLPIEPYEWYLDLRRYGTVKHC 540

Query: 541 GFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           GFGLGFERM+LFATG+DNIRDVIPFPRYPG+ADL
Sbjct: 541 GFGLGFERMILFATGLDNIRDVIPFPRYPGKADL 572

[Close]

BLAST of Carg20794 vs. TAIR 10
Match: AT1G70980.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 837.8 bits (2163), Expect = 5.2e-243
Identity = 416/576 (72.22%), Postives = 485/576 (84.20%), Query Frame = 0


Query: 1   MAEHSAPPADQLAAVTLNDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGW 60
           M +   PPA+QLAA  L +D  T    KA+FSDRV IRSI+    GGA LAGQKVR+GGW
Sbjct: 1   MGDEIVPPANQLAADNLENDGSTVQ--KAQFSDRVLIRSILG---GGAKLAGQKVRIGGW 60

Query: 61  VKTGRKADKDAFAFLELNDGSCPQNLQVIIEASLADLGQIVPTGTCVVVDGVFKLPPPGK 120
           VKTGR+  K  FAFLE+NDGSCP NLQV++++SL DL ++V TGTCV VDGV K+PP GK
Sbjct: 61  VKTGRQQGKGTFAFLEVNDGSCPANLQVMVDSSLYDLSRLVATGTCVTVDGVLKIPPEGK 120

Query: 121 --HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALA 180
              Q +EL  + V+ VG VDP  YPLPKTKLT EFLRD +HLR RTN ISAVARIRNALA
Sbjct: 121 GLKQSIELSVETVIAVGTVDPTTYPLPKTKLTPEFLRDVLHLRSRTNLISAVARIRNALA 180

Query: 181 YATHTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVD 240
           +ATH+FF +H FLYIHTPI+TTSDCEGAGEMFQVTTL++  ER+E++L+ NPPP+EA+V+
Sbjct: 181 FATHSFFQEHSFLYIHTPIITTSDCEGAGEMFQVTTLINHTERVEQDLIDNPPPTEADVE 240

Query: 241 EARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKV 300
            AR IVKE+GEAVA+LK +KASKEEI ASV +L  AK +++ +EER +LKPG+P+ DGK+
Sbjct: 241 AARLIVKERGEAVAQLKVAKASKEEITASVAQLSVAKASLAHVEERLRLKPGLPKNDGKI 300

Query: 301 DYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360
           DY+ DFF RQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA
Sbjct: 301 DYSNDFFGRQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIA 360

Query: 361 FADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTE 420
           FAD+ DDMNCAE+YV+++C+WL+D C DDM+ M    D  C  RL +V+   F R +YTE
Sbjct: 361 FADIHDDMNCAEAYVKYMCKWLMDKCGDDMELMDKNVDEGCTKRLNMVAKASFKRVTYTE 420

Query: 421 AVEILEEAVKKGK-VFENKVEWGIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRL 480
           A+E LE+AV +GK VF+NKVEWGIDLASEHERYLTEV F +KP+IVYNYPKGIKAFYMRL
Sbjct: 421 AIERLEKAVAQGKVVFDNKVEWGIDLASEHERYLTEVEFDQKPIIVYNYPKGIKAFYMRL 480

Query: 481 NDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVK 540
           NDD KTVAAMDVLVPKVGELIGGSQREERY+VI+ RI+E+ LP+EPYEWYLDLRRYGTVK
Sbjct: 481 NDDEKTVAAMDVLVPKVGELIGGSQREERYDVIKQRIEEMGLPMEPYEWYLDLRRYGTVK 540

Query: 541 HSGFGLGFERMLLFATGIDNIRDVIPFPRYPGRADL 573
           H GFGLGFERM+ FATGIDNIRDVIPFPRYPG+ADL
Sbjct: 541 HCGFGLGFERMIQFATGIDNIRDVIPFPRYPGKADL 571

[Close]

BLAST of Carg20794 vs. TAIR 10
Match: AT4G17300.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 490.3 bits (1261), Expect = 2.0e-138
Identity = 265/553 (47.92%), Postives = 346/553 (62.57%), Query Frame = 0


Query: 24  YNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDAFAFLELNDGSCP 83
           + S   EF  ++ I  +    D G    GQ + + GWV+T R   + +  F+E+NDGSC 
Sbjct: 81  FGSKVGEFRKKLRIAEVKGGADEGLSRVGQSLNIMGWVRTLR--SQSSVTFIEINDGSCL 140

Query: 84  QNLQVIIEASLADLGQI----VPTGTCVVVDGVFKLPPPGKHQKVELRADAVVHVGPVDP 143
            NLQ ++ +      Q+    + TG  V V G   +   G  QKVEL+ + ++ VG  D 
Sbjct: 141 SNLQCVMTSDAEGYDQVESGSILTGASVSVQGTI-VASQGTKQKVELKVEKIIVVGECD- 200

Query: 144 ARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYATHTFFNKHGFLYIHTPIVT 203
           + YP+ K +++ EFLR   HLRPRTNT  AVAR+RN LAYATH FF + GF+++ +PI+T
Sbjct: 201 SSYPIQKKRVSREFLRTKAHLRPRTNTFGAVARVRNTLAYATHKFFQESGFVWVASPIIT 260

Query: 204 TSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAEVDEARQIVKEKGEAVAKLKASKA 263
            SDCEGAGE F VTTL+                                          +
Sbjct: 261 ASDCEGAGEQFCVTTLI-----------------------------------------PS 320

Query: 264 SKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQV 323
           S E    S+  + K                    K G +D+++DFF + AFLTVSGQL  
Sbjct: 321 SHENTDTSIDAIPKT-------------------KGGLIDWSQDFFGKPAFLTVSGQLNG 380

Query: 324 ETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEIAFADLQDDMNCAESYVRFLCQW 383
           ETYA ALS VYTFGPTFRAENS+TSRHLAEFWM+EPE+AFADL DDM CA +Y++++ ++
Sbjct: 381 ETYATALSDVYTFGPTFRAENSNTSRHLAEFWMIEPELAFADLDDDMACATAYLQYVVKY 440

Query: 384 LLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEW 443
           +LDNC +DM+F     ++  I RL  V+   F++  YT+A+EIL +A KK   F+  V+W
Sbjct: 441 VLDNCKEDMEFFDTWIEKGIIRRLSDVAEKEFLQLGYTDAIEILLKANKK---FDFPVKW 500

Query: 444 GIDLASEHERYLTEVVF-KKPVIVYNYPKGIKAFYMRLNDDSKTVAAMDVLVPKVGELIG 503
           G+DL SEHERY+TE  F  +PVI+ +YPK IKAFYMR NDD KTVAAMD+LVP++GELIG
Sbjct: 501 GLDLQSEHERYITEEAFGGRPVIIRDYPKEIKAFYMRENDDGKTVAAMDMLVPRIGELIG 560

Query: 504 GSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIR 563
           GSQREER EV+  R+ E+ L  E Y WYLDLRRYG+V H+GFGLGFER++ F TGIDNIR
Sbjct: 561 GSQREERLEVLEARLDELKLNKESYWWYLDLRRYGSVPHAGFGLGFERLVQFVTGIDNIR 566

Query: 564 DVIPFPRYPGRAD 572
           DVIPFPR P  A+
Sbjct: 621 DVIPFPRTPASAE 566

[Close]

BLAST of Carg20794 vs. TAIR 10
Match: AT3G07420.1 (asparaginyl-tRNA synthetase 2 )

HSP 1 Score: 477.6 bits (1228), Expect = 1.4e-134
Identity = 264/634 (41.64%), Postives = 384/634 (60.57%), Query Frame = 0


Query: 18  NDDAPTYNSLKAEFSDRVPIRSIVSRPDGGAGLAGQKVRVGGWVKTGRKADKDA------ 77
           ND +P   +L +++S RV +++++ R D GAGLAG++V +GGWVK+ R   K++      
Sbjct: 14  NDLSPKPITL-SKYSKRVELKTLLDRSDRGAGLAGKRVVIGGWVKSARAVKKNSPPPPLP 73

Query: 78  ------------------------------------------------------------ 137
                                                                       
Sbjct: 74  VVAAPSPSSGGDQAHTTANIRCTEIIQSKMNIFKRFFDVLSGGGKTYPIFDKTELAGQKA 133

Query: 138 -------FAFLELNDGSCPQNLQVIIEASLADL--GQIVPTGTCVVVDGVFKLP--PPGK 197
                  F FL ++DGS   +LQV+++++L+ +   Q++  GTC+V +GV +LP     K
Sbjct: 134 VPPPEYVFYFL-ISDGSSISSLQVVVDSALSTVPATQLMALGTCIVAEGVLRLPLAASAK 193

Query: 198 HQKVELRADAVVHVGPVDPARYPLPKTKLTLEFLRDFVHLRPRTNTISAVARIRNALAYA 257
           H  +EL A+ ++HVG VDP +YPL K +L L  LRDF H RPRT T+ +V R+ +AL  A
Sbjct: 194 H-VIELEAEKLLHVGTVDPEKYPLSKKQLPLHMLRDFSHFRPRTTTVGSVTRVHSALTLA 253

Query: 258 THTFFNKHGFLYIHTPIVTTSDCEGAGEMFQVTTLLSEAERLEKELVKNPPPSEAE---V 317
           +HTF   HGF Y+  P++TT+   G GEMF+VTTLL + +  E+   K PP  E +   +
Sbjct: 254 SHTFLQYHGFQYVQVPVITTT--TGFGEMFRVTTLLGKTDDKEE---KKPPVQEKDGFSI 313

Query: 318 DEARQIVKEKGEAVAKLKASKASKEEIGASVMELKKAKENVSKLEERAKLKPGIPEKDGK 377
           D  + ++KEK   +  LK S +++E + A+V +LKK  +  S++E + K K G   K  K
Sbjct: 314 DTVKAVIKEKTRLIDHLKRSDSNRETVVAAVHDLKKTNDLASQIEMKQKSKTGTLVKPEK 373

Query: 378 VDYAKDFFARQAFLTVSGQLQVETYACALSSVYTFGPTFRAENSHTSRHLAEFWMVEPEI 437
           +D++KDFF R  +LT SG+  +E+YA AL  VYTFGP F A+    +RHLAE W VE E+
Sbjct: 374 LDFSKDFFGRDTYLTASGRFHLESYASALGKVYTFGPRFIADKIDNARHLAEKWNVETEM 433

Query: 438 AFADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRLKLVSSTPFVRTSYT 497
           AFA+L D M+CA+ Y +FLC+++L+N  +DM+F++ + D+T   RL+  +S+  +R SYT
Sbjct: 434 AFAELDDAMDCADEYFKFLCKYVLENRDEDMKFISKRVDKTITTRLEATASSSLLRFSYT 493

Query: 498 EAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKPVIVYNYPKGIKAFYMRLN 557
           E + +L++A      FE K EWG+ L +EH  YLT+ ++K PVIV+ YPK IK FY+RLN
Sbjct: 494 EVISLLQKATT--TKFETKPEWGVALTTEHLSYLTDEIYKGPVIVHTYPKAIKQFYVRLN 553

Query: 558 DDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDLPLEPYEWYLDLRRYGTVKH 572
           DD KTVAA D++VPKVG +I GSQ EER+E++  RI E     E +EWYLDLRR+GTVKH
Sbjct: 554 DDKKTVAAFDLVVPKVGVVITGSQNEERFEILDARIGESGFTREKFEWYLDLRRHGTVKH 613

[Close]

BLAST of Carg20794 vs. TAIR 10
Match: AT4G31180.1 (Class II aminoacyl-tRNA and biotin synthetases superfamily protein )

HSP 1 Score: 117.1 bits (292), Expect = 4.7e-26
Identity = 90/291 (30.93%), Postives = 144/291 (49.48%), Query Frame = 0


Query: 286 KLKPGIPEKDGKVDYAKDFFARQAFLTVSGQLQVETYACA-LSSVYTFGPTFRAENSHTS 345
           KL  G  E  G   +  ++  + A L  S QL  +   C  L  V+  GP FRAE+S T 
Sbjct: 279 KLMAGSSE-GGSAVFRLEYKGQPACLAQSPQLHKQMAICGDLRRVFEVGPVFRAEDSFTH 338

Query: 346 RHLAEFWMVEPEIAF-ADLQDDMNCAESYVRFLCQWLLDNCLDDMQFMADKYDRTCIDRL 405
           RHL EF  ++ E+       + M+  +    F+   L + C  ++Q +  +Y     + L
Sbjct: 339 RHLCEFVGLDVEMEIRKHYSEIMDLVDELFVFIFTSLNERCKKELQAVGKQYP---FEPL 398

Query: 406 KLVSSTPFVRTSYTEAVEILEEAVKKGKVFENKVEWGIDLASEHERYLTEVVFKKP---- 465
           K +  T  +R ++ E V++L+EA         +V+   DL +E ER L ++V +K     
Sbjct: 399 KFLPKT--LRLTFEEGVQMLKEA-------GVEVDPLGDLNTESERKLGQLVLEKYNTEF 458

Query: 466 VIVYNYPKGIKAFY-MRLNDDSKTVAAMDVLVPKVGELIGGSQREERYEVIRDRIKEIDL 525
            I++ YPK ++ FY M   D+     + DV + +  E+I G+QR    EV+  R  E  +
Sbjct: 459 YILHRYPKAVRPFYTMTCADNPLYSNSFDVFI-RGEEIISGAQRVHIPEVLEQRAGECGI 518

Query: 526 PLEPYEWYLDLRRYGTVKHSGFGLGFERMLLFATGIDNIRDVIPFPRYPGR 570
            ++    Y+D  RYG   H GFG+G ER+++    ++NIR    FPR P R
Sbjct: 519 DVKTISTYIDSFRYGAPLHGGFGVGLERVVMLFCALNNIRKTSLFPRDPQR 555

The following BLAST results are available for this feature:

BLAST of Carg20794 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022961000.1	0.0e+00	100.00	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita moschata] >KAG7023917.1 A...	[more]
KAG6590322.1	0.0e+00	99.83	Asparagine--tRNA ligase, cytoplasmic 1, partial [Cucurbita argyrosperma subsp. s...	[more]
XP_023520843.1	0.0e+00	99.65	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022988059.1	0.0e+00	99.48	asparagine--tRNA ligase, cytoplasmic 1-like [Cucurbita maxima]	[more]
XP_038879816.1	0.0e+00	95.63	asparagine--tRNA ligase, cytoplasmic 1-like [Benincasa hispida]	[more]

BLAST of Carg20794 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SW96	3.1e-248	72.65	Asparagine--tRNA ligase, cytoplasmic 1 OS=Arabidopsis thaliana OX=3702 GN=SYNC1 ...	[more]
Q9SSK1	7.3e-242	72.22	Asparagine--tRNA ligase, cytoplasmic 3 OS=Arabidopsis thaliana OX=3702 GN=SYNC3 ...	[more]
Q6MEC9	1.2e-143	50.28	Asparagine--tRNA ligase OS=Protochlamydia amoebophila (strain UWE25) OX=264201 G...	[more]
Q7MVE5	2.3e-139	48.95	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC BAA-308 / W83) ...	[more]
B2RK42	6.8e-139	48.76	Asparagine--tRNA ligase OS=Porphyromonas gingivalis (strain ATCC 33277 / DSM 207...	[more]

BLAST of Carg20794 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HAP9	0.0e+00	100.00	Asparagine--tRNA ligase OS=Cucurbita moschata OX=3662 GN=LOC111461633 PE=3 SV=1	[more]
A0A6J1JKK1	0.0e+00	99.48	Asparagine--tRNA ligase OS=Cucurbita maxima OX=3661 GN=LOC111485413 PE=3 SV=1	[more]
A0A6J1DMU4	0.0e+00	94.76	Asparagine--tRNA ligase OS=Momordica charantia OX=3673 GN=LOC111022537 PE=3 SV=1	[more]
A0A5D3CE86	0.0e+00	94.76	Asparagine--tRNA ligase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold...	[more]
A0A1S3BPW6	0.0e+00	94.76	Asparagine--tRNA ligase OS=Cucumis melo OX=3656 GN=LOC103492241 PE=3 SV=1	[more]

BLAST of Carg20794 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G56680.1	2.2e-249	72.65	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT1G70980.1	5.2e-243	72.22	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT4G17300.1	2.0e-138	47.92	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]
AT3G07420.1	1.4e-134	41.64	asparaginyl-tRNA synthetase 2	[more]
AT4G31180.1	4.7e-26	30.93	Class II aminoacyl-tRNA and biotin synthetases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	COILS	Coil	Coil	coord: 264..284
None	No IPR available	GENE3D	3.30.930.10	Bira Bifunctional Protein; Domain 2	coord: 143..571 e-value: 1.3E-106 score: 358.7
None	No IPR available	GENE3D	2.40.50.140		coord: 33..138 e-value: 4.7E-10 score: 41.2
None	No IPR available	GENE3D	1.10.287.10		coord: 230..282 e-value: 5.1E-5 score: 25.1
None	No IPR available	PANTHER	PTHR22594	ASPARTYL/LYSYL-TRNA SYNTHETASE	coord: 1..572
None	No IPR available	PANTHER	PTHR22594:SF52	BNAC03G13340D PROTEIN	coord: 1..572
None	No IPR available	CDD	cd00776	AsxRS_core	coord: 148..568 e-value: 1.37712E-143 score: 415.812
None	No IPR available	CDD	cd04318	EcAsnRS_like_N	coord: 54..135 e-value: 9.20831E-26 score: 98.7916
None	No IPR available	SUPERFAMILY	55681	Class II aaRS and biotin synthetases	coord: 151..567
IPR002312	Aspartyl/Asparaginyl-tRNA synthetase, class IIb	PRINTS	PR01042	TRNASYNTHASP	coord: 327..340 score: 43.83 coord: 487..503 score: 44.79 coord: 311..323 score: 40.73 coord: 527..541 score: 47.27
IPR000738	WHEP-TRS domain	SMART	SM00991	WHEP_TRS_a_2_a_3_a	coord: 240..297 e-value: 0.0065 score: 25.3
IPR000738	WHEP-TRS domain	PROSITE	PS51185	WHEP_TRS_2	coord: 236..292 score: 10.268002
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 152..214 e-value: 3.4E-12 score: 46.1
IPR004364	Aminoacyl-tRNA synthetase, class II (D/K/N)	PFAM	PF00152	tRNA-synt_2	coord: 301..565 e-value: 9.9E-63 score: 212.1
IPR004522	Asparagine-tRNA ligase	TIGRFAM	TIGR00457	TIGR00457	coord: 49..571 e-value: 8.2E-167 score: 553.5
IPR004522	Asparagine-tRNA ligase	HAMAP	MF_00534	Asn_tRNA_synth	coord: 37..572 score: 42.136696
IPR006195	Aminoacyl-tRNA synthetase, class II	PROSITE	PS50862	AA_TRNA_LIGASE_II	coord: 329..562 score: 13.63026
IPR012340	Nucleic acid-binding, OB-fold	SUPERFAMILY	50249	Nucleic acid-binding proteins	coord: 48..135

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20794-RA	Carg20794-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006421	asparaginyl-tRNA aminoacylation
biological_process	GO:0006418	tRNA aminoacylation for protein translation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0004816	asparagine-tRNA ligase activity
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0003677	DNA binding
molecular_function	GO:0004812	aminoacyl-tRNA ligase activity
molecular_function	GO:0000166	nucleotide binding