Carg20749 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20749
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	WAT1-related protein
Location	Carg_Chr15: 10093872 .. 10096526 (-)
RNA-Seq Expression	Carg20749
Synteny	Carg20749

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGTCAGGGGTTGGATCATCAGGGGTTGGATCATCAGGCACCACAATATGCACCAATGGCGCCTTCCATGCCACTGCCAAACCCGCCCTTCTCATGCTTCTGGTTCAGTCGGTGTTCGCAGGCGTCAACGTTCTCTACAAATTGGCTGTCAACGATGGAATGAATCTGATGATCATGATCGCTTTTCGTTTCGTGTTCGCTTCCCTTTTCATGCTTCCCCTTGCCTTCTTTCTCGAAAGGTTTTTTCTTTTCGTCTACATTGAGTTTTCCCTTTAAAATAAGGAGACATTTTCGTATCATTATGTGTCCGTTCTAATTTAAATTTCTAACAGGAATAAACGCCCGAAGATGACATGCTCCATACTCTTCTATGGATTTCTTTGTGGATTATTTGGGTACGTAAGCTAAATTAATAAAAATACTCGTTACAATTAATTTTACATGAAAATATTTTTATTAGAAGGTGTACGGGAACAAAAATGAATAAGAAGTGCAATTTATTTGGACAGAGGGACGTTGAGTCAAAACTTGTACGTACAGAGTTTGGCAATGACGTCAGCAACATTTGTTTCGGCCATGCAGAATCTCTCTCCGGCTATTACCTTCCTTCTCGCCCTCTCCTTCGGGTAACAATATCCACATCTCTTATGCCTAACATAAACAAATAAACTTAAATTTTTGTTGTTTAGTTTGAGTTAGGAGCGTAGACGAGTTAGAGTTGAATTCAGTTGATTATTCGAATCAATTAAACAGGGTCTATTATTCAATCCAATCCAATCAATAGCCTCATTATTTATTTTAGAAACTTTTAATTATCTACGTTAATAACTAAACTCTTATTAAACTTCATATTTCAATATTCACAAAATTGAAGAAATAAACCCTAACATATAACAAATGTTAAATTTTACTTTGGATCGAGTTGAACCGATCCAAAAACCCTAATTTGCCAACCAAACCAAAACCCAACTCCAAAACGAGAAAAAAAAAAAAAAAAAAAAGTCAACTCGACTTTTAGAATTTAGATTAGACAGCTCGAGTCGTTTAGATTATTGGGTCATTTGAATTGTATATTTAATCGTTCCTAAACACTAAAACAACTACTATCAAAATTTCTTATTGTATTTTATATCAAAATTTCATGAATGTACACTTTTTTTTTCTTATTGTATTTTTTTTTTTTATAAACTTACAAAATTAGGTATGATAATTTAGAATTAAAATTTATAAATAAAAAAGAAAAAAAAAGATTTATGAGCATGTATATATGATGATGATGTATCACAATCACTTTATTAAATAATAATAATAATAATAAAAAGTTAAAAAGGATGACCATATGTTTACTTGGAAGTCCTTCTACTTTGGGGTAACCCATCAAAACCCTTTTTTGGAAAATCAGAGAAAAACTTCAATTTGATTACGAGTGATGTAAAATCTACGAACCTTTTAACGTAGCAACTCAATTCAACTTTGAATATTGGAAAAAGAGAAGAAAAAAAAAGTAGCTTTAGATACATGAGGTGTTTGATTTACAGTCGAGATGGCTGTCTAAAAGTAATGGACACTCGAGAATAAGATGTCAAAATATAAAATAATATTATGTTTGATTATACTCGATAATGCTGATAAATTTCTTAAAATTCTAAGAATTTTTATATTAATTAATCATTTCGTTAATGAATTTGATCTAAACATAAGTTTGTTGCAATACTATTAAAATTATATTTTTTGTATTGCAATTTAGTAATTTTATTTTGAGATAAGATGAAAATTAATTAATTAAGGTTATGAGGTTAAGGTGTACGGACATGTGTAGGATGGAAAAGTTGGAGATGAGGAGCAAGGAAGGGGTGGCCAAGGTGGCAGGAACGTTAGTGGGGATTGGTGGGGCCATGTTTCTTACTTTCTACAAAGGCATCCAGATCAGCATTTGGTCAACCCACGTGGACCTCCTCCGTGGAGGACACTTGGCACATCTTCCTCAAAATCCTCACCACTCCCACAATTTTGTGCTTGGAAGTCTGCTGGCTCTCGCCAGCTGCCTCTCTTACTCTTTCTGGCTCATCCTTCAGGTTACCATTGTTGATGCTTCTTTATTCTCTGAACTAAAAATTGAATTTGAGTACACCTAAGTGTGGATAAGACATAAAATATTAATCTAAATGGAGAAAGATATACTTAGATTTCTCAGACGTAATCGACTACTATTCAATAGATCTTATATGTAAGATTTGATTTTGGTGGCAGACAAAAATGACGAAAATATATCCATGCCAATACTCGAGCACCGCGTTGATGTGTGTGATGGGAGCAATTCAGGGGTTGGCGATATCAATTTGTGTCGAGAGGGATTGGAAGCAATGGAAATTGGGTTGGAATATCAGGCTTCTCACCGTTGCATTTGCCGTAATTTATTAATTTTTAAGTCTTTTAAACATTTAGATATGATCCTTTGGTTCACTTAATAATACGACATACATATAATGGATTTGGATGCAGGGGATCGTGGCGTCGGGAGCCATGGTGACGCTGATGGCGTGGTGCGTACGTATGAGAGGGCCACTGTATGTGTCTATTTTCAGCCCTCTTATGCTTCTCCTGGTAGCTATTGCTGGATCCCTATGCCTCCAAGAAAGCTTGCACCTTGGC

mRNA sequence

ATGGAGTCAGGGGTTGGATCATCAGGGGTTGGATCATCAGGCACCACAATATGCACCAATGGCGCCTTCCATGCCACTGCCAAACCCGCCCTTCTCATGCTTCTGGTTCAGTCGGTGTTCGCAGGCGTCAACGTTCTCTACAAATTGGCTGTCAACGATGGAATGAATCTGATGATCATGATCGCTTTTCGTTTCGTGTTCGCTTCCCTTTTCATGCTTCCCCTTGCCTTCTTTCTCGAAAGGATGGAAAAGTTGGAGATGAGGAGCAAGGAAGGGGTGGCCAAGGTGGCAGGAACGTTAGTGGGGATTGGTGGGGCCATGTTTCTTACTTTCTACAAAGGCATCCAGATCAGCATTTGGTCAACCCACGTGGACCTCCTCCGTGGAGGACACTTGGCACATCTTCCTCAAAATCCTCACCACTCCCACAATTTTGTGCTTGGAAGTCTGCTGGCTCTCGCCAGCTGCCTCTCTTACTCTTTCTGGCTCATCCTTCAGACAAAAATGACGAAAATATATCCATGCCAATACTCGAGCACCGCGTTGATGTGTGTGATGGGAGCAATTCAGGGGTTGGCGATATCAATTTGTGTCGAGAGGGATTGGAAGCAATGGAAATTGGGTTGGAATATCAGGCTTCTCACCGTTGCATTTGCCGGGATCGTGGCGTCGGGAGCCATGGTGACGCTGATGGCGTGGTGCGTACGTATGAGAGGGCCACTGTATGTGTCTATTTTCAGCCCTCTTATGCTTCTCCTGGTAGCTATTGCTGGATCCCTATGCCTCCAAGAAAGCTTGCACCTTGGC

Coding sequence (CDS)

ATGGAGTCAGGGGTTGGATCATCAGGGGTTGGATCATCAGGCACCACAATATGCACCAATGGCGCCTTCCATGCCACTGCCAAACCCGCCCTTCTCATGCTTCTGGTTCAGTCGGTGTTCGCAGGCGTCAACGTTCTCTACAAATTGGCTGTCAACGATGGAATGAATCTGATGATCATGATCGCTTTTCGTTTCGTGTTCGCTTCCCTTTTCATGCTTCCCCTTGCCTTCTTTCTCGAAAGGATGGAAAAGTTGGAGATGAGGAGCAAGGAAGGGGTGGCCAAGGTGGCAGGAACGTTAGTGGGGATTGGTGGGGCCATGTTTCTTACTTTCTACAAAGGCATCCAGATCAGCATTTGGTCAACCCACGTGGACCTCCTCCGTGGAGGACACTTGGCACATCTTCCTCAAAATCCTCACCACTCCCACAATTTTGTGCTTGGAAGTCTGCTGGCTCTCGCCAGCTGCCTCTCTTACTCTTTCTGGCTCATCCTTCAGACAAAAATGACGAAAATATATCCATGCCAATACTCGAGCACCGCGTTGATGTGTGTGATGGGAGCAATTCAGGGGTTGGCGATATCAATTTGTGTCGAGAGGGATTGGAAGCAATGGAAATTGGGTTGGAATATCAGGCTTCTCACCGTTGCATTTGCCGGGATCGTGGCGTCGGGAGCCATGGTGACGCTGATGGCGTGGTGCGTACGTATGAGAGGGCCACTGTATGTGTCTATTTTCAGCCCTCTTATGCTTCTCCTGGTAGCTATTGCTGGATCCCTATGCCTCCAAGAAAGCTTGCACCTTGGC

Protein sequence

MESGVGSSGVGSSGTTICTNGAFHATAKPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLERMEKLEMRSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLHLG

Homology

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BLAST of Carg20749 vs. NCBI nr
Match: KAG7017152.1 (WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 521.9 bits (1343), Expect = 3.2e-144
Identity = 269/269 (100.00%), Postives = 269/269 (100.00%), Query Frame = 0


Query: 1   MESGVGSSGVGSSGTTICTNGAFHATAKPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIM 60
           MESGVGSSGVGSSGTTICTNGAFHATAKPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIM
Sbjct: 1   MESGVGSSGVGSSGTTICTNGAFHATAKPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIM 60

Query: 61  IAFRFVFASLFMLPLAFFLERMEKLEMRSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIW 120
           IAFRFVFASLFMLPLAFFLERMEKLEMRSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIW
Sbjct: 61  IAFRFVFASLFMLPLAFFLERMEKLEMRSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIW 120

Query: 121 STHVDLLRGGHLAHLPQNPHHSHNFVLGSLLALASCLSYSFWLILQTKMTKIYPCQYSST 180
           STHVDLLRGGHLAHLPQNPHHSHNFVLGSLLALASCLSYSFWLILQTKMTKIYPCQYSST
Sbjct: 121 STHVDLLRGGHLAHLPQNPHHSHNFVLGSLLALASCLSYSFWLILQTKMTKIYPCQYSST 180

Query: 181 ALMCVMGAIQGLAISICVERDWKQWKLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGP 240
           ALMCVMGAIQGLAISICVERDWKQWKLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGP
Sbjct: 181 ALMCVMGAIQGLAISICVERDWKQWKLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGP 240

Query: 241 LYVSIFSPLMLLLVAIAGSLCLQESLHLG 270
           LYVSIFSPLMLLLVAIAGSLCLQESLHLG
Sbjct: 241 LYVSIFSPLMLLLVAIAGSLCLQESLHLG 269

[Close]

BLAST of Carg20749 vs. NCBI nr
Match: XP_023521464.1 (WAT1-related protein At1g25270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 432.2 bits (1110), Expect = 3.3e-117
Identity = 235/297 (79.12%), Postives = 236/297 (79.46%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER           
Sbjct: 1   MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLERNKRPKMTCSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLEMRSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVQSLAMTSATFVSAMQNLSPAITFLLALPFGMEKLEMRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           LASCLSYSFWLILQTKMTKIYPCQYSSTALMC+MGAIQGLAISICVERDWKQWKLGWNIR
Sbjct: 181 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCMMGAIQGLAISICVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. NCBI nr
Match: XP_022928857.1 (WAT1-related protein At1g25270-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-116
Identity = 235/297 (79.12%), Postives = 235/297 (79.12%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER           
Sbjct: 1   MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLERNKRPKMTCSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLE RSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVQSLAMTSATFVSAMQNLSPAITFLLALSFGMEKLETRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQN HHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNSHHSHNFVLGSLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR
Sbjct: 181 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. NCBI nr
Match: XP_022969818.1 (WAT1-related protein At1g25270-like [Cucurbita maxima])

HSP 1 Score: 430.3 bits (1105), Expect = 1.3e-116
Identity = 233/297 (78.45%), Postives = 235/297 (79.12%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFF ER           
Sbjct: 1   MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFFERNKRPKMRCSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLEMRSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVKSLAMTSATFVSAMQNLSPAITFLLALSFGMEKLEMRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKVAGTL+GIGGAMFLTFYKG+QISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLLGIGGAMFLTFYKGVQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR
Sbjct: 181 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. NCBI nr
Match: XP_023550113.1 (WAT1-related protein At1g25270-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 429.5 bits (1103), Expect = 2.2e-116
Identity = 233/297 (78.45%), Postives = 235/297 (79.12%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLA+NDGMNLMIMIAFRFVFASLFM PLAFFLER           
Sbjct: 1   MLLVQSVFAGVNVLYKLALNDGMNLMIMIAFRFVFASLFMFPLAFFLERNKRPKMTRSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLEMRSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVQSLAMTSATFVSAMQNLAPAITFLLALSFGMEKLEMRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           LASCLSYSFWLILQTKMTKIYPCQYSSTALMC+MGAIQGLAISICVERDWKQWKLGWNIR
Sbjct: 181 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCMMGAIQGLAISICVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. ExPASy Swiss-Prot
Match: Q4PT23 (WAT1-related protein At1g25270 OS=Arabidopsis thaliana OX=3702 GN=At1g25270 PE=2 SV=1)

HSP 1 Score: 206.5 bits (524), Expect = 3.9e-52
Identity = 117/302 (38.74%), Postives = 169/302 (55.96%), Query Frame = 0


Query: 28  KPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE------- 87
           K  + M+ VQ +FAG+ +L+K+ V+DG NL +++A+R  FA++FMLPLA   +       
Sbjct: 2   KSVVAMVAVQFIFAGMFILFKITVDDGTNLKVLVAYRLSFATIFMLPLALIFQRKKRPEF 61

Query: 88  -----------------------------------------------------RMEKLEM 147
                                                                RME L +
Sbjct: 62  TWRLLLLAFVSGLLGAAIPNILYLPGMARTSATFSAASSIISPLITLVLGLVFRMETLRL 121

Query: 148 RSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVL 207
            S EG AK+ GTL+G  GA+   FYKGI+I IWSTHVDLL+G H      N H S   +L
Sbjct: 122 GSNEGRAKLVGTLLGACGALVFVFYKGIEIHIWSTHVDLLKGSHTGRATTNHHVS---IL 181

Query: 208 GSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKL 267
           G L+ L S +S S WL+LQ K+ K     Y +T+LM  +G++  + I++C + DW+QW+L
Sbjct: 182 GVLMVLGSNVSTSLWLLLQAKIGKELGGLYWNTSLMNGVGSLVCVIIALCSDHDWEQWQL 241

Query: 268 GWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLH 270
           GW+I LL   ++GIV SG +V L+AWC+  +GPL+V++FSP+ L++VA+ GS  L+E LH
Sbjct: 242 GWDINLLATLYSGIVVSGMVVPLVAWCIATKGPLFVTVFSPIRLVIVALIGSFALEEPLH 300

[Close]

BLAST of Carg20749 vs. ExPASy Swiss-Prot
Match: F4HVM3 (WAT1-related protein At1g68170 OS=Arabidopsis thaliana OX=3702 GN=At1g68170 PE=3 SV=1)

HSP 1 Score: 196.1 bits (497), Expect = 5.3e-49
Identity = 109/302 (36.09%), Postives = 164/302 (54.30%), Query Frame = 0


Query: 28  KPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE------- 87
           K    M++VQ   AG+N+ +KLA+ DGMN  +++A+R +FA+LFM+P+ F  +       
Sbjct: 2   KDITAMVVVQIATAGLNIFFKLAMEDGMNPSVLVAYRLLFATLFMIPICFIFQRKKRPEF 61

Query: 88  -----------------------------------------------------RMEKLEM 147
                                                                RME + +
Sbjct: 62  TCRLMLLALLSGLLGVVIPSILTITGLALTSATFTSAAGVLTPLVTFIFAALLRMESVRL 121

Query: 148 RSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVL 207
            S  G+AKV GTL G+GGA+   FY+GI+I +WSTHV+L+     +      HH    +L
Sbjct: 122 GSSVGLAKVFGTLFGVGGALVFIFYRGIEIRLWSTHVNLVNQPRDSSRDATTHHIS--IL 181

Query: 208 GSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKL 267
           G+LL     +S S W +LQ K++K +   Y +  LM +MG +  + +++C E D  +W+L
Sbjct: 182 GALLVFGGNISISLWFLLQVKISKQFGGPYWNATLMNMMGGVVAMLVALCWEHDLDEWRL 241

Query: 268 GWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLH 270
           GWNIRLLT+A+A I+ SG +V + AWC+  RGPL+VS+FSP+ L++VA+ GS  L E+LH
Sbjct: 242 GWNIRLLTIAYAAILISGMVVAVNAWCIESRGPLFVSVFSPVGLVIVALVGSFLLDETLH 301

[Close]

BLAST of Carg20749 vs. ExPASy Swiss-Prot
Match: Q8GXB4 (WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 8.7e-36
Identity = 99/301 (32.89%), Postives = 153/301 (50.83%), Query Frame = 0


Query: 29  PALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE-------- 88
           P L M+LVQ  +AG+N+  K+A+  GM  +I++A+R +FA++   P+AFFLE        
Sbjct: 8   PFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKTRPKIT 67

Query: 89  ----------------------------------------------------RMEKLEMR 148
                                                               R E + ++
Sbjct: 68  LRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQETVGIK 127

Query: 149 SKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLG 208
              G AKV GTLV + GAM L+FY G  I I  + +      ++     +  HS NF LG
Sbjct: 128 KASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSSSGHS-NFFLG 187

Query: 209 SLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLG 268
             L +A+ +S++ W I+QTKM++ +   Y+ST LMC+MG+IQ  AI++  +     W L 
Sbjct: 188 PFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTISDWSLS 247

Query: 269 WNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLHL 270
             +R ++  +AG+VAS     LM+W ++ +GPLYVS+FSPL+L++VAI     L+E L+ 
Sbjct: 248 SPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEKLYT 307

[Close]

BLAST of Carg20749 vs. ExPASy Swiss-Prot
Match: Q9FGG3 (WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2 SV=1)

HSP 1 Score: 138.3 bits (347), Expect = 1.3e-31
Identity = 87/311 (27.97%), Postives = 147/311 (47.27%), Query Frame = 0


Query: 28  KPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER------ 87
           KP L++ ++Q ++  + ++ K   N GMN  + + +R  FA++F+ PLAFF ER      
Sbjct: 7   KPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPL 66

Query: 88  ------------------------------------------------------MEKLEM 147
                                                                 ME+L++
Sbjct: 67  SFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKV 126

Query: 148 RSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHH------ 207
           +S +G AK+ G  V +GG + L  YKG  +        L    H  H  ++PH       
Sbjct: 127 KSIQGTAKLVGITVCMGGVIILAIYKGPLLK-------LPLCPHFYHGQEHPHRNNPGHV 186

Query: 208 ---SHNFVLGSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICV 267
              S +++ G +L + S + +  WL+LQ ++ K+YP +   T L C++ +IQ   I+I +
Sbjct: 187 SGGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIAL 246

Query: 268 ERDWKQWKLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAG 270
           ERD   WKLGWN+RL+ V + G + +G    L +W +  RGP+++S+F+PL LL   ++ 
Sbjct: 247 ERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSS 306

[Close]

BLAST of Carg20749 vs. ExPASy Swiss-Prot
Match: Q9SUF1 (WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2 SV=1)

HSP 1 Score: 135.6 bits (340), Expect = 8.4e-31
Identity = 81/221 (36.65%), Postives = 126/221 (57.01%), Query Frame = 0


Query: 54  GMNLMIMIAFRFVFASLFMLPLAFF----LERMEKLEMRSKEGVAKVAGTLVGIGGAMFL 113
           GMN   M +  +  A + +LP   F    + RMEK+ +      AK+ GTLVG+GGA+ +
Sbjct: 97  GMN---MTSATYTSAIMNILPSVTFIIAWILRMEKVNIAEVRSKAKIIGTLVGLGGALVM 156

Query: 114 TFYKGIQISI-WSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLALASCLSYSFWLILQTK 173
           T YKG  I + WS      + GH      N    +N+V+G+LL L  C+++S + +LQ+ 
Sbjct: 157 TLYKGPLIPLPWSNPNMDQQNGH----TNNSQDHNNWVVGTLLILLGCVAWSGFYVLQSI 216

Query: 174 MTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIRLLTVAFAGIVASGAMV 233
             K YP   S +AL+C+ GA+Q  A+++ VER    W +GW+ RL    + GIV+SG   
Sbjct: 217 TIKTYPADLSLSALICLAGAVQSFAVALVVERHPSGWAVGWDARLFAPLYTGIVSSGITY 276

Query: 234 TLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLHLG 270
            +    ++ RGP++V+ F+PL ++LVA+  S  L E +H G
Sbjct: 277 YVQGMVMKTRGPVFVTAFNPLCMILVALIASFILHEQIHFG 310

[Close]

BLAST of Carg20749 vs. ExPASy TrEMBL
Match: A0A6J1I222 (WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111468903 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 6.1e-117
Identity = 233/297 (78.45%), Postives = 235/297 (79.12%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFF ER           
Sbjct: 1   MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFFERNKRPKMRCSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLEMRSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVKSLAMTSATFVSAMQNLSPAITFLLALSFGMEKLEMRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKVAGTL+GIGGAMFLTFYKG+QISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLLGIGGAMFLTFYKGVQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR
Sbjct: 181 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. ExPASy TrEMBL
Match: A0A6J1EL42 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435648 PE=3 SV=1)

HSP 1 Score: 430.3 bits (1105), Expect = 6.1e-117
Identity = 235/297 (79.12%), Postives = 235/297 (79.12%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER           
Sbjct: 1   MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLERNKRPKMTCSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLE RSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVQSLAMTSATFVSAMQNLSPAITFLLALSFGMEKLETRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQN HHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNSHHSHNFVLGSLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR
Sbjct: 181 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. ExPASy TrEMBL
Match: A0A1S3C690 (WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103496933 PE=3 SV=1)

HSP 1 Score: 360.9 bits (925), Expect = 4.6e-96
Identity = 195/298 (65.44%), Postives = 217/298 (72.82%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE------------ 92
           M LVQSV+AGVNVLYKLAVNDGMNLMI+IAFRF+FASLFMLPLAFFLE            
Sbjct: 1   MALVQSVYAGVNVLYKLAVNDGMNLMILIAFRFLFASLFMLPLAFFLERNKRPKMTWSFL 60

Query: 93  ------------------------------------------------RMEKLEMRSKEG 152
                                                           RMEKL+++ KEG
Sbjct: 61  FYGFFCGLFGGTLSQNLYVQSLAMTSATFVSAMQNLCPAITFLLALSFRMEKLKIKKKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPH-HSHNFVLGSLL 212
           VAKV GTL+GIGGAM LTFYKG +I+IW+THVDLL G +++HLP + H HSHN +LGS+L
Sbjct: 121 VAKVLGTLMGIGGAMILTFYKGFEINIWTTHVDLLHGRNVSHLPPHSHSHSHNLLLGSVL 180

Query: 213 ALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNI 270
           ALASCLSYSFWLILQ KM KIYPCQYSSTALMCVMGAIQG+AISIC ERDWKQWKLGWNI
Sbjct: 181 ALASCLSYSFWLILQAKMMKIYPCQYSSTALMCVMGAIQGVAISICTERDWKQWKLGWNI 240

[Close]

BLAST of Carg20749 vs. ExPASy TrEMBL
Match: A0A6J1DP24 (WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111022970 PE=3 SV=1)

HSP 1 Score: 355.1 bits (910), Expect = 2.5e-94
Identity = 191/297 (64.31%), Postives = 216/297 (72.73%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE------------ 92
           M++VQ VFAGVNVLYKLAVNDGM+L IMIAFRF+FAS+F+LPLAFF+E            
Sbjct: 1   MVVVQMVFAGVNVLYKLAVNDGMSLKIMIAFRFIFASIFILPLAFFVERNKRPKMSWSIL 60

Query: 93  ------------------------------------------------RMEKLEMRSKEG 152
                                                           RMEKL +R +EG
Sbjct: 61  LYGFFSGLFGGTLSQNLYVQSLAMTSATFVSAMQNLCPAITFLLALSFRMEKLNIRRREG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 212
           VAKV GTLVGIGGAMFLTFYKG +I IW+THVD L   H+AH P+N  H++N +LG LLA
Sbjct: 121 VAKVLGTLVGIGGAMFLTFYKGFEIRIWTTHVDFLNDRHVAHAPENA-HNYNPLLGCLLA 180

Query: 213 LASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLGWNIR 270
           +ASC+SYSFWLILQTKMT+IYPC YSSTALMCVMGAIQG+AIS+CVERDWKQWKLGWNIR
Sbjct: 181 VASCVSYSFWLILQTKMTEIYPCHYSSTALMCVMGAIQGVAISMCVERDWKQWKLGWNIR 240

[Close]

BLAST of Carg20749 vs. ExPASy TrEMBL
Match: A0A6J1EQA6 (WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435648 PE=3 SV=1)

HSP 1 Score: 316.2 bits (809), Expect = 1.3e-82
Identity = 174/236 (73.73%), Postives = 174/236 (73.73%), Query Frame = 0


Query: 33  MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER----------- 92
           MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER           
Sbjct: 1   MLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLERNKRPKMTCSIL 60

Query: 93  -------------------------------------------------MEKLEMRSKEG 152
                                                            MEKLE RSKEG
Sbjct: 61  FYGFLCGLFGGTLSQNLYVQSLAMTSATFVSAMQNLSPAITFLLALSFGMEKLETRSKEG 120

Query: 153 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLGSLLA 209
           VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQN HHSHNFVLGSLLA
Sbjct: 121 VAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNSHHSHNFVLGSLLA 180

[Close]

BLAST of Carg20749 vs. TAIR 10
Match: AT1G25270.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 206.5 bits (524), Expect = 2.8e-53
Identity = 117/302 (38.74%), Postives = 169/302 (55.96%), Query Frame = 0


Query: 28  KPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE------- 87
           K  + M+ VQ +FAG+ +L+K+ V+DG NL +++A+R  FA++FMLPLA   +       
Sbjct: 2   KSVVAMVAVQFIFAGMFILFKITVDDGTNLKVLVAYRLSFATIFMLPLALIFQRKKRPEF 61

Query: 88  -----------------------------------------------------RMEKLEM 147
                                                                RME L +
Sbjct: 62  TWRLLLLAFVSGLLGAAIPNILYLPGMARTSATFSAASSIISPLITLVLGLVFRMETLRL 121

Query: 148 RSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVL 207
            S EG AK+ GTL+G  GA+   FYKGI+I IWSTHVDLL+G H      N H S   +L
Sbjct: 122 GSNEGRAKLVGTLLGACGALVFVFYKGIEIHIWSTHVDLLKGSHTGRATTNHHVS---IL 181

Query: 208 GSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKL 267
           G L+ L S +S S WL+LQ K+ K     Y +T+LM  +G++  + I++C + DW+QW+L
Sbjct: 182 GVLMVLGSNVSTSLWLLLQAKIGKELGGLYWNTSLMNGVGSLVCVIIALCSDHDWEQWQL 241

Query: 268 GWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLH 270
           GW+I LL   ++GIV SG +V L+AWC+  +GPL+V++FSP+ L++VA+ GS  L+E LH
Sbjct: 242 GWDINLLATLYSGIVVSGMVVPLVAWCIATKGPLFVTVFSPIRLVIVALIGSFALEEPLH 300

[Close]

BLAST of Carg20749 vs. TAIR 10
Match: AT1G68170.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 196.1 bits (497), Expect = 3.7e-50
Identity = 109/302 (36.09%), Postives = 164/302 (54.30%), Query Frame = 0


Query: 28  KPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE------- 87
           K    M++VQ   AG+N+ +KLA+ DGMN  +++A+R +FA+LFM+P+ F  +       
Sbjct: 2   KDITAMVVVQIATAGLNIFFKLAMEDGMNPSVLVAYRLLFATLFMIPICFIFQRKKRPEF 61

Query: 88  -----------------------------------------------------RMEKLEM 147
                                                                RME + +
Sbjct: 62  TCRLMLLALLSGLLGVVIPSILTITGLALTSATFTSAAGVLTPLVTFIFAALLRMESVRL 121

Query: 148 RSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVL 207
            S  G+AKV GTL G+GGA+   FY+GI+I +WSTHV+L+     +      HH    +L
Sbjct: 122 GSSVGLAKVFGTLFGVGGALVFIFYRGIEIRLWSTHVNLVNQPRDSSRDATTHHIS--IL 181

Query: 208 GSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKL 267
           G+LL     +S S W +LQ K++K +   Y +  LM +MG +  + +++C E D  +W+L
Sbjct: 182 GALLVFGGNISISLWFLLQVKISKQFGGPYWNATLMNMMGGVVAMLVALCWEHDLDEWRL 241

Query: 268 GWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLH 270
           GWNIRLLT+A+A I+ SG +V + AWC+  RGPL+VS+FSP+ L++VA+ GS  L E+LH
Sbjct: 242 GWNIRLLTIAYAAILISGMVVAVNAWCIESRGPLFVSVFSPVGLVIVALVGSFLLDETLH 301

[Close]

BLAST of Carg20749 vs. TAIR 10
Match: AT1G09380.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 152.1 bits (383), Expect = 6.2e-37
Identity = 99/301 (32.89%), Postives = 153/301 (50.83%), Query Frame = 0


Query: 29  PALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLE-------- 88
           P L M+LVQ  +AG+N+  K+A+  GM  +I++A+R +FA++   P+AFFLE        
Sbjct: 8   PFLAMVLVQIGYAGMNITSKMAMEAGMKPLILVAYRQIFATIATFPVAFFLERKTRPKIT 67

Query: 89  ----------------------------------------------------RMEKLEMR 148
                                                               R E + ++
Sbjct: 68  LRILVQVFFCSITGATGNQVLYFVGLQNSSPTIACALTNLLPAVTFLLAAIFRQETVGIK 127

Query: 149 SKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFVLG 208
              G AKV GTLV + GAM L+FY G  I I  + +      ++     +  HS NF LG
Sbjct: 128 KASGQAKVIGTLVCVIGAMVLSFYHGHTIGIGESKIHWAYAENITKHGSSSGHS-NFFLG 187

Query: 209 SLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVERDWKQWKLG 268
             L +A+ +S++ W I+QTKM++ +   Y+ST LMC+MG+IQ  AI++  +     W L 
Sbjct: 188 PFLIMAAAVSWAAWFIIQTKMSETFAAPYTSTLLMCLMGSIQCGAIALISDHTISDWSLS 247

Query: 269 WNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQESLHL 270
             +R ++  +AG+VAS     LM+W ++ +GPLYVS+FSPL+L++VAI     L+E L+ 
Sbjct: 248 SPLRFISALYAGVVASALAFCLMSWAMQRKGPLYVSVFSPLLLVVVAIFSWALLEEKLYT 307

[Close]

BLAST of Carg20749 vs. TAIR 10
Match: AT2G37450.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 139.4 bits (350), Expect = 4.2e-33
Identity = 77/244 (31.56%), Postives = 143/244 (58.61%), Query Frame = 0


Query: 27  AKPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLERMEKLE 86
           A P +LM+L+Q  +AG+++L K  +N GM++ ++  +R   A++ M P AF+ +++E ++
Sbjct: 7   ALPFILMVLLQIGYAGMDILTKDVLNKGMSIYVLSVYRHGVATVVMAPFAFYFDKLESVK 66

Query: 87  MRSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHHSHNFV 146
            +S    AKV GT+  +GG M +T  KG  + ++ T     +G    +      HS   +
Sbjct: 67  FQSIRSAAKVVGTVTTVGGIMVMTLVKGPALDLFWT-----KGPSAQNTVGTDIHSS--I 126

Query: 147 LGSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICVER-DWKQW 206
            G++L    C SY+ ++ILQ    K YP + S    +C++G I+G+ +++ +E+ +   W
Sbjct: 127 KGAVLVTIGCFSYACFMILQAITLKTYPAELSLATWICLIGTIEGVVVALVMEKGNPSVW 186

Query: 207 KLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAGSLCLQES 266
            +GW+ +LLT+ ++GIV S     +    ++ RGP++V+ F PL +++VAI  S+   E 
Sbjct: 187 AIGWDTKLLTITYSGIVCSALGYYIGGVVMKTRGPVFVTAFKPLCMIVVAIMSSIIFDEQ 243

Query: 267 LHLG 270
           ++LG
Sbjct: 247 MYLG 243

[Close]

BLAST of Carg20749 vs. TAIR 10
Match: AT5G64700.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 138.3 bits (347), Expect = 9.3e-33
Identity = 87/311 (27.97%), Postives = 147/311 (47.27%), Query Frame = 0


Query: 28  KPALLMLLVQSVFAGVNVLYKLAVNDGMNLMIMIAFRFVFASLFMLPLAFFLER------ 87
           KP L++ ++Q ++  + ++ K   N GMN  + + +R  FA++F+ PLAFF ER      
Sbjct: 7   KPYLMVTIIQVIYTIMFLISKAVFNGGMNTFVFVFYRQAFATIFLAPLAFFFERKSAPPL 66

Query: 88  ------------------------------------------------------MEKLEM 147
                                                                 ME+L++
Sbjct: 67  SFVTFIKIFMLSLFGVTLSLDLNGIALSYTSATLAAATTASLPAITFFLALLFGMERLKV 126

Query: 148 RSKEGVAKVAGTLVGIGGAMFLTFYKGIQISIWSTHVDLLRGGHLAHLPQNPHH------ 207
           +S +G AK+ G  V +GG + L  YKG  +        L    H  H  ++PH       
Sbjct: 127 KSIQGTAKLVGITVCMGGVIILAIYKGPLLK-------LPLCPHFYHGQEHPHRNNPGHV 186

Query: 208 ---SHNFVLGSLLALASCLSYSFWLILQTKMTKIYPCQYSSTALMCVMGAIQGLAISICV 267
              S +++ G +L + S + +  WL+LQ ++ K+YP +   T L C++ +IQ   I+I +
Sbjct: 187 SGGSTSWLKGCVLMITSNILWGLWLVLQGRVLKVYPSKLYFTTLHCLLSSIQSFVIAIAL 246

Query: 268 ERDWKQWKLGWNIRLLTVAFAGIVASGAMVTLMAWCVRMRGPLYVSIFSPLMLLLVAIAG 270
           ERD   WKLGWN+RL+ V + G + +G    L +W +  RGP+++S+F+PL LL   ++ 
Sbjct: 247 ERDISAWKLGWNLRLVAVIYCGFIVTGVAYYLQSWVIEKRGPVFLSMFTPLSLLFTLLSS 306

The following BLAST results are available for this feature:

BLAST of Carg20749 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7017152.1	3.2e-144	100.00	WAT1-related protein, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023521464.1	3.3e-117	79.12	WAT1-related protein At1g25270-like [Cucurbita pepo subsp. pepo]	[more]
XP_022928857.1	1.3e-116	79.12	WAT1-related protein At1g25270-like isoform X1 [Cucurbita moschata]	[more]
XP_022969818.1	1.3e-116	78.45	WAT1-related protein At1g25270-like [Cucurbita maxima]	[more]
XP_023550113.1	2.2e-116	78.45	WAT1-related protein At1g25270-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of Carg20749 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q4PT23	3.9e-52	38.74	WAT1-related protein At1g25270 OS=Arabidopsis thaliana OX=3702 GN=At1g25270 PE=2...	[more]
F4HVM3	5.3e-49	36.09	WAT1-related protein At1g68170 OS=Arabidopsis thaliana OX=3702 GN=At1g68170 PE=3...	[more]
Q8GXB4	8.7e-36	32.89	WAT1-related protein At1g09380 OS=Arabidopsis thaliana OX=3702 GN=At1g09380 PE=2...	[more]
Q9FGG3	1.3e-31	27.97	WAT1-related protein At5g64700 OS=Arabidopsis thaliana OX=3702 GN=At5g64700 PE=2...	[more]
Q9SUF1	8.4e-31	36.65	WAT1-related protein At4g08290 OS=Arabidopsis thaliana OX=3702 GN=At4g08290 PE=2...	[more]

BLAST of Carg20749 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I222	6.1e-117	78.45	WAT1-related protein OS=Cucurbita maxima OX=3661 GN=LOC111468903 PE=3 SV=1	[more]
A0A6J1EL42	6.1e-117	79.12	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435648 PE=3 SV=1	[more]
A0A1S3C690	4.6e-96	65.44	WAT1-related protein OS=Cucumis melo OX=3656 GN=LOC103496933 PE=3 SV=1	[more]
A0A6J1DP24	2.5e-94	64.31	WAT1-related protein OS=Momordica charantia OX=3673 GN=LOC111022970 PE=3 SV=1	[more]
A0A6J1EQA6	1.3e-82	73.73	WAT1-related protein OS=Cucurbita moschata OX=3662 GN=LOC111435648 PE=3 SV=1	[more]

BLAST of Carg20749 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G25270.1	2.8e-53	38.74	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G68170.1	3.7e-50	36.09	nodulin MtN21 /EamA-like transporter family protein	[more]
AT1G09380.1	6.2e-37	32.89	nodulin MtN21 /EamA-like transporter family protein	[more]
AT2G37450.1	4.2e-33	31.56	nodulin MtN21 /EamA-like transporter family protein	[more]
AT5G64700.1	9.3e-33	27.97	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 147..268 e-value: 1.7E-7 score: 31.5
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 28..81 coord: 82..269
None	No IPR available	PANTHER	PTHR31218:SF195	WAT1-RELATED PROTEIN	coord: 28..81 coord: 82..269

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20749-RA	Carg20749-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity