Carg20714 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20714
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptionpullulanase 1, chloroplastic
LocationCarg_Chr18: 9345056 .. 9353321 (+)
RNA-Seq ExpressionCarg20714
SyntenyCarg20714
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTGTTTAACACTCACTGTCTACATAAGACATGTTACAGATATCGAACTTCTGGAAAAACTTCCTCCAGATTCAGCTGAACAACAATCATTAATTGCAGATATCCAAAACAGCGATGGGTATAACTGGGGGTAAGCTCTTGATTTTGGAGTCTTCTTTAAAGCTGTTAGGTTCTTTGAGTTTTATGGACTCATTTGTGTATGTTTGTTGTGTCTTTTTAGGTATAATCCTATTCTGTGGGGAGTACCTAAAGGAAGCTATGCTAGTGATCCTAATGGTCCTTGTCGTTTAATTGAGTTCAGAAAGATGGTTCAGGTTGACAAACTTGTTTATGTTTCTAACAAGTCTAAATCAATATATATTCAGTTATCAATTGCAAGTAGTGCAATCTATCATTTTAAAGTATTCATTTATTACAGGCACTTAACCAAATTGGCCTTCGTGTTGTGTTGGATGTTGTCTATAATCATCTACATGGATGTGGACCTTTTGATCCAAGTTCTGTTCTTGATAAGGTCTCAACCTCTAATTGTTTTTGATGCATTCTTGTGCTTTCAATATTGCTCAACCCACCACTAAAGATTTCGTTGTTCATGTCTTACCTCTTCATGTTAACATTTTCTCGATGAGGGTTTCTGTTTTTCTTTATGATATCGTTTTCCTCTCTGGTAGATTGTACCGGGTTACTATTTGAGAAGGAACATTGATGGTTTCATTGAGAACAGTACCTGTGTAAATAACACTGCCAGTGAGCATTTTATGGTTGAACGCATGATTGTGGATGATATCTTACATTGGGTAGTTGATTATAAGGTATTTACATACAAAAATATTGAATCTTCAGCTCCTTTTGTGCTGTTGCATGCAGACAGAACTTGACCTTTATATCTGTCGAACACAATTTCTTGATGTTTCCCAGGTTGATGGATTTCGGTTTGATCTTATGGGGCATTTGATGACAAGTACTATGGTATGTGACTTTATTTTCACTTAAATTCATACATAGTACTTATAGAGCTTCATAATTCAATTGCAATACTCGATATTTATTATCTGATTCTAATGTTGCAATGGCTATTGAGCCCACTGTACATGAACTTTGAAACTAAGACTAAACTTCTGGTCTGCATGAAATGTATGGTTACTTGAAGTTGAAAGCAAAAAACGCACTGCAAATATTAACACAGGAAAAGAATGGAGTTGATGGTTCAAGTATCTACCTGTAAGTTTGGGCAAATTTCCTTACTGTTGCACATTGTGTTTGTAGCATAAGTTAATTCCAATGTGAGATTTGAAGATTCTTTTGCATTCTCACACATTTCAATTTAGATATGGTGAAGGATGGGACTTCGGCGAAGTTGCAAAAAATGGTCGTGGAGTAAATGCATCACAGTTCAATCTTTTTGGAACTGGAATTGGGAGGTATATACTTTTTTTTCTTCTGCAAGTTTTGTGATCAGGCAATAAATGTTTCAATTGCAAATCTGCTAAGCAATCCTCGAGATGGACTAACTAGTGGCTGCTCTTGGCAATTTTATTGAAAAATGCAAAATCGCATTATTTTTCCCATTTTATGTTCTAAGAGATCTTAAAGATGGATACTTTTCTACCCTATGGATCTTGCGCCTTTGTTAATTTTCTTGGTTGATTGAATGAATATAAACACCTCATTTTGATGTCTGCGTTATTTGGTCTTAATTTATAAATACAACTGTTCGCTAGTTAGAAAACGTAATTTCCCTATTAGCTTGGCACGTGTTTCTTTTATGCACGAGTTCGAATATAAACACGTTGTGATTTCCCTATAAGCGTTGTGTTTGCTTATGGCACATGTTTCTTTTATGCACGAGTGATTTCCCTTTCGGATTGGTTTTCAGTGTTGTGTTTGCTTATGGAAAAGTAATATTGGGAAATTTTAAATTTATCGTGTTTTTTCAGTTTTAATGATCGAGTTCGAGATGCAATGCTTGGTGGATCTCCATTTGGTCATCCTCTTCAGCAAGGATTTGTGACTGGTCTATTGCTCGAGGTATTTTGGAATTTCTTTGATCCGGGCGTCCTATAATCTGATCTTGACAGTTTGTCATCGTGTTCAATCAAATATCACAATGGAGGAAAATTGCTCCGGTTACTATTGATTTAGCAGGTTTTAGGTGTAAGACACGGTTTTCACTCTGGTCTAGAGAAGTGGGATTTACTTGGAACGAAAAATTTCCTGTTCAGATCATTATTTAAGAAAAAGCGAAGTGTCTATTCATCGAATTTTTATTGAATTGTAGGTTTTTCTTCCGTTGAATTTAGGATAAACTACGAGAGCAGATAGTATCTAGAACTTCCCACCTCCCTAACTCTTTTGGCTAGCCTTATTCTTTAGAGAGAAGAGATTGGTTAATGTTCAAGCCTTCATTGTCTCCGTTCATCCAATCTTTATACTTGAAAGAGAAGAAATTGGTTAAACCAAAAAATGCGGCCAGCAACTTCTAAACACCAACCACACCCTAGAAAAATAGAAGTTTCAAACACTCCCCATAAGCAAACACAACGCTGAAAACCAATCCGAAAGCACATTGCTGGCAACTTTTATCAATTCCAAAATACCCCAATATTCTTAGGACTCTGGCTTTGAGACCATGTTAAATCCCTAATCAAACCAAAAACTTAAGCTGATGGATCATGGTAGATTTAACTATATCAACACTATAACACTTTCCCTCCAACAAGTGGAAATCAATATTAATTGGGAAGGAAATGATTTTTCAGGGGTTCAAACAAAAGACCTCTTTCTCTTATACTATGTCTAATTAAGAAAGAGAAGTTTGTTGATGAAATATTATGTCACGTAATGCTTTCCTCATTTGAATGTAATTTTTTTCTATATTTTTTTGTTTATAAATTTGTTTGTTAAATAATTTGTATGTTGAATTAAAAGCGTTTTGTATTTTTCTCTGGCTGTATCACTAACATTTGTACTTCATTTAATTACAGAGTTTTCTTCCTTGTGTCCAATGTATTCAATTTTTCTTATTTTGCAGCCTAATGATCATGATCATGGTACAGACAAAGATGCGGAATTCACGCTTGCTGTGTCAAAGGATCATATTCAGGTTAGTCACCTCTGTGTGAGTTGAAGTGGCCCACTCTTAGAATCAGTCTTCGTATGTTCTGTTAGTAGCCCATGTGTTGTAGGAAATTTTTTATAATTGCAATTATTATAATTTTTTTAATATAGTGAATGTCCAAGCCTGTTTGCACGCATATTGACTATTCTCACGGGACAACCATTTGGTTGCCAAGAGAACTCATAGGATATGAGATTCTAGGTAGGTAACCATAATAACTTGAACATGAGGCATGTACTATCTTGATTAATTGGAGATGAACTATGTTTATGTAGCCAATACTGGTTGAATTTACATATAGATAAAAGAAGAGTAATTCACCTTTCTTACAATATATAAGGAAGTGAGAATCGACTATCAAGCACTAACTTACTTGAGTTGAAACATGAAAAACGTTGGTTGGAGTTGAGATGGGTTAGGGATAATAGGCTCATGAACCTTGCCATTAACAAATAGTTGGAAGAAGGAGGAGAAATTGTAAACTTGGAGGGATGAACGGGGAGAGAGAGAAGTAGAAAATTTGAACAGGCTGAAGCAGTCGTGAGTCGTCTAAGAGAAGTAGAGAAAGAAGAGGACCTTTGAACGAGGTGGGAGAAAGTTTTTTTGTGAGGACAAGGACTCAACAAAAAAGGAGAAATGGAGGGAGAAGGAATAGAGGGTGGATGACATGATTTGACGTTGCAGACCCAGAAAAATTCACACTGGACTCGCCTTATTCTATTTTTTCAGCAATTCAAACGTCTATAGCTACTTATTTTAGGACATCCTAACTCAGCAAAATTTTGGTAAAACTCTCATTTTTTGTGCTGAAAGAACATAAAAGAACTCTGGCTTATTCTAGAAAGACTGCATTTTACAAGTCATTAATATAAAGAAAGAATTGGAAAAATGCTAGGAAAAATTTGAAAAGAGAAATCTGGTTTTGAATTGAGAATATTGATTATTTCAACATTTGCATTGTACAAAGCCATCAATATAGTCAGATTTATGTTTAGCAATATAGCTACTCCAGTATATTATTACTTCCCTCGGGTTCCGATCCAATGTATTTTAACTACTTACAATAAATTTTAGGTTGCCATGGCTGCCAATTTGAAGGACTACGTTCTAGCCAATTTTGAAGGAAAGGAGGTATTTTCCTTGAAGCAAGCAATTTCTCATTAGGAACATTTTAAGAGGGATTTGGTATTTGTTTTCTAACATGTTTGTGTGTGTGTGTGTATGCATGCAAAGATACGATTGATTTCACTGACTTTATCTAGTTCATTTGCCTGTGTTTTCCTCGTTTTGAATGAAAAACTTTACGGTAAAAAGTTGTGAAAGGACATAGTTATTGATTTTCCACATCTATGATAATATACCGTTTGTCTACTATCCTTTTTCAATTAAAACAAGTTCTTTTCCTTTTACACAAAAAATATAGTTTTTAAATAATATAGACGTATACTTCCTTATGGGGGCTGGGGGTTATTTCCATGAAAAGGATATGTTGTTAAATCAATCGTTATTCCTGCATTCTGATTTACATGATGTAAAATATTTTAGTATACTTGTCTTAACGAATACAAATGGAACTGGTTCATTTTTCTGCACCTGCGTATCATTAGTCTTTTCCAACAAGATTTGCATTTCATTTTTTCATGAAGAATTTTAGAAATATCATATCTTTTTCCAGGTGAAAGGATCAGAAGTACGGACTTATGATGGATCTTCAGTGGCGTATGCTTCTTGCCCCACAGAAACTGTAAGATGCACCGTCATTTTGGGAACCGCCGTTATCATTTATCTAGGCTCACATTACCTCGGATGTTAAAATAATAGTTAAGTTCTGATATGCACAGCCATGAATTTGACATTCTCGATTGATTCCCAGGTGAACTATGTTTCCGCTCATGATAATGAAACCCTGTTTGACGTTGTGAGTTTGAAGGTATTGTGAATGTACTTTTGTGAATCATACTGATTTCTTTAAGAGTTGACGTCTGATTATAGTCTTTTTCTTGTAGACTCCGAGAAACATTACCGTGAATGACAGGTGCAGGATGAATCATTTGGCAACCAGTATTATTGCACTATCGCAGGTATTTAATTTTAATTAATCATGAAAATTCAAGGCATTACATGTGCAAATCAGAAGAAACTTATTTCACACAAAACCATGTTCATGTGCCTCAATAAATTACCAGCAATTCCGGAAGCTGAGGTTATTCAGATATGGGCTATATATAGCTACAGGAATCCTATTCTTCTGAACATTTAACATCCCTAGTCAATGTCCTGCCCTTACCGACATGAGTCTGCAGATGACACTAAACGTGGTCTTGTTCTTTATAGAACCAAAAGATTAAACTAGATAAGGAAATTGAAACGGGAAGGTAGCTAGATCAGAACCAAGGAACTCATACAATATTCATAACTTTAACCAAGTCGCTGGTGATTTGGGACGTGGATGCCAGATATGTTTTTGGAGTTCTTTATAACTGTGTGGACTGTGACATCTAGATCTGAACAATGAAAACCTTGTTTTCTATTTATCGTTTGATAAATTTTATGTGCATTGACATAATTATACACTATCGAGTAACTGACTGAATTAGTTAATTATGATTCGTAGTTTTGTAATTCTTGTCACTGAATCACTCTCTTCCTCTTTAAGGGAATACCATTTTTCCACTGTGGTGATGAATTACTACGCTCAAAATCAATGGATCGTGATTCATACAACTCTGGTGATTGGTTCAACAGGTCCTGTTCTTTTTCCTTTTTTTTTGCATTCAATTATTTATTAACATTTCGTAAGTATTTAGATGTTGAGGGCACCGACTACAGTCTTTAAAAAAAAAAAAAAATTGTTCTAGTTTTCTGCAAATTTTATGCAGTACATAATTTGGAACATATGATATATTACACATTGGGTATCAAATCTCATTTTCTCTAACTTAAATGGTATACTGCCAGTTATGGTTTGTCTTTGTTTACCTTGAATTGATTTTTACTTTTCTCAAAATCAGACTCGACTTCACTTACATGACAAATAATTGGGGCGTTGGCCTTCCTCCAAAAGAGAAGAATGAATACAATTGGCCTCTGTAAGTTTTAAGAAATTTGCTAGATTTTGAGTAGCATTCCTTCTGCAAGTCTTCCTTCCCCTTCTGGAATCTGGACTAGATGGAACTTATTCCTATTCTTAAAAAATTTGTGGGAGGTTTACTGGAAAGCTTTGTTAATAATGTCATCCATTTTCAGAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTGGAAAATTTTACAAATCTGTTACAAATCAGATACTCTTCACCACTTTTTCGTTTGAAGACAGCTAATGCTATCCAGGTAAGAATGCATGTTTAAGAATAATTGTCAGATGAAATATAATGTAAATTTTTAATTCAGCTGATTAATCTTGGGGACTTGAGATCCATATTCAATAAAACGGTCTTTTTCTTGTACAAGAGCAACATAATACAGGGCTCAAAGCATCATTACTTTGTTTCAGGCTGGTCCACAATTTACACTGCTTTATTCCAATCTCTACTTTTCGACATGCAAAATTGAAATGTTATGGTTGAACCAGTTAAACTGGACATAACGTAGCACCAGCTATTCAAACAGATATCAGTTAGCCTGCTTGTTTAAAGGCTTAGATGTATGACATCATTAGCTCCGTTTCCCTAGAATTGCTACACATGTCTGGTTTGGATTGGAATGGAATTTACTTTTCTGGTGCTTGTGATGTGAAGTGCCTTTGCCTTTTCTTGTGTAGAAACGGGTCCGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAAGGCATTCCTGGGTTAACTCAGCTGGATCCCATGTGAGTAGAATAGCTTTCTCTAAATCCATGATTTTACTCATAATAATATTGTTATCTCATGTTTTCTGCAGTTACTCCTACATTGTTGTTGTGGTTAATGCACGCCCATCTGAGATTTCATTTCCTTGTCCTGCATTGCGCGCAAGAACTCTCCAATTACATCCCATTCAGGTAAATAAATACTCTCTTGAGATCCAACACTGCTTCGCTCCAAAAGAGAACTTTTGCTAACTATAGCTCTTATCTTTATTATTTGTGCTATGTCAACGTTTTAAATATGGCAGTTGATGTCAACCGATCCAATGGTTAAAAATTCAGCTTACGAGCCATCCACAGGATGCTTCATGGTGCCCTCAAGAACCACATCAGTATTTGTGGAGCTACGGAAGCATGAGTAGCCTCATCCTCCGGAATGGTACTCCCTTATTCTTATTCCATTTCAAAATGTGAGAATTACACCATTTTTGGTGTATTCCTCATATTCATCATAGCACTATATGTTAAATCAAAAAGTCTAAGTTCATAGATACTTTTTTAATATATATGTCACAAGAAACTCAATAAGTGTCCTGCCAATACTATAAAAAGACAATCACAATAAGAGTTGGCATACAAGTCCTCTTACTCTAATGTTATGTCACATCATAAAATTTATTAATTTGATTCTTTTCTCTCCATAGTCCTTTGCAATTTGGTGTTTAGTATAATCTATAATATGAGGTAGTGCTTTTGATTTCTCTTCTCCAATGAAAAATCCAAGTTGAGAAGGACTATTATCGTTGGTCAATACTCCCAATACAAAATATCTTGGACTTAATCTAGCTTTCTCCATCAGGTGATGTTGTGACTGCCATTTCTTTTTGCCACCCTTCTACTCACACCCACCTCCCCAAGGGGGAAAATGGGCATGAATAATCAATCCTTCATACCCTTCTCTTTTATTATTACTCCAATAATGTCAATGATAGGGACTGAACCTTCGATTTTATTTTCAAATGGGCCCTACTTCTATGTGGATGGACAGTGGAACTGAAAGTGGAGGCTTTTGTTGAAGGGCTTTATGATCTCTTTCTTTTATTTGGATAATATACATACTCACTTTCTTAGTGCTTTTCTAAGGAGAAAATTCCACTTCTGAGGTAATATGTTGAGTTGAGTTTTGGTATTGATAAGTGTATGACCATTATTCTTCCATCTTCCTTGGGGTTAAAGTTAAAAGAAA

mRNA sequence

ATGTGTTTAACACTCACTGTCTACATAAGACATGTTACAGATATCGAACTTCTGGAAAAACTTCCTCCAGATTCAGCTGAACAACAATCATTAATTGCAGATATCCAAAACAGCGATGGGTATAACTGGGGGTATAATCCTATTCTGTGGGGAGTACCTAAAGGAAGCTATGCTAGTGATCCTAATGGTCCTTGTCGTTTAATTGAGTTCAGAAAGATGGTTCAGGCACTTAACCAAATTGGCCTTCGTGTTGTGTTGGATGTTGTCTATAATCATCTACATGGATGTGGACCTTTTGATCCAAGTTCTGTTCTTGATAAGATTGTACCGGGTTACTATTTGAGAAGGAACATTGATGGTTTCATTGAGAACAGTACCTGTGTAAATAACACTGCCAGTGAGCATTTTATGGTTGAACGCATGATTGTGGATGATATCTTACATTGGGTAGTTGATTATAAGGTTGATGGATTTCGGTTTGATCTTATGGGGCATTTGATGACAAGTACTATGTTGAAAGCAAAAAACGCACTGCAAATATTAACACAGGAAAAGAATGGAGTTGATGGTTCAAGTATCTACCTATATGGTGAAGGATGGGACTTCGGCGAAGTTGCAAAAAATGGTCGTGGAGTAAATGCATCACAGTTCAATCTTTTTGGAACTGGAATTGGGAGTTTTAATGATCGAGTTCGAGATGCAATGCTTGGTGGATCTCCATTTGGTCATCCTCTTCAGCAAGGATTTGTGACTGGTCTATTGCTCGAGCCTAATGATCATGATCATGGTACAGACAAAGATGCGGAATTCACGCTTGCTGTGTCAAAGGATCATATTCAGGTTGCCATGGCTGCCAATTTGAAGGACTACGTTCTAGCCAATTTTGAAGGAAAGGAGGTGAAAGGATCAGAAGTACGGACTTATGATGGATCTTCAGTGGCGTATGCTTCTTGCCCCACAGAAACTGTGAACTATGTTTCCGCTCATGATAATGAAACCCTGTTTGACGTTGTGAGTTTGAAGACTCCGAGAAACATTACCGTGAATGACAGGTGCAGGATGAATCATTTGGCAACCAGTATTATTGCACTATCGCAGGGAATACCATTTTTCCACTGTGGTGATGAATTACTACGCTCAAAATCAATGGATCGTGATTCATACAACTCTGGTGATTGGTTCAACAGACTCGACTTCACTTACATGACAAATAATTGGGGCGTTGGCCTTCCTCCAAAAGAGAAGAATGAATACAATTGGCCTCTAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTGGAAAATTTTACAAATCTGTTACAAATCAGATACTCTTCACCACTTTTTCGTTTGAAGACAGCTAATGCTATCCAGAAACGGGTCCGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAAGGCATTCCTGGGTTAACTCAGCTGGATCCCATTTACTCCTACATTGTTGTTGTGGTTAATGCACGCCCATCTGAGATTTCATTTCCTTGTCCTGCATTGCGCGCAAGAACTCTCCAATTACATCCCATTCAGTTGATGTCAACCGATCCAATGGTTAAAAATTCAGCTTACGAGCCATCCACAGGATGCTTCATGGTGCCCTCAAGAACCACATCAGTATTTGTGGAGCTACGGAAGCATGAGTAGCCTCATCCTCCGGAATGGTACTCCCTTATTCTTATTCCATTTCAAAATGTGAGAATTACACCATTTTTGGTGTATTCCTCATATTCATCATAGCACTATATGTTAAATCAAAAAGTCTAAGTTCATAGATACTTTTTTAATATATATGTCACAAGAAACTCAATAAGTGTCCTGCCAATACTATAAAAAGACAATCACAATAAGAGTTGGCATACAAGTCCTCTTACTCTAATGTTATGTCACATCATAAAATTTATTAATTTGATTCTTTTCTCTCCATAGTCCTTTGCAATTTGGTGTTTAGTATAATCTATAATATGAGGTAGTGCTTTTGATTTCTCTTCTCCAATGAAAAATCCAAGTTGAGAAGGACTATTATCGTTGGTCAATACTCCCAATACAAAATATCTTGGACTTAATCTAGCTTTCTCCATCAGGTGATGTTGTGACTGCCATTTCTTTTTGCCACCCTTCTACTCACACCCACCTCCCCAAGGGGGAAAATGGGCATGAATAATCAATCCTTCATACCCTTCTCTTTTATTATTACTCCAATAATGTCAATGATAGGGACTGAACCTTCGATTTTATTTTCAAATGGGCCCTACTTCTATGTGGATGGACAGTGGAACTGAAAGTGGAGGCTTTTGTTGAAGGGCTTTATGATCTCTTTCTTTTATTTGGATAATATACATACTCACTTTCTTAGTGCTTTTCTAAGGAGAAAATTCCACTTCTGAGGTAATATGTTGAGTTGAGTTTTGGTATTGATAAGTGTATGACCATTATTCTTCCATCTTCCTTGGGGTTAAAGTTAAAAGAAA

Coding sequence (CDS)

ATGTGTTTAACACTCACTGTCTACATAAGACATGTTACAGATATCGAACTTCTGGAAAAACTTCCTCCAGATTCAGCTGAACAACAATCATTAATTGCAGATATCCAAAACAGCGATGGGTATAACTGGGGGTATAATCCTATTCTGTGGGGAGTACCTAAAGGAAGCTATGCTAGTGATCCTAATGGTCCTTGTCGTTTAATTGAGTTCAGAAAGATGGTTCAGGCACTTAACCAAATTGGCCTTCGTGTTGTGTTGGATGTTGTCTATAATCATCTACATGGATGTGGACCTTTTGATCCAAGTTCTGTTCTTGATAAGATTGTACCGGGTTACTATTTGAGAAGGAACATTGATGGTTTCATTGAGAACAGTACCTGTGTAAATAACACTGCCAGTGAGCATTTTATGGTTGAACGCATGATTGTGGATGATATCTTACATTGGGTAGTTGATTATAAGGTTGATGGATTTCGGTTTGATCTTATGGGGCATTTGATGACAAGTACTATGTTGAAAGCAAAAAACGCACTGCAAATATTAACACAGGAAAAGAATGGAGTTGATGGTTCAAGTATCTACCTATATGGTGAAGGATGGGACTTCGGCGAAGTTGCAAAAAATGGTCGTGGAGTAAATGCATCACAGTTCAATCTTTTTGGAACTGGAATTGGGAGTTTTAATGATCGAGTTCGAGATGCAATGCTTGGTGGATCTCCATTTGGTCATCCTCTTCAGCAAGGATTTGTGACTGGTCTATTGCTCGAGCCTAATGATCATGATCATGGTACAGACAAAGATGCGGAATTCACGCTTGCTGTGTCAAAGGATCATATTCAGGTTGCCATGGCTGCCAATTTGAAGGACTACGTTCTAGCCAATTTTGAAGGAAAGGAGGTGAAAGGATCAGAAGTACGGACTTATGATGGATCTTCAGTGGCGTATGCTTCTTGCCCCACAGAAACTGTGAACTATGTTTCCGCTCATGATAATGAAACCCTGTTTGACGTTGTGAGTTTGAAGACTCCGAGAAACATTACCGTGAATGACAGGTGCAGGATGAATCATTTGGCAACCAGTATTATTGCACTATCGCAGGGAATACCATTTTTCCACTGTGGTGATGAATTACTACGCTCAAAATCAATGGATCGTGATTCATACAACTCTGGTGATTGGTTCAACAGACTCGACTTCACTTACATGACAAATAATTGGGGCGTTGGCCTTCCTCCAAAAGAGAAGAATGAATACAATTGGCCTCTAATCAAACCAAGACTGGCCGATCCCTCCTTCAAACCTAGTAAAAGTCACATTCTTGCTGCTGTGGAAAATTTTACAAATCTGTTACAAATCAGATACTCTTCACCACTTTTTCGTTTGAAGACAGCTAATGCTATCCAGAAACGGGTCCGTTTCCACAATACAGGCACGTCGCTTATACCTGGAGTCATAGTGATGAGCATTGAAGATGGGCAGGAAGGCATTCCTGGGTTAACTCAGCTGGATCCCATTTACTCCTACATTGTTGTTGTGGTTAATGCACGCCCATCTGAGATTTCATTTCCTTGTCCTGCATTGCGCGCAAGAACTCTCCAATTACATCCCATTCAGTTGATGTCAACCGATCCAATGGTTAAAAATTCAGCTTACGAGCCATCCACAGGATGCTTCATGGTGCCCTCAAGAACCACATCAGTATTTGTGGAGCTACGGAAGCATGAGTAG

Protein sequence

MCLTLTVYIRHVTDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGCFMVPSRTTSVFVELRKHE
Homology
[Close]
BLAST of Carg20714 vs. NCBI nr
Match: KAG7012918.1 (Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1185.6 bits (3066), Expect = 0.0e+00
Identity = 575/575 (100.00%), Postives = 575/575 (100.00%), Query Frame = 0 

Query: 1   MCLTLTVYIRHVTDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASD 60
           MCLTLTVYIRHVTDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASD
Sbjct: 1   MCLTLTVYIRHVTDIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASD 60

Query: 61  PNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDG 120
           PNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDG
Sbjct: 61  PNGPCRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDG 120

Query: 121 FIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQI 180
           FIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQI
Sbjct: 121 FIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQI 180

Query: 181 LTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSP 240
           LTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSP
Sbjct: 181 LTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSP 240

Query: 241 FGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEV 300
           FGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEV
Sbjct: 241 FGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEV 300

Query: 301 KGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATS 360
           KGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATS
Sbjct: 301 KGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATS 360

Query: 361 IIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNW 420
           IIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNW
Sbjct: 361 IIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNW 420

Query: 421 PLIKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSL 480
           PLIKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSL
Sbjct: 421 PLIKPRLADPSFKPSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSL 480

Query: 481 IPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLM 540
           IPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLM
Sbjct: 481 IPGVIVMSIEDGQEGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLM 540

Query: 541 STDPMVKNSAYEPSTGCFMVPSRTTSVFVELRKHE 576
           STDPMVKNSAYEPSTGCFMVPSRTTSVFVELRKHE
Sbjct: 541 STDPMVKNSAYEPSTGCFMVPSRTTSVFVELRKHE 575

[Close]
BLAST of Carg20714 vs. NCBI nr
Match: XP_022945721.1 (pullulanase 1, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM
Sbjct: 405 DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS
Sbjct: 465 VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI
Sbjct: 525 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL
Sbjct: 585 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV
Sbjct: 645 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC
Sbjct: 705 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
           EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP
Sbjct: 885 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 944

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVPSRTTSVFVELRKHE
Sbjct: 945 STGCFMVPSRTTSVFVELRKHE 966

[Close]
BLAST of Carg20714 vs. NCBI nr
Match: KAG6573853.1 (Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1157.9 bits (2994), Expect = 0.0e+00
Identity = 561/562 (99.82%), Postives = 561/562 (99.82%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM
Sbjct: 405 DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHGCGPFDP SVLDKIVPGYYLRRNIDGFIENSTCVNNTAS
Sbjct: 465 VQALNQIGLRVVLDVVYNHLHGCGPFDPISVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI
Sbjct: 525 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL
Sbjct: 585 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV
Sbjct: 645 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC
Sbjct: 705 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
           EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP
Sbjct: 885 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 944

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVPSRTTSVFVELRKHE
Sbjct: 945 STGCFMVPSRTTSVFVELRKHE 966

[Close]
BLAST of Carg20714 vs. NCBI nr
Match: XP_031741579.1 (pullulanase 1, chloroplastic isoform X2 [Cucumis sativus])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.7e-308
Identity = 513/562 (91.28%), Postives = 537/562 (95.55%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D ELLE LPPDSAEQQSLIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKM
Sbjct: 272 DTELLENLPPDSAEQQSLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKM 331

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 332 VQALNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTAS 391

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+AL+ LT+EKNGVDGSSI
Sbjct: 392 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSI 451

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           Y+YGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGL
Sbjct: 452 YIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGL 511

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGT + AE  LAVSKDHI+VAMAANL+DY+L NFEGKEVKG EV T+D S V
Sbjct: 512 LLEPNDHDHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPV 571

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFD+VSLKTPRNITV+DRCR+NHLATSIIALSQGIPFFHC
Sbjct: 572 AYASCPTETVNYVSAHDNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHC 631

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 632 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 691

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKT+NAIQKRVRFHN+GTSLIPG+IVMSIEDG 
Sbjct: 692 PSKSHILAAVENFTNLLQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGH 751

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
            GIPGL+QLD  YSYIVVVVNARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEP
Sbjct: 752 NGIPGLSQLDSTYSYIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEP 811

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVP RTTSVFVE R HE
Sbjct: 812 STGCFMVPPRTTSVFVEPRMHE 833

[Close]
BLAST of Carg20714 vs. NCBI nr
Match: KAE8648841.1 (hypothetical protein Csa_008972 [Cucumis sativus])

HSP 1 Score: 1067.0 bits (2758), Expect = 5.7e-308
Identity = 513/562 (91.28%), Postives = 537/562 (95.55%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D ELLE LPPDSAEQQSLIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKM
Sbjct: 336 DTELLENLPPDSAEQQSLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKM 395

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 396 VQALNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTAS 455

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+AL+ LT+EKNGVDGSSI
Sbjct: 456 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSI 515

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           Y+YGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGL
Sbjct: 516 YIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGL 575

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGT + AE  LAVSKDHI+VAMAANL+DY+L NFEGKEVKG EV T+D S V
Sbjct: 576 LLEPNDHDHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPV 635

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFD+VSLKTPRNITV+DRCR+NHLATSIIALSQGIPFFHC
Sbjct: 636 AYASCPTETVNYVSAHDNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHC 695

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 696 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 755

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKT+NAIQKRVRFHN+GTSLIPG+IVMSIEDG 
Sbjct: 756 PSKSHILAAVENFTNLLQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGH 815

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
            GIPGL+QLD  YSYIVVVVNARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEP
Sbjct: 816 NGIPGLSQLDSTYSYIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEP 875

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVP RTTSVFVE R HE
Sbjct: 876 STGCFMVPPRTTSVFVEPRMHE 897

[Close]
BLAST of Carg20714 vs. ExPASy Swiss-Prot
Match: Q8GTR4 (Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2)

HSP 1 Score: 887.1 bits (2291), Expect = 1.1e-256
Identity = 415/559 (74.24%), Postives = 480/559 (85.87%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D  LLE L PDS E Q+ I +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKM
Sbjct: 405 DTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALN  GL VVLDVVYNHLH  GP D  SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 465 VQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EH+MV+R+I DD+L+WVV+YKVDGFRFDLMGH+M +T++ AK+A+  L +E +GVDGS I
Sbjct: 525 EHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           YLYGEGW+FGEVA+NGRG+NASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGL
Sbjct: 585 YLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LL+PN HDHG++   E  L+ +K+HIQ  MAANLKDY+L N EGKEVKGSEV  +D + V
Sbjct: 645 LLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYAS PTET+NYVSAHDNETLFD++SLKTP  I+V++RCR+NHLA+S+IALSQGIPFFH 
Sbjct: 705 AYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHA 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDE+LRSKS+DRDSYNSGDWFNRLDF+Y +NNWGVGLPPK KNE+NWPLIKPRL DPSFK
Sbjct: 765 GDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           P  SHI+A + NF +LL+IRYSSPLFRL TA AIQ+RVRFHNTG S IPG IVMSIEDG 
Sbjct: 825 PKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGH 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
            GIP ++Q+DPIYS IVV+ NARPSE S+P PAL+ R L+LHP+Q+MS D +VK S Y+ 
Sbjct: 885 RGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDS 944

Query: 554 STGCFMVPSRTTSVFVELR 573
            +G F VP+RTT+VFVE R
Sbjct: 945 FSGGFTVPARTTTVFVESR 963

[Close]
BLAST of Carg20714 vs. ExPASy Swiss-Prot
Match: P07206 (Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2)

HSP 1 Score: 385.6 bits (989), Expect = 1.0e-105
Identity = 219/550 (39.82%), Postives = 322/550 (58.55%), Query Frame = 0 

Query: 24   DSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQ-IGL 83
            D+ + Q+L   +  +D YNWGY+P  + VP+GSYA+DP G  R+ EFR M+QA+ Q +G+
Sbjct: 545  DNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTTRIKEFRTMIQAIKQDLGM 604

Query: 84   RVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRN-IDGFIENSTCVNNTASEHFMVERM 143
             V++DVVYNH +  GP D +SVLDKIVP YY R N   G +E++TC +++A EH M  ++
Sbjct: 605  NVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDSAPEHRMFAKL 664

Query: 144  IVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWD 203
            I D +  W  DYK+DGFRFDLMG+   + +L A   ++ L  +        IY +GEGWD
Sbjct: 665  IADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPD--------IYFFGEGWD 724

Query: 204  FGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPF--GHPLQ--QGFVTGLLLEP 263
              +   + R   ASQ NL GTGIG+F+DR+RD++ GG PF  G  L+  QG  +G  + P
Sbjct: 725  SNQ---SDRFEIASQINLKGTGIGTFSDRLRDSVRGGGPFDSGDALRQNQGIGSGAGVLP 784

Query: 264  NDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYAS 323
            N+    +D           D  ++ MA NL D+V+ + +G   KGSE+  Y+G+   YA+
Sbjct: 785  NELASLSDDQVRHL----ADLTRLGMAGNLADFVMIDKDGAAKKGSEI-DYNGAPGGYAA 844

Query: 324  CPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDEL 383
             PTE VNYVS HDN+TL+D++S K  +   +  R RM  ++ + + L QGI F   G EL
Sbjct: 845  DPTEVVNYVSKHDNQTLWDMISYKASQEADLATRVRMQAVSLATVMLGQGIAFDQQGSEL 904

Query: 384  LRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKS 443
            LRSKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+ +    P ++
Sbjct: 905  LRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRISDDGSNYEVI-TRVKEMVATPGEA 964

Query: 444  HILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQEGIP 503
             +      +  L ++R SSPLF L   +A+ KRV F NTG+    G++VM+++DG +   
Sbjct: 965  ELKQMTAFYQELTELRKSSPLFTLGDGSAVMKRVDFRNTGSDQQAGLLVMTVDDGMK--- 1024

Query: 504  GLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEPSTGC 563
                LD     +VV +NA P   S         TLQL  IQ  + +  + N     + G 
Sbjct: 1025 AGASLDSRLDGLVVAINAAPE--SRTLNEFAGETLQLSAIQQTAGENSLANGVQIAADGT 1072

Query: 564  FMVPSRTTSV 568
              +P+ + +V
Sbjct: 1085 VTLPAWSVAV 1072

[Close]
BLAST of Carg20714 vs. ExPASy Swiss-Prot
Match: P07811 (Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1)

HSP 1 Score: 376.3 bits (965), Expect = 6.1e-103
Identity = 204/477 (42.77%), Postives = 292/477 (61.22%), Query Frame = 0 

Query: 24   DSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKMVQALNQ-IGL 83
            D+ + Q+L   +  +D YNWGY+P  + VP+GSYA+DP G  R+ EFR M+QA+ Q +G+
Sbjct: 555  DNPQVQALNTLVAQTDSYNWGYDPFHYTVPEGSYATDPEGTARIKEFRTMIQAIKQDLGM 614

Query: 84   RVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRN-IDGFIENSTCVNNTASEHFMVERM 143
             V++DVVYNH +  GP D +SVLDKIVP YY R N   G +E++TC +++A EH M  ++
Sbjct: 615  NVIMDVVYNHTNAAGPTDRTSVLDKIVPWYYQRLNETTGSVESATCCSDSAPEHRMFAKL 674

Query: 144  IVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWD 203
            I D +  W  DYK+DGFRFDLMG+   + +L A   ++ L  +        IY +GEGWD
Sbjct: 675  IADSLAVWTTDYKIDGFRFDLMGYHPKAQILSAWERIKALNPD--------IYFFGEGWD 734

Query: 204  FGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPF--GHPLQ--QGFVTGLLLEP 263
              +   + R   ASQ NL GTGIG+F+DR+RDA+ GG PF  G  L+  QG  +G  + P
Sbjct: 735  SNQ---SDRFEIASQINLKGTGIGTFSDRLRDAVRGGGPFDSGDALRQNQGVGSGAGVLP 794

Query: 264  NDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYAS 323
            N+    +D  A        D  ++ MA NL D+VL + +G   +GSE+  Y+G+   YA+
Sbjct: 795  NELTTLSDDQARHL----ADLTRLGMAGNLADFVLIDKDGAVKRGSEI-DYNGAPGGYAA 854

Query: 324  CPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDEL 383
             PTE VNYVS HDN+TL+D++S K  +   ++ R RM  ++ + + L QGI F   G EL
Sbjct: 855  DPTEVVNYVSKHDNQTLWDMISYKAAQEADLDTRVRMQAVSLATVMLGQGIAFDQQGSEL 914

Query: 384  LRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKS 443
            LRSKS  RDSY+SGDWFNR+D++   NN+ VG+P    +  N+ +I  R+ D    P ++
Sbjct: 915  LRSKSFTRDSYDSGDWFNRVDYSLQDNNYNVGMPRSSDDGSNYDII-ARVKDAVATPGET 974

Query: 444  HILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQE 495
             +      +  L  +R SSPLF L     + KRV F NTG     G++VM+I+DG +
Sbjct: 975  ELKQMTAFYQELTALRKSSPLFTLGDGATVMKRVDFRNTGADQQTGLLVMTIDDGMQ 1014

[Close]
BLAST of Carg20714 vs. ExPASy Swiss-Prot
Match: C0SPA0 (Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1)

HSP 1 Score: 195.3 bits (495), Expect = 1.9e-48
Identity = 146/445 (32.81%), Postives = 210/445 (47.19%), Query Frame = 0 

Query: 39  DGYNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCG 98
           D YNWGYNP+ +  P+GSYAS+P+ P  R  E ++M+  L+Q GLRV+LDVV+NH++   
Sbjct: 286 DAYNWGYNPLHFFAPEGSYASNPHDPQTRKTELKQMINTLHQHGLRVILDVVFNHVYK-- 345

Query: 99  PFDPSSVLDKIVPGYYLRRNIDGFIENSTCV-NNTASEHFMVERMIVDDILHWVVDYKVD 158
               +S  +K VPGY+ R +  G   N T V N+ ASE  M  + I D +++W+ +Y VD
Sbjct: 346 --RENSPFEKTVPGYFFRHDECGMPSNGTGVGNDIASERRMARKFIADCVVYWLEEYNVD 405

Query: 159 GFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRG---VN 218
           GFRFDL+G L   T+L  K   +  T+ K G     I L+GEGWD      + +     N
Sbjct: 406 GFRFDLLGILDIDTVLYMK---EKATKAKPG-----ILLFGEGWDLATPLPHEQKAALAN 465

Query: 219 ASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLA 278
           A +      GIG FND  RDA+ G +   H    GF  G                     
Sbjct: 466 APRM----PGIGFFNDMFRDAVKGNT--FHLKATGFALG--------------------- 525

Query: 279 VSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNET 338
            + +  Q  M      + +A   G +     V             P++++NYV +HDN T
Sbjct: 526 -NGESAQAVM------HGIAGSSGWKALAPIVPE-----------PSQSINYVESHDNHT 585

Query: 339 LFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDW 398
            +D +S   P+      R R   LA +II L+QG+PF H G E  R+K    +SY S D 
Sbjct: 586 FWDKMSFALPQENDSRKRSR-QRLAAAIILLAQGVPFIHSGQEFFRTKQGVENSYQSSDS 645

Query: 399 FNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK--------------PSKSHI 458
            N+LD       W      KE   Y   LI  R A P+F+                K H+
Sbjct: 646 INQLD-------WDRRETFKEDVHYIRRLISLRKAHPAFRLRSAADIQRHLECLTLKEHL 665

[Close]
BLAST of Carg20714 vs. ExPASy Swiss-Prot
Match: O33840 (Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / NBRC 100826 / MSB8) OX=243274 GN=pulA PE=1 SV=2)

HSP 1 Score: 191.0 bits (484), Expect = 3.6e-47
Identity = 142/494 (28.74%), Postives = 222/494 (44.94%), Query Frame = 0 

Query: 41  YNWGYNPILWGVPKGSYASDPNGP-CRLIEFRKMVQALNQIGLRVVLDVVYNHLHGCGPF 100
           YNWGY+P L+ VP+G Y++DP  P  R+ E ++MV+AL++ G+ V++D+V+ H +G G  
Sbjct: 416 YNWGYDPYLFMVPEGRYSTDPKNPHTRIREVKEMVKALHKHGIGVIMDMVFPHTYGIGEL 475

Query: 101 DPSSVLDKIVPGYYLRRNIDG-FIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGF 160
              S  D+ VP Y+ R +  G ++  S C N  ASE  M+ + IVD + +WV +Y +DGF
Sbjct: 476 ---SAFDQTVPYYFYRIDKTGAYLNESGCGNVIASERPMMRKFIVDTVTYWVKEYHIDGF 535

Query: 161 RFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFN 220
           RFD MG +   TML+ + AL  +          +I LYGE W        G  +   + +
Sbjct: 536 RFDQMGLIDKKTMLEVERALHKI--------DPTIILYGEPW-----GGWGAPIRFGKSD 595

Query: 221 LFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLAVSKDH 280
           + GT + +FND  RDA + GS F +P  +GFV G          G  K+ +    V    
Sbjct: 596 VAGTHVAAFNDEFRDA-IRGSVF-NPSVKGFVMG----------GYGKETKIKRGVVGS- 655

Query: 281 IQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYASCPTETVNYVSAHDNETLFD-- 340
                          N++GK +K            ++A  P ET+NY + HDN TL+D  
Sbjct: 656 --------------INYDGKLIK------------SFALDPEETINYAACHDNHTLWDKN 715

Query: 341 --VVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDELLRSKSMDRDSYNSGDWF 400
                    +  T  +      LA +I+  SQG+PF H G +  R+K+ + +SYN+    
Sbjct: 716 YLAAKADKKKEWTEEELKNAQKLAGAILLTSQGVPFLHGGQDFCRTKNFNDNSYNAPISI 775

Query: 401 NRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFKPSKSHILAAVENFTNLLQIRY 460
           N  D+                                    K   +        L+++R 
Sbjct: 776 NGFDY----------------------------------ERKLQFIDVFNYHKGLIKLRK 805

Query: 461 SSPLFRLKTANAIQKRVRFHNTGTSLIPG---VIVMSIEDGQEGIPGLTQLDPIYSYIVV 520
             P FRLK A  I+K + F       +PG   ++   ++D   G P        +  IVV
Sbjct: 836 EHPAFRLKNAEEIKKHLEF-------LPGGRRIVAFMLKDHAGGDP--------WKDIVV 805

Query: 521 VVNARPSEISFPCP 526
           + N    + ++  P
Sbjct: 896 IYNGNLEKTTYKLP 805

[Close]
BLAST of Carg20714 vs. ExPASy TrEMBL
Match: A0A6J1G1P3 (pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 SV=1)

HSP 1 Score: 1160.2 bits (3000), Expect = 0.0e+00
Identity = 562/562 (100.00%), Postives = 562/562 (100.00%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM
Sbjct: 405 DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS
Sbjct: 465 VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI
Sbjct: 525 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL
Sbjct: 585 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV
Sbjct: 645 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC
Sbjct: 705 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
           EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP
Sbjct: 885 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 944

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVPSRTTSVFVELRKHE
Sbjct: 945 STGCFMVPSRTTSVFVELRKHE 966

[Close]
BLAST of Carg20714 vs. ExPASy TrEMBL
Match: A0A0A0KTI2 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1)

HSP 1 Score: 1067.0 bits (2758), Expect = 2.8e-308
Identity = 513/562 (91.28%), Postives = 537/562 (95.55%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D ELLE LPPDSAEQQSLIADIQNSDGYNWGYNPI+WGVPKGSYASD NGPCRL+EFRKM
Sbjct: 399 DTELLENLPPDSAEQQSLIADIQNSDGYNWGYNPIMWGVPKGSYASDSNGPCRLVEFRKM 458

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHG GPFDP+SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 459 VQALNQIGLRVVLDVVYNHLHGHGPFDPNSVLDKIVPGYYLRRNTDGFIENSTCVNNTAS 518

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+AL+ LT+EKNGVDGSSI
Sbjct: 519 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALRGLTKEKNGVDGSSI 578

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           Y+YGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGL
Sbjct: 579 YIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGL 638

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGT + AE  LAVSKDHI+VAMAANL+DY+L NFEGKEVKG EV T+D S V
Sbjct: 639 LLEPNDHDHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPV 698

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFD+VSLKTPRNITV+DRCR+NHLATSIIALSQGIPFFHC
Sbjct: 699 AYASCPTETVNYVSAHDNETLFDIVSLKTPRNITVDDRCRINHLATSIIALSQGIPFFHC 758

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 759 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 818

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKT+NAIQKRVRFHN+GTSLIPG+IVMSIEDG 
Sbjct: 819 PSKSHILAAVENFTNLLQIRYSSPLFRLKTSNAIQKRVRFHNSGTSLIPGLIVMSIEDGH 878

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
            GIPGL+QLD  YSYIVVVVNARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEP
Sbjct: 879 NGIPGLSQLDSTYSYIVVVVNARPTEISFPCPALRAKTLQLHPIQLMSTDPVVKNSTYEP 938

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVP RTTSVFVE R HE
Sbjct: 939 STGCFMVPPRTTSVFVEPRMHE 960

[Close]
BLAST of Carg20714 vs. ExPASy TrEMBL
Match: A0A5A7SZ60 (Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 PE=4 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.7e-303
Identity = 508/562 (90.39%), Postives = 532/562 (94.66%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D ELLE LPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKM
Sbjct: 405 DTELLENLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLH  GP D +SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 465 VQALNQIGLRVVLDVVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSI
Sbjct: 525 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           Y+YGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGL
Sbjct: 585 YIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDH HGT + AE  LAVSKDHI+VAMAANL+DY+L NFEGKEVKG EV T+D S V
Sbjct: 645 LLEPNDHHHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFD+VSLKT RNITV+DRCR+NHLATSIIALSQGIPFFHC
Sbjct: 705 AYASCPTETVNYVSAHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHC 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSS LFRLKT++AIQKRVRFHN+GTSLIPG+IVMSIEDG 
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGH 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
           EGIPGL+QLD  YSYIVVV NARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEP
Sbjct: 885 EGIPGLSQLDSTYSYIVVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEP 944

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCF+VP RTTSVFVE R HE
Sbjct: 945 STGCFVVPPRTTSVFVEPRIHE 966

[Close]
BLAST of Carg20714 vs. ExPASy TrEMBL
Match: A0A1S3BGM5 (pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1)

HSP 1 Score: 1050.4 bits (2715), Expect = 2.7e-303
Identity = 508/562 (90.39%), Postives = 532/562 (94.66%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D ELLE LPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRL+EFRKM
Sbjct: 405 DTELLENLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLVEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLH  GP D +SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 465 VQALNQIGLRVVLDVVYNHLHAHGPLDSNSVLDKIVPGYYLRRNPDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLM STMLKAK+ALQ LT+EKNGVDGSSI
Sbjct: 525 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMKSTMLKAKDALQGLTKEKNGVDGSSI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           Y+YGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDA+LGGSPFGHPLQQGFVTGL
Sbjct: 585 YIYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAILGGSPFGHPLQQGFVTGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDH HGT + AE  LAVSKDHI+VAMAANL+DY+L NFEGKEVKG EV T+D S V
Sbjct: 645 LLEPNDHHHGTHEVAESMLAVSKDHIEVAMAANLRDYILTNFEGKEVKGLEVLTHDRSPV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYASCPTETVNYVSAHDNETLFD+VSLKT RNITV+DRCR+NHLATSIIALSQGIPFFHC
Sbjct: 705 AYASCPTETVNYVSAHDNETLFDIVSLKTSRNITVDDRCRINHLATSIIALSQGIPFFHC 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLP KEKN+YNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPLKEKNQYNWPLIKPRLADPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSS LFRLKT++AIQKRVRFHN+GTSLIPG+IVMSIEDG 
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSRLFRLKTSHAIQKRVRFHNSGTSLIPGLIVMSIEDGH 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
           EGIPGL+QLD  YSYIVVV NARP+EISFPCPALRA+TLQLHPIQLMSTDP+VKNS YEP
Sbjct: 885 EGIPGLSQLDSTYSYIVVVFNARPTEISFPCPALRAKTLQLHPIQLMSTDPIVKNSTYEP 944

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCF+VP RTTSVFVE R HE
Sbjct: 945 STGCFVVPPRTTSVFVEPRIHE 966

[Close]
BLAST of Carg20714 vs. ExPASy TrEMBL
Match: A0A6J1HT34 (LOW QUALITY PROTEIN: pullulanase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111466529 PE=4 SV=1)

HSP 1 Score: 1025.8 bits (2651), Expect = 7.0e-296
Identity = 507/562 (90.21%), Postives = 511/562 (90.93%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM
Sbjct: 405 DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALNQIGLRVVLDVVYNHLHGCGP DPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS
Sbjct: 465 VQALNQIGLRVVLDVVYNHLHGCGPLDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILT EKNGVDGSSI
Sbjct: 525 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTLEKNGVDGSSI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL
Sbjct: 585 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LLEPNDHDHGTDKDAEFTLA+SKDHIQVAMAANL+DYVL NFEGKEVKGSE+RTYDGSSV
Sbjct: 645 LLEPNDHDHGTDKDAEFTLAMSKDHIQVAMAANLRDYVLTNFEGKEVKGSELRTYDGSSV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AY                 TLF                            L +GIPFFHC
Sbjct: 705 AYXI---------------TLF----------------------------LFKGIPFFHC 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK
Sbjct: 765 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ
Sbjct: 825 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
           EGIPGLTQLDPIYSYIVVVVNARPSEISFPCP LRARTLQLHPIQLMSTDPMVKNSAYEP
Sbjct: 885 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPVLRARTLQLHPIQLMSTDPMVKNSAYEP 923

Query: 554 STGCFMVPSRTTSVFVELRKHE 576
           STGCFMVPSRTTSVFVELR HE
Sbjct: 945 STGCFMVPSRTTSVFVELRXHE 923

[Close]
BLAST of Carg20714 vs. TAIR 10
Match: AT5G04360.1 (limit dextrinase )

HSP 1 Score: 887.1 bits (2291), Expect = 7.5e-258
Identity = 415/559 (74.24%), Postives = 480/559 (85.87%), Query Frame = 0 

Query: 14  DIELLEKLPPDSAEQQSLIADIQNSDGYNWGYNPILWGVPKGSYASDPNGPCRLIEFRKM 73
           D  LLE L PDS E Q+ I +IQN DGYNWGYNP+LWGVPKGSYASDP GPCR+IEFRKM
Sbjct: 405 DTSLLEGLRPDSTEAQARITEIQNDDGYNWGYNPVLWGVPKGSYASDPTGPCRIIEFRKM 464

Query: 74  VQALNQIGLRVVLDVVYNHLHGCGPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTAS 133
           VQALN  GL VVLDVVYNHLH  GP D  SVLDKIVPGYYLRRN DGFIENSTCVNNTAS
Sbjct: 465 VQALNCTGLNVVLDVVYNHLHASGPHDKESVLDKIVPGYYLRRNSDGFIENSTCVNNTAS 524

Query: 134 EHFMVERMIVDDILHWVVDYKVDGFRFDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSI 193
           EH+MV+R+I DD+L+WVV+YKVDGFRFDLMGH+M +T++ AK+A+  L +E +GVDGS I
Sbjct: 525 EHYMVDRLIRDDLLNWVVNYKVDGFRFDLMGHIMKATIVNAKSAIGSLRKETDGVDGSRI 584

Query: 194 YLYGEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGL 253
           YLYGEGW+FGEVA+NGRG+NASQFNL GTGIGSFNDR+RDA LGGSPFGHPLQQGF+TGL
Sbjct: 585 YLYGEGWNFGEVAENGRGINASQFNLGGTGIGSFNDRIRDATLGGSPFGHPLQQGFITGL 644

Query: 254 LLEPNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSV 313
           LL+PN HDHG++   E  L+ +K+HIQ  MAANLKDY+L N EGKEVKGSEV  +D + V
Sbjct: 645 LLQPNAHDHGSEATQELMLSTAKNHIQTGMAANLKDYMLTNHEGKEVKGSEVLMHDATPV 704

Query: 314 AYASCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHC 373
           AYAS PTET+NYVSAHDNETLFD++SLKTP  I+V++RCR+NHLA+S+IALSQGIPFFH 
Sbjct: 705 AYASLPTETINYVSAHDNETLFDIISLKTPMEISVDERCRINHLASSMIALSQGIPFFHA 764

Query: 374 GDELLRSKSMDRDSYNSGDWFNRLDFTYMTNNWGVGLPPKEKNEYNWPLIKPRLADPSFK 433
           GDE+LRSKS+DRDSYNSGDWFNRLDF+Y +NNWGVGLPPK KNE+NWPLIKPRL DPSFK
Sbjct: 765 GDEILRSKSLDRDSYNSGDWFNRLDFSYSSNNWGVGLPPKGKNEHNWPLIKPRLQDPSFK 824

Query: 434 PSKSHILAAVENFTNLLQIRYSSPLFRLKTANAIQKRVRFHNTGTSLIPGVIVMSIEDGQ 493
           P  SHI+A + NF +LL+IRYSSPLFRL TA AIQ+RVRFHNTG S IPG IVMSIEDG 
Sbjct: 825 PKSSHIVATLHNFLDLLRIRYSSPLFRLDTARAIQERVRFHNTGPSSIPGAIVMSIEDGH 884

Query: 494 EGIPGLTQLDPIYSYIVVVVNARPSEISFPCPALRARTLQLHPIQLMSTDPMVKNSAYEP 553
            GIP ++Q+DPIYS IVV+ NARPSE S+P PAL+ R L+LHP+Q+MS D +VK S Y+ 
Sbjct: 885 RGIPSVSQIDPIYSLIVVIFNARPSEFSYPSPALKDRKLELHPVQVMSADEIVKKSVYDS 944

Query: 554 STGCFMVPSRTTSVFVELR 573
            +G F VP+RTT+VFVE R
Sbjct: 945 FSGGFTVPARTTTVFVESR 963

[Close]
BLAST of Carg20714 vs. TAIR 10
Match: AT4G09020.1 (isoamylase 3 )

HSP 1 Score: 95.5 bits (236), Expect = 1.5e-19
Identity = 98/391 (25.06%), Postives = 142/391 (36.32%), Query Frame = 0 

Query: 43  WGYNPILWGVPKGSYASDPNGPCRLI-EFRKMVQALNQIGLRVVLDVVYNHLHGCGPFDP 102
           WGY+ + +  P   YAS    P +   EF++MV+AL+  G+ V+LDVVYNH +      P
Sbjct: 307 WGYSTVNFFAPMSRYASGEGDPIKASKEFKEMVKALHSAGIEVILDVVYNHTNEADDKYP 366

Query: 103 SSVLDKIVPG--YYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVDGFR 162
            +   + +    YY+    +  +  S C N     H +V  +I+D + HWV +Y VDGFR
Sbjct: 367 YTTSFRGIDNKVYYMLDPNNQLLNFSGCGNTLNCNHPVVMELILDSLRHWVTEYHVDGFR 426

Query: 163 FDLMGHLMTSTMLKAKNALQILTQEKNGVDGSSIYLYGEGWDFGEVAKNGRGVNASQFNL 222
           FDL   L  +T     +A  ++         S   +  E WD G +   G+  N   ++ 
Sbjct: 427 FDLASVLCRATDGSPLSAPPLIRAIAKDSVLSRCKIIAEPWDCGGLYLVGKFPN---WDR 486

Query: 223 FGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLEPNDHDHGTDKDAEFTLAVSKDHI 282
           +    G + D VR  + G S                                        
Sbjct: 487 WAEWNGMYRDDVRRFIKGDS---------------------------------------- 546

Query: 283 QVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAY---ASCPTETVNYVSAHDNETLFD 342
                               +KGS      GSS  Y      P   VN+V AHD  TL D
Sbjct: 547 -------------------GMKGSFATRVSGSSDLYQVNQRKPYHGVNFVIAHDGFTLRD 606

Query: 343 VVSLKTPRNIT----VNDRCRMNH-----------------LATS-------IIALSQGI 400
           +VS     N       ND C  NH                 L T         + +SQG 
Sbjct: 607 LVSYNFKHNEANGEGGNDGCNDNHSWNCGFEGETGDAHIKSLRTRQMKNFHLALMISQGT 635

[Close]
BLAST of Carg20714 vs. TAIR 10
Match: AT2G39930.1 (isoamylase 1 )

HSP 1 Score: 81.6 bits (200), Expect = 2.2e-15
Identity = 98/379 (25.86%), Postives = 150/379 (39.58%), Query Frame = 0 

Query: 43  WGYNPILWGVPKGSY--ASDPNGPCRLI-EFRKMVQALNQIGLRVVLDVVYNHL---HGC 102
           WGY+ I +  P   Y  AS  N   R I EF+ +V+  ++ G+ V++DVV NH    +  
Sbjct: 288 WGYSTIGFFSPMIRYASASSNNFAGRAINEFKILVKEAHKRGIEVIMDVVLNHTAEGNEK 347

Query: 103 GPFDPSSVLDKIVPGYYLRRNIDGFIENSTCVNNTASEHFMVERMIVDDILHWVVDYKVD 162
           GP      +D  V  YY+      F   S C N     H +V + I+D + +WV +  VD
Sbjct: 348 GPIFSFRGVDNSV--YYMLAPKGEFYNYSGCGNTFNCNHPVVRQFILDCLRYWVTEMHVD 407

Query: 163 GFRFDLMGHLM--TSTMLKAKNAL------------------QILTQEKNGVDGSSIYLY 222
           GFRFDL G +M  +S++  A N                     ++    N      + L 
Sbjct: 408 GFRFDL-GSIMSRSSSLWDAANVYGADVEGDLLTTGTPISCPPVIDMISNDPILRGVKLI 467

Query: 223 GEGWDFGEVAKNGRGVNASQFNLFGTGIGSFNDRVRDAMLGGSPFGHPLQQGFVTGLLLE 282
            E WD G + + G       + ++    G F D VR  + G   F       F   L   
Sbjct: 468 AEAWDAGGLYQVGM---FPHWGIWSEWNGKFRDVVRQFIKGTDGF----SGAFAECLCGS 527

Query: 283 PNDHDHGTDKDAEFTLAVSKDHIQVAMAANLKDYVLANFEGKEVKGSEVRTYDGSSVAYA 342
           PN +  G           + D   +A      D V  N +     G E    DG      
Sbjct: 528 PNLYQGGRKPWHSINFICAHDGFTLA------DLVTYNNKNNLANGEE--NNDG------ 587

Query: 343 SCPTETVNYVSAHDNETLFDVVSLKTPRNITVNDRCRMNHLATSIIALSQGIPFFHCGDE 396
               E  NY      E  F  +S+K  R      + +M +   S++ +SQG+P  + GDE
Sbjct: 588 ----ENHNYSWNCGEEGDFASISVKRLR------KRQMRNFFVSLM-VSQGVPMIYMGDE 631

The following BLAST results are available for this feature:
BLAST of Carg20714 vs. NCBI nr
Match NameE-valueIdentityDescription
KAG7012918.10.0e+00100.00Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperm... [more]
XP_022945721.10.0e+00100.00pullulanase 1, chloroplastic [Cucurbita moschata][more]
KAG6573853.10.0e+0099.82Pullulanase 1, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_031741579.15.7e-30891.28pullulanase 1, chloroplastic isoform X2 [Cucumis sativus][more]
KAE8648841.15.7e-30891.28hypothetical protein Csa_008972 [Cucumis sativus][more]
BLAST of Carg20714 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q8GTR41.1e-25674.24Pullulanase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=PU1 PE=1 SV=2[more]
P072061.0e-10539.82Pullulanase OS=Klebsiella pneumoniae OX=573 GN=pulA PE=1 SV=2[more]
P078116.1e-10342.77Pullulanase OS=Klebsiella aerogenes OX=548 GN=pulA PE=1 SV=1[more]
C0SPA01.9e-4832.81Pullulanase OS=Bacillus subtilis (strain 168) OX=224308 GN=amyX PE=1 SV=1[more]
O338403.6e-4728.74Pullulanase OS=Thermotoga maritima (strain ATCC 43589 / DSM 3109 / JCM 10099 / N... [more]
BLAST of Carg20714 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1G1P30.0e+00100.00pullulanase 1, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111449862 PE=4 ... [more]
A0A0A0KTI22.8e-30891.28Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G622510 PE=4 SV=1[more]
A0A5A7SZ602.7e-30390.39Pullulanase 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold65G007110 ... [more]
A0A1S3BGM52.7e-30390.39pullulanase 1, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489432 PE=4 SV=1[more]
A0A6J1HT347.0e-29690.21LOW QUALITY PROTEIN: pullulanase 1, chloroplastic OS=Cucurbita maxima OX=3661 GN... [more]
BLAST of Carg20714 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G04360.17.5e-25874.24limit dextrinase [more]
AT4G09020.11.5e-1925.06isoamylase 3 [more]
AT2G39930.12.2e-1525.86isoamylase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR013780Glycosyl hydrolase, all-betaGENE3D2.60.40.1180coord: 496..572
e-value: 1.2E-26
score: 94.5
IPR024561Alpha-1,6-glucosidases, pullulanase-type, C-terminalPFAMPF11852DUF3372coord: 402..570
e-value: 6.3E-63
score: 211.2
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 7..495
e-value: 8.0E-209
score: 696.7
NoneNo IPR availableCDDcd11341AmyAc_Pullulanase_LD-likecoord: 34..405
e-value: 8.40816E-150
score: 435.014
NoneNo IPR availableSUPERFAMILY51011Glycosyl hydrolase domaincoord: 463..573
IPR011839Alpha-1,6-glucosidases, pullulanase-typeTIGRFAMTIGR02103TIGR02103coord: 17..572
e-value: 6.1E-251
score: 833.2
IPR045041Pullulanase PULA-likePANTHERPTHR43631PULLULANASE 1, CHLOROPLASTICcoord: 13..571
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 16..460

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20714-RACarg20714-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0051060 pullulanase activity