Carg20543 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20543
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	DUF4057 domain-containing protein
Location	Carg_Chr12: 808341 .. 810110 (-)
RNA-Seq Expression	Carg20543
Synteny	Carg20543

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TCTTCTTCGCTTCCATTTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTGCAATTCTTCCCAATTTGTTTCTTCTTCTTCTTCTTCTTCTTCAATGGACAGAGCTACTCCTGTTCGGAAGCCTCACACCTCTACTGCAGATCTGCTCACTTGGCCGGAAGTTCCGCCGGCTGATTCCTCTGCGCTTTCTTCTTCTGCTTCTCGCTCTGCTCCGAGGTCTCATCAGGTACTTTCTACATTTTTATCTTTTTCCTTTTCTTCTTTCCTTTCGTCTTCTTCTGCTTATTCTGTTTGTTTTTGTTGTTTTTGTAGCCCTCCCCTGGGATTAGTAAGGTCGTCTTTGGAGGTCAGGTCACGGATGAGGAGGTTGAGAGCTTGAACAAAAGGTGAGATCTAAATCGGTTTTGTTCTTGCTTATTTCATTCCTGTTATTTTGGATAATTTTTGTTTCGGTTTCTTTCTTGTGCCGAATTGTGAGTACTGTGAATTGTTAAGGTGGAAACTCGAATGAGCTAATCTGTTTCGTTTCGGTGTTTCCTTGATGTTGAGAAGACTGTTTTGTTCTCTCTCCCTATTTCTCATTCGCTGCTATTTTGTCGCTCTTTTTTAGTTGTGTAGCGCTTGTTGAATTTGTATCATTTCTATAAACCTCCGATCTGCTTGCTTAGTTTGTAAAAATTGGCCAAGTTATTCAAATTTCAAATCTCTATCTCTTCCTCCTTGCGTGGTAATATTCGTTTTGCTCAAATGACTGCTGATTGGATCTCAAATCTGTATTCCTTTCTGGTTTTCAAGGCATTTTCACAAATCTTTGATCAAATTTGTTCGTCCTTCCCGCATCAATTGATTTTAACGTCATTTGAATACAAATTTTCAAGTTGCTCTACAATCTTGAATTTGCTGGAGTCGTTTAGCTTGAGTTTCATATGAGGCGTCTCATTTGTTGTTCAATTTGCAAACACCAGGAAACCCTGTTCTGGATATAAAATGAAGGAGATGACTGGCAGTGGCATTTTTGTTGCAAACGAAGAAGATGATGAACTAGAATCTGGAAGCGCCAACCCTTCACAGAATAAAACAGGAATACGTATGTACCAGGTATCCATCGAAGAAGATCATCATTTATTGCAGTTTATCTGTGACTGTGGGAAGAATTTGTGTTTGGTTAAGGCTTTTCTAACTTCTATTCTTGACGGCTCGATCAACTGCAGCAAGCCTTGGCTGGGATTAGTCATATTTCATTCGGTGAAGAAGGAAGTGTTTCTCCTAAAAAACCAACTACTCTACCCGAGGTTGCGAAGCAGCGTGAGCTGAGCGGGAACCTAGAAAGCGATGCCGATGCAATGTTGAAGAAGCAGCTTTCTGATGCTAAATGCAAGGAGCTTAGTGGACATGATATATTCGCGCCTCCTCCTGAGATCTTACCCCGACCAACAACTGCACGCACTTTGGATTTAAAAGGAAGCATTGAGATTGGGGAGCCTGATAATGTAAGTAGTACTCTTCTGATCAAGTAATTTTCAGGATGGAATTCTAGTCATTCTAATTTCTAATACATCCATTGAGAAGGCACACCCCACAAACGACAATTCTCATTTTGCTTAGAAATTACATAATTTAGTGTTAGGTTCTCAGCCATAGCTCGCTTGTTCGTTCGTTCGTTCGTCAGGCTGCATGGATAGATGGGTGTTGCATCAACCAATTTATCTTACTCGTTTGTTTGCATCTTAGTATCCTGATGGGGTTAGAATCTTAATGATGAAATCCTAGG

mRNA sequence

TCTTCTTCGCTTCCATTTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTGCAATTCTTCCCAATTTGTTTCTTCTTCTTCTTCTTCTTCTTCAATGGACAGAGCTACTCCTGTTCGGAAGCCTCACACCTCTACTGCAGATCTGCTCACTTGGCCGGAAGTTCCGCCGGCTGATTCCTCTGCGCTTTCTTCTTCTGCTTCTCGCTCTGCTCCGAGGTCTCATCAGCCCTCCCCTGGGATTAGTAAGGTCGTCTTTGGAGGTCAGGTCACGGATGAGGAGGTTGAGAGCTTGAACAAAAGGAAACCCTGTTCTGGATATAAAATGAAGGAGATGACTGGCAGTGGCATTTTTGTTGCAAACGAAGAAGATGATGAACTAGAATCTGGAAGCGCCAACCCTTCACAGAATAAAACAGGAATACGTATGTACCAGCAAGCCTTGGCTGGGATTAGTCATATTTCATTCGGTGAAGAAGGAAGTGTTTCTCCTAAAAAACCAACTACTCTACCCGAGGTTGCGAAGCAGCGTGAGCTGAGCGGGAACCTAGAAAGCGATGCCGATGCAATGTTGAAGAAGCAGCTTTCTGATGCTAAATGCAAGGAGCTTAGTGGACATGATATATTCGCGCCTCCTCCTGAGATCTTACCCCGACCAACAACTGCACGCACTTTGGATTTAAAAGGAAGCATTGAGATTGGGGAGCCTGATAATGTAAGTAGTACTCTTCTGATCAAGTAATTTTCAGGATGGAATTCTAGTCATTCTAATTTCTAATACATCCATTGAGAAGGCACACCCCACAAACGACAATTCTCATTTTGCTTAGAAATTACATAATTTAGTGTTAGGTTCTCAGCCATAGCTCGCTTGTTCGTTCGTTCGTTCGTCAGGCTGCATGGATAGATGGGTGTTGCATCAACCAATTTATCTTACTCGTTTGTTTGCATCTTAGTATCCTGATGGGGTTAGAATCTTAATGATGAAATCCTAGG

Coding sequence (CDS)

TCTTCTTCGCTTCCATTTCTCTCTCTCTCTCTCTCTCTCTCTCTCCTCTGCAATTCTTCCCAATTTGTTTCTTCTTCTTCTTCTTCTTCTTCAATGGACAGAGCTACTCCTGTTCGGAAGCCTCACACCTCTACTGCAGATCTGCTCACTTGGCCGGAAGTTCCGCCGGCTGATTCCTCTGCGCTTTCTTCTTCTGCTTCTCGCTCTGCTCCGAGGTCTCATCAGCCCTCCCCTGGGATTAGTAAGGTCGTCTTTGGAGGTCAGGTCACGGATGAGGAGGTTGAGAGCTTGAACAAAAGGAAACCCTGTTCTGGATATAAAATGAAGGAGATGACTGGCAGTGGCATTTTTGTTGCAAACGAAGAAGATGATGAACTAGAATCTGGAAGCGCCAACCCTTCACAGAATAAAACAGGAATACGTATGTACCAGCAAGCCTTGGCTGGGATTAGTCATATTTCATTCGGTGAAGAAGGAAGTGTTTCTCCTAAAAAACCAACTACTCTACCCGAGGTTGCGAAGCAGCGTGAGCTGAGCGGGAACCTAGAAAGCGATGCCGATGCAATGTTGAAGAAGCAGCTTTCTGATGCTAAATGCAAGGAGCTTAGTGGACATGATATATTCGCGCCTCCTCCTGAGATCTTACCCCGACCAACAACTGCACGCACTTTGGATTTAAAAGGAAGCATTGAGATTGGGGAGCCTGATAATGTAAGTAGTACTCTTCTGATCAAGTAA

Protein sequence

SSSLPFLSLSLSLSLLCNSSQFVSSSSSSSSMDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTDEEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGISHISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPPPEILPRPTTARTLDLKGSIEIGEPDNVSSTLLIK

Homology

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BLAST of Carg20543 vs. NCBI nr
Match: KAG7020211.1 (DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 463.8 bits (1192), Expect = 9.4e-127
Identity = 245/245 (100.00%), Postives = 245/245 (100.00%), Query Frame = 0


Query: 1   SSSLPFLSLSLSLSLLCNSSQFVSSSSSSSSMDRATPVRKPHTSTADLLTWPEVPPADSS 60
           SSSLPFLSLSLSLSLLCNSSQFVSSSSSSSSMDRATPVRKPHTSTADLLTWPEVPPADSS
Sbjct: 1   SSSLPFLSLSLSLSLLCNSSQFVSSSSSSSSMDRATPVRKPHTSTADLLTWPEVPPADSS 60

Query: 61  ALSSSASRSAPRSHQPSPGISKVVFGGQVTDEEVESLNKRKPCSGYKMKEMTGSGIFVAN 120
           ALSSSASRSAPRSHQPSPGISKVVFGGQVTDEEVESLNKRKPCSGYKMKEMTGSGIFVAN
Sbjct: 61  ALSSSASRSAPRSHQPSPGISKVVFGGQVTDEEVESLNKRKPCSGYKMKEMTGSGIFVAN 120

Query: 121 EEDDELESGSANPSQNKTGIRMYQQALAGISHISFGEEGSVSPKKPTTLPEVAKQRELSG 180
           EEDDELESGSANPSQNKTGIRMYQQALAGISHISFGEEGSVSPKKPTTLPEVAKQRELSG
Sbjct: 121 EEDDELESGSANPSQNKTGIRMYQQALAGISHISFGEEGSVSPKKPTTLPEVAKQRELSG 180

Query: 181 NLESDADAMLKKQLSDAKCKELSGHDIFAPPPEILPRPTTARTLDLKGSIEIGEPDNVSS 240
           NLESDADAMLKKQLSDAKCKELSGHDIFAPPPEILPRPTTARTLDLKGSIEIGEPDNVSS
Sbjct: 181 NLESDADAMLKKQLSDAKCKELSGHDIFAPPPEILPRPTTARTLDLKGSIEIGEPDNVSS 240

Query: 241 TLLIK 246
           TLLIK
Sbjct: 241 TLLIK 245

[Close]

BLAST of Carg20543 vs. NCBI nr
Match: XP_022951840.1 (uncharacterized protein LOC111454570 [Cucurbita moschata] >XP_023002695.1 uncharacterized protein LOC111496478 [Cucurbita maxima] >XP_023537292.1 uncharacterized protein LOC111798406 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 407.9 bits (1047), Expect = 6.1e-110
Identity = 206/208 (99.04%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD
Sbjct: 1   MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDNVS 240
           PEILPRPTTARTLDLKGSIEIGEPDN++
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDNIN 208

[Close]

BLAST of Carg20543 vs. NCBI nr
Match: KAG6585290.1 (hypothetical protein SDJN03_18023, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 406.4 bits (1043), Expect = 1.8e-109
Identity = 205/208 (98.56%), Postives = 207/208 (99.52%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDRATPVRKPHTSTADLLTWPEVPPADSS LSSSASRSAPRSHQPSPGISKVVFGGQVTD
Sbjct: 1   MDRATPVRKPHTSTADLLTWPEVPPADSSVLSSSASRSAPRSHQPSPGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDNVS 240
           PEILPRPTTARTLDLKGSIEIGEPDN++
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDNIN 208

[Close]

BLAST of Carg20543 vs. NCBI nr
Match: XP_038884071.1 (uncharacterized protein LOC120075008 [Benincasa hispida])

HSP 1 Score: 384.4 bits (986), Expect = 7.3e-103
Identity = 194/206 (94.17%), Postives = 198/206 (96.12%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDR TPVRKPHTSTADLLTWPE+PPADS AL SSASRSAPRSHQPS GISKVVFGGQVTD
Sbjct: 1   MDRTTPVRKPHTSTADLLTWPELPPADSPALPSSASRSAPRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFVANE D+ELESGSANPSQNKTGIRMYQQ L GIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVANEGDEELESGSANPSQNKTGIRMYQQTLTGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPT++PEVAKQRELSGNLESDADA LKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTSVPEVAKQRELSGNLESDADAKLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDN 238
           PEILPRPTTARTLDLKGSIEIGEPDN
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDN 206

[Close]

BLAST of Carg20543 vs. NCBI nr
Match: XP_004142997.1 (uncharacterized protein LOC101215119 [Cucumis sativus] >KGN62250.1 hypothetical protein Csa_018791 [Cucumis sativus])

HSP 1 Score: 384.0 bits (985), Expect = 9.5e-103
Identity = 194/206 (94.17%), Postives = 198/206 (96.12%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDR TPVRKPHTSTADLLTWPE+PPADS AL SSASRSAPRSHQPS GISKVVFGGQVTD
Sbjct: 1   MDRTTPVRKPHTSTADLLTWPELPPADSPALPSSASRSAPRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFV NE D+ELESGSANPSQNKTGIRMYQQ LAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVGNEGDEELESGSANPSQNKTGIRMYQQTLAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTT+PEVAKQRELSGNLESDADA LKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTVPEVAKQRELSGNLESDADAKLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDN 238
           PEILPRPTTARTLDLKGSIEIGEPD+
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDS 206

[Close]

BLAST of Carg20543 vs. ExPASy Swiss-Prot
Match: Q9SIE0 (DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 SV=2)

HSP 1 Score: 52.4 bits (124), Expect = 8.6e-06
Identity = 29/58 (50.00%), Postives = 39/58 (67.24%), Query Frame = 0


Query: 67  SRSAPRSHQPSPGISKVVFGGQVTDEEVESLNKRKPCSGYKMKEMTGSGIFVANEEDD 125
           S +A RS+QPS   S  +  GQ+T+EE ESL  +K CSG+K+KE+T S  F  N +DD
Sbjct: 7   STAANRSNQPS---SDGISDGQITNEEAESLINKKNCSGHKLKEVTDSDTFSDNGKDD 61

[Close]

BLAST of Carg20543 vs. ExPASy TrEMBL
Match: A0A6J1KPP3 (uncharacterized protein LOC111496478 OS=Cucurbita maxima OX=3661 GN=LOC111496478 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.0e-110
Identity = 206/208 (99.04%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD
Sbjct: 1   MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDNVS 240
           PEILPRPTTARTLDLKGSIEIGEPDN++
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDNIN 208

[Close]

BLAST of Carg20543 vs. ExPASy TrEMBL
Match: A0A6J1GK09 (uncharacterized protein LOC111454570 OS=Cucurbita moschata OX=3662 GN=LOC111454570 PE=4 SV=1)

HSP 1 Score: 407.9 bits (1047), Expect = 3.0e-110
Identity = 206/208 (99.04%), Postives = 208/208 (100.00%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD
Sbjct: 1   MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDNVS 240
           PEILPRPTTARTLDLKGSIEIGEPDN++
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDNIN 208

[Close]

BLAST of Carg20543 vs. ExPASy TrEMBL
Match: A0A0A0LK08 (DUF4057 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G345910 PE=4 SV=1)

HSP 1 Score: 384.0 bits (985), Expect = 4.6e-103
Identity = 194/206 (94.17%), Postives = 198/206 (96.12%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDR TPVRKPHTSTADLLTWPE+PPADS AL SSASRSAPRSHQPS GISKVVFGGQVTD
Sbjct: 1   MDRTTPVRKPHTSTADLLTWPELPPADSPALPSSASRSAPRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFV NE D+ELESGSANPSQNKTGIRMYQQ LAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVGNEGDEELESGSANPSQNKTGIRMYQQTLAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTT+PEVAKQRELSGNLESDADA LKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTVPEVAKQRELSGNLESDADAKLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDN 238
           PEILPRPTTARTLDLKGSIEIGEPD+
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDS 206

[Close]

BLAST of Carg20543 vs. ExPASy TrEMBL
Match: A0A5A7V9J6 (DUF4057 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G001450 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.7e-102
Identity = 194/208 (93.27%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDR TPVRKPHTSTADLLTWPE+PPADS AL SSASRSAPRSHQPS GISKVVFGGQVTD
Sbjct: 1   MDRTTPVRKPHTSTADLLTWPELPPADSPALPSSASRSAPRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFV NE D+ELESGSANP QNKTGIRMYQQ LAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVGNEGDEELESGSANPLQNKTGIRMYQQTLAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTT+PEVAKQRELSGNLESDADA LKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTVPEVAKQRELSGNLESDADAKLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDNVS 240
           PEILPRPTTARTLDLKGSIEIGEPD+ S
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDSRS 208

[Close]

BLAST of Carg20543 vs. ExPASy TrEMBL
Match: A0A1S3BD79 (uncharacterized protein LOC103488402 OS=Cucumis melo OX=3656 GN=LOC103488402 PE=4 SV=1)

HSP 1 Score: 382.1 bits (980), Expect = 1.7e-102
Identity = 194/208 (93.27%), Postives = 198/208 (95.19%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           MDR TPVRKPHTSTADLLTWPE+PPADS AL SSASRSAPRSHQPS GISKVVFGGQVTD
Sbjct: 1   MDRTTPVRKPHTSTADLLTWPELPPADSPALPSSASRSAPRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCSGYKMKEMTGSGIFV NE D+ELESGSANP QNKTGIRMYQQ LAGIS
Sbjct: 61  EEVESLNKRKPCSGYKMKEMTGSGIFVGNEGDEELESGSANPLQNKTGIRMYQQTLAGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEEGSVSPKKPTT+PEVAKQRELSGNLESDADA LKKQLSDAKCKELSGHDIFAPP
Sbjct: 121 HISFGEEGSVSPKKPTTVPEVAKQRELSGNLESDADAKLKKQLSDAKCKELSGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPDNVS 240
           PEILPRPTTARTLDLKGSIEIGEPD+ S
Sbjct: 181 PEILPRPTTARTLDLKGSIEIGEPDSRS 208

[Close]

BLAST of Carg20543 vs. TAIR 10
Match: AT1G35780.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G78150.2); Has 145 Blast hits to 144 proteins in 16 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 145; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 254.2 bits (648), Expect = 1.1e-67
Identity = 139/205 (67.80%), Postives = 154/205 (75.12%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           M++ TPVRKPH STADLLTWPE  P +S A  S  SRSA RSHQPS GISKVVFGGQVTD
Sbjct: 1   MEKNTPVRKPHMSTADLLTWPENQPFESPAAVS--SRSAARSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLNKRKPCS YKMKE+TGSGIF   EE+D+ E  SAN + N       Q   A +S
Sbjct: 61  EEVESLNKRKPCSNYKMKEITGSGIFSVYEENDDSELASANSATNGKSRTFQQPPAAIMS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
           HISFGEE  V+PKKP T+PEVAKQRELSG LE  +DA L KQ SDAKCKELSGH+IFAPP
Sbjct: 121 HISFGEEEIVTPKKPATVPEVAKQRELSGTLEYQSDAKLNKQFSDAKCKELSGHNIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEPD 237
           PEI  RP T R L  K + ++GE D
Sbjct: 181 PEIKLRP-TVRALAYKDNFDLGESD 202

[Close]

BLAST of Carg20543 vs. TAIR 10
Match: AT1G78150.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G35780.1); Has 152 Blast hits to 146 proteins in 18 species: Archae - 0; Bacteria - 0; Metazoa - 1; Fungi - 2; Plants - 149; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 251.5 bits (641), Expect = 6.8e-67
Identity = 136/202 (67.33%), Postives = 157/202 (77.72%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           M+R+TPVRKPHTSTADLLTW EVPP DS    SSASRSA RSHQPS GISKVVFGGQVTD
Sbjct: 1   MERSTPVRKPHTSTADLLTWSEVPPPDS---PSSASRSAVRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLN+RKPCS +KMKE+TGSGIF  NE+DD  E            + +YQQA+ GIS
Sbjct: 61  EEVESLNRRKPCSEHKMKEITGSGIFSRNEKDDASEP-----------LPVYQQAVNGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
            ISFGEE ++SPKKP T+PEVAKQRELSG +E+++   L+KQLSDAK KE+SG +IFAPP
Sbjct: 121 QISFGEEENLSPKKPATVPEVAKQRELSGTMENESANKLQKQLSDAKYKEISGQNIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIG 234
           PEI PR  T R L LK +  +G
Sbjct: 181 PEIKPRSGTNRALALKDNFNLG 188

[Close]

BLAST of Carg20543 vs. TAIR 10
Match: AT1G78150.2 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G35780.1); Has 35333 Blast hits to 34131 proteins in 2444 species: Archae - 798; Bacteria - 22429; Metazoa - 974; Fungi - 991; Plants - 531; Viruses - 0; Other Eukaryotes - 9610 (source: NCBI BLink). )

HSP 1 Score: 251.5 bits (641), Expect = 6.8e-67
Identity = 136/202 (67.33%), Postives = 157/202 (77.72%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           M+R+TPVRKPHTSTADLLTW EVPP DS    SSASRSA RSHQPS GISKVVFGGQVTD
Sbjct: 1   MERSTPVRKPHTSTADLLTWSEVPPPDS---PSSASRSAVRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EEVESLN+RKPCS +KMKE+TGSGIF  NE+DD  E            + +YQQA+ GIS
Sbjct: 61  EEVESLNRRKPCSEHKMKEITGSGIFSRNEKDDASEP-----------LPVYQQAVNGIS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
            ISFGEE ++SPKKP T+PEVAKQRELSG +E+++   L+KQLSDAK KE+SG +IFAPP
Sbjct: 121 QISFGEEENLSPKKPATVPEVAKQRELSGTMENESANKLQKQLSDAKYKEISGQNIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIG 234
           PEI PR  T R L LK +  +G
Sbjct: 181 PEIKPRSGTNRALALKDNFNLG 188

[Close]

BLAST of Carg20543 vs. TAIR 10
Match: AT1G78150.3 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G35780.1). )

HSP 1 Score: 236.1 bits (601), Expect = 3.0e-62
Identity = 136/231 (58.87%), Postives = 157/231 (67.97%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           M+R+TPVRKPHTSTADLLTW EVPP DS    SSASRSA RSHQPS GISKVVFGGQVTD
Sbjct: 1   MERSTPVRKPHTSTADLLTWSEVPPPDS---PSSASRSAVRSHQPSDGISKVVFGGQVTD 60

Query: 92  EEVESLNK-----------------------------RKPCSGYKMKEMTGSGIFVANEE 151
           EEVESLN+                             RKPCS +KMKE+TGSGIF  NE+
Sbjct: 61  EEVESLNRRILDDAFDSFMRLVIYTNVKTCENVYDVIRKPCSEHKMKEITGSGIFSRNEK 120

Query: 152 DDELESGSANPSQNKTGIRMYQQALAGISHISFGEEGSVSPKKPTTLPEVAKQRELSGNL 211
           DD  E            + +YQQA+ GIS ISFGEE ++SPKKP T+PEVAKQRELSG +
Sbjct: 121 DDASEP-----------LPVYQQAVNGISQISFGEEENLSPKKPATVPEVAKQRELSGTM 180

Query: 212 ESDADAMLKKQLSDAKCKELSGHDIFAPPPEILPRPTTARTLDLKGSIEIG 234
           E+++   L+KQLSDAK KE+SG +IFAPPPEI PR  T R L LK +  +G
Sbjct: 181 ENESANKLQKQLSDAKYKEISGQNIFAPPPEIKPRSGTNRALALKDNFNLG 217

[Close]

BLAST of Carg20543 vs. TAIR 10
Match: AT4G39860.1 (unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT2G22270.1); Has 152 Blast hits to 146 proteins in 19 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 2; Plants - 146; Viruses - 0; Other Eukaryotes - 4 (source: NCBI BLink). )

HSP 1 Score: 224.9 bits (572), Expect = 6.8e-59
Identity = 120/204 (58.82%), Postives = 147/204 (72.06%), Query Frame = 0


Query: 32  MDRATPVRKPHTSTADLLTWPEVPPADSSALSSSASRSAPRSHQPSPGISKVVFGGQVTD 91
           M+R TPVR PHTSTADLL+W E PP    +  S+A     RSHQPS GISK++ GGQ+TD
Sbjct: 1   MERNTPVRNPHTSTADLLSWSETPPPPHHSTPSAA-----RSHQPSDGISKILGGGQITD 60

Query: 92  EEVESLNKRKPCSGYKMKEMTGSGIFVANEEDDELESGSANPSQNKTGIRMYQQALAGIS 151
           EE +SLNK K CSGYK+KEMTGSGIF    +  ++ S S   +  KTG+R YQQ L G+S
Sbjct: 61  EEAQSLNKLKNCSGYKLKEMTGSGIFT---DKGKVGSESDATTDPKTGLRYYQQTLNGMS 120

Query: 152 HISFGEEGSVSPKKPTTLPEVAKQRELSGNLESDADAMLKKQLSDAKCKELSGHDIFAPP 211
            ISF  +G+VSPKKPTTL EVAKQRELSGNL ++AD    KQ+S AK +E+SGHDIFAPP
Sbjct: 121 QISFSADGNVSPKKPTTLTEVAKQRELSGNLLTEADLKSNKQISSAKIEEISGHDIFAPP 180

Query: 212 PEILPRPTTARTLDLKGSIEIGEP 236
            EI PR   A   + +G+ ++GEP
Sbjct: 181 SEIQPRSLVAAQQEARGNRDMGEP 196

The following BLAST results are available for this feature:

BLAST of Carg20543 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020211.1	9.4e-127	100.00	DNA oxidative demethylase ALKBH2, partial [Cucurbita argyrosperma subsp. argyros...	[more]
XP_022951840.1	6.1e-110	99.04	uncharacterized protein LOC111454570 [Cucurbita moschata] >XP_023002695.1 unchar...	[more]
KAG6585290.1	1.8e-109	98.56	hypothetical protein SDJN03_18023, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_038884071.1	7.3e-103	94.17	uncharacterized protein LOC120075008 [Benincasa hispida]	[more]
XP_004142997.1	9.5e-103	94.17	uncharacterized protein LOC101215119 [Cucumis sativus] >KGN62250.1 hypothetical ...	[more]

BLAST of Carg20543 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIE0	8.6e-06	50.00	DNA oxidative demethylase ALKBH2 OS=Arabidopsis thaliana OX=3702 GN=ALKBH2 PE=2 ...	[more]

BLAST of Carg20543 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KPP3	3.0e-110	99.04	uncharacterized protein LOC111496478 OS=Cucurbita maxima OX=3661 GN=LOC111496478...	[more]
A0A6J1GK09	3.0e-110	99.04	uncharacterized protein LOC111454570 OS=Cucurbita moschata OX=3662 GN=LOC1114545...	[more]
A0A0A0LK08	4.6e-103	94.17	DUF4057 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G345910 PE=...	[more]
A0A5A7V9J6	1.7e-102	93.27	DUF4057 domain-containing protein OS=Cucumis melo var. makuwa OX=1194695 GN=E6C2...	[more]
A0A1S3BD79	1.7e-102	93.27	uncharacterized protein LOC103488402 OS=Cucumis melo OX=3656 GN=LOC103488402 PE=...	[more]

BLAST of Carg20543 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G35780.1	1.1e-67	67.80	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G78150.1	6.8e-67	67.33	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G78150.2	6.8e-67	67.33	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]
AT1G78150.3	3.0e-62	58.87	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT4G39860.1	6.8e-59	58.82	unknown protein; BEST Arabidopsis thaliana protein match is: unknown protein (TA...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR025131	Domain of unknown function DUF4057	PFAM	PF13266	DUF4057	coord: 34..237 e-value: 2.2E-102 score: 342.8
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..86
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 22..38
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 57..76
None	No IPR available	PANTHER	PTHR31132:SF20	SUBFAMILY NOT NAMED	coord: 32..235
None	No IPR available	PANTHER	PTHR31132	N-LYSINE METHYLTRANSFERASE	coord: 32..235

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20543-RA	Carg20543-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032259	methylation
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0008168	methyltransferase activity