Carg20539 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20539
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	melanoma-associated antigen 1
Location	Carg_Chr12: 837866 .. 841133 (+)
RNA-Seq Expression	Carg20539
Synteny	Carg20539

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

TGGAGGTTTCTGAAGCTTCTCTAAAATTCCGACATCACTTTCGCCGGAAGTTTCAAAGATTTCCCGTCGCTCTTTTCAGCGCTTGAATTCTCCTTTATATCTTATCGTCGGCTTAGTCCGGGGTAAGCTATGGAATTACTTCAGTTTTATTATCAATAGTCTCTCATGTTTTGTCAATATTGTCTATTAGAATCACTTTTCATCCTCGATCAAGCCCGACCTATCTGTTAAAATCGTTCTTGTTGATATATATATATATCGATATATAGTGATTTTGGAAATCCACCGTTGTTCTGTCATGACTTCAGTTGTTGATTTCGATTGCATATGTTCATTTGACCTCGAATTGATTTTGATTTTCGTTTTATTGTTAGGGAAATTGTTGAGTATGGCAAGTTCAGGAGATTATCTTTCCCAGATTGACATTTCGAACGAGGTATGAGATTGTTCTCTCTGCATAAAGCCCTGTATGACTATTTGGAAATCGAGGGTTTGCTCCTTGTATCGCCCTCGCATGGTTAATATTTCTGATTCCGAAACCACTTTTAATCGATAGGAAAAGGACAAGCTCGTGGCGGAAGTTATCCGCTATATCATATTCAAAACTCATCAAAATTCTGGATGTCCCATCAAAAGGGAAGAGCTTACTCAGATCGTAACAAAAAATTATAGGAATCGAGGATTGCCGGCTATTGTGATAGAAGAAGCTAAAGAGAAGCTGTCAAGCATTTTTGGTTATGAGTTGAGGGAACTTCAAAGAGCACGGCCTTCATCCACTGCCCAAGGGCACCCTCCTCAACATAGTAAGAAGTGATCTAACTTATTATCTTACTCTTGATTATTACGATCGAGATCTTCAGACGTCGGGCCAACTCCCATTTATTCATGTTATCTTACTGCAATCCTAGTTATCAGAATTTTTGTAGATATGACTACTGCAAGTCACCACAGGTTTCTTTTAACAAACAGTACAGGGTTATGGTTCAGTCTTGTTATGAGTCGAAGCATACAAATTTTAAGAATGAGCTAACCTTTTGCAACTTTAGTATAGAAAAAGTGTCCAACTAATGATCTTGTCGGTGGAACAAAACGTTCTTGTGGGTGACGCATCCATAGGGTGAGAAATTCATTTCTTCTAGTAATGTTATTGTTATTACTCCAATCCGTGAGCTGTGCACATGCTTTATATTAAATGTGGTTCATTGATCATTTTATTACTTATGCTACACACTCCTATCTCAAGAACCATATATGTGAAATAGCACGTATTCGAATTTATTCTCATCTCAGAAACTGCGTACGCCTAGGTTTTGGTTCATTCTTCAAAATAAACGTCTGATGTATCTGATATACAATCCTCTTTATTCATTGCATCGAAATAAAACAACTGGAACATGAGGGCTGGCATTTTTTTAGCATGAAAGTTGTGGCTATGGGTTGTAGAAAGTCATACTAAATTCAATGGATGTACAGACTGCTGAATGTGGTTATTTGGTTACAAATTTTCTTAGTCGCTGAATAAGCTCTCATTTCGTCAAGAACTAGGATCTGCTGTATGCTTCATGGCTTGCCATTAATATTCTAATTTTGGGGTTACTTCCATCTGACATGGCTTACTGCAAAACTATATCGTTATTGGATCTTGCTTTAACTCTTGTCACTACATGCCTTCAGATGTTGTAGATTCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCGGAGGTTTATAGAAAGTATGTTGAGGATGTCGATACTGCGCACGTGACTGGTTTTAATTTTGTTGTAGTTAGTATTGTACATCTGGCTGGAGGCAAAATTCCTGAAGGTTAGCTCTATGGCTGTAGTTTCTTAATCCAATGCATACTTGGTAGATTTAGAGTGATAGAAAGCCAGCTTACACATCAAATGGATTTCATTCATAAACTTTTTCTTTCATCTAATTTTGGAGACACTAATTGTAACTCAAGCCCACCGCTAGCAGATATTGTCCTCTTTGGGTTTTCCGTTTTGGGCTTCTCCTCAAGGTTTTACCGTTTTAAAAATCTTGAGGGGAAGCCCAAATGGGAAAGCCCAAAGAGGACAATATTTGTTAGCGGTAGGCTTGGGCCGTTACACTAATTGTACATTACCTAGAGAAGATGTTGCTTATCAGATAGTTATACTAAGTTTTATACCATATTAACTGACTAAAAAAATATTTCAACGAGTTTCTTAGAGCAAAAGTTCCAATTTTTTTGCTTCTCTTTCATCTAATCTAGTACATTACTTTGAAAAGATGTTGCTTATCAGATAGCCATGCTACATTTATGCAATATTGATCGACTCACAATGTTTCAAAGAGTTTCTTAGGGCAGAATGCCATTTTTTGCTTTATAATGGAACCTGGCAATACACTGCAGAAAGCCTTTGGCATCACTTGAGACGAATGGGGTTGTCTGAAAATGATGAAAAACATCCAGTCCTTGGCAACATCAAGCAGGCAGTAGAGTTGCTTGTCCAGCAAAGGTCAGTAAACTCTGTACTATATGCTTGGTAACTGCAATTGAGCAACTTCAACATAATTCGATGACCATAGAAGATACTGGAAAGACGGGTTTACAGGTTCATCTTATGCTTGCCGTGCTTTGTAGATATCTGCAGAAGGCCAAAGTAAATGGTTCTGAAGGGAATATCACGTTCTACGAGCTTGCTGAAAGAGCTATAGATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGGTATGCAGCTTGTCTATATGTTTATATAGTGATGGATGAATATGTTGTTTTTAGTACAAATAAATGATAATCTTGTAGCATAAAATCTCATAAAGTTGCTAAATTTAAACTTATTATTTGCTTACTTTGGAAGAAGATTAAATGAACACGAGGAATAGAATCCCATAATCGTATTGTCGTGCATTTTAGATCATGTGCCGAAAAATATACAGGATGTCTATTAATAATTAGTTTTGCGTAATTTCTTAAGTAGCATAAAAATTGTGCTTAATATGGATGGTTGGATGGACGAATCAATGCTACCATGAATGATGGAGCCCATCTTCTGAAGGATTCGCTGTTTATCTGTCTGGAGTGATATCTGTCTGGTTTCTGTTGAATCTAGTTTGCTTTCAGCCTATCATTGCATGTTTCTAATAATTGTGCTTCGACAGCATCAGATCATAGCTTCACGCATTCGACTTAGTTTCTCCTATTCCAAGCTGTCTTTTGCTGCTACAGAATGTCCAAGCATATTATAA

mRNA sequence

TGGAGGTTTCTGAAGCTTCTCTAAAATTCCGACATCACTTTCGCCGGAAGTTTCAAAGATTTCCCGTCGCTCTTTTCAGCGCTTGAATTCTCCTTTATATCTTATCGTCGGCTTAGTCCGGGGGAAATTGTTGAGTATGGCAAGTTCAGGAGATTATCTTTCCCAGATTGACATTTCGAACGAGGAAAAGGACAAGCTCGTGGCGGAAGTTATCCGCTATATCATATTCAAAACTCATCAAAATTCTGGATGTCCCATCAAAAGGGAAGAGCTTACTCAGATCGTAACAAAAAATTATAGGAATCGAGGATTGCCGGCTATTGTGATAGAAGAAGCTAAAGAGAAGCTGTCAAGCATTTTTGGTTATGAGTTGAGGGAACTTCAAAGAGCACGGCCTTCATCCACTGCCCAAGGGCACCCTCCTCAACATAATGTTGTAGATTCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCGGAGGTTTATAGAAAGTATGTTGAGGATGTCGATACTGCGCACGTGACTGGTTTTAATTTTGTTGTAGTTAGTATTGTACATCTGGCTGGAGGCAAAATTCCTGAAGAAAGCCTTTGGCATCACTTGAGACGAATGGGGTTGTCTGAAAATGATGAAAAACATCCAGTCCTTGGCAACATCAAGCAGGCAGTAGAGTTGCTTGTCCAGCAAAGATATCTGCAGAAGGCCAAAGTAAATGGTTCTGAAGGGAATATCACGTTCTACGAGCTTGCTGAAAGAGCTATAGATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGCATCAGATCATAGCTTCACGCATTCGACTTAGTTTCTCCTATTCCAAGCTGTCTTTTGCTGCTACAGAATGTCCAAGCATATTATAA

Coding sequence (CDS)

ATGGCAAGTTCAGGAGATTATCTTTCCCAGATTGACATTTCGAACGAGGAAAAGGACAAGCTCGTGGCGGAAGTTATCCGCTATATCATATTCAAAACTCATCAAAATTCTGGATGTCCCATCAAAAGGGAAGAGCTTACTCAGATCGTAACAAAAAATTATAGGAATCGAGGATTGCCGGCTATTGTGATAGAAGAAGCTAAAGAGAAGCTGTCAAGCATTTTTGGTTATGAGTTGAGGGAACTTCAAAGAGCACGGCCTTCATCCACTGCCCAAGGGCACCCTCCTCAACATAATGTTGTAGATTCAAAATCCTATGTTCTCATAAGCAAGCTTCCTGCGGAGGTTTATAGAAAGTATGTTGAGGATGTCGATACTGCGCACGTGACTGGTTTTAATTTTGTTGTAGTTAGTATTGTACATCTGGCTGGAGGCAAAATTCCTGAAGAAAGCCTTTGGCATCACTTGAGACGAATGGGGTTGTCTGAAAATGATGAAAAACATCCAGTCCTTGGCAACATCAAGCAGGCAGTAGAGTTGCTTGTCCAGCAAAGATATCTGCAGAAGGCCAAAGTAAATGGTTCTGAAGGGAATATCACGTTCTACGAGCTTGCTGAAAGAGCTATAGATGGACCAGTTAGTGAAAAGATTAAAGAATATGTAGCACAGCATCAGATCATAGCTTCACGCATTCGACTTAGTTTCTCCTATTCCAAGCTGTCTTTTGCTGCTACAGAATGTCCAAGCATATTATAA

Protein sequence

MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVELLVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQHQIIASRIRLSFSYSKLSFAATECPSIL

Homology

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BLAST of Carg20539 vs. NCBI nr
Match: KAG7020215.1 (Melanoma-associated antigen 8 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 495.4 bits (1274), Expect = 3.0e-136
Identity = 251/251 (100.00%), Postives = 251/251 (100.00%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQHQIIASRIRLSFSYSKL 240
           LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQHQIIASRIRLSFSYSKL
Sbjct: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQHQIIASRIRLSFSYSKL 240

Query: 241 SFAATECPSIL 252
           SFAATECPSIL
Sbjct: 241 SFAATECPSIL 251

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BLAST of Carg20539 vs. NCBI nr
Match: KAG6585294.1 (Non-structural maintenance of chromosomes element 3-like protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 443.4 bits (1139), Expect = 1.3e-120
Identity = 223/223 (100.00%), Postives = 223/223 (100.00%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 553 MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 612

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 613 AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 672

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL
Sbjct: 673 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 732

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 733 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 775

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BLAST of Carg20539 vs. NCBI nr
Match: XP_023002674.1 (melanoma-associated antigen 1 [Cucurbita maxima] >XP_023002675.1 melanoma-associated antigen 1 [Cucurbita maxima])

HSP 1 Score: 441.0 bits (1133), Expect = 6.7e-120
Identity = 221/223 (99.10%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQQRYLQKAK+NG EGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223

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BLAST of Carg20539 vs. NCBI nr
Match: XP_022952014.1 (melanoma-associated antigen 2 [Cucurbita moschata] >XP_022952015.1 melanoma-associated antigen 2 [Cucurbita moschata])

HSP 1 Score: 438.7 bits (1127), Expect = 3.3e-119
Identity = 219/223 (98.21%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIK+AVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKEAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQQRYLQKAK+NG +GNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPDGNITFYELAERAIDGPVSEKIKEYVAQ 223

[Close]

BLAST of Carg20539 vs. NCBI nr
Match: XP_023538088.1 (melanoma-associated antigen 1 [Cucurbita pepo subsp. pepo] >XP_023538089.1 melanoma-associated antigen 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 436.8 bits (1122), Expect = 1.3e-118
Identity = 219/223 (98.21%), Postives = 221/223 (99.10%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQQRYLQK K+NG EGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKDKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223

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BLAST of Carg20539 vs. ExPASy Swiss-Prot
Match: P43361 (Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2)

HSP 1 Score: 71.6 bits (174), Expect = 1.4e-11
Identity = 60/221 (27.15%), Postives = 110/221 (49.77%), Query Frame = 0


Query: 16  EEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIF 75
           E  D+ VAE++R+++ K       P+ + E+ + V KNY+N   P I   +A E +  IF
Sbjct: 110 EALDEKVAELVRFLLRKYQIKE--PVTKAEMLESVIKNYKNH-FPDI-FSKASECMQVIF 169

Query: 76  GYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFV 135
           G +++E+  A       GH          SY+L++ L    Y   + D  +   TG   +
Sbjct: 170 GIDVKEVDPA-------GH----------SYILVTCLGLS-YDGLLGDDQSTPKTGLLII 229

Query: 136 VVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAV-ELLVQQRYLQKAKVNG 195
           V+ ++ + G + PEE++W  L  MGL +  E H V   +++ + +  VQ+ YL+  +  G
Sbjct: 230 VLGMILMEGSRAPEEAIWEALSVMGLYDGRE-HSVYWKLRKLLTQEWVQENYLEYRQAPG 289

Query: 196 SEGNITFYELAERAIDGPVSEKIKEYVAQHQIIASRIRLSF 236
           S+     +    RA+      K+ E+V +   + +R+R+S+
Sbjct: 290 SDPVRYEFLWGPRALAETSYVKVLEHVVR---VNARVRISY 304

[Close]

BLAST of Carg20539 vs. ExPASy Swiss-Prot
Match: P43363 (Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2)

HSP 1 Score: 66.6 bits (161), Expect = 4.5e-10
Identity = 50/185 (27.03%), Postives = 92/185 (49.73%), Query Frame = 0


Query: 13  ISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLS 72
           +   E D+ V +++++++FK       PI + E+ + V +NY +     ++  EA E + 
Sbjct: 129 LPRSEIDEKVTDLVQFLLFKYQMKE--PITKAEILESVIRNYEDHF--PLLFSEASECML 188

Query: 73  SIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGF 132
            +FG +++E+            P  H      S+VL++ L    Y   + DV +   TG 
Sbjct: 189 LVFGIDVKEVD-----------PTGH------SFVLVTSL-GLTYDGMLSDVQSMPKTGI 248

Query: 133 NFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAV-ELLVQQRYLQKAK 192
             +++SIV + G   PEE +W  L  MGL +  E H + G  ++ + +  VQ+ YL+  +
Sbjct: 249 LILILSIVFIEGYCTPEEVIWEALNMMGLYDGME-HLIYGEPRKLLTQDWVQENYLEYRQ 290

Query: 193 VNGSE 197
           V GS+
Sbjct: 309 VPGSD 290

[Close]

BLAST of Carg20539 vs. ExPASy Swiss-Prot
Match: P43355 (Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1)

HSP 1 Score: 65.9 bits (159), Expect = 7.7e-10
Identity = 60/217 (27.65%), Postives = 106/217 (48.85%), Query Frame = 0


Query: 20  KLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYEL 79
           K VA+++ +++ K       P+ + E+ + V KNY++   P I   +A E L  +FG ++
Sbjct: 104 KKVADLVGFLLLKYRARE--PVTKAEMLESVIKNYKH-CFPEI-FGKASESLQLVFGIDV 163

Query: 80  RELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVSI 139
           +E   A P+    GH          SYVL++ L    Y   + D      TGF  +V+ +
Sbjct: 164 KE---ADPT----GH----------SYVLVTCLGLS-YDGLLGDNQIMPKTGFLIIVLVM 223

Query: 140 VHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAV-ELLVQQRYLQKAKVNGSEGN 199
           + + GG  PEE +W  L  M + +  E H   G  ++ + + LVQ++YL+  +V  S+  
Sbjct: 224 IAMEGGHAPEEEIWEELSVMEVYDGRE-HSAYGEPRKLLTQDLVQEKYLEYRQVPDSDPA 283

Query: 200 ITFYELAERAIDGPVSEKIKEYVAQHQIIASRIRLSF 236
              +    RA+      K+ EYV +   +++R+R  F
Sbjct: 284 RYEFLWGPRALAETSYVKVLEYVIK---VSARVRFFF 294

[Close]

BLAST of Carg20539 vs. ExPASy Swiss-Prot
Match: P43356 (Melanoma-associated antigen 2 OS=Homo sapiens OX=9606 GN=MAGEA2 PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 6.7e-06
Identity = 41/175 (23.43%), Postives = 78/175 (44.57%), Query Frame = 0


Query: 22  VAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYELRE 81
           + E++ +++ K       P+ + E+ + V +N ++     ++  +A E L  +FG E+ E
Sbjct: 113 MVELVHFLLLKYRARE--PVTKAEMLESVLRNCQD--FFPVIFSKASEYLQLVFGIEVVE 172

Query: 82  LQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVSIVH 141
           +                 V  S  Y+L++ L    Y   + D      TG   +V++I+ 
Sbjct: 173 V-----------------VPISHLYILVTCLGLS-YDGLLGDNQVMPKTGLLIIVLAIIA 232

Query: 142 LAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVELLVQQRYLQKAKVNGSE 197
           + G   PEE +W  L  + + E  E        K  ++ LVQ+ YL+  +V GS+
Sbjct: 233 IEGDCAPEEKIWEELSMLEVFEGREDSVFAHPRKLLMQDLVQENYLEYRQVPGSD 265

[Close]

BLAST of Carg20539 vs. ExPASy Swiss-Prot
Match: P43365 (Melanoma-associated antigen 12 OS=Homo sapiens OX=9606 GN=MAGEA12 PE=1 SV=2)

HSP 1 Score: 52.0 bits (123), Expect = 1.1e-05
Identity = 43/175 (24.57%), Postives = 77/175 (44.00%), Query Frame = 0


Query: 22  VAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLPAIVIEEAKEKLSSIFGYELRE 81
           +AE++ +++ K       P  + E+   V +N+++     ++  +A E L  +FG E+ E
Sbjct: 113 MAELVHFLLLKYRARE--PFTKAEMLGSVIRNFQD--FFPVIFSKASEYLQLVFGIEVVE 172

Query: 82  LQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKYVEDVDTAHVTGFNFVVVSIVH 141
           + R        GH           Y+L++ L    Y   + D      TG   +V++I+ 
Sbjct: 173 VVRI-------GH----------LYILVTCLGLS-YDGLLGDNQIVPKTGLLIIVLAIIA 232

Query: 142 LAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVELLVQQRYLQKAKVNGSE 197
             G   PEE +W  L  +  S+  E        K   + LVQ+ YL+  +V GS+
Sbjct: 233 KEGDCAPEEKIWEELSVLEASDGREDSVFAHPRKLLTQDLVQENYLEYRQVPGSD 265

[Close]

BLAST of Carg20539 vs. ExPASy TrEMBL
Match: A0A6J1KPM1 (melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 SV=1)

HSP 1 Score: 441.0 bits (1133), Expect = 3.2e-120
Identity = 221/223 (99.10%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQQRYLQKAK+NG EGNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPEGNITFYELAERAIDGPVSEKIKEYVAQ 223

[Close]

BLAST of Carg20539 vs. ExPASy TrEMBL
Match: A0A6J1GKJ9 (melanoma-associated antigen 2 OS=Cucurbita moschata OX=3662 GN=LOC111454728 PE=4 SV=1)

HSP 1 Score: 438.7 bits (1127), Expect = 1.6e-119
Identity = 219/223 (98.21%), Postives = 222/223 (99.55%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP
Sbjct: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY
Sbjct: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIK+AVEL
Sbjct: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKEAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQQRYLQKAK+NG +GNITFYELAERAIDGPVSEKIKEYVAQ
Sbjct: 181 LVQQRYLQKAKINGPDGNITFYELAERAIDGPVSEKIKEYVAQ 223

[Close]

BLAST of Carg20539 vs. ExPASy TrEMBL
Match: A0A6J1BU01 (melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=4 SV=1)

HSP 1 Score: 408.7 bits (1049), Expect = 1.8e-110
Identity = 200/223 (89.69%), Postives = 214/223 (95.96%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MA +GDYLSQIDISNEEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MAGAGDYLSQIDISNEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           A+VIEEAKEK+SSIFGYELRELQR RPSST+QGH  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AVVIEEAKEKMSSIFGYELRELQRTRPSSTSQGHSSQQNVADTKSYVLISKLPADIYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDV+TAHVTGF FVVVSIVHLAGGKIPEESLWHHLRRMGLSE+DEKHPVLGNIKQAVEL
Sbjct: 121 VEDVNTAHVTGFTFVVVSIVHLAGGKIPEESLWHHLRRMGLSESDEKHPVLGNIKQAVEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           LVQ+RYLQKAK+NG EGNITFYELAERA+DGPVSE+IKEYVAQ
Sbjct: 181 LVQKRYLQKAKINGPEGNITFYELAERALDGPVSEEIKEYVAQ 223

[Close]

BLAST of Carg20539 vs. ExPASy TrEMBL
Match: A0A1S3BBA9 (uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=4 SV=1)

HSP 1 Score: 387.9 bits (995), Expect = 3.3e-104
Identity = 193/223 (86.55%), Postives = 205/223 (91.93%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MAS+G++LSQ+DIS EEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MASAGEHLSQLDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVI+EAK KLSSIFGYELRELQR RPSST Q H  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVADAKSYVLISKLPADIYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDVDTAHVTGF FVVVSIVHLAGGKIPEESL HHLRRMGLSE+DE HPVLGNIK A+EL
Sbjct: 121 VEDVDTAHVTGFTFVVVSIVHLAGGKIPEESLRHHLRRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           +VQQRYLQK KVN  EGNITFYELAERA DGPVSEKIKEYVAQ
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQ 223

[Close]

BLAST of Carg20539 vs. ExPASy TrEMBL
Match: A0A0A0LL83 (MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.7e-100
Identity = 187/223 (83.86%), Postives = 203/223 (91.03%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MAS+G+Y  ++DIS EEKDKLVAE IRYIIFKTHQNSGCPIKREELTQ+VTKNYRNRGLP
Sbjct: 1   MASAGEY--ELDISEEEKDKLVAEAIRYIIFKTHQNSGCPIKREELTQLVTKNYRNRGLP 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQHNVVDSKSYVLISKLPAEVYRKY 120
           AIVI+EAK KLSSIFGYELRELQR RPSST Q H  Q NV D+KSYVLISKLPA++YRKY
Sbjct: 61  AIVIDEAKRKLSSIFGYELRELQRTRPSSTGQPHSSQQNVGDAKSYVLISKLPADIYRKY 120

Query: 121 VEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVEL 180
           VEDV+TAHVTGF FV++SIVHLAGGKIPEESL HHL+RMGLSE+DE HPVLGNIK A+EL
Sbjct: 121 VEDVNTAHVTGFTFVIISIVHLAGGKIPEESLRHHLKRMGLSESDENHPVLGNIKHAIEL 180

Query: 181 LVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
           +VQQRYLQK KVN  EGNITFYELAERA DGPVSEKIKEYVAQ
Sbjct: 181 VVQQRYLQKVKVNAPEGNITFYELAERATDGPVSEKIKEYVAQ 221

[Close]

BLAST of Carg20539 vs. TAIR 10
Match: AT1G34770.1 (CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hits to 1260 proteins in 85 species: Archae - 0; Bacteria - 0; Metazoa - 1104; Fungi - 45; Plants - 49; Viruses - 0; Other Eukaryotes - 76 (source: NCBI BLink). )

HSP 1 Score: 297.7 bits (761), Expect = 8.5e-81
Identity = 150/224 (66.96%), Postives = 182/224 (81.25%), Query Frame = 0


Query: 1   MASSGDYLSQIDISNEEKDKLVAEVIRYIIFKTHQNSGCPIKREELTQIVTKNYRNRGLP 60
           MA   D LSQ DIS EE DKLV+EVIR+I+FK HQ+SG PIKRE+LTQIVTKNYR R L 
Sbjct: 1   MADEEDSLSQFDISKEETDKLVSEVIRFILFKFHQSSGTPIKREDLTQIVTKNYRQRNLA 60

Query: 61  AIVIEEAKEKLSSIFGYELRELQRARPSSTAQGHPPQ-HNVVDSKSYVLISKLPAEVYRK 120
             VI EAK+KLS++FGY+L+ELQRAR SST Q   PQ  + VDSKSYVL+S+LP EV++K
Sbjct: 61  THVINEAKKKLSNVFGYDLKELQRARSSSTGQSRLPQSQSSVDSKSYVLVSELPLEVFKK 120

Query: 121 YVEDVDTAHVTGFNFVVVSIVHLAGGKIPEESLWHHLRRMGLSENDEKHPVLGNIKQAVE 180
           +V D  T+ VTGF FVV++IV LAGGKIPEE+LWHHL+RMGL ENDE +PV GN KQ +E
Sbjct: 121 HVVDETTSPVTGFTFVVLAIVQLAGGKIPEETLWHHLKRMGLHENDEHNPVFGNNKQTLE 180

Query: 181 LLVQQRYLQKAKVNGSEGNITFYELAERAIDGPVSEKIKEYVAQ 224
            LVQQR+LQK KV+G EG+  FY+LAERA+D  VSEK+K+Y++Q
Sbjct: 181 TLVQQRFLQKEKVSGPEGSTLFYDLAERALDPQVSEKVKDYISQ 224

The following BLAST results are available for this feature:

BLAST of Carg20539 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020215.1	3.0e-136	100.00	Melanoma-associated antigen 8 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6585294.1	1.3e-120	100.00	Non-structural maintenance of chromosomes element 3-like protein, partial [Cucur...	[more]
XP_023002674.1	6.7e-120	99.10	melanoma-associated antigen 1 [Cucurbita maxima] >XP_023002675.1 melanoma-associ...	[more]
XP_022952014.1	3.3e-119	98.21	melanoma-associated antigen 2 [Cucurbita moschata] >XP_022952015.1 melanoma-asso...	[more]
XP_023538088.1	1.3e-118	98.21	melanoma-associated antigen 1 [Cucurbita pepo subsp. pepo] >XP_023538089.1 melan...	[more]

BLAST of Carg20539 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P43361	1.4e-11	27.15	Melanoma-associated antigen 8 OS=Homo sapiens OX=9606 GN=MAGEA8 PE=1 SV=2	[more]
P43363	4.5e-10	27.03	Melanoma-associated antigen 10 OS=Homo sapiens OX=9606 GN=MAGEA10 PE=2 SV=2	[more]
P43355	7.7e-10	27.65	Melanoma-associated antigen 1 OS=Homo sapiens OX=9606 GN=MAGEA1 PE=1 SV=1	[more]
P43356	6.7e-06	23.43	Melanoma-associated antigen 2 OS=Homo sapiens OX=9606 GN=MAGEA2 PE=1 SV=1	[more]
P43365	1.1e-05	24.57	Melanoma-associated antigen 12 OS=Homo sapiens OX=9606 GN=MAGEA12 PE=1 SV=2	[more]

BLAST of Carg20539 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KPM1	3.2e-120	99.10	melanoma-associated antigen 1 OS=Cucurbita maxima OX=3661 GN=LOC111496460 PE=4 S...	[more]
A0A6J1GKJ9	1.6e-119	98.21	melanoma-associated antigen 2 OS=Cucurbita moschata OX=3662 GN=LOC111454728 PE=4...	[more]
A0A6J1BU01	1.8e-110	89.69	melanoma-associated antigen 1 OS=Momordica charantia OX=3673 GN=LOC111004771 PE=...	[more]
A0A1S3BBA9	3.3e-104	86.55	uncharacterized protein LOC103488065 OS=Cucumis melo OX=3656 GN=LOC103488065 PE=...	[more]
A0A0A0LL83	1.7e-100	83.86	MAGE domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368960 PE=4 S...	[more]

BLAST of Carg20539 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G34770.1	8.5e-81	66.96	CONTAINS InterPro DOMAIN/s: MAGE protein (InterPro:IPR002190); Has 1274 Blast hi...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002190	MAGE homology domain	SMART	SM01373	MAGE_2	coord: 25..217 e-value: 1.4E-32 score: 124.3
IPR002190	MAGE homology domain	PFAM	PF01454	MAGE	coord: 25..208 e-value: 5.8E-39 score: 134.0
IPR002190	MAGE homology domain	PROSITE	PS50838	MAGE	coord: 18..223 score: 16.028111
IPR041899	MAGE homology domain, winged helix WH2 motif	GENE3D	1.10.10.1210	MAGE homology domain, winged helix WH2 motif	coord: 118..232 e-value: 2.9E-19 score: 71.0
IPR041898	MAGE homology domain, winged helix WH1 motif	GENE3D	1.10.10.1200	MAGE homology domain, winged helix WH1 motif	coord: 12..114 e-value: 1.2E-23 score: 84.8
None	No IPR available	PANTHER	PTHR11736:SF14	MAGE PROTEIN	coord: 4..224
IPR037445	Melanoma-associated antigen	PANTHER	PTHR11736	MELANOMA-ASSOCIATED ANTIGEN MAGE ANTIGEN	coord: 4..224

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20539-RA	Carg20539-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0005634	nucleus