Carg20536 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20536
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	RNA-binding protein 38-like
Location	Carg_Chr12: 860914 .. 861807 (+)
RNA-Seq Expression	Carg20536
Synteny	Carg20536

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGATCATTTCTCCAATCCCCCGCCGCTCTTCGGCGACACCACATGGACCAAAGTCTTCGTCGGCGGCCTCGCTTGGGAGACCAAATCCGACGAAATGCACAGATTCTTCCAACAGTTCGGCGAGATTCTCGAAGCCGTCATAATCAAAGATAAAACCACCGGAAAATCCAAAGGCTACGGATTCGTAAGTTCCTTATTCCTCTCCGATTCCATTTCCGGTTACGATTTTAATTCCGCCGTCTCCGATTTTGTAATTAGGTCACTTTCCGAGATCCTCAATCGGCGAGAATGGCCTGCGCTAATCCAAATCCGATTATCTGTGGAAGACGAGCAAACTGTAACATCGCCGTCTTCGGTCCGCCTCGTCCGCCGCCGTCCGGAGGTAACTTTCTTAATCTCATTACCGGAAAACGATCGATTGATTTCTGTTAACATTCTTCTCGATTTTACAGGGAGAATCCAAACTACAGCGTCGTACGGCGGGTTACGGCCGTCGTTTCCGCCGCCGCCGGTCATTTTCCCGCCATACAGGTACATGATTCTTCGATGGCGACAATGTTACAAGGAACCAACAATTTACCTCGACAGGAAGTCAAATGTTTTTTGTTTTTTTGTTTCTTTTTGCAGATACCGATCCTACGCCCCAAATTACACAGTACCTTATGGCTATGGCTATGGCATTGGATACGGCCATGGCTATGGCTATGGTCATGGCTATGGCTATGGCTATGACTGTTCTTCATCATCACCGGCTCCAAGAGCGCCATTTTCATCACCATCATCAGCGGCAGCACCCTATGTTCCGTACTATATGAATCAGGGGTTGTTCTACTCTCCGATGCACCAGATTATTACTCCCAGTACTGGTAATTATTTATCGTTCTATTAA

mRNA sequence

ATGGCCGATCATTTCTCCAATCCCCCGCCGCTCTTCGGCGACACCACATGGACCAAAGTCTTCGTCGGCGGCCTCGCTTGGGAGACCAAATCCGACGAAATGCACAGATTCTTCCAACAGTTCGGCGAGATTCTCGAAGCCGTCATAATCAAAGATAAAACCACCGGAAAATCCAAAGGCTACGGATTCGTAAGTTCCTTATTCCTCTCCGATTCCATTTCCGGTTACGATTTTAATTCCGCCGTCTCCGATTTTGTAATTAGGTCACTTTCCGAGATCCTCAATCGGCGAGAATGGCCTGCGCTAATCCAAATCCGATTATCTGTGGAAGACGAGCAAACTGTAACATCGCCGTCTTCGGTCCGCCTCGTCCGCCGCCGTCCGGAGGTAACTTTCTTAATCTCATTACCGGAAAACGATCGATTGATTTCTGTTAACATTCTTCTCGATTTTACAGGGAGAATCCAAACTACAGCGTCGTACGGCGGGTTACGGCCGTCGTTTCCGCCGCCGCCGGTCATTTTCCCGCCATACAGATACCGATCCTACGCCCCAAATTACACAGTACCTTATGGCTATGGCTATGGCATTGGATACGGCCATGGCTATGGCTATGGTCATGGCTATGGCTATGGCTATGACTGTTCTTCATCATCACCGGCTCCAAGAGCGCCATTTTCATCACCATCATCAGCGGCAGCACCCTATGTTCCGTACTATATGAATCAGGGGTTGTTCTACTCTCCGATGCACCAGATTATTACTCCCAGTACTGGTAATTATTTATCGTTCTATTAA

Coding sequence (CDS)

ATGGCCGATCATTTCTCCAATCCCCCGCCGCTCTTCGGCGACACCACATGGACCAAAGTCTTCGTCGGCGGCCTCGCTTGGGAGACCAAATCCGACGAAATGCACAGATTCTTCCAACAGTTCGGCGAGATTCTCGAAGCCGTCATAATCAAAGATAAAACCACCGGAAAATCCAAAGGCTACGGATTCGTAAGTTCCTTATTCCTCTCCGATTCCATTTCCGGTTACGATTTTAATTCCGCCGTCTCCGATTTTGTAATTAGGTCACTTTCCGAGATCCTCAATCGGCGAGAATGGCCTGCGCTAATCCAAATCCGATTATCTGTGGAAGACGAGCAAACTGTAACATCGCCGTCTTCGGTCCGCCTCGTCCGCCGCCGTCCGGAGGTAACTTTCTTAATCTCATTACCGGAAAACGATCGATTGATTTCTGTTAACATTCTTCTCGATTTTACAGGGAGAATCCAAACTACAGCGTCGTACGGCGGGTTACGGCCGTCGTTTCCGCCGCCGCCGGTCATTTTCCCGCCATACAGATACCGATCCTACGCCCCAAATTACACAGTACCTTATGGCTATGGCTATGGCATTGGATACGGCCATGGCTATGGCTATGGTCATGGCTATGGCTATGGCTATGACTGTTCTTCATCATCACCGGCTCCAAGAGCGCCATTTTCATCACCATCATCAGCGGCAGCACCCTATGTTCCGTACTATATGAATCAGGGGTTGTTCTACTCTCCGATGCACCAGATTATTACTCCCAGTACTGGTAATTATTTATCGTTCTATTAA

Protein sequence

MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSSVRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTASYGGLRPSFPPPPVIFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYYMNQGLFYSPMHQIITPSTGNYLSFY

Homology

[Close]

BLAST of Carg20536 vs. NCBI nr
Match: KAG7020218.1 (putative RNA-binding protein ARP1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 535.8 bits (1379), Expect = 2.1e-148
Identity = 265/265 (100.00%), Postives = 265/265 (100.00%), Query Frame = 0


Query: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60
           MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG
Sbjct: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60

Query: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120
           YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS
Sbjct: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120

Query: 121 VRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTASYGGLRPSFPPPPVIFPPYRY 180
           VRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTASYGGLRPSFPPPPVIFPPYRY
Sbjct: 121 VRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTASYGGLRPSFPPPPVIFPPYRY 180

Query: 181 RSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYY 240
           RSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYY
Sbjct: 181 RSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYY 240

Query: 241 MNQGLFYSPMHQIITPSTGNYLSFY 266
           MNQGLFYSPMHQIITPSTGNYLSFY
Sbjct: 241 MNQGLFYSPMHQIITPSTGNYLSFY 265

[Close]

BLAST of Carg20536 vs. NCBI nr
Match: XP_023538118.1 (RNA-binding protein 38-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 334.3 bits (856), Expect = 9.3e-88
Identity = 179/266 (67.29%), Postives = 182/266 (68.42%), Query Frame = 0


Query: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60
           MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG
Sbjct: 24  MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 83

Query: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120
           YGFV                                                 T   P S
Sbjct: 84  YGFV-------------------------------------------------TFRDPQS 143

Query: 121 VRLVRRRPEVTFLISLPENDRLISVNILL-------DFTGRIQTTASYGGLRPSFPPPPV 180
            R+    P            R  + NI +          GRIQTTASYGGLRPSFPPPPV
Sbjct: 144 ARMACANPNPIIC------GRRANCNIAVFGPPRPPPSGGRIQTTASYGGLRPSFPPPPV 203

Query: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 240
           IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA
Sbjct: 204 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 234

Query: 241 APYVPYYMNQGLFYSPMHQIITPSTG 260
           APYVPYYMNQGLFYSPMHQIITPSTG
Sbjct: 264 APYVPYYMNQGLFYSPMHQIITPSTG 234

[Close]

BLAST of Carg20536 vs. NCBI nr
Match: XP_022951640.1 (RNA-binding protein 38-like [Cucurbita moschata])

HSP 1 Score: 329.7 bits (844), Expect = 2.3e-86
Identity = 179/267 (67.04%), Postives = 182/267 (68.16%), Query Frame = 0


Query: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60
           MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG
Sbjct: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60

Query: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120
           YGFV                                                 T   P S
Sbjct: 61  YGFV-------------------------------------------------TFRDPQS 120

Query: 121 VRLVRRRPEVTFLISLPENDRLISVNILL-------DFTGRIQTTASYGGLRPSFPPPPV 180
            R+    P            R  + NI +          GRIQTTASYGGLRPSFPPPPV
Sbjct: 121 ARMACANPNPIIC------GRRANCNIAVFGPPRPPPSGGRIQTTASYGGLRPSFPPPPV 180

Query: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 240
           IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA
Sbjct: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 212

Query: 241 APYVPYYMN-QGLFYSPMHQIITPSTG 260
           APYVPYYMN QGLFYSPMHQIITPSTG
Sbjct: 241 APYVPYYMNQQGLFYSPMHQIITPSTG 212

[Close]

BLAST of Carg20536 vs. NCBI nr
Match: XP_023002673.1 (RNA-binding protein 38-like [Cucurbita maxima])

HSP 1 Score: 324.3 bits (830), Expect = 9.6e-85
Identity = 174/266 (65.41%), Postives = 179/266 (67.29%), Query Frame = 0


Query: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60
           MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDK TGKSKG
Sbjct: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKITGKSKG 60

Query: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120
           YGFV                                                 T   P S
Sbjct: 61  YGFV-------------------------------------------------TFRDPQS 120

Query: 121 VRLVRRRPEVTFLISLPENDRLISVNILL-------DFTGRIQTTASYGGLRPSFPPPPV 180
            R+    P            R  + NI +          GRIQ+TASYGG+RPSFPPPPV
Sbjct: 121 ARMACANPNPIIC------GRRANCNIAVFGPPRPPPSGGRIQSTASYGGIRPSFPPPPV 180

Query: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 240
           IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA
Sbjct: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 211

Query: 241 APYVPYYMNQGLFYSPMHQIITPSTG 260
           APYVPYYMNQGLFYS MHQII PSTG
Sbjct: 241 APYVPYYMNQGLFYSQMHQIIPPSTG 211

[Close]

BLAST of Carg20536 vs. NCBI nr
Match: KAG6585298.1 (putative RNA-binding protein ARP1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 283.9 bits (725), Expect = 1.4e-72
Identity = 157/244 (64.34%), Postives = 160/244 (65.57%), Query Frame = 0


Query: 23  GGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVSSLFLSDSISGYDFNSAV 82
           GGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFV                  
Sbjct: 7   GGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFV------------------ 66

Query: 83  SDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSSVRLVRRRPEVTFLISLPENDRL 142
                                          T   P S R+    P            R 
Sbjct: 67  -------------------------------TFRDPQSARMACANPNPIIC------GRR 126

Query: 143 ISVNILL-------DFTGRIQTTASYGGLRPSFPPPPVIFPPYRYRSYAPNYTVPYGYGY 202
            + NI +          GRIQTTASYGGLRPSFPPPPVIFPPYRYRSYAPNYTVPYGYGY
Sbjct: 127 ANCNIAVFGPPRPPPSGGRIQTTASYGGLRPSFPPPPVIFPPYRYRSYAPNYTVPYGYGY 186

Query: 203 GIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYYMNQGLFYSPMHQIIT 260
           GIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYYMNQGLFYSPMHQIIT
Sbjct: 187 GIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYYMNQGLFYSPMHQIIT 195

[Close]

BLAST of Carg20536 vs. ExPASy Swiss-Prot
Match: Q9M1S3 (Probable RNA-binding protein ARP1 OS=Arabidopsis thaliana OX=3702 GN=ARP1 PE=2 SV=1)

HSP 1 Score: 76.6 bits (187), Expect = 4.6e-13
Identity = 35/54 (64.81%), Postives = 43/54 (79.63%), Query Frame = 0


Query: 12 FGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          FGDT  TKVFVGGLAW+T  + M+  F ++G+ILEAVII DK T +SKGYGFV+
Sbjct: 11 FGDTKLTKVFVGGLAWDTHKEAMYDHFIKYGDILEAVIISDKLTRRSKGYGFVT 64

[Close]

BLAST of Carg20536 vs. ExPASy Swiss-Prot
Match: Q76LC6 (RNA-binding protein 24 OS=Danio rerio OX=7955 GN=rbm24 PE=2 SV=1)

HSP 1 Score: 71.6 bits (174), Expect = 1.5e-11
Identity = 30/52 (57.69%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 14 DTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          DTT+TK+FVGGL + T    + ++F+ FGEI EAV+I D+ TGKS+GYGFV+
Sbjct: 7  DTTYTKIFVGGLPYHTTDSSLRKYFEVFGEIEEAVVITDRQTGKSRGYGFVT 58

[Close]

BLAST of Carg20536 vs. ExPASy Swiss-Prot
Match: Q9BX46 (RNA-binding protein 24 OS=Homo sapiens OX=9606 GN=RBM24 PE=1 SV=1)

HSP 1 Score: 70.9 bits (172), Expect = 2.5e-11
Identity = 30/52 (57.69%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 14 DTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          DTT+TK+FVGGL + T    + ++F+ FGEI EAV+I D+ TGKS+GYGFV+
Sbjct: 7  DTTYTKIFVGGLPYHTTDASLRKYFEVFGEIEEAVVITDRQTGKSRGYGFVT 58

[Close]

BLAST of Carg20536 vs. ExPASy Swiss-Prot
Match: Q6GQD3 (RNA-binding protein 24-A OS=Xenopus laevis OX=8355 GN=rbm24-a PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-11
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 14 DTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          DTT+TK+FVGGL + T    + ++F+ FG+I EAV+I D+ TGKS+GYGFV+
Sbjct: 7  DTTYTKIFVGGLPYHTTDSSLRKYFEVFGDIEEAVVITDRQTGKSRGYGFVT 58

[Close]

BLAST of Carg20536 vs. ExPASy Swiss-Prot
Match: Q9I8B3 (RNA-binding protein 24-B OS=Xenopus laevis OX=8355 GN=rbm24-b PE=2 SV=1)

HSP 1 Score: 70.5 bits (171), Expect = 3.3e-11
Identity = 29/52 (55.77%), Postives = 41/52 (78.85%), Query Frame = 0


Query: 14 DTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          DTT+TK+FVGGL + T    + ++F+ FG+I EAV+I D+ TGKS+GYGFV+
Sbjct: 7  DTTYTKIFVGGLPYHTTDSSLRKYFEVFGDIEEAVVITDRQTGKSRGYGFVT 58

[Close]

BLAST of Carg20536 vs. ExPASy TrEMBL
Match: A0A6J1GJE8 (RNA-binding protein 38-like OS=Cucurbita moschata OX=3662 GN=LOC111454394 PE=4 SV=1)

HSP 1 Score: 329.7 bits (844), Expect = 1.1e-86
Identity = 179/267 (67.04%), Postives = 182/267 (68.16%), Query Frame = 0


Query: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60
           MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG
Sbjct: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60

Query: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120
           YGFV                                                 T   P S
Sbjct: 61  YGFV-------------------------------------------------TFRDPQS 120

Query: 121 VRLVRRRPEVTFLISLPENDRLISVNILL-------DFTGRIQTTASYGGLRPSFPPPPV 180
            R+    P            R  + NI +          GRIQTTASYGGLRPSFPPPPV
Sbjct: 121 ARMACANPNPIIC------GRRANCNIAVFGPPRPPPSGGRIQTTASYGGLRPSFPPPPV 180

Query: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 240
           IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA
Sbjct: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 212

Query: 241 APYVPYYMN-QGLFYSPMHQIITPSTG 260
           APYVPYYMN QGLFYSPMHQIITPSTG
Sbjct: 241 APYVPYYMNQQGLFYSPMHQIITPSTG 212

[Close]

BLAST of Carg20536 vs. ExPASy TrEMBL
Match: A0A6J1KU92 (RNA-binding protein 38-like OS=Cucurbita maxima OX=3661 GN=LOC111496459 PE=4 SV=1)

HSP 1 Score: 324.3 bits (830), Expect = 4.7e-85
Identity = 174/266 (65.41%), Postives = 179/266 (67.29%), Query Frame = 0


Query: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKG 60
           MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDK TGKSKG
Sbjct: 1   MADHFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKITGKSKG 60

Query: 61  YGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSS 120
           YGFV                                                 T   P S
Sbjct: 61  YGFV-------------------------------------------------TFRDPQS 120

Query: 121 VRLVRRRPEVTFLISLPENDRLISVNILL-------DFTGRIQTTASYGGLRPSFPPPPV 180
            R+    P            R  + NI +          GRIQ+TASYGG+RPSFPPPPV
Sbjct: 121 ARMACANPNPIIC------GRRANCNIAVFGPPRPPPSGGRIQSTASYGGIRPSFPPPPV 180

Query: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 240
           IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA
Sbjct: 181 IFPPYRYRSYAPNYTVPYGYGYGIGYGHGYGYGHGYGYGYDCSSSSPAPRAPFSSPSSAA 211

Query: 241 APYVPYYMNQGLFYSPMHQIITPSTG 260
           APYVPYYMNQGLFYS MHQII PSTG
Sbjct: 241 APYVPYYMNQGLFYSQMHQIIPPSTG 211

[Close]

BLAST of Carg20536 vs. ExPASy TrEMBL
Match: A0A6J1HBJ1 (RNA-binding protein 38-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111462586 PE=4 SV=1)

HSP 1 Score: 172.9 bits (437), Expect = 1.7e-39
Identity = 124/299 (41.47%), Postives = 152/299 (50.84%), Query Frame = 0


Query: 1   MADHFSNP-PPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSK 60
           MA++FSN  PP FGDTTWTK+FVGGLAWET+SDEM  FFQQFGEILEAVII+DK TGKSK
Sbjct: 1   MAENFSNTHPPPFGDTTWTKLFVGGLAWETQSDEMRAFFQQFGEILEAVIIRDKNTGKSK 60

Query: 61  GYGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPS 120
           GYGFV+          +    +           I  RR                   + +
Sbjct: 61  GYGFVT----------FRDPDSARRACANPNPIICGRR------------------ANCN 120

Query: 121 SVRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTA----SYGGLRPSFPPPP--- 180
                R RP     + L   +++          G +QTT+    SYGGL   +PPPP   
Sbjct: 121 IAAFGRARPPPPPPLLLGGRNQI----------GNLQTTSAAAGSYGGLLTPYPPPPSPP 180

Query: 181 ---VIFPPYRYRSYAPNYTVPY-------------GYGYGIGYGHG-----------YGY 240
              ++FP YRYRSYAPNYT+PY              YGYG GY              +GY
Sbjct: 181 PPQIMFPAYRYRSYAPNYTLPYHQAQLYPQSPCSSSYGYGYGYPSSSSPSPRGAFPVHGY 240

Query: 241 GHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYY-----MNQGLFYSPMHQIITPSTG 260
           GHG+G+G+D            SSP S++  Y PYY     M Q LFY+ MHQI TP+TG
Sbjct: 241 GHGHGHGHDP-----------SSPLSSSPSYFPYYTNYTHMQQPLFYAHMHQIATPTTG 250

[Close]

BLAST of Carg20536 vs. ExPASy TrEMBL
Match: A0A6J1HBU2 (RNA-binding protein 38-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111462586 PE=4 SV=1)

HSP 1 Score: 169.5 bits (428), Expect = 1.9e-38
Identity = 124/308 (40.26%), Postives = 152/308 (49.35%), Query Frame = 0


Query: 1   MADHFSNP-PPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSK 60
           MA++FSN  PP FGDTTWTK+FVGGLAWET+SDEM  FFQQFGEILEAVII+DK TGKSK
Sbjct: 1   MAENFSNTHPPPFGDTTWTKLFVGGLAWETQSDEMRAFFQQFGEILEAVIIRDKNTGKSK 60

Query: 61  GYGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPS 120
           GYGFV+          +    +           I  RR                   + +
Sbjct: 61  GYGFVT----------FRDPDSARRACANPNPIICGRR------------------ANCN 120

Query: 121 SVRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTA----SYGGLRPSFPPPP--- 180
                R RP     + L   +++          G +QTT+    SYGGL   +PPPP   
Sbjct: 121 IAAFGRARPPPPPPLLLGGRNQI----------GNLQTTSAAAGSYGGLLTPYPPPPSPP 180

Query: 181 ---VIFPPYRYRSYAPNYTVPY----------------------GYGYGIGYGHG----- 240
              ++FP YRYRSYAPNYT+PY                       YGYG GY        
Sbjct: 181 PPQIMFPAYRYRSYAPNYTLPYHQAIYNPQLQSAQLYPQSPCSSSYGYGYGYPSSSSPSP 240

Query: 241 ------YGYGHGYGYGYDCSSSSPAPRAPFSSPSSAAAPYVPYY-----MNQGLFYSPMH 260
                 +GYGHG+G+G+D            SSP S++  Y PYY     M Q LFY+ MH
Sbjct: 241 RGAFPVHGYGHGHGHGHDP-----------SSPLSSSPSYFPYYTNYTHMQQPLFYAHMH 259

[Close]

BLAST of Carg20536 vs. ExPASy TrEMBL
Match: A0A0A0LLP2 (RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368990 PE=4 SV=1)

HSP 1 Score: 167.2 bits (422), Expect = 9.5e-38
Identity = 126/291 (43.30%), Postives = 146/291 (50.17%), Query Frame = 0


Query: 1   MADH----FSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTG 60
           MAD+     ++PPP  GDTTWTK+FVGGLAWET+S EMH FFQQFG+ILEAVII+DK TG
Sbjct: 1   MADNNNFPLNHPPPFAGDTTWTKLFVGGLAWETQSHEMHSFFQQFGDILEAVIIQDKHTG 60

Query: 61  KSKGYGFVSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVT 120
           KSKGYGFV+           D  SA                               +   
Sbjct: 61  KSKGYGFVTF---------KDPESA------------------------------RRACA 120

Query: 121 SPSSVRLVRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTA-----SYGGLRPSFPPP 180
           +P+ +   RR          P    L   +   +  G +Q+TA     SYGGLRP FPPP
Sbjct: 121 NPNPIICGRRANCNIAAFGRPRPPPLSPSSGGRNQIGNLQSTAPAAAGSYGGLRPPFPPP 180

Query: 181 PVIFPPYRYRSYAPNYTVPYG-------------YGYGIGYGHGYGYGHGYGYGYDCS-S 240
           P+IFP YRYRSYAPNYTVPY              Y             + Y YGY  S S
Sbjct: 181 PLIFPHYRYRSYAPNYTVPYHQAIYNPQIQQPQMYQQSPSPSPSSSSSYYYSYGYSSSPS 240

Query: 241 SSPAPRAPFS----SPSSAAAPYVPYYMN------QGLFYSPMHQIITPST 259
           SS  PRA FS     P S +  Y PYY N      QGL Y+PM QIITPST
Sbjct: 241 SSQLPRAAFSVHGHPPQSPS--YFPYYTNYTHMQQQGLVYTPMPQIITPST 250

[Close]

BLAST of Carg20536 vs. TAIR 10
Match: AT1G78260.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 99.4 bits (246), Expect = 4.7e-21
Identity = 45/54 (83.33%), Postives = 50/54 (92.59%), Query Frame = 0


Query: 12 FGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          FGDTT+TKVFVGGLAWET +DEM R+F+QFGEILEAVII DK TGKSKGYGFV+
Sbjct: 11 FGDTTYTKVFVGGLAWETPTDEMRRYFEQFGEILEAVIITDKNTGKSKGYGFVT 64

[Close]

BLAST of Carg20536 vs. TAIR 10
Match: AT1G78260.2 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 99.4 bits (246), Expect = 4.7e-21
Identity = 45/54 (83.33%), Postives = 50/54 (92.59%), Query Frame = 0


Query: 12 FGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          FGDTT+TKVFVGGLAWET +DEM R+F+QFGEILEAVII DK TGKSKGYGFV+
Sbjct: 11 FGDTTYTKVFVGGLAWETPTDEMRRYFEQFGEILEAVIITDKNTGKSKGYGFVT 64

[Close]

BLAST of Carg20536 vs. TAIR 10
Match: AT1G22330.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 97.1 bits (240), Expect = 2.3e-20
Identity = 45/54 (83.33%), Postives = 48/54 (88.89%), Query Frame = 0


Query: 12 FGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGFVS 66
          FGDTT TKVFVGGLAWET +DEM R+F QFGEILEAVII DK TGKSKGYGFV+
Sbjct: 11 FGDTTHTKVFVGGLAWETPTDEMRRYFDQFGEILEAVIITDKATGKSKGYGFVT 64

[Close]

BLAST of Carg20536 vs. TAIR 10
Match: AT1G76460.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 93.6 bits (231), Expect = 2.6e-19
Identity = 42/62 (67.74%), Postives = 53/62 (85.48%), Query Frame = 0


Query: 4  HFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGF 63
          H+ N P  FGDTT+TKVFVGGLAWET+S+ + + F+Q+GEILEAV+I DK TG+SKGYGF
Sbjct: 12 HYLNSP--FGDTTFTKVFVGGLAWETQSETLRQHFEQYGEILEAVVIADKNTGRSKGYGF 71

Query: 64 VS 66
          V+
Sbjct: 72 VT 71

[Close]

BLAST of Carg20536 vs. TAIR 10
Match: AT1G20880.1 (RNA-binding (RRM/RBD/RNP motifs) family protein )

HSP 1 Score: 80.1 bits (196), Expect = 2.9e-15
Identity = 83/250 (33.20%), Postives = 108/250 (43.20%), Query Frame = 0


Query: 4   HFSNPPPLFGDTTWTKVFVGGLAWETKSDEMHRFFQQFGEILEAVIIKDKTTGKSKGYGF 63
           H+ N P  FGDTT+TKVFVGGLAWET+S+ + R F Q+G+ILEAV+I DK TG+SKGYGF
Sbjct: 12  HYLNSP--FGDTTFTKVFVGGLAWETQSETLRRHFDQYGDILEAVVITDKNTGRSKGYGF 71

Query: 64  VSSLFLSDSISGYDFNSAVSDFVIRSLSEILNRREWPALIQIRLSVEDEQTVTSPSSVRL 123
           V+          +    A     +     I  RR    L  +  S    Q    P +   
Sbjct: 72  VT----------FRDPEAARRACVDPTPIIDGRRANCNLASLGRSRPPMQYAVIPHAPGR 131

Query: 124 VRRRPEVTFLISLPENDRLISVNILLDFTGRIQTTASYG-GLRPSFP-----PPPVIFPP 183
           VR        +  P      S       T   Q    Y  G+ P  P          + P
Sbjct: 132 VRPPSPYVGSVQSPRGLHFGSHPYHQPPTYNYQQGVVYPYGVTPYGPDYMYSQSQGFYGP 191

Query: 184 YRYRSYAPNYTVP-----YGYGYG---------IGYGHGYGYGHGYGYGYDCSSSSP--A 232
           Y  + Y   Y VP      GY YG          GY  G GY H   +     ++SP  A
Sbjct: 192 YMGQQYLQVYGVPGAVNSPGYQYGQLSHTIPGAHGYTAGQGYSHPGSHVLQLGATSPMSA 249

The following BLAST results are available for this feature:

BLAST of Carg20536 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020218.1	2.1e-148	100.00	putative RNA-binding protein ARP1, partial [Cucurbita argyrosperma subsp. argyro...	[more]
XP_023538118.1	9.3e-88	67.29	RNA-binding protein 38-like [Cucurbita pepo subsp. pepo]	[more]
XP_022951640.1	2.3e-86	67.04	RNA-binding protein 38-like [Cucurbita moschata]	[more]
XP_023002673.1	9.6e-85	65.41	RNA-binding protein 38-like [Cucurbita maxima]	[more]
KAG6585298.1	1.4e-72	64.34	putative RNA-binding protein ARP1, partial [Cucurbita argyrosperma subsp. sorori...	[more]

BLAST of Carg20536 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M1S3	4.6e-13	64.81	Probable RNA-binding protein ARP1 OS=Arabidopsis thaliana OX=3702 GN=ARP1 PE=2 S...	[more]
Q76LC6	1.5e-11	57.69	RNA-binding protein 24 OS=Danio rerio OX=7955 GN=rbm24 PE=2 SV=1	[more]
Q9BX46	2.5e-11	57.69	RNA-binding protein 24 OS=Homo sapiens OX=9606 GN=RBM24 PE=1 SV=1	[more]
Q6GQD3	3.3e-11	55.77	RNA-binding protein 24-A OS=Xenopus laevis OX=8355 GN=rbm24-a PE=2 SV=1	[more]
Q9I8B3	3.3e-11	55.77	RNA-binding protein 24-B OS=Xenopus laevis OX=8355 GN=rbm24-b PE=2 SV=1	[more]

BLAST of Carg20536 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GJE8	1.1e-86	67.04	RNA-binding protein 38-like OS=Cucurbita moschata OX=3662 GN=LOC111454394 PE=4 S...	[more]
A0A6J1KU92	4.7e-85	65.41	RNA-binding protein 38-like OS=Cucurbita maxima OX=3661 GN=LOC111496459 PE=4 SV=...	[more]
A0A6J1HBJ1	1.7e-39	41.47	RNA-binding protein 38-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A6J1HBU2	1.9e-38	40.26	RNA-binding protein 38-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC11146...	[more]
A0A0A0LLP2	9.5e-38	43.30	RRM domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G368990 PE=4 SV...	[more]

BLAST of Carg20536 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G78260.1	4.7e-21	83.33	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G78260.2	4.7e-21	83.33	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G22330.1	2.3e-20	83.33	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G76460.1	2.6e-19	67.74	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]
AT1G20880.1	2.9e-15	33.20	RNA-binding (RRM/RBD/RNP motifs) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000504	RNA recognition motif domain	SMART	SM00360	rrm1_1	coord: 19..86 e-value: 5.0E-10 score: 49.3
IPR000504	RNA recognition motif domain	PFAM	PF00076	RRM_1	coord: 20..64 e-value: 7.3E-12 score: 45.0
IPR000504	RNA recognition motif domain	PROSITE	PS50102	RRM	coord: 18..65 score: 12.884388
IPR012677	Nucleotide-binding alpha-beta plait domain superfamily	GENE3D	3.30.70.330		coord: 16..65 e-value: 1.2E-17 score: 65.7
None	No IPR available	PANTHER	PTHR11176:SF23	RNA-BINDING (RRM/RBD/RNP MOTIFS) FAMILY PROTEIN	coord: 12..65
IPR037366	BOULE/DAZ family	PANTHER	PTHR11176	BOULE-RELATED	coord: 12..65
IPR035979	RNA-binding domain superfamily	SUPERFAMILY	54928	RNA-binding domain, RBD	coord: 9..64

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20536-RA	Carg20536-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0003676	nucleic acid binding