Carg20518 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20518
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Spermidine synthase
Location	Carg_Chr12: 17245 .. 19850 (+)
RNA-Seq Expression	Carg20518
Synteny	Carg20518

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCGTCCTTTTTTTAGCAGATTCGCTGCTTGTGAACCTGTCAATCTTTTTTTCATAACAAAGCAGCCGACCCCACCCTCTTAGTTCCGGGGCGTCTTGCTCGTGCTCGAAGGGCTAACGACCAAGCGGCTCCGATAATTCTTCTGCCTTGGCCTCAGATTTATCTGTGAAGAGGTCGAGGGACGAAGACCAAAATGGAACTCCACCCTCTTCACAACCTGATTCTATTTCTTCCGTCATCCCCGGATGGTTCTCCGAGATCAGCCCAATGTGGCCTGGTGGGTTCGCCTCTACATCTCGCTCCTCTTACTTAATATGTGTTTATTCCTAAGTAATCGTTCCAAAGTCATCGACAGTTTTGGAACTCATTCACTTGTGCCTTCTCTGCTTTCGGAGTCGTAGTGTTTTCCCTTTATCTGATTTTAAACTCCTCTGGTGTGGTTTACTTCTACGCGGGGATAGGAAGATGCAGTTTATTAGCTATTTAAATGCACGAGCTTATATTTAATCGGTATTCTATGCCCAATTTTATGTTTTCCGCTGGTTTCTGTCACTTGTTTCTCTGTTTAAAATTTGTGTTCTTCTATGGCGTCGTTTCATTTGGTTGCTAACAGTTGCTATTGTAGGCTCAGCTTAAATTATCGATATGTTTAGTCTTAAATAATAGGTTTATTTCCCCTTTGCTCTTTGCGAAGGTCCATTTTGCTTCGCACATTCTCTTCGCAAATGGTATCATTCTTGGTTGTTGGCAAGCTTGGATGGTGGGTTATGCCACTGTATTTGTATTGACTCCGTTTGATGTGAGTTTTCTTTTTGTGTTGGCTGCTGTATATCCTTTAATTTACTCTATTAGAGTATCTTTTTTAATAAGAAAAAAAATGATAAATTTTTGTTACGAAATAAACAGGATAAATATGCTGTGAACTTGTCCATGACGGTATCACTTTTTTTCTTGTCCGTACTTATAACTTGAAAATATTGTGCAGGAGAAGCACATTCATTGAAAGTAGAGAAGATTTTATTTCGAGGGAATTCTGACTATCAGGATGTTATGGTCTTTCAGGTGTGAATTACTTGAGCATTGTAATATTTCTTCTTTGGCTTGTTATTGATTCTATCCCAACTGTTTGCTGACGGGGATGCTTTCTTTTTGTAGTCATCAACTCTTATGGGAAGGTTCTTGTTCTGGATGGAGTCATCCAGCTGACTGAGAGAGATGAATGTGCTTACCAAGAAATGATTGCTCACCTACCACTTTGCTCAATTTCTAATCCGACTAAGGTTTTTAATCTTCTATTAAATGTACAAAAATTTTCGATTGATTCTCTTCTATACACAATCTATTTTAGGCCATATGCATTCTTCAATAATTACTATTGATTATAATTGCATTATTCTGAAAAGGTTTTGGTTATTGGTGGAGGAGATGGTGGAGTGCTTCGTGAAGTTTCTCGCCATTCTTCTGTTAAGCAGATAGATATCTGTGAAATTGATAAGATGGTGGTTGATGGAAGTAATATCCTTGTTTCTCCTTAATATACTGCTACTCCCCCACCTCCCCCCGCATATAAGTTGGTATCTTACCAAAAACTAATTTGAGAATAGTAACGGTTATCTATGTAGTTTTTTCACTCCATAATTTCGCATCTCAGTTGGCCTCTAAATGTGAGGGACAACTTACAATAATGGATGCTACGTGACTGCTTTTGCAGGTTTCAAAACAATTTTTCCCTGATGTAGCTGTTGGGTTTGAGGATCCACGGGTCACACTTCATATTGGCGATGGTAATCAGCTATGCTGCGTTTTTTTTCCCAGTTTTCACTTTTTCCATGGGTTTCTGCTTGGACTTTTCTTGCTTTTAAATGTTTGCAGGAGTTGCATTGTTAAAGGCTGTCCCCGAAGGGACTTATGATGTTATTATAGTGGATTCTTCTGATCCAATAGGTAAATTATCTGCCTGCGCAATCTCAAATTCTTCTATGATTCCTGAAGCTATCTTTTCAACTCTTAAATTTTTTTGATTGCATCTTCAGGTCCTGCGCAAGAGCTTTTTGAGAAGCCATTTTTTGAATCCGCTGCCAGAGCTGTTCGACCAGGAGGTGTTGTGTGCACTCAGGCAGAAAGTATATGGCTTCATATGCACATTATTGAGGACATAGTCACAAACTGTCGCCAAGTATTCAAAGGCTCAGTAAATTATGCATGGACTACAGTTCCGACGTATCCCAGGCGAGCATTTTCAGATGATTATTAACATGTCTATTCTGCCTATAGATAGACGTGAACATATCTGGCTGCAACATTTAGAGAACCTGCCCCATTCACTTGACAATGCTGCGCAGCATTAGAATGGTGGTTTGGTTGAAATTGAAACCCCAGATTCAATCCATGGTCGGGAGGAGTTGAGAACCTTTCATGTGATATATAACTGACATATATTAGTATTGGTAGTTGGAGTTTTTTTTTTTTTTTTCCTTCTTACAACTAATTCCTTGAAATTTTGTGGCCCAGTGGAGTGATTGGTTTTATGCTGTGCTCTACCGAGGGACCTGCTGTTGATTTCAAACACCCGATAAATTCAATCGATGTTAATGATATTAACAAC

mRNA sequence

ATGCATTTATCTGTGAAGAGGTCGAGGGACGAAGACCAAAATGGAACTCCACCCTCTTCACAACCTGATTCTATTTCTTCCGTCATCCCCGGATGGTTCTCCGAGATCAGCCCAATGTGGCCTGGAGAAGCACATTCATTGAAAGTAGAGAAGATTTTATTTCGAGGGAATTCTGACTATCAGGATGTTATGGTCTTTCAGGTTCTTGTTCTGGATGGAGTCATCCAGCTGACTGAGAGAGATGAATGTGCTTACCAAGAAATGATTGCTCACCTACCACTTTGCTCAATTTCTAATCCGACTAAGGTTTTGGTTATTGGTGGAGGAGATGGTGGAGTGCTTCGTGAAGTTTCTCGCCATTCTTCTGTTAAGCAGATAGATATCTGTGAAATTGATAAGATGGTTTCAAAACAATTTTTCCCTGATGTAGCTGTTGGGTTTGAGGATCCACGGGTCACACTTCATATTGGCGATGGAGTTGCATTGTTAAAGGCTGTCCCCGAAGGGACTTATGATGTTATTATAGTGGATTCTTCTGATCCAATAGGTCCTGCGCAAGAGCTTTTTGAGAAGCCATTTTTTGAATCCGCTGCCAGAGCTGTTCGACCAGGAGGTGTTGTGTGCACTCAGGCAGAAAGTATATGGCTTCATATGCACATTATTGAGGACATAGTCACAAACTGTCGCCAAGTATTCAAAGGCTCAGTAAATTATGCATGGACTACAGTTCCGACGTATCCCAGGCGAGCATTTTCAGATGATTATTAACATGTCTATTCTGCCTATAGATAGACGTGAACATATCTGGCTGCAACATTTAGAGAACCTGCCCCATTCACTTGACAATGCTGCGCAGCATTAGAATGGTGGTTTGGTTGAAATTGAAACCCCAGATTCAATCCATGGTCGGGAGGAGTTGAGAACCTTTCATGTGATATATAACTGACATATATTAGTATTGGTAGTTGGAGTTTTTTTTTTTTTTTTCCTTCTTACAACTAATTCCTTGAAATTTTGTGGCCCAGTGGAGTGATTGGTTTTATGCTGTGCTCTACCGAGGGACCTGCTGTTGATTTCAAACACCCGATAAATTCAATCGATGTTAATGATATTAACAAC

Coding sequence (CDS)

ATGCATTTATCTGTGAAGAGGTCGAGGGACGAAGACCAAAATGGAACTCCACCCTCTTCACAACCTGATTCTATTTCTTCCGTCATCCCCGGATGGTTCTCCGAGATCAGCCCAATGTGGCCTGGAGAAGCACATTCATTGAAAGTAGAGAAGATTTTATTTCGAGGGAATTCTGACTATCAGGATGTTATGGTCTTTCAGGTTCTTGTTCTGGATGGAGTCATCCAGCTGACTGAGAGAGATGAATGTGCTTACCAAGAAATGATTGCTCACCTACCACTTTGCTCAATTTCTAATCCGACTAAGGTTTTGGTTATTGGTGGAGGAGATGGTGGAGTGCTTCGTGAAGTTTCTCGCCATTCTTCTGTTAAGCAGATAGATATCTGTGAAATTGATAAGATGGTTTCAAAACAATTTTTCCCTGATGTAGCTGTTGGGTTTGAGGATCCACGGGTCACACTTCATATTGGCGATGGAGTTGCATTGTTAAAGGCTGTCCCCGAAGGGACTTATGATGTTATTATAGTGGATTCTTCTGATCCAATAGGTCCTGCGCAAGAGCTTTTTGAGAAGCCATTTTTTGAATCCGCTGCCAGAGCTGTTCGACCAGGAGGTGTTGTGTGCACTCAGGCAGAAAGTATATGGCTTCATATGCACATTATTGAGGACATAGTCACAAACTGTCGCCAAGTATTCAAAGGCTCAGTAAATTATGCATGGACTACAGTTCCGACGTATCCCAGGCGAGCATTTTCAGATGATTATTAA

Protein sequence

MHLSVKRSRDEDQNGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDYQDVMVFQVLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRHSSVKQIDICEIDKMVSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAWTTVPTYPRRAFSDDY

Homology

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BLAST of Carg20518 vs. NCBI nr
Match: KAG7020072.1 (Spermidine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 525.8 bits (1353), Expect = 2.1e-145
Identity = 255/255 (100.00%), Postives = 255/255 (100.00%), Query Frame = 0


Query: 1   MHLSVKRSRDEDQNGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDY 60
           MHLSVKRSRDEDQNGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDY
Sbjct: 1   MHLSVKRSRDEDQNGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDY 60

Query: 61  QDVMVFQVLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRH 120
           QDVMVFQVLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRH
Sbjct: 61  QDVMVFQVLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRH 120

Query: 121 SSVKQIDICEIDKMVSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSD 180
           SSVKQIDICEIDKMVSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSD
Sbjct: 121 SSVKQIDICEIDKMVSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSD 180

Query: 181 PIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAW 240
           PIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAW
Sbjct: 181 PIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAW 240

Query: 241 TTVPTYPRRAFSDDY 256
           TTVPTYPRRAFSDDY
Sbjct: 241 TTVPTYPRRAFSDDY 255

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BLAST of Carg20518 vs. NCBI nr
Match: KAF3431739.1 (hypothetical protein FNV43_RR26475 [Rhamnella rubrinervis])

HSP 1 Score: 436.0 bits (1120), Expect = 2.2e-118
Identity = 215/263 (81.75%), Postives = 231/263 (87.83%), Query Frame = 0


Query: 3   LSVKRSRDEDQNG---------TPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKIL 62
           L VKR R+E++NG         T  + + DSIS+VIPGWFSEISPMWPGEAHSLKVEKIL
Sbjct: 10  LPVKRPREEEENGVCVSAVAMETGGTKESDSISTVIPGWFSEISPMWPGEAHSLKVEKIL 69

Query: 63  FRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIG 122
           F+G SDYQDVMVFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSI NP KVLVIG
Sbjct: 70  FQGKSDYQDVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIG 129

Query: 123 GGDGGVLREVSRHSSVKQIDICEIDKMV---SKQFFPDVAVGFEDPRVTLHIGDGVALLK 182
           GGDGGVLREV+RHSSV+QIDICEIDKMV   SKQFFP+VA+GFEDPRVTLHIGDGVA LK
Sbjct: 130 GGDGGVLREVARHSSVEQIDICEIDKMVVDISKQFFPEVAIGFEDPRVTLHIGDGVAFLK 189

Query: 183 AVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDI 242
           AVPEGTYD +IVDSSDPIGPAQELFEKPFFES ++A+RPGGVVCTQAESIWLHMHIIEDI
Sbjct: 190 AVPEGTYDAVIVDSSDPIGPAQELFEKPFFESVSKALRPGGVVCTQAESIWLHMHIIEDI 249

Query: 243 VTNCRQVFKGSVNYAWTTVPTYP 248
           V NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 250 VVNCRQIFKGSVNYAWTTVPTYP 272

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BLAST of Carg20518 vs. NCBI nr
Match: KJB31268.1 (hypothetical protein B456_005G183500 [Gossypium raimondii])

HSP 1 Score: 434.5 bits (1116), Expect = 6.4e-118
Identity = 213/264 (80.68%), Postives = 228/264 (86.36%), Query Frame = 0


Query: 3   LSVKRSRDEDQNGTP----------------PSSQPDSISSVIPGWFSEISPMWPGEAHS 62
           L VKR R++++NG                   S + D ISSVIPGWFSEISPMWPGEAHS
Sbjct: 14  LPVKRPREDEENGVSSAAAAVAMEMEADTNNTSKEVDGISSVIPGWFSEISPMWPGEAHS 73

Query: 63  LKVEKILFRGNSDYQDVMVFQVLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVI 122
           LKVEK+LF+G SDYQ+VMVFQVLVLDGVIQLTERDECAYQEMI HLPLCSI NP KVLVI
Sbjct: 74  LKVEKVLFQGKSDYQNVMVFQVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVI 133

Query: 123 GGGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALL 182
           GGGDGGVLREVSRHSSV+QIDICEIDKM   VSKQ+FPDVAVG++DPRV LHIGDGVA L
Sbjct: 134 GGGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQYFPDVAVGYDDPRVKLHIGDGVAFL 193

Query: 183 KAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIED 242
           KAVPEGTYD IIVDSSDPIGPAQELFEKPFFES A+A+RPGGVVCTQAESIWLHMHIIED
Sbjct: 194 KAVPEGTYDAIIVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIED 253

Query: 243 IVTNCRQVFKGSVNYAWTTVPTYP 248
           IV NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 254 IVANCRQIFKGSVNYAWTTVPTYP 277

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BLAST of Carg20518 vs. NCBI nr
Match: XP_038702739.1 (spermidine synthase [Tripterygium wilfordii] >KAF5746606.1 Spermidine synthase 1 [Tripterygium wilfordii])

HSP 1 Score: 434.1 bits (1115), Expect = 8.3e-118
Identity = 215/264 (81.44%), Postives = 229/264 (86.74%), Query Frame = 0


Query: 3   LSVKRSRDEDQNGTPPSS----------QPDSISSVIPGWFSEISPMWPGEAHSLKVEKI 62
           L VKR R++++NG P ++           PDSISSVIPGWFSEISPMWPGEAHSLKVEKI
Sbjct: 72  LPVKRPREDEENGVPVNTLSMETENNEELPDSISSVIPGWFSEISPMWPGEAHSLKVEKI 131

Query: 63  LFRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVI 122
           LFRG SDYQDVMVFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSI +P KVLVI
Sbjct: 132 LFRGKSDYQDVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPSPKKVLVI 191

Query: 123 GGGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALL 182
           GGGDGGVLREVSRH SV+ IDICEIDKM   VSK+FFP +AVG+EDPRVTLHIGDGVA L
Sbjct: 192 GGGDGGVLREVSRHVSVEHIDICEIDKMVVDVSKEFFPQIAVGYEDPRVTLHIGDGVAFL 251

Query: 183 KAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIED 242
           KAVPEGTYD +IVDSSDPIGPAQELFEKPFFES A+A+RPGGVVCTQAESIWLHMHIIED
Sbjct: 252 KAVPEGTYDAVIVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIED 311

Query: 243 IVTNCRQVFKGSVNYAWTTVPTYP 248
           IV NCRQVFKGSVNYAWTTVPTYP
Sbjct: 312 IVANCRQVFKGSVNYAWTTVPTYP 335

[Close]

BLAST of Carg20518 vs. NCBI nr
Match: XP_030961197.1 (spermidine synthase 1 [Quercus lobata])

HSP 1 Score: 433.0 bits (1112), Expect = 1.9e-117
Identity = 216/263 (82.13%), Postives = 228/263 (86.69%), Query Frame = 0


Query: 5   VKRSRDEDQ-----------NGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKIL 64
           VKR R+E++            G+    QPD IS+VIPGWFSEISPMWPGEAHSLKVEKIL
Sbjct: 14  VKRPREEEEENGVSSVSMEVEGSNKEQQPDCISAVIPGWFSEISPMWPGEAHSLKVEKIL 73

Query: 65  FRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIG 124
           F+GNS+YQ VMVFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSISNP KVLVIG
Sbjct: 74  FQGNSEYQKVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMIVHLPLCSISNPKKVLVIG 133

Query: 125 GGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLK 184
           GGDGGVLREVSRHSSV+QIDICEIDKM   VSKQFFP VAVG+EDPRVTLHIGDGVA LK
Sbjct: 134 GGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQFFPAVAVGYEDPRVTLHIGDGVAFLK 193

Query: 185 AVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDI 244
           AVPEGTYD +IVDSSDPIGPAQELFEKPFFES ARA+RPGGVVCTQAESIWLHMHIIEDI
Sbjct: 194 AVPEGTYDAVIVDSSDPIGPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDI 253

Query: 245 VTNCRQVFKGSVNYAWTTVPTYP 248
           V NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 254 VKNCRQIFKGSVNYAWTTVPTYP 276

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BLAST of Carg20518 vs. ExPASy Swiss-Prot
Match: Q9ZTR1 (Spermidine synthase 1 OS=Pisum sativum OX=3888 GN=SPDSYN1 PE=2 SV=1)

HSP 1 Score: 426.0 bits (1094), Expect = 3.0e-118
Identity = 208/256 (81.25%), Postives = 226/256 (88.28%), Query Frame = 0


Query: 5   VKRSRDEDQNGTPPS----SQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDY 64
           +KR R+++ NG   +     QP+ +SSVIPGWFSEISPMWPGEAHSLKVEKILF+G SDY
Sbjct: 15  LKRQREDEVNGVSDTLSKEPQPNGLSSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDY 74

Query: 65  QDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVL 124
           QDVMVFQ      VL+LDGVIQLTERDECAYQEMI HLPLCSI NP KVLVIGGGDGGVL
Sbjct: 75  QDVMVFQSATYGKVLILDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVL 134

Query: 125 REVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTY 184
           REV+RHSSV++IDICEIDKM   VSK++FPD+AVGF DPRVTL+IGDGVA LKA PEGTY
Sbjct: 135 REVARHSSVEKIDICEIDKMVVDVSKEYFPDIAVGFADPRVTLNIGDGVAFLKAAPEGTY 194

Query: 185 DVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQV 244
           D +IVDSSDPIGPAQELFEKPFFES ARA+RPGGVVCTQAESIWLHMHIIEDIV NCRQV
Sbjct: 195 DAVIVDSSDPIGPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDIVVNCRQV 254

Query: 245 FKGSVNYAWTTVPTYP 248
           FKGSVNYAWTTVPTYP
Sbjct: 255 FKGSVNYAWTTVPTYP 270

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BLAST of Carg20518 vs. ExPASy Swiss-Prot
Match: O82147 (Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1)

HSP 1 Score: 425.6 bits (1093), Expect = 3.9e-118
Identity = 207/235 (88.09%), Postives = 216/235 (91.91%), Query Frame = 0


Query: 22  PDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDYQDVMVFQ------VLVLDGVI 81
           PD +SSVIPGWFSEISPMWPGEAHSLKVEKILF+G SDYQ+VMVFQ      VLVLDGVI
Sbjct: 17  PDGVSSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLVLDGVI 76

Query: 82  QLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRHSSVKQIDICEIDKM- 141
           QLTERDECAYQEMIAHLPLCSI +P KVLVIGGGDGGVLREV+RH SV+QIDICEIDKM 
Sbjct: 77  QLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARHLSVEQIDICEIDKMV 136

Query: 142 --VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSDPIGPAQELFEKP 201
             VSKQFFPDVAVGFEDPRV LHIGDGVA LKAVPEGTYD IIVDSSDPIGPAQELFEKP
Sbjct: 137 VDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKP 196

Query: 202 FFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAWTTVPTYP 248
           FFES A+A+RPGGVVCTQAESIWLHMHIIEDIV NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 197 FFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYP 251

[Close]

BLAST of Carg20518 vs. ExPASy Swiss-Prot
Match: Q9SMB1 (Spermidine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPDSYN1 PE=2 SV=1)

HSP 1 Score: 416.8 bits (1070), Expect = 1.8e-115
Identity = 203/253 (80.24%), Postives = 220/253 (86.96%), Query Frame = 0


Query: 4   SVKRSRDEDQNGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDYQDV 63
           + KR+R+        + +   IS+VIPGWFSEISPMWPGEAHSLKVEK+LF+G SDYQ+V
Sbjct: 6   AAKRARESGDAAAAGAGEQAGISAVIPGWFSEISPMWPGEAHSLKVEKVLFQGKSDYQNV 65

Query: 64  MVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREV 123
           MVFQ      VLVLDGVIQ+TERDECAYQEMI HLPLCSI +P KVLVIGGGDGGVLREV
Sbjct: 66  MVFQSSTYGKVLVLDGVIQVTERDECAYQEMITHLPLCSIKDPKKVLVIGGGDGGVLREV 125

Query: 124 SRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVI 183
           SRHSSV+QIDICEIDKM   VSKQFFP +AVGFEDPRV+LHIGDGVA LK  PEGTYD +
Sbjct: 126 SRHSSVEQIDICEIDKMVVDVSKQFFPHLAVGFEDPRVSLHIGDGVAFLKNAPEGTYDAV 185

Query: 184 IVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKG 243
           IVDSSDPIGPAQELFEKPFF+S ARA+RPGGVVCTQAESIWLHMHIIEDIV NCRQVFKG
Sbjct: 186 IVDSSDPIGPAQELFEKPFFQSVARALRPGGVVCTQAESIWLHMHIIEDIVANCRQVFKG 245

Query: 244 SVNYAWTTVPTYP 248
           SVNYAWTTVPTYP
Sbjct: 246 SVNYAWTTVPTYP 258

[Close]

BLAST of Carg20518 vs. ExPASy Swiss-Prot
Match: Q96556 (Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1)

HSP 1 Score: 414.1 bits (1063), Expect = 1.2e-114
Identity = 198/232 (85.34%), Postives = 212/232 (91.38%), Query Frame = 0


Query: 25  ISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDYQDVMVFQ------VLVLDGVIQLT 84
           ISS++PGWFSEISP+WPGEAHSLKVEKILF+G SDYQ+VMVFQ      VL+LDGVIQLT
Sbjct: 12  ISSILPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLILDGVIQLT 71

Query: 85  ERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRHSSVKQIDICEIDKM---V 144
           ERDECAYQEMI HLPLCSI NP KVLVIGGGDGGVLREVSRHSSV+QIDICEIDKM   V
Sbjct: 72  ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDV 131

Query: 145 SKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSDPIGPAQELFEKPFFE 204
           SKQFFP+VA+G+EDPRV LH+GDGVA LK V EGTYD +IVDSSDPIGPAQELFEKPFFE
Sbjct: 132 SKQFFPNVAIGYEDPRVKLHVGDGVAFLKFVAEGTYDAVIVDSSDPIGPAQELFEKPFFE 191

Query: 205 SAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAWTTVPTYP 248
           S ARA+RPGGVVCTQAESIWLHMHIIEDIV NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 192 SVARALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYP 243

[Close]

BLAST of Carg20518 vs. ExPASy Swiss-Prot
Match: O48659 (Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1)

HSP 1 Score: 413.3 bits (1061), Expect = 2.0e-114
Identity = 198/232 (85.34%), Postives = 213/232 (91.81%), Query Frame = 0


Query: 25  ISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDYQDVMVFQ------VLVLDGVIQLT 84
           ISS++PGWFSEISP+WPGEAHSLKVEKILF+G SDYQ+V+VFQ      VLVLDGVIQLT
Sbjct: 12  ISSILPGWFSEISPLWPGEAHSLKVEKILFQGKSDYQNVVVFQSSTYGKVLVLDGVIQLT 71

Query: 85  ERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRHSSVKQIDICEIDKM---V 144
           ERDECAYQEMI HLPLCSI NP KVLVIGGGDGGVLREVSRHSSV+QIDICEIDKM   V
Sbjct: 72  ERDECAYQEMITHLPLCSIPNPKKVLVIGGGDGGVLREVSRHSSVEQIDICEIDKMVIDV 131

Query: 145 SKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSDPIGPAQELFEKPFFE 204
           SKQFFP+VA+G+EDPRV LH+GDGVA LK VPEGTYD +IVDSSDPIGPAQELFEKPFFE
Sbjct: 132 SKQFFPNVAIGYEDPRVKLHVGDGVAFLKNVPEGTYDAVIVDSSDPIGPAQELFEKPFFE 191

Query: 205 SAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAWTTVPTYP 248
           S ARA+ PGGVVCTQAESIWLHMHIIEDIV+NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 192 SVARALCPGGVVCTQAESIWLHMHIIEDIVSNCRQIFKGSVNYAWTTVPTYP 243

[Close]

BLAST of Carg20518 vs. ExPASy TrEMBL
Match: A0A0D2PV29 (PABS domain-containing protein OS=Gossypium raimondii OX=29730 GN=B456_005G183500 PE=3 SV=1)

HSP 1 Score: 434.5 bits (1116), Expect = 3.1e-118
Identity = 213/264 (80.68%), Postives = 228/264 (86.36%), Query Frame = 0


Query: 3   LSVKRSRDEDQNGTP----------------PSSQPDSISSVIPGWFSEISPMWPGEAHS 62
           L VKR R++++NG                   S + D ISSVIPGWFSEISPMWPGEAHS
Sbjct: 14  LPVKRPREDEENGVSSAAAAVAMEMEADTNNTSKEVDGISSVIPGWFSEISPMWPGEAHS 73

Query: 63  LKVEKILFRGNSDYQDVMVFQVLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVI 122
           LKVEK+LF+G SDYQ+VMVFQVLVLDGVIQLTERDECAYQEMI HLPLCSI NP KVLVI
Sbjct: 74  LKVEKVLFQGKSDYQNVMVFQVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVI 133

Query: 123 GGGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALL 182
           GGGDGGVLREVSRHSSV+QIDICEIDKM   VSKQ+FPDVAVG++DPRV LHIGDGVA L
Sbjct: 134 GGGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQYFPDVAVGYDDPRVKLHIGDGVAFL 193

Query: 183 KAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIED 242
           KAVPEGTYD IIVDSSDPIGPAQELFEKPFFES A+A+RPGGVVCTQAESIWLHMHIIED
Sbjct: 194 KAVPEGTYDAIIVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIED 253

Query: 243 IVTNCRQVFKGSVNYAWTTVPTYP 248
           IV NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 254 IVANCRQIFKGSVNYAWTTVPTYP 277

[Close]

BLAST of Carg20518 vs. ExPASy TrEMBL
Match: A0A7J7DKK7 (Spermidine synthase 1 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW06G00777 PE=3 SV=1)

HSP 1 Score: 434.1 bits (1115), Expect = 4.0e-118
Identity = 215/264 (81.44%), Postives = 229/264 (86.74%), Query Frame = 0


Query: 3   LSVKRSRDEDQNGTPPSS----------QPDSISSVIPGWFSEISPMWPGEAHSLKVEKI 62
           L VKR R++++NG P ++           PDSISSVIPGWFSEISPMWPGEAHSLKVEKI
Sbjct: 72  LPVKRPREDEENGVPVNTLSMETENNEELPDSISSVIPGWFSEISPMWPGEAHSLKVEKI 131

Query: 63  LFRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVI 122
           LFRG SDYQDVMVFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSI +P KVLVI
Sbjct: 132 LFRGKSDYQDVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPSPKKVLVI 191

Query: 123 GGGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALL 182
           GGGDGGVLREVSRH SV+ IDICEIDKM   VSK+FFP +AVG+EDPRVTLHIGDGVA L
Sbjct: 192 GGGDGGVLREVSRHVSVEHIDICEIDKMVVDVSKEFFPQIAVGYEDPRVTLHIGDGVAFL 251

Query: 183 KAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIED 242
           KAVPEGTYD +IVDSSDPIGPAQELFEKPFFES A+A+RPGGVVCTQAESIWLHMHIIED
Sbjct: 252 KAVPEGTYDAVIVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHIIED 311

Query: 243 IVTNCRQVFKGSVNYAWTTVPTYP 248
           IV NCRQVFKGSVNYAWTTVPTYP
Sbjct: 312 IVANCRQVFKGSVNYAWTTVPTYP 335

[Close]

BLAST of Carg20518 vs. ExPASy TrEMBL
Match: A0A7N2L8U6 (PABS domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1)

HSP 1 Score: 433.0 bits (1112), Expect = 9.0e-118
Identity = 216/263 (82.13%), Postives = 228/263 (86.69%), Query Frame = 0


Query: 5   VKRSRDEDQ-----------NGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKIL 64
           VKR R+E++            G+    QPD IS+VIPGWFSEISPMWPGEAHSLKVEKIL
Sbjct: 14  VKRPREEEEENGVSSVSMEVEGSNKEQQPDCISAVIPGWFSEISPMWPGEAHSLKVEKIL 73

Query: 65  FRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIG 124
           F+GNS+YQ VMVFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSISNP KVLVIG
Sbjct: 74  FQGNSEYQKVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMIVHLPLCSISNPKKVLVIG 133

Query: 125 GGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLK 184
           GGDGGVLREVSRHSSV+QIDICEIDKM   VSKQFFP VAVG+EDPRVTLHIGDGVA LK
Sbjct: 134 GGDGGVLREVSRHSSVEQIDICEIDKMVVDVSKQFFPAVAVGYEDPRVTLHIGDGVAFLK 193

Query: 185 AVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDI 244
           AVPEGTYD +IVDSSDPIGPAQELFEKPFFES ARA+RPGGVVCTQAESIWLHMHIIEDI
Sbjct: 194 AVPEGTYDAVIVDSSDPIGPAQELFEKPFFESVARALRPGGVVCTQAESIWLHMHIIEDI 253

Query: 245 VTNCRQVFKGSVNYAWTTVPTYP 248
           V NCRQ+FKGSVNYAWTTVPTYP
Sbjct: 254 VKNCRQIFKGSVNYAWTTVPTYP 276

[Close]

BLAST of Carg20518 vs. ExPASy TrEMBL
Match: A0A2N9GP81 (PABS domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS29324 PE=3 SV=1)

HSP 1 Score: 431.8 bits (1109), Expect = 2.0e-117
Identity = 215/266 (80.83%), Postives = 232/266 (87.22%), Query Frame = 0


Query: 3   LSVKRSR-DEDQNGTPPSS-----------QPDSISSVIPGWFSEISPMWPGEAHSLKVE 62
           L VKR R +E++NG   +S           QPD IS+VIPGWFSEISP+WPGEAHSLKVE
Sbjct: 12  LPVKRPREEEEENGVSSASTVSMELEGNKEQPDCISAVIPGWFSEISPLWPGEAHSLKVE 71

Query: 63  KILFRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVL 122
           KILF+G S+YQ+VMVFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSISNP KVL
Sbjct: 72  KILFQGKSEYQNVMVFQSSTYGKVLVLDGVIQLTERDECAYQEMIVHLPLCSISNPKKVL 131

Query: 123 VIGGGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVA 182
           VIGGGDGGVLREVSRHSS++QIDICEIDKM   VSKQFFP +AVGFEDPRVTLHIGDGVA
Sbjct: 132 VIGGGDGGVLREVSRHSSIEQIDICEIDKMVVDVSKQFFPKMAVGFEDPRVTLHIGDGVA 191

Query: 183 LLKAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHII 242
            LKAVPEGTYD ++VDSSDPIGPAQELFEKPFFES A+A+RPGGVVCTQAESIWLHMHII
Sbjct: 192 FLKAVPEGTYDAVLVDSSDPIGPAQELFEKPFFESVAKALRPGGVVCTQAESIWLHMHII 251

Query: 243 EDIVTNCRQVFKGSVNYAWTTVPTYP 248
           EDIVTNCRQ+FKGSVNYAWTTVPTYP
Sbjct: 252 EDIVTNCRQIFKGSVNYAWTTVPTYP 277

[Close]

BLAST of Carg20518 vs. ExPASy TrEMBL
Match: A0A068TVD8 (PABS domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T00032046001 PE=3 SV=1)

HSP 1 Score: 431.4 bits (1108), Expect = 2.6e-117
Identity = 209/239 (87.45%), Postives = 219/239 (91.63%), Query Frame = 0


Query: 22  PDSISSVIPGWFSEISPMWPGEAHSLKVEKILFRGNSDYQDVMVFQ------VLVLDGVI 81
           PD +SSVIPGWFSEISPMWPGEAHSLKVEKILF+G SDYQ+VMVFQ      VLVLDGVI
Sbjct: 17  PDGVSSVIPGWFSEISPMWPGEAHSLKVEKILFQGKSDYQNVMVFQSSTYGKVLVLDGVI 76

Query: 82  QLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLREVSRHSSVKQIDICEIDKM- 141
           QLTERDECAYQEMIAHLPLCSI +P KVLVIGGGDGGVLREV+RHSSV+QID+CEIDKM 
Sbjct: 77  QLTERDECAYQEMIAHLPLCSIPSPKKVLVIGGGDGGVLREVARHSSVEQIDLCEIDKMV 136

Query: 142 --VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDVIIVDSSDPIGPAQELFEKP 201
             VSKQFFPDVAVGFEDPRV LHIGDGVA LKAVPEGTYD IIVDSSDPIGPAQELFEKP
Sbjct: 137 VDVSKQFFPDVAVGFEDPRVVLHIGDGVAFLKAVPEGTYDAIIVDSSDPIGPAQELFEKP 196

Query: 202 FFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFKGSVNYAWTTVPTYPRRAF 252
           FFES A+A+RPGGVVCTQAESIWLHMHIIEDIV NCRQ+FKGSVNYAWTTVPTYPR  F
Sbjct: 197 FFESVAKALRPGGVVCTQAESIWLHMHIIEDIVANCRQIFKGSVNYAWTTVPTYPRHCF 255

[Close]

BLAST of Carg20518 vs. TAIR 10
Match: AT1G70310.1 (spermidine synthase 2 )

HSP 1 Score: 404.4 bits (1038), Expect = 6.6e-113
Identity = 201/264 (76.14%), Postives = 221/264 (83.71%), Query Frame = 0


Query: 3   LSVKRSRD----------EDQNGTPPSSQPDSISSVIPGWFSEISPMWPGEAHSLKVEKI 62
           L VKR R+          E +NG     +P  +SS+IPGWFSEISPMWPGEAHSLKVEKI
Sbjct: 12  LPVKRPREAEEDNNGGAMETENGGGEIKEPSCMSSIIPGWFSEISPMWPGEAHSLKVEKI 71

Query: 63  LFRGNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVI 122
           LF+G SDYQDV+VFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSISNP KVLVI
Sbjct: 72  LFQGKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSISNPKKVLVI 131

Query: 123 GGGDGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALL 182
           GGGDGGVLREV+RHSSV+QIDICEIDKM   V+KQ+FP+VAVG+EDPRV L IGDGVA L
Sbjct: 132 GGGDGGVLREVARHSSVEQIDICEIDKMVVDVAKQYFPNVAVGYEDPRVNLIIGDGVAFL 191

Query: 183 KAVPEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIED 242
           K   EGTYD +IVDSSDPIGPA+ELFEKPFFES  RA+RPGGVVCTQAES+WLHM IIED
Sbjct: 192 KNAAEGTYDAVIVDSSDPIGPAKELFEKPFFESVNRALRPGGVVCTQAESLWLHMDIIED 251

Query: 243 IVTNCRQVFKGSVNYAWTTVPTYP 248
           IV+NCR +FKGSVNYAWT+VPTYP
Sbjct: 252 IVSNCRDIFKGSVNYAWTSVPTYP 275

[Close]

BLAST of Carg20518 vs. TAIR 10
Match: AT1G23820.2 (spermidine synthase 1 )

HSP 1 Score: 404.1 bits (1037), Expect = 8.6e-113
Identity = 196/262 (74.81%), Postives = 222/262 (84.73%), Query Frame = 0


Query: 5   VKRSRDEDQNGTPPS---------SQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFR 64
           +KR R+ED NG   +          +P   S+VIPGWFSE+SPMWPGEAHSLKVEK+LF+
Sbjct: 55  LKRPREEDDNGGAATMETENGDQKKEPACFSTVIPGWFSEMSPMWPGEAHSLKVEKVLFQ 114

Query: 65  GNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGG 124
           G SDYQDV+VFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSI NP KVLVIGGG
Sbjct: 115 GKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGG 174

Query: 125 DGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAV 184
           DGGVLREV+RH+S++QID+CEIDKM   VSKQFFPDVA+G+EDPRV L IGDGVA LK  
Sbjct: 175 DGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNA 234

Query: 185 PEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVT 244
            EG+YD +IVDSSDPIGPA+ELFEKPFF+S ARA+RPGGVVCTQAES+WLHM IIEDIV+
Sbjct: 235 AEGSYDAVIVDSSDPIGPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIEDIVS 294

Query: 245 NCRQVFKGSVNYAWTTVPTYPR 249
           NCR++FKGSVNYAWT+VPTYPR
Sbjct: 295 NCREIFKGSVNYAWTSVPTYPR 316

[Close]

BLAST of Carg20518 vs. TAIR 10
Match: AT1G23820.1 (spermidine synthase 1 )

HSP 1 Score: 401.7 bits (1031), Expect = 4.3e-112
Identity = 195/261 (74.71%), Postives = 221/261 (84.67%), Query Frame = 0


Query: 5   VKRSRDEDQNGTPPS---------SQPDSISSVIPGWFSEISPMWPGEAHSLKVEKILFR 64
           +KR R+ED NG   +          +P   S+VIPGWFSE+SPMWPGEAHSLKVEK+LF+
Sbjct: 55  LKRPREEDDNGGAATMETENGDQKKEPACFSTVIPGWFSEMSPMWPGEAHSLKVEKVLFQ 114

Query: 65  GNSDYQDVMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGG 124
           G SDYQDV+VFQ      VLVLDGVIQLTERDECAYQEMI HLPLCSI NP KVLVIGGG
Sbjct: 115 GKSDYQDVIVFQSATYGKVLVLDGVIQLTERDECAYQEMITHLPLCSIPNPKKVLVIGGG 174

Query: 125 DGGVLREVSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAV 184
           DGGVLREV+RH+S++QID+CEIDKM   VSKQFFPDVA+G+EDPRV L IGDGVA LK  
Sbjct: 175 DGGVLREVARHASIEQIDMCEIDKMVVDVSKQFFPDVAIGYEDPRVNLVIGDGVAFLKNA 234

Query: 185 PEGTYDVIIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVT 244
            EG+YD +IVDSSDPIGPA+ELFEKPFF+S ARA+RPGGVVCTQAES+WLHM IIEDIV+
Sbjct: 235 AEGSYDAVIVDSSDPIGPAKELFEKPFFQSVARALRPGGVVCTQAESLWLHMDIIEDIVS 294

Query: 245 NCRQVFKGSVNYAWTTVPTYP 248
           NCR++FKGSVNYAWT+VPTYP
Sbjct: 295 NCREIFKGSVNYAWTSVPTYP 315

[Close]

BLAST of Carg20518 vs. TAIR 10
Match: AT5G53120.1 (spermidine synthase 3 )

HSP 1 Score: 336.7 bits (862), Expect = 1.7e-92
Identity = 163/254 (64.17%), Postives = 197/254 (77.56%), Query Frame = 0


Query: 18  PSSQPDSISSVIPGWFSE---------------ISPMWPGEAHSLKVEKILFRGNSDYQD 77
           P       S+V+ GWFSE                +PMWPGEAHSLKVEK+LF+  SD+Q+
Sbjct: 41  PEDDAKCHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQE 100

Query: 78  VMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLRE 137
           V+VF+      VLVLDG++QLTE+DECAYQEMIAHLPLCSIS+P  VLV+GGGDGGVLRE
Sbjct: 101 VLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLRE 160

Query: 138 VSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDV 197
           +SRHSSV+ IDICEIDKM   VSK+FFP++AVGF+DPRV LHIGD    L+  PEG YD 
Sbjct: 161 ISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDA 220

Query: 198 IIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFK 248
           IIVDSSDP+GPA  L EKPFFE+ ARA++PGGV+C  AES+WLH H+IED+++ CRQ FK
Sbjct: 221 IIVDSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK 280

[Close]

BLAST of Carg20518 vs. TAIR 10
Match: AT5G53120.2 (spermidine synthase 3 )

HSP 1 Score: 336.7 bits (862), Expect = 1.7e-92
Identity = 163/254 (64.17%), Postives = 197/254 (77.56%), Query Frame = 0


Query: 18  PSSQPDSISSVIPGWFSE---------------ISPMWPGEAHSLKVEKILFRGNSDYQD 77
           P       S+V+ GWFSE                +PMWPGEAHSLKVEK+LF+  SD+Q+
Sbjct: 41  PEDDAKCHSTVVSGWFSEPHPRSGKKGGKAVYFNNPMWPGEAHSLKVEKVLFKDKSDFQE 100

Query: 78  VMVFQ------VLVLDGVIQLTERDECAYQEMIAHLPLCSISNPTKVLVIGGGDGGVLRE 137
           V+VF+      VLVLDG++QLTE+DECAYQEMIAHLPLCSIS+P  VLV+GGGDGGVLRE
Sbjct: 101 VLVFESATYGKVLVLDGIVQLTEKDECAYQEMIAHLPLCSISSPKNVLVVGGGDGGVLRE 160

Query: 138 VSRHSSVKQIDICEIDKM---VSKQFFPDVAVGFEDPRVTLHIGDGVALLKAVPEGTYDV 197
           +SRHSSV+ IDICEIDKM   VSK+FFP++AVGF+DPRV LHIGD    L+  PEG YD 
Sbjct: 161 ISRHSSVEVIDICEIDKMVIDVSKKFFPELAVGFDDPRVQLHIGDAAEFLRKSPEGKYDA 220

Query: 198 IIVDSSDPIGPAQELFEKPFFESAARAVRPGGVVCTQAESIWLHMHIIEDIVTNCRQVFK 248
           IIVDSSDP+GPA  L EKPFFE+ ARA++PGGV+C  AES+WLH H+IED+++ CRQ FK
Sbjct: 221 IIVDSSDPVGPALALVEKPFFETLARALKPGGVLCNMAESMWLHTHLIEDMISICRQTFK 280

The following BLAST results are available for this feature:

BLAST of Carg20518 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7020072.1	2.1e-145	100.00	Spermidine synthase 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAF3431739.1	2.2e-118	81.75	hypothetical protein FNV43_RR26475 [Rhamnella rubrinervis]	[more]
KJB31268.1	6.4e-118	80.68	hypothetical protein B456_005G183500 [Gossypium raimondii]	[more]
XP_038702739.1	8.3e-118	81.44	spermidine synthase [Tripterygium wilfordii] >KAF5746606.1 Spermidine synthase 1...	[more]
XP_030961197.1	1.9e-117	82.13	spermidine synthase 1 [Quercus lobata]	[more]

BLAST of Carg20518 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9ZTR1	3.0e-118	81.25	Spermidine synthase 1 OS=Pisum sativum OX=3888 GN=SPDSYN1 PE=2 SV=1	[more]
O82147	3.9e-118	88.09	Spermidine synthase OS=Coffea arabica OX=13443 PE=2 SV=1	[more]
Q9SMB1	1.8e-115	80.24	Spermidine synthase 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SPDSYN1 PE=2 S...	[more]
Q96556	1.2e-114	85.34	Spermidine synthase 1 OS=Datura stramonium OX=4076 PE=2 SV=1	[more]
O48659	2.0e-114	85.34	Spermidine synthase 2 OS=Hyoscyamus niger OX=4079 PE=2 SV=1	[more]

BLAST of Carg20518 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0D2PV29	3.1e-118	80.68	PABS domain-containing protein OS=Gossypium raimondii OX=29730 GN=B456_005G18350...	[more]
A0A7J7DKK7	4.0e-118	81.44	Spermidine synthase 1 OS=Tripterygium wilfordii OX=458696 GN=HS088_TW06G00777 PE...	[more]
A0A7N2L8U6	9.0e-118	82.13	PABS domain-containing protein OS=Quercus lobata OX=97700 PE=3 SV=1	[more]
A0A2N9GP81	2.0e-117	80.83	PABS domain-containing protein OS=Fagus sylvatica OX=28930 GN=FSB_LOCUS29324 PE=...	[more]
A0A068TVD8	2.6e-117	87.45	PABS domain-containing protein OS=Coffea canephora OX=49390 GN=GSCOC_T0003204600...	[more]

BLAST of Carg20518 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G70310.1	6.6e-113	76.14	spermidine synthase 2	[more]
AT1G23820.2	8.6e-113	74.81	spermidine synthase 1	[more]
AT1G23820.1	4.3e-112	74.71	spermidine synthase 1	[more]
AT5G53120.1	1.7e-92	64.17	spermidine synthase 3	[more]
AT5G53120.2	1.7e-92	64.17	spermidine synthase 3	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25

Position :

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IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001045	Spermidine/spermine synthases	TIGRFAM	TIGR00417	TIGR00417	coord: 32..248 e-value: 6.4E-75 score: 249.8
IPR001045	Spermidine/spermine synthases	PANTHER	PTHR11558	SPERMIDINE/SPERMINE SYNTHASE	coord: 5..248
IPR001045	Spermidine/spermine synthases	HAMAP	MF_00198	Spermidine_synth	coord: 31..253 score: 22.528893
IPR035246	Spermidine synthase, tetramerisation domain	PFAM	PF17284	Spermine_synt_N	coord: 31..79 e-value: 2.2E-15 score: 56.2
IPR037163	Spermidine synthase, tetramerisation domain superfamily	GENE3D	2.30.140.10	Spermidine synthase, tetramerisation domain	coord: 24..76 e-value: 2.8E-22 score: 80.9
None	No IPR available	PFAM	PF01564	Spermine_synth	coord: 82..248 e-value: 6.9E-64 score: 214.7
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 77..253 e-value: 7.8E-71 score: 240.2
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..26
None	No IPR available	PANTHER	PTHR11558:SF51	SPERMIDINE/SPERMINE SYNTHASE-RELATED	coord: 5..248
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 102..210 e-value: 2.46236E-11 score: 57.0546
IPR030373	Polyamine biosynthesis domain, conserved site	PROSITE	PS01330	PABS_1	coord: 103..116
IPR030374	Polyamine biosynthesis domain	PROSITE	PS51006	PABS_2	coord: 30..255 score: 77.938606
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 28..247

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20518-RA	Carg20518-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006596	polyamine biosynthetic process
molecular_function	GO:0004766	spermidine synthase activity
molecular_function	GO:0003824	catalytic activity