Carg20358 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20358
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptioncation/H(+) antiporter 4-like
LocationCarg_Chr18: 11697252 .. 11699834 (-)
RNA-Seq ExpressionCarg20358
SyntenyCarg20358
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGTCGCTGATTAAGCATAACTCCACGGAAAATGGGACGAACCAAATTTGTTTGAGTGTGTTGCCTTTCGTAAACTCAGATGGTTTGTGGGCAAAGGCTCATACCTATCAATGGTGGCTCAACAGTTCCTTGCCTCTCTTGGAATTACAGTTGGTTGTGTTCGGTTTGATGATGATAACTATTCAGCTGCTTCTCAAGCGTCTTGGGGCCTCCAAAATTTCCTCTCAAATTTTGGTAACTCTACACATTCCACTTTGTTCTATATTGGGTTAATTGTTAAATACATGAATACGACCAATTTGAGCGTAGCTTTCACAGTTAAAACATCGATAACAAATGGAACCCTTTTAATGATTTGATGATGTTTGCAATTGAAACAGACTGGAATGATATTTGGTTGTTCCTGGGGCACGTTTGACGAAGCAAAATTCAAGCTTTTTAGGATTGACAGTGAAGAGGTGTTGGGATTGCTATCATATTTCGGGTACACGCTATATTTGTTCATAACAGCAGTCAAAATGGATATCAGAATGACTATGCAAACAGGCAAGAAAGCATGGATAATTGGAACGCCTTCAGTTCTTGTGCCTCTATTTTGTGGTCTTTTTGTGTGTTCTTTTCTCCACAAAGGCCTAACTGTTGAAGAGATAAGAAAGCTCCCATTAATGACTTCACTGCAAAGTATGATCTCATTTCCAGTGATTGCTTCAGTTTTAAATGAGATGAAAATCGTGAGCACAGAACTTGGTCGTTTGGGGCTGTCCTCAGCATTAGTTGCAGACATATTCAGCCTAATTTCTATGCTAATTACAAAACAACTGACACAAGAGAGTGCTGCAGAAGTGTATTATGCATTTGGAGCATTGTTTGTTCAAGTCTTTTTGGTGTCATTTTTGTTTAGGCCTGCAGTGGTTTGGATTCTGAAACGAACACCTGAAGGGAAGCCAGTGAGCCGTGGTAGTACCCAAGGAGTTTTTGTTTTGGTTCTGCTTTCTTCTATGACATCTACTGTTTTGGGGCACTCAGTAATCTTAGGGCCTTATTTGTTGGGATTGGCCATTCCTGATGGAGGCCCTGTGGGATATTCTTTTGTTGAGAAGATGGAATCCTTTGTTTCAGATTTTTTCGTGCCACTGTTTGCCATTACTTGTGCTCTCAAGGTGGATTTGTCCAAGCTTTTAATAGCATATGGGGGTGCTTTTACAAGAGTTACCGTAATTCTAGCCTTTGTAACTTGTGTAGTCAAATTCTCGTGTGTCTTTCTTGGCTCATTGTATTGTCAGCTGAGCACTAGAGATTCCTTGGTGCTGTCCCTCATTCTCAGCTGCAAGGGTGTTGTTGAGTTGGCCTTTACCTCACTCTACACTGAATACAGAGTATTTTCTCTGTTTCTCTCTACTTTTGTTGTTAATTGTGTAAGAATCTCGTCTTATTTCTTGTGGGGTTTGGCTGATTTTGCAGGTTATCAGCAAAGGGACGATGGGGTGGATCACATTTTTCATATTCATTCTTGCAACCTTTGCGCCAATTGGAGTGAAATGCGTGAATGACCTTTCAAGGAAACAAGCAAGCAATCAGAACAGGAGCATCATGCACATGAGCCCCAACTCGGAGCTGAGAATCCTTGCTTGCATTCACAAAAACGAAAACATCTATGGCTTGATTCATCTTTTGCACATCACATGTCCAACCCCAGACAACCCCATCTCGGTTTACGTGCTCCATCTGATCGCGCTCGTGGGGCGGTCGAGCCCCGTGTTCATTTCCCACAGACAGGAAGACAAGTCAATTGGGGACCAACCGGCCTACTCAGAAAATGTCATCGTTTCTTTTGAGCACTTGGAGCAGGAAAATTCAGGCGCAGTGTATGCTGAATGCTTCACCACAATCTCACCAAGCAAGTTCATGCACAACGACGTATACAAATTGGCTATGGACAAGTTCACATCCCTCATAATATTGTGTTTCCACAGGACATGGACAAGTGATGGGCTGATAGACCAAGAGGACAACACAATGAGACATTTGAACTGCAGTGTTATTGAGAAAGCGCCTTGCTCGGTGGGCATTTTGGCTGACAAAGGGCGCTTAGGGAGCCTGGCTTCCATGGCATCAGTAGGGCGTGGGAAATGCAAGTATTCAGTCTGTGTGATATTCATGGGAGGGAATGATGATCGAGAGGCCATTTCATTTGCAAAACGAATGGCAAGGGACTCAGAGATTGATCTAACAGTCCTGAAACTTGGGTCGGCAGTGGATGATGGGTCCAGCAAATGGGACAAGATCCTTGACTCTGAGGTAATCAAGGACTTCAAAACGACATTCTTGGGGGATGGGAGGGTGAGGTTCATAGAGGAAGAGAGTGAAGATGGGCCTCAAACAGCGTTGAGACTCAGAGAACTAGTGAATCGATATGATCTGATGATCGTAGGAAGAAGAAAAGGGTTGGAATCATCATCGCCTCAAACATCAGGGCTAAGTGAATGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGGGATTTGATTGCATCACTTGATATCAACACAAGAACTTCTGTCTTGGTAATACAACAACAGAAGTAG

mRNA sequence

ATGTCGCTGATTAAGCATAACTCCACGGAAAATGGGACGAACCAAATTTGTTTGAGTGTGTTGCCTTTCGTAAACTCAGATGGTTTGTGGGCAAAGGCTCATACCTATCAATGGTGGCTCAACAGTTCCTTGCCTCTCTTGGAATTACAGTTGGTTGTGTTCGGTTTGATGATGATAACTATTCAGCTGCTTCTCAAGCGTCTTGGGGCCTCCAAAATTTCCTCTCAAATTTTGACTGGAATGATATTTGGTTGTTCCTGGGGCACGTTTGACGAAGCAAAATTCAAGCTTTTTAGGATTGACAGTGAAGAGGTGTTGGGATTGCTATCATATTTCGGGTACACGCTATATTTGTTCATAACAGCAGTCAAAATGGATATCAGAATGACTATGCAAACAGGCAAGAAAGCATGGATAATTGGAACGCCTTCAGTTCTTGTGCCTCTATTTTGTGGTCTTTTTGTGTGTTCTTTTCTCCACAAAGGCCTAACTGTTGAAGAGATAAGAAAGCTCCCATTAATGACTTCACTGCAAAGTATGATCTCATTTCCAGTGATTGCTTCAGTTTTAAATGAGATGAAAATCGTGAGCACAGAACTTGGTCGTTTGGGGCTGTCCTCAGCATTAGTTGCAGACATATTCAGCCTAATTTCTATGCTAATTACAAAACAACTGACACAAGAGAGTGCTGCAGAAGTGTATTATGCATTTGGAGCATTGTTTGTTCAAGTCTTTTTGGTGTCATTTTTGTTTAGGCCTGCAGTGGTTTGGATTCTGAAACGAACACCTGAAGGGAAGCCAGTGAGCCGTGGTAGTACCCAAGGAGTTTTTGTTTTGGTTCTGCTTTCTTCTATGACATCTACTGTTTTGGGGCACTCAGTAATCTTAGGGCCTTATTTGTTGGGATTGGCCATTCCTGATGGAGGCCCTGTGGGATATTCTTTTGTTGAGAAGATGGAATCCTTTGTTTCAGATTTTTTCGTGCCACTGTTTGCCATTACTTGTGCTCTCAAGGTGGATTTGTCCAAGCTTTTAATAGCATATGGGGGTGCTTTTACAAGAGTTACCGTAATTCTAGCCTTTGTAACTTGTGTAGTCAAATTCTCGTGTGTCTTTCTTGGCTCATTGTATTGTCAGCTGAGCACTAGAGATTCCTTGGTGCTGTCCCTCATTCTCAGCTGCAAGGGTGTTGTTGAGTTGGCCTTTACCTCACTCTACACTGAATACAGAGTTATCAGCAAAGGGACGATGGGGTGGATCACATTTTTCATATTCATTCTTGCAACCTTTGCGCCAATTGGAGTGAAATGCGTGAATGACCTTTCAAGGAAACAAGCAAGCAATCAGAACAGGAGCATCATGCACATGAGCCCCAACTCGGAGCTGAGAATCCTTGCTTGCATTCACAAAAACGAAAACATCTATGGCTTGATTCATCTTTTGCACATCACATGTCCAACCCCAGACAACCCCATCTCGGTTTACGTGCTCCATCTGATCGCGCTCGTGGGGCGGTCGAGCCCCGTGTTCATTTCCCACAGACAGGAAGACAAGTCAATTGGGGACCAACCGGCCTACTCAGAAAATGTCATCGTTTCTTTTGAGCACTTGGAGCAGGAAAATTCAGGCGCAGTGTATGCTGAATGCTTCACCACAATCTCACCAAGCAAGTTCATGCACAACGACGTATACAAATTGGCTATGGACAAGTTCACATCCCTCATAATATTGTGTTTCCACAGGACATGGACAAGTGATGGGCTGATAGACCAAGAGGACAACACAATGAGACATTTGAACTGCAGTGTTATTGAGAAAGCGCCTTGCTCGGTGGGCATTTTGGCTGACAAAGGGCGCTTAGGGAGCCTGGCTTCCATGGCATCAGTAGGGCGTGGGAAATGCAAGTATTCAGTCTGTGTGATATTCATGGGAGGGAATGATGATCGAGAGGCCATTTCATTTGCAAAACGAATGGCAAGGGACTCAGAGATTGATCTAACAGTCCTGAAACTTGGGTCGGCAGTGGATGATGGGTCCAGCAAATGGGACAAGATCCTTGACTCTGAGGTAATCAAGGACTTCAAAACGACATTCTTGGGGGATGGGAGGGTGAGGTTCATAGAGGAAGAGAGTGAAGATGGGCCTCAAACAGCGTTGAGACTCAGAGAACTAGTGAATCGATATGATCTGATGATCGTAGGAAGAAGAAAAGGGTTGGAATCATCATCGCCTCAAACATCAGGGCTAAGTGAATGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGGGATTTGATTGCATCACTTGATATCAACACAAGAACTTCTGTCTTGGTAATACAACAACAGAAGTAG

Coding sequence (CDS)

ATGTCGCTGATTAAGCATAACTCCACGGAAAATGGGACGAACCAAATTTGTTTGAGTGTGTTGCCTTTCGTAAACTCAGATGGTTTGTGGGCAAAGGCTCATACCTATCAATGGTGGCTCAACAGTTCCTTGCCTCTCTTGGAATTACAGTTGGTTGTGTTCGGTTTGATGATGATAACTATTCAGCTGCTTCTCAAGCGTCTTGGGGCCTCCAAAATTTCCTCTCAAATTTTGACTGGAATGATATTTGGTTGTTCCTGGGGCACGTTTGACGAAGCAAAATTCAAGCTTTTTAGGATTGACAGTGAAGAGGTGTTGGGATTGCTATCATATTTCGGGTACACGCTATATTTGTTCATAACAGCAGTCAAAATGGATATCAGAATGACTATGCAAACAGGCAAGAAAGCATGGATAATTGGAACGCCTTCAGTTCTTGTGCCTCTATTTTGTGGTCTTTTTGTGTGTTCTTTTCTCCACAAAGGCCTAACTGTTGAAGAGATAAGAAAGCTCCCATTAATGACTTCACTGCAAAGTATGATCTCATTTCCAGTGATTGCTTCAGTTTTAAATGAGATGAAAATCGTGAGCACAGAACTTGGTCGTTTGGGGCTGTCCTCAGCATTAGTTGCAGACATATTCAGCCTAATTTCTATGCTAATTACAAAACAACTGACACAAGAGAGTGCTGCAGAAGTGTATTATGCATTTGGAGCATTGTTTGTTCAAGTCTTTTTGGTGTCATTTTTGTTTAGGCCTGCAGTGGTTTGGATTCTGAAACGAACACCTGAAGGGAAGCCAGTGAGCCGTGGTAGTACCCAAGGAGTTTTTGTTTTGGTTCTGCTTTCTTCTATGACATCTACTGTTTTGGGGCACTCAGTAATCTTAGGGCCTTATTTGTTGGGATTGGCCATTCCTGATGGAGGCCCTGTGGGATATTCTTTTGTTGAGAAGATGGAATCCTTTGTTTCAGATTTTTTCGTGCCACTGTTTGCCATTACTTGTGCTCTCAAGGTGGATTTGTCCAAGCTTTTAATAGCATATGGGGGTGCTTTTACAAGAGTTACCGTAATTCTAGCCTTTGTAACTTGTGTAGTCAAATTCTCGTGTGTCTTTCTTGGCTCATTGTATTGTCAGCTGAGCACTAGAGATTCCTTGGTGCTGTCCCTCATTCTCAGCTGCAAGGGTGTTGTTGAGTTGGCCTTTACCTCACTCTACACTGAATACAGAGTTATCAGCAAAGGGACGATGGGGTGGATCACATTTTTCATATTCATTCTTGCAACCTTTGCGCCAATTGGAGTGAAATGCGTGAATGACCTTTCAAGGAAACAAGCAAGCAATCAGAACAGGAGCATCATGCACATGAGCCCCAACTCGGAGCTGAGAATCCTTGCTTGCATTCACAAAAACGAAAACATCTATGGCTTGATTCATCTTTTGCACATCACATGTCCAACCCCAGACAACCCCATCTCGGTTTACGTGCTCCATCTGATCGCGCTCGTGGGGCGGTCGAGCCCCGTGTTCATTTCCCACAGACAGGAAGACAAGTCAATTGGGGACCAACCGGCCTACTCAGAAAATGTCATCGTTTCTTTTGAGCACTTGGAGCAGGAAAATTCAGGCGCAGTGTATGCTGAATGCTTCACCACAATCTCACCAAGCAAGTTCATGCACAACGACGTATACAAATTGGCTATGGACAAGTTCACATCCCTCATAATATTGTGTTTCCACAGGACATGGACAAGTGATGGGCTGATAGACCAAGAGGACAACACAATGAGACATTTGAACTGCAGTGTTATTGAGAAAGCGCCTTGCTCGGTGGGCATTTTGGCTGACAAAGGGCGCTTAGGGAGCCTGGCTTCCATGGCATCAGTAGGGCGTGGGAAATGCAAGTATTCAGTCTGTGTGATATTCATGGGAGGGAATGATGATCGAGAGGCCATTTCATTTGCAAAACGAATGGCAAGGGACTCAGAGATTGATCTAACAGTCCTGAAACTTGGGTCGGCAGTGGATGATGGGTCCAGCAAATGGGACAAGATCCTTGACTCTGAGGTAATCAAGGACTTCAAAACGACATTCTTGGGGGATGGGAGGGTGAGGTTCATAGAGGAAGAGAGTGAAGATGGGCCTCAAACAGCGTTGAGACTCAGAGAACTAGTGAATCGATATGATCTGATGATCGTAGGAAGAAGAAAAGGGTTGGAATCATCATCGCCTCAAACATCAGGGCTAAGTGAATGGAATGAGTTTCCAGAGCTTGGAGTTCTTGGGGATTTGATTGCATCACTTGATATCAACACAAGAACTTCTGTCTTGGTAATACAACAACAGAAGTAG

Protein sequence

MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFITAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSMISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGALFVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLNCSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMARDSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK
Homology
BLAST of Carg20358 vs. NCBI nr
Match: KAG7013291.1 (Cation/H(+) antiporter 4, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1526.1 bits (3950), Expect = 0.0e+00
Identity = 783/783 (100.00%), Postives = 783/783 (100.00%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT
Sbjct: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL
Sbjct: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL
Sbjct: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA
Sbjct: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
           FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI
Sbjct: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR
Sbjct: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. NCBI nr
Match: KAG6574224.1 (Cation/H(+) antiporter 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1523.5 bits (3943), Expect = 0.0e+00
Identity = 781/783 (99.74%), Postives = 783/783 (100.00%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT
Sbjct: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL
Sbjct: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL
Sbjct: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTV+LA
Sbjct: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVMLA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
           FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI
Sbjct: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR
Sbjct: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRELVN+YDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LRELVNQYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. NCBI nr
Match: XP_022945776.1 (cation/H(+) antiporter 4-like [Cucurbita moschata])

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 772/783 (98.60%), Postives = 780/783 (99.62%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT
Sbjct: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIAS+LNEMKIVSTELGRLGLSSALVADIFSL+SMLI+KQLT+ESAA+VYYAFGAL
Sbjct: 181 ISFPVIASLLNEMKIVSTELGRLGLSSALVADIFSLLSMLISKQLTEESAAKVYYAFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAV+WILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL
Sbjct: 241 FVQVFLVSFLFRPAVLWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGLAIPDGGPVGYSFVEKMESFVSDFFVP+FAITCALKVDLSKL IAYG AFTRVTVILA
Sbjct: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPMFAITCALKVDLSKLFIAYGAAFTRVTVILA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
           FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI
Sbjct: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSV ILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR
Sbjct: 601 CSVIEKAPCSVAILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRELVN+YDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LRELVNQYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. NCBI nr
Match: XP_023542026.1 (cation/H(+) antiporter 4-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1480.7 bits (3832), Expect = 0.0e+00
Identity = 755/783 (96.42%), Postives = 771/783 (98.47%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           MSLIKHNST NGTNQICLSVLPFVNSDGLWA AH+YQWWLNSSLPLLELQLVVFGLMMIT
Sbjct: 1   MSLIKHNSTANGTNQICLSVLPFVNSDGLWANAHSYQWWLNSSLPLLELQLVVFGLMMIT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQILTGMIFGCSWG FDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQILTGMIFGCSWGAFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMTMQTGKKAWIIG PSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTMQTGKKAWIIGMPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIAS+LNE+KIVSTELGRLGLSSALVADIFSLISML TKQLT+ESAA+VYY+FGAL
Sbjct: 181 ISFPVIASLLNELKIVSTELGRLGLSSALVADIFSLISMLTTKQLTRESAAKVYYSFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAV+WILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL
Sbjct: 241 FVQVFLVSFLFRPAVIWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGLAIPDGGP GYSFVEKMESFVSDFFVPLFAITC+LKVDLSKL IAYG AFTRVTVILA
Sbjct: 301 LGLAIPDGGPAGYSFVEKMESFVSDFFVPLFAITCSLKVDLSKLFIAYGAAFTRVTVILA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
           F+T VVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI
Sbjct: 361 FITSVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIF+LATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFLLATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           L+ITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDK IGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LNITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKPIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTT+SPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTVSPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSV ILADKGRLGSLASMASVGRGKCKYSVCVIF+GG+DDREAISFAKRMAR
Sbjct: 601 CSVIEKAPCSVAILADKGRLGSLASMASVGRGKCKYSVCVIFLGGSDDREAISFAKRMAR 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVR IEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRLIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRE+VN+YDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LREIVNQYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. NCBI nr
Match: XP_022968001.1 (cation/H(+) antiporter 4-like [Cucurbita maxima])

HSP 1 Score: 1447.2 bits (3745), Expect = 0.0e+00
Identity = 738/783 (94.25%), Postives = 761/783 (97.19%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           M LIKHNS  NGTNQIC S+LPFVNSDGLWAK H+Y WWLNSSLPLLELQLVVFGL+M T
Sbjct: 1   MPLIKHNSATNGTNQICFSLLPFVNSDGLWAKVHSYHWWLNSSLPLLELQLVVFGLVMTT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQI+TGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQIMTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMT QTGKKAWIIG PSVLVPLFCGLFVCSFLHKGLTV EIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTKQTGKKAWIIGMPSVLVPLFCGLFVCSFLHKGLTVAEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIAS+LNE+KIVSTELGRLGLSSALVADIFSL S+LITKQLT+ESAA+VYYAFGAL
Sbjct: 181 ISFPVIASLLNELKIVSTELGRLGLSSALVADIFSLTSLLITKQLTRESAAKVYYAFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAV+WILK+TPEGKPVSRGSTQGVFV+VLLSSMTSTVLGHSV LGPYL
Sbjct: 241 FVQVFLVSFLFRPAVLWILKQTPEGKPVSRGSTQGVFVMVLLSSMTSTVLGHSVFLGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGL++PDGGPVG SFVEK+ESFVSDFFVPLFAITCALKVDLSKL IAYG AFTRVTVI+A
Sbjct: 301 LGLSMPDGGPVGSSFVEKVESFVSDFFVPLFAITCALKVDLSKLFIAYGTAFTRVTVIVA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
            +T VVKFSCVFLGSLYCQLS+RDSLVLSLILSCKGVVELAFTSLYTEYRVIS+GTMGWI
Sbjct: 361 IITWVVKFSCVFLGSLYCQLSSRDSLVLSLILSCKGVVELAFTSLYTEYRVISEGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIFILATFAPIGVKC+NDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFILATFAPIGVKCLNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           L+ITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDK IGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LNITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKPIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSV ILADKGRLGSLASMASVGRGKCKYSVCVIFMGG DDREAISFAKRMA 
Sbjct: 601 CSVIEKAPCSVAILADKGRLGSLASMASVGRGKCKYSVCVIFMGGRDDREAISFAKRMAS 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRV FIEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVTFIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRE+VN+YDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LREIVNQYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. ExPASy Swiss-Prot
Match: Q9FYC1 (Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1)

HSP 1 Score: 462.2 bits (1188), Expect = 1.1e-128
Identity = 289/809 (35.72%), Postives = 454/809 (56.12%), Query Frame = 0

Query: 9   TENGTNQICLSVLPF-VNSDGLWAKAH------TYQWWLNSSLPLLELQLVVFGLMMITI 68
           T    N IC  +LP   +S GLW            ++W N   P +++  ++  ++    
Sbjct: 14  TWRDANMIC-GILPINPSSSGLWPSPKLPDPQANIEFW-NYMFPHVQIIFLIVTILWQFF 73

Query: 69  QLLLKRLGASKISSQILTGMIFGCSW--GTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLF 128
              L+RLG  + +S +LTG++   S+        KF       E + GL+    Y ++ F
Sbjct: 74  HFFLRRLGMIRFTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLFGLVGACSYMMFWF 133

Query: 129 ITAVKMDIRMTMQTGKKAWIIGTPSVLVPL-FCGLFVCSFLH-----KGLTVEEIRKLPL 188
           +  VKMD+ +   TG+KA  IG  SVL+ +  C L     L      KG  V    ++  
Sbjct: 134 LMGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIF 193

Query: 189 MTSLQSMISFPVIASVLNEMKIVSTELGRLGLSSALVADIFS--LISMLITKQLTQESAA 248
           +  +Q + SFPVI ++L E+++ ++ELGRL +SSA+++D  +  L ++L+  +  ++  +
Sbjct: 194 IYLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKS 253

Query: 249 EVYYAF--------------GALFVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVF 308
            +   F              G + + V    ++FRP + +I+KRTP G+PV +     + 
Sbjct: 254 RLGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAII 313

Query: 309 VLVLLSSMTSTVLGHSVILGPYLLGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCAL 368
           +LV  S++ +     S+ +GP++LGLA+P G P+G + ++K ES V   F+P F  T A 
Sbjct: 314 ILVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFLPFFVATSAE 373

Query: 369 KVDLSKLLIAYGGAFTRVTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGV 428
           ++D S   I       +  VIL  V+ +VKF+   L +    +  +D + LSLI+S KG+
Sbjct: 374 EIDTS---ILQSWIDLKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGI 433

Query: 429 VELAFTSLYTEYRVISKGTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMS 488
            E        +   I   T   ++ +I + +   P  +K + D SR  A  + R+++HM 
Sbjct: 434 FEFGAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEKRNMLHMK 493

Query: 489 PNSELRILACIHKNENIYGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQED 548
           PNSELRIL+CI+K ++I  +I+LL  TCP+ +NP++ YVLHL+ LVG+++PV ISHR + 
Sbjct: 494 PNSELRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVLISHRLQT 553

Query: 549 KSIGDQPAYSENVIVSFEHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIIL 608
           +   +    SENV+VSFE    +  G+V+   +T +S  K MH D+  LA++  TSLIIL
Sbjct: 554 RKSENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMMHGDICMLALNNTTSLIIL 613

Query: 609 CFHRTWTSDGLIDQEDNTM-RHLNCSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKY 668
            FH+TW++DG     D+ M R LN SV++ +PCSVGI   +   G   ++         Y
Sbjct: 614 PFHQTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGR-RTIKETAANFSSY 673

Query: 669 SVCVIFMGGNDDREAISFAKRMARDSEIDLTVLKLGSAVD--DGSSKWDKILDSEVIKDF 728
            VC++F+GG DDREA+S AKRMARDS I +TV+ L S+    + ++ WD++LD E+++D 
Sbjct: 674 QVCMLFLGGKDDREALSLAKRMARDSRITITVVSLISSEQRANQATDWDRMLDLELLRDV 733

Query: 729 KTTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNE 784
           K+  L    + F EE   D  QT+  L+ + N YDL IVGR KG +S    T GL EW+E
Sbjct: 734 KSNVLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVF--TEGLEEWSE 793

BLAST of Carg20358 vs. ExPASy Swiss-Prot
Match: Q9FFB8 (Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1)

HSP 1 Score: 428.7 bits (1101), Expect = 1.4e-118
Identity = 270/808 (33.42%), Postives = 461/808 (57.05%), Query Frame = 0

Query: 16  ICLSVLPF-VNSDGLWAKAH------TYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRL 75
           IC  VLP   +S+G+W +           +W N + P L++  ++   +   +   L+RL
Sbjct: 22  IC-DVLPINPSSNGVWPQQKFSDPNINVHFW-NYAFPHLQMIFLIISFLWQFLHFFLRRL 81

Query: 76  GASKISSQILTGMIFGCSWGTFDEAKFKLFRID--SEEVLGLLSYFGYTLYLFITAVKMD 135
           G  + +S +LTG++   S+   + A  + F  +   E V  L +   Y ++ F+  VKMD
Sbjct: 82  GMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSLTAACSYMMFWFLMGVKMD 141

Query: 136 IRMTMQTGKKAWIIGTPSVLV-PLFCGLFVCSFLHKGLTVEEIRKL-----PLMTSLQSM 195
             +   TG+KA  IG  SVL+  L C +     L    T      L      ++ S+Q +
Sbjct: 142 TGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIYSIQCL 201

Query: 196 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFS--LISMLITKQLTQESAAEVYYAF- 255
            SFPV+ ++L E+++ ++ELGRL +SSA+++D  +  L S+LI  +  ++    +   F 
Sbjct: 202 SSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTRLGSVFI 261

Query: 256 -------------GALFVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSS 315
                        G + + V +  ++FRP + +I+K+TP G+PV       + V+V  S+
Sbjct: 262 GDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVMVSGSA 321

Query: 316 MTSTVLGHSVILGPYLLGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKL 375
           + +     S+ +GP++LGLA+P G P+G + ++K ES +   F+P F  + + ++D+S L
Sbjct: 322 ILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTEIDISAL 381

Query: 376 LIAYGGAFTRVTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTS 435
              +G       +++   + VVKF    + +L+  +   D   LSLI+S KG+ EL   +
Sbjct: 382 ---FGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFELGAYA 441

Query: 436 LYTEYRVISKGTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRI 495
           L  +   +   T      +I + +   P  ++ + D SR  A  + R++ H+ PNSELRI
Sbjct: 442 LAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHLKPNSELRI 501

Query: 496 LACIHKNENIYGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQP 555
           L+CI++ ++I  +I+LL   CP+ ++P++ YVLHL+ LVG+++P+FISH+ + +   ++ 
Sbjct: 502 LSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQTRRT-EET 561

Query: 556 AYSENVIVSFEHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWT 615
           +YS NV+VSFE   ++  G+V+   +T +S    MH D+  LA++  TSLI+L FH+TW+
Sbjct: 562 SYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILLPFHQTWS 621

Query: 616 SDG-LIDQEDNTMRHLNCSVIEKAPCSVGILADKGRLG--SLASMASVGRGK----CKYS 675
           +DG  +   +N +R+LN SV++ APCSVG+   +   G  +++S      G       Y+
Sbjct: 622 ADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTVPNLSSYN 681

Query: 676 VCVIFMGGNDDREAISFAKRMARDSEIDLTVLKLGSAVDDG--SSKWDKILDSEVIKDFK 735
           +C+IF+GG DDREA++ A RMARD  I++T+++L +  +    ++ WDK+LD E+++D K
Sbjct: 682 ICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKMLDDELLRDVK 741

Query: 736 TTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEF 784
           +  L D  + + E+  ED  +T+  LR +V+ +D+ IVGR  G   +S  T GL EW+EF
Sbjct: 742 SNTLVD--IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNG--RTSVFTEGLEEWSEF 801

BLAST of Carg20358 vs. ExPASy Swiss-Prot
Match: Q9FYB9 (Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2)

HSP 1 Score: 396.7 bits (1018), Expect = 5.9e-109
Identity = 253/773 (32.73%), Postives = 413/773 (53.43%), Query Frame = 0

Query: 24  VNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGASKISSQILTGMIF 83
           ++S G W    +       SLPLLE+Q+++    ++   + L+ +G S+I S ++ G+I 
Sbjct: 17  ISSQGFWENLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGVSQIVSYMIAGLIL 76

Query: 84  GCS-WGTFDEAKFKLF---RIDSEEVLGLLSYFGYTLYLFITAVKMDIRMTMQTGKKAWI 143
           G   +   +++  KL     +D    L  +S FG  ++ F+  V+   R+   +GK   +
Sbjct: 77  GPQLFDILEKSSGKLSADPALDGTAALRCISVFGRLMFTFLMTVRTSRRVAFHSGKLPVV 136

Query: 144 IGTPSVLVPLFCGLFVCSFL-----HKGLTVEEIRKLPLMTSLQSMISFPVIASVLNEMK 203
           IG  S   PLF   F+  F      H     + + +  ++   QS I  P    +L E+K
Sbjct: 137 IGIVSFFAPLFSLSFLNLFTDNIDPHYMSLDKALAERTVIVITQSQILLPSTTYILLELK 196

Query: 204 IVSTELGRLGLSSALVADIFSLISMLI-TKQLT--QESAAEVYYAFGALFVQVFLVSFLF 263
           I+++ELGRL LS++ + D+  + +M++ T Q T    S A  Y    A+ +   +V F+F
Sbjct: 197 IINSELGRLALSASAINDMLGIFAMIVATTQATYIHVSHAIAYRDLVAVIIFFLIVFFVF 256

Query: 264 RPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLGLAIPDGGPV 323
           +P V WI+ RTPE KPV       V +    S+          +LGP ++G+ IP+G P+
Sbjct: 257 KPMVQWIIDRTPEDKPVEDIYIHAVILTAFASAAYFVFFNMKYVLGPLIIGIIIPEGPPL 316

Query: 324 GYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILAFVTCVVKFSCV 383
           G +   K E    + F+P+     A++ D  ++L  +   +    + L  +  V+K    
Sbjct: 317 GSALEAKFERLTMNVFLPISITFSAMRCDGLRILSQFTDIY--FNIFLTLLILVIKLVAC 376

Query: 384 FLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWITFFIFILATFA 443
               LY +L   +SL +SLILS K  VE        E + IS+ T  ++  +  + A   
Sbjct: 377 LTLCLYYKLPRSESLAVSLILSYKSFVEFVLYEAVLEEKFISQATYAFLILYSLLSAGIV 436

Query: 444 PIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHLLHI-TCPTPDN 503
           P+ V+ + D  RK  + Q R I+H+  NS LRIL C+HK EN+   I  L + + P  D 
Sbjct: 437 PMVVRSMYDPKRKYVNYQKRDILHLEANSGLRILTCLHKPENVSETIAFLQLFSSPIHDF 496

Query: 504 PISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQENSGAVYAECF 563
           PI+V VLHL+ LVG+ +P+ +SH ++ K +  + +Y     ++F    QE+  +V    F
Sbjct: 497 PIAVTVLHLVKLVGQINPIIVSHDKKLKRL-HKNSYIHTANLAFRQFMQESLESVTVTTF 556

Query: 564 TTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLNCSVIEKAPCS 623
           T  S    MH D+  LA+D+ TS+I++   R WT DG+ + +D   R LN S++++APCS
Sbjct: 557 TAFSHENLMHEDICTLALDRTTSMIVVPSGRKWTVDGMFESDDLAARQLNQSLLDRAPCS 616

Query: 624 VGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMARDSEIDLTVLK 683
           +GIL D+G+    + + S  + +    V V+F+GG DDREA+S  KRM  +  + +TV++
Sbjct: 617 IGILVDRGQFSRKSYVTS--KNRYNIDVGVLFIGGKDDREALSLVKRMKYNPRVRVTVIR 676

Query: 684 LGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDL 743
           L     +  S+WD ILD+E +KD K+T   +  + + E       +    ++ L   YDL
Sbjct: 677 L-IFDHEIESEWDYILDNEGLKDLKSTESNED-ILYTERIVTSVVEVVKAVQLLAEEYDL 736

Query: 744 MIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK 784
           M+VGR    + +S   SGL+EW E PELGV+GDL+A+ D+N++ SVLV+QQQ+
Sbjct: 737 MVVGRDH--DMTSQDLSGLTEWVELPELGVIGDLLAARDLNSKVSVLVVQQQQ 780

BLAST of Carg20358 vs. ExPASy Swiss-Prot
Match: Q58P69 (Cation/H(+) antiporter 10 OS=Arabidopsis thaliana OX=3702 GN=CHX10 PE=2 SV=2)

HSP 1 Score: 395.2 bits (1014), Expect = 1.7e-108
Identity = 258/779 (33.12%), Postives = 416/779 (53.40%), Query Frame = 0

Query: 24  VNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGASKISSQILTGMIF 83
           ++S G W    +       SLPLLE+Q+++    ++   + L+ +G S+I+S ++ G++ 
Sbjct: 17  ISSQGFWDNLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGISQIASYMIAGIVL 76

Query: 84  GCS-WGTFDEAKFKLF---RIDSEEVLGLLSYFGYTLYLFITAVKMDIRMTMQTGKKAWI 143
           G   +   +++  KL     +D    L  +S FG  ++ F+  V+   R+   +GK   +
Sbjct: 77  GPQLFDVLEKSSGKLSVDPALDGIAALRCISVFGTLMFTFLMTVRTSRRVAFHSGKLPVV 136

Query: 144 IGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSL---------QSMISFPVIASVL 203
           IG  S   PLF GL   +F    +   +   +PL  +L         QS I  P    +L
Sbjct: 137 IGIVSFFAPLF-GLGFQNFFSDNI---DPHYMPLTKALGERTAIVITQSSILLPSTTYIL 196

Query: 204 NEMKIVSTELGRLGLSSALVADIFSLISMLITK-QLT--QESAAEVYYAFGALFVQVFLV 263
            E+KI+++ELGRL LS+ ++ DI  + SM++   Q T    S A  Y    A+ +   +V
Sbjct: 197 LELKIINSELGRLALSACVINDILGIFSMIVASIQATYIHVSHATAYRDTVAVIIFFLVV 256

Query: 264 SFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLGLAIPD 323
             +F+P V W++ RTPE KPV       V +  L S+          ILGP ++G+ IP+
Sbjct: 257 FLVFKPMVQWVIDRTPEDKPVEDMYIHAVIITALASAAYFVFFNMKYILGPLMIGIIIPE 316

Query: 324 GGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILAFVTCVVK 383
           G P+G +   K E    + F+P+     A++ D +++L  +   F    + L F+  V+K
Sbjct: 317 GPPLGSALEAKFERLTMNVFLPISITFSAMRCDGARILSQFNDIF--FNIFLTFLILVIK 376

Query: 384 FSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWITFFIFIL 443
                   LY +L   +SL +S ILS K   +        +   IS+ T  ++  +  + 
Sbjct: 377 LVACLAPCLYYKLPLSESLAVSFILSYKSFADFVLYEAVLDDTYISQATYSFLILYSLLN 436

Query: 444 ATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHLLH-ITCP 503
           A   P  ++ + D  RK  + Q R I+H+  NS+LRIL C+HK EN+   I  L  ++ P
Sbjct: 437 AGIVPTVLRRMYDPRRKYVNYQKRDILHLERNSDLRILTCLHKPENVSETIAFLQLLSSP 496

Query: 504 TPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQENSGAVY 563
             D PI+V VLHL+ LVG+ +P+ +SH ++ K + ++ +Y     ++F     E+  +V 
Sbjct: 497 NLDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRL-NKDSYIHTANLAFRQFVLESLESVT 556

Query: 564 AECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLNCSVIEK 623
              FT  S    MH D+  LA+DK TS+I++   R WT DGL + ++  +RHLN S++++
Sbjct: 557 VTTFTAFSHENLMHEDICTLALDKTTSMIVVPSGRKWTVDGLFESDNTAIRHLNQSLLDR 616

Query: 624 APCSVGILADKGRLGSLASMASVGRGKCKY--SVCVIFMGGNDDREAISFAKRMARDSEI 683
           APCS+GIL D+G+     S  S+   K +Y   V V+F+GG DDREA+S  KRM  +  I
Sbjct: 617 APCSIGILVDRGQF----SRKSIVTSKKRYIIDVGVLFIGGKDDREALSLVKRMKNNPRI 676

Query: 684 DLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALRLREL 743
            +TV++L     +  S WD ILD+E +KD K+T   +  + +IE       +    ++ L
Sbjct: 677 RVTVIRL-VFDHEIESDWDYILDNEGLKDLKST-EDNKDIDYIERIVTSSVEVVKAVQLL 736

Query: 744 VNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK 784
              YDLM+VGR    + +S   SGL EW E PELGV+GDL+A+ D++++ SVLV+QQQ+
Sbjct: 737 AEEYDLMVVGRDH--DMTSQDLSGLMEWVELPELGVIGDLLAARDLSSKVSVLVVQQQQ 780

BLAST of Carg20358 vs. ExPASy Swiss-Prot
Match: Q58P71 (Cation/H(+) antiporter 8 OS=Arabidopsis thaliana OX=3702 GN=CHX8 PE=2 SV=1)

HSP 1 Score: 393.7 bits (1010), Expect = 5.0e-108
Identity = 264/796 (33.17%), Postives = 426/796 (53.52%), Query Frame = 0

Query: 16  ICLSVLPFVNSDGLWAK-----AHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGA 75
           IC    P ++SDG+W K     A  Y W     LP LE+ +++   +     +L K+LG 
Sbjct: 32  ICEEHPPKLSSDGIWEKLIIKSAGLYFW--QYRLPKLEIVILLVFFLWQGFNILFKKLGL 91

Query: 76  S--KISSQILTGMIFGCSWGTFDEAKF---KLFRIDSEEVLGLLSYFGYTLYLFITAVKM 135
           S  K+SS +L G++         E       L   +  +V G L  FG+ ++ F+  V+M
Sbjct: 92  SIPKLSSMMLAGLLLNVLVTLSGENSIIADILVTKNRIDVAGCLGSFGFLIFWFLKGVRM 151

Query: 136 DIRMTMQTGKKAWIIGTPSVLVPLFCGLFVC---SFLHKGLTVEEIRKLPLMTSLQSMIS 195
           D++   +   KA + G  +V  P+  G  +    S  ++ LT +E     +M  ++S+ S
Sbjct: 152 DVKRIFKAEAKARVTGVAAVTFPIVVGFLLFNLKSAKNRPLTFQE---YDVMLLMESITS 211

Query: 196 FPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGALFV 255
           F  IA +L ++ +  + +GR+ LSSALV+DI  L+ ++     +  + A+       + +
Sbjct: 212 FSGIARLLRDLGMNHSSIGRVALSSALVSDIVGLLLLIANVSRSSATLADGLAILTEITL 271

Query: 256 QVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLG 315
            + +   + RP +  I+KR  EG+P+      GV VLV LS M    L     LG + LG
Sbjct: 272 FLVIAFAVVRPIMFKIIKRKGEGRPIEDKYIHGVLVLVCLSCMYWEDLSQFPPLGAFFLG 331

Query: 316 LAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFT--------R 375
           LAIP+G P+G + VE++ESF     +PLF     L+ D +    A+ GA T         
Sbjct: 332 LAIPNGPPIGSALVERLESFNFGIILPLFLTAVMLRTDTT----AWKGALTFFSGDDKKF 391

Query: 376 VTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISK 435
               L  +  ++K S   +     ++  RDS++L+LI+S KG++EL+F       ++++K
Sbjct: 392 AVASLVLLIFLLKLSVSVIVPYLYKMPLRDSIILALIMSHKGIIELSFYLFSLSLKLVTK 451

Query: 436 GTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENI 495
            T   +   I + +   P+ +  + D S++    Q R++  M    EL+ L CIH+ ++I
Sbjct: 452 DTFSILVLSIVLNSLLIPMAIGFLYDPSKQFICYQKRNLASMKNMGELKTLVCIHRPDHI 511

Query: 496 YGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSF 555
             +I+LL  +  + D+P++ YVLHL+ L G+  P  ISH+ +   +G    YSENVI+SF
Sbjct: 512 SSMINLLEASYQSEDSPLTCYVLHLVELRGQDVPTLISHKVQKLGVGAGNKYSENVILSF 571

Query: 556 EHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGL-IDQED 615
           EH  +    ++  + FT I+ +  M +D+  LA+DK  +LIIL FHRTW+ D   I  + 
Sbjct: 572 EHFHRSVCSSISIDTFTCIANANHMQDDICWLALDKAVTLIILPFHRTWSLDRTSIVSDV 631

Query: 616 NTMRHLNCSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAIS 675
             +R LN +V+++APCSVGIL ++  +                 VCVIF+GG DDREA++
Sbjct: 632 EAIRFLNVNVLKQAPCSVGILIERHLVNKKQEPHE------SLKVCVIFVGGKDDREALA 691

Query: 676 FAKRMARDSEIDLTVLK-LGSAVDDGSSKWDKILDSEVIKD-FKTTFLG----DGRVRFI 735
           FAKRMAR   + LTVL+ L S     ++ WD++LD+  +++  K+   G    +    ++
Sbjct: 692 FAKRMARQENVTLTVLRLLASGKSKDATGWDQMLDTVELRELIKSNNAGMVKEETSTIYL 751

Query: 736 EEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIAS 784
           E+E  DG  T++ LR +   YDL +VGR  G   +   T G+  W EF ELGV+GD +AS
Sbjct: 752 EQEILDGADTSMLLRSMAFDYDLFVVGRTCG--ENHEATKGIENWCEFEELGVIGDFLAS 810

BLAST of Carg20358 vs. ExPASy TrEMBL
Match: A0A6J1G1U3 (cation/H(+) antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111449921 PE=4 SV=1)

HSP 1 Score: 1510.4 bits (3909), Expect = 0.0e+00
Identity = 772/783 (98.60%), Postives = 780/783 (99.62%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT
Sbjct: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIAS+LNEMKIVSTELGRLGLSSALVADIFSL+SMLI+KQLT+ESAA+VYYAFGAL
Sbjct: 181 ISFPVIASLLNEMKIVSTELGRLGLSSALVADIFSLLSMLISKQLTEESAAKVYYAFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAV+WILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL
Sbjct: 241 FVQVFLVSFLFRPAVLWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGLAIPDGGPVGYSFVEKMESFVSDFFVP+FAITCALKVDLSKL IAYG AFTRVTVILA
Sbjct: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPMFAITCALKVDLSKLFIAYGAAFTRVTVILA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
           FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI
Sbjct: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSV ILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR
Sbjct: 601 CSVIEKAPCSVAILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRELVN+YDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LRELVNQYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. ExPASy TrEMBL
Match: A0A6J1HTN0 (cation/H(+) antiporter 4-like OS=Cucurbita maxima OX=3661 GN=LOC111467376 PE=4 SV=1)

HSP 1 Score: 1447.2 bits (3745), Expect = 0.0e+00
Identity = 738/783 (94.25%), Postives = 761/783 (97.19%), Query Frame = 0

Query: 1   MSLIKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMIT 60
           M LIKHNS  NGTNQIC S+LPFVNSDGLWAK H+Y WWLNSSLPLLELQLVVFGL+M T
Sbjct: 1   MPLIKHNSATNGTNQICFSLLPFVNSDGLWAKVHSYHWWLNSSLPLLELQLVVFGLVMTT 60

Query: 61  IQLLLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120
           IQLLLKRLGASKISSQI+TGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI
Sbjct: 61  IQLLLKRLGASKISSQIMTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFI 120

Query: 121 TAVKMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSM 180
           TAVKMDIRMT QTGKKAWIIG PSVLVPLFCGLFVCSFLHKGLTV EIRKLPLMTSLQSM
Sbjct: 121 TAVKMDIRMTKQTGKKAWIIGMPSVLVPLFCGLFVCSFLHKGLTVAEIRKLPLMTSLQSM 180

Query: 181 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGAL 240
           ISFPVIAS+LNE+KIVSTELGRLGLSSALVADIFSL S+LITKQLT+ESAA+VYYAFGAL
Sbjct: 181 ISFPVIASLLNELKIVSTELGRLGLSSALVADIFSLTSLLITKQLTRESAAKVYYAFGAL 240

Query: 241 FVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYL 300
           FVQVFLVSFLFRPAV+WILK+TPEGKPVSRGSTQGVFV+VLLSSMTSTVLGHSV LGPYL
Sbjct: 241 FVQVFLVSFLFRPAVLWILKQTPEGKPVSRGSTQGVFVMVLLSSMTSTVLGHSVFLGPYL 300

Query: 301 LGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILA 360
           LGL++PDGGPVG SFVEK+ESFVSDFFVPLFAITCALKVDLSKL IAYG AFTRVTVI+A
Sbjct: 301 LGLSMPDGGPVGSSFVEKVESFVSDFFVPLFAITCALKVDLSKLFIAYGTAFTRVTVIVA 360

Query: 361 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 420
            +T VVKFSCVFLGSLYCQLS+RDSLVLSLILSCKGVVELAFTSLYTEYRVIS+GTMGWI
Sbjct: 361 IITWVVKFSCVFLGSLYCQLSSRDSLVLSLILSCKGVVELAFTSLYTEYRVISEGTMGWI 420

Query: 421 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480
           TFFIFILATFAPIGVKC+NDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL
Sbjct: 421 TFFIFILATFAPIGVKCLNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 480

Query: 481 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 540
           L+ITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDK IGDQPAYSENVIVSFEHLEQE
Sbjct: 481 LNITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKPIGDQPAYSENVIVSFEHLEQE 540

Query: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600
           NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN
Sbjct: 541 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 600

Query: 601 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 660
           CSVIEKAPCSV ILADKGRLGSLASMASVGRGKCKYSVCVIFMGG DDREAISFAKRMA 
Sbjct: 601 CSVIEKAPCSVAILADKGRLGSLASMASVGRGKCKYSVCVIFMGGRDDREAISFAKRMAS 660

Query: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALR 720
           DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRV FIEEESEDGPQTALR
Sbjct: 661 DSEIDLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVTFIEEESEDGPQTALR 720

Query: 721 LRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780
           LRE+VN+YDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ
Sbjct: 721 LREIVNQYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQ 780

Query: 781 QQK 784
           QQK
Sbjct: 781 QQK 783

BLAST of Carg20358 vs. ExPASy TrEMBL
Match: A0A5D3BVZ8 (Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold11G00240 PE=4 SV=1)

HSP 1 Score: 1103.6 bits (2853), Expect = 0.0e+00
Identity = 557/784 (71.05%), Postives = 671/784 (85.59%), Query Frame = 0

Query: 4   IKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQL 63
           ++ NS  N T   C    PFVNSDGLW   H +QWWLN+SLPLLELQLV+F   M  I  
Sbjct: 7   MESNSEFNLTTVTCTFHPPFVNSDGLWTNIHRHQWWLNNSLPLLELQLVLFCFFMAIIHF 66

Query: 64  LLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFITAV 123
           LLKR G SKISSQI+TG+IFGCSWG  D+ KFKLFR++SEE+LGL SYF Y L++FITAV
Sbjct: 67  LLKRSGVSKISSQIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAV 126

Query: 124 KMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSMISF 183
           KMD+ MT++TGK+AWIIG PS+L+PL CGL V SFL +GLT+ EIRKLPLM S+QSMISF
Sbjct: 127 KMDVSMTVKTGKRAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISF 186

Query: 184 PVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQ--LTQESAAEVYYAFGALF 243
           PVIA +LNE+KIVSTELGRLGLSSALVAD+FS  ++ I  Q  +++++A + YY+ G L 
Sbjct: 187 PVIACLLNELKIVSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLC 246

Query: 244 VQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLL 303
           VQVF+VSFLFRPAV+WI+K+TPEGKP SRG+TQ VF++VLLS++TST+LG   ++GPYLL
Sbjct: 247 VQVFMVSFLFRPAVLWIMKQTPEGKPASRGTTQTVFLVVLLSAVTSTLLGQPAVVGPYLL 306

Query: 304 GLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSK-LLIAYGGAFTRVTVILA 363
           GL++ DGGP+G+S +EK+E FVSDFF+P+F ITCAL+VDLS+ LL+A    FTRV +ILA
Sbjct: 307 GLSLTDGGPMGFSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDFTRVNIILA 366

Query: 364 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 423
           FVT V +F C FL SLYCQLS RDSL+LSLIL  KGVVEL+F +L+TEY +IS+G + W 
Sbjct: 367 FVTYVTQFFCTFLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWF 426

Query: 424 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 483
           T F+ ++ATF PI +KC+ND+S+ QASNQNR+IMH+S NSE R+LAC+HKNENIYG IHL
Sbjct: 427 TVFLLLIATFVPIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHL 486

Query: 484 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 543
           L+I+CPT +NP++VY LHLI LVGR++PVFISHR E+K IGDQ  YSEN+++SF+H E++
Sbjct: 487 LNISCPTLENPVAVYALHLIELVGRTTPVFISHRIENKPIGDQ-TYSENMLLSFDHFEKD 546

Query: 544 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 603
           NSG+VYAECFT+ISP KFMHND+ KLAMDK TSLIIL FH TWTSDGLIDQEDNTMR+LN
Sbjct: 547 NSGSVYAECFTSISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLN 606

Query: 604 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 663
           C VIEKAPCSV ILADKG LGS+ASMAS G  KC+Y+VCVI+MGG+DDREAISFAKR+A+
Sbjct: 607 CCVIEKAPCSVAILADKGHLGSIASMASSG-VKCEYTVCVIYMGGSDDREAISFAKRLAK 666

Query: 664 DSEIDLTVLKLGSAVDD-GSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTAL 723
           D +I+LTVLKLGS+V+D G+SKW+K+LDSEVIKDFK T LGDGRV+F+EE SEDGPQTAL
Sbjct: 667 DIKIELTVLKLGSSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTAL 726

Query: 724 RLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVI 783
           RLRELVN +DLMIVGRRKG+E SSPQTSGLSEWNEFPELG+LGDLIASLDINTRTSVLVI
Sbjct: 727 RLRELVNDFDLMIVGRRKGME-SSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVI 786

BLAST of Carg20358 vs. ExPASy TrEMBL
Match: A0A5A7UAF3 (Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold175G00830 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 556/784 (70.92%), Postives = 670/784 (85.46%), Query Frame = 0

Query: 4   IKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQL 63
           ++ NS  N T   C    PFVNSDGLW   H +QWWLN+SLPLLELQLV+F   M  I  
Sbjct: 1   MESNSEFNLTTVTCTFHPPFVNSDGLWTNIHRHQWWLNNSLPLLELQLVLFCFFMAIIHF 60

Query: 64  LLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFITAV 123
           LLKR G SKISSQI+TG+IFGCSWG  D+ KFKLFR++SEE+LGL SYF Y L++FITAV
Sbjct: 61  LLKRSGVSKISSQIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAV 120

Query: 124 KMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSMISF 183
           KMD+ MTM+TGK+AWIIG PS+L+PL CGL V SFL +GLT+ EIRKLPLM S+QSMISF
Sbjct: 121 KMDVSMTMKTGKRAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISF 180

Query: 184 PVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQ--LTQESAAEVYYAFGALF 243
           PVIA +LNE+KIVSTELGRLGLSSALVAD+FS  ++ I  Q  +++++A + YY+ G L 
Sbjct: 181 PVIACLLNELKIVSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLC 240

Query: 244 VQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLL 303
           VQVF+VSFLFRPAV+WI+K+TPEGKP SR +TQ VF++VLLS++TST+LG   ++GPYLL
Sbjct: 241 VQVFMVSFLFRPAVLWIMKQTPEGKPASRSTTQTVFLVVLLSAVTSTLLGQPAVVGPYLL 300

Query: 304 GLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSK-LLIAYGGAFTRVTVILA 363
           GL++ DGGP+G+S +EK+E FVSDFF+P+F ITCAL+VDLS+ LL+A    +TRV +ILA
Sbjct: 301 GLSLTDGGPMGFSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDYTRVNIILA 360

Query: 364 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 423
           FVT V +F C FL SLYCQLS RDSL+LSLIL  KGVVEL+F +L+TEY +IS+G + W 
Sbjct: 361 FVTYVTQFFCTFLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWF 420

Query: 424 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 483
           T F+ ++ATF PI +KC+ND+S+ QASNQNR+IMH+S NSE R+LAC+HKNENIYG IHL
Sbjct: 421 TVFLLLIATFVPIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHL 480

Query: 484 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 543
           L+I+CPT +NP++VY LHLI LVGR++PVFISHR E+K IGDQ  YSEN+++SF+H E++
Sbjct: 481 LNISCPTLENPVAVYALHLIELVGRTTPVFISHRIENKPIGDQ-TYSENMLLSFDHFEKD 540

Query: 544 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 603
           NSG+VYAECFT+ISP KFMHND+ KLAMDK TSLIIL FH TWTSDGLIDQEDNTMR+LN
Sbjct: 541 NSGSVYAECFTSISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLN 600

Query: 604 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 663
           C VIEKAPCSV ILADKG LGS+ASMAS G  KC+Y+VCVI+MGG+DDREAISFAKR+A+
Sbjct: 601 CCVIEKAPCSVAILADKGHLGSIASMASSG-VKCEYTVCVIYMGGSDDREAISFAKRLAK 660

Query: 664 DSEIDLTVLKLGSAVDD-GSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTAL 723
           D +I+LTVLKLGS+V+D G+SKW+K+LDSEVIKDFK T LGDGRV+F+EE SEDGPQTAL
Sbjct: 661 DIKIELTVLKLGSSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTAL 720

Query: 724 RLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVI 783
           RLRELVN +DLMIVGRRKG+E SSPQTSGLSEWNEFPELG+LGDLIASLDINTRTSVLVI
Sbjct: 721 RLRELVNDFDLMIVGRRKGME-SSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVI 780

BLAST of Carg20358 vs. ExPASy TrEMBL
Match: A0A1S4DTU4 (cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103485746 PE=4 SV=1)

HSP 1 Score: 1101.3 bits (2847), Expect = 0.0e+00
Identity = 556/784 (70.92%), Postives = 671/784 (85.59%), Query Frame = 0

Query: 4   IKHNSTENGTNQICLSVLPFVNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQL 63
           ++ NS  N T   C    PFVNSDGLW   + ++WWLN+SLPLLELQLV+F   M  I  
Sbjct: 7   MESNSEFNLTTVTCTFHPPFVNSDGLWTNINRHEWWLNNSLPLLELQLVLFCFFMAIIHF 66

Query: 64  LLKRLGASKISSQILTGMIFGCSWGTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLFITAV 123
           LLKR G SKISSQI+TG+IFGCSWG  D+ KFKLFR++SEE+LGL SYF Y L++FITAV
Sbjct: 67  LLKRSGVSKISSQIITGLIFGCSWGKLDKGKFKLFRVESEEILGLFSYFAYMLFMFITAV 126

Query: 124 KMDIRMTMQTGKKAWIIGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSLQSMISF 183
           KMD+ MTM+TGK+AWIIG PS+L+PL CGL V SFL +GLT+ EIRKLPLM S+QSMISF
Sbjct: 127 KMDVSMTMKTGKRAWIIGLPSILMPLTCGLLVSSFLLEGLTISEIRKLPLMVSMQSMISF 186

Query: 184 PVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQ--LTQESAAEVYYAFGALF 243
           PVIA +LNE+KIVSTELGRLGLSSALVAD+FS  ++ I  Q  +++++A + YY+ G L 
Sbjct: 187 PVIACLLNELKIVSTELGRLGLSSALVADMFSQCAVAIANQIRISRKNAGKGYYSIGGLC 246

Query: 244 VQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLL 303
           VQVF+VSFLFRPAV+WI+K+TPEGKP SRG+TQ VF++VLLS++TST+LG   ++GPYLL
Sbjct: 247 VQVFMVSFLFRPAVLWIMKQTPEGKPASRGTTQTVFLVVLLSAVTSTLLGQPAVVGPYLL 306

Query: 304 GLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSK-LLIAYGGAFTRVTVILA 363
           GL++ DGGP+G+S +EK+E FVSDFF+P+F ITCAL+VDLS+ LL+A    FTRV +ILA
Sbjct: 307 GLSLTDGGPMGFSLIEKLECFVSDFFMPVFVITCALQVDLSQILLVAVVDDFTRVNIILA 366

Query: 364 FVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWI 423
           FVT V +F C FL SLYCQLS RDSL+LSLIL  KGVVEL+F +L+TEY +IS+G + W 
Sbjct: 367 FVTYVTQFFCTFLTSLYCQLSFRDSLMLSLILGSKGVVELSFCTLFTEYNIISRGILAWF 426

Query: 424 TFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHL 483
           T F+ ++ATF PI +KC+ND+S+ QASNQNR+IMH+S NSE R+LAC+HKNENIYG IHL
Sbjct: 427 TVFLLLIATFVPIMLKCLNDISKLQASNQNRNIMHLSQNSEFRVLACVHKNENIYGFIHL 486

Query: 484 LHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQE 543
           L+I+CPT +NP++VY LHLI LVGR++PVFISHR E+K IGDQ  YSEN+++SF+H E++
Sbjct: 487 LNISCPTLENPVAVYALHLIELVGRTTPVFISHRIENKPIGDQ-TYSENMLLSFDHFEKD 546

Query: 544 NSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLN 603
           NSG+VYAECFT+ISP KFMHND+ KLAMDK TSLIIL FH TWTSDGLIDQEDNTMR+LN
Sbjct: 547 NSGSVYAECFTSISPHKFMHNDICKLAMDKITSLIILPFHITWTSDGLIDQEDNTMRNLN 606

Query: 604 CSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMAR 663
           C VIEKAPCSV ILADKG LGS+ASMAS G  KC+Y+VCVI+MGG+DDREAISFAKR+A+
Sbjct: 607 CCVIEKAPCSVAILADKGHLGSIASMASSG-VKCEYTVCVIYMGGSDDREAISFAKRLAK 666

Query: 664 DSEIDLTVLKLGSAVDD-GSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTAL 723
           D +I+LTVLKLGS+V+D G+SKW+K+LDSEVIKDFK T LGDGRV+F+EE SEDGPQTAL
Sbjct: 667 DIKIELTVLKLGSSVEDNGTSKWEKMLDSEVIKDFKMTCLGDGRVKFLEEVSEDGPQTAL 726

Query: 724 RLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVI 783
           RLRELVN +DLMIVGRRKG+E SSPQTSGLSEWNEFPELG+LGDLIASLDINTRTSVLVI
Sbjct: 727 RLRELVNDFDLMIVGRRKGME-SSPQTSGLSEWNEFPELGILGDLIASLDINTRTSVLVI 786

BLAST of Carg20358 vs. TAIR 10
Match: AT3G44900.1 (cation/H+ exchanger 4 )

HSP 1 Score: 462.2 bits (1188), Expect = 8.1e-130
Identity = 289/809 (35.72%), Postives = 454/809 (56.12%), Query Frame = 0

Query: 9   TENGTNQICLSVLPF-VNSDGLWAKAH------TYQWWLNSSLPLLELQLVVFGLMMITI 68
           T    N IC  +LP   +S GLW            ++W N   P +++  ++  ++    
Sbjct: 14  TWRDANMIC-GILPINPSSSGLWPSPKLPDPQANIEFW-NYMFPHVQIIFLIVTILWQFF 73

Query: 69  QLLLKRLGASKISSQILTGMIFGCSW--GTFDEAKFKLFRIDSEEVLGLLSYFGYTLYLF 128
              L+RLG  + +S +LTG++   S+        KF       E + GL+    Y ++ F
Sbjct: 74  HFFLRRLGMIRFTSHMLTGILLSKSFLKENTPARKFLSTEDYKETLFGLVGACSYMMFWF 133

Query: 129 ITAVKMDIRMTMQTGKKAWIIGTPSVLVPL-FCGLFVCSFLH-----KGLTVEEIRKLPL 188
           +  VKMD+ +   TG+KA  IG  SVL+ +  C L     L      KG  V    ++  
Sbjct: 134 LMGVKMDLSLIRSTGRKAVAIGLSSVLLSITVCALIFFLILRDVGTKKGEPVMSFFEIIF 193

Query: 189 MTSLQSMISFPVIASVLNEMKIVSTELGRLGLSSALVADIFS--LISMLITKQLTQESAA 248
           +  +Q + SFPVI ++L E+++ ++ELGRL +SSA+++D  +  L ++L+  +  ++  +
Sbjct: 194 IYLIQCLSSFPVIGNLLFELRLQNSELGRLAMSSAVISDFSTSILSAVLVFLKELKDDKS 253

Query: 249 EVYYAF--------------GALFVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVF 308
            +   F              G + + V    ++FRP + +I+KRTP G+PV +     + 
Sbjct: 254 RLGSVFIGDVIVGNRPMKRAGTVVLFVCFAIYIFRPLMFFIIKRTPSGRPVKKFYIYAII 313

Query: 309 VLVLLSSMTSTVLGHSVILGPYLLGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCAL 368
           +LV  S++ +     S+ +GP++LGLA+P G P+G + ++K ES V   F+P F  T A 
Sbjct: 314 ILVFGSAILADWCKQSIFIGPFILGLAVPHGPPLGSAILQKFESVVFGTFLPFFVATSAE 373

Query: 369 KVDLSKLLIAYGGAFTRVTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGV 428
           ++D S   I       +  VIL  V+ +VKF+   L +    +  +D + LSLI+S KG+
Sbjct: 374 EIDTS---ILQSWIDLKSIVILVSVSFIVKFALTTLPAFLYGMPAKDCIALSLIMSFKGI 433

Query: 429 VELAFTSLYTEYRVISKGTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMS 488
            E        +   I   T   ++ +I + +   P  +K + D SR  A  + R+++HM 
Sbjct: 434 FEFGAYGYAYQRGTIRPVTFTVLSLYILLNSAVIPPLLKRIYDPSRMYAGYEKRNMLHMK 493

Query: 489 PNSELRILACIHKNENIYGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQED 548
           PNSELRIL+CI+K ++I  +I+LL  TCP+ +NP++ YVLHL+ LVG+++PV ISHR + 
Sbjct: 494 PNSELRILSCIYKTDDIRPMINLLEATCPSRENPVATYVLHLMELVGQANPVLISHRLQT 553

Query: 549 KSIGDQPAYSENVIVSFEHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIIL 608
           +   +    SENV+VSFE    +  G+V+   +T +S  K MH D+  LA++  TSLIIL
Sbjct: 554 RKSENMSYNSENVVVSFEQFHNDFFGSVFVSTYTALSVPKMMHGDICMLALNNTTSLIIL 613

Query: 609 CFHRTWTSDGLIDQEDNTM-RHLNCSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKY 668
            FH+TW++DG     D+ M R LN SV++ +PCSVGI   +   G   ++         Y
Sbjct: 614 PFHQTWSADGSAIVSDSLMIRQLNKSVLDLSPCSVGIFVYRSSNGR-RTIKETAANFSSY 673

Query: 669 SVCVIFMGGNDDREAISFAKRMARDSEIDLTVLKLGSAVD--DGSSKWDKILDSEVIKDF 728
            VC++F+GG DDREA+S AKRMARDS I +TV+ L S+    + ++ WD++LD E+++D 
Sbjct: 674 QVCMLFLGGKDDREALSLAKRMARDSRITITVVSLISSEQRANQATDWDRMLDLELLRDV 733

Query: 729 KTTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNE 784
           K+  L    + F EE   D  QT+  L+ + N YDL IVGR KG +S    T GL EW+E
Sbjct: 734 KSNVLAGADIVFSEEVVNDANQTSQLLKSIANEYDLFIVGREKGRKSVF--TEGLEEWSE 793

BLAST of Carg20358 vs. TAIR 10
Match: AT5G22900.1 (cation/H+ exchanger 3 )

HSP 1 Score: 428.7 bits (1101), Expect = 1.0e-119
Identity = 270/808 (33.42%), Postives = 461/808 (57.05%), Query Frame = 0

Query: 16  ICLSVLPF-VNSDGLWAKAH------TYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRL 75
           IC  VLP   +S+G+W +           +W N + P L++  ++   +   +   L+RL
Sbjct: 22  IC-DVLPINPSSNGVWPQQKFSDPNINVHFW-NYAFPHLQMIFLIISFLWQFLHFFLRRL 81

Query: 76  GASKISSQILTGMIFGCSWGTFDEAKFKLFRID--SEEVLGLLSYFGYTLYLFITAVKMD 135
           G  + +S +LTG++   S+   + A  + F  +   E V  L +   Y ++ F+  VKMD
Sbjct: 82  GMIRFTSHMLTGVLLSKSFLKENSAARRFFSTEDYKEIVFSLTAACSYMMFWFLMGVKMD 141

Query: 136 IRMTMQTGKKAWIIGTPSVLV-PLFCGLFVCSFLHKGLTVEEIRKL-----PLMTSLQSM 195
             +   TG+KA  IG  SVL+  L C +     L    T      L      ++ S+Q +
Sbjct: 142 TGLIRTTGRKAITIGLSSVLLSTLVCSVIFFGNLRDVGTKNSDHTLNSLEYVVIYSIQCL 201

Query: 196 ISFPVIASVLNEMKIVSTELGRLGLSSALVADIFS--LISMLITKQLTQESAAEVYYAF- 255
            SFPV+ ++L E+++ ++ELGRL +SSA+++D  +  L S+LI  +  ++    +   F 
Sbjct: 202 SSFPVVGNLLFELRLQNSELGRLAISSAVISDFSTSILASVLIFMKELKDEQTRLGSVFI 261

Query: 256 -------------GALFVQVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSS 315
                        G + + V +  ++FRP + +I+K+TP G+PV       + V+V  S+
Sbjct: 262 GDVIAGNRPLMRAGIVVLFVCIAIYVFRPLMFYIIKQTPSGRPVKAIYLSTIIVMVSGSA 321

Query: 316 MTSTVLGHSVILGPYLLGLAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKL 375
           + +     S+ +GP++LGLA+P G P+G + ++K ES +   F+P F  + + ++D+S L
Sbjct: 322 ILANWCKQSIFMGPFILGLAVPHGPPLGSAIIQKYESAIFGTFLPFFIASSSTEIDISAL 381

Query: 376 LIAYGGAFTRVTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTS 435
              +G       +++   + VVKF    + +L+  +   D   LSLI+S KG+ EL   +
Sbjct: 382 ---FGWEGLNGIILIMVTSFVVKFIFTTVPALFYGMPMEDCFALSLIMSFKGIFELGAYA 441

Query: 436 LYTEYRVISKGTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRI 495
           L  +   +   T      +I + +   P  ++ + D SR  A  + R++ H+ PNSELRI
Sbjct: 442 LAYQRGSVRPETFTVACLYITLNSAIIPPILRYLYDPSRMYAGYEKRNMQHLKPNSELRI 501

Query: 496 LACIHKNENIYGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQP 555
           L+CI++ ++I  +I+LL   CP+ ++P++ YVLHL+ LVG+++P+FISH+ + +   ++ 
Sbjct: 502 LSCIYRTDDISPMINLLEAICPSRESPVATYVLHLMELVGQANPIFISHKLQTRRT-EET 561

Query: 556 AYSENVIVSFEHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWT 615
           +YS NV+VSFE   ++  G+V+   +T +S    MH D+  LA++  TSLI+L FH+TW+
Sbjct: 562 SYSNNVLVSFEKFRKDFYGSVFVSTYTALSMPDTMHGDICMLALNNTTSLILLPFHQTWS 621

Query: 616 SDG-LIDQEDNTMRHLNCSVIEKAPCSVGILADKGRLG--SLASMASVGRGK----CKYS 675
           +DG  +   +N +R+LN SV++ APCSVG+   +   G  +++S      G       Y+
Sbjct: 622 ADGSALISNNNMIRNLNKSVLDVAPCSVGVFVYRSSSGRKNISSGRKTINGTVPNLSSYN 681

Query: 676 VCVIFMGGNDDREAISFAKRMARDSEIDLTVLKLGSAVDDG--SSKWDKILDSEVIKDFK 735
           +C+IF+GG DDREA++ A RMARD  I++T+++L +  +    ++ WDK+LD E+++D K
Sbjct: 682 ICMIFLGGKDDREAVTLATRMARDPRINITIVRLITTDEKARENTVWDKMLDDELLRDVK 741

Query: 736 TTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEF 784
           +  L D  + + E+  ED  +T+  LR +V+ +D+ IVGR  G   +S  T GL EW+EF
Sbjct: 742 SNTLVD--IFYSEKAIEDAAETSSLLRSMVSDFDMFIVGRGNG--RTSVFTEGLEEWSEF 801

BLAST of Carg20358 vs. TAIR 10
Match: AT3G44920.1 (cation/H+ exchanger 11 )

HSP 1 Score: 396.7 bits (1018), Expect = 4.2e-110
Identity = 253/773 (32.73%), Postives = 413/773 (53.43%), Query Frame = 0

Query: 24  VNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGASKISSQILTGMIF 83
           ++S G W    +       SLPLLE+Q+++    ++   + L+ +G S+I S ++ G+I 
Sbjct: 17  ISSQGFWENLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGVSQIVSYMIAGLIL 76

Query: 84  GCS-WGTFDEAKFKLF---RIDSEEVLGLLSYFGYTLYLFITAVKMDIRMTMQTGKKAWI 143
           G   +   +++  KL     +D    L  +S FG  ++ F+  V+   R+   +GK   +
Sbjct: 77  GPQLFDILEKSSGKLSADPALDGTAALRCISVFGRLMFTFLMTVRTSRRVAFHSGKLPVV 136

Query: 144 IGTPSVLVPLFCGLFVCSFL-----HKGLTVEEIRKLPLMTSLQSMISFPVIASVLNEMK 203
           IG  S   PLF   F+  F      H     + + +  ++   QS I  P    +L E+K
Sbjct: 137 IGIVSFFAPLFSLSFLNLFTDNIDPHYMSLDKALAERTVIVITQSQILLPSTTYILLELK 196

Query: 204 IVSTELGRLGLSSALVADIFSLISMLI-TKQLT--QESAAEVYYAFGALFVQVFLVSFLF 263
           I+++ELGRL LS++ + D+  + +M++ T Q T    S A  Y    A+ +   +V F+F
Sbjct: 197 IINSELGRLALSASAINDMLGIFAMIVATTQATYIHVSHAIAYRDLVAVIIFFLIVFFVF 256

Query: 264 RPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLGLAIPDGGPV 323
           +P V WI+ RTPE KPV       V +    S+          +LGP ++G+ IP+G P+
Sbjct: 257 KPMVQWIIDRTPEDKPVEDIYIHAVILTAFASAAYFVFFNMKYVLGPLIIGIIIPEGPPL 316

Query: 324 GYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILAFVTCVVKFSCV 383
           G +   K E    + F+P+     A++ D  ++L  +   +    + L  +  V+K    
Sbjct: 317 GSALEAKFERLTMNVFLPISITFSAMRCDGLRILSQFTDIY--FNIFLTLLILVIKLVAC 376

Query: 384 FLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWITFFIFILATFA 443
               LY +L   +SL +SLILS K  VE        E + IS+ T  ++  +  + A   
Sbjct: 377 LTLCLYYKLPRSESLAVSLILSYKSFVEFVLYEAVLEEKFISQATYAFLILYSLLSAGIV 436

Query: 444 PIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHLLHI-TCPTPDN 503
           P+ V+ + D  RK  + Q R I+H+  NS LRIL C+HK EN+   I  L + + P  D 
Sbjct: 437 PMVVRSMYDPKRKYVNYQKRDILHLEANSGLRILTCLHKPENVSETIAFLQLFSSPIHDF 496

Query: 504 PISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQENSGAVYAECF 563
           PI+V VLHL+ LVG+ +P+ +SH ++ K +  + +Y     ++F    QE+  +V    F
Sbjct: 497 PIAVTVLHLVKLVGQINPIIVSHDKKLKRL-HKNSYIHTANLAFRQFMQESLESVTVTTF 556

Query: 564 TTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLNCSVIEKAPCS 623
           T  S    MH D+  LA+D+ TS+I++   R WT DG+ + +D   R LN S++++APCS
Sbjct: 557 TAFSHENLMHEDICTLALDRTTSMIVVPSGRKWTVDGMFESDDLAARQLNQSLLDRAPCS 616

Query: 624 VGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAISFAKRMARDSEIDLTVLK 683
           +GIL D+G+    + + S  + +    V V+F+GG DDREA+S  KRM  +  + +TV++
Sbjct: 617 IGILVDRGQFSRKSYVTS--KNRYNIDVGVLFIGGKDDREALSLVKRMKYNPRVRVTVIR 676

Query: 684 LGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALRLRELVNRYDL 743
           L     +  S+WD ILD+E +KD K+T   +  + + E       +    ++ L   YDL
Sbjct: 677 L-IFDHEIESEWDYILDNEGLKDLKSTESNED-ILYTERIVTSVVEVVKAVQLLAEEYDL 736

Query: 744 MIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK 784
           M+VGR    + +S   SGL+EW E PELGV+GDL+A+ D+N++ SVLV+QQQ+
Sbjct: 737 MVVGRDH--DMTSQDLSGLTEWVELPELGVIGDLLAARDLNSKVSVLVVQQQQ 780

BLAST of Carg20358 vs. TAIR 10
Match: AT3G44930.1 (cation/H+ exchanger 10 )

HSP 1 Score: 395.2 bits (1014), Expect = 1.2e-109
Identity = 258/779 (33.12%), Postives = 416/779 (53.40%), Query Frame = 0

Query: 24  VNSDGLWAKAHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGASKISSQILTGMIF 83
           ++S G W    +       SLPLLE+Q+++    ++   + L+ +G S+I+S ++ G++ 
Sbjct: 17  ISSQGFWDNLKSPDVVFGYSLPLLEIQIILIFFCIVMSHMFLRCIGISQIASYMIAGIVL 76

Query: 84  GCS-WGTFDEAKFKLF---RIDSEEVLGLLSYFGYTLYLFITAVKMDIRMTMQTGKKAWI 143
           G   +   +++  KL     +D    L  +S FG  ++ F+  V+   R+   +GK   +
Sbjct: 77  GPQLFDVLEKSSGKLSVDPALDGIAALRCISVFGTLMFTFLMTVRTSRRVAFHSGKLPVV 136

Query: 144 IGTPSVLVPLFCGLFVCSFLHKGLTVEEIRKLPLMTSL---------QSMISFPVIASVL 203
           IG  S   PLF GL   +F    +   +   +PL  +L         QS I  P    +L
Sbjct: 137 IGIVSFFAPLF-GLGFQNFFSDNI---DPHYMPLTKALGERTAIVITQSSILLPSTTYIL 196

Query: 204 NEMKIVSTELGRLGLSSALVADIFSLISMLITK-QLT--QESAAEVYYAFGALFVQVFLV 263
            E+KI+++ELGRL LS+ ++ DI  + SM++   Q T    S A  Y    A+ +   +V
Sbjct: 197 LELKIINSELGRLALSACVINDILGIFSMIVASIQATYIHVSHATAYRDTVAVIIFFLVV 256

Query: 264 SFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLGLAIPD 323
             +F+P V W++ RTPE KPV       V +  L S+          ILGP ++G+ IP+
Sbjct: 257 FLVFKPMVQWVIDRTPEDKPVEDMYIHAVIITALASAAYFVFFNMKYILGPLMIGIIIPE 316

Query: 324 GGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFTRVTVILAFVTCVVK 383
           G P+G +   K E    + F+P+     A++ D +++L  +   F    + L F+  V+K
Sbjct: 317 GPPLGSALEAKFERLTMNVFLPISITFSAMRCDGARILSQFNDIF--FNIFLTFLILVIK 376

Query: 384 FSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISKGTMGWITFFIFIL 443
                   LY +L   +SL +S ILS K   +        +   IS+ T  ++  +  + 
Sbjct: 377 LVACLAPCLYYKLPLSESLAVSFILSYKSFADFVLYEAVLDDTYISQATYSFLILYSLLN 436

Query: 444 ATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENIYGLIHLLH-ITCP 503
           A   P  ++ + D  RK  + Q R I+H+  NS+LRIL C+HK EN+   I  L  ++ P
Sbjct: 437 AGIVPTVLRRMYDPRRKYVNYQKRDILHLERNSDLRILTCLHKPENVSETIAFLQLLSSP 496

Query: 504 TPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSFEHLEQENSGAVY 563
             D PI+V VLHL+ LVG+ +P+ +SH ++ K + ++ +Y     ++F     E+  +V 
Sbjct: 497 NLDFPIAVTVLHLVKLVGQINPIIVSHDKKLKRL-NKDSYIHTANLAFRQFVLESLESVT 556

Query: 564 AECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGLIDQEDNTMRHLNCSVIEK 623
              FT  S    MH D+  LA+DK TS+I++   R WT DGL + ++  +RHLN S++++
Sbjct: 557 VTTFTAFSHENLMHEDICTLALDKTTSMIVVPSGRKWTVDGLFESDNTAIRHLNQSLLDR 616

Query: 624 APCSVGILADKGRLGSLASMASVGRGKCKY--SVCVIFMGGNDDREAISFAKRMARDSEI 683
           APCS+GIL D+G+     S  S+   K +Y   V V+F+GG DDREA+S  KRM  +  I
Sbjct: 617 APCSIGILVDRGQF----SRKSIVTSKKRYIIDVGVLFIGGKDDREALSLVKRMKNNPRI 676

Query: 684 DLTVLKLGSAVDDGSSKWDKILDSEVIKDFKTTFLGDGRVRFIEEESEDGPQTALRLREL 743
            +TV++L     +  S WD ILD+E +KD K+T   +  + +IE       +    ++ L
Sbjct: 677 RVTVIRL-VFDHEIESDWDYILDNEGLKDLKST-EDNKDIDYIERIVTSSVEVVKAVQLL 736

Query: 744 VNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIASLDINTRTSVLVIQQQK 784
              YDLM+VGR    + +S   SGL EW E PELGV+GDL+A+ D++++ SVLV+QQQ+
Sbjct: 737 AEEYDLMVVGRDH--DMTSQDLSGLMEWVELPELGVIGDLLAARDLSSKVSVLVVQQQQ 780

BLAST of Carg20358 vs. TAIR 10
Match: AT2G28180.1 (Cation/hydrogen exchanger family protein )

HSP 1 Score: 393.7 bits (1010), Expect = 3.6e-109
Identity = 264/796 (33.17%), Postives = 426/796 (53.52%), Query Frame = 0

Query: 16  ICLSVLPFVNSDGLWAK-----AHTYQWWLNSSLPLLELQLVVFGLMMITIQLLLKRLGA 75
           IC    P ++SDG+W K     A  Y W     LP LE+ +++   +     +L K+LG 
Sbjct: 63  ICEEHPPKLSSDGIWEKLIIKSAGLYFW--QYRLPKLEIVILLVFFLWQGFNILFKKLGL 122

Query: 76  S--KISSQILTGMIFGCSWGTFDEAKF---KLFRIDSEEVLGLLSYFGYTLYLFITAVKM 135
           S  K+SS +L G++         E       L   +  +V G L  FG+ ++ F+  V+M
Sbjct: 123 SIPKLSSMMLAGLLLNVLVTLSGENSIIADILVTKNRIDVAGCLGSFGFLIFWFLKGVRM 182

Query: 136 DIRMTMQTGKKAWIIGTPSVLVPLFCGLFVC---SFLHKGLTVEEIRKLPLMTSLQSMIS 195
           D++   +   KA + G  +V  P+  G  +    S  ++ LT +E     +M  ++S+ S
Sbjct: 183 DVKRIFKAEAKARVTGVAAVTFPIVVGFLLFNLKSAKNRPLTFQE---YDVMLLMESITS 242

Query: 196 FPVIASVLNEMKIVSTELGRLGLSSALVADIFSLISMLITKQLTQESAAEVYYAFGALFV 255
           F  IA +L ++ +  + +GR+ LSSALV+DI  L+ ++     +  + A+       + +
Sbjct: 243 FSGIARLLRDLGMNHSSIGRVALSSALVSDIVGLLLLIANVSRSSATLADGLAILTEITL 302

Query: 256 QVFLVSFLFRPAVVWILKRTPEGKPVSRGSTQGVFVLVLLSSMTSTVLGHSVILGPYLLG 315
            + +   + RP +  I+KR  EG+P+      GV VLV LS M    L     LG + LG
Sbjct: 303 FLVIAFAVVRPIMFKIIKRKGEGRPIEDKYIHGVLVLVCLSCMYWEDLSQFPPLGAFFLG 362

Query: 316 LAIPDGGPVGYSFVEKMESFVSDFFVPLFAITCALKVDLSKLLIAYGGAFT--------R 375
           LAIP+G P+G + VE++ESF     +PLF     L+ D +    A+ GA T         
Sbjct: 363 LAIPNGPPIGSALVERLESFNFGIILPLFLTAVMLRTDTT----AWKGALTFFSGDDKKF 422

Query: 376 VTVILAFVTCVVKFSCVFLGSLYCQLSTRDSLVLSLILSCKGVVELAFTSLYTEYRVISK 435
               L  +  ++K S   +     ++  RDS++L+LI+S KG++EL+F       ++++K
Sbjct: 423 AVASLVLLIFLLKLSVSVIVPYLYKMPLRDSIILALIMSHKGIIELSFYLFSLSLKLVTK 482

Query: 436 GTMGWITFFIFILATFAPIGVKCVNDLSRKQASNQNRSIMHMSPNSELRILACIHKNENI 495
            T   +   I + +   P+ +  + D S++    Q R++  M    EL+ L CIH+ ++I
Sbjct: 483 DTFSILVLSIVLNSLLIPMAIGFLYDPSKQFICYQKRNLASMKNMGELKTLVCIHRPDHI 542

Query: 496 YGLIHLLHITCPTPDNPISVYVLHLIALVGRSSPVFISHRQEDKSIGDQPAYSENVIVSF 555
             +I+LL  +  + D+P++ YVLHL+ L G+  P  ISH+ +   +G    YSENVI+SF
Sbjct: 543 SSMINLLEASYQSEDSPLTCYVLHLVELRGQDVPTLISHKVQKLGVGAGNKYSENVILSF 602

Query: 556 EHLEQENSGAVYAECFTTISPSKFMHNDVYKLAMDKFTSLIILCFHRTWTSDGL-IDQED 615
           EH  +    ++  + FT I+ +  M +D+  LA+DK  +LIIL FHRTW+ D   I  + 
Sbjct: 603 EHFHRSVCSSISIDTFTCIANANHMQDDICWLALDKAVTLIILPFHRTWSLDRTSIVSDV 662

Query: 616 NTMRHLNCSVIEKAPCSVGILADKGRLGSLASMASVGRGKCKYSVCVIFMGGNDDREAIS 675
             +R LN +V+++APCSVGIL ++  +                 VCVIF+GG DDREA++
Sbjct: 663 EAIRFLNVNVLKQAPCSVGILIERHLVNKKQEPHE------SLKVCVIFVGGKDDREALA 722

Query: 676 FAKRMARDSEIDLTVLK-LGSAVDDGSSKWDKILDSEVIKD-FKTTFLG----DGRVRFI 735
           FAKRMAR   + LTVL+ L S     ++ WD++LD+  +++  K+   G    +    ++
Sbjct: 723 FAKRMARQENVTLTVLRLLASGKSKDATGWDQMLDTVELRELIKSNNAGMVKEETSTIYL 782

Query: 736 EEESEDGPQTALRLRELVNRYDLMIVGRRKGLESSSPQTSGLSEWNEFPELGVLGDLIAS 784
           E+E  DG  T++ LR +   YDL +VGR  G   +   T G+  W EF ELGV+GD +AS
Sbjct: 783 EQEILDGADTSMLLRSMAFDYDLFVVGRTCG--ENHEATKGIENWCEFEELGVIGDFLAS 841

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7013291.10.0e+00100.00Cation/H(+) antiporter 4, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6574224.10.0e+0099.74Cation/H(+) antiporter 4, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022945776.10.0e+0098.60cation/H(+) antiporter 4-like [Cucurbita moschata][more]
XP_023542026.10.0e+0096.42cation/H(+) antiporter 4-like [Cucurbita pepo subsp. pepo][more]
XP_022968001.10.0e+0094.25cation/H(+) antiporter 4-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q9FYC11.1e-12835.72Cation/H(+) antiporter 4 OS=Arabidopsis thaliana OX=3702 GN=CHX4 PE=2 SV=1[more]
Q9FFB81.4e-11833.42Cation/H(+) antiporter 3 OS=Arabidopsis thaliana OX=3702 GN=CHX3 PE=2 SV=1[more]
Q9FYB95.9e-10932.73Cation/H(+) antiporter 11 OS=Arabidopsis thaliana OX=3702 GN=CHX11 PE=2 SV=2[more]
Q58P691.7e-10833.12Cation/H(+) antiporter 10 OS=Arabidopsis thaliana OX=3702 GN=CHX10 PE=2 SV=2[more]
Q58P715.0e-10833.17Cation/H(+) antiporter 8 OS=Arabidopsis thaliana OX=3702 GN=CHX8 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1G1U30.0e+0098.60cation/H(+) antiporter 4-like OS=Cucurbita moschata OX=3662 GN=LOC111449921 PE=4... [more]
A0A6J1HTN00.0e+0094.25cation/H(+) antiporter 4-like OS=Cucurbita maxima OX=3661 GN=LOC111467376 PE=4 S... [more]
A0A5D3BVZ80.0e+0071.05Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_sc... [more]
A0A5A7UAF30.0e+0070.92Cation/H(+) antiporter 4-like OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S4DTU40.0e+0070.92cation/H(+) antiporter 4-like OS=Cucumis melo OX=3656 GN=LOC103485746 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT3G44900.18.1e-13035.72cation/H+ exchanger 4 [more]
AT5G22900.11.0e-11933.42cation/H+ exchanger 3 [more]
AT3G44920.14.2e-11032.73cation/H+ exchanger 11 [more]
AT3G44930.11.2e-10933.12cation/H+ exchanger 10 [more]
AT2G28180.13.6e-10933.17Cation/hydrogen exchanger family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR038770Sodium/solute symporter superfamilyGENE3D1.20.1530.20coord: 46..449
e-value: 1.4E-51
score: 177.5
IPR006153Cation/H+ exchangerPFAMPF00999Na_H_Exchangercoord: 53..436
e-value: 2.8E-35
score: 121.8
NoneNo IPR availablePANTHERPTHR32468CATION/H + ANTIPORTERcoord: 11..783
NoneNo IPR availablePANTHERPTHR32468:SF17CATION/H(+) ANTIPORTER 4coord: 11..783

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20358-RACarg20358-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:1902600 proton transmembrane transport
biological_process GO:0006885 regulation of pH
biological_process GO:0006812 cation transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0012505 endomembrane system
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0015299 solute:proton antiporter activity