Carg20243 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20243
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Descriptiondof zinc finger protein DOF3.2-like
LocationCarg_Chr02: 4734325 .. 4735202 (+)
RNA-Seq ExpressionCarg20243
SyntenyCarg20243
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
TTTATCTCCCTGCGTTACTCCTTTCTCAACAAACCTCTCTCTCTCTCTCTATAGCAAATACAGAACTCAGTCTTCCAACCTTCATCCATTCGCTGGTTTTATGAATGGATCATTCAAGCGGCCAACAAGTAAGGATTAGAATGGTTTTTGTTCAAGTTCTCAAGATATCCATTAATTTCAACTCCTGATTTGTTTGTTTAATTTTACTTCAGGAAGCTCAAGCCCGACCACTGGAGGACATGGTGGGCGTGATTTCATGTCCAAAAGTGCAGCAAGAAAACAGGAAGCCAATGAGGCCACAGCCAGAGCAAGTCCTGAGATGCCCAAGATGTGATTCTACCAACACCAAGTTCTGCTACTACAATAACTACAGTCTCTCCCAGCCAAGGTACTTTTGCAAGTCCTGTAGAAGGTATTGGACTAAAGGGGGAACTCTAAGAAATGTTCCTGTGGGTGGCTCTTGCAGGAAGAACAAAAGACCTTCACCTTCTACTTCATCTTCTTCCTCTCCATCTCCATCTTCAAAGAGATCACATGATCAGGTCGCCAATTCTAGCTATGCTAAGCTTCTAAATCAATCAAATGGAAGCATGGGTCTTTTTGATACCCACAGCAACTATCATAATATGTACAGTGGATTTGGTAATGGAAGCTCTGGGGAGGTTGAACATGGGTCTACAGAGACTGCTACAGCAATGAAGCAAGAGTTGCTACCCAATGGAGATCAAAATATGGTTTGTTGGGGATTTCCATGGCAGTTCAACAATGGGGATGGAAATTACATGGGTGATCTTGATTCTGGAAGAGAAAGCTGGAGTTTCAATGGAAATGGAATTGCTTCATCTTGGCATGGGCTTCTCAACAGTCCTCTAATGTAA

mRNA sequence

TTTATCTCCCTGCGTTACTCCTTTCTCAACAAACCTCTCTCTCTCTCTCTATAGCAAATACAGAACTCAGTCTTCCAACCTTCATCCATTCGCTGGTTTTATGAATGGATCATTCAAGCGGCCAACAAGAAGCTCAAGCCCGACCACTGGAGGACATGGTGGGCGTGATTTCATGTCCAAAAGTGCAGCAAGAAAACAGGAAGCCAATGAGGCCACAGCCAGAGCAAGTCCTGAGATGCCCAAGATGTGATTCTACCAACACCAAGTTCTGCTACTACAATAACTACAGTCTCTCCCAGCCAAGGTACTTTTGCAAGTCCTGTAGAAGGTATTGGACTAAAGGGGGAACTCTAAGAAATGTTCCTGTGGGTGGCTCTTGCAGGAAGAACAAAAGACCTTCACCTTCTACTTCATCTTCTTCCTCTCCATCTCCATCTTCAAAGAGATCACATGATCAGGTCGCCAATTCTAGCTATGCTAAGCTTCTAAATCAATCAAATGGAAGCATGGGTCTTTTTGATACCCACAGCAACTATCATAATATGTACAGTGGATTTGGTAATGGAAGCTCTGGGGAGGTTGAACATGGGTCTACAGAGACTGCTACAGCAATGAAGCAAGAGTTGCTACCCAATGGAGATCAAAATATGGTTTGTTGGGGATTTCCATGGCAGTTCAACAATGGGGATGGAAATTACATGGGTGATCTTGATTCTGGAAGAGAAAGCTGGAGTTTCAATGGAAATGGAATTGCTTCATCTTGGCATGGGCTTCTCAACAGTCCTCTAATGTAA

Coding sequence (CDS)

ATGGATCATTCAAGCGGCCAACAAGAAGCTCAAGCCCGACCACTGGAGGACATGGTGGGCGTGATTTCATGTCCAAAAGTGCAGCAAGAAAACAGGAAGCCAATGAGGCCACAGCCAGAGCAAGTCCTGAGATGCCCAAGATGTGATTCTACCAACACCAAGTTCTGCTACTACAATAACTACAGTCTCTCCCAGCCAAGGTACTTTTGCAAGTCCTGTAGAAGGTATTGGACTAAAGGGGGAACTCTAAGAAATGTTCCTGTGGGTGGCTCTTGCAGGAAGAACAAAAGACCTTCACCTTCTACTTCATCTTCTTCCTCTCCATCTCCATCTTCAAAGAGATCACATGATCAGGTCGCCAATTCTAGCTATGCTAAGCTTCTAAATCAATCAAATGGAAGCATGGGTCTTTTTGATACCCACAGCAACTATCATAATATGTACAGTGGATTTGGTAATGGAAGCTCTGGGGAGGTTGAACATGGGTCTACAGAGACTGCTACAGCAATGAAGCAAGAGTTGCTACCCAATGGAGATCAAAATATGGTTTGTTGGGGATTTCCATGGCAGTTCAACAATGGGGATGGAAATTACATGGGTGATCTTGATTCTGGAAGAGAAAGCTGGAGTTTCAATGGAAATGGAATTGCTTCATCTTGGCATGGGCTTCTCAACAGTCCTCTAATGTAA

Protein sequence

MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVANSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Homology
BLAST of Carg20243 vs. NCBI nr
Match: KAG7035454.1 (Dof zinc finger protein DOF3.2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 473.4 bits (1217), Expect = 1.1e-129
Identity = 229/229 (100.00%), Postives = 229/229 (100.00%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60
           MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN
Sbjct: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60

Query: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120
           YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA
Sbjct: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120

Query: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180
           NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ
Sbjct: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180

Query: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 229

BLAST of Carg20243 vs. NCBI nr
Match: XP_022946981.1 (dof zinc finger protein DOF3.2-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 461.5 bits (1186), Expect = 4.4e-126
Identity = 224/229 (97.82%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60
           MDHSSGQQEAQARPLEDMVGVISCPKVQQENRK MRPQPEQVLRCPRCDSTNTKFCYYNN
Sbjct: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKAMRPQPEQVLRCPRCDSTNTKFCYYNN 60

Query: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120
           YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ A
Sbjct: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAA 120

Query: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180
           NSSYAKLLNQSNG MGLFDTH NYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ
Sbjct: 121 NSSYAKLLNQSNGRMGLFDTHKNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180

Query: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           NMV WGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 181 NMVSWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 229

BLAST of Carg20243 vs. NCBI nr
Match: XP_023532294.1 (dof zinc finger protein DOF3.2-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 453.4 bits (1165), Expect = 1.2e-123
Identity = 219/229 (95.63%), Postives = 225/229 (98.25%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60
           MDHSSGQQEA+A+PLEDMVG+ISCPKVQQENRK MRPQPEQVLRCPRCDSTNTKFCYYNN
Sbjct: 1   MDHSSGQQEAEAQPLEDMVGMISCPKVQQENRKAMRPQPEQVLRCPRCDSTNTKFCYYNN 60

Query: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120
           YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRS+DQ A
Sbjct: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSYDQAA 120

Query: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180
           NSSYAKLLNQSNGSMGLFDTH+NYHNMYSGFGNGSSGEVEH STETATAMKQELLP+GDQ
Sbjct: 121 NSSYAKLLNQSNGSMGLFDTHNNYHNMYSGFGNGSSGEVEHASTETATAMKQELLPSGDQ 180

Query: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           NMV WGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 181 NMVSWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 229

BLAST of Carg20243 vs. NCBI nr
Match: XP_023007417.1 (dof zinc finger protein DOF2.1-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 450.3 bits (1157), Expect = 1.0e-122
Identity = 218/229 (95.20%), Postives = 222/229 (96.94%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60
           MDHSSGQQEAQA+PLEDMV +ISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN
Sbjct: 1   MDHSSGQQEAQAQPLEDMVDMISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60

Query: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120
           YSLSQPR+FCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ A
Sbjct: 61  YSLSQPRFFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAA 120

Query: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180
           NSSYAKLLNQSNG MGLFDTH+NYHNMYSG GNGSSGEVEH STETATAMKQELLPNGDQ
Sbjct: 121 NSSYAKLLNQSNGRMGLFDTHNNYHNMYSGLGNGSSGEVEHASTETATAMKQELLPNGDQ 180

Query: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           NMV WGFPWQFNN DGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 181 NMVSWGFPWQFNNEDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 229

BLAST of Carg20243 vs. NCBI nr
Match: XP_022946980.1 (dof zinc finger protein DOF3.2-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 446.8 bits (1148), Expect = 1.1e-121
Identity = 217/222 (97.75%), Postives = 217/222 (97.75%), Query Frame = 0

Query: 8   QEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR 67
           QEAQARPLEDMVGVISCPKVQQENRK MRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR
Sbjct: 9   QEAQARPLEDMVGVISCPKVQQENRKAMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR 68

Query: 68  YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVANSSYAKL 127
           YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ ANSSYAKL
Sbjct: 69  YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAANSSYAKL 128

Query: 128 LNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMVCWGF 187
           LNQSNG MGLFDTH NYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMV WGF
Sbjct: 129 LNQSNGRMGLFDTHKNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMVSWGF 188

Query: 188 PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 189 PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230

BLAST of Carg20243 vs. ExPASy Swiss-Prot
Match: Q9M1E6 (Dof zinc finger protein DOF3.2 OS=Arabidopsis thaliana OX=3702 GN=DOF3.2 PE=1 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 3.3e-36
Identity = 107/247 (43.32%), Postives = 135/247 (54.66%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQEN-RKPM---RP-QPEQVLRCPRCDSTNTKF 60
           MD+SS  Q        +++GV SC     +N +KP+   RP  PEQ LRCPRCDSTNTKF
Sbjct: 1   MDYSSMHQ--------NVMGVSSCSTQDYQNQKKPLSATRPAPPEQSLRCPRCDSTNTKF 60

Query: 61  CYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSS-SSSPSPSSKR 120
           CYYNNYSLSQPRYFCKSCRRYWTKGG LRN+P+GG+ RK+KR S +T S  ++P P+   
Sbjct: 61  CYYNNYSLSQPRYFCKSCRRYWTKGGILRNIPIGGAYRKHKRSSSATKSLRTTPEPTMTH 120

Query: 121 SHDQVANSSYAKLLNQ-SNGSMGLFDTHSNYH------NMYSGFGNGSS--------GEV 180
                  +S+    N  SN  M L   ++  +      + + GFG+  S        G  
Sbjct: 121 DGKSFPTASFGYNNNNISNEQMELGLAYALLNKQPLGVSSHLGFGSSQSPMAMDGVYGTT 180

Query: 181 EHGSTETATAM-----KQELLPNGDQNMVCWGFPWQFNNGDGN-----YMGDLDSGRESW 217
            H    T  A        E +   D N V WGFPWQ N G G+     ++  +DSGRE W
Sbjct: 181 SHQMENTGYAFGNGGGGMEQMATSDPNRVLWGFPWQMNMGGGSGHGHGHVDQIDSGREIW 239

BLAST of Carg20243 vs. ExPASy Swiss-Prot
Match: Q84TE9 (Dof zinc finger protein DOF5.3 OS=Arabidopsis thaliana OX=3702 GN=DOF5.3 PE=2 SV=1)

HSP 1 Score: 150.2 bits (378), Expect = 2.8e-35
Identity = 98/220 (44.55%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 36  RPQ-PEQVLRCPRCDSTNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRK 95
           RPQ PE  LRCPRCDSTNTKFCYYNNYSL+QPRYFCKSCRRYWTKGGTLRN+PVGG CRK
Sbjct: 47  RPQPPELALRCPRCDSTNTKFCYYNNYSLTQPRYFCKSCRRYWTKGGTLRNIPVGGGCRK 106

Query: 96  NKRPSPSTSSSSSPSPSSKRSHDQVANS-----------------SYAKLLNQSNGSM-- 155
           NKR + S + S   +P    SHD    S                 ++A L  Q  GS   
Sbjct: 107 NKRSTSSAARSLRTTP-EPASHDGKVFSAAGFNGYSNNEHIDLSLAFALLNKQHPGSSSQ 166

Query: 156 ----------------GLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGD 211
                           G+F T     N    FGNGSSG                    GD
Sbjct: 167 LGFHSELGSSHQSDMEGMFGTSQQKENATYAFGNGSSG-------------------LGD 226

BLAST of Carg20243 vs. ExPASy Swiss-Prot
Match: Q8LE43 (Dof zinc finger protein DOF2.1 OS=Arabidopsis thaliana OX=3702 GN=DOF2.1 PE=2 SV=2)

HSP 1 Score: 147.1 bits (370), Expect = 2.4e-34
Identity = 107/291 (36.77%), Postives = 136/291 (46.74%), Query Frame = 0

Query: 7   QQEAQARPLEDMVGVISCPKVQQENRKPMRPQPE----------QVLRCPRCDSTNTKFC 66
           +QE     LE +  ++S  K    N K M  + +          +   CPRC+S NTKFC
Sbjct: 4   EQEISNETLETI--LVSSTKGSNNNNKKMEEEMKKKVSRGELGGEAQNCPRCESPNTKFC 63

Query: 67  YYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSS--------- 126
           YYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGG CR+NKR S S  S ++         
Sbjct: 64  YYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRRNKRSSSSAFSKNNNNKSINFHT 123

Query: 127 -----------PSPSSKRSHDQVANSSYAKL----------------------LNQSNGS 186
                      P  S    H    N ++A L                         S   
Sbjct: 124 DPLQNPLITGMPPSSFGYDHSIDLNLAFATLQKHHLSSQATTPSFGFGGDLSIYGNSTND 183

Query: 187 MGLFDTHSNYHN------MYSGFGNGSSGEVEHGST----ETATAMKQELLPNGDQN--- 230
           +G+F   +  +N        SG GN +  E++  ST          KQE + N + N   
Sbjct: 184 VGIFGGQNGTYNNSLCYGFMSGNGNNNQNEIKMASTLGMSLEGNERKQENVNNNNNNSEN 243

BLAST of Carg20243 vs. ExPASy Swiss-Prot
Match: Q9FM03 (Dof zinc finger protein DOF5.6 OS=Arabidopsis thaliana OX=3702 GN=DOF5.6 PE=2 SV=2)

HSP 1 Score: 132.1 bits (331), Expect = 7.9e-30
Identity = 79/156 (50.64%), Postives = 97/156 (62.18%), Query Frame = 0

Query: 13  RPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPRYFCKS 72
           RP    V V +   +    R  +RP  +   +CPRC+ST+TKFCYYNNYSLSQPRYFCK+
Sbjct: 45  RPQASAVAVAAAALMDGGRR--LRPPHDHPQKCPRCESTHTKFCYYNNYSLSQPRYFCKT 104

Query: 73  CRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVANSSYAKLLNQSN 132
           CRRYWTKGGTLRN+PVGG CRKNK+PS S++SSSS S   K S+   AN+S         
Sbjct: 105 CRRYWTKGGTLRNIPVGGGCRKNKKPS-SSNSSSSTSSGKKPSNIVTANTS--------- 164

Query: 133 GSMGLFDTHSNYHNMYSGFGN-----GSSGEVEHGS 164
             M L  +H NY +   GF +     GS    EHG+
Sbjct: 165 DLMALAHSHQNYQHSPLGFSHFGGMMGSYSTPEHGN 188

BLAST of Carg20243 vs. ExPASy Swiss-Prot
Match: Q84JQ8 (Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV=1)

HSP 1 Score: 127.1 bits (318), Expect = 2.6e-28
Identity = 71/130 (54.62%), Postives = 85/130 (65.38%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKP-------MRPQPEQVLRCPRCDSTNT 60
           MD +   QE   +P+ ++V   +C K Q     P        RP+ +Q L CPRC+S NT
Sbjct: 1   MDTAKWPQEFVVKPMNEIV-TNTCLKQQSNPPSPATPVERKARPEKDQALNCPRCNSLNT 60

Query: 61  KFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSK 120
           KFCYYNNYSL+QPRYFCK CRRYWT GG+LRN+PVGG  RKNKR S S SSSSSPS SS 
Sbjct: 61  KFCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGGGVRKNKR-SSSNSSSSSPSSSSS 120

Query: 121 RSHDQVANSS 124
                 AN++
Sbjct: 121 SKKPLFANNN 128

BLAST of Carg20243 vs. ExPASy TrEMBL
Match: A0A6J1G548 (dof zinc finger protein DOF3.2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111450998 PE=4 SV=1)

HSP 1 Score: 461.5 bits (1186), Expect = 2.1e-126
Identity = 224/229 (97.82%), Postives = 224/229 (97.82%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60
           MDHSSGQQEAQARPLEDMVGVISCPKVQQENRK MRPQPEQVLRCPRCDSTNTKFCYYNN
Sbjct: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKAMRPQPEQVLRCPRCDSTNTKFCYYNN 60

Query: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120
           YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ A
Sbjct: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAA 120

Query: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180
           NSSYAKLLNQSNG MGLFDTH NYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ
Sbjct: 121 NSSYAKLLNQSNGRMGLFDTHKNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180

Query: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           NMV WGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 181 NMVSWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 229

BLAST of Carg20243 vs. ExPASy TrEMBL
Match: A0A6J1KYM5 (dof zinc finger protein DOF2.1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111499920 PE=4 SV=1)

HSP 1 Score: 450.3 bits (1157), Expect = 4.9e-123
Identity = 218/229 (95.20%), Postives = 222/229 (96.94%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60
           MDHSSGQQEAQA+PLEDMV +ISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN
Sbjct: 1   MDHSSGQQEAQAQPLEDMVDMISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNN 60

Query: 61  YSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVA 120
           YSLSQPR+FCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ A
Sbjct: 61  YSLSQPRFFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAA 120

Query: 121 NSSYAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQ 180
           NSSYAKLLNQSNG MGLFDTH+NYHNMYSG GNGSSGEVEH STETATAMKQELLPNGDQ
Sbjct: 121 NSSYAKLLNQSNGRMGLFDTHNNYHNMYSGLGNGSSGEVEHASTETATAMKQELLPNGDQ 180

Query: 181 NMVCWGFPWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           NMV WGFPWQFNN DGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 181 NMVSWGFPWQFNNEDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 229

BLAST of Carg20243 vs. ExPASy TrEMBL
Match: A0A6J1G5J2 (dof zinc finger protein DOF3.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111450998 PE=4 SV=1)

HSP 1 Score: 446.8 bits (1148), Expect = 5.4e-122
Identity = 217/222 (97.75%), Postives = 217/222 (97.75%), Query Frame = 0

Query: 8   QEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR 67
           QEAQARPLEDMVGVISCPKVQQENRK MRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR
Sbjct: 9   QEAQARPLEDMVGVISCPKVQQENRKAMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR 68

Query: 68  YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVANSSYAKL 127
           YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ ANSSYAKL
Sbjct: 69  YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAANSSYAKL 128

Query: 128 LNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMVCWGF 187
           LNQSNG MGLFDTH NYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMV WGF
Sbjct: 129 LNQSNGRMGLFDTHKNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMVSWGF 188

Query: 188 PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 189 PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230

BLAST of Carg20243 vs. ExPASy TrEMBL
Match: A0A6J1L0G7 (dof zinc finger protein DOF2.1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111499920 PE=4 SV=1)

HSP 1 Score: 435.6 bits (1119), Expect = 1.2e-118
Identity = 211/222 (95.05%), Postives = 215/222 (96.85%), Query Frame = 0

Query: 8   QEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR 67
           QEAQA+PLEDMV +ISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR
Sbjct: 9   QEAQAQPLEDMVDMISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPR 68

Query: 68  YFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVANSSYAKL 127
           +FCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ ANSSYAKL
Sbjct: 69  FFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQAANSSYAKL 128

Query: 128 LNQSNGSMGLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGDQNMVCWGF 187
           LNQSNG MGLFDTH+NYHNMYSG GNGSSGEVEH STETATAMKQELLPNGDQNMV WGF
Sbjct: 129 LNQSNGRMGLFDTHNNYHNMYSGLGNGSSGEVEHASTETATAMKQELLPNGDQNMVSWGF 188

Query: 188 PWQFNNGDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230
           PWQFNN DGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM
Sbjct: 189 PWQFNNEDGNYMGDLDSGRESWSFNGNGIASSWHGLLNSPLM 230

BLAST of Carg20243 vs. ExPASy TrEMBL
Match: A0A6J1CYM4 (dof zinc finger protein DOF3.2-like OS=Momordica charantia OX=3673 GN=LOC111015391 PE=4 SV=1)

HSP 1 Score: 380.6 bits (976), Expect = 4.7e-102
Identity = 201/264 (76.14%), Postives = 213/264 (80.68%), Query Frame = 0

Query: 1   MDHSSGQ-QEAQARPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYN 60
           MDHSSGQ  EAQA+PLE+M+  ISCPKVQQENRKPMRPQPEQ LRCPRCDSTNTKFCYYN
Sbjct: 1   MDHSSGQHHEAQAQPLENML-AISCPKVQQENRKPMRPQPEQALRCPRCDSTNTKFCYYN 60

Query: 61  NYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQ- 120
           NYSLSQPRYFCKSCRRYWTKGGTLRNVPVGG CRKNKRPSPSTSSSS  SPSSKRSHDQ 
Sbjct: 61  NYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRKNKRPSPSTSSSS--SPSSKRSHDQP 120

Query: 121 -VANSS----------------------YAKLLNQSNGSMGLFDTHSNYHNMYSGFGNGS 180
             ANSS                      +AKL NQSNG MGLFDTH+N+HNMY G+G+GS
Sbjct: 121 PSANSSHLMLTDPHLPSLAFETTDLSLAFAKLQNQSNGQMGLFDTHNNFHNMYCGYGSGS 180

Query: 181 SGEVEHG----------STETATAMKQELLPNGDQNMVCWGFPWQFNNGDGNYMGDLDSG 230
           SGEVE+G          S   +TAMKQEL+ NGDQNMV WGFPWQFNNGDGNYMGDLDSG
Sbjct: 181 SGEVENGGLSNFEHYNSSGPASTAMKQELVSNGDQNMVLWGFPWQFNNGDGNYMGDLDSG 240

BLAST of Carg20243 vs. TAIR 10
Match: AT3G45610.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 153.3 bits (386), Expect = 2.4e-37
Identity = 107/247 (43.32%), Postives = 135/247 (54.66%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQEN-RKPM---RP-QPEQVLRCPRCDSTNTKF 60
           MD+SS  Q        +++GV SC     +N +KP+   RP  PEQ LRCPRCDSTNTKF
Sbjct: 1   MDYSSMHQ--------NVMGVSSCSTQDYQNQKKPLSATRPAPPEQSLRCPRCDSTNTKF 60

Query: 61  CYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSS-SSSPSPSSKR 120
           CYYNNYSLSQPRYFCKSCRRYWTKGG LRN+P+GG+ RK+KR S +T S  ++P P+   
Sbjct: 61  CYYNNYSLSQPRYFCKSCRRYWTKGGILRNIPIGGAYRKHKRSSSATKSLRTTPEPTMTH 120

Query: 121 SHDQVANSSYAKLLNQ-SNGSMGLFDTHSNYH------NMYSGFGNGSS--------GEV 180
                  +S+    N  SN  M L   ++  +      + + GFG+  S        G  
Sbjct: 121 DGKSFPTASFGYNNNNISNEQMELGLAYALLNKQPLGVSSHLGFGSSQSPMAMDGVYGTT 180

Query: 181 EHGSTETATAM-----KQELLPNGDQNMVCWGFPWQFNNGDGN-----YMGDLDSGRESW 217
            H    T  A        E +   D N V WGFPWQ N G G+     ++  +DSGRE W
Sbjct: 181 SHQMENTGYAFGNGGGGMEQMATSDPNRVLWGFPWQMNMGGGSGHGHGHVDQIDSGREIW 239

BLAST of Carg20243 vs. TAIR 10
Match: AT5G60200.1 (TARGET OF MONOPTEROS 6 )

HSP 1 Score: 150.2 bits (378), Expect = 2.0e-36
Identity = 98/220 (44.55%), Postives = 111/220 (50.45%), Query Frame = 0

Query: 36  RPQ-PEQVLRCPRCDSTNTKFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRK 95
           RPQ PE  LRCPRCDSTNTKFCYYNNYSL+QPRYFCKSCRRYWTKGGTLRN+PVGG CRK
Sbjct: 47  RPQPPELALRCPRCDSTNTKFCYYNNYSLTQPRYFCKSCRRYWTKGGTLRNIPVGGGCRK 106

Query: 96  NKRPSPSTSSSSSPSPSSKRSHDQVANS-----------------SYAKLLNQSNGSM-- 155
           NKR + S + S   +P    SHD    S                 ++A L  Q  GS   
Sbjct: 107 NKRSTSSAARSLRTTP-EPASHDGKVFSAAGFNGYSNNEHIDLSLAFALLNKQHPGSSSQ 166

Query: 156 ----------------GLFDTHSNYHNMYSGFGNGSSGEVEHGSTETATAMKQELLPNGD 211
                           G+F T     N    FGNGSSG                    GD
Sbjct: 167 LGFHSELGSSHQSDMEGMFGTSQQKENATYAFGNGSSG-------------------LGD 226

BLAST of Carg20243 vs. TAIR 10
Match: AT2G28510.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 147.1 bits (370), Expect = 1.7e-35
Identity = 107/291 (36.77%), Postives = 136/291 (46.74%), Query Frame = 0

Query: 7   QQEAQARPLEDMVGVISCPKVQQENRKPMRPQPE----------QVLRCPRCDSTNTKFC 66
           +QE     LE +  ++S  K    N K M  + +          +   CPRC+S NTKFC
Sbjct: 4   EQEISNETLETI--LVSSTKGSNNNNKKMEEEMKKKVSRGELGGEAQNCPRCESPNTKFC 63

Query: 67  YYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSS--------- 126
           YYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGG CR+NKR S S  S ++         
Sbjct: 64  YYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGGCRRNKRSSSSAFSKNNNNKSINFHT 123

Query: 127 -----------PSPSSKRSHDQVANSSYAKL----------------------LNQSNGS 186
                      P  S    H    N ++A L                         S   
Sbjct: 124 DPLQNPLITGMPPSSFGYDHSIDLNLAFATLQKHHLSSQATTPSFGFGGDLSIYGNSTND 183

Query: 187 MGLFDTHSNYHN------MYSGFGNGSSGEVEHGST----ETATAMKQELLPNGDQN--- 230
           +G+F   +  +N        SG GN +  E++  ST          KQE + N + N   
Sbjct: 184 VGIFGGQNGTYNNSLCYGFMSGNGNNNQNEIKMASTLGMSLEGNERKQENVNNNNNNSEN 243

BLAST of Carg20243 vs. TAIR 10
Match: AT5G62940.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 132.1 bits (331), Expect = 5.6e-31
Identity = 79/156 (50.64%), Postives = 97/156 (62.18%), Query Frame = 0

Query: 13  RPLEDMVGVISCPKVQQENRKPMRPQPEQVLRCPRCDSTNTKFCYYNNYSLSQPRYFCKS 72
           RP    V V +   +    R  +RP  +   +CPRC+ST+TKFCYYNNYSLSQPRYFCK+
Sbjct: 45  RPQASAVAVAAAALMDGGRR--LRPPHDHPQKCPRCESTHTKFCYYNNYSLSQPRYFCKT 104

Query: 73  CRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSKRSHDQVANSSYAKLLNQSN 132
           CRRYWTKGGTLRN+PVGG CRKNK+PS S++SSSS S   K S+   AN+S         
Sbjct: 105 CRRYWTKGGTLRNIPVGGGCRKNKKPS-SSNSSSSTSSGKKPSNIVTANTS--------- 164

Query: 133 GSMGLFDTHSNYHNMYSGFGN-----GSSGEVEHGS 164
             M L  +H NY +   GF +     GS    EHG+
Sbjct: 165 DLMALAHSHQNYQHSPLGFSHFGGMMGSYSTPEHGN 188

BLAST of Carg20243 vs. TAIR 10
Match: AT1G64620.1 (Dof-type zinc finger DNA-binding family protein )

HSP 1 Score: 127.1 bits (318), Expect = 1.8e-29
Identity = 71/130 (54.62%), Postives = 85/130 (65.38%), Query Frame = 0

Query: 1   MDHSSGQQEAQARPLEDMVGVISCPKVQQENRKP-------MRPQPEQVLRCPRCDSTNT 60
           MD +   QE   +P+ ++V   +C K Q     P        RP+ +Q L CPRC+S NT
Sbjct: 1   MDTAKWPQEFVVKPMNEIV-TNTCLKQQSNPPSPATPVERKARPEKDQALNCPRCNSLNT 60

Query: 61  KFCYYNNYSLSQPRYFCKSCRRYWTKGGTLRNVPVGGSCRKNKRPSPSTSSSSSPSPSSK 120
           KFCYYNNYSL+QPRYFCK CRRYWT GG+LRN+PVGG  RKNKR S S SSSSSPS SS 
Sbjct: 61  KFCYYNNYSLTQPRYFCKDCRRYWTAGGSLRNIPVGGGVRKNKR-SSSNSSSSSPSSSSS 120

Query: 121 RSHDQVANSS 124
                 AN++
Sbjct: 121 SKKPLFANNN 128

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7035454.11.1e-129100.00Dof zinc finger protein DOF3.2 [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022946981.14.4e-12697.82dof zinc finger protein DOF3.2-like isoform X2 [Cucurbita moschata][more]
XP_023532294.11.2e-12395.63dof zinc finger protein DOF3.2-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023007417.11.0e-12295.20dof zinc finger protein DOF2.1-like isoform X2 [Cucurbita maxima][more]
XP_022946980.11.1e-12197.75dof zinc finger protein DOF3.2-like isoform X1 [Cucurbita moschata][more]
Match NameE-valueIdentityDescription
Q9M1E63.3e-3643.32Dof zinc finger protein DOF3.2 OS=Arabidopsis thaliana OX=3702 GN=DOF3.2 PE=1 SV... [more]
Q84TE92.8e-3544.55Dof zinc finger protein DOF5.3 OS=Arabidopsis thaliana OX=3702 GN=DOF5.3 PE=2 SV... [more]
Q8LE432.4e-3436.77Dof zinc finger protein DOF2.1 OS=Arabidopsis thaliana OX=3702 GN=DOF2.1 PE=2 SV... [more]
Q9FM037.9e-3050.64Dof zinc finger protein DOF5.6 OS=Arabidopsis thaliana OX=3702 GN=DOF5.6 PE=2 SV... [more]
Q84JQ82.6e-2854.62Dof zinc finger protein DOF1.8 OS=Arabidopsis thaliana OX=3702 GN=DOF1.8 PE=2 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1G5482.1e-12697.82dof zinc finger protein DOF3.2-like isoform X2 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1KYM54.9e-12395.20dof zinc finger protein DOF2.1-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1G5J25.4e-12297.75dof zinc finger protein DOF3.2-like isoform X1 OS=Cucurbita moschata OX=3662 GN=... [more]
A0A6J1L0G71.2e-11895.05dof zinc finger protein DOF2.1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LO... [more]
A0A6J1CYM44.7e-10276.14dof zinc finger protein DOF3.2-like OS=Momordica charantia OX=3673 GN=LOC1110153... [more]
Match NameE-valueIdentityDescription
AT3G45610.12.4e-3743.32Dof-type zinc finger DNA-binding family protein [more]
AT5G60200.12.0e-3644.55TARGET OF MONOPTEROS 6 [more]
AT2G28510.11.7e-3536.77Dof-type zinc finger DNA-binding family protein [more]
AT5G62940.15.6e-3150.64Dof-type zinc finger DNA-binding family protein [more]
AT1G64620.11.8e-2954.62Dof-type zinc finger DNA-binding family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003851Zinc finger, Dof-typePFAMPF02701zf-Dofcoord: 41..97
e-value: 9.2E-34
score: 115.4
IPR003851Zinc finger, Dof-typePROSITEPS01361ZF_DOF_1coord: 45..81
IPR003851Zinc finger, Dof-typePROSITEPS50884ZF_DOF_2coord: 43..97
score: 29.31056
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 95..121
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 87..121
NoneNo IPR availablePANTHERPTHR31992DOF ZINC FINGER PROTEIN DOF1.4-RELATEDcoord: 7..229
NoneNo IPR availablePANTHERPTHR31992:SF221DOF ZINC FINGER PROTEIN DOF3.2-LIKEcoord: 7..229

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20243-RACarg20243-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006355 regulation of transcription, DNA-templated
cellular_component GO:0005634 nucleus
molecular_function GO:0003677 DNA binding