Carg20196 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg20196
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionElongation factor Tu
LocationCarg_Chr11: 326497 .. 327921 (+)
RNA-Seq ExpressionCarg20196
SyntenyCarg20196
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGCCATTTCCTTAGCTTCCGCTTCCACTTCCACTTCCTCCAAGCTCGCTTTCCCGCATCCCTCTCCTTCGCCCTTTTCCTCCAAACCCTCCTCTATCACCAACCTTTCTTCCTCTTTCTTGAACCCTTCTTCTCTTCGCCCTCTTACCTTCACTTCTTCCTCTTCCGCCACTGTTAAACGCTCTCCTTCCTTCACTATTCGCGCCGCTCGTGGCAAATTCGAGAGGAAGAAACCCCATGTCAATATCGGCACAATTGGGCACGTCGACCATGGTAAGACCACTCTCACCGCCGCTTTAACCATGGCTTTGAGCAAAGCCCCAAAGAAATATGACGAAATTGACGCCGCACCTGAAGAGCGAGCTCGGGGTATTACGATTAACACCGCCACCGTTGAGTACGAGACTGAGAGCCGACATTACGCCCACGTTGATTGCCCTGGCCATGCTGATTACGTCAAGAACATGATTACTGGTGCTGCTCAAATGGATGGCGCAATTTTGGTTGTTTCGGGCGCTGATGGCCCAATGCCACAAACCAAGGAGCATATTTTGTTGGCTAAGCAAGTGGGTGTCCCCAATATGGTGGTGTTTCTCAACAAGAAGGATCAAGTTGATGACGAGGAGCTTCTGGAGCTTGTGGAATTGGAGATGCGTGAATTGCTTTCGTCTTATGAATTTCCAGGTGATGATGTCCCAATTGTTGCTGGTTCTGCTTTGTTAGCTCTGGAAGCTCTAATGGCTAACCCCAATATTGTTAGAGGCGAAAATGAATGGGTGGATAAAATTTTTGAACTTATGGATTCTGTTGATAGCTATATTCCCATACCCGAAAGACAAACAGATCTTCCATTTTTGCTTGCTGTTGAAGATGTTTTCTCTATTACTGGTCGTGGTACCGTGGCGACAGGGCGTGTCGAGAGGGGTACCGTTAGAGTCGGAGAGACGGTGGATATTGTGGGATTGAGGGAAACTAGAAACACAACAGTAACAGGGGTGGAGATGTTTCAGAAAATTCTTGATGAAGCTTTGGCTGGGGATAATGTAGGATTGTTGCTTAGAGGTGTTCAAAAGGCTGATATCCAGAGAGGGATGGTTTTGGCCAAGCCTGGCACCATCACCCCACACACCAAGTTTGTTGCAGTTGTGTATGTGTTGAAGAAGGAAGAGGGTGGTAGACATTCACCCTTTTTCGCCGGTTACCGACCACAATTTTACATGAGGACGACCGATGTCACCGGTAAGGTGACTTCGATTATGAACGACAAGGACGAGGAGTCCAAAATGGTTATGCCAGGAGACCGAGTGAAAATGGTTGTTGAACTGATAATGCCAGTGGCTTGTGAACAAGGAATGAGGTTTGCCATCAGAGAAGGTGGAAAGACTGTTGGAGCTGGTGTGATCCAATCTATAATCGAGTGA

mRNA sequence

ATGGCGGCCATTTCCTTAGCTTCCGCTTCCACTTCCACTTCCTCCAAGCTCGCTTTCCCGCATCCCTCTCCTTCGCCCTTTTCCTCCAAACCCTCCTCTATCACCAACCTTTCTTCCTCTTTCTTGAACCCTTCTTCTCTTCGCCCTCTTACCTTCACTTCTTCCTCTTCCGCCACTGTTAAACGCTCTCCTTCCTTCACTATTCGCGCCGCTCGTGGCAAATTCGAGAGGAAGAAACCCCATGTCAATATCGGCACAATTGGGCACGTCGACCATGGTAAGACCACTCTCACCGCCGCTTTAACCATGGCTTTGAGCAAAGCCCCAAAGAAATATGACGAAATTGACGCCGCACCTGAAGAGCGAGCTCGGGGTATTACGATTAACACCGCCACCGTTGAGTACGAGACTGAGAGCCGACATTACGCCCACGTTGATTGCCCTGGCCATGCTGATTACGTCAAGAACATGATTACTGGTGCTGCTCAAATGGATGGCGCAATTTTGGTTGTTTCGGGCGCTGATGGCCCAATGCCACAAACCAAGGAGCATATTTTGTTGGCTAAGCAAGTGGGTGTCCCCAATATGGTGGTGTTTCTCAACAAGAAGGATCAAGTTGATGACGAGGAGCTTCTGGAGCTTGTGGAATTGGAGATGCGTGAATTGCTTTCGTCTTATGAATTTCCAGGTGATGATGTCCCAATTGTTGCTGGTTCTGCTTTGTTAGCTCTGGAAGCTCTAATGGCTAACCCCAATATTGTTAGAGGCGAAAATGAATGGGTGGATAAAATTTTTGAACTTATGGATTCTGTTGATAGCTATATTCCCATACCCGAAAGACAAACAGATCTTCCATTTTTGCTTGCTGTTGAAGATGTTTTCTCTATTACTGGTCGTGGTACCGTGGCGACAGGGCGTGTCGAGAGGGGTACCGTTAGAGTCGGAGAGACGGTGGATATTGTGGGATTGAGGGAAACTAGAAACACAACAGTAACAGGGGTGGAGATGTTTCAGAAAATTCTTGATGAAGCTTTGGCTGGGGATAATGTAGGATTGTTGCTTAGAGGTGTTCAAAAGGCTGATATCCAGAGAGGGATGGTTTTGGCCAAGCCTGGCACCATCACCCCACACACCAAGTTTGTTGCAGTTGTGTATGTGTTGAAGAAGGAAGAGGGTGGTAGACATTCACCCTTTTTCGCCGGTTACCGACCACAATTTTACATGAGGACGACCGATGTCACCGGTAAGGTGACTTCGATTATGAACGACAAGGACGAGGAGTCCAAAATGGTTATGCCAGGAGACCGAGTGAAAATGGTTGTTGAACTGATAATGCCAGTGGCTTGTGAACAAGGAATGAGGTTTGCCATCAGAGAAGGTGGAAAGACTGTTGGAGCTGGTGTGATCCAATCTATAATCGAGTGA

Coding sequence (CDS)

ATGGCGGCCATTTCCTTAGCTTCCGCTTCCACTTCCACTTCCTCCAAGCTCGCTTTCCCGCATCCCTCTCCTTCGCCCTTTTCCTCCAAACCCTCCTCTATCACCAACCTTTCTTCCTCTTTCTTGAACCCTTCTTCTCTTCGCCCTCTTACCTTCACTTCTTCCTCTTCCGCCACTGTTAAACGCTCTCCTTCCTTCACTATTCGCGCCGCTCGTGGCAAATTCGAGAGGAAGAAACCCCATGTCAATATCGGCACAATTGGGCACGTCGACCATGGTAAGACCACTCTCACCGCCGCTTTAACCATGGCTTTGAGCAAAGCCCCAAAGAAATATGACGAAATTGACGCCGCACCTGAAGAGCGAGCTCGGGGTATTACGATTAACACCGCCACCGTTGAGTACGAGACTGAGAGCCGACATTACGCCCACGTTGATTGCCCTGGCCATGCTGATTACGTCAAGAACATGATTACTGGTGCTGCTCAAATGGATGGCGCAATTTTGGTTGTTTCGGGCGCTGATGGCCCAATGCCACAAACCAAGGAGCATATTTTGTTGGCTAAGCAAGTGGGTGTCCCCAATATGGTGGTGTTTCTCAACAAGAAGGATCAAGTTGATGACGAGGAGCTTCTGGAGCTTGTGGAATTGGAGATGCGTGAATTGCTTTCGTCTTATGAATTTCCAGGTGATGATGTCCCAATTGTTGCTGGTTCTGCTTTGTTAGCTCTGGAAGCTCTAATGGCTAACCCCAATATTGTTAGAGGCGAAAATGAATGGGTGGATAAAATTTTTGAACTTATGGATTCTGTTGATAGCTATATTCCCATACCCGAAAGACAAACAGATCTTCCATTTTTGCTTGCTGTTGAAGATGTTTTCTCTATTACTGGTCGTGGTACCGTGGCGACAGGGCGTGTCGAGAGGGGTACCGTTAGAGTCGGAGAGACGGTGGATATTGTGGGATTGAGGGAAACTAGAAACACAACAGTAACAGGGGTGGAGATGTTTCAGAAAATTCTTGATGAAGCTTTGGCTGGGGATAATGTAGGATTGTTGCTTAGAGGTGTTCAAAAGGCTGATATCCAGAGAGGGATGGTTTTGGCCAAGCCTGGCACCATCACCCCACACACCAAGTTTGTTGCAGTTGTGTATGTGTTGAAGAAGGAAGAGGGTGGTAGACATTCACCCTTTTTCGCCGGTTACCGACCACAATTTTACATGAGGACGACCGATGTCACCGGTAAGGTGACTTCGATTATGAACGACAAGGACGAGGAGTCCAAAATGGTTATGCCAGGAGACCGAGTGAAAATGGTTGTTGAACTGATAATGCCAGTGGCTTGTGAACAAGGAATGAGGTTTGCCATCAGAGAAGGTGGAAAGACTGTTGGAGCTGGTGTGATCCAATCTATAATCGAGTGA

Protein sequence

MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Homology
BLAST of Carg20196 vs. NCBI nr
Match: XP_022933691.1 (elongation factor Tu, chloroplastic-like [Cucurbita moschata] >KAG7021422.1 Elongation factor Tu, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 910.6 bits (2352), Expect = 5.6e-261
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60
           MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV
Sbjct: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60

Query: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120
           KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE
Sbjct: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120

Query: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180
           ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ
Sbjct: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180

Query: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240
           TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA
Sbjct: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240

Query: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300
           LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG
Sbjct: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300

Query: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360
           TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA
Sbjct: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360

Query: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420
           DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI
Sbjct: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420

Query: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 474

BLAST of Carg20196 vs. NCBI nr
Match: KAG6587440.1 (Elongation factor Tu, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 909.1 bits (2348), Expect = 1.6e-260
Identity = 473/474 (99.79%), Postives = 473/474 (99.79%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60
           MAAISLASASTSTSSKL FPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV
Sbjct: 1   MAAISLASASTSTSSKLVFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60

Query: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120
           KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE
Sbjct: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120

Query: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180
           ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ
Sbjct: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180

Query: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240
           TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA
Sbjct: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240

Query: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300
           LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG
Sbjct: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300

Query: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360
           TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA
Sbjct: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360

Query: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420
           DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI
Sbjct: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420

Query: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 474

BLAST of Carg20196 vs. NCBI nr
Match: XP_023004504.1 (elongation factor Tu, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 905.6 bits (2339), Expect = 1.8e-259
Identity = 470/474 (99.16%), Postives = 473/474 (99.79%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60
           MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSIT LSSSFLNPSSLRPLTFTSSSS+TV
Sbjct: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITKLSSSFLNPSSLRPLTFTSSSSSTV 60

Query: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120
           KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE
Sbjct: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120

Query: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180
           ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ
Sbjct: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180

Query: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240
           TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA
Sbjct: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240

Query: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300
           LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG
Sbjct: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300

Query: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360
           TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA
Sbjct: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360

Query: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420
           DIQRGMVLAKPGTITPHTKF+AVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI
Sbjct: 361 DIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420

Query: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           MNDKDEESKMVMPGDRVKM+VELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 MNDKDEESKMVMPGDRVKMIVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 474

BLAST of Carg20196 vs. NCBI nr
Match: XP_023530750.1 (elongation factor Tu, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 904.8 bits (2337), Expect = 3.1e-259
Identity = 470/474 (99.16%), Postives = 472/474 (99.58%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60
           MAAISLASA TSTSSKLAFPHPSPSPFSSKPSSIT LSSSFLNPSSLRPLTFTSSSSATV
Sbjct: 1   MAAISLASAPTSTSSKLAFPHPSPSPFSSKPSSITKLSSSFLNPSSLRPLTFTSSSSATV 60

Query: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120
           KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE
Sbjct: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120

Query: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180
           ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ
Sbjct: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180

Query: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240
           TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPI+AGSA
Sbjct: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIIAGSA 240

Query: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300
           LLALEALMANPNIVRGENEWVDKIFELMD+VDSYIPIPERQTDLPFLLAVEDVFSITGRG
Sbjct: 241 LLALEALMANPNIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300

Query: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360
           TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA
Sbjct: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360

Query: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420
           DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI
Sbjct: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420

Query: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 474

BLAST of Carg20196 vs. NCBI nr
Match: XP_022135255.1 (elongation factor Tu, chloroplastic [Momordica charantia])

HSP 1 Score: 858.6 bits (2217), Expect = 2.5e-245
Identity = 450/480 (93.75%), Postives = 461/480 (96.04%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFS------SKPSSITNLSSSFLNPSSLRPLTFTS 60
           MAAISLASASTST  KL FPH SPSP S      +KPSSI  LSSSFLNPSS+RPLTF+S
Sbjct: 1   MAAISLASASTST--KLVFPHASPSPSSPPASLFAKPSSIPKLSSSFLNPSSIRPLTFSS 60

Query: 61  SSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120
            SSATV R  SFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE
Sbjct: 61  GSSATVARPRSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120

Query: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180
           IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240
           DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELL+LVELEMRELLSSYEFPGDDVP
Sbjct: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLQLVELEMRELLSSYEFPGDDVP 240

Query: 241 IVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVF 300
           I+AGSALLALEALMAN +I RGENEWVDKIFELMD+VDSYIPIPERQTDLPFLLAVEDVF
Sbjct: 241 IIAGSALLALEALMANQSIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300

Query: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360
           SITGRGTVATGRVERGT+RVG+TVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL
Sbjct: 301 SITGRGTVATGRVERGTIRVGDTVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 361 RGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420
           RGVQKADIQRGMVL+KPGTITPHTKF+AVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKADIQRGMVLSKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 421 GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 478

BLAST of Carg20196 vs. ExPASy Swiss-Prot
Match: P46280 (Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFB1 PE=3 SV=1)

HSP 1 Score: 773.5 bits (1996), Expect = 1.4e-222
Identity = 405/486 (83.33%), Postives = 447/486 (91.98%), Query Frame = 0

Query: 3   AISLASASTSTSSKLAFP---HPSPSPFSS-------KPSSITNLSSSFLNPSSLRPLTF 62
           AIS A+A   T+SKLA+P   H SPSP S+       KPS+ T+LSSSF++P+++  L  
Sbjct: 2   AISWAAA---TTSKLAYPPHVHFSPSPSSNYLFLKTHKPSA-THLSSSFIHPTTILHL-- 61

Query: 63  TSSSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL----SKA 122
             +++ T  R  SFT+RAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL    + A
Sbjct: 62  --AAANTTTRRRSFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASLGNSA 121

Query: 123 PKKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAI 182
           PKKYDEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAI
Sbjct: 122 PKKYDEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAI 181

Query: 183 LVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEF 242
           LVVSGADGPMPQTKEHILLAKQVGVPN+VVFLNK+DQVDDEELL+LVELE+RELLS YEF
Sbjct: 182 LVVSGADGPMPQTKEHILLAKQVGVPNIVVFLNKQDQVDDEELLQLVELEVRELLSKYEF 241

Query: 243 PGDDVPIVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLL 302
           PGDDVPI++GSALL+LEALMANP+I RGEN+WVDKI+ELM++VD YIPIP+RQT+LPFLL
Sbjct: 242 PGDDVPIISGSALLSLEALMANPSIKRGENQWVDKIYELMEAVDDYIPIPQRQTELPFLL 301

Query: 303 AVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGD 362
           A+EDVF+ITGRGTVATGRVERGT+RVGETVDIVG+++TRNTTVTGVEMFQKILDEALAGD
Sbjct: 302 AIEDVFTITGRGTVATGRVERGTIRVGETVDIVGVKDTRNTTVTGVEMFQKILDEALAGD 361

Query: 363 NVGLLLRGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYM 422
           NVGLLLRG+QK DIQRGMVLAKPGTITPHTKF A+VYVLKKEEGGRHSPFF+GYRPQFYM
Sbjct: 362 NVGLLLRGIQKTDIQRGMVLAKPGTITPHTKFSAIVYVLKKEEGGRHSPFFSGYRPQFYM 421

Query: 423 RTTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGV 475
           RTTDVTGKVT IMNDKDEESKMVMPGDRVK+VVELI+PVACEQGMRFAIREGGKTVGAGV
Sbjct: 422 RTTDVTGKVTEIMNDKDEESKMVMPGDRVKLVVELIVPVACEQGMRFAIREGGKTVGAGV 479

BLAST of Carg20196 vs. ExPASy Swiss-Prot
Match: Q43467 (Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFA PE=3 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 1.8e-222
Identity = 405/480 (84.38%), Postives = 440/480 (91.67%), Query Frame = 0

Query: 8   SASTSTSSKLAFPHPSPS------PFSSKPSS---ITNLSSSFLNPSSLRPLTFTSSSSA 67
           S++T++S  +  PH S S      PF S  ++   +T LSSSFL+P+++  L  T SS+ 
Sbjct: 4   SSATASSKLILLPHASSSSSLNSTPFRSSTTNTHKLTPLSSSFLHPTTV--LRRTPSSTT 63

Query: 68  TVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL----SKAPKKYDE 127
           T +R  +FT+RAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL    + APKKYDE
Sbjct: 64  TPRR--TFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALAALGNSAPKKYDE 123

Query: 128 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 187
           IDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 124 IDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 183

Query: 188 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 247
           DGPMPQTKEHI+LAKQVGVPNMVVFLNK+DQVDDEELL+LVE+E+R+LLSSYEFPGDD P
Sbjct: 184 DGPMPQTKEHIILAKQVGVPNMVVFLNKQDQVDDEELLQLVEIEVRDLLSSYEFPGDDTP 243

Query: 248 IVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVF 307
           IV+GSALLALEALMANP I RG+NEWVDKIF+LMD VD+YIPIP+RQTDLPFLLAVEDVF
Sbjct: 244 IVSGSALLALEALMANPAIKRGDNEWVDKIFQLMDEVDNYIPIPQRQTDLPFLLAVEDVF 303

Query: 308 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 367
           SITGRGTVATGRVERGT++VGETVD+VGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL
Sbjct: 304 SITGRGTVATGRVERGTIKVGETVDLVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 363

Query: 368 RGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 427
           RGVQK DIQRGMVLAKPGTITPHTKF A+VYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 364 RGVQKTDIQRGMVLAKPGTITPHTKFSAIVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 423

Query: 428 GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           GKVTSIMNDKDEES MV+PGDRVKMVVELI+PVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 424 GKVTSIMNDKDEESTMVLPGDRVKMVVELIVPVACEQGMRFAIREGGKTVGAGVIQSIIE 479

BLAST of Carg20196 vs. ExPASy Swiss-Prot
Match: Q43364 (Elongation factor TuB, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFB PE=2 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 7.2e-219
Identity = 404/487 (82.96%), Postives = 440/487 (90.35%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPH-PSPSPFSSKP-------SSITNLSSSFL-NPSSLRPLT 60
           MA+IS ASA+ + S+KLA+P+ PS S  SS         SS   LSSSF   PS+L   +
Sbjct: 1   MASISAASATATASTKLAYPYSPSSSSSSSNTAAVFPSNSSKLILSSSFTPTPSTLFLHS 60

Query: 61  FTSSSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL----SK 120
            T++ S T  R   FT+RAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL    + 
Sbjct: 61  PTTTPSTTHPR--RFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASMGNS 120

Query: 121 APKKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGA 180
           APKKYDEIDAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGA
Sbjct: 121 APKKYDEIDAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGA 180

Query: 181 ILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYE 240
           ILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNK+DQVDDEELLELVELE+RELLSSYE
Sbjct: 181 ILVVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLELVELEVRELLSSYE 240

Query: 241 FPGDDVPIVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFL 300
           FPGD++PI++GSALLALEALMANP+I RGEN+WVDKI++LMD+VD YIPIP+RQT+LPFL
Sbjct: 241 FPGDEIPIISGSALLALEALMANPSIKRGENQWVDKIYQLMDNVDEYIPIPQRQTELPFL 300

Query: 301 LAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAG 360
           +A+EDVFSITGRGTVATGRVERGTV+VGE VDIVGL++TRNTTVTGVEMFQKILDEA+AG
Sbjct: 301 MAIEDVFSITGRGTVATGRVERGTVKVGEIVDIVGLKDTRNTTVTGVEMFQKILDEAMAG 360

Query: 361 DNVGLLLRGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFY 420
           DNVGLLLRG+QK DIQRGMVLAKPGTITPHTKF A+VYVLKKEEGGRHSPFFAGYRPQFY
Sbjct: 361 DNVGLLLRGIQKIDIQRGMVLAKPGTITPHTKFEALVYVLKKEEGGRHSPFFAGYRPQFY 420

Query: 421 MRTTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAG 475
           MRTTDVTGKVT IM+DK EESKMVMPGDRV MVVELIMPVACEQGMRFAIREGGKTVGAG
Sbjct: 421 MRTTDVTGKVTVIMSDKGEESKMVMPGDRVNMVVELIMPVACEQGMRFAIREGGKTVGAG 480

BLAST of Carg20196 vs. ExPASy Swiss-Prot
Match: Q40450 (Elongation factor TuA, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFA PE=2 SV=2)

HSP 1 Score: 753.4 bits (1944), Expect = 1.5e-216
Identity = 396/479 (82.67%), Postives = 432/479 (90.19%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFL-NPSSLRPLTFTSSSSAT 60
           MA+IS A+A++ST    +       P S+KPS +  LSSSF  N S+L   +  + SS  
Sbjct: 1   MASISAATATSSTKLVSSNSTNPLLPSSTKPSKLI-LSSSFTPNCSTLFLHSPATPSSTA 60

Query: 61  VKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL----SKAPKKYDEI 120
             R   FT+RAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL    + APKKYDEI
Sbjct: 61  THRHRRFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASMGNSAPKKYDEI 120

Query: 121 DAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGAD 180
           DAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILV SGAD
Sbjct: 121 DAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSGAD 180

Query: 181 GPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPI 240
           GPMPQTKEHILLAKQVGVPNMVVFLNK+DQVDDEELL+LVELE+RELLSSYEFPGDD+PI
Sbjct: 181 GPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLQLVELEVRELLSSYEFPGDDIPI 240

Query: 241 VAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFS 300
           ++GSALLALEALMANP+I RGEN+WVDKI+ELMD+VDSYIPIP RQT+LPFL+A+EDVFS
Sbjct: 241 ISGSALLALEALMANPSIKRGENQWVDKIYELMDAVDSYIPIPVRQTELPFLMAIEDVFS 300

Query: 301 ITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLR 360
           ITGRGTVATGRVERGTVR+G+TVDIVGL++TR+TTVTGVEMFQKILDEA+AGDNVGLLLR
Sbjct: 301 ITGRGTVATGRVERGTVRIGDTVDIVGLKDTRSTTVTGVEMFQKILDEAMAGDNVGLLLR 360

Query: 361 GVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTG 420
           G+QK DIQRGMVLAKPGTITPHTKF A+VYVLKKEEGGRHSPFF+GYRPQFYMRTTDVTG
Sbjct: 361 GIQKIDIQRGMVLAKPGTITPHTKFEAIVYVLKKEEGGRHSPFFSGYRPQFYMRTTDVTG 420

Query: 421 KVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           KVTSI  DK EESKMVMPGDRV +VVELIMPVACEQGMRFAIREGGKTVGAGVIQ IIE
Sbjct: 421 KVTSITTDKGEESKMVMPGDRVNLVVELIMPVACEQGMRFAIREGGKTVGAGVIQKIIE 478

BLAST of Carg20196 vs. ExPASy Swiss-Prot
Match: P68158 (Elongation factor Tu, chloroplastic OS=Nicotiana tabacum OX=4097 GN=TUFA PE=3 SV=1)

HSP 1 Score: 753.4 bits (1944), Expect = 1.5e-216
Identity = 396/479 (82.67%), Postives = 432/479 (90.19%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFL-NPSSLRPLTFTSSSSAT 60
           MA+IS A+A++ST    +       P S+KPS +  LSSSF  N S+L   +  + SS  
Sbjct: 1   MASISAATATSSTKLVSSNSTNPLLPSSTKPSKLI-LSSSFTPNCSTLFLHSPATPSSTA 60

Query: 61  VKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL----SKAPKKYDEI 120
             R   FT+RAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL    + APKKYDEI
Sbjct: 61  THRHRRFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASMGNSAPKKYDEI 120

Query: 121 DAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGAD 180
           DAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILV SGAD
Sbjct: 121 DAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVCSGAD 180

Query: 181 GPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPI 240
           GPMPQTKEHILLAKQVGVPNMVVFLNK+DQVDDEELL+LVELE+RELLSSYEFPGDD+PI
Sbjct: 181 GPMPQTKEHILLAKQVGVPNMVVFLNKQDQVDDEELLQLVELEVRELLSSYEFPGDDIPI 240

Query: 241 VAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFS 300
           ++GSALLALEALMANP+I RGEN+WVDKI+ELMD+VDSYIPIP RQT+LPFL+A+EDVFS
Sbjct: 241 ISGSALLALEALMANPSIKRGENQWVDKIYELMDAVDSYIPIPVRQTELPFLMAIEDVFS 300

Query: 301 ITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLR 360
           ITGRGTVATGRVERGTVR+G+TVDIVGL++TR+TTVTGVEMFQKILDEA+AGDNVGLLLR
Sbjct: 301 ITGRGTVATGRVERGTVRIGDTVDIVGLKDTRSTTVTGVEMFQKILDEAMAGDNVGLLLR 360

Query: 361 GVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTG 420
           G+QK DIQRGMVLAKPGTITPHTKF A+VYVLKKEEGGRHSPFF+GYRPQFYMRTTDVTG
Sbjct: 361 GIQKIDIQRGMVLAKPGTITPHTKFEAIVYVLKKEEGGRHSPFFSGYRPQFYMRTTDVTG 420

Query: 421 KVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           KVTSI  DK EESKMVMPGDRV +VVELIMPVACEQGMRFAIREGGKTVGAGVIQ IIE
Sbjct: 421 KVTSITTDKGEESKMVMPGDRVNLVVELIMPVACEQGMRFAIREGGKTVGAGVIQKIIE 478

BLAST of Carg20196 vs. ExPASy TrEMBL
Match: A0A6J1EZS3 (Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111441027 PE=3 SV=1)

HSP 1 Score: 910.6 bits (2352), Expect = 2.7e-261
Identity = 474/474 (100.00%), Postives = 474/474 (100.00%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60
           MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV
Sbjct: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60

Query: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120
           KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE
Sbjct: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120

Query: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180
           ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ
Sbjct: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180

Query: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240
           TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA
Sbjct: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240

Query: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300
           LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG
Sbjct: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300

Query: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360
           TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA
Sbjct: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360

Query: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420
           DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI
Sbjct: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420

Query: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 474

BLAST of Carg20196 vs. ExPASy TrEMBL
Match: A0A6J1KWG0 (Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111497788 PE=3 SV=1)

HSP 1 Score: 905.6 bits (2339), Expect = 8.8e-260
Identity = 470/474 (99.16%), Postives = 473/474 (99.79%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITNLSSSFLNPSSLRPLTFTSSSSATV 60
           MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSIT LSSSFLNPSSLRPLTFTSSSS+TV
Sbjct: 1   MAAISLASASTSTSSKLAFPHPSPSPFSSKPSSITKLSSSFLNPSSLRPLTFTSSSSSTV 60

Query: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120
           KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE
Sbjct: 61  KRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDEIDAAPE 120

Query: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180
           ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ
Sbjct: 121 ERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQ 180

Query: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240
           TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA
Sbjct: 181 TKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSA 240

Query: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300
           LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG
Sbjct: 241 LLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRG 300

Query: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360
           TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA
Sbjct: 301 TVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKA 360

Query: 361 DIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420
           DIQRGMVLAKPGTITPHTKF+AVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI
Sbjct: 361 DIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSI 420

Query: 421 MNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           MNDKDEESKMVMPGDRVKM+VELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 MNDKDEESKMVMPGDRVKMIVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 474

BLAST of Carg20196 vs. ExPASy TrEMBL
Match: A0A6J1C0N2 (Elongation factor Tu OS=Momordica charantia OX=3673 GN=LOC111007263 PE=3 SV=1)

HSP 1 Score: 858.6 bits (2217), Expect = 1.2e-245
Identity = 450/480 (93.75%), Postives = 461/480 (96.04%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFS------SKPSSITNLSSSFLNPSSLRPLTFTS 60
           MAAISLASASTST  KL FPH SPSP S      +KPSSI  LSSSFLNPSS+RPLTF+S
Sbjct: 1   MAAISLASASTST--KLVFPHASPSPSSPPASLFAKPSSIPKLSSSFLNPSSIRPLTFSS 60

Query: 61  SSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120
            SSATV R  SFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE
Sbjct: 61  GSSATVARPRSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE 120

Query: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180
           IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA
Sbjct: 121 IDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGA 180

Query: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVP 240
           DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELL+LVELEMRELLSSYEFPGDDVP
Sbjct: 181 DGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLQLVELEMRELLSSYEFPGDDVP 240

Query: 241 IVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVF 300
           I+AGSALLALEALMAN +I RGENEWVDKIFELMD+VDSYIPIPERQTDLPFLLAVEDVF
Sbjct: 241 IIAGSALLALEALMANQSIARGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVEDVF 300

Query: 301 SITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360
           SITGRGTVATGRVERGT+RVG+TVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL
Sbjct: 301 SITGRGTVATGRVERGTIRVGDTVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLL 360

Query: 361 RGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420
           RGVQKADIQRGMVL+KPGTITPHTKF+AVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT
Sbjct: 361 RGVQKADIQRGMVLSKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVT 420

Query: 421 GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE
Sbjct: 421 GKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 478

BLAST of Carg20196 vs. ExPASy TrEMBL
Match: A0A6J1E4U0 (Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111429936 PE=3 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.2e-239
Identity = 449/485 (92.58%), Postives = 455/485 (93.81%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSS-------KPSSITN----LSSSFLNPSSLRP 60
           MAAISLASA  STSS L F H S SP SS       KPSS ++    LSSSFLNPSS+RP
Sbjct: 1   MAAISLASA--STSSNLLFLHASASPSSSPSSSLFVKPSSSSSSAIKLSSSFLNPSSIRP 60

Query: 61  LTFTSSSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAP 120
           LTF   SS  VK   S TIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAP
Sbjct: 61  LTF---SSPAVKPPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAP 120

Query: 121 KKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAIL 180
           KKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAIL
Sbjct: 121 KKYDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAIL 180

Query: 181 VVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFP 240
           VVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFP
Sbjct: 181 VVSGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFP 240

Query: 241 GDDVPIVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLA 300
           GDDVPI+AGSALLALEALMANP IVRGENEWVDKIFELMD+VDSYIPIPERQTDLPFLLA
Sbjct: 241 GDDVPIIAGSALLALEALMANPGIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLA 300

Query: 301 VEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDN 360
           VEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDN
Sbjct: 301 VEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDN 360

Query: 361 VGLLLRGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMR 420
           VGLLLRGVQKADIQRGMVLAKPGTITPHTKF+AVVYVLKKEEGGRHSPFFAGYRPQFYMR
Sbjct: 361 VGLLLRGVQKADIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMR 420

Query: 421 TTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVI 475
           TTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVI
Sbjct: 421 TTDVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVI 480

BLAST of Carg20196 vs. ExPASy TrEMBL
Match: A0A6J1JDL1 (Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111484851 PE=3 SV=1)

HSP 1 Score: 837.0 bits (2161), Expect = 3.8e-239
Identity = 448/483 (92.75%), Postives = 455/483 (94.20%), Query Frame = 0

Query: 1   MAAISLASASTSTSSKLAFPHPSPSPFSS-------KPSSIT--NLSSSFLNPSSLRPLT 60
           MAAISLASA  STSS L F H S SP SS       KPSS +   LSSSFLNPSS+RPLT
Sbjct: 1   MAAISLASA--STSSNLLFLHASASPSSSPSSSLFAKPSSSSAIKLSSSFLNPSSIRPLT 60

Query: 61  FTSSSSATVKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKK 120
           F+SS+   V    S TIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKK
Sbjct: 61  FSSSA---VNPPRSLTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKK 120

Query: 121 YDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVV 180
           YDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVV
Sbjct: 121 YDEIDAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVV 180

Query: 181 SGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGD 240
           SGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGD
Sbjct: 181 SGADGPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGD 240

Query: 241 DVPIVAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVE 300
           DVPI+AGSALLALEALMANP IVRGENEWVDKIFELMD+VDSYIPIPERQTDLPFLLAVE
Sbjct: 241 DVPIIAGSALLALEALMANPGIVRGENEWVDKIFELMDAVDSYIPIPERQTDLPFLLAVE 300

Query: 301 DVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG 360
           DVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG
Sbjct: 301 DVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVG 360

Query: 361 LLLRGVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTT 420
           LLLRGVQKADIQRGMVLAKPGTITPHTKF+AVVYVLKKEEGGRHSPFFAGYRPQFYMRTT
Sbjct: 361 LLLRGVQKADIQRGMVLAKPGTITPHTKFLAVVYVLKKEEGGRHSPFFAGYRPQFYMRTT 420

Query: 421 DVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQS 475
           DVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQS
Sbjct: 421 DVTGKVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQS 478

BLAST of Carg20196 vs. TAIR 10
Match: AT4G20360.1 (RAB GTPase homolog E1B )

HSP 1 Score: 743.4 bits (1918), Expect = 1.1e-214
Identity = 393/479 (82.05%), Postives = 430/479 (89.77%), Query Frame = 0

Query: 7   ASASTSTSSKLAFPHPSPSP-------FSSKPSSITNLSSSFLNPSSLRPLTFTSSSSAT 66
           A A+ S+SS++   + SPSP        S K  ++T LSSSFL   S   LT TS+S +T
Sbjct: 5   APAACSSSSRILCSYSSPSPSLCPAISTSGKLKTLT-LSSSFLPSYS---LTTTSASQST 64

Query: 67  VKRSPSFTIRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL----SKAPKKYDEI 126
            +   SFT+RAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMAL    S   KKYDEI
Sbjct: 65  RR---SFTVRAARGKFERKKPHVNIGTIGHVDHGKTTLTAALTMALASIGSSVAKKYDEI 124

Query: 127 DAAPEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGAD 186
           DAAPEERARGITINTATVEYETE+RHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGAD
Sbjct: 125 DAAPEERARGITINTATVEYETENRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGAD 184

Query: 187 GPMPQTKEHILLAKQVGVPNMVVFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPI 246
           GPMPQTKEHILLAKQVGVP+MVVFLNK+DQVDD ELLELVELE+RELLSSYEF GDD+PI
Sbjct: 185 GPMPQTKEHILLAKQVGVPDMVVFLNKEDQVDDAELLELVELEVRELLSSYEFNGDDIPI 244

Query: 247 VAGSALLALEALMANPNIVRGENEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFS 306
           ++GSALLA+E L  NP + RG+N+WVDKI+ELMD+VD YIPIP+RQT+LPFLLAVEDVFS
Sbjct: 245 ISGSALLAVETLTENPKVKRGDNKWVDKIYELMDAVDDYIPIPQRQTELPFLLAVEDVFS 304

Query: 307 ITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEALAGDNVGLLLR 366
           ITGRGTVATGRVERGTV+VGETVD+VGLRETR+ TVTGVEMFQKILDEALAGDNVGLLLR
Sbjct: 305 ITGRGTVATGRVERGTVKVGETVDLVGLRETRSYTVTGVEMFQKILDEALAGDNVGLLLR 364

Query: 367 GVQKADIQRGMVLAKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTG 426
           G+QKADIQRGMVLAKPG+ITPHTKF A++YVLKKEEGGRHSPFFAGYRPQFYMRTTDVTG
Sbjct: 365 GIQKADIQRGMVLAKPGSITPHTKFEAIIYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTG 424

Query: 427 KVTSIMNDKDEESKMVMPGDRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSIIE 475
           KVT IMNDKDEESKMVMPGDRVK+VVELI+PVACEQGMRFAIREGGKTVGAGVI +I+E
Sbjct: 425 KVTKIMNDKDEESKMVMPGDRVKIVVELIVPVACEQGMRFAIREGGKTVGAGVIGTILE 476

BLAST of Carg20196 vs. TAIR 10
Match: AT4G02930.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 516.5 bits (1329), Expect = 2.2e-146
Identity = 282/459 (61.44%), Postives = 332/459 (72.33%), Query Frame = 0

Query: 43  NPSSLRPLTFTS------SSSATVKRSPSFTIRA---------------ARGKFERKKPH 102
           NPSS R + F+S       +S T   S S +I                 +   F R KPH
Sbjct: 8   NPSSKRLVPFSSQIYSRCGASVTSSYSISHSIGGDDLSSSTFGTSSFWRSMATFTRNKPH 67

Query: 103 VNIGTIGHVDHGKTTLTAALTMALSKAPK----KYDEIDAAPEERARGITINTATVEYET 162
           VN+GTIGHVDHGKTTLTAA+T  L++  K     +DEID APEE+ RGITI TA VEYET
Sbjct: 68  VNVGTIGHVDHGKTTLTAAITKVLAEEGKAKAIAFDEIDKAPEEKKRGITIATAHVEYET 127

Query: 163 ESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGPMPQTKEHILLAKQVGVPNMV 222
             RHYAHVDCPGHADYVKNMITGAAQMDG ILVVSG DGPMPQTKEHILLA+QVGVP++V
Sbjct: 128 AKRHYAHVDCPGHADYVKNMITGAAQMDGGILVVSGPDGPMPQTKEHILLARQVGVPSLV 187

Query: 223 VFLNKKDQVDDEELLELVELEMRELLSSYEFPGDDVPIVAGSALLALEALMANPNIVRGE 282
            FLNK D VDD ELLELVE+E+RELLS Y+FPGDD+PI+ GSAL AL+    N  I R  
Sbjct: 188 CFLNKVDVVDDPELLELVEMELRELLSFYKFPGDDIPIIRGSALSALQG--TNDEIGR-- 247

Query: 283 NEWVDKIFELMDSVDSYIPIPERQTDLPFLLAVEDVFSITGRGTVATGRVERGTVRVGET 342
                 I +LMD+VD YIP P R  D PFL+ +EDVFSI GRGTVATGR+E+G ++VGE 
Sbjct: 248 ----QAILKLMDAVDEYIPDPVRVLDKPFLMPIEDVFSIQGRGTVATGRIEQGVIKVGEE 307

Query: 343 VDIVGLRE---TRNTTVTGVEMFQKILDEALAGDNVGLLLRGVQKADIQRGMVLAKPGTI 402
           V+I+GLRE      +TVTGVEMF+KILD   AGDNVGLLLRG+++ DIQRGMV+AKPG+ 
Sbjct: 308 VEILGLREGGVPLKSTVTGVEMFKKILDNGQAGDNVGLLLRGLKREDIQRGMVIAKPGSC 367

Query: 403 TPHTKFVAVVYVLKKEEGGRHSPFFAGYRPQFYMRTTDVTGKVTSIMNDKDEESKMVMPG 462
             + KF A +YVL K+EGGRH+ FF+ YRPQFY+RT D+TGKV     +  E  KMVMPG
Sbjct: 368 KTYKKFEAEIYVLTKDEGGRHTAFFSNYRPQFYLRTADITGKV-----ELPENVKMVMPG 427

Query: 463 DRVKMVVELIMPVACEQGMRFAIREGGKTVGAGVIQSII 474
           D V  V ELIMPV  E G RFA+REGG+TVGAGV+  ++
Sbjct: 428 DNVTAVFELIMPVPLETGQRFALREGGRTVGAGVVSKVM 453

BLAST of Carg20196 vs. TAIR 10
Match: AT1G07920.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-39
Identity = 132/443 (29.80%), Postives = 196/443 (44.24%), Query Frame = 0

Query: 77  RKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE-------------------IDA 136
           ++K H+NI  IGHVD GK+T T  L   L    K+  E                   +D 
Sbjct: 3   KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62

Query: 137 APEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGP 196
              ER RGITI+ A  ++ET   +   +D PGH D++KNMITG +Q D A+L++    G 
Sbjct: 63  LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122

Query: 197 MP-------QTKEHILLAKQVGVPNMVVFLNKKDQVD---DEELLELVELEMRELLSSYE 256
                    QT+EH LLA  +GV  M+   NK D       +   + +  E+   L    
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182

Query: 257 FPGDDVPIVAGSALLALEALMANPNIVRGENEWV--DKIFELMDSVDSYIPIPERQTDLP 316
           +  D +P V  S       +  + N+     +W     + E +D ++     P+R +D P
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNL-----DWYKGPTLLEALDQINE----PKRPSDKP 242

Query: 317 FLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEAL 376
             L ++DV+ I G GTV  GRVE G ++ G  V       T  T V  VEM  + L EAL
Sbjct: 243 LRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLT--TEVKSVEMHHESLLEAL 302

Query: 377 AGDNVGLLLRGVQKADIQRGMVL--AKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYR 436
            GDNVG  ++ V   D++RG V   +K         F + V ++             GY 
Sbjct: 303 PGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM-----NHPGQIGNGYA 362

Query: 437 PQFYMRTTDVTGKVTSIMNDKD--------EESKMVMPGDRVKMVVELIMPVACEQGM-- 473
           P     T+ +  K + I+   D        +E K +  GD   + +    P+  E     
Sbjct: 363 PVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEY 422

BLAST of Carg20196 vs. TAIR 10
Match: AT1G07930.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-39
Identity = 132/443 (29.80%), Postives = 196/443 (44.24%), Query Frame = 0

Query: 77  RKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE-------------------IDA 136
           ++K H+NI  IGHVD GK+T T  L   L    K+  E                   +D 
Sbjct: 3   KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62

Query: 137 APEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGP 196
              ER RGITI+ A  ++ET   +   +D PGH D++KNMITG +Q D A+L++    G 
Sbjct: 63  LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122

Query: 197 MP-------QTKEHILLAKQVGVPNMVVFLNKKDQVD---DEELLELVELEMRELLSSYE 256
                    QT+EH LLA  +GV  M+   NK D       +   + +  E+   L    
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182

Query: 257 FPGDDVPIVAGSALLALEALMANPNIVRGENEWV--DKIFELMDSVDSYIPIPERQTDLP 316
           +  D +P V  S       +  + N+     +W     + E +D ++     P+R +D P
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNL-----DWYKGPTLLEALDQINE----PKRPSDKP 242

Query: 317 FLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEAL 376
             L ++DV+ I G GTV  GRVE G ++ G  V       T  T V  VEM  + L EAL
Sbjct: 243 LRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLT--TEVKSVEMHHESLLEAL 302

Query: 377 AGDNVGLLLRGVQKADIQRGMVL--AKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYR 436
            GDNVG  ++ V   D++RG V   +K         F + V ++             GY 
Sbjct: 303 PGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM-----NHPGQIGNGYA 362

Query: 437 PQFYMRTTDVTGKVTSIMNDKD--------EESKMVMPGDRVKMVVELIMPVACEQGM-- 473
           P     T+ +  K + I+   D        +E K +  GD   + +    P+  E     
Sbjct: 363 PVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEY 422

BLAST of Carg20196 vs. TAIR 10
Match: AT1G07940.1 (GTP binding Elongation factor Tu family protein )

HSP 1 Score: 161.0 bits (406), Expect = 2.3e-39
Identity = 132/443 (29.80%), Postives = 196/443 (44.24%), Query Frame = 0

Query: 77  RKKPHVNIGTIGHVDHGKTTLTAALTMALSKAPKKYDE-------------------IDA 136
           ++K H+NI  IGHVD GK+T T  L   L    K+  E                   +D 
Sbjct: 3   KEKFHINIVVIGHVDSGKSTTTGHLIYKLGGIDKRVIERFEKEAAEMNKRSFKYAWVLDK 62

Query: 137 APEERARGITINTATVEYETESRHYAHVDCPGHADYVKNMITGAAQMDGAILVVSGADGP 196
              ER RGITI+ A  ++ET   +   +D PGH D++KNMITG +Q D A+L++    G 
Sbjct: 63  LKAERERGITIDIALWKFETTKYYCTVIDAPGHRDFIKNMITGTSQADCAVLIIDSTTGG 122

Query: 197 MP-------QTKEHILLAKQVGVPNMVVFLNKKDQVD---DEELLELVELEMRELLSSYE 256
                    QT+EH LLA  +GV  M+   NK D       +   + +  E+   L    
Sbjct: 123 FEAGISKDGQTREHALLAFTLGVKQMICCCNKMDATTPKYSKARYDEIIKEVSSYLKKVG 182

Query: 257 FPGDDVPIVAGSALLALEALMANPNIVRGENEWV--DKIFELMDSVDSYIPIPERQTDLP 316
           +  D +P V  S       +  + N+     +W     + E +D ++     P+R +D P
Sbjct: 183 YNPDKIPFVPISGFEGDNMIERSTNL-----DWYKGPTLLEALDQINE----PKRPSDKP 242

Query: 317 FLLAVEDVFSITGRGTVATGRVERGTVRVGETVDIVGLRETRNTTVTGVEMFQKILDEAL 376
             L ++DV+ I G GTV  GRVE G ++ G  V       T  T V  VEM  + L EAL
Sbjct: 243 LRLPLQDVYKIGGIGTVPVGRVETGMIKPGMVVTFAPTGLT--TEVKSVEMHHESLLEAL 302

Query: 377 AGDNVGLLLRGVQKADIQRGMVL--AKPGTITPHTKFVAVVYVLKKEEGGRHSPFFAGYR 436
            GDNVG  ++ V   D++RG V   +K         F + V ++             GY 
Sbjct: 303 PGDNVGFNVKNVAVKDLKRGYVASNSKDDPAKGAANFTSQVIIM-----NHPGQIGNGYA 362

Query: 437 PQFYMRTTDVTGKVTSIMNDKD--------EESKMVMPGDRVKMVVELIMPVACEQGM-- 473
           P     T+ +  K + I+   D        +E K +  GD   + +    P+  E     
Sbjct: 363 PVLDCHTSHIAVKFSEILTKIDRRSGKEIEKEPKFLKNGDAGMVKMTPTKPMVVETFSEY 422

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
XP_022933691.15.6e-261100.00elongation factor Tu, chloroplastic-like [Cucurbita moschata] >KAG7021422.1 Elon... [more]
KAG6587440.11.6e-26099.79Elongation factor Tu, chloroplastic, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_023004504.11.8e-25999.16elongation factor Tu, chloroplastic-like [Cucurbita maxima][more]
XP_023530750.13.1e-25999.16elongation factor Tu, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
XP_022135255.12.5e-24593.75elongation factor Tu, chloroplastic [Momordica charantia][more]
Match NameE-valueIdentityDescription
P462801.4e-22283.33Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFB1 PE=3 SV=1[more]
Q434671.8e-22284.38Elongation factor Tu, chloroplastic OS=Glycine max OX=3847 GN=TUFA PE=3 SV=1[more]
Q433647.2e-21982.96Elongation factor TuB, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFB PE=... [more]
Q404501.5e-21682.67Elongation factor TuA, chloroplastic OS=Nicotiana sylvestris OX=4096 GN=TUFA PE=... [more]
P681581.5e-21682.67Elongation factor Tu, chloroplastic OS=Nicotiana tabacum OX=4097 GN=TUFA PE=3 SV... [more]
Match NameE-valueIdentityDescription
A0A6J1EZS32.7e-261100.00Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111441027 PE=3 SV=1[more]
A0A6J1KWG08.8e-26099.16Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111497788 PE=3 SV=1[more]
A0A6J1C0N21.2e-24593.75Elongation factor Tu OS=Momordica charantia OX=3673 GN=LOC111007263 PE=3 SV=1[more]
A0A6J1E4U02.2e-23992.58Elongation factor Tu OS=Cucurbita moschata OX=3662 GN=LOC111429936 PE=3 SV=1[more]
A0A6J1JDL13.8e-23992.75Elongation factor Tu OS=Cucurbita maxima OX=3661 GN=LOC111484851 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G20360.11.1e-21482.05RAB GTPase homolog E1B [more]
AT4G02930.12.2e-14661.44GTP binding Elongation factor Tu family protein [more]
AT1G07920.12.3e-3929.80GTP binding Elongation factor Tu family protein [more]
AT1G07930.12.3e-3929.80GTP binding Elongation factor Tu family protein [more]
AT1G07940.12.3e-3929.80GTP binding Elongation factor Tu family protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000795Translational (tr)-type GTP-binding domainPRINTSPR00315ELONGATNFCTcoord: 83..96
score: 76.82
coord: 159..170
score: 66.17
coord: 196..205
score: 51.85
coord: 143..153
score: 63.79
coord: 123..131
score: 70.58
IPR000795Translational (tr)-type GTP-binding domainPFAMPF00009GTP_EFTUcoord: 79..276
e-value: 1.5E-54
score: 184.5
IPR000795Translational (tr)-type GTP-binding domainPROSITEPS51722G_TR_2coord: 79..279
score: 59.431015
IPR004160Translation elongation factor EFTu/EF1A, C-terminalPFAMPF03143GTP_EFTU_D3coord: 373..472
e-value: 1.1E-39
score: 135.0
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 71..278
e-value: 2.9E-71
score: 241.3
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 74..294
IPR004541Translation elongation factor EFTu/EF1A, bacterial/organelleTIGRFAMTIGR00485TIGR00485coord: 71..474
e-value: 1.7E-222
score: 736.2
IPR004541Translation elongation factor EFTu/EF1A, bacterial/organelleHAMAPMF_00118_BEF_Tu_Bcoord: 70..474
score: 58.952965
IPR005225Small GTP-binding protein domainTIGRFAMTIGR00231TIGR00231coord: 82..212
e-value: 1.3E-15
score: 55.5
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 413..473
e-value: 4.5E-23
score: 82.9
NoneNo IPR availableGENE3D2.40.30.10Translation factorscoord: 281..409
e-value: 2.5E-53
score: 181.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..34
NoneNo IPR availablePANTHERPTHR43721ELONGATION FACTOR TU-RELATEDcoord: 36..474
NoneNo IPR availablePANTHERPTHR43721:SF21ELONGATION FACTOR TUcoord: 36..474
NoneNo IPR availableCDDcd03707EFTU_IIIcoord: 375..469
e-value: 5.77712E-55
score: 176.548
IPR004161Translation elongation factor EFTu-like, domain 2PFAMPF03144GTP_EFTU_D2coord: 300..368
e-value: 2.0E-17
score: 63.3
IPR031157Tr-type G domain, conserved sitePROSITEPS00301G_TR_1coord: 116..131
IPR041709Elongation factor Tu (EF-Tu), GTP-binding domainCDDcd01884EF_Tucoord: 80..278
e-value: 5.22422E-118
score: 342.256
IPR033720Elongation factor Tu, domain 2CDDcd03697EFTU_IIcoord: 286..372
e-value: 2.5819E-50
score: 164.229
IPR009000Translation protein, beta-barrel domain superfamilySUPERFAMILY50447Translation proteinscoord: 280..376
IPR009001Translation elongation factor EF1A/initiation factor IF2gamma, C-terminalSUPERFAMILY50465EF-Tu/eEF-1alpha/eIF2-gamma C-terminal domaincoord: 373..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg20196-RACarg20196-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0070125 mitochondrial translational elongation
biological_process GO:0006414 translational elongation
cellular_component GO:0005739 mitochondrion
molecular_function GO:0003924 GTPase activity
molecular_function GO:0005525 GTP binding
molecular_function GO:0003746 translation elongation factor activity