Carg20193 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg20193
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Protein-L-isoaspartate O-methyltransferase
Location	Carg_Chr11: 308102 .. 311279 (+)
RNA-Seq Expression	Carg20193
Synteny	Carg20193

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

AGAGAGAGAGAGAGAGAGAGAGGGAGGCTAACTGGAAAAAACGAGAAGGGCGGAGAGAGAAATGAGTGTTCAATTATCTTTCCTCCCATTTGCTTATGGTTGCCGCTACTACATCACTCCCCTTCTCTCCACCGCCGATTTCCCACCGCCCTACCTCCGATCAATTCTTCTGCCCCTAAAACCACCGCCACCAGTATCGTCTCCGTCTTACCTCGACTTCGGTCTGCGAGTAATTGTCTACTTTCCAGGATGAAGGTACTCTCTCCTTCTCTCTCTCTCTCTCTCTCTCTCTATCTACCGCTAATTCCTTCCCACCCATTCTCTCTTCTTCTTTACAAAATTTACGTTTTCTGAAATTGGAGATTCTCGGATTTTCCCAAAACTGAAATTGGGATTCAAGGTATTTTGTGTTTGTATGTGCACGGACGACCCAATTCTTTTTATTTTTTGTATAGGATTTTCACCTAGGAGAAGGCTTTGATTATTATTAGCTGGTAATATATCGCCTCAACTGGAATTCGTAATTCATCTTTCTCTGTGCTCTGGATTTTTAATTGTTCTTTCAATCTGATGTATATGATCTTTTGCTTGAAAATTGTCGTTTAGTCCTCTCTTATGATACGAATTTGGTCTGTAGAGAACTAAATTTTCCCCTTGAAACTGCTTGATAATTTCATCGATGTTAGTTTTAATCATTCAAGGCATTTGTTTCCAATACATGTGGTTTTATTTCGAGTGGTTTTGGTAGTATATCTTATGCGGATATGTTTTCGTCGCTAACCCGCCCTGCTTCTGAGGCTGTGGAGCTTGATAAGGTACTTGCGTTGATGAGAATGAACTAAGGATCAGTTGGAAGATGGATGGATTTATAAATTAATGTTTAGAGATGGGGAGATAGCTTTAGGAACTCCTTGTGAATTGTGCTCTATTGGTGTGTTTCGGCGTGTTTCTGTTCAGGAATTCATTTTCAATTTTTGTTTCATGTCTTTGTTTTTGGTTGAAAGTTTGATCGTTCCATGTCATTTTAAATCACATTGCAGAGGTTCTGGAGTGGAAGTGGCATAGCTACAAACAAGGAAATGGTGAAGAATCTCCAGAACTATGGAGTGGTTGGGTCGAAGAAGGTGTCTGAAGTAATGGAGAGTATAGATAGGGCATTTTTTGTGCCGGATGATGCTCCACCTTATGTTGATACTCCCCTGCCAATTGGTTACAATGCCACTATATCTGCACCTCACATGCATGCAACCTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCGCGCTTTGGACGTTGGCTCAGGTTGTGCATCTAACTAAAAACTTCTATGCCCTGAAAATTGAATTGGTGATTTTTTTCATTGCTGTTGAATTCCTAAGAATGTTAAATTATGTTTGACTTGAGACATTCTGAATCTGGATTTCTAATCAGCAGTTTTCTTTGATCTGTAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAATCACTTTGGACATTTCGTTGAAGGTTAGCATTATCCCCCATACCGGTTGAAGAGGAGAACGAAGCATTCCTCATAAAGGTGTGGAAACCTCTTTCTAGTAGATGCGTTTTAAAAACCGTGAGGCTGACGGCGATACGTAACTGGCCAAAGCGGACAATATCTACTAGCGGTGGGCTTGGACTGTTACAAATGGTATCAAAGCCAAACACCGGGTGGTGTGCCAGCGAGGGCATTGGCCCCCAAGGGAGTGGATTGTGAGATCCGACATCGGTTGGAGAAGAGAATGAAGCATTTCTTATGAGGGTGAAAAGAAAAAGAAAAGAAAAGATAAGAAATTGGTATAATAGATGAAATTGAAATGCAATGATTCTGGTCTTTCCTGTAAGGGGTTCAGTGTGCTCTAAACATGCACTGCCCAAGTGAAAGTGCAAGTTGGTTACTCTAAAAAGATACAACTCTATTACCTTTTCGCATTTGAAATTTGCATTTTCAACTTCAAATGCCATTTCCTTGACCTTCCTCGGTCTTCCTATATATTTAAGTTGAATTTACATGTTTGATTTCTCCTGGGCAGGAACGGGATATTTGACTGCATGCTTCGCGTTAATGATTGGACCTGAAGGTCGTGCAGTTGGTGTGGAACATATTCCTGAGCTGGTGGCTTCATCAATCGAGAATATTAATAAAAGTGCTGCTGCTCCTTTACTGAAAGAAGGTTCCCTCTCTCTACACGTTGCTGGTATGTTTGCCTTCTTTCCCTTTTCTAGAGCCATAGATTTTTCTTTTGATTTAGAATAAAGAACTCTGTTCCTTAGTTTAATTGGCCGTAAAGAGATGAAGGATGTAGTGAAGTGACCTTTAAGCAATTTTCGTGCACCCGCTATGCACTGGAATGGTATATTCTTGAAAGTAAAAGGTAAGTTCGAGATATTTTTAAAAGTAGTGCTTTACCCTTTGAGCGGAACACTTAGAAAACAAAACACTGTAACTAGAGTTCTATTACTACAGAGTGCTTCGAATTTTTGTTTAAAATATCTCAAGCTACGTCTTGGGTAGATAACTTAAAGCCATTCTAAAAAATCCTTAAATTTAATTAAGAACTTCTTACTACTACGTAAGTCATGCTAAACCCGTCTAAAACGAGATTTCTCCCCTTTGAAACTAGATGAGGAGAAAATTTGAGCTCAATTCTCCTTTAGAAATCCATTAGTGTCAGTCACCTCCCTCCCCAGCGCTCACACTTCAATCTTAACAATGTTCTTCGGTGAACTGCTGTGGCTAATGAGGGTGAGAAGATCTAACATGGCAAAAGTAGAATGTGGGTATTAATCCGAGGGCGTGTTTGTCCTACTCGATGATGTGTGTTGTTAATGAAAGTTTGTGGTTACTTTGTTATTGGATGGTTGTAGATGGCAGGCAGGGCTGGCCAGAGTGTTCACCTTATGATGCTATTCATGTTGGGGCAGCAGCTGCTGAAATTCCGCCGGCTCTCATTGACCAGTTAAAGCCAGGTGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGATGGTTGTGGATAAAGATGCCGATGGCTCTGTTAACATCCACAACGAGACTTCTGTTCGTTATGTGCCTCTTACCAGCCGAGAAGCTCAGCTACGTGACAACTGA

mRNA sequence

AGAGAGAGAGAGAGAGAGAGAGGGAGGCTAACTGGAAAAAACGAGAAGGGCGGAGAGAGAAATGAGTGTTCAATTATCTTTCCTCCCATTTGCTTATGGTTGCCGCTACTACATCACTCCCCTTCTCTCCACCGCCGATTTCCCACCGCCCTACCTCCGATCAATTCTTCTGCCCCTAAAACCACCGCCACCAGTATCGTCTCCGTCTTACCTCGACTTCGGTCTGCGAGTAATTGTCTACTTTCCAGGATGAAGGCATTTGTTTCCAATACATGTGGTTTTATTTCGAGTGGTTTTGGTAGTATATCTTATGCGGATATGTTTTCGTCGCTAACCCGCCCTGCTTCTGAGGCTGTGGAGCTTGATAAGAGGTTCTGGAGTGGAAGTGGCATAGCTACAAACAAGGAAATGGTGAAGAATCTCCAGAACTATGGAGTGGTTGGGTCGAAGAAGGTGTCTGAAGTAATGGAGAGTATAGATAGGGCATTTTTTGTGCCGGATGATGCTCCACCTTATGTTGATACTCCCCTGCCAATTGGTTACAATGCCACTATATCTGCACCTCACATGCATGCAACCTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCGCGCTTTGGACGTTGGCTCAGGAACGGGATATTTGACTGCATGCTTCGCGTTAATGATTGGACCTGAAGGTCGTGCAGTTGGTGTGGAACATATTCCTGAGCTGGTGGCTTCATCAATCGAGAATATTAATAAAAGTGCTGCTGCTCCTTTACTGAAAGAAGGTTCCCTCTCTCTACACGTTGCTGATGGCAGGCAGGGCTGGCCAGAGTGTTCACCTTATGATGCTATTCATGTTGGGGCAGCAGCTGCTGAAATTCCGCCGGCTCTCATTGACCAGTTAAAGCCAGGTGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGATGGTTGTGGATAAAGATGCCGATGGCTCTGTTAACATCCACAACGAGACTTCTGTTCGTTATGTGCCTCTTACCAGCCGAGAAGCTCAGCTACGTGACAACTGA

Coding sequence (CDS)

AGAGAGAGAGAGAGAGAGAGAGGGAGGCTAACTGGAAAAAACGAGAAGGGCGGAGAGAGAAATGAGTGTTCAATTATCTTTCCTCCCATTTGCTTATGGTTGCCGCTACTACATCACTCCCCTTCTCTCCACCGCCGATTTCCCACCGCCCTACCTCCGATCAATTCTTCTGCCCCTAAAACCACCGCCACCAGTATCGTCTCCGTCTTACCTCGACTTCGGTCTGCGAGTAATTGTCTACTTTCCAGGATGAAGGCATTTGTTTCCAATACATGTGGTTTTATTTCGAGTGGTTTTGGTAGTATATCTTATGCGGATATGTTTTCGTCGCTAACCCGCCCTGCTTCTGAGGCTGTGGAGCTTGATAAGAGGTTCTGGAGTGGAAGTGGCATAGCTACAAACAAGGAAATGGTGAAGAATCTCCAGAACTATGGAGTGGTTGGGTCGAAGAAGGTGTCTGAAGTAATGGAGAGTATAGATAGGGCATTTTTTGTGCCGGATGATGCTCCACCTTATGTTGATACTCCCCTGCCAATTGGTTACAATGCCACTATATCTGCACCTCACATGCATGCAACCTGCCTTCAATTGTTGGAGAAACACTTGCAACCTGGAATGCGCGCTTTGGACGTTGGCTCAGGAACGGGATATTTGACTGCATGCTTCGCGTTAATGATTGGACCTGAAGGTCGTGCAGTTGGTGTGGAACATATTCCTGAGCTGGTGGCTTCATCAATCGAGAATATTAATAAAAGTGCTGCTGCTCCTTTACTGAAAGAAGGTTCCCTCTCTCTACACGTTGCTGATGGCAGGCAGGGCTGGCCAGAGTGTTCACCTTATGATGCTATTCATGTTGGGGCAGCAGCTGCTGAAATTCCGCCGGCTCTCATTGACCAGTTAAAGCCAGGTGGGAGAATGGTGATTCCAGTTGGGAACATATTCCAGGATTTGATGGTTGTGGATAAAGATGCCGATGGCTCTGTTAACATCCACAACGAGACTTCTGTTCGTTATGTGCCTCTTACCAGCCGAGAAGCTCAGCTACGTGACAACTGA

Protein sequence

RERERERGRLTGKNEKGGERNECSIIFPPICLWLPLLHHSPSLHRRFPTALPPINSSAPKTTATSIVSVLPRLRSASNCLLSRMKAFVSNTCGFISSGFGSISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN

Homology

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BLAST of Carg20193 vs. NCBI nr
Match: KAG7021419.1 (Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 700.7 bits (1807), Expect = 6.5e-198
Identity = 351/351 (100.00%), Postives = 351/351 (100.00%), Query Frame = 0


Query: 1   RERERERGRLTGKNEKGGERNECSIIFPPICLWLPLLHHSPSLHRRFPTALPPINSSAPK 60
           RERERERGRLTGKNEKGGERNECSIIFPPICLWLPLLHHSPSLHRRFPTALPPINSSAPK
Sbjct: 1   RERERERGRLTGKNEKGGERNECSIIFPPICLWLPLLHHSPSLHRRFPTALPPINSSAPK 60

Query: 61  TTATSIVSVLPRLRSASNCLLSRMKAFVSNTCGFISSGFGSISYADMFSSLTRPASEAVE 120
           TTATSIVSVLPRLRSASNCLLSRMKAFVSNTCGFISSGFGSISYADMFSSLTRPASEAVE
Sbjct: 61  TTATSIVSVLPRLRSASNCLLSRMKAFVSNTCGFISSGFGSISYADMFSSLTRPASEAVE 120

Query: 121 LDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIG 180
           LDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIG
Sbjct: 121 LDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIG 180

Query: 181 YNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPE 240
           YNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPE
Sbjct: 181 YNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPE 240

Query: 241 LVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQL 300
           LVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQL
Sbjct: 241 LVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQL 300

Query: 301 KPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 352
           KPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 301 KPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 351

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BLAST of Carg20193 vs. NCBI nr
Match: KAG6587436.1 (Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 536.2 bits (1380), Expect = 2.1e-148
Identity = 266/268 (99.25%), Postives = 268/268 (100.00%), Query Frame = 0


Query: 84  MKAFVSNTCGFISSGFGSISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQN 143
           ++AFVSNTCGFISSGFGSISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQN
Sbjct: 56  LRAFVSNTCGFISSGFGSISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQN 115

Query: 144 YGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQ 203
           YGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQ
Sbjct: 116 YGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQ 175

Query: 204 PGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSL 263
           PGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSL
Sbjct: 176 PGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSL 235

Query: 264 SLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKD 323
           SLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKD
Sbjct: 236 SLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKD 295

Query: 324 ADGSVNIHNETSVRYVPLTSREAQLRDN 352
           ADGSVNIHNETSVRYVPLTSREAQLRDN
Sbjct: 296 ADGSVNIHNETSVRYVPLTSREAQLRDN 323

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BLAST of Carg20193 vs. NCBI nr
Match: XP_038879001.1 (protein-L-isoaspartate O-methyltransferase 1-like isoform X3 [Benincasa hispida])

HSP 1 Score: 484.6 bits (1246), Expect = 7.4e-133
Identity = 256/361 (70.91%), Postives = 275/361 (76.18%), Query Frame = 0


Query: 23  CSIIFPPICLWLPLLHHSPSLHRRFPTALPPINSSAPKTTATSIVSVLPRLRS----ASN 82
           C    P + L+  L    P   RR PTALPP  SS PKTT T+I+S LPR         N
Sbjct: 17  CRYCAPSLGLYFNLRLRRPRPRRRLPTALPPTTSSPPKTTTTTIISFLPRFLGPHFVTGN 76

Query: 83  CLLSRMK----------------------------AFVSNTCGFISSGFGSISYADMFSS 142
           C LSRM+                                +TC  +  G G +      SS
Sbjct: 77  CHLSRMEFQGVLFRVVWLVYSILVCLPWDMQSFDYDIRRHTCPSVLDGSGQV------SS 136

Query: 143 LTRPASEAVELDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAP 202
           L  PASEAVEL KRFW+GSGI TNKEMV+ L NYGVV SKKV+EVMESIDRAFFVPDD P
Sbjct: 137 LNGPASEAVELHKRFWTGSGIGTNKEMVEKLHNYGVVRSKKVAEVMESIDRAFFVPDDIP 196

Query: 203 PYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEG 262
           PYVDTP+PIGYNATISAPHMHATCLQLLEKHL+PGMRALDVGSGTGYLTACFALM+GPEG
Sbjct: 197 PYVDTPVPIGYNATISAPHMHATCLQLLEKHLKPGMRALDVGSGTGYLTACFALMVGPEG 256

Query: 263 RAVGVEHIPELVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAA 322
           RAVGVEHIPELVASS+ENI KSAAAPLLKEGSLS+HV DGRQGWPEC+PYDAIHVGAAAA
Sbjct: 257 RAVGVEHIPELVASSMENIKKSAAAPLLKEGSLSVHVGDGRQGWPECAPYDAIHVGAAAA 316

Query: 323 EIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRD 352
           EIPPALIDQLKPGGRMVIPVGNIFQDL VVDKD+DGSVNIH+ETSVRYVPLTSREAQLRD
Sbjct: 317 EIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDSDGSVNIHDETSVRYVPLTSREAQLRD 371

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BLAST of Carg20193 vs. NCBI nr
Match: TYJ98490.1 (protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo var. makuwa])

HSP 1 Score: 483.8 bits (1244), Expect = 1.3e-132
Identity = 256/350 (73.14%), Postives = 274/350 (78.29%), Query Frame = 0


Query: 28  PPICLWLPLLHHSPSLHRRFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSR 87
           P + L+  L  H     RR PTALPP  SS PKTT T+I+S LPR         NCLLSR
Sbjct: 23  PSLGLFFNLSCHHRRRRRRLPTALPPTTSSVPKTTVTTIISFLPRFLDPHFLTGNCLLSR 82

Query: 88  MKA---------FVSNTCGFISSGFGSISY-------------ADMFSSLTRPASEAVEL 147
           M+           V +    +S    S  Y                FSSL  PASEAVEL
Sbjct: 83  MEFQRGLSRVVWLVYSIVVCLSRDMQSFDYDIRWHTYPSELDGRGQFSSLNGPASEAVEL 142

Query: 148 DKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGY 207
           DKRFW+GSGI TNK+MV+ LQ YGVV SKKVSEVME+IDRAFFVPDD PPYVDTP+PIGY
Sbjct: 143 DKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFVPDDVPPYVDTPVPIGY 202

Query: 208 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 267
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GPEGR VGVEHIPEL
Sbjct: 203 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRVVGVEHIPEL 262

Query: 268 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLK 327
           VASS+ENI KSAAAPLLKEGSLSLHV DGRQGW EC+PYDAIHVGAAAAEIPPALIDQLK
Sbjct: 263 VASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 322

Query: 328 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 352
           PGGRMVIPVGNIFQDL VVDKD+DGSV+IH+ETSVRYVPLTSREAQLRDN
Sbjct: 323 PGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSREAQLRDN 372

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BLAST of Carg20193 vs. NCBI nr
Match: XP_023515894.1 (protein-L-isoaspartate O-methyltransferase 1-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 478.4 bits (1230), Expect = 5.3e-131
Identity = 247/310 (79.68%), Postives = 261/310 (84.19%), Query Frame = 0


Query: 46  RFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSRMKAFVSNTCGFISSGFGS 105
           R PTALPP  SS PKTT T+I+S LPR  +      NCLLSRM+                
Sbjct: 38  RLPTALPPTTSSVPKTT-TTIISFLPRFLNPHFVTGNCLLSRMEV--------------- 97

Query: 106 ISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDR 165
                  SSL  PASEAVE+DKRFWSGSGI TNKEMV+NLQNYGVV SKKVSEVMES+DR
Sbjct: 98  -------SSLNGPASEAVEIDKRFWSGSGIGTNKEMVENLQNYGVVRSKKVSEVMESVDR 157

Query: 166 AFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC 225
           AFFVPD  PPYVDTP+PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC
Sbjct: 158 AFFVPDGVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC 217

Query: 226 FALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYD 285
           FALM+GPEGRAVGVEHIPELVASSI+NI KSAAAPLLKEGSLS+HV DGRQGW EC+PYD
Sbjct: 218 FALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKEGSLSVHVGDGRQGWAECAPYD 277

Query: 286 AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPL 345
           AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDL VVDKD DGS+NIH+ETSVRYVPL
Sbjct: 278 AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPL 324

Query: 346 TSREAQLRDN 352
           TSREAQL DN
Sbjct: 338 TSREAQLGDN 324

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BLAST of Carg20193 vs. ExPASy Swiss-Prot
Match: Q42539 (Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=PIMT1 PE=1 SV=3)

HSP 1 Score: 354.4 bits (908), Expect = 1.5e-96
Identity = 171/227 (75.33%), Postives = 198/227 (87.22%), Query Frame = 0


Query: 123 KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 182
           K+FWS S I  NK MV+NLQN+G+V S +V++ ME++DR  FV D +  YVD+P+ IGYN
Sbjct: 2   KQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYN 61

Query: 183 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 242
            TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+M+G EGRA+GVEHIPELV
Sbjct: 62  VTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELV 121

Query: 243 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 302
           ASS++NI  SAA+P LKEGSL++HV DGRQGW E +PYDAIHVGAAA EIP ALIDQLKP
Sbjct: 122 ASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKP 181

Query: 303 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLR 350
           GGR+VIPVGNIFQDL VVDK++DGSV+I +ETSVRYVPLTSREAQLR
Sbjct: 182 GGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLR 228

[Close]

BLAST of Carg20193 vs. ExPASy Swiss-Prot
Match: Q43209 (Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum OX=4565 GN=PCM PE=1 SV=1)

HSP 1 Score: 335.5 bits (859), Expect = 7.3e-91
Identity = 156/228 (68.42%), Postives = 191/228 (83.77%), Query Frame = 0


Query: 124 RFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNA 183
           +FW+   +  N  +V+ L+ YGVV + KV+EVME+IDRA FVP+   PY D+P+PIGYNA
Sbjct: 3   QFWAEGSLEKNNALVEYLKQYGVVRTDKVAEVMETIDRALFVPEGFTPYTDSPMPIGYNA 62

Query: 184 TISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVA 243
           TISAPHMHATCL+LL+ +LQPGM ALDVGSG+GYLTACFA+M+GPEGRAVG+EHIPELV 
Sbjct: 63  TISAPHMHATCLELLKDYLQPGMHALDVGSGSGYLTACFAMMVGPEGRAVGIEHIPELVV 122

Query: 244 SSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPG 303
           +S EN+ +SAAA L+K+GSLS HV+DGR GWP+ +PYDAIHVGAAA EIP  L++QLKPG
Sbjct: 123 ASTENVERSAAAALMKDGSLSFHVSDGRLGWPDAAPYDAIHVGAAAPEIPRPLLEQLKPG 182

Query: 304 GRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 352
           GRMVIPVG   QDL V+DK ADGS ++ N+ SVRYVPLTSR AQL+D+
Sbjct: 183 GRMVIPVGTYSQDLQVIDKSADGSTSVRNDASVRYVPLTSRSAQLQDS 230

[Close]

BLAST of Carg20193 vs. ExPASy Swiss-Prot
Match: Q64J17 (Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=PIMT2 PE=1 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 1.4e-89
Identity = 159/226 (70.35%), Postives = 188/226 (83.19%), Query Frame = 0


Query: 123 KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 182
           ++F SG+G +  + MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGYN
Sbjct: 81  EQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYN 140

Query: 183 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 242
           ATISAPHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPELV
Sbjct: 141 ATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELV 200

Query: 243 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 302
             SI+NI KS AA  LK+GSLSLHV DGR+GW E +PYDAIHVGAAA+EIP  L+DQLKP
Sbjct: 201 DMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKP 260

Query: 303 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQL 349
           GGRMVIP+G  FQ+L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 261 GGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 306

[Close]

BLAST of Carg20193 vs. ExPASy Swiss-Prot
Match: Q5F3N1 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus OX=9031 GN=PCMT1 PE=2 SV=3)

HSP 1 Score: 214.5 bits (545), Expect = 1.9e-54
Identity = 115/221 (52.04%), Postives = 149/221 (67.42%), Query Frame = 0


Query: 129 SGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAP 188
           SG A++ E++ NL+  G++ S KV EVM + DR  +   +  PY+D+P  IG+ ATISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKSDKVFEVMLATDRCHYAKYN--PYMDSPQSIGFQATISAP 64

Query: 189 HMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIEN 248
           HMHA  L+LL   L  G +ALDVGSG+G LTACF+ M+GP+G+ VG++HI ELV  SI N
Sbjct: 65  HMHAYALELLSDQLHEGAKALDVGSGSGILTACFSRMVGPKGQVVGIDHIKELVDDSINN 124

Query: 249 INKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVI 308
           + K     LL  G + L V DGR G+ E +PYDAIHVGAAA  +P ALIDQLKPGGR+++
Sbjct: 125 VKKDDPT-LLSSGRVKLIVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLIL 184

Query: 309 PVGNIFQDLMV--VDKDADGSVNIHNETSVRYVPLTSREAQ 348
           PVG    + M+   DK  DGSV +     V YVPLT +E Q
Sbjct: 185 PVGPAGGNQMLEQYDKLEDGSVKMKPLMGVIYVPLTDKEKQ 222

[Close]

BLAST of Carg20193 vs. ExPASy Swiss-Prot
Match: P80895 (Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa OX=9823 GN=PCMT1 PE=1 SV=3)

HSP 1 Score: 213.0 bits (541), Expect = 5.4e-54
Identity = 114/221 (51.58%), Postives = 148/221 (66.97%), Query Frame = 0


Query: 129 SGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAP 188
           SG A++ E++ NL+  G++ + KV EVM + DR+ +      PY+D+P  IG+ ATISAP
Sbjct: 5   SGGASHSELIHNLRKNGIIKTDKVFEVMLATDRSHYA--KCNPYMDSPQSIGFQATISAP 64

Query: 189 HMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIEN 248
           HMHA  L+LL   L  G +ALDVGSG+G LTACFA M+GP G+ +G++HI ELV  SI N
Sbjct: 65  HMHAYALELLFDQLHEGAKALDVGSGSGILTACFARMVGPSGKVIGIDHIKELVDDSINN 124

Query: 249 INKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVI 308
           + K     LL  G + L V DGR G+ E +PYDAIHVGAAA  +P ALIDQLKPGGR+++
Sbjct: 125 VRKDDPM-LLSSGRVQLVVGDGRMGYAEEAPYDAIHVGAAAPVVPQALIDQLKPGGRLIL 184

Query: 309 PVGNIFQDLMV--VDKDADGSVNIHNETSVRYVPLTSREAQ 348
           PVG    + M+   DK  DGSV +     V YVPLT +E Q
Sbjct: 185 PVGPAGGNQMLEQYDKLQDGSVKMKPLMGVIYVPLTDKEKQ 222

[Close]

BLAST of Carg20193 vs. ExPASy TrEMBL
Match: A0A5D3BFB7 (Protein-L-isoaspartate O-methyltransferase 1 isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold350G00920 PE=3 SV=1)

HSP 1 Score: 483.8 bits (1244), Expect = 6.1e-133
Identity = 256/350 (73.14%), Postives = 274/350 (78.29%), Query Frame = 0


Query: 28  PPICLWLPLLHHSPSLHRRFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSR 87
           P + L+  L  H     RR PTALPP  SS PKTT T+I+S LPR         NCLLSR
Sbjct: 23  PSLGLFFNLSCHHRRRRRRLPTALPPTTSSVPKTTVTTIISFLPRFLDPHFLTGNCLLSR 82

Query: 88  MKA---------FVSNTCGFISSGFGSISY-------------ADMFSSLTRPASEAVEL 147
           M+           V +    +S    S  Y                FSSL  PASEAVEL
Sbjct: 83  MEFQRGLSRVVWLVYSIVVCLSRDMQSFDYDIRWHTYPSELDGRGQFSSLNGPASEAVEL 142

Query: 148 DKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGY 207
           DKRFW+GSGI TNK+MV+ LQ YGVV SKKVSEVME+IDRAFFVPDD PPYVDTP+PIGY
Sbjct: 143 DKRFWTGSGIGTNKDMVEKLQTYGVVRSKKVSEVMENIDRAFFVPDDVPPYVDTPVPIGY 202

Query: 208 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPEL 267
           NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GPEGR VGVEHIPEL
Sbjct: 203 NATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPEGRVVGVEHIPEL 262

Query: 268 VASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLK 327
           VASS+ENI KSAAAPLLKEGSLSLHV DGRQGW EC+PYDAIHVGAAAAEIPPALIDQLK
Sbjct: 263 VASSMENIKKSAAAPLLKEGSLSLHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLK 322

Query: 328 PGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 352
           PGGRMVIPVGNIFQDL VVDKD+DGSV+IH+ETSVRYVPLTSREAQLRDN
Sbjct: 323 PGGRMVIPVGNIFQDLKVVDKDSDGSVSIHDETSVRYVPLTSREAQLRDN 372

[Close]

BLAST of Carg20193 vs. ExPASy TrEMBL
Match: A0A6J1E5Z6 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111429755 PE=3 SV=1)

HSP 1 Score: 475.3 bits (1222), Expect = 2.2e-130
Identity = 246/310 (79.35%), Postives = 260/310 (83.87%), Query Frame = 0


Query: 46  RFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSRMKAFVSNTCGFISSGFGS 105
           R PTALPP  SS PKTTAT I+S LPR  +      NCLLSRM+                
Sbjct: 34  RLPTALPPTTSSVPKTTAT-IISFLPRFLNPNFVTGNCLLSRMEV--------------- 93

Query: 106 ISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDR 165
                  SSL  PASEAVE+DKRFWSGSGI TNKEMV+NLQNYGVV SKKVSEVMES+DR
Sbjct: 94  -------SSLNGPASEAVEIDKRFWSGSGIGTNKEMVENLQNYGVVRSKKVSEVMESVDR 153

Query: 166 AFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC 225
           AFFVPD  PPYVDTP+PIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC
Sbjct: 154 AFFVPDGVPPYVDTPVPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC 213

Query: 226 FALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYD 285
           FALM+GP+GRAVGVEHIPELVASSI+NI KSAAAPLLKEGSLS+HV DGRQGW EC+PYD
Sbjct: 214 FALMVGPKGRAVGVEHIPELVASSIKNIEKSAAAPLLKEGSLSVHVGDGRQGWAECAPYD 273

Query: 286 AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPL 345
           AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDL VVDKD DGS+NI +ETSVRYVPL
Sbjct: 274 AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIRDETSVRYVPL 320

Query: 346 TSREAQLRDN 352
           TSREAQL DN
Sbjct: 334 TSREAQLGDN 320

[Close]

BLAST of Carg20193 vs. ExPASy TrEMBL
Match: A0A6J1JHZ4 (Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111484770 PE=3 SV=1)

HSP 1 Score: 473.4 bits (1217), Expect = 8.2e-130
Identity = 245/310 (79.03%), Postives = 260/310 (83.87%), Query Frame = 0


Query: 46  RFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSRMKAFVSNTCGFISSGFGS 105
           R PTALPP  SS PKTT T+I+  LPR  +      NCLLSRM+                
Sbjct: 36  RLPTALPPTASSVPKTT-TTIIPFLPRFLNPHFVTGNCLLSRMEV--------------- 95

Query: 106 ISYADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDR 165
                  SSL  PASEAVE+DKRFWSGSGI TNKEMV+NLQ+YGVV SKKVSEVMES+DR
Sbjct: 96  -------SSLNGPASEAVEIDKRFWSGSGIGTNKEMVENLQSYGVVRSKKVSEVMESVDR 155

Query: 166 AFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC 225
           AFFVPD  PPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC
Sbjct: 156 AFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTAC 215

Query: 226 FALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYD 285
           FALM+GPEGRAVGVEHIPELVASSI+NI KSAAAPLLK+GSLS+HV DGRQGW EC+PYD
Sbjct: 216 FALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKKGSLSVHVGDGRQGWAECAPYD 275

Query: 286 AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPL 345
           AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDL VVDKD DGS+NIH+ETSVRYVPL
Sbjct: 276 AIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVVDKDTDGSINIHDETSVRYVPL 322

Query: 346 TSREAQLRDN 352
           TSREAQL DN
Sbjct: 336 TSREAQLGDN 322

[Close]

BLAST of Carg20193 vs. ExPASy TrEMBL
Match: A0A6J1E0P2 (protein-L-isoaspartate O-methyltransferase 1-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111429755 PE=3 SV=1)

HSP 1 Score: 468.8 bits (1205), Expect = 2.0e-128
Identity = 249/338 (73.67%), Postives = 265/338 (78.40%), Query Frame = 0


Query: 46  RFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSRMK---------------- 105
           R PTALPP  SS PKTTAT I+S LPR  +      NCLLSRM+                
Sbjct: 34  RLPTALPPTTSSVPKTTAT-IISFLPRFLNPNFVTGNCLLSRMEFQGILFRVVCIVYSIL 93

Query: 106 ------------AFVSNTCGFISSGFGSISYADMFSSLTRPASEAVELDKRFWSGSGIAT 165
                           +TC  +  G          SSL  PASEAVE+DKRFWSGSGI T
Sbjct: 94  VYFPWDVQSFDNDIRRHTCPSVLDG--------QVSSLNGPASEAVEIDKRFWSGSGIGT 153

Query: 166 NKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHAT 225
           NKEMV+NLQNYGVV SKKVSEVMES+DRAFFVPD  PPYVDTP+PIGYNATISAPHMHAT
Sbjct: 154 NKEMVENLQNYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPVPIGYNATISAPHMHAT 213

Query: 226 CLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSA 285
           CLQLLEKHLQPGMRALDVGSGTGYLTACFALM+GP+GRAVGVEHIPELVASSI+NI KSA
Sbjct: 214 CLQLLEKHLQPGMRALDVGSGTGYLTACFALMVGPKGRAVGVEHIPELVASSIKNIEKSA 273

Query: 286 AAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNI 345
           AAPLLKEGSLS+HV DGRQGW EC+PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNI
Sbjct: 274 AAPLLKEGSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNI 333

Query: 346 FQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLRDN 352
           FQDL VVDKD DGS+NI +ETSVRYVPLTSREAQL DN
Sbjct: 334 FQDLKVVDKDTDGSINIRDETSVRYVPLTSREAQLGDN 362

[Close]

BLAST of Carg20193 vs. ExPASy TrEMBL
Match: A0A6J1JIJ1 (protein-L-isoaspartate O-methyltransferase 1-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111484770 PE=3 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 7.7e-128
Identity = 247/331 (74.62%), Postives = 265/331 (80.06%), Query Frame = 0


Query: 46  RFPTALPPINSSAPKTTATSIVSVLPRLRS----ASNCLLSRMKAFVSNTCGFISSGFGS 105
           R PTALPP  SS PKTT T+I+  LPR  +      NCLLSRM+ F       +   +  
Sbjct: 36  RLPTALPPTASSVPKTT-TTIIPFLPRFLNPHFVTGNCLLSRME-FQGILIRVVCVVYSI 95

Query: 106 ISY---------------------ADMFSSLTRPASEAVELDKRFWSGSGIATNKEMVKN 165
           + Y                         SSL  PASEAVE+DKRFWSGSGI TNKEMV+N
Sbjct: 96  LVYFPWDVQSFDNDIRRHACPSVLDGQVSSLNGPASEAVEIDKRFWSGSGIGTNKEMVEN 155

Query: 166 LQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQLLEK 225
           LQ+YGVV SKKVSEVMES+DRAFFVPD  PPYVDTPLPIGYNATISAPHMHATCLQLLEK
Sbjct: 156 LQSYGVVRSKKVSEVMESVDRAFFVPDGVPPYVDTPLPIGYNATISAPHMHATCLQLLEK 215

Query: 226 HLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAPLLKE 285
           HLQPGMRALDVGSGTGYLTACFALM+GPEGRAVGVEHIPELVASSI+NI KSAAAPLLK+
Sbjct: 216 HLQPGMRALDVGSGTGYLTACFALMVGPEGRAVGVEHIPELVASSIKNIEKSAAAPLLKK 275

Query: 286 GSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLMVV 345
           GSLS+HV DGRQGW EC+PYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDL VV
Sbjct: 276 GSLSVHVGDGRQGWAECAPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQDLKVV 335

Query: 346 DKDADGSVNIHNETSVRYVPLTSREAQLRDN 352
           DKD DGS+NIH+ETSVRYVPLTSREAQL DN
Sbjct: 336 DKDTDGSINIHDETSVRYVPLTSREAQLGDN 364

[Close]

BLAST of Carg20193 vs. TAIR 10
Match: AT3G48330.1 (protein-l-isoaspartate methyltransferase 1 )

HSP 1 Score: 354.4 bits (908), Expect = 1.1e-97
Identity = 171/227 (75.33%), Postives = 198/227 (87.22%), Query Frame = 0


Query: 123 KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 182
           K+FWS S I  NK MV+NLQN+G+V S +V++ ME++DR  FV D +  YVD+P+ IGYN
Sbjct: 2   KQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYN 61

Query: 183 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 242
            TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+M+G EGRA+GVEHIPELV
Sbjct: 62  VTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELV 121

Query: 243 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 302
           ASS++NI  SAA+P LKEGSL++HV DGRQGW E +PYDAIHVGAAA EIP ALIDQLKP
Sbjct: 122 ASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKP 181

Query: 303 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLR 350
           GGR+VIPVGNIFQDL VVDK++DGSV+I +ETSVRYVPLTSREAQLR
Sbjct: 182 GGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLR 228

[Close]

BLAST of Carg20193 vs. TAIR 10
Match: AT3G48330.2 (protein-l-isoaspartate methyltransferase 1 )

HSP 1 Score: 354.4 bits (908), Expect = 1.1e-97
Identity = 171/227 (75.33%), Postives = 198/227 (87.22%), Query Frame = 0


Query: 123 KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 182
           K+FWS S I  NK MV+NLQN+G+V S +V++ ME++DR  FV D +  YVD+P+ IGYN
Sbjct: 2   KQFWSPSSINKNKAMVENLQNHGIVTSDEVAKAMEAVDRGVFVTDRSSAYVDSPMSIGYN 61

Query: 183 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 242
            TISAPHMHA CLQLLEKHL+PGMR LDVGSGTGYLTACFA+M+G EGRA+GVEHIPELV
Sbjct: 62  VTISAPHMHAMCLQLLEKHLKPGMRVLDVGSGTGYLTACFAVMVGTEGRAIGVEHIPELV 121

Query: 243 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 302
           ASS++NI  SAA+P LKEGSL++HV DGRQGW E +PYDAIHVGAAA EIP ALIDQLKP
Sbjct: 122 ASSVKNIEASAASPFLKEGSLAVHVGDGRQGWAEFAPYDAIHVGAAAPEIPEALIDQLKP 181

Query: 303 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQLR 350
           GGR+VIPVGNIFQDL VVDK++DGSV+I +ETSVRYVPLTSREAQLR
Sbjct: 182 GGRLVIPVGNIFQDLQVVDKNSDGSVSIKDETSVRYVPLTSREAQLR 228

[Close]

BLAST of Carg20193 vs. TAIR 10
Match: AT5G50240.1 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 331.3 bits (848), Expect = 9.7e-91
Identity = 159/226 (70.35%), Postives = 188/226 (83.19%), Query Frame = 0


Query: 123 KRFWSGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYN 182
           ++F SG+G +  + MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGYN
Sbjct: 81  EQFQSGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYN 140

Query: 183 ATISAPHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELV 242
           ATISAPHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPELV
Sbjct: 141 ATISAPHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELV 200

Query: 243 ASSIENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKP 302
             SI+NI KS AA  LK+GSLSLHV DGR+GW E +PYDAIHVGAAA+EIP  L+DQLKP
Sbjct: 201 DMSIKNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKP 260

Query: 303 GGRMVIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQL 349
           GGRMVIP+G  FQ+L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 261 GGRMVIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 306

[Close]

BLAST of Carg20193 vs. TAIR 10
Match: AT5G50240.2 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 328.9 bits (842), Expect = 4.8e-90
Identity = 158/222 (71.17%), Postives = 185/222 (83.33%), Query Frame = 0


Query: 127 SGSGIATNKEMVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATIS 186
           SG+G +  + MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGYNATIS
Sbjct: 82  SGTGSSGKRGMVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYNATIS 141

Query: 187 APHMHATCLQLLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSI 246
           APHMHATCLQLLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPELV  SI
Sbjct: 142 APHMHATCLQLLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELVDMSI 201

Query: 247 ENINKSAAAPLLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRM 306
           +NI KS AA  LK+GSLSLHV DGR+GW E +PYDAIHVGAAA+EIP  L+DQLKPGGRM
Sbjct: 202 KNIEKSVAASFLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRM 261

Query: 307 VIPVGNIFQDLMVVDKDADGSVNIHNETSVRYVPLTSREAQL 349
           VIP+G  FQ+L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 262 VIPLGTYFQELKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 303

[Close]

BLAST of Carg20193 vs. TAIR 10
Match: AT5G50240.3 (protein-l-isoaspartate methyltransferase 2 )

HSP 1 Score: 323.9 bits (829), Expect = 1.6e-88
Identity = 155/212 (73.11%), Postives = 179/212 (84.43%), Query Frame = 0


Query: 137 MVKNLQNYGVVGSKKVSEVMESIDRAFFVPDDAPPYVDTPLPIGYNATISAPHMHATCLQ 196
           MV+NL+ YGV+ SK+V++VME++DR  FVP  +  YVDTP+PIGYNATISAPHMHATCLQ
Sbjct: 1   MVENLKRYGVISSKRVAQVMEALDRGLFVPVGSSAYVDTPVPIGYNATISAPHMHATCLQ 60

Query: 197 LLEKHLQPGMRALDVGSGTGYLTACFALMIGPEGRAVGVEHIPELVASSIENINKSAAAP 256
           LLE  L PGMRALDVGSGTGYLT CFALM+G EGR VGV+HIPELV  SI+NI KS AA 
Sbjct: 61  LLEDKLHPGMRALDVGSGTGYLTGCFALMVGAEGRVVGVDHIPELVDMSIKNIEKSVAAS 120

Query: 257 LLKEGSLSLHVADGRQGWPECSPYDAIHVGAAAAEIPPALIDQLKPGGRMVIPVGNIFQD 316
            LK+GSLSLHV DGR+GW E +PYDAIHVGAAA+EIP  L+DQLKPGGRMVIP+G  FQ+
Sbjct: 121 FLKKGSLSLHVGDGRKGWQEFAPYDAIHVGAAASEIPQPLLDQLKPGGRMVIPLGTYFQE 180

Query: 317 LMVVDKDADGSVNIHNETSVRYVPLTSREAQL 349
           L V+DK+ DGS+ +H ETSVRYVPLTSR  QL
Sbjct: 181 LKVIDKNEDGSIKVHTETSVRYVPLTSRVEQL 212

The following BLAST results are available for this feature:

BLAST of Carg20193 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7021419.1	6.5e-198	100.00	Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma su...	[more]
KAG6587436.1	2.1e-148	99.25	Protein-L-isoaspartate O-methyltransferase 1, partial [Cucurbita argyrosperma su...	[more]
XP_038879001.1	7.4e-133	70.91	protein-L-isoaspartate O-methyltransferase 1-like isoform X3 [Benincasa hispida]	[more]
TYJ98490.1	1.3e-132	73.14	protein-L-isoaspartate O-methyltransferase 1 isoform X1 [Cucumis melo var. makuw...	[more]
XP_023515894.1	5.3e-131	79.68	protein-L-isoaspartate O-methyltransferase 1-like isoform X2 [Cucurbita pepo sub...	[more]

BLAST of Carg20193 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q42539	1.5e-96	75.33	Protein-L-isoaspartate O-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q43209	7.3e-91	68.42	Protein-L-isoaspartate O-methyltransferase OS=Triticum aestivum OX=4565 GN=PCM P...	[more]
Q64J17	1.4e-89	70.35	Protein-L-isoaspartate O-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q5F3N1	1.9e-54	52.04	Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Gallus gallus OX=9031...	[more]
P80895	5.4e-54	51.58	Protein-L-isoaspartate(D-aspartate) O-methyltransferase OS=Sus scrofa OX=9823 GN...	[more]

BLAST of Carg20193 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5D3BFB7	6.1e-133	73.14	Protein-L-isoaspartate O-methyltransferase 1 isoform X1 OS=Cucumis melo var. mak...	[more]
A0A6J1E5Z6	2.2e-130	79.35	Protein-L-isoaspartate O-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1JHZ4	8.2e-130	79.03	Protein-L-isoaspartate O-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1E0P2	2.0e-128	73.67	protein-L-isoaspartate O-methyltransferase 1-like isoform X1 OS=Cucurbita moscha...	[more]
A0A6J1JIJ1	7.7e-128	74.62	protein-L-isoaspartate O-methyltransferase 1-like isoform X1 OS=Cucurbita maxima...	[more]

BLAST of Carg20193 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G48330.1	1.1e-97	75.33	protein-l-isoaspartate methyltransferase 1	[more]
AT3G48330.2	1.1e-97	75.33	protein-l-isoaspartate methyltransferase 1	[more]
AT5G50240.1	9.7e-91	70.35	protein-l-isoaspartate methyltransferase 2	[more]
AT5G50240.2	4.8e-90	71.17	protein-l-isoaspartate methyltransferase 2	[more]
AT5G50240.3	1.6e-88	73.11	protein-l-isoaspartate methyltransferase 2	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	TIGRFAM	TIGR00080	TIGR00080	coord: 133..347 e-value: 4.2E-64 score: 214.3
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	PANTHER	PTHR11579	PROTEIN-L-ISOASPARTATE O-METHYLTRANSFERASE	coord: 107..351
IPR000682	Protein-L-isoaspartate(D-aspartate) O-methyltransferase	PROSITE	PS01279	PCMT	coord: 268..283
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 126..351 e-value: 4.1E-79 score: 267.5
None	No IPR available	PFAM	PF01135	PCMT	coord: 133..345 e-value: 1.3E-83 score: 279.9
None	No IPR available	PANTHER	PTHR11579:SF26	L-ISOASPARTATE METHYLTRANSFERASE	coord: 107..351
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 207..310 e-value: 4.44866E-7 score: 45.8839
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 133..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg20193-RA	Carg20193-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006479	protein methylation
biological_process	GO:0006464	cellular protein modification process
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0004719	protein-L-isoaspartate (D-aspartate) O-methyltransferase activity