Carg19945 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19945
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description9-cis-epoxycarotenoid dioxygenase, chloroplastic
LocationCarg_Chr13: 6685631 .. 6687301 (-)
RNA-Seq ExpressionCarg19945
SyntenyCarg19945
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCTATGGCACTCTCTTCAAGTTCAGGCTCACCCCATTTGGGCTTGGGTTTGGGCTTTCCTACTAGGCCCATTAGGAAAAAGCCCAATCTGAGAAACGAGGTGATTTCCTGCACACTAAACTCCCCGTCTGTGTTCCATTACCCGAAACAGCCCAATAAACGGCCGAGAATCACGGAAGACGACGCTACCAAATGTCGTCATCATCCTCCCCGCTGGAATTTACTGCAAAAGGGAGCTTCCATGGCTTTTGACATCCTGGAACAAGCCTTGCTGAATTACGACAACCTTAATCTTCCTAAGAACTTCGACCCACATGTCCAAATCGCTGGGAACTACGCTCCGGTACCGGAACAATCGGTTTCTCACTCTCTTCCGGTCACTGGCGCCATCCCTGATTGCATCGATGGTGTCTATCTCCGAAACGGCGCAAACCCATTATTCGAACCGCTCGGAGGTCATCATCTGTTTGACGGTGACGGTATGATTCACGCTGTTACAATCGGCGGCGGATGTGCCAGATATGCCTGCAGGTTCACTGAAACAGAGAGGCTTGTTCAAGAAAGGTCCATTGGCCGGCCGGTTTTTCCCAAGACCATTGGTGAGCTCCATGGACATTCCGGCATTGCTCGTCTTCTTCTCTTCTACGCTCGAGGACTGTTTGGGTTAATTGACCACCGTCATGGCACCGGAGTCGCTAACGCCGGCTTGGTCTACTTCAATGGTCATCTTTTGGCTATGTCAGAAGAAGACCTTCCGTATCACGTTCGTATCACCCCCTCCGGCGATCTGGTCACTGCCGGACGGTATAATTTCGAGGGTCAGCTCGATTCTCCGATGGTCGCTCACCCCAAAATCGACCCGGTTTCCGGTGAGCTCTTTGCCCTCAGCTACAATTTCAGTCACAAGCCGTATTTAACTCACTTCCGATTCTCACCGGATGGGAAAATGTCGCCTAAAATCGAAATTCCGGTGGAAATTCCGACAATGATGCACGATTTCGCCATAACAGAGAATTTTGTCGTGATCCCAGATCAACAAGTCGTTTTCAATCTTCGGAAAATGATCAACGGTGGATCTCCGGTAATGTACGACGACAAAAAGACGCCCCGATTTGGGTTCTTACCGAAAAACGCAACGGATTCATCGAATCTCATATGGGTTGATTCGCCGGCTGATACGTTCTGTTTCCATCTGTGGAATGCGTGGGAAGAAACAGAGTCAAACGAGGTCGTTGTAATCGGGTCCTGCATGACCCCACCGGATTCCATTTTCAACGGACAAGACGAGGATTTGAAGGCGATTCTAACGGAAATCCGGTTCAATTTGGTTACCGGAGATTCAGCTCGCCGGCCGATCGTTTCGAAACCAGAGGAGGTGAACCTGGAAGTCGGAATGGTGAACCAGAGCCTTGTCGGGAGGAAAACCCGGTACGCTTATTTGGCTATTACAGAGCCATGGCCGAAGGCGTGTGGGTTCGCGAAAGTGGATCTCTGGTCGGGAGAGATAAAGAAGTACATTTACGGCGATCAACGGTACGGCGGAGAGCCATACTTTTTGGGGAGAGAAGTATACTCCGATTGTGAAAGAGAGGACGATGGGTTCATAATGGTGTTTGGTGCACGACGAGAAGAAATGGAGATCGGAGCTTCAGATCGTGAATGCCATTGA

mRNA sequence

ATGGCTATGGCACTCTCTTCAAGTTCAGGCTCACCCCATTTGGGCTTGGGTTTGGGCTTTCCTACTAGGCCCATTAGGAAAAAGCCCAATCTGAGAAACGAGGTGATTTCCTGCACACTAAACTCCCCGTCTGTGTTCCATTACCCGAAACAGCCCAATAAACGGCCGAGAATCACGGAAGACGACGCTACCAAATGTCGTCATCATCCTCCCCGCTGGAATTTACTGCAAAAGGGAGCTTCCATGGCTTTTGACATCCTGGAACAAGCCTTGCTGAATTACGACAACCTTAATCTTCCTAAGAACTTCGACCCACATGTCCAAATCGCTGGGAACTACGCTCCGGTACCGGAACAATCGGTTTCTCACTCTCTTCCGGTCACTGGCGCCATCCCTGATTGCATCGATGGTGTCTATCTCCGAAACGGCGCAAACCCATTATTCGAACCGCTCGGAGGTCATCATCTGTTTGACGGTGACGGTATGATTCACGCTGTTACAATCGGCGGCGGATGTGCCAGATATGCCTGCAGGTTCACTGAAACAGAGAGGCTTGTTCAAGAAAGGTCCATTGGCCGGCCGGTTTTTCCCAAGACCATTGGTGAGCTCCATGGACATTCCGGCATTGCTCGTCTTCTTCTCTTCTACGCTCGAGGACTGTTTGGGTTAATTGACCACCGTCATGGCACCGGAGTCGCTAACGCCGGCTTGGTCTACTTCAATGGTCATCTTTTGGCTATGTCAGAAGAAGACCTTCCGTATCACGTTCGTATCACCCCCTCCGGCGATCTGGTCACTGCCGGACGGTATAATTTCGAGGGTCAGCTCGATTCTCCGATGGTCGCTCACCCCAAAATCGACCCGGTTTCCGGTGAGCTCTTTGCCCTCAGCTACAATTTCAGTCACAAGCCGTATTTAACTCACTTCCGATTCTCACCGGATGGGAAAATGTCGCCTAAAATCGAAATTCCGGTGGAAATTCCGACAATGATGCACGATTTCGCCATAACAGAGAATTTTGTCGTGATCCCAGATCAACAAGTCGTTTTCAATCTTCGGAAAATGATCAACGGTGGATCTCCGGTAATGTACGACGACAAAAAGACGCCCCGATTTGGGTTCTTACCGAAAAACGCAACGGATTCATCGAATCTCATATGGGTTGATTCGCCGGCTGATACGTTCTGTTTCCATCTGTGGAATGCGTGGGAAGAAACAGAGTCAAACGAGGTCGTTGTAATCGGGTCCTGCATGACCCCACCGGATTCCATTTTCAACGGACAAGACGAGGATTTGAAGGCGATTCTAACGGAAATCCGGTTCAATTTGGTTACCGGAGATTCAGCTCGCCGGCCGATCGTTTCGAAACCAGAGGAGGTGAACCTGGAAGTCGGAATGGTGAACCAGAGCCTTGTCGGGAGGAAAACCCGGTACGCTTATTTGGCTATTACAGAGCCATGGCCGAAGGCGTGTGGGTTCGCGAAAGTGGATCTCTGGTCGGGAGAGATAAAGAAGTACATTTACGGCGATCAACGGTACGGCGGAGAGCCATACTTTTTGGGGAGAGAAGTATACTCCGATTGTGAAAGAGAGGACGATGGGTTCATAATGGTGTTTGGTGCACGACGAGAAGAAATGGAGATCGGAGCTTCAGATCGTGAATGCCATTGA

Coding sequence (CDS)

ATGGCTATGGCACTCTCTTCAAGTTCAGGCTCACCCCATTTGGGCTTGGGTTTGGGCTTTCCTACTAGGCCCATTAGGAAAAAGCCCAATCTGAGAAACGAGGTGATTTCCTGCACACTAAACTCCCCGTCTGTGTTCCATTACCCGAAACAGCCCAATAAACGGCCGAGAATCACGGAAGACGACGCTACCAAATGTCGTCATCATCCTCCCCGCTGGAATTTACTGCAAAAGGGAGCTTCCATGGCTTTTGACATCCTGGAACAAGCCTTGCTGAATTACGACAACCTTAATCTTCCTAAGAACTTCGACCCACATGTCCAAATCGCTGGGAACTACGCTCCGGTACCGGAACAATCGGTTTCTCACTCTCTTCCGGTCACTGGCGCCATCCCTGATTGCATCGATGGTGTCTATCTCCGAAACGGCGCAAACCCATTATTCGAACCGCTCGGAGGTCATCATCTGTTTGACGGTGACGGTATGATTCACGCTGTTACAATCGGCGGCGGATGTGCCAGATATGCCTGCAGGTTCACTGAAACAGAGAGGCTTGTTCAAGAAAGGTCCATTGGCCGGCCGGTTTTTCCCAAGACCATTGGTGAGCTCCATGGACATTCCGGCATTGCTCGTCTTCTTCTCTTCTACGCTCGAGGACTGTTTGGGTTAATTGACCACCGTCATGGCACCGGAGTCGCTAACGCCGGCTTGGTCTACTTCAATGGTCATCTTTTGGCTATGTCAGAAGAAGACCTTCCGTATCACGTTCGTATCACCCCCTCCGGCGATCTGGTCACTGCCGGACGGTATAATTTCGAGGGTCAGCTCGATTCTCCGATGGTCGCTCACCCCAAAATCGACCCGGTTTCCGGTGAGCTCTTTGCCCTCAGCTACAATTTCAGTCACAAGCCGTATTTAACTCACTTCCGATTCTCACCGGATGGGAAAATGTCGCCTAAAATCGAAATTCCGGTGGAAATTCCGACAATGATGCACGATTTCGCCATAACAGAGAATTTTGTCGTGATCCCAGATCAACAAGTCGTTTTCAATCTTCGGAAAATGATCAACGGTGGATCTCCGGTAATGTACGACGACAAAAAGACGCCCCGATTTGGGTTCTTACCGAAAAACGCAACGGATTCATCGAATCTCATATGGGTTGATTCGCCGGCTGATACGTTCTGTTTCCATCTGTGGAATGCGTGGGAAGAAACAGAGTCAAACGAGGTCGTTGTAATCGGGTCCTGCATGACCCCACCGGATTCCATTTTCAACGGACAAGACGAGGATTTGAAGGCGATTCTAACGGAAATCCGGTTCAATTTGGTTACCGGAGATTCAGCTCGCCGGCCGATCGTTTCGAAACCAGAGGAGGTGAACCTGGAAGTCGGAATGGTGAACCAGAGCCTTGTCGGGAGGAAAACCCGGTACGCTTATTTGGCTATTACAGAGCCATGGCCGAAGGCGTGTGGGTTCGCGAAAGTGGATCTCTGGTCGGGAGAGATAAAGAAGTACATTTACGGCGATCAACGGTACGGCGGAGAGCCATACTTTTTGGGGAGAGAAGTATACTCCGATTGTGAAAGAGAGGACGATGGGTTCATAATGGTGTTTGGTGCACGACGAGAAGAAATGGAGATCGGAGCTTCAGATCGTGAATGCCATTGA

Protein sequence

MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFGARREEMEIGASDRECH
Homology
BLAST of Carg19945 vs. NCBI nr
Match: KAG7019511.1 (putative 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1174.5 bits (3037), Expect = 0.0e+00
Identity = 556/556 (100.00%), Postives = 556/556 (100.00%), Query Frame = 0

Query: 1   MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60
           MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE
Sbjct: 1   MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60

Query: 61  DDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120
           DDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS
Sbjct: 61  DDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120

Query: 121 VSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFT 180
           VSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFT
Sbjct: 121 VSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFT 180

Query: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240
           ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF
Sbjct: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240

Query: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNF 300
           NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNF
Sbjct: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNF 300

Query: 301 SHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360
           SHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS
Sbjct: 301 SHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360

Query: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420
           PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP
Sbjct: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420

Query: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480
           PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY
Sbjct: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480

Query: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFG 540
           LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFG
Sbjct: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFG 540

Query: 541 ARREEMEIGASDRECH 557
           ARREEMEIGASDRECH
Sbjct: 541 ARREEMEIGASDRECH 556

BLAST of Carg19945 vs. NCBI nr
Match: XP_022927145.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 530/538 (98.51%), Postives = 533/538 (99.07%), Query Frame = 0

Query: 1   MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60
           MAMALSSSS S HLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE
Sbjct: 1   MAMALSSSSASLHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60

Query: 61  DDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120
           DDA+KCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS
Sbjct: 61  DDASKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120

Query: 121 VSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFT 180
           VSHSLPVTGAIPDCI+GVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGC  YACRFT
Sbjct: 121 VSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCTSYACRFT 180

Query: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240
           ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF
Sbjct: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240

Query: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNF 300
           NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDP SGELFALSYNF
Sbjct: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPDSGELFALSYNF 300

Query: 301 SHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360
           +HKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS
Sbjct: 301 THKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360

Query: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420
           PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP
Sbjct: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420

Query: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480
           PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY
Sbjct: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480

Query: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMV 539
           LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMV
Sbjct: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMV 538

BLAST of Carg19945 vs. NCBI nr
Match: XP_023520051.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1110.1 bits (2870), Expect = 0.0e+00
Identity = 524/539 (97.22%), Postives = 531/539 (98.52%), Query Frame = 0

Query: 1   MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60
           MAMALSSSS SPHLG GLGFPTRPIRKKPNLRNEVISC LNSPSVFHYPKQP+KRPRITE
Sbjct: 1   MAMALSSSSASPHLGFGLGFPTRPIRKKPNLRNEVISCALNSPSVFHYPKQPSKRPRITE 60

Query: 61  DDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120
           DDA+KCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS
Sbjct: 61  DDASKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120

Query: 121 VSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFT 180
           VSHSLPVTGAIPDCI+GVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCA YACRFT
Sbjct: 121 VSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCASYACRFT 180

Query: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240
           ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF
Sbjct: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240

Query: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNF 300
           NG LLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDP SGELFALSYNF
Sbjct: 241 NGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPDSGELFALSYNF 300

Query: 301 SHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360
           +HKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQ+VFNLRKMINGGS
Sbjct: 301 THKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQIVFNLRKMINGGS 360

Query: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420
           PVMYD+KKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP
Sbjct: 361 PVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420

Query: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480
           PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSK EEVNLEVGMVNQSLVGRKTRYAY
Sbjct: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGMVNQSLVGRKTRYAY 480

Query: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVF 540
           LAITEPWPKACGFAKVDLWSGEIKKYIYGDQ YGGEPYFLGR+VYSDCEREDDGFIMVF
Sbjct: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQLYGGEPYFLGRDVYSDCEREDDGFIMVF 539

BLAST of Carg19945 vs. NCBI nr
Match: XP_023001406.1 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 520/552 (94.20%), Postives = 528/552 (95.65%), Query Frame = 0

Query: 1   MAMALSSSSGSPH--------------LGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVF 60
           MAMALSSSS SPH              +G GLGF TRPIRKKPNLRNEVI+C LNSPSVF
Sbjct: 1   MAMALSSSSASPHFPFDRRLLEISTVAMGFGLGFLTRPIRKKPNLRNEVIACALNSPSVF 60

Query: 61  HYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPH 120
           HYPKQPNKRPRITEDDA+KCRH PPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPH
Sbjct: 61  HYPKQPNKRPRITEDDASKCRHRPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPH 120

Query: 121 VQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAV 180
           VQIAGNYAPVPEQSVSHSLPVTGAIPDCI+GVYLRNGANPLF+PLGGHHLFDGDGMIHAV
Sbjct: 121 VQIAGNYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFDPLGGHHLFDGDGMIHAV 180

Query: 181 TIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDH 240
           TIGGGCA YACRFTETERLVQERSIGRP+FPKTIGELHGHSGIARLLLFYARGLFGLIDH
Sbjct: 181 TIGGGCASYACRFTETERLVQERSIGRPIFPKTIGELHGHSGIARLLLFYARGLFGLIDH 240

Query: 241 RHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKI 300
           RHGTGVANAGLVYFNG LLAMSEEDLPYHVRITPSGDLVTAGRYNFEG LDSPMVAHPKI
Sbjct: 241 RHGTGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGHLDSPMVAHPKI 300

Query: 301 DPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ 360
           DP SGELFALSYNF+HKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPDSGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET 420
           QVVFNLRKMINGGSPVMYD+KKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET
Sbjct: 361 QVVFNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET 420

Query: 421 ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGM 480
           ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSK EEVNLEVGM
Sbjct: 421 ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGM 480

Query: 481 VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS 539
           VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS
Sbjct: 481 VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS 540

BLAST of Carg19945 vs. NCBI nr
Match: KAG6583893.1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 966.5 bits (2497), Expect = 1.0e-277
Identity = 457/458 (99.78%), Postives = 457/458 (99.78%), Query Frame = 0

Query: 82  MAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLR 141
           MAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLR
Sbjct: 1   MAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLR 60

Query: 142 NGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIG 201
           NGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIG
Sbjct: 61  NGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIG 120

Query: 202 ELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPS 261
           ELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPS
Sbjct: 121 ELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPS 180

Query: 262 GDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKI 321
           GDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKI
Sbjct: 181 GDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKI 240

Query: 322 EIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATD 381
           EIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATD
Sbjct: 241 EIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATD 300

Query: 382 SSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRF 441
           SSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRF
Sbjct: 301 SSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRF 360

Query: 442 NLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSG 501
           NLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSG
Sbjct: 361 NLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSG 420

Query: 502 EIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVF 540
           EIKKYIYGDQ YGGEPYFLGREVYSDCEREDDGFIMVF
Sbjct: 421 EIKKYIYGDQLYGGEPYFLGREVYSDCEREDDGFIMVF 458

BLAST of Carg19945 vs. ExPASy Swiss-Prot
Match: K4CJJ1 (9-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED2 PE=2 SV=1)

HSP 1 Score: 725.3 bits (1871), Expect = 5.2e-208
Identity = 350/534 (65.54%), Postives = 421/534 (78.84%), Query Frame = 0

Query: 7   SSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITEDDATKC 66
           S S SP     L F T P  K  +++N   + T    ++   PKQ N  P+       + 
Sbjct: 11  SHSFSPSTSYSLDF-TLP-SKSISMKNHTTTKTKIHSALLTLPKQ-NNTPK------NQP 70

Query: 67  RHHPPRWNLLQKGASMAFDILEQALLNYDNLN-LPKNFDPHVQIAGNYAPVPEQSVSHSL 126
           +     WN  QK A+ A DI+E AL++ +  N LPK  DP VQIAGN+APVPEQSV H+L
Sbjct: 71  QFQTSHWNFFQKAAAKALDIVESALVSRELQNPLPKTADPRVQIAGNFAPVPEQSVRHNL 130

Query: 127 PVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERL 186
           PVTG IPDCI+GVY+RNGANPLFEP+ GHHLFDGDGM+HAVT+  G   Y+CRFTETERL
Sbjct: 131 PVTGTIPDCINGVYVRNGANPLFEPVAGHHLFDGDGMVHAVTVENGSVSYSCRFTETERL 190

Query: 187 VQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLL 246
           VQER +G PVFPK IGELHGHSGIARLLLFYARG+FGL+DH HGTGVANAGLV+FN  LL
Sbjct: 191 VQERELGHPVFPKAIGELHGHSGIARLLLFYARGVFGLVDHSHGTGVANAGLVFFNNRLL 250

Query: 247 AMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPY 306
           AMSE+D+PYHV++ PSGDL T GRYNF+ QL S M+AHPKIDPVSGELFALSY+   KPY
Sbjct: 251 AMSEDDVPYHVQVLPSGDLQTVGRYNFDDQLKSTMIAHPKIDPVSGELFALSYDVVQKPY 310

Query: 307 LTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYD 366
           L  F+FSPDG+ SP +EIP+++PTMMHDFAITEN+VVIPDQQVVF L++MI GGSPV+YD
Sbjct: 311 LKSFKFSPDGEKSPDVEIPLDVPTMMHDFAITENYVVIPDQQVVFKLQEMIKGGSPVIYD 370

Query: 367 DKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIF 426
             K  RFG LPKNA +S N+IWV+S A+TFCFHLWNAWEE E++EV+VIGSCMTPPDSIF
Sbjct: 371 KNKKSRFGILPKNAENSENIIWVES-AETFCFHLWNAWEEPETDEVIVIGSCMTPPDSIF 430

Query: 427 NGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITE 486
           N  +E+LK++L+EIR NL TG+S RR ++S  ++VNLE GMVN++ +GRKT++AYLAI E
Sbjct: 431 NECNENLKSVLSEIRLNLKTGESTRRQLLSPSDQVNLEAGMVNRNKLGRKTQFAYLAIAE 490

Query: 487 PWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVF 540
           PWPK  GFAKVDL +GEIKK+IYGD+RYGGEP FL R V S  E+EDDG+I+ F
Sbjct: 491 PWPKVSGFAKVDLSTGEIKKHIYGDKRYGGEPLFLPRNVNS--EKEDDGYILAF 532

BLAST of Carg19945 vs. ExPASy Swiss-Prot
Match: O24023 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum OX=4081 GN=NCED1 PE=1 SV=2)

HSP 1 Score: 696.0 bits (1795), Expect = 3.3e-199
Identity = 331/525 (63.05%), Postives = 402/525 (76.57%), Query Frame = 0

Query: 25  IRKKPNLRNEVISCTLNSPSVFHYPKQP--------NKRPRITEDDATKCRHHPPRWNLL 84
           ++ + N ++  I+ +L +P + H+PKQ         N      +++         +WNL+
Sbjct: 35  LKNQHNRQSLNINSSLQAPPILHFPKQSSNYQTPKNNTISHPKQENNNSSSSSTSKWNLV 94

Query: 85  QKGASMAFDILEQALLNYD-NLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCI 144
           QK A+MA D +E AL  ++    LPK  DP VQI+GN+APVPE  V  SLPVTG IP C+
Sbjct: 95  QKAAAMALDAVESALTKHELEHPLPKTADPRVQISGNFAPVPENPVCQSLPVTGKIPKCV 154

Query: 145 DGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPV 204
            GVY+RNGANPLFEP  GHH FDGDGM+HAV    G A YACRFTETERLVQE+++GRPV
Sbjct: 155 QGVYVRNGANPLFEPTAGHHFFDGDGMVHAVQFKNGSASYACRFTETERLVQEKALGRPV 214

Query: 205 FPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYH 264
           FPK IGELHGHSGIARL+LFYARGLFGL+DH  GTGVANAGLVYFN  LLAMSE+DLPYH
Sbjct: 215 FPKAIGELHGHSGIARLMLFYARGLFGLVDHSKGTGVANAGLVYFNNRLLAMSEDDLPYH 274

Query: 265 VRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDG 324
           V++TP+GDL T GR++F+GQL S M+AHPK+DPVSGELFALSY+   KPYL +FRFS +G
Sbjct: 275 VKVTPTGDLKTEGRFDFDGQLKSTMIAHPKLDPVSGELFALSYDVIQKPYLKYFRFSKNG 334

Query: 325 KMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFL 384
           + S  +EIPVE PTMMHDFAITENFVVIPDQQVVF + +MI GGSPV+YD  K  RFG L
Sbjct: 335 EKSNDVEIPVEDPTMMHDFAITENFVVIPDQQVVFKMSEMIRGGSPVVYDKNKVSRFGIL 394

Query: 385 PKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAI 444
            K A D S+L WV+ P D FCFHLWNAWEE E++E+VVIGSCMTPPDSIFN  DE LK++
Sbjct: 395 DKYAKDGSDLKWVEVP-DCFCFHLWNAWEEAETDEIVVIGSCMTPPDSIFNECDEGLKSV 454

Query: 445 LTEIRFNLVTGDSARRPIVSKP-EEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFA 504
           L+EIR NL TG S R+ I+  P E+VNLE GMVN++ +GRKT YAYLAI EPWPK  GFA
Sbjct: 455 LSEIRLNLKTGKSTRKSIIENPDEQVNLEAGMVNRNKLGRKTEYAYLAIAEPWPKVSGFA 514

Query: 505 KVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVF 540
           KV+L++GE++K+IYGD +YGGEP FL R+  S  + EDDG+I+ F
Sbjct: 515 KVNLFTGEVEKFIYGDNKYGGEPLFLPRDPNS--KEEDDGYILAF 556

BLAST of Carg19945 vs. ExPASy Swiss-Prot
Match: Q9C6Z1 (Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED5 PE=1 SV=1)

HSP 1 Score: 690.3 bits (1780), Expect = 1.8e-197
Identity = 332/521 (63.72%), Postives = 397/521 (76.20%), Query Frame = 0

Query: 28  KPNLRNEVISCTLNSPSVFHYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDIL 87
           KP  R    +   ++P++ ++P  P+  P I E D +       RWN LQ+ AS A D  
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKDTS-------RWNPLQRAASAALDFA 97

Query: 88  EQALLNYDNLN-LPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANP 147
           E ALL  +    LPK  DP  QI+GNYAPVPEQSV  SL V G IPDCIDGVYLRNGANP
Sbjct: 98  ETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANP 157

Query: 148 LFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGH 207
           LFEP+ GHHLFDGDGM+HAV I  G A Y+CRFTETERLVQE+ +G P+FPK IGELHGH
Sbjct: 158 LFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGH 217

Query: 208 SGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVT 267
           SGIARL+LFYARGLFGL++H++GTGVANAGLVYF+  LLAMSE+DLPY VR+T +GDL T
Sbjct: 218 SGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLET 277

Query: 268 AGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVE 327
            GR++F+GQL S M+AHPKIDPV+ ELFALSY+   KPYL +F+FSP+G+ SP +EIP+ 
Sbjct: 278 IGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLA 337

Query: 328 IPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLI 387
            PTMMHDFAITENFVVIPDQQVVF L  M  G SPV YD +K  RFG LP+NA D+S ++
Sbjct: 338 SPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMV 397

Query: 388 WVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTG 447
           WV+SP +TFCFHLWNAWE  E++EVVVIGSCMTP DSIFN  DE L ++L+EIR NL TG
Sbjct: 398 WVESP-ETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTG 457

Query: 448 DSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKY 507
            S RR I+    ++NLE GMVN++L+GRKTRYAYLAI EPWPK  GFAKVDL +GE+K +
Sbjct: 458 KSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNH 517

Query: 508 IYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFGARREEME 548
            YG ++YGGEP+FL R + SD   EDDG+IM F    E  E
Sbjct: 518 FYGGKKYGGEPFFLPRGLESD--GEDDGYIMSFVHDEESWE 548

BLAST of Carg19945 vs. ExPASy Swiss-Prot
Match: Q9M6E8 (9-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=3885 GN=NCED1 PE=2 SV=1)

HSP 1 Score: 688.7 bits (1776), Expect = 5.3e-197
Identity = 334/558 (59.86%), Postives = 411/558 (73.66%), Query Frame = 0

Query: 5   LSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPK--QPNK-------- 64
           L+S+S SP   L       P +K+ +     I+C+L +    HYPK  QP          
Sbjct: 25  LASTSSSPTTLL-------PFKKRSSSNTNTITCSLQT---LHYPKQYQPTSTSTTTTPT 84

Query: 65  --RPRITEDDATKCRH----------HPPRWNLLQKGASMAFDILEQALLNYDNLN-LPK 124
             +P  T    T  R            P +WN LQK A+   D++E AL+++++ + LPK
Sbjct: 85  PIKPTTTTTTTTPHRETKPLSDTKQPFPQKWNFLQKAAATGLDMVETALVSHESKHPLPK 144

Query: 125 NFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDG 184
             DP VQIAGN+APVPE +   +LPV G IP CIDGVY+RNGANPL+EP+ GHH FDGDG
Sbjct: 145 TADPKVQIAGNFAPVPEHAADQALPVVGKIPKCIDGVYVRNGANPLYEPVAGHHFFDGDG 204

Query: 185 MIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLF 244
           M+HAV    G A YACRFTET+RL QE+S+GRPVFPK IGELHGHSGIARLLLFYAR LF
Sbjct: 205 MVHAVKFTNGAASYACRFTETQRLAQEKSLGRPVFPKAIGELHGHSGIARLLLFYARSLF 264

Query: 245 GLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMV 304
            L+D  HG GVANAGLVYFN HLLAMSE+DLPYHVRIT +GDL T GRY+F GQL+S M+
Sbjct: 265 QLVDGSHGMGVANAGLVYFNNHLLAMSEDDLPYHVRITSNGDLTTVGRYDFNGQLNSTMI 324

Query: 305 AHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFV 364
           AHPK+DPV+G+L ALSY+   KPYL +FRFS DG  SP +EIP++ PTMMHDFAITENFV
Sbjct: 325 AHPKLDPVNGDLHALSYDVVQKPYLKYFRFSADGVKSPDVEIPLKEPTMMHDFAITENFV 384

Query: 365 VIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWN 424
           V+PDQQVVF L +MI GGSPV+YD  KT RFG L KNA D++ + W+D+P + FCFHLWN
Sbjct: 385 VVPDQQVVFKLTEMITGGSPVVYDKNKTSRFGILDKNAKDANAMRWIDAP-ECFCFHLWN 444

Query: 425 AWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVN 484
           AWEE E++E+VVIGSCMTP DSIFN  DE LK++L+EIR NL TG S RRPI+S  E+VN
Sbjct: 445 AWEEPETDEIVVIGSCMTPADSIFNECDESLKSVLSEIRLNLRTGKSTRRPIISDAEQVN 504

Query: 485 LEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLG 540
           LE GMVN++ +GRKT++AYLA+ EPWPK  GFAKVDL+SGE++KY+YG++++GGEP FL 
Sbjct: 505 LEAGMVNRNKLGRKTQFAYLALAEPWPKVSGFAKVDLFSGEVQKYMYGEEKFGGEPLFL- 564

BLAST of Carg19945 vs. ExPASy Swiss-Prot
Match: Q9LRR7 (9-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=NCED3 PE=1 SV=1)

HSP 1 Score: 677.9 bits (1748), Expect = 9.4e-194
Identity = 321/506 (63.44%), Postives = 392/506 (77.47%), Query Frame = 0

Query: 36  ISCTLNSPSVFHYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYD 95
           +S  L++P   H+PKQ +  P I      K   +  + NL Q+ A+ A D  E  L++++
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAK-ESNTKQMNLFQRAAAAALDAAEGFLVSHE 109

Query: 96  NLN-LPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGH 155
            L+ LPK  DP VQIAGN+APV EQ V  +LPV G +PD I GVY+RNGANPL EP+ GH
Sbjct: 110 KLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGH 169

Query: 156 HLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLL 215
           H FDGDGM+HAV    G A YACRFT+T R VQER +GRPVFPK IGELHGH+GIARL+L
Sbjct: 170 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 229

Query: 216 FYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEG 275
           FYAR   G++D  HGTGVANAGLVYFNG LLAMSE+DLPY V+ITP+GDL T GR++F+G
Sbjct: 230 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDG 289

Query: 276 QLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDF 335
           QL+S M+AHPK+DP SGELFALSY+   KPYL +FRFSPDG  SP +EI ++ PTMMHDF
Sbjct: 290 QLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDF 349

Query: 336 AITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADT 395
           AITENFVV+PDQQVVF L +MI GGSPV+YD  K  RFG L K A DSSN+ W+D+P D 
Sbjct: 350 AITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAP-DC 409

Query: 396 FCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIV 455
           FCFHLWNAWEE E++EVVVIGSCMTPPDSIFN  DE+LK++L+EIR NL TG+S RRPI+
Sbjct: 410 FCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPII 469

Query: 456 S-KPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRY 515
           S + ++VNLE GMVN++++GRKT++AYLA+ EPWPK  GFAKVDL +GE+KK++YGD RY
Sbjct: 470 SNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRY 529

Query: 516 GGEPYFLGREVYSDCEREDDGFIMVF 540
           GGEP FL  E       ED+G+I+ F
Sbjct: 530 GGEPLFLPGE----GGEEDEGYILCF 549

BLAST of Carg19945 vs. ExPASy TrEMBL
Match: A0A6J1EN25 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111434081 PE=3 SV=1)

HSP 1 Score: 1119.8 bits (2895), Expect = 0.0e+00
Identity = 530/538 (98.51%), Postives = 533/538 (99.07%), Query Frame = 0

Query: 1   MAMALSSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60
           MAMALSSSS S HLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE
Sbjct: 1   MAMALSSSSASLHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE 60

Query: 61  DDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120
           DDA+KCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS
Sbjct: 61  DDASKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYAPVPEQS 120

Query: 121 VSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFT 180
           VSHSLPVTGAIPDCI+GVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGC  YACRFT
Sbjct: 121 VSHSLPVTGAIPDCINGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCTSYACRFT 180

Query: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240
           ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF
Sbjct: 181 ETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYF 240

Query: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNF 300
           NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDP SGELFALSYNF
Sbjct: 241 NGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPDSGELFALSYNF 300

Query: 301 SHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360
           +HKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS
Sbjct: 301 THKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGS 360

Query: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420
           PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP
Sbjct: 361 PVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTP 420

Query: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480
           PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY
Sbjct: 421 PDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAY 480

Query: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMV 539
           LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMV
Sbjct: 481 LAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMV 538

BLAST of Carg19945 vs. ExPASy TrEMBL
Match: A0A6J1KII7 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111495550 PE=3 SV=1)

HSP 1 Score: 1092.0 bits (2823), Expect = 0.0e+00
Identity = 520/552 (94.20%), Postives = 528/552 (95.65%), Query Frame = 0

Query: 1   MAMALSSSSGSPH--------------LGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVF 60
           MAMALSSSS SPH              +G GLGF TRPIRKKPNLRNEVI+C LNSPSVF
Sbjct: 1   MAMALSSSSASPHFPFDRRLLEISTVAMGFGLGFLTRPIRKKPNLRNEVIACALNSPSVF 60

Query: 61  HYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPH 120
           HYPKQPNKRPRITEDDA+KCRH PPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPH
Sbjct: 61  HYPKQPNKRPRITEDDASKCRHRPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPH 120

Query: 121 VQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAV 180
           VQIAGNYAPVPEQSVSHSLPVTGAIPDCI+GVYLRNGANPLF+PLGGHHLFDGDGMIHAV
Sbjct: 121 VQIAGNYAPVPEQSVSHSLPVTGAIPDCINGVYLRNGANPLFDPLGGHHLFDGDGMIHAV 180

Query: 181 TIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDH 240
           TIGGGCA YACRFTETERLVQERSIGRP+FPKTIGELHGHSGIARLLLFYARGLFGLIDH
Sbjct: 181 TIGGGCASYACRFTETERLVQERSIGRPIFPKTIGELHGHSGIARLLLFYARGLFGLIDH 240

Query: 241 RHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKI 300
           RHGTGVANAGLVYFNG LLAMSEEDLPYHVRITPSGDLVTAGRYNFEG LDSPMVAHPKI
Sbjct: 241 RHGTGVANAGLVYFNGRLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGHLDSPMVAHPKI 300

Query: 301 DPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ 360
           DP SGELFALSYNF+HKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ
Sbjct: 301 DPDSGELFALSYNFTHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQ 360

Query: 361 QVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET 420
           QVVFNLRKMINGGSPVMYD+KKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET
Sbjct: 361 QVVFNLRKMINGGSPVMYDEKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEET 420

Query: 421 ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGM 480
           ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSK EEVNLEVGM
Sbjct: 421 ESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKSEEVNLEVGM 480

Query: 481 VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS 539
           VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS
Sbjct: 481 VNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYS 540

BLAST of Carg19945 vs. ExPASy TrEMBL
Match: A0A5A7UHG6 (Putative 9-cis-epoxycarotenoid dioxygenase NCED5 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold318G00330 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.9e-264
Identity = 438/551 (79.49%), Postives = 475/551 (86.21%), Query Frame = 0

Query: 3   MALSSSSGSPH-----------LGLGLGFPTRPI---RKKPNLRNEVISCTLNSPSVFHY 62
           M  SSSS SPH           LGLGLGFP  PI   +KKPN R EVIS  LNSP VFH+
Sbjct: 1   MVFSSSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHF 60

Query: 63  PKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQ 122
           PKQ NK PRITE   +KCR HP  WNLLQK AS AFDILEQ  LN DN NLPKNFDPH Q
Sbjct: 61  PKQTNKPPRITEHHTSKCR-HPSHWNLLQKVASTAFDILEQTFLNNDNQNLPKNFDPHFQ 120

Query: 123 IAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTI 182
           I GNYAPVPEQ VSHSLPVTG IPD I+GVYLRNGANP F+PL GHHLFDGDGMIHAVTI
Sbjct: 121 IVGNYAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTI 180

Query: 183 GGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRH 242
           GGG A YACRFTETERLVQE+SIGRPVFPK +GELHGHSGIARLLLFYARG FGLIDHR 
Sbjct: 181 GGGRASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRR 240

Query: 243 GTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDP 302
           GTGVANAGLVYFNG LLAMSE+DLPY VRITPSGDL+T GRYNFEGQL+SPMVAHPKIDP
Sbjct: 241 GTGVANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDP 300

Query: 303 VSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQV 362
            SGELFALSYNF+HKPYL HFR +PDGKMSPKI+IP++IPTM+HDFAITE FVVIPDQQV
Sbjct: 301 ESGELFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQV 360

Query: 363 VFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETES 422
           VFNL+KM+ GGSPVMYD+KK PRFGFLPKNATDSSNL WVDSPADTFCFHLWNAWEETES
Sbjct: 361 VFNLQKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETES 420

Query: 423 NEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVN 482
           ++VVVI SCMTPPDSIFN +DE+ KA+LTEIRFNL TG S+ RPIVS+ E +NLEVG+VN
Sbjct: 421 DDVVVIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVN 480

Query: 483 QSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDC 540
           +  +GRKT+Y YLAIT+PWPKACGFAKVD+ SGE+KKYIYGD+RYGGEPYFLGRE+   C
Sbjct: 481 RKRLGRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGC 540

BLAST of Carg19945 vs. ExPASy TrEMBL
Match: A0A1S3BG38 (probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103489449 PE=3 SV=1)

HSP 1 Score: 919.5 bits (2375), Expect = 6.9e-264
Identity = 438/551 (79.49%), Postives = 475/551 (86.21%), Query Frame = 0

Query: 3   MALSSSSGSPH-----------LGLGLGFPTRPI---RKKPNLRNEVISCTLNSPSVFHY 62
           M  SSSS SPH           LGLGLGFP  PI   +KKPN R EVIS  LNSP VFH+
Sbjct: 1   MVFSSSSTSPHFVLEQKGLGLGLGLGLGFPKNPINIVKKKPNQRKEVISFALNSPFVFHF 60

Query: 63  PKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQ 122
           PKQ NK PRITE   +KCR HP  WNLLQK AS AFDILEQ  LN DN NLPKNFDPH Q
Sbjct: 61  PKQTNKPPRITEHHTSKCR-HPSHWNLLQKVASTAFDILEQTFLNNDNQNLPKNFDPHFQ 120

Query: 123 IAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTI 182
           I GNYAPVPEQ VSHSLPVTG IPD I+GVYLRNGANP F+PL GHHLFDGDGMIHAVTI
Sbjct: 121 IVGNYAPVPEQPVSHSLPVTGVIPDWINGVYLRNGANPYFQPLAGHHLFDGDGMIHAVTI 180

Query: 183 GGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRH 242
           GGG A YACRFTETERLVQE+SIGRPVFPK +GELHGHSGIARLLLFYARG FGLIDHR 
Sbjct: 181 GGGRASYACRFTETERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGFFGLIDHRR 240

Query: 243 GTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDP 302
           GTGVANAGLVYFNG LLAMSE+DLPY VRITPSGDL+T GRYNFEGQL+SPMVAHPKIDP
Sbjct: 241 GTGVANAGLVYFNGRLLAMSEDDLPYQVRITPSGDLITVGRYNFEGQLNSPMVAHPKIDP 300

Query: 303 VSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQV 362
            SGELFALSYNF+HKPYL HFR +PDGKMSPKI+IP++IPTM+HDFAITE FVVIPDQQV
Sbjct: 301 ESGELFALSYNFTHKPYLKHFRLTPDGKMSPKIDIPLDIPTMIHDFAITEKFVVIPDQQV 360

Query: 363 VFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETES 422
           VFNL+KM+ GGSPVMYD+KK PRFGFLPKNATDSSNL WVDSPADTFCFHLWNAWEETES
Sbjct: 361 VFNLQKMLTGGSPVMYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETES 420

Query: 423 NEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVN 482
           ++VVVI SCMTPPDSIFN +DE+ KA+LTEIRFNL TG S+ RPIVS+ E +NLEVG+VN
Sbjct: 421 DDVVVIASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRPIVSESEHMNLEVGVVN 480

Query: 483 QSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDC 540
           +  +GRKT+Y YLAIT+PWPKACGFAKVD+ SGE+KKYIYGD+RYGGEPYFLGRE+   C
Sbjct: 481 RKRLGRKTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGC 540

BLAST of Carg19945 vs. ExPASy TrEMBL
Match: A0A0A0M030 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G435760 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 2.1e-257
Identity = 431/545 (79.08%), Postives = 471/545 (86.42%), Query Frame = 0

Query: 3   MALSSSSGSPHLGL---GLGFPTRPI---RKKPNLRNEVISCTL--NSPSVFHYPKQPNK 62
           M  SS+S SPH  L   GLGFP +PI   +KKPN R EVIS  L  NSP VFH+PKQPNK
Sbjct: 1   MVFSSTSTSPHFVLDQKGLGFPIKPINIVKKKPNQRKEVISSALKVNSPFVFHFPKQPNK 60

Query: 63  RPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYDNLNLPKNFDPHVQIAGNYA 122
            PRITE    K R HP  WNLLQK AS AFDILEQA LN  N NLPKNFDPH QI GNYA
Sbjct: 61  GPRITEHYTFKSR-HPSHWNLLQKVASTAFDILEQAFLNNHNRNLPKNFDPHFQIVGNYA 120

Query: 123 PVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCAR 182
           PVPEQ VSHSLPVTG IP  I+GVYLRNGANP F+PL GHHLFDGDGMIHAVTIG G A 
Sbjct: 121 PVPEQPVSHSLPVTGVIPHWINGVYLRNGANPFFQPLSGHHLFDGDGMIHAVTIGEGRAS 180

Query: 183 YACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVAN 242
           YACRFT+TERLVQE+SIGRPVFPK +GELHGHSGIARLLLFYARGLFGLIDHR GTGVAN
Sbjct: 181 YACRFTQTERLVQEKSIGRPVFPKAVGELHGHSGIARLLLFYARGLFGLIDHRRGTGVAN 240

Query: 243 AGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELF 302
           AGLVYFNG LLAMSE+DLPY VRITPSGDL+T GRYNFEGQL+SPMVAHPKIDP SGELF
Sbjct: 241 AGLVYFNGRLLAMSEDDLPYQVRITPSGDLITIGRYNFEGQLNSPMVAHPKIDPDSGELF 300

Query: 303 ALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRK 362
           ALSYNF+HKPYL HFR +PDGKMSP+I+IP+EIPTM+HDFAITE FVVIPDQQVVFNL+K
Sbjct: 301 ALSYNFTHKPYLKHFRLTPDGKMSPEIDIPLEIPTMIHDFAITEKFVVIPDQQVVFNLQK 360

Query: 363 MINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVI 422
           M++GGSPV+YD+KK PRFGFLPKNATDSSNL WVDSPADTFCFHLWNAWEETES+++VVI
Sbjct: 361 MLSGGSPVVYDEKKNPRFGFLPKNATDSSNLTWVDSPADTFCFHLWNAWEETESDDIVVI 420

Query: 423 GSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGR 482
            SCMTPPDSIFN +DE+ KA+LTEIRFNL TG S+ R I+S+ E VNLEVG+VN+  VGR
Sbjct: 421 ASCMTPPDSIFNERDEEFKAVLTEIRFNLRTGKSSSRRIISESEHVNLEVGVVNRKRVGR 480

Query: 483 KTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDG 540
           KT+Y YLAIT+PWPKACGFAKVD+ SGE+KKYIYGD+RYGGEPYFLGRE+   CE EDDG
Sbjct: 481 KTQYVYLAITDPWPKACGFAKVDVSSGEVKKYIYGDERYGGEPYFLGREMGFGCEEEDDG 540

BLAST of Carg19945 vs. TAIR 10
Match: AT1G30100.1 (nine-cis-epoxycarotenoid dioxygenase 5 )

HSP 1 Score: 690.3 bits (1780), Expect = 1.3e-198
Identity = 332/521 (63.72%), Postives = 397/521 (76.20%), Query Frame = 0

Query: 28  KPNLRNEVISCTLNSPSVFHYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDIL 87
           KP  R    +   ++P++ ++P  P+  P I E D +       RWN LQ+ AS A D  
Sbjct: 38  KPRRRKLSANSVSDTPNLLNFPNYPSPNPIIPEKDTS-------RWNPLQRAASAALDFA 97

Query: 88  EQALLNYDNLN-LPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANP 147
           E ALL  +    LPK  DP  QI+GNYAPVPEQSV  SL V G IPDCIDGVYLRNGANP
Sbjct: 98  ETALLRRERSKPLPKTVDPRHQISGNYAPVPEQSVKSSLSVDGKIPDCIDGVYLRNGANP 157

Query: 148 LFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGH 207
           LFEP+ GHHLFDGDGM+HAV I  G A Y+CRFTETERLVQE+ +G P+FPK IGELHGH
Sbjct: 158 LFEPVSGHHLFDGDGMVHAVKITNGDASYSCRFTETERLVQEKQLGSPIFPKAIGELHGH 217

Query: 208 SGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVT 267
           SGIARL+LFYARGLFGL++H++GTGVANAGLVYF+  LLAMSE+DLPY VR+T +GDL T
Sbjct: 218 SGIARLMLFYARGLFGLLNHKNGTGVANAGLVYFHDRLLAMSEDDLPYQVRVTDNGDLET 277

Query: 268 AGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVE 327
            GR++F+GQL S M+AHPKIDPV+ ELFALSY+   KPYL +F+FSP+G+ SP +EIP+ 
Sbjct: 278 IGRFDFDGQLSSAMIAHPKIDPVTKELFALSYDVVKKPYLKYFKFSPEGEKSPDVEIPLA 337

Query: 328 IPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLI 387
            PTMMHDFAITENFVVIPDQQVVF L  M  G SPV YD +K  RFG LP+NA D+S ++
Sbjct: 338 SPTMMHDFAITENFVVIPDQQVVFKLSDMFLGKSPVKYDGEKISRFGILPRNAKDASEMV 397

Query: 388 WVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTG 447
           WV+SP +TFCFHLWNAWE  E++EVVVIGSCMTP DSIFN  DE L ++L+EIR NL TG
Sbjct: 398 WVESP-ETFCFHLWNAWESPETDEVVVIGSCMTPADSIFNECDEQLNSVLSEIRLNLKTG 457

Query: 448 DSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKY 507
            S RR I+    ++NLE GMVN++L+GRKTRYAYLAI EPWPK  GFAKVDL +GE+K +
Sbjct: 458 KSTRRTIIPGSVQMNLEAGMVNRNLLGRKTRYAYLAIAEPWPKVSGFAKVDLSTGEVKNH 517

Query: 508 IYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFGARREEME 548
            YG ++YGGEP+FL R + SD   EDDG+IM F    E  E
Sbjct: 518 FYGGKKYGGEPFFLPRGLESD--GEDDGYIMSFVHDEESWE 548

BLAST of Carg19945 vs. TAIR 10
Match: AT3G14440.1 (nine-cis-epoxycarotenoid dioxygenase 3 )

HSP 1 Score: 677.9 bits (1748), Expect = 6.7e-195
Identity = 321/506 (63.44%), Postives = 392/506 (77.47%), Query Frame = 0

Query: 36  ISCTLNSPSVFHYPKQPNKRPRITEDDATKCRHHPPRWNLLQKGASMAFDILEQALLNYD 95
           +S  L++P   H+PKQ +  P I      K   +  + NL Q+ A+ A D  E  L++++
Sbjct: 50  VSSALHTPPALHFPKQSSNSPAIVVKPKAK-ESNTKQMNLFQRAAAAALDAAEGFLVSHE 109

Query: 96  NLN-LPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGH 155
            L+ LPK  DP VQIAGN+APV EQ V  +LPV G +PD I GVY+RNGANPL EP+ GH
Sbjct: 110 KLHPLPKTADPSVQIAGNFAPVNEQPVRRNLPVVGKLPDSIKGVYVRNGANPLHEPVTGH 169

Query: 156 HLFDGDGMIHAVTIGGGCARYACRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLL 215
           H FDGDGM+HAV    G A YACRFT+T R VQER +GRPVFPK IGELHGH+GIARL+L
Sbjct: 170 HFFDGDGMVHAVKFEHGSASYACRFTQTNRFVQERQLGRPVFPKAIGELHGHTGIARLML 229

Query: 216 FYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEG 275
           FYAR   G++D  HGTGVANAGLVYFNG LLAMSE+DLPY V+ITP+GDL T GR++F+G
Sbjct: 230 FYARAAAGIVDPAHGTGVANAGLVYFNGRLLAMSEDDLPYQVQITPNGDLKTVGRFDFDG 289

Query: 276 QLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDF 335
           QL+S M+AHPK+DP SGELFALSY+   KPYL +FRFSPDG  SP +EI ++ PTMMHDF
Sbjct: 290 QLESTMIAHPKVDPESGELFALSYDVVSKPYLKYFRFSPDGTKSPDVEIQLDQPTMMHDF 349

Query: 336 AITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADT 395
           AITENFVV+PDQQVVF L +MI GGSPV+YD  K  RFG L K A DSSN+ W+D+P D 
Sbjct: 350 AITENFVVVPDQQVVFKLPEMIRGGSPVVYDKNKVARFGILDKYAEDSSNIKWIDAP-DC 409

Query: 396 FCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIV 455
           FCFHLWNAWEE E++EVVVIGSCMTPPDSIFN  DE+LK++L+EIR NL TG+S RRPI+
Sbjct: 410 FCFHLWNAWEEPETDEVVVIGSCMTPPDSIFNESDENLKSVLSEIRLNLKTGESTRRPII 469

Query: 456 S-KPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRY 515
           S + ++VNLE GMVN++++GRKT++AYLA+ EPWPK  GFAKVDL +GE+KK++YGD RY
Sbjct: 470 SNEDQQVNLEAGMVNRNMLGRKTKFAYLALAEPWPKVSGFAKVDLTTGEVKKHLYGDNRY 529

Query: 516 GGEPYFLGREVYSDCEREDDGFIMVF 540
           GGEP FL  E       ED+G+I+ F
Sbjct: 530 GGEPLFLPGE----GGEEDEGYILCF 549

BLAST of Carg19945 vs. TAIR 10
Match: AT4G18350.1 (nine-cis-epoxycarotenoid dioxygenase 2 )

HSP 1 Score: 674.1 bits (1738), Expect = 9.7e-194
Identity = 326/526 (61.98%), Postives = 409/526 (77.76%), Query Frame = 0

Query: 12  PHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITEDDATKCRHHPP 71
           P   + LGF  RPI+++P    +VI CT+         K+    PR T   AT  +H+P 
Sbjct: 17  PQAQIDLGF--RPIKRQP----KVIKCTVQIDVTELTKKRQLFTPRTT---ATPPQHNPL 76

Query: 72  RWNLLQKGASMAFDILEQALLNYD-NLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVTGA 131
           R N+ QK A++A D  E+AL++++ +  LPK  DP VQIAGNY+PVPE SV  +L V G 
Sbjct: 77  RLNIFQKAAAIAIDAAERALISHEQDSPLPKTADPRVQIAGNYSPVPESSVRRNLTVEGT 136

Query: 132 IPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYACRFTETERLVQERS 191
           IPDCIDGVY+RNGANP+FEP  GHHLFDGDGM+HAV I  G A YACRFT+TERLVQE+ 
Sbjct: 137 IPDCIDGVYIRNGANPMFEPTAGHHLFDGDGMVHAVKITNGSASYACRFTKTERLVQEKR 196

Query: 192 IGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAMSEE 251
           +GRPVFPK IGELHGHSGIARL+LFYARGL GLI++++G GVANAGLVYFN  LLAMSE+
Sbjct: 197 LGRPVFPKAIGELHGHSGIARLMLFYARGLCGLINNQNGVGVANAGLVYFNNRLLAMSED 256

Query: 252 DLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLTHFR 311
           DLPY ++IT +GDL T GRY+F+GQL S M+AHPK+DPV+ EL ALSY+   KPYL +FR
Sbjct: 257 DLPYQLKITQTGDLQTVGRYDFDGQLKSAMIAHPKLDPVTKELHALSYDVVKKPYLKYFR 316

Query: 312 FSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDKKTP 371
           FSPDG  SP++EIP+E PTM+HDFAITENFVVIPDQQVVF L +MI+G SPV++D +K  
Sbjct: 317 FSPDGVKSPELEIPLETPTMIHDFAITENFVVIPDQQVVFKLGEMISGKSPVVFDGEKVS 376

Query: 372 RFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGSCMTPPDSIFNGQDE 431
           R G +PK+AT++S +IWV+SP +TFCFHLWNAWE  E+ E+VVIGSCM+P DSIFN +DE
Sbjct: 377 RLGIMPKDATEASQIIWVNSP-ETFCFHLWNAWESPETEEIVVIGSCMSPADSIFNERDE 436

Query: 432 DLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAITEPWPKA 491
            L+++L+EIR NL T  + RR ++   E+VNLE+GMVN++ +GRKTR+A+LAI  PWPK 
Sbjct: 437 SLRSVLSEIRINLRTRKTTRRSLLVN-EDVNLEIGMVNRNRLGRKTRFAFLAIAYPWPKV 496

Query: 492 CGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFI 537
            GFAKVDL +GE+KKYIYG ++YGGEP+FL     +  E EDDG+I
Sbjct: 497 SGFAKVDLCTGEMKKYIYGGEKYGGEPFFLPGNSGNGEENEDDGYI 531

BLAST of Carg19945 vs. TAIR 10
Match: AT1G78390.1 (nine-cis-epoxycarotenoid dioxygenase 9 )

HSP 1 Score: 667.2 bits (1720), Expect = 1.2e-191
Identity = 332/551 (60.25%), Postives = 409/551 (74.23%), Query Frame = 0

Query: 6   SSSSGSPHLGLGLGFPTRPIRKKPNLRNEVISCTLNSPSVFHYPKQPNKRPRITE---DD 65
           SSSS  P L   L F +    KK  +   V S      SV    + P  +P   +   +D
Sbjct: 72  SSSSSRPKL-QSLSFSSTLRNKKLVVPCYVSSSVNKKSSVSSSLQSPTFKPPSWKKLCND 131

Query: 66  AT----KCRHHPPRWNLLQKGASMAFDILEQALLNYDNLN--LPKNFDPHVQIAGNYAPV 125
            T    K  +  P+ N +Q+ A+M  D +E A+++++      PK  DP VQIAGN+ PV
Sbjct: 132 VTNLIPKTTNQNPKLNPVQRTAAMVLDAVENAMISHERRRHPHPKTADPAVQIAGNFFPV 191

Query: 126 PEQSVSHSLPVTGAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIGGGCARYA 185
           PE+ V H+LPVTG +P+CI GVY+RNGANPL +P+ GHHLFDGDGM+HAV    G   YA
Sbjct: 192 PEKPVVHNLPVTGTVPECIQGVYVRNGANPLHKPVSGHHLFDGDGMVHAVRFDNGSVSYA 251

Query: 186 CRFTETERLVQERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAG 245
           CRFTET RLVQER  GRPVFPK IGELHGH GIA+L+LF  RGLFGL+D   G GVANAG
Sbjct: 252 CRFTETNRLVQERECGRPVFPKAIGELHGHLGIAKLMLFNTRGLFGLVDPTGGLGVANAG 311

Query: 246 LVYFNGHLLAMSEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFAL 305
           LVYFNGHLLAMSE+DLPYHV++T +GDL T+GRY+F+GQL S M+AHPKIDP + ELFAL
Sbjct: 312 LVYFNGHLLAMSEDDLPYHVKVTQTGDLETSGRYDFDGQLKSTMIAHPKIDPETRELFAL 371

Query: 306 SYNFSHKPYLTHFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMI 365
           SY+   KPYL +FRF+ DG+ SP +EIP++ PTM+HDFAITENFVVIPDQQVVF L +MI
Sbjct: 372 SYDVVSKPYLKYFRFTSDGEKSPDVEIPLDQPTMIHDFAITENFVVIPDQQVVFRLPEMI 431

Query: 366 NGGSPVMYDDKKTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEETESNEVVVIGS 425
            GGSPV+YD+KK  RFG L KNA D+S++ W++ P D FCFHLWN+WEE E++EVVVIGS
Sbjct: 432 RGGSPVVYDEKKKSRFGILNKNAKDASSIQWIEVP-DCFCFHLWNSWEEPETDEVVVIGS 491

Query: 426 CMTPPDSIFNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKT 485
           CMTPPDSIFN  DE L+++L+EIR NL TG+S RRP++S  E+VNLE GMVN++L+GRKT
Sbjct: 492 CMTPPDSIFNEHDETLQSVLSEIRLNLKTGESTRRPVIS--EQVNLEAGMVNRNLLGRKT 551

Query: 486 RYAYLAITEPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFI 545
           RYAYLA+TEPWPK  GFAKVDL +GEI+KYIYG+ +YGGEP FL     S    ED G+I
Sbjct: 552 RYAYLALTEPWPKVSGFAKVDLSTGEIRKYIYGEGKYGGEPLFLP----SGDGEEDGGYI 611

Query: 546 MVFGARREEME 548
           MVF    E+++
Sbjct: 612 MVFVHDEEKVK 614

BLAST of Carg19945 vs. TAIR 10
Match: AT3G24220.1 (nine-cis-epoxycarotenoid dioxygenase 6 )

HSP 1 Score: 525.0 bits (1351), Expect = 7.3e-149
Identity = 264/483 (54.66%), Postives = 337/483 (69.77%), Query Frame = 0

Query: 71  PRWNLLQKGASMAFDILEQALL--NYDNLNLPKNFDPHVQIAGNYAPVPEQSVSHSLPVT 130
           P  NLLQK A+   D +E +++     N  LPK  DP VQ++GN+APV E  V + L V 
Sbjct: 61  PNLNLLQKLAATMLDKIESSIVIPMEQNRPLPKPTDPAVQLSGNFAPVNECPVQNGLEVV 120

Query: 131 GAIPDCIDGVYLRNGANPLFEPLGGHHLFDGDGMIHAVTIG-GGCARYACRFTETERLVQ 190
           G IP C+ GVY+RNGANP+F PL GHHLFDGDGMIHAV+IG      Y+CR+T+T RLVQ
Sbjct: 121 GQIPSCLKGVYIRNGANPMFPPLAGHHLFDGDGMIHAVSIGFDNQVSYSCRYTKTNRLVQ 180

Query: 191 ERSIGRPVFPKTIGELHGHSGIARLLLFYARGLFGLIDHRHGTGVANAGLVYFNGHLLAM 250
           E ++GR VFPK IGELHGHSG+ARL LF AR   GL+D   G GVANAG+V+FNG LLAM
Sbjct: 181 ETALGRSVFPKPIGELHGHSGLARLALFTARAGIGLVDGTRGMGVANAGVVFFNGRLLAM 240

Query: 251 SEEDLPYHVRITPSGDLVTAGRYNFEGQLDSPMVAHPKIDPVSGELFALSYNFSHKPYLT 310
           SE+DLPY V+I   GDL T GR+ F+ Q+DS ++AHPK+D  +G+L  LSYN   KP+L 
Sbjct: 241 SEDDLPYQVKIDGQGDLETIGRFGFDDQIDSSVIAHPKVDATTGDLHTLSYNVLKKPHLR 300

Query: 311 HFRFSPDGKMSPKIEIPVEIPTMMHDFAITENFVVIPDQQVVFNLRKMINGGSPVMYDDK 370
           + +F+  GK +  +EI +  PTM+HDFAITENFVVIPDQQ+VF L +MI GGSPV+Y  +
Sbjct: 301 YLKFNTCGKKTRDVEITLPEPTMIHDFAITENFVVIPDQQMVFKLSEMIRGGSPVIYVKE 360

Query: 371 KTPRFGFLPKNATDSSNLIWVDSPADTFCFHLWNAWEE-TESNE--VVVIGSCMTPPDSI 430
           K  RFG L K     S++ WVD P D FCFHLWNAWEE TE  +  +VVIGSCM+PPD+I
Sbjct: 361 KMARFGVLSKQDLTGSDINWVDVP-DCFCFHLWNAWEERTEEGDPVIVVIGSCMSPPDTI 420

Query: 431 FNGQDEDLKAILTEIRFNLVTGDSARRPIVSKPEEVNLEVGMVNQSLVGRKTRYAYLAIT 490
           F+   E  +  L+EIR N+ T +S R+ IV+    VNLE G +N+S VGRK+++ Y+AI 
Sbjct: 421 FSESGEPTRVELSEIRLNMRTKESNRKVIVT---GVNLEAGHINRSYVGRKSQFVYIAIA 480

Query: 491 EPWPKACGFAKVDLWSGEIKKYIYGDQRYGGEPYFLGREVYSDCEREDDGFIMVFGARRE 548
           +PWPK  G AKVD+ +G + ++ YG  R+GGEP F    V      ED G++M F  R E
Sbjct: 481 DPWPKCSGIAKVDIQNGTVSEFNYGPSRFGGEPCF----VPEGEGEEDKGYVMGF-VRDE 534

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019511.10.0e+00100.00putative 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic, partial [Cucurb... [more]
XP_022927145.10.0e+0098.51probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita mosch... [more]
XP_023520051.10.0e+0097.22probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita pepo ... [more]
XP_023001406.10.0e+0094.20probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic [Cucurbita maxim... [more]
KAG6583893.11.0e-27799.789-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic, partial [Cucurbita argyr... [more]
Match NameE-valueIdentityDescription
K4CJJ15.2e-20865.549-cis-epoxycarotenoid dioxygenase NCED2, chloroplastic OS=Solanum lycopersicum O... [more]
O240233.3e-19963.059-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Solanum lycopersicum O... [more]
Q9C6Z11.8e-19763.72Probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Arabidopsis t... [more]
Q9M6E85.3e-19759.869-cis-epoxycarotenoid dioxygenase NCED1, chloroplastic OS=Phaseolus vulgaris OX=... [more]
Q9LRR79.4e-19463.449-cis-epoxycarotenoid dioxygenase NCED3, chloroplastic OS=Arabidopsis thaliana O... [more]
Match NameE-valueIdentityDescription
A0A6J1EN250.0e+0098.51probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita mos... [more]
A0A6J1KII70.0e+0094.20probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucurbita max... [more]
A0A5A7UHG66.9e-26479.49Putative 9-cis-epoxycarotenoid dioxygenase NCED5 OS=Cucumis melo var. makuwa OX=... [more]
A0A1S3BG386.9e-26479.49probable 9-cis-epoxycarotenoid dioxygenase NCED5, chloroplastic OS=Cucumis melo ... [more]
A0A0A0M0302.1e-25779.08Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_1G435760 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30100.11.3e-19863.72nine-cis-epoxycarotenoid dioxygenase 5 [more]
AT3G14440.16.7e-19563.44nine-cis-epoxycarotenoid dioxygenase 3 [more]
AT4G18350.19.7e-19461.98nine-cis-epoxycarotenoid dioxygenase 2 [more]
AT1G78390.11.2e-19160.25nine-cis-epoxycarotenoid dioxygenase 9 [more]
AT3G24220.17.3e-14954.66nine-cis-epoxycarotenoid dioxygenase 6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004294Carotenoid oxygenasePFAMPF03055RPE65coord: 115..542
e-value: 7.0E-89
score: 299.1
IPR004294Carotenoid oxygenasePANTHERPTHR10543BETA-CAROTENE DIOXYGENASEcoord: 36..540
NoneNo IPR availablePANTHERPTHR10543:SF1239-CIS-EPOXYCAROTENOID DIOXYGENASE NCED5, CHLOROPLASTIC-RELATEDcoord: 36..540

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19945-RACarg19945-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016121 carotene catabolic process
cellular_component GO:0009570 chloroplast stroma
molecular_function GO:0010436 carotenoid dioxygenase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0016702 oxidoreductase activity, acting on single donors with incorporation of molecular oxygen, incorporation of two atoms of oxygen