Carg19939 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19939
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCalcyclin-binding protein
LocationCarg_Chr13: 6729201 .. 6731374 (+)
RNA-Seq ExpressionCarg19939
SyntenyCarg19939
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGGAAGACTTGTCTCTTGATTTGGAGGAGCTTCGCCACCTCCACAGCATTGCCAAGAGGCCTCGAGTCGTCTCTCTCATTTCCTCTGAGATTAGCAACTTAGAGAAGGTTCCTCTCTGTTTTCTCTTATTGATTTGGATTAGTTTGTGCCATTTTAATGATCTTATTGATACGTTTTTTTGAAGAAATTTTAAAATTATCGTTATCTAATGGTTTGTCATGCTGTATAACCTAGTTTACGGACTGAATTTGATATTTCGTTAGGGGTTATCTTCCGTCGCTTTGAGATATATGAATCTATTACCTCAATTATTGATACATCGCTTCAATCGATTTATTCTACTACTGTATTAGGCTTCAGCATTTTCTTGTCTTACGAGAATTATGTTGCTATGTTCACTCAGTTGTCAAAAGAGACTGTCCCACAAACTCACGCTTCCACTCCTGCTTCAATTTCAACTGCTAAGGTGTCCATCAATTCAGGCATTGTTTACACTCCACTACCTGGATTCAGCTGGGATCAGGACAATGACAAAGTGAAGGTAACGAACTTTAAGCCTCCACATCATGGCACAGCTAGATCCCATTGGCGAAAATTAGAGCATGTTAAGATTTTAAGCCTCATCCTGATGGATGTCATTATCATTGTCATGTAGATATATGTGTCGCTTGAAGGAATAGAGGATGAAAAAGTTGAGGCTGACTACAAGAAACTATCGATCGACATCAAGTTCCATGATGTCAAAGGGAAGAATTACCGGTTTGCAATTCCTAAACTGAACAAGGAAATTGTTCCTGAGAAGTGCAAATTGCTAGTGAAACCAACACGGGCTGTGATCACGTTGTACAAAGCTTCAAAAGGGAACTGGTTAGACTTGCATTTGAAGGAGGAGAAAGTGAGTGATTTAAGAGAGTAATTGGTGATTTAGTTGTATCTTAGAGGTATTTATTCAACTAACTTTGTTGTTATTTACATCTTACAGCTCAAGCCAGGTCTTGATAAGGAACGCGACCCAATGGCAGGAATCATGGATTTAATGAAGGTAACATCTTCTTAGCCTATGCATGGTTTGGAAAGGGAGGAATGTTTCTCTTTCTTTCCTATTCTTTGTTTTGTTGGGTGTTGGTGGTCATAAAATTTTCTTCCATATTTTTGCCTCATAGTCGTAGTTTATCTGCTTTGGTTGTTAGCAACTATTAGTTATGGTGAGAATAAAAAGTGGATAATGATGAGGATATGATGTGTTCTTTCAAGTGATAATGATGAGCTGGAAGTAGATAAAGTTAGGTTAAAATCCTATGCTTGTCATCTACTTAGGAGTACGAGTTTCTTGACAACTTAGTGGTGGGTCATATTATTGACTGCCCTGTGAATTAATCAGGTGCATGTTTCTAGATTTTTAAGTGTTGGGTCACATGGTTGAGATTGGTTGTTAGATTAGTTTGAGATACGGACTTTGTCGGCATGAACACTTTGAAATAAAAAAGTAGTACCACGGCTGACATATATCTATGGAGGATAAGAAACCCCCATCTCGACAATTGGGCTGAAAAGGAGTCCATGAATCTGAATTGTTACATCAAGCCAGCCTAACGAACTGATTCTTGATCTTATCGCTGCTCTCCGTTGGTGTTTTGAACAAATTCAAATCCAAACTTTTGAATTGATTTTCAACTCATTACAGTATATGGGTATCTGTTCTGCTCTGTGTGAGATAGGTACACACGCCTCTGACAATGTTATATACAATTAGGATAAGCATAAAACTGCATCTGTATTTAGGCTGAACACACTGATCTCTGCGATTGCTGATTTATTATGGCTGACATGGAAACTGCAGAACATGTACGACGAAGGAGATGATGAAATGAAAAGAACGATAGCAAAGGCATGGACTGATGCCAGATCCGGCAAGACAGCTGACCCACTTAAAGGGTTCCCATGAGCAAGCGCAGGGCAAATCTAGTTATCATCTTTTAGGAATGGTTCTCTGGTTGGATGAGATCCTATTTGTTTTGATGATGTTCAGTGGACCGCGTTTTATTGGCCTTCCTATTATGGTTTGTTTCCTTTAGATGGCGTTAGTTCTGAAGGAGTTTGTATAAAAACCTATGGATTTTGATATCTATAAAAAGTTCATATTTGACATATTAAAGTGGTGGCGGAACAA

mRNA sequence

ATGGCGGAAGACTTGTCTCTTGATTTGGAGGAGCTTCGCCACCTCCACAGCATTGCCAAGAGGCCTCGAGTCGTCTCTCTCATTTCCTCTGAGATTAGCAACTTAGAGAAGTTGTCAAAAGAGACTGTCCCACAAACTCACGCTTCCACTCCTGCTTCAATTTCAACTGCTAAGGTGTCCATCAATTCAGGCATTGTTTACACTCCACTACCTGGATTCAGCTGGGATCAGGACAATGACAAAGTGAAGATATATGTGTCGCTTGAAGGAATAGAGGATGAAAAAGTTGAGGCTGACTACAAGAAACTATCGATCGACATCAAGTTCCATGATGTCAAAGGGAAGAATTACCGGTTTGCAATTCCTAAACTGAACAAGGAAATTGTTCCTGAGAAGTGCAAATTGCTAGTGAAACCAACACGGGCTGTGATCACGTTGTACAAAGCTTCAAAAGGGAACTGGTTAGACTTGCATTTGAAGGAGGAGAAACTCAAGCCAGGTCTTGATAAGGAACGCGACCCAATGGCAGGAATCATGGATTTAATGAAGAACATGTACGACGAAGGAGATGATGAAATGAAAAGAACGATAGCAAAGGCATGGACTGATGCCAGATCCGGCAAGACAGCTGACCCACTTAAAGGGTTCCCATGAGCAAGCGCAGGGCAAATCTAGTTATCATCTTTTAGGAATGGTTCTCTGGTTGGATGAGATCCTATTTGTTTTGATGATGTTCAGTGGACCGCGTTTTATTGGCCTTCCTATTATGGTTTGTTTCCTTTAGATGGCGTTAGTTCTGAAGGAGTTTGTATAAAAACCTATGGATTTTGATATCTATAAAAAGTTCATATTTGACATATTAAAGTGGTGGCGGAACAA

Coding sequence (CDS)

ATGGCGGAAGACTTGTCTCTTGATTTGGAGGAGCTTCGCCACCTCCACAGCATTGCCAAGAGGCCTCGAGTCGTCTCTCTCATTTCCTCTGAGATTAGCAACTTAGAGAAGTTGTCAAAAGAGACTGTCCCACAAACTCACGCTTCCACTCCTGCTTCAATTTCAACTGCTAAGGTGTCCATCAATTCAGGCATTGTTTACACTCCACTACCTGGATTCAGCTGGGATCAGGACAATGACAAAGTGAAGATATATGTGTCGCTTGAAGGAATAGAGGATGAAAAAGTTGAGGCTGACTACAAGAAACTATCGATCGACATCAAGTTCCATGATGTCAAAGGGAAGAATTACCGGTTTGCAATTCCTAAACTGAACAAGGAAATTGTTCCTGAGAAGTGCAAATTGCTAGTGAAACCAACACGGGCTGTGATCACGTTGTACAAAGCTTCAAAAGGGAACTGGTTAGACTTGCATTTGAAGGAGGAGAAACTCAAGCCAGGTCTTGATAAGGAACGCGACCCAATGGCAGGAATCATGGATTTAATGAAGAACATGTACGACGAAGGAGATGATGAAATGAAAAGAACGATAGCAAAGGCATGGACTGATGCCAGATCCGGCAAGACAGCTGACCCACTTAAAGGGTTCCCATGA

Protein sequence

MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Homology
BLAST of Carg19939 vs. NCBI nr
Match: KAG7019517.1 (Calcyclin-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 429.5 bits (1103), Expect = 1.7e-116
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. NCBI nr
Match: KAG6583900.1 (Calcyclin-binding protein, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 426.0 bits (1094), Expect = 1.9e-115
Identity = 215/217 (99.08%), Postives = 217/217 (100.00%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIE+EKVEAD+KKLSIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEEEKVEADHKKLSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. NCBI nr
Match: XP_022927580.1 (calcyclin-binding protein [Cucurbita moschata])

HSP 1 Score: 423.7 bits (1088), Expect = 9.6e-115
Identity = 213/217 (98.16%), Postives = 216/217 (99.54%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRV+SLISSEISNLEKLSKETVPQTHASTPASISTAKVS
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVLSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDND+VKIYVSLEGIE EKVEADYKKLSIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDRVKIYVSLEGIEQEKVEADYKKLSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKE+KLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEDKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. NCBI nr
Match: XP_023001468.1 (calcyclin-binding protein [Cucurbita maxima])

HSP 1 Score: 417.2 bits (1071), Expect = 8.9e-113
Identity = 210/217 (96.77%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRV+SLISSEISNLEKLSKETVPQTHA TP SISTAKV 
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVLSLISSEISNLEKLSKETVPQTHAPTPVSISTAKVP 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIE EKVEADYKK SIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEQEKVEADYKKQSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKE+KLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEDKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. NCBI nr
Match: XP_023520274.1 (calcyclin-binding protein [Cucurbita pepo subsp. pepo])

HSP 1 Score: 416.4 bits (1069), Expect = 1.5e-112
Identity = 211/217 (97.24%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETV QTHAS P SISTAKV 
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVLQTHASIPVSISTAKVP 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIE EKVEADYKKLSIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEQEKVEADYKKLSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKE+KLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEDKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. ExPASy Swiss-Prot
Match: Q9CXW3 (Calcyclin-binding protein OS=Mus musculus OX=10090 GN=Cacybp PE=1 SV=1)

HSP 1 Score: 104.0 bits (258), Expect = 2.2e-21
Identity = 68/214 (31.78%), Postives = 110/214 (51.40%), Query Frame = 0

Query: 3   EDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVSIN 62
           E+L  DLEE++ L   + R R+   ++SE S +E   K  + Q     P   +    ++ 
Sbjct: 6   EELQKDLEEVKVLLEKSTRKRLRDTLTSEKSKIETELKNKMQQKSQKKPELDNEKPAAVV 65

Query: 63  SGIV--YT-PLPGFSWDQDNDKVKIYVSLEGIED---EKVEADYKKLSIDIKFHDVKGKN 122
           + +   YT  +  + WDQ +  VKIY++L G+     E V+  + + S D+   ++ GKN
Sbjct: 66  APLTTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLNGKN 125

Query: 123 YRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLK-----EEKLKPGLDKE 182
           Y   +  L K I  E     VK    +I   K ++    D   +     +EK KP  D E
Sbjct: 126 YSMIVNNLLKPISVESSSKKVKTDTVIILCRKKAENTRWDYLTQVEKECKEKEKPSYDTE 185

Query: 183 RDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDAR 206
            DP  G+M+++K +Y++GDD+MKRTI KAW ++R
Sbjct: 186 ADPSEGLMNVLKKIYEDGDDDMKRTINKAWVESR 219

BLAST of Carg19939 vs. ExPASy Swiss-Prot
Match: Q3T168 (Calcyclin-binding protein OS=Bos taurus OX=9913 GN=CACYBP PE=2 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 73/219 (33.33%), Postives = 111/219 (50.68%), Query Frame = 0

Query: 3   EDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQ--------THASTPASI 62
           E+L  DLEE++ L   A R RV   +++E S +E   K  + Q        T    PA++
Sbjct: 6   EELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEMKNKMQQKSQRKAELTENEKPAAV 65

Query: 63  STAKVSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIED---EKVEADYKKLSIDIKFHD 122
                 I +G     +  + WDQ +  VKIY++L G+     E V+ ++ + S D+   +
Sbjct: 66  ---VAPITTGYT-VKISNYGWDQSDKFVKIYITLPGVHQVPAESVQVNFTERSFDLLVKN 125

Query: 123 VKGKNYRFAIPKLNKEIVPEKCKLLVK-PTRAVITLYKASKGNWLDLHLKE----EKLKP 182
           + GK+Y   +  L K I  E     VK  T  ++   KA    W  L   E    EK KP
Sbjct: 126 LNGKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKAENTRWDYLTQVEKECKEKEKP 185

Query: 183 GLDKERDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDAR 206
             D E DP  G+M+++K +Y++GDD+MKRTI KAW ++R
Sbjct: 186 SYDTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESR 220

BLAST of Carg19939 vs. ExPASy Swiss-Prot
Match: Q6AYK6 (Calcyclin-binding protein OS=Rattus norvegicus OX=10116 GN=Cacybp PE=1 SV=1)

HSP 1 Score: 101.7 bits (252), Expect = 1.1e-20
Identity = 66/214 (30.84%), Postives = 110/214 (51.40%), Query Frame = 0

Query: 3   EDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVSIN 62
           E+L  DLEE++ L   + R R+   +++E S +E   +  + Q     P   +    ++ 
Sbjct: 6   EELQKDLEEVKVLLEKSTRKRLRDTLTNEKSKIETELRNKMQQKSQKKPEFDNEKPAAVV 65

Query: 63  SGIV--YT-PLPGFSWDQDNDKVKIYVSLEGIED---EKVEADYKKLSIDIKFHDVKGKN 122
           + +   YT  +  + WDQ +  VKIY++L G+     E V+  + + S D+   ++ GKN
Sbjct: 66  APLTTGYTVKISNYGWDQSDKFVKIYITLTGVHQVPAENVQVHFTERSFDLLVKNLNGKN 125

Query: 123 YRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLK-----EEKLKPGLDKE 182
           Y   +  L K I  E     VK    +I   K ++    D   +     +EK KP  D E
Sbjct: 126 YSMIVNNLLKPISVESSSKKVKTDTVIILCRKKAENTRWDYLTQVEKECKEKEKPSYDTE 185

Query: 183 RDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDAR 206
            DP  G+M+++K +Y++GDD+MKRTI KAW ++R
Sbjct: 186 ADPSEGLMNVLKKIYEDGDDDMKRTINKAWVESR 219

BLAST of Carg19939 vs. ExPASy Swiss-Prot
Match: Q4R4P3 (Calcyclin-binding protein OS=Macaca fascicularis OX=9541 GN=CACYBP PE=2 SV=1)

HSP 1 Score: 99.8 bits (247), Expect = 4.1e-20
Identity = 70/217 (32.26%), Postives = 110/217 (50.69%), Query Frame = 0

Query: 2   AEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAK--- 61
           +E+L  DLEE++ L   A R RV   +++E S +E   K  + Q        +   K   
Sbjct: 3   SEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAA 62

Query: 62  --VSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIED---EKVEADYKKLSIDIKFHDVK 121
               I +G     +  + WDQ +  VKIY++L G+     E V+  + + S D+   ++ 
Sbjct: 63  VVAPITTGYT-VKISNYGWDQSDKFVKIYITLSGVHQVPTENVQVHFTERSFDLLVKNLN 122

Query: 122 GKNYRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKA---SKGNWLDLHLKE--EKLKPGL 181
           GK+Y   +  L K I  E     VK    +I   K    ++ ++L    KE  EK KP  
Sbjct: 123 GKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSY 182

Query: 182 DKERDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDAR 206
           D E DP  G+M+++K +Y++GDD+MKRTI KAW ++R
Sbjct: 183 DTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESR 218

BLAST of Carg19939 vs. ExPASy Swiss-Prot
Match: Q9HB71 (Calcyclin-binding protein OS=Homo sapiens OX=9606 GN=CACYBP PE=1 SV=2)

HSP 1 Score: 99.4 bits (246), Expect = 5.4e-20
Identity = 70/217 (32.26%), Postives = 110/217 (50.69%), Query Frame = 0

Query: 2   AEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAK--- 61
           +E+L  DLEE++ L   A R RV   +++E S +E   K  + Q        +   K   
Sbjct: 3   SEELQKDLEEVKVLLEKATRKRVRDALTAEKSKIETEIKNKMQQKSQKKAELLDNEKPAA 62

Query: 62  --VSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIED---EKVEADYKKLSIDIKFHDVK 121
               I +G     +  + WDQ +  VKIY++L G+     E V+  + + S D+   ++ 
Sbjct: 63  VVAPITTGYT-VKISNYGWDQSDKFVKIYITLTGVHQVPTENVQVHFTERSFDLLVKNLN 122

Query: 122 GKNYRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKA---SKGNWLDLHLKE--EKLKPGL 181
           GK+Y   +  L K I  E     VK    +I   K    ++ ++L    KE  EK KP  
Sbjct: 123 GKSYSMIVNNLLKPISVEGSSKKVKTDTVLILCRKKVENTRWDYLTQVEKECKEKEKPSY 182

Query: 182 DKERDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDAR 206
           D E DP  G+M+++K +Y++GDD+MKRTI KAW ++R
Sbjct: 183 DTETDPSEGLMNVLKKIYEDGDDDMKRTINKAWVESR 218

BLAST of Carg19939 vs. ExPASy TrEMBL
Match: A0A6J1EPD4 (Calcyclin-binding protein OS=Cucurbita moschata OX=3662 GN=LOC111434368 PE=4 SV=1)

HSP 1 Score: 423.7 bits (1088), Expect = 4.6e-115
Identity = 213/217 (98.16%), Postives = 216/217 (99.54%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRV+SLISSEISNLEKLSKETVPQTHASTPASISTAKVS
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVLSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDND+VKIYVSLEGIE EKVEADYKKLSIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDRVKIYVSLEGIEQEKVEADYKKLSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKE+KLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEDKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. ExPASy TrEMBL
Match: A0A6J1KQL9 (Calcyclin-binding protein OS=Cucurbita maxima OX=3661 GN=LOC111495594 PE=4 SV=1)

HSP 1 Score: 417.2 bits (1071), Expect = 4.3e-113
Identity = 210/217 (96.77%), Postives = 212/217 (97.70%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELRHLHSIAKRPRV+SLISSEISNLEKLSKETVPQTHA TP SISTAKV 
Sbjct: 1   MAEDLSLDLEELRHLHSIAKRPRVLSLISSEISNLEKLSKETVPQTHAPTPVSISTAKVP 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIE EKVEADYKK SIDIKFHDVKGKNYRFA
Sbjct: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEQEKVEADYKKQSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKE+KLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEDKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP
Sbjct: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 217

BLAST of Carg19939 vs. ExPASy TrEMBL
Match: A0A5D3DAQ7 (Calcyclin-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold480G00540 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 8.2e-104
Identity = 195/219 (89.04%), Postives = 206/219 (94.06%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASIST--AK 60
           MAEDLSLDLEELRHLH+IAKRPR+VSLISS+I  LE+LSKE+VPQT   TP  +ST  AK
Sbjct: 1   MAEDLSLDLEELRHLHTIAKRPRIVSLISSQIRTLEQLSKESVPQTPTPTPIPVSTSVAK 60

Query: 61  VSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYR 120
           V INS IVYTPLPGFSWDQDNDKVKIY+SLEGIE EKVEADYK+ SIDIKFHDVKGKNYR
Sbjct: 61  VPINSPIVYTPLPGFSWDQDNDKVKIYISLEGIEQEKVEADYKQSSIDIKFHDVKGKNYR 120

Query: 121 FAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGI 180
           FAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDL+LKE+KLKPGLDKERDPMAGI
Sbjct: 121 FAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLNLKEDKLKPGLDKERDPMAGI 180

Query: 181 MDLMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           MDLMKNMYDEGDDEMKRTIAKAWTDAR+GKTADPLKGFP
Sbjct: 181 MDLMKNMYDEGDDEMKRTIAKAWTDARTGKTADPLKGFP 219

BLAST of Carg19939 vs. ExPASy TrEMBL
Match: A0A1S3BA68 (Calcyclin-binding protein OS=Cucumis melo OX=3656 GN=LOC103487695 PE=4 SV=1)

HSP 1 Score: 386.3 bits (991), Expect = 8.2e-104
Identity = 195/219 (89.04%), Postives = 206/219 (94.06%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASIST--AK 60
           MAEDLSLDLEELRHLH+IAKRPR+VSLISS+I  LE+LSKE+VPQT   TP  +ST  AK
Sbjct: 1   MAEDLSLDLEELRHLHTIAKRPRIVSLISSQIRTLEQLSKESVPQTPTPTPIPVSTSVAK 60

Query: 61  VSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYR 120
           V INS IVYTPLPGFSWDQDNDKVKIY+SLEGIE EKVEADYK+ SIDIKFHDVKGKNYR
Sbjct: 61  VPINSPIVYTPLPGFSWDQDNDKVKIYISLEGIEQEKVEADYKQSSIDIKFHDVKGKNYR 120

Query: 121 FAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGI 180
           FAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDL+LKE+KLKPGLDKERDPMAGI
Sbjct: 121 FAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLNLKEDKLKPGLDKERDPMAGI 180

Query: 181 MDLMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           MDLMKNMYDEGDDEMKRTIAKAWTDAR+GKTADPLKGFP
Sbjct: 181 MDLMKNMYDEGDDEMKRTIAKAWTDARTGKTADPLKGFP 219

BLAST of Carg19939 vs. ExPASy TrEMBL
Match: A0A0A0LUF8 (Calcyclin-binding protein OS=Cucumis sativus OX=3659 GN=Csa_1G439330 PE=4 SV=1)

HSP 1 Score: 377.5 bits (968), Expect = 3.8e-101
Identity = 188/217 (86.64%), Postives = 203/217 (93.55%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKLSKETVPQTHASTPASISTAKVS 60
           MAEDLSLDLEELR LH+IAKRPRV+SLI+S+I  LE+LS+++VP+T    P S S AKV 
Sbjct: 1   MAEDLSLDLEELRLLHTIAKRPRVISLITSQIRTLEQLSEQSVPRTPTPIPVSTSIAKVP 60

Query: 61  INSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKGKNYRFA 120
           INS IVYTPLPGFSWDQDNDKVKIY+SLEGIE EKVEADYK+LSIDIKFHDVKGKNYRFA
Sbjct: 61  INSSIVYTPLPGFSWDQDNDKVKIYISLEGIEQEKVEADYKQLSIDIKFHDVKGKNYRFA 120

Query: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDPMAGIMD 180
           IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNW DL+LKE+KLKPGLDKERDPMAGIMD
Sbjct: 121 IPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWSDLNLKEDKLKPGLDKERDPMAGIMD 180

Query: 181 LMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKGFP 218
           LMKNMYD+GDDEMKRTIAKAWTDAR+GKTADPLKGFP
Sbjct: 181 LMKNMYDDGDDEMKRTIAKAWTDARTGKTADPLKGFP 217

BLAST of Carg19939 vs. TAIR 10
Match: AT1G30070.1 (SGS domain-containing protein )

HSP 1 Score: 288.1 bits (736), Expect = 5.8e-78
Identity = 142/221 (64.25%), Postives = 183/221 (82.81%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKL-----SKETVPQTHASTPASIS 60
           MAE++ LDLEELR L +IAKRPRV++LI+SEISNLEKL     S    P+   + PA +S
Sbjct: 1   MAEEVGLDLEELRQLQNIAKRPRVLNLINSEISNLEKLRDSAVSSNAKPKVPVTVPAPVS 60

Query: 61  TA-KVSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKG 120
           ++ K   +S + Y  L  FSWDQDNDKVK+Y+SLEG++++KV+A++K +S+DIK HDV+G
Sbjct: 61  SSGKPVSSSALNYVTLGTFSWDQDNDKVKMYISLEGVDEDKVQAEFKPMSLDIKIHDVQG 120

Query: 121 KNYRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEKLKPGLDKERDP 180
           KNYR AIPKL KEI+PEKCK+LVKP R VIT+ K+S+GNWLD+H KE+K+KP L+KE+DP
Sbjct: 121 KNYRCAIPKLCKEIMPEKCKVLVKPKRIVITMVKSSRGNWLDIHHKEDKIKPSLEKEKDP 180

Query: 181 MAGIMDLMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKG 216
           MAGIM +MKN+Y++GD+EMK+TIAKAWTDARSGK ADPLKG
Sbjct: 181 MAGIMGMMKNLYEDGDEEMKKTIAKAWTDARSGKAADPLKG 221

BLAST of Carg19939 vs. TAIR 10
Match: AT1G30070.2 (SGS domain-containing protein )

HSP 1 Score: 281.2 bits (718), Expect = 7.1e-76
Identity = 142/228 (62.28%), Postives = 183/228 (80.26%), Query Frame = 0

Query: 1   MAEDLSLDLEELRHLHSIAKRPRVVSLISSEISNLEKL-----SKETVPQTHASTPASIS 60
           MAE++ LDLEELR L +IAKRPRV++LI+SEISNLEKL     S    P+   + PA +S
Sbjct: 1   MAEEVGLDLEELRQLQNIAKRPRVLNLINSEISNLEKLRDSAVSSNAKPKVPVTVPAPVS 60

Query: 61  TA-KVSINSGIVYTPLPGFSWDQDNDKVKIYVSLEGIEDEKVEADYKKLSIDIKFHDVKG 120
           ++ K   +S + Y  L  FSWDQDNDKVK+Y+SLEG++++KV+A++K +S+DIK HDV+G
Sbjct: 61  SSGKPVSSSALNYVTLGTFSWDQDNDKVKMYISLEGVDEDKVQAEFKPMSLDIKIHDVQG 120

Query: 121 KNYRFAIPKLNKEIVPEKCKLLVKPTRAVITLYKASKGNWLDLHLKEEK-------LKPG 180
           KNYR AIPKL KEI+PEKCK+LVKP R VIT+ K+S+GNWLD+H KE+K       +KP 
Sbjct: 121 KNYRCAIPKLCKEIMPEKCKVLVKPKRIVITMVKSSRGNWLDIHHKEDKYCLLFVQIKPS 180

Query: 181 LDKERDPMAGIMDLMKNMYDEGDDEMKRTIAKAWTDARSGKTADPLKG 216
           L+KE+DPMAGIM +MKN+Y++GD+EMK+TIAKAWTDARSGK ADPLKG
Sbjct: 181 LEKEKDPMAGIMGMMKNLYEDGDEEMKKTIAKAWTDARSGKAADPLKG 228

BLAST of Carg19939 vs. TAIR 10
Match: AT1G30060.1 (COP1-interacting protein-related )

HSP 1 Score: 55.1 bits (131), Expect = 8.3e-08
Identity = 24/28 (85.71%), Postives = 27/28 (96.43%), Query Frame = 0

Query: 188 EGDDEMKRTIAKAWTDARSGKTADPLKG 216
           +GD+EMK+TIAKAWTDARSGK ADPLKG
Sbjct: 148 DGDEEMKKTIAKAWTDARSGKAADPLKG 175

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7019517.11.7e-116100.00Calcyclin-binding protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
KAG6583900.11.9e-11599.08Calcyclin-binding protein, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022927580.19.6e-11598.16calcyclin-binding protein [Cucurbita moschata][more]
XP_023001468.18.9e-11396.77calcyclin-binding protein [Cucurbita maxima][more]
XP_023520274.11.5e-11297.24calcyclin-binding protein [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
Q9CXW32.2e-2131.78Calcyclin-binding protein OS=Mus musculus OX=10090 GN=Cacybp PE=1 SV=1[more]
Q3T1681.1e-2033.33Calcyclin-binding protein OS=Bos taurus OX=9913 GN=CACYBP PE=2 SV=1[more]
Q6AYK61.1e-2030.84Calcyclin-binding protein OS=Rattus norvegicus OX=10116 GN=Cacybp PE=1 SV=1[more]
Q4R4P34.1e-2032.26Calcyclin-binding protein OS=Macaca fascicularis OX=9541 GN=CACYBP PE=2 SV=1[more]
Q9HB715.4e-2032.26Calcyclin-binding protein OS=Homo sapiens OX=9606 GN=CACYBP PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1EPD44.6e-11598.16Calcyclin-binding protein OS=Cucurbita moschata OX=3662 GN=LOC111434368 PE=4 SV=... [more]
A0A6J1KQL94.3e-11396.77Calcyclin-binding protein OS=Cucurbita maxima OX=3661 GN=LOC111495594 PE=4 SV=1[more]
A0A5D3DAQ78.2e-10489.04Calcyclin-binding protein OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo... [more]
A0A1S3BA688.2e-10489.04Calcyclin-binding protein OS=Cucumis melo OX=3656 GN=LOC103487695 PE=4 SV=1[more]
A0A0A0LUF83.8e-10186.64Calcyclin-binding protein OS=Cucumis sativus OX=3659 GN=Csa_1G439330 PE=4 SV=1[more]
Match NameE-valueIdentityDescription
AT1G30070.15.8e-7864.25SGS domain-containing protein [more]
AT1G30070.27.1e-7662.28SGS domain-containing protein [more]
AT1G30060.18.3e-0885.71COP1-interacting protein-related [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR007052CS domainPFAMPF04969CScoord: 73..148
e-value: 7.8E-13
score: 49.3
IPR007052CS domainPROSITEPS51203CScoord: 68..158
score: 13.463943
IPR008978HSP20-like chaperoneGENE3D2.60.40.790coord: 67..166
e-value: 2.9E-25
score: 90.5
IPR008978HSP20-like chaperoneSUPERFAMILY49764HSP20-like chaperonescoord: 70..202
IPR015120Siah interacting protein, N-terminalPFAMPF09032Siah-Interact_Ncoord: 3..50
e-value: 9.5E-7
score: 29.0
NoneNo IPR availablePANTHERPTHR47686SGS DOMAIN-CONTAINING PROTEINcoord: 1..216
IPR007699SGS domainPROSITEPS51048SGScoord: 143..217
score: 16.496246
IPR037893Calcyclin-binding Protein, CS domainCDDcd06468p23_CacyBPcoord: 73..158
e-value: 3.56336E-31
score: 107.734
IPR037201Calcyclin-binding protein, N-terminalSUPERFAMILY140106Calcyclin-binding protein-likecoord: 3..37

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19939-RACarg19939-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
molecular_function GO:0044548 S100 protein binding
molecular_function GO:0015631 tubulin binding
molecular_function GO:0031625 ubiquitin protein ligase binding