Carg19853 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19853
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionTranscription elongation factor spt6
LocationCarg_Chr05: 5051808 .. 5053423 (-)
RNA-Seq ExpressionCarg19853
SyntenyCarg19853
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGACGCTGGTAATGATCAGGATAAAAATGATAAAACACCAACTTTAAAGTGGCATAAGGTGAGTAAGAGTGTAATTGGAAGTAGTGTTTTGCTTGTATGGATTGGAAAATTAAGATGCAATGTGCATTTACTTTTCATTTTAGGTACTTTGGGCAATTCAAGACTTGGACAAAAAGTGGCTGCTTCTTCAAAAGAGAAAAAAGGCTCTTCACTTATATTATAGAAAGCGATTTGAGGAAGAGTTACGCAGGGATGATGATGTAACTAGACAATCATTGAATAGCCAGCTTTTTGAGTCAGTCAAGAAATCTCTTGAGGCAGCTGAATCAGAGAGAGAGGTAGATGATGTTGACTCAAAGTTTAATTTGCATTTTCCTCCAGGTGAAGTTGGTGTTGATGAAGGACAGTTCAAAAGGCCTAAGAGGAAATCACTGTATAGTATATGTAGTAAGGCTGGTTTATGGGAGGTTGCAGGAAAATTTGGTTATAGCTCTGAGCAATTCGGGTTGCAACTCTCCTTAGAAAAGATGGTTAGTGGTCTTTGATATGATTTTGTTTCTAATCTTGGATATGCTAATGACATCTGTTCAATTCTTTGTAGAGAAATGATGAGTTGGAGGATCCAAAGGAAACTCCTGAAGAGATGGCATCAAACTTTACCTGTGCGATGTTTGAGTCTCCTCAAGCTGTACTCAAGGGAGCAAGACATATGGTATTATAATTTTGTTTCCTTAGATTTAATTATTGAATGCTGCATAGTGTTATTCATGGTCTTTTCTGTCTCTTCATGTGATTTCAGGCAGCTATTGAGATTAGTTGTGAACCTTGTGTAAGGAAGCATGTCAGGAGCTACTTTATGGACTATGCTGTGATATCAACAAATCCTACGTCAGATGGAAATGCTGCAATAGATTCGTTTCATCAATTTTCAGTTGTTAAGTGGCTCCGTGAAAAGCCATTAAGTAGATTTGAGGATGCACAGTGGCTTCTTATTCAGAAGGCTGAAGAAGAAAAACTTCTGAATGTCACTCTCAAGCTACCTGAAAAGCATTTAAACAAGTTGATAAATGATTTTAATGAATATTATCTCAGTGATGGTGTTAGCAAATCTGCTCAGCTTTGGAATGAGCAGAGAAAGTTAATATTGCAGGATGCACTTTCTGGTTTTCTTCTGCCCTCAATGGAGAAAGAAGCAAGATCCGTGATGACTAGTAAAGCAAAGAAGTGGTTACTTACGGAATATGGGAAGAATTTATGGAATAAAATATCTGTTGGTCCATATCAGCACAAAGAAAATGATATTAGTTCTGATGAAGAAGCTGCCCCAAGGGTAATGGCATGCTGTTGGGGTCCTGGAAAGCCAGCTACCACCTTTGTGATGTTAGACTCAGCAGGTGAGGTGCTTGATGTTCTTTATACTGGATCTCTCACCCTACGTTCTCAGAATGTGAATGACCAACAAAGGAAGAAAAATGATCAAGAGCGAGTTTTGAAGTTCATGACAGATCATCAACCACATGTTGTTGTTTTAGGAGCTGTTAATTTATCTTGTACCCGATTGAAAGATGATATCTACGAGGTTAGTGCTCCTATAACTAGTGGTGCTGATCATTAA

mRNA sequence

ATGACGCTGGTACTTTGGGCAATTCAAGACTTGGACAAAAAGTGGCTGCTTCTTCAAAAGAGAAAAAAGGCTCTTCACTTATATTATAGAAAGCGATTTGAGGAAGAGTTACGCAGGGATGATGATGTAACTAGACAATCATTGAATAGCCAGCTTTTTGAGTCAGTCAAGAAATCTCTTGAGGCAGCTGAATCAGAGAGAGAGGTAGATGATGTTGACTCAAAGTTTAATTTGCATTTTCCTCCAGGTGAAGTTGGTGTTGATGAAGGACAGTTCAAAAGGCCTAAGAGGAAATCACTGTATAGTATATGTAGTAAGGCTGGTTTATGGGAGGTTGCAGGAAAATTTGGTTATAGCTCTGAGCAATTCGGGTTGCAACTCTCCTTAGAAAAGATGAGAAATGATGAGTTGGAGGATCCAAAGGAAACTCCTGAAGAGATGGCATCAAACTTTACCTGTGCGATGTTTGAGTCTCCTCAAGCTGTACTCAAGGGAGCAAGACATATGGCAGCTATTGAGATTAGTTGTGAACCTTGTGTAAGGAAGCATGTCAGGAGCTACTTTATGGACTATGCTGTGATATCAACAAATCCTACGTCAGATGGAAATGCTGCAATAGATTCGTTTCATCAATTTTCAGTTGTTAAGTGGCTCCGTGAAAAGCCATTAAGTAGATTTGAGGATGCACAGTGGCTTCTTATTCAGAAGGCTGAAGAAGAAAAACTTCTGAATGTCACTCTCAAGCTACCTGAAAAGCATTTAAACAAGTTGATAAATGATTTTAATGAATATTATCTCAGTGATGGTGTTAGCAAATCTGCTCAGCTTTGGAATGAGCAGAGAAAGTTAATATTGCAGGATGCACTTTCTGGTTTTCTTCTGCCCTCAATGGAGAAAGAAGCAAGATCCGTGATGACTAGTAAAGCAAAGAAGTGGTTACTTACGGAATATGGGAAGAATTTATGGAATAAAATATCTGTTGGTCCATATCAGCACAAAGAAAATGATATTAGTTCTGATGAAGAAGCTGCCCCAAGGGTAATGGCATGCTGTTGGGGTCCTGGAAAGCCAGCTACCACCTTTGTGATGTTAGACTCAGCAGGTGAGGTGCTTGATGTTCTTTATACTGGATCTCTCACCCTACGTTCTCAGAATGTGAATGACCAACAAAGGAAGAAAAATGATCAAGAGCGAGTTTTGAAGTTCATGACAGATCATCAACCACATGTTGTTGTTTTAGGAGCTGTTAATTTATCTTGTACCCGATTGAAAGATGATATCTACGAGGTTAGTGCTCCTATAACTAGTGGTGCTGATCATTAA

Coding sequence (CDS)

ATGACGCTGGTACTTTGGGCAATTCAAGACTTGGACAAAAAGTGGCTGCTTCTTCAAAAGAGAAAAAAGGCTCTTCACTTATATTATAGAAAGCGATTTGAGGAAGAGTTACGCAGGGATGATGATGTAACTAGACAATCATTGAATAGCCAGCTTTTTGAGTCAGTCAAGAAATCTCTTGAGGCAGCTGAATCAGAGAGAGAGGTAGATGATGTTGACTCAAAGTTTAATTTGCATTTTCCTCCAGGTGAAGTTGGTGTTGATGAAGGACAGTTCAAAAGGCCTAAGAGGAAATCACTGTATAGTATATGTAGTAAGGCTGGTTTATGGGAGGTTGCAGGAAAATTTGGTTATAGCTCTGAGCAATTCGGGTTGCAACTCTCCTTAGAAAAGATGAGAAATGATGAGTTGGAGGATCCAAAGGAAACTCCTGAAGAGATGGCATCAAACTTTACCTGTGCGATGTTTGAGTCTCCTCAAGCTGTACTCAAGGGAGCAAGACATATGGCAGCTATTGAGATTAGTTGTGAACCTTGTGTAAGGAAGCATGTCAGGAGCTACTTTATGGACTATGCTGTGATATCAACAAATCCTACGTCAGATGGAAATGCTGCAATAGATTCGTTTCATCAATTTTCAGTTGTTAAGTGGCTCCGTGAAAAGCCATTAAGTAGATTTGAGGATGCACAGTGGCTTCTTATTCAGAAGGCTGAAGAAGAAAAACTTCTGAATGTCACTCTCAAGCTACCTGAAAAGCATTTAAACAAGTTGATAAATGATTTTAATGAATATTATCTCAGTGATGGTGTTAGCAAATCTGCTCAGCTTTGGAATGAGCAGAGAAAGTTAATATTGCAGGATGCACTTTCTGGTTTTCTTCTGCCCTCAATGGAGAAAGAAGCAAGATCCGTGATGACTAGTAAAGCAAAGAAGTGGTTACTTACGGAATATGGGAAGAATTTATGGAATAAAATATCTGTTGGTCCATATCAGCACAAAGAAAATGATATTAGTTCTGATGAAGAAGCTGCCCCAAGGGTAATGGCATGCTGTTGGGGTCCTGGAAAGCCAGCTACCACCTTTGTGATGTTAGACTCAGCAGGTGAGGTGCTTGATGTTCTTTATACTGGATCTCTCACCCTACGTTCTCAGAATGTGAATGACCAACAAAGGAAGAAAAATGATCAAGAGCGAGTTTTGAAGTTCATGACAGATCATCAACCACATGTTGTTGTTTTAGGAGCTGTTAATTTATCTTGTACCCGATTGAAAGATGATATCTACGAGGTTAGTGCTCCTATAACTAGTGGTGCTGATCATTAA

Protein sequence

MTLVLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAAESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQFGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKHVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLLNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARSVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVMLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRLKDDIYEVSAPITSGADH
Homology
BLAST of Carg19853 vs. NCBI nr
Match: KAG7029903.1 (Transcription elongation factor SPT6-like protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 881.3 bits (2276), Expect = 3.4e-252
Identity = 440/440 (100.00%), Postives = 440/440 (100.00%), Query Frame = 0

Query: 1   MTLVLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSL 60
           MTLVLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSL
Sbjct: 1   MTLVLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSL 60

Query: 61  EAAESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSS 120
           EAAESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSS
Sbjct: 61  EAAESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSS 120

Query: 121 EQFGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCV 180
           EQFGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCV
Sbjct: 121 EQFGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCV 180

Query: 181 RKHVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEE 240
           RKHVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEE
Sbjct: 181 RKHVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEE 240

Query: 241 KLLNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKE 300
           KLLNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKE
Sbjct: 241 KLLNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKE 300

Query: 301 ARSVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATT 360
           ARSVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATT
Sbjct: 301 ARSVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATT 360

Query: 361 FVMLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSC 420
           FVMLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSC
Sbjct: 361 FVMLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSC 420

Query: 421 TRLKDDIYEVSAPITSGADH 441
           TRLKDDIYEVSAPITSGADH
Sbjct: 421 TRLKDDIYEVSAPITSGADH 440

BLAST of Carg19853 vs. NCBI nr
Match: XP_022929650.1 (transcription elongation factor SPT6 homolog [Cucurbita moschata])

HSP 1 Score: 855.5 bits (2209), Expect = 2.0e-244
Identity = 426/427 (99.77%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA
Sbjct: 390 VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 449

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF
Sbjct: 450 ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 509

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH
Sbjct: 510 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 569

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL
Sbjct: 570 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 629

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
           NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS
Sbjct: 630 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 689

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM
Sbjct: 690 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 749

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL
Sbjct: 750 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 809

Query: 424 KDDIYEV 431
           KDDIYE+
Sbjct: 810 KDDIYEI 816

BLAST of Carg19853 vs. NCBI nr
Match: XP_023545891.1 (transcription elongation factor SPT6 homolog [Cucurbita pepo subsp. pepo])

HSP 1 Score: 852.0 bits (2200), Expect = 2.2e-243
Identity = 423/427 (99.06%), Postives = 426/427 (99.77%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           +LWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLF+SVKKSLEAA
Sbjct: 386 ILWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFDSVKKSLEAA 445

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF
Sbjct: 446 ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 505

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH
Sbjct: 506 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 565

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL
Sbjct: 566 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 625

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
           NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS
Sbjct: 626 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 685

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           VMTSKAKKWLLTEYGKNLWNKIS GPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM
Sbjct: 686 VMTSKAKKWLLTEYGKNLWNKISFGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 745

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL
Sbjct: 746 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 805

Query: 424 KDDIYEV 431
           KDDIYE+
Sbjct: 806 KDDIYEI 812

BLAST of Carg19853 vs. NCBI nr
Match: XP_022996607.1 (transcription elongation factor SPT6 homolog isoform X1 [Cucurbita maxima])

HSP 1 Score: 846.7 bits (2186), Expect = 9.3e-242
Identity = 424/428 (99.07%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLF+SVKKSLEAA
Sbjct: 387 VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFDSVKKSLEAA 446

Query: 64  ESEREVDDVDSKFNLHFPPGEVG-VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 123
           ESEREVDDVDSKFNLHFP GEVG VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ
Sbjct: 447 ESEREVDDVDSKFNLHFPTGEVGVVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 506

Query: 124 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 183
           FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK
Sbjct: 507 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 566

Query: 184 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 243
           HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL
Sbjct: 567 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 626

Query: 244 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 303
           LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR
Sbjct: 627 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 686

Query: 304 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 363
           SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV
Sbjct: 687 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 746

Query: 364 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 423
           MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR
Sbjct: 747 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 806

Query: 424 LKDDIYEV 431
           LKDDIYE+
Sbjct: 807 LKDDIYEI 814

BLAST of Carg19853 vs. NCBI nr
Match: XP_022996608.1 (transcription elongation factor SPT6 homolog isoform X2 [Cucurbita maxima])

HSP 1 Score: 846.7 bits (2186), Expect = 9.3e-242
Identity = 424/428 (99.07%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLF+SVKKSLEAA
Sbjct: 369 VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFDSVKKSLEAA 428

Query: 64  ESEREVDDVDSKFNLHFPPGEVG-VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 123
           ESEREVDDVDSKFNLHFP GEVG VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ
Sbjct: 429 ESEREVDDVDSKFNLHFPTGEVGVVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 488

Query: 124 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 183
           FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK
Sbjct: 489 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 548

Query: 184 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 243
           HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL
Sbjct: 549 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 608

Query: 244 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 303
           LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR
Sbjct: 609 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 668

Query: 304 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 363
           SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV
Sbjct: 669 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 728

Query: 364 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 423
           MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR
Sbjct: 729 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 788

Query: 424 LKDDIYEV 431
           LKDDIYE+
Sbjct: 789 LKDDIYEI 796

BLAST of Carg19853 vs. ExPASy Swiss-Prot
Match: A8MS85 (Transcription elongation factor SPT6 homolog OS=Arabidopsis thaliana OX=3702 GN=SPT6 PE=1 SV=1)

HSP 1 Score: 670.2 bits (1728), Expect = 1.6e-191
Identity = 335/427 (78.45%), Postives = 371/427 (86.89%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           V W I DLDKKWLLL+KRK ALH YY KR+EEE RR  D TR +LN  LFESV KSL+ A
Sbjct: 376 VFWMIHDLDKKWLLLRKRKMALHGYYTKRYEEESRRVYDETRLNLNQYLFESVIKSLKVA 435

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           E+EREVDDVDSKFNLHFPPGE+GVDEGQ+KRPKRKS YSICSKAGLWEVA KFGYS+EQ 
Sbjct: 436 ETEREVDDVDSKFNLHFPPGEIGVDEGQYKRPKRKSQYSICSKAGLWEVANKFGYSAEQL 495

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GL LSLEK+  DELED KETPEEMA NF CAMFE+  AVLKGARHMAA+EISCEP V+K+
Sbjct: 496 GLALSLEKL-VDELEDAKETPEEMAKNFVCAMFENSLAVLKGARHMAAVEISCEPSVKKY 555

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VR  +M+ AV+ST+PT+DGN  IDSFHQFS +KWLREKPLS+FE AQWLLIQK EEEKLL
Sbjct: 556 VRGIYMENAVVSTSPTADGNTVIDSFHQFSGIKWLREKPLSKFEGAQWLLIQKGEEEKLL 615

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
            VT KLPE ++N+LI+D NE+YLS GVSK AQLWNEQRKLIL+DAL  FLLPSMEKEARS
Sbjct: 616 QVTFKLPENYMNRLISDCNEHYLSVGVSKYAQLWNEQRKLILEDALHAFLLPSMEKEARS 675

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           ++TS+AK  LL+EYG+ LWNK+S GPYQ KE DI+ DEEAAPRVMACCWGPGKP  TFVM
Sbjct: 676 LLTSRAKSRLLSEYGQALWNKVSAGPYQKKEMDINLDEEAAPRVMACCWGPGKPPNTFVM 735

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GEVLDVLY GSLT RSQNVNDQQRKK+DQ+RVLKFM DHQPHVV LGAVNLSCTRL
Sbjct: 736 LDSSGEVLDVLYAGSLTSRSQNVNDQQRKKSDQDRVLKFMMDHQPHVVALGAVNLSCTRL 795

Query: 424 KDDIYEV 431
           KDDIYEV
Sbjct: 796 KDDIYEV 801

BLAST of Carg19853 vs. ExPASy Swiss-Prot
Match: Q9CAM1 (Transcription elongation factor SPT6-like OS=Arabidopsis thaliana OX=3702 GN=At1g63210 PE=1 SV=2)

HSP 1 Score: 583.9 bits (1504), Expect = 1.5e-165
Identity = 296/426 (69.48%), Postives = 349/426 (81.92%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLW IQDLD+KW+LL+KRK AL  YY KRFEEE    D      LN  LFESV KSL+AA
Sbjct: 300 VLWMIQDLDRKWVLLRKRKTALFGYYTKRFEEETCMSD------LNKSLFESVIKSLQAA 359

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           E+EREVDDVDSKFNLHFP  E  +DEGQ+KRP RKS YSICSK G+ E A KFGYS+EQ 
Sbjct: 360 ETEREVDDVDSKFNLHFPHCE--IDEGQYKRPNRKSQYSICSKFGIKEFANKFGYSAEQL 419

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GL LSLEK+  DELED K+TPEEMA N+ CAMF   QAVLKGARHMAA+EISCEP +RK+
Sbjct: 420 GLALSLEKVFVDELEDAKKTPEEMALNYECAMFGDSQAVLKGARHMAAVEISCEPSIRKY 479

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VR  FM+ AV+ST+PT DGN  IDSFH+FS VKWL EKPL +F+  QWLLIQKAEEEKLL
Sbjct: 480 VRGIFMENAVVSTSPTPDGNVVIDSFHRFSAVKWLSEKPLRKFDGVQWLLIQKAEEEKLL 539

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
            VT KLPE  +N++I++ +E+YLS GVSK AQLWNEQRKLIL+DA+ GF+LPSMEKEARS
Sbjct: 540 QVTFKLPENQMNRIISECSEHYLSVGVSKYAQLWNEQRKLILEDAVHGFVLPSMEKEARS 599

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           ++TS+AK  LL+EYG+ LWNK+S GPY+   N  +S+EEAAPRV+ACCWGPG P TTFVM
Sbjct: 600 LLTSRAKTQLLSEYGQVLWNKVSSGPYRRNNN--TSEEEAAPRVLACCWGPGNPETTFVM 659

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GE++DVLY+GS+ LRS++VNDQ+RKK DQ+R LKF+ DHQP V+ L AVNLSC RL
Sbjct: 660 LDSSGELVDVLYSGSIGLRSRDVNDQKRKKMDQDRFLKFIRDHQPDVLALAAVNLSCVRL 715

Query: 424 KDDIYE 430
           KD+IYE
Sbjct: 720 KDEIYE 715

BLAST of Carg19853 vs. ExPASy Swiss-Prot
Match: Q62383 (Transcription elongation factor SPT6 OS=Mus musculus OX=10090 GN=Supt6h PE=1 SV=2)

HSP 1 Score: 186.4 bits (472), Expect = 6.8e-46
Identity = 139/483 (28.78%), Postives = 227/483 (47.00%), Query Frame = 0

Query: 5   LWAIQDLDKKWLLLQKRKKALHLYYRKRFE---EELRRDDDVTRQSLNSQLFESVKKSLE 64
           LW +   D+KW  L+ RK+ L   + K      E++  D D         L  +  + L+
Sbjct: 393 LWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLK 452

Query: 65  AAESEREVDDVDSKFNLHF----------------------------PPGEVGVDEGQ-- 124
             +S  E+ DV + F L++                              GE   DE Q  
Sbjct: 453 DVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRIKEDGDEEGEGEEAEDEEQRG 512

Query: 125 --FKRPKRKSLYSICSKAGLWEVAGKFGYSSEQFGLQLSLEKMRNDELEDPKETPEEMAS 184
              K+  R+ +Y+IC  AGL  +A KFG + EQFG  L     R++  + P E P E+A 
Sbjct: 513 PELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAE-PLELAK 572

Query: 185 NFTCAMFESPQAVLKGARHMAAIEISCEPCVRKHVRSYFMDYAVISTNPTSDGNAAIDSF 244
           ++ C+ F +P+AVL+GAR+M A++I+ EP VR+ +R  F + A ++  PT  G   +D  
Sbjct: 573 DYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEA 632

Query: 245 HQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLLNVTLKLPEKHLNKLIND------FNE 304
           H     K+L+ KP+    D Q+L I  AE+E LL + + +  K +    ND        +
Sbjct: 633 HYAYSFKYLKNKPVKELRDDQFLKIGLAEDEGLLTIDISIDMKGVEGYGNDQTYFEEIKQ 692

Query: 305 YYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARSVMTSKAKKWLLTEYGKNLWN 364
           +Y  D  S   Q WN QR + ++ AL  FL   M KE ++ + ++A++ ++    + L+N
Sbjct: 693 FYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEARESVVKACSRKLYN 752

Query: 365 KISVGPY----QHKENDISSDEEAAP--RVMACCWGPGKPATTF-VMLDSAGEVLDVLYT 424
            + V PY    Q +E+D   DE      RV+   +   +    F  +++  GEV D L  
Sbjct: 753 WLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRL 812

Query: 425 GSLTLRSQNVNDQQRKK--NDQERVLKFMTDHQPHVVVLGAVNLSCTRLKDDIYEVSAPI 438
              T R     +++R+K   D E + KF+ + +PHVV +   N     L +D+  +   +
Sbjct: 813 PHFTKRRTAWREEEREKKAQDIETLKKFLVNKKPHVVTIAGENRDAQMLTEDVKRIVHEL 872

BLAST of Carg19853 vs. ExPASy Swiss-Prot
Match: Q7KZ85 (Transcription elongation factor SPT6 OS=Homo sapiens OX=9606 GN=SUPT6H PE=1 SV=2)

HSP 1 Score: 185.7 bits (470), Expect = 1.2e-45
Identity = 139/483 (28.78%), Postives = 228/483 (47.20%), Query Frame = 0

Query: 5   LWAIQDLDKKWLLLQKRKKALHLYYRKRFE---EELRRDDDVTRQSLNSQLFESVKKSLE 64
           LW +   D+KW  L+ RK+ L   + K      E++  D D         L  +  + L+
Sbjct: 393 LWRVWQWDEKWTQLRIRKENLTRLFEKMQAYQYEQISADPDKPLADGIRALDTTDMERLK 452

Query: 65  AAESEREVDDVDSKFNLHFPPG-------------------EVGVDEG------------ 124
             +S  E+ DV + F L++                      E G +EG            
Sbjct: 453 DVQSMDELKDVYNHFLLYYGRDIPKMQNAAKASRKKLKRVREEGDEEGEGDEAEDEEQRG 512

Query: 125 -QFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQFGLQLSLEKMRNDELEDPKETPEEMAS 184
            + K+  R+ +Y+IC  AGL  +A KFG + EQFG  L     R++  + P E P E+A 
Sbjct: 513 PELKQASRRDMYTICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAE-PLELAK 572

Query: 185 NFTCAMFESPQAVLKGARHMAAIEISCEPCVRKHVRSYFMDYAVISTNPTSDGNAAIDSF 244
           ++ C+ F +P+AVL+GAR+M A++I+ EP VR+ +R  F + A ++  PT  G   +D  
Sbjct: 573 DYVCSQFPTPEAVLEGARYMVALQIAREPLVRQVLRQTFQERAKLNITPTKKGRKDVDEA 632

Query: 245 HQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLLNVTLKLPEKHLNKLIND------FNE 304
           H     K+L+ KP+    D Q+L I  AE+E LL   + +  K +    ND        +
Sbjct: 633 HYAYSFKYLKNKPVKELRDDQFLKICLAEDEGLLTTDISIDLKGVEGYGNDQTYFEEIKQ 692

Query: 305 YYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARSVMTSKAKKWLLTEYGKNLWN 364
           +Y  D  S   Q WN QR + ++ AL  FL   M KE ++ + ++AK++++    + L+N
Sbjct: 693 FYYRDEFSHQVQEWNRQRTMAIERALQQFLYVQMAKELKNKLLAEAKEYVIKACSRKLYN 752

Query: 365 KISVGPY----QHKENDISSDEEAAP--RVMACCWGPGKPATTF-VMLDSAGEVLDVLYT 424
            + V PY    Q +E+D   DE      RV+   +   +    F  +++  GEV D L  
Sbjct: 753 WLRVAPYRPDQQVEEDDDFMDENQGKGIRVLGIAFSSARDHPVFCALVNGEGEVTDFLRL 812

Query: 425 GSLTLRSQNVNDQQRKK--NDQERVLKFMTDHQPHVVVLGAVNLSCTRLKDDIYEVSAPI 438
              T R     +++R+K   D E + KF+ + +PHVV +   N     L +D+  +   +
Sbjct: 813 PHFTKRRTAWREEEREKKAQDIETLKKFLLNKKPHVVTVAGENRDAQMLIEDVKRIVHEL 872

BLAST of Carg19853 vs. ExPASy Swiss-Prot
Match: Q8UVK2 (Transcription elongation factor SPT6 OS=Danio rerio OX=7955 GN=supt6h PE=1 SV=1)

HSP 1 Score: 179.5 bits (454), Expect = 8.3e-44
Identity = 135/476 (28.36%), Postives = 226/476 (47.48%), Query Frame = 0

Query: 5   LWAIQDLDKKWLLLQKRKKALHLYYRKRFE---EELRRDDDVTRQSLNSQLFESVKKSLE 64
           LW +   D+KW  L+ RK+ L   +++      E++  D D         L  +  + L+
Sbjct: 381 LWKVWQWDEKWTQLKTRKQNLTRLFQRMQSYQFEQISADPDKPLADSTRPLDTADMERLK 440

Query: 65  AAESEREVDDVDSKFNLHFPPG-------------------EVGVDEGQ----------- 124
             +S  E+ DV + F L++                      EV  ++G+           
Sbjct: 441 DVQSIDELGDVYNHFLLYYGRDIPKMQNAAKGGKKKLKKIKEVSEEDGEEAEVEEEEEEE 500

Query: 125 ------FKRPKRKSLYSICSKAGLWEVAGKFGYSSEQFGLQLSLEKMRNDELEDPKETPE 184
                  K+  R+ +YSIC  AGL  +A KFG + EQFG  L     R++  + P E P 
Sbjct: 501 EQKGPDLKQASRRDMYSICQSAGLDGLAKKFGLTPEQFGENLRDSYQRHETEQFPAE-PL 560

Query: 185 EMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKHVRSYFMDYAVISTNPTSDGNAA 244
           E+A ++ C+ F +P+AVL+GAR+M A++I+ EP VR  +R  F + A I+  PT  G   
Sbjct: 561 ELAKDYVCSQFNTPEAVLEGARYMVAMQIAREPLVRHVLRQTFQERAKINIKPTKKGKKD 620

Query: 245 IDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLLNVTLKLPEKHL-----NKLIND 304
           +D  H     K+L+ KP+      Q+L +  AEEE LL + + +    +         ++
Sbjct: 621 VDEAHFAYSFKYLKNKPVKELSGDQFLKMCLAEEEGLLAIDICIDLVGVKGYGDQTYFDE 680

Query: 305 FNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARSVMTSKAKKWLLTEYGKN 364
             ++Y  D  S   Q WN+QR L ++ +L  FL P M KE ++ + ++AK  ++    K 
Sbjct: 681 IKQFYYRDEFSHQVQEWNKQRTLAIERSLQQFLYPQMAKELKNKLIAEAKDNIVKSCCKK 740

Query: 365 LWNKISVGPY----QHKENDISSDEEAAP--RVMACCWGPGKPATTFV-MLDSAGEVLDV 424
           L+N + V PY    Q +E+D   DE      RV+   +  G+    F  +++  GEV+D 
Sbjct: 741 LYNWLKVAPYRPDQQVEEDDDLMDESQGKGIRVLGVAFASGRDTPVFCSLINGEGEVVDF 800

Query: 425 LYTGSLTLR--SQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRLKDDI 428
           L       R  +   +++++K+ D E + KF+   +PHVV +   N     + +DI
Sbjct: 801 LRLPYFLKRRNAWREDEREKKQQDVENLKKFLLSKKPHVVAVSGENRDAHMVMEDI 855

BLAST of Carg19853 vs. ExPASy TrEMBL
Match: A0A6J1EUY6 (Transcription elongation factor spt6 OS=Cucurbita moschata OX=3662 GN=LOC111436171 PE=3 SV=1)

HSP 1 Score: 855.5 bits (2209), Expect = 9.7e-245
Identity = 426/427 (99.77%), Postives = 427/427 (100.00%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA
Sbjct: 390 VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 449

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF
Sbjct: 450 ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 509

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH
Sbjct: 510 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 569

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL
Sbjct: 570 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 629

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
           NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS
Sbjct: 630 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 689

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM
Sbjct: 690 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 749

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL
Sbjct: 750 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 809

Query: 424 KDDIYEV 431
           KDDIYE+
Sbjct: 810 KDDIYEI 816

BLAST of Carg19853 vs. ExPASy TrEMBL
Match: A0A6J1K2G0 (Transcription elongation factor spt6 OS=Cucurbita maxima OX=3661 GN=LOC111491797 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.5e-242
Identity = 424/428 (99.07%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLF+SVKKSLEAA
Sbjct: 387 VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFDSVKKSLEAA 446

Query: 64  ESEREVDDVDSKFNLHFPPGEVG-VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 123
           ESEREVDDVDSKFNLHFP GEVG VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ
Sbjct: 447 ESEREVDDVDSKFNLHFPTGEVGVVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 506

Query: 124 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 183
           FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK
Sbjct: 507 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 566

Query: 184 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 243
           HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL
Sbjct: 567 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 626

Query: 244 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 303
           LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR
Sbjct: 627 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 686

Query: 304 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 363
           SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV
Sbjct: 687 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 746

Query: 364 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 423
           MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR
Sbjct: 747 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 806

Query: 424 LKDDIYEV 431
           LKDDIYE+
Sbjct: 807 LKDDIYEI 814

BLAST of Carg19853 vs. ExPASy TrEMBL
Match: A0A6J1KBH9 (Transcription elongation factor spt6 OS=Cucurbita maxima OX=3661 GN=LOC111491797 PE=3 SV=1)

HSP 1 Score: 846.7 bits (2186), Expect = 4.5e-242
Identity = 424/428 (99.07%), Postives = 426/428 (99.53%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLF+SVKKSLEAA
Sbjct: 369 VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFDSVKKSLEAA 428

Query: 64  ESEREVDDVDSKFNLHFPPGEVG-VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 123
           ESEREVDDVDSKFNLHFP GEVG VDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ
Sbjct: 429 ESEREVDDVDSKFNLHFPTGEVGVVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQ 488

Query: 124 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 183
           FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK
Sbjct: 489 FGLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRK 548

Query: 184 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 243
           HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL
Sbjct: 549 HVRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKL 608

Query: 244 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 303
           LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR
Sbjct: 609 LNVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEAR 668

Query: 304 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 363
           SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV
Sbjct: 669 SVMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFV 728

Query: 364 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 423
           MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR
Sbjct: 729 MLDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTR 788

Query: 424 LKDDIYEV 431
           LKDDIYE+
Sbjct: 789 LKDDIYEI 796

BLAST of Carg19853 vs. ExPASy TrEMBL
Match: A0A5A7TAW1 (Transcription elongation factor spt6 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold35G00920 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 3.9e-230
Identity = 398/427 (93.21%), Postives = 414/427 (96.96%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           +LWAIQDLDKKWLLLQKRKKAL  YY+ R+ EE+R  + VTR +LN QLF+SV +SLEAA
Sbjct: 346 LLWAIQDLDKKWLLLQKRKKALQSYYKNRYLEEIRTAEHVTRTTLNRQLFDSVNRSLEAA 405

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF
Sbjct: 406 ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 465

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH
Sbjct: 466 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 525

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VRSYFMDYAVIST+PT DGN AIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL
Sbjct: 526 VRSYFMDYAVISTSPTPDGNVAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 585

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
           NVTLKLPEKHLNKLI+DFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS
Sbjct: 586 NVTLKLPEKHLNKLISDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 645

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           +MTSKAKKWLL EYGKNLW+K+S+GPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM
Sbjct: 646 LMTSKAKKWLLMEYGKNLWSKVSIGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 705

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL
Sbjct: 706 LDSSGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 765

Query: 424 KDDIYEV 431
           KDDIYE+
Sbjct: 766 KDDIYEI 772

BLAST of Carg19853 vs. ExPASy TrEMBL
Match: A0A5D3DZK9 (Transcription elongation factor spt6 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold2406G00200 PE=3 SV=1)

HSP 1 Score: 807.0 bits (2083), Expect = 3.9e-230
Identity = 398/427 (93.21%), Postives = 414/427 (96.96%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           +LWAIQDLDKKWLLLQKRKKAL  YY+ R+ EE+R  + VTR +LN QLF+SV +SLEAA
Sbjct: 364 LLWAIQDLDKKWLLLQKRKKALQSYYKNRYLEEIRTAEHVTRTTLNRQLFDSVNRSLEAA 423

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF
Sbjct: 424 ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 483

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH
Sbjct: 484 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 543

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VRSYFMDYAVIST+PT DGN AIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL
Sbjct: 544 VRSYFMDYAVISTSPTPDGNVAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 603

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
           NVTLKLPEKHLNKLI+DFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS
Sbjct: 604 NVTLKLPEKHLNKLISDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 663

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           +MTSKAKKWLL EYGKNLW+K+S+GPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM
Sbjct: 664 LMTSKAKKWLLMEYGKNLWSKVSIGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 723

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL
Sbjct: 724 LDSSGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 783

Query: 424 KDDIYEV 431
           KDDIYE+
Sbjct: 784 KDDIYEI 790

BLAST of Carg19853 vs. TAIR 10
Match: AT1G65440.1 (global transcription factor group B1 )

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-192
Identity = 335/427 (78.45%), Postives = 371/427 (86.89%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           V W I DLDKKWLLL+KRK ALH YY KR+EEE RR  D TR +LN  LFESV KSL+ A
Sbjct: 376 VFWMIHDLDKKWLLLRKRKMALHGYYTKRYEEESRRVYDETRLNLNQYLFESVIKSLKVA 435

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           E+EREVDDVDSKFNLHFPPGE+GVDEGQ+KRPKRKS YSICSKAGLWEVA KFGYS+EQ 
Sbjct: 436 ETEREVDDVDSKFNLHFPPGEIGVDEGQYKRPKRKSQYSICSKAGLWEVANKFGYSAEQL 495

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GL LSLEK+  DELED KETPEEMA NF CAMFE+  AVLKGARHMAA+EISCEP V+K+
Sbjct: 496 GLALSLEKL-VDELEDAKETPEEMAKNFVCAMFENSLAVLKGARHMAAVEISCEPSVKKY 555

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VR  +M+ AV+ST+PT+DGN  IDSFHQFS +KWLREKPLS+FE AQWLLIQK EEEKLL
Sbjct: 556 VRGIYMENAVVSTSPTADGNTVIDSFHQFSGIKWLREKPLSKFEGAQWLLIQKGEEEKLL 615

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
            VT KLPE ++N+LI+D NE+YLS GVSK AQLWNEQRKLIL+DAL  FLLPSMEKEARS
Sbjct: 616 QVTFKLPENYMNRLISDCNEHYLSVGVSKYAQLWNEQRKLILEDALHAFLLPSMEKEARS 675

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           ++TS+AK  LL+EYG+ LWNK+S GPYQ KE DI+ DEEAAPRVMACCWGPGKP  TFVM
Sbjct: 676 LLTSRAKSRLLSEYGQALWNKVSAGPYQKKEMDINLDEEAAPRVMACCWGPGKPPNTFVM 735

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GEVLDVLY GSLT RSQNVNDQQRKK+DQ+RVLKFM DHQPHVV LGAVNLSCTRL
Sbjct: 736 LDSSGEVLDVLYAGSLTSRSQNVNDQQRKKSDQDRVLKFMMDHQPHVVALGAVNLSCTRL 795

Query: 424 KDDIYEV 431
           KDDIYEV
Sbjct: 796 KDDIYEV 801

BLAST of Carg19853 vs. TAIR 10
Match: AT1G65440.2 (global transcription factor group B1 )

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-192
Identity = 335/427 (78.45%), Postives = 371/427 (86.89%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           V W I DLDKKWLLL+KRK ALH YY KR+EEE RR  D TR +LN  LFESV KSL+ A
Sbjct: 376 VFWMIHDLDKKWLLLRKRKMALHGYYTKRYEEESRRVYDETRLNLNQYLFESVIKSLKVA 435

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           E+EREVDDVDSKFNLHFPPGE+GVDEGQ+KRPKRKS YSICSKAGLWEVA KFGYS+EQ 
Sbjct: 436 ETEREVDDVDSKFNLHFPPGEIGVDEGQYKRPKRKSQYSICSKAGLWEVANKFGYSAEQL 495

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GL LSLEK+  DELED KETPEEMA NF CAMFE+  AVLKGARHMAA+EISCEP V+K+
Sbjct: 496 GLALSLEKL-VDELEDAKETPEEMAKNFVCAMFENSLAVLKGARHMAAVEISCEPSVKKY 555

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VR  +M+ AV+ST+PT+DGN  IDSFHQFS +KWLREKPLS+FE AQWLLIQK EEEKLL
Sbjct: 556 VRGIYMENAVVSTSPTADGNTVIDSFHQFSGIKWLREKPLSKFEGAQWLLIQKGEEEKLL 615

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
            VT KLPE ++N+LI+D NE+YLS GVSK AQLWNEQRKLIL+DAL  FLLPSMEKEARS
Sbjct: 616 QVTFKLPENYMNRLISDCNEHYLSVGVSKYAQLWNEQRKLILEDALHAFLLPSMEKEARS 675

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           ++TS+AK  LL+EYG+ LWNK+S GPYQ KE DI+ DEEAAPRVMACCWGPGKP  TFVM
Sbjct: 676 LLTSRAKSRLLSEYGQALWNKVSAGPYQKKEMDINLDEEAAPRVMACCWGPGKPPNTFVM 735

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GEVLDVLY GSLT RSQNVNDQQRKK+DQ+RVLKFM DHQPHVV LGAVNLSCTRL
Sbjct: 736 LDSSGEVLDVLYAGSLTSRSQNVNDQQRKKSDQDRVLKFMMDHQPHVVALGAVNLSCTRL 795

Query: 424 KDDIYEV 431
           KDDIYEV
Sbjct: 796 KDDIYEV 801

BLAST of Carg19853 vs. TAIR 10
Match: AT1G65440.3 (global transcription factor group B1 )

HSP 1 Score: 670.2 bits (1728), Expect = 1.1e-192
Identity = 335/427 (78.45%), Postives = 371/427 (86.89%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           V W I DLDKKWLLL+KRK ALH YY KR+EEE RR  D TR +LN  LFESV KSL+ A
Sbjct: 347 VFWMIHDLDKKWLLLRKRKMALHGYYTKRYEEESRRVYDETRLNLNQYLFESVIKSLKVA 406

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           E+EREVDDVDSKFNLHFPPGE+GVDEGQ+KRPKRKS YSICSKAGLWEVA KFGYS+EQ 
Sbjct: 407 ETEREVDDVDSKFNLHFPPGEIGVDEGQYKRPKRKSQYSICSKAGLWEVANKFGYSAEQL 466

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GL LSLEK+  DELED KETPEEMA NF CAMFE+  AVLKGARHMAA+EISCEP V+K+
Sbjct: 467 GLALSLEKL-VDELEDAKETPEEMAKNFVCAMFENSLAVLKGARHMAAVEISCEPSVKKY 526

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VR  +M+ AV+ST+PT+DGN  IDSFHQFS +KWLREKPLS+FE AQWLLIQK EEEKLL
Sbjct: 527 VRGIYMENAVVSTSPTADGNTVIDSFHQFSGIKWLREKPLSKFEGAQWLLIQKGEEEKLL 586

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
            VT KLPE ++N+LI+D NE+YLS GVSK AQLWNEQRKLIL+DAL  FLLPSMEKEARS
Sbjct: 587 QVTFKLPENYMNRLISDCNEHYLSVGVSKYAQLWNEQRKLILEDALHAFLLPSMEKEARS 646

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           ++TS+AK  LL+EYG+ LWNK+S GPYQ KE DI+ DEEAAPRVMACCWGPGKP  TFVM
Sbjct: 647 LLTSRAKSRLLSEYGQALWNKVSAGPYQKKEMDINLDEEAAPRVMACCWGPGKPPNTFVM 706

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GEVLDVLY GSLT RSQNVNDQQRKK+DQ+RVLKFM DHQPHVV LGAVNLSCTRL
Sbjct: 707 LDSSGEVLDVLYAGSLTSRSQNVNDQQRKKSDQDRVLKFMMDHQPHVVALGAVNLSCTRL 766

Query: 424 KDDIYEV 431
           KDDIYEV
Sbjct: 767 KDDIYEV 772

BLAST of Carg19853 vs. TAIR 10
Match: AT1G63210.1 (Transcription elongation factor Spt6 )

HSP 1 Score: 583.9 bits (1504), Expect = 1.0e-166
Identity = 296/426 (69.48%), Postives = 349/426 (81.92%), Query Frame = 0

Query: 4   VLWAIQDLDKKWLLLQKRKKALHLYYRKRFEEELRRDDDVTRQSLNSQLFESVKKSLEAA 63
           VLW IQDLD+KW+LL+KRK AL  YY KRFEEE    D      LN  LFESV KSL+AA
Sbjct: 175 VLWMIQDLDRKWVLLRKRKTALFGYYTKRFEEETCMSD------LNKSLFESVIKSLQAA 234

Query: 64  ESEREVDDVDSKFNLHFPPGEVGVDEGQFKRPKRKSLYSICSKAGLWEVAGKFGYSSEQF 123
           E+EREVDDVDSKFNLHFP  E  +DEGQ+KRP RKS YSICSK G+ E A KFGYS+EQ 
Sbjct: 235 ETEREVDDVDSKFNLHFPHCE--IDEGQYKRPNRKSQYSICSKFGIKEFANKFGYSAEQL 294

Query: 124 GLQLSLEKMRNDELEDPKETPEEMASNFTCAMFESPQAVLKGARHMAAIEISCEPCVRKH 183
           GL LSLEK+  DELED K+TPEEMA N+ CAMF   QAVLKGARHMAA+EISCEP +RK+
Sbjct: 295 GLALSLEKVFVDELEDAKKTPEEMALNYECAMFGDSQAVLKGARHMAAVEISCEPSIRKY 354

Query: 184 VRSYFMDYAVISTNPTSDGNAAIDSFHQFSVVKWLREKPLSRFEDAQWLLIQKAEEEKLL 243
           VR  FM+ AV+ST+PT DGN  IDSFH+FS VKWL EKPL +F+  QWLLIQKAEEEKLL
Sbjct: 355 VRGIFMENAVVSTSPTPDGNVVIDSFHRFSAVKWLSEKPLRKFDGVQWLLIQKAEEEKLL 414

Query: 244 NVTLKLPEKHLNKLINDFNEYYLSDGVSKSAQLWNEQRKLILQDALSGFLLPSMEKEARS 303
            VT KLPE  +N++I++ +E+YLS GVSK AQLWNEQRKLIL+DA+ GF+LPSMEKEARS
Sbjct: 415 QVTFKLPENQMNRIISECSEHYLSVGVSKYAQLWNEQRKLILEDAVHGFVLPSMEKEARS 474

Query: 304 VMTSKAKKWLLTEYGKNLWNKISVGPYQHKENDISSDEEAAPRVMACCWGPGKPATTFVM 363
           ++TS+AK  LL+EYG+ LWNK+S GPY+   N  +S+EEAAPRV+ACCWGPG P TTFVM
Sbjct: 475 LLTSRAKTQLLSEYGQVLWNKVSSGPYRRNNN--TSEEEAAPRVLACCWGPGNPETTFVM 534

Query: 364 LDSAGEVLDVLYTGSLTLRSQNVNDQQRKKNDQERVLKFMTDHQPHVVVLGAVNLSCTRL 423
           LDS+GE++DVLY+GS+ LRS++VNDQ+RKK DQ+R LKF+ DHQP V+ L AVNLSC RL
Sbjct: 535 LDSSGELVDVLYSGSIGLRSRDVNDQKRKKMDQDRFLKFIRDHQPDVLALAAVNLSCVRL 590

Query: 424 KDDIYE 430
           KD+IYE
Sbjct: 595 KDEIYE 590

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7029903.13.4e-252100.00Transcription elongation factor SPT6-like protein, partial [Cucurbita argyrosper... [more]
XP_022929650.12.0e-24499.77transcription elongation factor SPT6 homolog [Cucurbita moschata][more]
XP_023545891.12.2e-24399.06transcription elongation factor SPT6 homolog [Cucurbita pepo subsp. pepo][more]
XP_022996607.19.3e-24299.07transcription elongation factor SPT6 homolog isoform X1 [Cucurbita maxima][more]
XP_022996608.19.3e-24299.07transcription elongation factor SPT6 homolog isoform X2 [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
A8MS851.6e-19178.45Transcription elongation factor SPT6 homolog OS=Arabidopsis thaliana OX=3702 GN=... [more]
Q9CAM11.5e-16569.48Transcription elongation factor SPT6-like OS=Arabidopsis thaliana OX=3702 GN=At1... [more]
Q623836.8e-4628.78Transcription elongation factor SPT6 OS=Mus musculus OX=10090 GN=Supt6h PE=1 SV=... [more]
Q7KZ851.2e-4528.78Transcription elongation factor SPT6 OS=Homo sapiens OX=9606 GN=SUPT6H PE=1 SV=2[more]
Q8UVK28.3e-4428.36Transcription elongation factor SPT6 OS=Danio rerio OX=7955 GN=supt6h PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1EUY69.7e-24599.77Transcription elongation factor spt6 OS=Cucurbita moschata OX=3662 GN=LOC1114361... [more]
A0A6J1K2G04.5e-24299.07Transcription elongation factor spt6 OS=Cucurbita maxima OX=3661 GN=LOC111491797... [more]
A0A6J1KBH94.5e-24299.07Transcription elongation factor spt6 OS=Cucurbita maxima OX=3661 GN=LOC111491797... [more]
A0A5A7TAW13.9e-23093.21Transcription elongation factor spt6 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
A0A5D3DZK93.9e-23093.21Transcription elongation factor spt6 OS=Cucumis melo var. makuwa OX=1194695 GN=E... [more]
Match NameE-valueIdentityDescription
AT1G65440.11.1e-19278.45global transcription factor group B1 [more]
AT1G65440.21.1e-19278.45global transcription factor group B1 [more]
AT1G65440.31.1e-19278.45global transcription factor group B1 [more]
AT1G63210.11.0e-16669.48Transcription elongation factor Spt6 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR023323Tex-like domain superfamilyGENE3D1.10.3500.10coord: 100..344
e-value: 2.2E-74
score: 252.8
IPR037027YqgF/RNase H-like domain superfamilyGENE3D3.30.420.140coord: 345..438
e-value: 1.5E-24
score: 88.6
IPR023319Tex-like protein, HTH domain superfamilyGENE3D1.10.10.650coord: 2..83
e-value: 2.1E-8
score: 36.1
IPR017072Transcription elongation factor Spt6PANTHERPTHR10145TRANSCRIPTION ELONGATION FACTOR SPT6coord: 4..430
NoneNo IPR availablePANTHERPTHR10145:SF7TRANSCRIPTION ELONGATION FACTOR SPT6coord: 4..430
NoneNo IPR availableSUPERFAMILY158832Tex N-terminal region-likecoord: 4..325
IPR012337Ribonuclease H-like superfamilySUPERFAMILY53098Ribonuclease H-likecoord: 345..417

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19853-RACarg19853-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042789 mRNA transcription by RNA polymerase II
biological_process GO:0034728 nucleosome organization
biological_process GO:0032968 positive regulation of transcription elongation from RNA polymerase II promoter
biological_process GO:0050684 regulation of mRNA processing
biological_process GO:0006414 translational elongation
biological_process GO:0006139 nucleobase-containing compound metabolic process
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0008023 transcription elongation factor complex
molecular_function GO:0003677 DNA binding
molecular_function GO:0042393 histone binding
molecular_function GO:0031491 nucleosome binding
molecular_function GO:0003746 translation elongation factor activity