Carg19839 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19839
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Ubiquitin carboxyl-terminal hydrolase 15
Location	Carg_Chr05: 4888016 .. 4888875 (-)
RNA-Seq Expression	Carg19839
Synteny	Carg19839

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATATATTTCCCCTCTTCTTCTTCACCCTTTTCCCACCTTCAAATGAAATTCAACCCTTCAAATCCAAGCATTTCAAAGTTGTTCTTGAACTTCATGAACAAATGAAAAACACCCCATGAAACAAAATCTTCACACCATTTCTCTCACGATGTCGCCTTATGGGGTTCTATTTTCGCTCATTTTCCTGCTCTTTCTCGCCCATAACGCCATTGAAGCGACCTCAATCCACGTGAGTACTCAAAATCCGCTTCTCCAAAGAACCCACATCTCAAAAAATCGAAACCCCATTGGGTTCCCATTTCCCCCAAATCGATTGGTTCAATCCATTTTCCTGCTCTGATTCTGTGTGCTTCTTGTTTCTTAGGTTAATGACTCCGACCAAGAACCGATGATGGTTCCATTGATGGAGAAGATGGAAACGACGGCGATGGTGATAAACAGCGGCCGACGGCAGCTTAGGAGCTTCCAGATTTGTGCTCTCTGCACCTGCTGCGGCGGTGCCAGAGGAATGTGCGTTCCATCACCCTGTTGCTATGCTATAAACTGTAACATCCCTAATAGGCCATTTGGGTTTTGCTCTTTTACACCAAGGACCTGTAACTGCTTTGGCTGCCATATTTAATAAGCTTTCTTGTTATGATATTGACAATGGTTCATTGTTGGATCTCTGAAACACTGTGGAATATATGTTCTAAATTGTTCATTGTTCATGGAATTGTGTCTTGATTTGGTGTCTGTAGGAGATATCATAGCATGGTGCCATAGTTGAGCTTTTGAGGCCAGAATGTACCAAGATTTGGCTTGTTGGGCACTGTTGATACCTCTATTACGATGATCTTTGTGCCTTTTTTTTTTTTTT

mRNA sequence

CATATATTTCCCCTCTTCTTCTTCACCCTTTTCCCACCTTCAAATGAAATTCAACCCTTCAAATCCAAGCATTTCAAAGTTGTTCTTGAACTTCATGAACAAATGAAAAACACCCCATGAAACAAAATCTTCACACCATTTCTCTCACGATGTCGCCTTATGGGGTTCTATTTTCGCTCATTTTCCTGCTCTTTCTCGCCCATAACGCCATTGAAGCGACCTCAATCCACGTTAATGACTCCGACCAAGAACCGATGATGGTTCCATTGATGGAGAAGATGGAAACGACGGCGATGGTGATAAACAGCGGCCGACGGCAGCTTAGGAGCTTCCAGATTTGTGCTCTCTGCACCTGCTGCGGCGGTGCCAGAGGAATGTGCGTTCCATCACCCTGTTGCTATGCTATAAACTGTAACATCCCTAATAGGCCATTTGGGTTTTGCTCTTTTACACCAAGGACCTGTAACTGCTTTGGCTGCCATATTTAATAAGCTTTCTTGTTATGATATTGACAATGGTTCATTGTTGGATCTCTGAAACACTGTGGAATATATGTTCTAAATTGTTCATTGTTCATGGAATTGTGTCTTGATTTGGTGTCTGTAGGAGATATCATAGCATGGTGCCATAGTTGAGCTTTTGAGGCCAGAATGTACCAAGATTTGGCTTGTTGGGCACTGTTGATACCTCTATTACGATGATCTTTGTGCCTTTTTTTTTTTTTT

Coding sequence (CDS)

ATGAAACAAAATCTTCACACCATTTCTCTCACGATGTCGCCTTATGGGGTTCTATTTTCGCTCATTTTCCTGCTCTTTCTCGCCCATAACGCCATTGAAGCGACCTCAATCCACGTTAATGACTCCGACCAAGAACCGATGATGGTTCCATTGATGGAGAAGATGGAAACGACGGCGATGGTGATAAACAGCGGCCGACGGCAGCTTAGGAGCTTCCAGATTTGTGCTCTCTGCACCTGCTGCGGCGGTGCCAGAGGAATGTGCGTTCCATCACCCTGTTGCTATGCTATAAACTGTAACATCCCTAATAGGCCATTTGGGTTTTGCTCTTTTACACCAAGGACCTGTAACTGCTTTGGCTGCCATATTTAA

Protein sequence

MKQNLHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINSGRRQLRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI

Homology

[Close]

BLAST of Carg19839 vs. NCBI nr
Match: KAG7029889.1 (hypothetical protein SDJN02_08232 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 260.8 bits (665), Expect = 6.1e-66
Identity = 123/123 (100.00%), Postives = 123/123 (100.00%), Query Frame = 0


Query: 1   MKQNLHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAM 60
           MKQNLHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAM
Sbjct: 1   MKQNLHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAM 60

Query: 61  VINSGRRQLRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFG 120
           VINSGRRQLRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFG
Sbjct: 61  VINSGRRQLRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFG 120

Query: 121 CHI 124
           CHI
Sbjct: 121 CHI 123

[Close]

BLAST of Carg19839 vs. NCBI nr
Match: XP_023546744.1 (uncharacterized protein LOC111805758 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 255.8 bits (652), Expect = 2.0e-64
Identity = 120/123 (97.56%), Postives = 122/123 (99.19%), Query Frame = 0


Query: 1   MKQNLHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAM 60
           MKQ+LHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAM
Sbjct: 1   MKQSLHTISLTMSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAM 60

Query: 61  VINSGRRQLRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFG 120
           VIN GRRQLRSFQICALCTCCGGARG+CVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFG
Sbjct: 61  VINGGRRQLRSFQICALCTCCGGARGVCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFG 120

Query: 121 CHI 124
           CHI
Sbjct: 121 CHI 123

[Close]

BLAST of Carg19839 vs. NCBI nr
Match: KAG6598934.1 (hypothetical protein SDJN03_08712, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 239.6 bits (610), Expect = 1.4e-59
Identity = 112/112 (100.00%), Postives = 112/112 (100.00%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINSGRRQLRS 71
           MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINSGRRQLRS
Sbjct: 1   MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINSGRRQLRS 60

Query: 72  FQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           FQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI
Sbjct: 61  FQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 112

[Close]

BLAST of Carg19839 vs. NCBI nr
Match: KGN61259.1 (hypothetical protein Csa_005922 [Cucumis sativus])

HSP 1 Score: 207.6 bits (527), Expect = 6.1e-50
Identity = 97/113 (85.84%), Postives = 104/113 (92.04%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINS-GRRQLR 71
           MS YGVLFS I LLFLA NAIE TSIHVN+SDQEP+MVPLME   TTAM++NS GRRQL+
Sbjct: 1   MSSYGVLFSFILLLFLAQNAIEGTSIHVNESDQEPIMVPLMETTTTTAMMMNSGGRRQLK 60

Query: 72  SFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           SFQICALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 61  SFQICALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 113

[Close]

BLAST of Carg19839 vs. NCBI nr
Match: XP_022965498.1 (uncharacterized protein LOC111465383 [Cucurbita maxima])

HSP 1 Score: 201.4 bits (511), Expect = 4.4e-48
Identity = 95/114 (83.33%), Postives = 104/114 (91.23%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVND-SDQEPMMVPLMEKMET-TAMVINSGRRQL 71
           MSPYGV+F LIF+LFLAH+AIEATS H N  SDQEPMMVPLME +ET TA+ +N  RRQL
Sbjct: 1   MSPYGVVFRLIFVLFLAHDAIEATSNHGNRWSDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 72  RSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           RSFQ+CALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg19839 vs. ExPASy TrEMBL
Match: A0A0A0LJJ1 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G074210 PE=4 SV=1)

HSP 1 Score: 207.6 bits (527), Expect = 3.0e-50
Identity = 97/113 (85.84%), Postives = 104/113 (92.04%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINS-GRRQLR 71
           MS YGVLFS I LLFLA NAIE TSIHVN+SDQEP+MVPLME   TTAM++NS GRRQL+
Sbjct: 1   MSSYGVLFSFILLLFLAQNAIEGTSIHVNESDQEPIMVPLMETTTTTAMMMNSGGRRQLK 60

Query: 72  SFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           SFQICALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 61  SFQICALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 113

[Close]

BLAST of Carg19839 vs. ExPASy TrEMBL
Match: A0A6J1HP40 (uncharacterized protein LOC111465383 OS=Cucurbita maxima OX=3661 GN=LOC111465383 PE=4 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 2.1e-48
Identity = 95/114 (83.33%), Postives = 104/114 (91.23%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVND-SDQEPMMVPLMEKMET-TAMVINSGRRQL 71
           MSPYGV+F LIF+LFLAH+AIEATS H N  SDQEPMMVPLME +ET TA+ +N  RRQL
Sbjct: 1   MSPYGVVFRLIFVLFLAHDAIEATSNHGNRWSDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 72  RSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           RSFQ+CALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg19839 vs. ExPASy TrEMBL
Match: A0A5D3C6S7 (Ubiquitin carboxyl-terminal hydrolase 15 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold105G00010 PE=4 SV=1)

HSP 1 Score: 199.1 bits (505), Expect = 1.1e-47
Identity = 92/112 (82.14%), Postives = 99/112 (88.39%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLMEKMETTAMVINSGRRQLRS 71
           MS YGVLFS IFLL LA NAIE TSI VN+SDQEP+MVPLME   T  M+ + GRRQL+S
Sbjct: 1   MSSYGVLFSFIFLLILAQNAIEGTSILVNESDQEPIMVPLMETTTTAMMMNSGGRRQLKS 60

Query: 72  FQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           FQICALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 61  FQICALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 112

[Close]

BLAST of Carg19839 vs. ExPASy TrEMBL
Match: A0A6J1D9H7 (uncharacterized protein LOC111018147 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111018147 PE=4 SV=1)

HSP 1 Score: 198.4 bits (503), Expect = 1.8e-47
Identity = 95/115 (82.61%), Postives = 105/115 (91.30%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVNDSDQEPMMVPLM---EKMETTAMVINSGRRQ 71
           M  +GV FSLIF+LFLA NAIEATSI+VN SDQEPMMVPLM   EKME TA+V++ GRR+
Sbjct: 2   MPSHGVAFSLIFVLFLAQNAIEATSIYVNVSDQEPMMVPLMGLGEKME-TAVVMSGGRRR 61

Query: 72  LRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           LRSFQ+CALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 62  LRSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 115

[Close]

BLAST of Carg19839 vs. ExPASy TrEMBL
Match: A0A6J1FHK2 (uncharacterized protein LOC111444031 OS=Cucurbita moschata OX=3662 GN=LOC111444031 PE=4 SV=1)

HSP 1 Score: 193.4 bits (490), Expect = 5.8e-46
Identity = 92/114 (80.70%), Postives = 101/114 (88.60%), Query Frame = 0


Query: 12  MSPYGVLFSLIFLLFLAHNAIEATSIHVND-SDQEPMMVPLMEKMET-TAMVINSGRRQL 71
           M  YGV+F LIF+LFLAH+AIEATS H N   DQEPMMVPLME +ET TA+ +N  RRQL
Sbjct: 1   MLRYGVVFRLIFVLFLAHDAIEATSNHGNRWFDQEPMMVPLMENVETMTAVAMNGNRRQL 60

Query: 72  RSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           RSFQ+CALCTCCGGARGMC+PSPCCYAINCNIPNRPFGFCSFTP+TCNCFGCHI
Sbjct: 61  RSFQVCALCTCCGGARGMCLPSPCCYAINCNIPNRPFGFCSFTPKTCNCFGCHI 114

[Close]

BLAST of Carg19839 vs. TAIR 10
Match: AT1G65295.1 (unknown protein; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT5G01015.1); Has 90 Blast hits to 90 proteins in 15 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 90; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 143.3 bits (360), Expect = 1.3e-34
Identity = 67/109 (61.47%), Postives = 83/109 (76.15%), Query Frame = 0


Query: 20  SLIFLLFLAHNAIEATSIHVN-----DSDQEPMMVPLMEKMETTAMVINSGRRQLRSFQI 79
           S++FLL L  +   ++S   N     + D E MMVPL E+     MV+N  RR+L SFQI
Sbjct: 10  SIVFLLLLLFSIFISSSHQANEVLPAEGDNEMMMVPLGEE---KFMVMNETRRKLGSFQI 69

Query: 80  CALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           C++CTCCGGA+G+C+PSPCCYAINCNIPNRPFG+CSFTP+TCNCFGCHI
Sbjct: 70  CSVCTCCGGAKGLCLPSPCCYAINCNIPNRPFGYCSFTPKTCNCFGCHI 115

[Close]

BLAST of Carg19839 vs. TAIR 10
Match: AT5G01015.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: endomembrane system; EXPRESSED IN: 23 plant structures; EXPRESSED DURING: 13 growth stages; BEST Arabidopsis thaliana protein match is: unknown protein (TAIR:AT1G65295.1); Has 30201 Blast hits to 17322 proteins in 780 species: Archae - 12; Bacteria - 1396; Metazoa - 17338; Fungi - 3422; Plants - 5037; Viruses - 0; Other Eukaryotes - 2996 (source: NCBI BLink). )

HSP 1 Score: 65.5 bits (158), Expect = 3.5e-11
Identity = 24/58 (41.38%), Postives = 35/58 (60.34%), Query Frame = 0


Query: 66  RRQLRSFQICALCTCCGGARGMCVPSPCCYAINCNIPNRPFGFCSFTPRTCNCFGCHI 124
           R+ +   ++C  C CC      C   PCC+ INC +PN+PFG C+F P++C+C  C I
Sbjct: 49  RKLMTPLELCLECKCCSST--TCATMPCCFGINCQLPNKPFGVCAFVPKSCHCNSCSI 104

The following BLAST results are available for this feature:

BLAST of Carg19839 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7029889.1	6.1e-66	100.00	hypothetical protein SDJN02_08232 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023546744.1	2.0e-64	97.56	uncharacterized protein LOC111805758 [Cucurbita pepo subsp. pepo]	[more]
KAG6598934.1	1.4e-59	100.00	hypothetical protein SDJN03_08712, partial [Cucurbita argyrosperma subsp. sorori...	[more]
KGN61259.1	6.1e-50	85.84	hypothetical protein Csa_005922 [Cucumis sativus]	[more]
XP_022965498.1	4.4e-48	83.33	uncharacterized protein LOC111465383 [Cucurbita maxima]	[more]

BLAST of Carg19839 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg19839 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A0A0LJJ1	3.0e-50	85.84	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G074210 PE=4 SV=1	[more]
A0A6J1HP40	2.1e-48	83.33	uncharacterized protein LOC111465383 OS=Cucurbita maxima OX=3661 GN=LOC111465383...	[more]
A0A5D3C6S7	1.1e-47	82.14	Ubiquitin carboxyl-terminal hydrolase 15 OS=Cucumis melo var. makuwa OX=1194695 ...	[more]
A0A6J1D9H7	1.8e-47	82.61	uncharacterized protein LOC111018147 isoform X1 OS=Momordica charantia OX=3673 G...	[more]
A0A6J1FHK2	5.8e-46	80.70	uncharacterized protein LOC111444031 OS=Cucurbita moschata OX=3662 GN=LOC1114440...	[more]

BLAST of Carg19839 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G65295.1	1.3e-34	61.47	unknown protein; LOCATED IN: endomembrane system; EXPRESSED IN: 22 plant structu...	[more]
AT5G01015.1	3.5e-11	41.38	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PANTHER	PTHR33786:SF2	UBIQUITIN CARBOXYL-TERMINAL HYDROLASE	coord: 13..123
None	No IPR available	PANTHER	PTHR33786	FAMILY NOT NAMED	coord: 13..123

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19839-RA	Carg19839-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016787	hydrolase activity