Carg19689 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19689
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	pre-mRNA-splicing factor CWC25-like isoform X2
Location	Carg_Chr16: 1767266 .. 1767475 (+)
RNA-Seq Expression	Carg19689
Synteny	Carg19689

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTACCAAAAGGGATGAGATGAAGAGAGGTGGTGGTGGGAACTACAGGGGAAGAGACAGGTTTAGTGAGAGGAAAGGATATCGAGCTGGTGGTAGTACCCGTGTGGAGAAACGGAAGCACGACCTATTTCACGAGGCTAATCGGAGTCCAACCCCGAAGAATGAAGAGGACCAAATTTCCAAGGTTGAAGCTCTTCTTGCTTCCTAG

mRNA sequence

ATGGCTACCAAAAGGGATGAGATGAAGAGAGGTGGTGGTGGGAACTACAGGGGAAGAGACAGGTTTAGTGAGAGGAAAGGATATCGAGCTGGTGGTAGTACCCGTGTGGAGAAACGGAAGCACGACCTATTTCACGAGGCTAATCGGAGTCCAACCCCGAAGAATGAAGAGGACCAAATTTCCAAGGTTGAAGCTCTTCTTGCTTCCTAG

Coding sequence (CDS)

ATGGCTACCAAAAGGGATGAGATGAAGAGAGGTGGTGGTGGGAACTACAGGGGAAGAGACAGGTTTAGTGAGAGGAAAGGATATCGAGCTGGTGGTAGTACCCGTGTGGAGAAACGGAAGCACGACCTATTTCACGAGGCTAATCGGAGTCCAACCCCGAAGAATGAAGAGGACCAAATTTCCAAGGTTGAAGCTCTTCTTGCTTCCTAG

Protein sequence

MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQISKVEALLAS

Homology

[Close]

BLAST of Carg19689 vs. NCBI nr
Match: KAG7014940.1 (hypothetical protein SDJN02_22571, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 134.8 bits (338), Expect = 2.8e-28
Identity = 69/69 (100.00%), Postives = 69/69 (100.00%), Query Frame = 0


Query: 1  MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60
          MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI
Sbjct: 1  MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60

Query: 61 SKVEALLAS 70
          SKVEALLAS
Sbjct: 61 SKVEALLAS 69

[Close]

BLAST of Carg19689 vs. NCBI nr
Match: KAG6576913.1 (hypothetical protein SDJN03_24487, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 123.2 bits (308), Expect = 8.5e-25
Identity = 62/62 (100.00%), Postives = 62/62 (100.00%), Query Frame = 0


Query: 1  MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60
          MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI
Sbjct: 1  MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60

Query: 61 SK 63
          SK
Sbjct: 61 SK 62

[Close]

BLAST of Carg19689 vs. NCBI nr
Match: XP_022942672.1 (pre-mRNA-splicing factor CWC25-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 116.7 bits (291), Expect = 7.9e-23
Identity = 60/69 (86.96%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 1   MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60
           MA+KRDEMKRGGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEANRSPTPKNEEDQI
Sbjct: 258 MASKRDEMKRGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEANRSPTPKNEEDQI 317

Query: 61  SKVEALLAS 70
           +KVEALLAS
Sbjct: 318 AKVEALLAS 326

[Close]

BLAST of Carg19689 vs. NCBI nr
Match: XP_022984554.1 (pre-mRNA-splicing factor CWC25 isoform X2 [Cucurbita maxima])

HSP 1 Score: 113.6 bits (283), Expect = 6.7e-22
Identity = 59/69 (85.51%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 1   MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60
           MA+KRDEMKRGGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEANRSPTPKNEE QI
Sbjct: 258 MASKRDEMKRGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEANRSPTPKNEEAQI 317

Query: 61  SKVEALLAS 70
           +KVEALLAS
Sbjct: 318 AKVEALLAS 326

[Close]

BLAST of Carg19689 vs. NCBI nr
Match: XP_022942670.1 (pre-mRNA-splicing factor CWC25-like isoform X1 [Cucurbita moschata] >XP_022942671.1 pre-mRNA-splicing factor CWC25-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 107.8 bits (268), Expect = 3.7e-20
Identity = 60/81 (74.07%), Postives = 63/81 (77.78%), Query Frame = 0


Query: 1   MATKRDEMKR------------GGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEAN 60
           MA+KRDEMKR            GGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEAN
Sbjct: 258 MASKRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEAN 317

Query: 61  RSPTPKNEEDQISKVEALLAS 70
           RSPTPKNEEDQI+KVEALLAS
Sbjct: 318 RSPTPKNEEDQIAKVEALLAS 338

[Close]

BLAST of Carg19689 vs. ExPASy TrEMBL
Match: A0A6J1FQX4 (pre-mRNA-splicing factor CWC25-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111447638 PE=4 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 3.8e-23
Identity = 60/69 (86.96%), Postives = 63/69 (91.30%), Query Frame = 0


Query: 1   MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60
           MA+KRDEMKRGGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEANRSPTPKNEEDQI
Sbjct: 258 MASKRDEMKRGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEANRSPTPKNEEDQI 317

Query: 61  SKVEALLAS 70
           +KVEALLAS
Sbjct: 318 AKVEALLAS 326

[Close]

BLAST of Carg19689 vs. ExPASy TrEMBL
Match: A0A6J1JAV5 (pre-mRNA-splicing factor CWC25 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111482807 PE=4 SV=1)

HSP 1 Score: 113.6 bits (283), Expect = 3.3e-22
Identity = 59/69 (85.51%), Postives = 62/69 (89.86%), Query Frame = 0


Query: 1   MATKRDEMKRGGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEANRSPTPKNEEDQI 60
           MA+KRDEMKRGGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEANRSPTPKNEE QI
Sbjct: 258 MASKRDEMKRGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEANRSPTPKNEEAQI 317

Query: 61  SKVEALLAS 70
           +KVEALLAS
Sbjct: 318 AKVEALLAS 326

[Close]

BLAST of Carg19689 vs. ExPASy TrEMBL
Match: A0A6J1FPJ0 (pre-mRNA-splicing factor CWC25-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111447638 PE=4 SV=1)

HSP 1 Score: 107.8 bits (268), Expect = 1.8e-20
Identity = 60/81 (74.07%), Postives = 63/81 (77.78%), Query Frame = 0


Query: 1   MATKRDEMKR------------GGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEAN 60
           MA+KRDEMKR            GGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEAN
Sbjct: 258 MASKRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEAN 317

Query: 61  RSPTPKNEEDQISKVEALLAS 70
           RSPTPKNEEDQI+KVEALLAS
Sbjct: 318 RSPTPKNEEDQIAKVEALLAS 338

[Close]

BLAST of Carg19689 vs. ExPASy TrEMBL
Match: A0A6J1D6L3 (splicing factor U2af small subunit B OS=Momordica charantia OX=3673 GN=LOC111017739 PE=4 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-19
Identity = 60/81 (74.07%), Postives = 63/81 (77.78%), Query Frame = 0


Query: 1   MATKRDEMKR-----------GGGGNYRGRDRFSERKGYRAG-GSTRVEKRKHDLFHEAN 60
           M  KRDE+KR           GGGGNYRGRDRF  R+GYRAG G TRVEK KHDLFHEAN
Sbjct: 90  MGAKRDEIKRGGYPVRERYGNGGGGNYRGRDRFGGRQGYRAGAGGTRVEKWKHDLFHEAN 149

Query: 61  RSPTPKNEEDQISKVEALLAS 70
           RSPTPKNEEDQI+KVEALLAS
Sbjct: 150 RSPTPKNEEDQIAKVEALLAS 170

[Close]

BLAST of Carg19689 vs. ExPASy TrEMBL
Match: A0A6J1J5K1 (pre-mRNA-splicing factor CWC25 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111482807 PE=4 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.5e-19
Identity = 59/81 (72.84%), Postives = 62/81 (76.54%), Query Frame = 0


Query: 1   MATKRDEMKR------------GGGGNYRGRDRFSERKGYRAGGSTRVEKRKHDLFHEAN 60
           MA+KRDEMKR            GGGGNYRGRDRF  R+GY A G TRVEK KHDLFHEAN
Sbjct: 258 MASKRDEMKRGGYSVRERYGNGGGGGNYRGRDRFGGRQGYPASGGTRVEKWKHDLFHEAN 317

Query: 61  RSPTPKNEEDQISKVEALLAS 70
           RSPTPKNEE QI+KVEALLAS
Sbjct: 318 RSPTPKNEEAQIAKVEALLAS 338

[Close]

BLAST of Carg19689 vs. TAIR 10
Match: AT3G19650.1 (cyclin-related )

HSP 1 Score: 46.6 bits (109), Expect = 9.4e-06
Identity = 36/68 (52.94%), Postives = 45/68 (66.18%), Query Frame = 0


Query: 6   DEMKRGGGGNYRG-RDRF--SERKGYRAGGSTRVEKR-KHDLFHEANRSPTPKNEEDQIS 65
           DE   G   +Y+  RDRF  + R G+ +G  TR EK+  HDLF EAN+SP    EE+QI+
Sbjct: 236 DERGAGSRQSYQADRDRFNGNGRSGF-SGSWTRNEKKWDHDLFDEANKSPAKATEEEQIA 295

Query: 66  KVEALLAS 70
           KVEALLAS
Sbjct: 296 KVEALLAS 302

The following BLAST results are available for this feature:

BLAST of Carg19689 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014940.1	2.8e-28	100.00	hypothetical protein SDJN02_22571, partial [Cucurbita argyrosperma subsp. argyro...	[more]
KAG6576913.1	8.5e-25	100.00	hypothetical protein SDJN03_24487, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022942672.1	7.9e-23	86.96	pre-mRNA-splicing factor CWC25-like isoform X2 [Cucurbita moschata]	[more]
XP_022984554.1	6.7e-22	85.51	pre-mRNA-splicing factor CWC25 isoform X2 [Cucurbita maxima]	[more]
XP_022942670.1	3.7e-20	74.07	pre-mRNA-splicing factor CWC25-like isoform X1 [Cucurbita moschata] >XP_02294267...	[more]

BLAST of Carg19689 vs. NCBI nr


Match Name	E-value	Identity	Description

BLAST of Carg19689 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1FQX4	3.8e-23	86.96	pre-mRNA-splicing factor CWC25-like isoform X2 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1JAV5	3.3e-22	85.51	pre-mRNA-splicing factor CWC25 isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]
A0A6J1FPJ0	1.8e-20	74.07	pre-mRNA-splicing factor CWC25-like isoform X1 OS=Cucurbita moschata OX=3662 GN=...	[more]
A0A6J1D6L3	1.2e-19	74.07	splicing factor U2af small subunit B OS=Momordica charantia OX=3673 GN=LOC111017...	[more]
A0A6J1J5K1	1.5e-19	72.84	pre-mRNA-splicing factor CWC25 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC1114...	[more]

BLAST of Carg19689 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G19650.1	9.4e-06	52.94	cyclin-related	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..61
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 1..69
None	No IPR available	PANTHER	PTHR36364	OS03G0203000 PROTEIN	coord: 11..69

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19689-RA	Carg19689-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006397	mRNA processing
biological_process	GO:0051028	mRNA transport
biological_process	GO:0000184	nuclear-transcribed mRNA catabolic process, nonsense-mediated decay
biological_process	GO:0006417	regulation of translation
biological_process	GO:0008380	RNA splicing
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0035145	exon-exon junction complex
molecular_function	GO:0003729	mRNA binding