Carg19686 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19686
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Pectin lyase-like superfamily protein
Location	Carg_Chr16: 1776410 .. 1779834 (-)
RNA-Seq Expression	Carg19686
Synteny	Carg19686

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAGAGCAAAAGAGTTGCTTTTGTGGCGATACTGATGATAATGGTTATGGTAGGTTTAGCCTCGGCGGCCTGCACGAACGCGATCAAGCTCGTGAGCGACGGTGAAAATGAGATGGAAAGTGGGTTTGGAAGCAAGATGATGAAAAATGAGTTTCCAAGCCCAATCGATAGGTGTGGTCTTGTACGAATAAGATGCGATTCTGATTGCCACTGCCCTTCACCCTGCGTTTGCTTGCAAGGAACCTGCACCTGAAAATATCCATCCATTTCATATGCCTCCGACCCCGACCCCGACCCCGATCCCGACTCCTACTACTATTACTATTACTATTACTACTACTACGCTTTGTGTGTTTTGACTTTCCTTTTCTTAAGTTCTCTATAATTATAATAAATCTCTCGTTGGTCGACCGCTCTCTGTTTAAGTGATCGGTTTATGTAGTAAAAATCTTACGATCTATAAATCAATAAAGTTATTTTGTTACAGTATGCTTTGTTCTCACTTACGTGCATTGGGTGTGTGATATCATTTGTAACCGTCTATACCCATCACTAGCAGATATTGTCTGTTTTGGTTTGTTACGTATCGTTGTCAGTCTCACAGTTTTAAAACATGTTTTTTACTAAGAGGTTCCACGCCAGAAATGTTTCATTCCCCTCTTTAACCAACGTGGGATCTCACAATCTACTCGTTTTGGGCCAACGTCCTCACCGGCACAATGCTCAATATATACCATTTGTAACCATCTAAGAAGAAAATTTCACAATGATACCATTTATAACCTCGCATTGTTTGACCCGAGAAATGAAAAATAAGAAAATAACTTCTTAATATGGCTAATTAAACTAAGATTTAAAATAAATTAGCGTTACTTCAATATTTCTACGTTCAAAATAAATTAGAATTTTTGTCCGATCCAAAATCTAGATTTTACAGGCGATTGTTACCTCATTTCCTCCGATATAAATAACCATATTTATTATTTGTCTGGAACAATTTTTAAGAATGAAGAACATATAGAACAACATGTTACGAACAATCATTTTTATCTGCTCGAAAATCGATCACCAATGATCCCAAAGAAATCACATGATGGGTCGAAAAATTAGTTGTTACGAACGATTTTTATTCATATAAATAATTAACTAGAGGATTATTAATCTTGATGAACTTACCAAAAAAAATTAATCAAATCCAAATTTGGAGTAAATTGATTCATATTTAATACACCAAAACTATTAATTTCTAAAAAAATTGTTTCCATCAAAAGATTTATGAACATACTAAAATTAGAAGACCCATTAATTTGTCACCTAAAATAAAATGTCCCCAACATTTGCTTTCTTTAAGCCTATAATTTCCATACTTCCAAAATATGGGTTTTTTCCCTTTATAAAATAATTAATTAATTTCCCATTTTACCCCTAATGCTTTGTTTATAAACTCGACTCGATATCTCCATTCATATCAGACATTTTGGGGTCTTCAAATCTTCTCTCGTTCTTGCTTTCGTATCGTCAGATTAGAAATGATGAAGCTATTTTCTGCTTTAGTTTTTATGTTTTCCACAGTCATTTTAAGCTCGTGGATTGTATCAGCTTCATTTGATTTTAATGTTCTTGATTATGGAGCTGTTGGAAATGGAAAAACGGATGATTCACAAGTAATTGCTTTGATTCGGTTTTTGTTTATGTACGTATAGTTCCGTCTTTCTTATTAGTCTTTCTCGATCTCTTTTTGTGGTGATAAGGCTTTTTTGAAAGCATGGAAAGACGTGTGTCGGGCAACGATAGGCATTCCAACATTGCGTGTACCAATTGGAAAAACTTACTTGTTGAAGCCTCTCAAATTCGAGGGTCCTTGTAACCCTAAACGAGTACATTTTGAGGTAACTATTGCTACTAAAATTGCCTTCGTAATTGTAAGATTTCACATCGGTTGGAGAGAGGAACGAAACATTCCTTACAAGGGTGTAGAAACCTCTCTTTAGTAGACGTGTTTTAAAACCGCAAAGTTGACAACTATACATAACGAGTCAAAACAGACGATAGTTACTAGTAGTGAGCTTGGACTGATACACTGTTGTTGTTTCATAGGTTAGAGGAACTATCATGGCTCCGAGCGTGGCTGCATGGCGTAGCCATAACGAAGAAAACTGGATCCAATTCTTGGACATAGCGGGTCTCAATATCAATGGAGCAGGTCAATTCGATGGCCAGAGTTCTTCTTGGTGGAAAGATTGCCATTCAAACTGCACACCTCCAACGGTAAGTTTTTAACGATCTAATTCTAGGGTTTGATCGTTAAACTGATAACGTGAATATTAATTATATTATATGGTTTTATAGGCATTATTTTTCCAAAATTGCACCGGGCTCCGACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCATAAACGCTTGCAATGATGTCGTTATCTCAAACGTTCACATCATTGCTCCCGAAAACATCCCCAACACATACGGGTTCAACATTTCTAGATCAAACAATGTTCTTATTCAAAACTCCTTAATCGAAACAGGTATCAAATTTGTATTACTTCGTTTTCAAACTGACTTAGAATTTCATAATGGCTTTCTAATTTTATCGTAGGTGACGAATGTATTTCTCTATACAACGGTTCTTCTAATATCAATATCGTCGGTGTTACATGTGGACCTGGTTACGGTATAAGGTACGAAAAACGACATTTTCCATATTATAATTTAATTGTTTTTGTATTAATTTATAATTATTATCTTAATTTCTATAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATCGAAAACATCAATGTAAGCAATTGCACCCTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTACAAAGGTTAATTATAAGTAGTTTTTAGTTTATTCATATACAATTTTTTTAATATATATTACTAAAAAATGACGGGTTTTCTTGACGTAATTACAGGGTGGACATGGATATGTAAGAAACGTCGTATACAAAAAAATAACGCTAATTGATACCCAATTGCCGATCGTTATTGATCAACAGTATTCAACTTTTGCCAACACGAGAAAAATTAAGGTATATATCAAATGAAATTTTATCTATTTTTGTGTACATATGATGTTAAATAGAATCTATAACTTGTATGTTGGTTTGTTTTCAGGAAATGGGTATAAAAGTGATGGATGTGACGTATCGTGAAATTCATGGAATATCAACAAATGAATCGACAGTCATTTTGAATTGTTCTCAAACTCGTTGTAGTAATATTGTAATGGAAGACGTAAGCATACGGACGTCATCTCCAAAAAAACAAGTCAAAGCGTTTTGCCAAAATGTTGATGGAAGAGCCGTATCTACCGTCCCAATCGTGTCTTGTTTATCGAAATCTAATTAA

mRNA sequence

ATGGAGAGCAAAAGAGTTGCTTTTGTGGCGATACTGATGATAATGGTTATGGTAGGTTTAGCCTCGGCGGCCTGCACGAACGCGATCAAGCTCGTGAGCGACGGTGAAAATGAGATGGAAAGTGGGTTTGGAAGCAAGATGATGAAAAATGAGTTTCCAAGCCCAATCGATAGATTAGAAATGATGAAGCTATTTTCTGCTTTAGTTTTTATGTTTTCCACAGTCATTTTAAGCTCGTGGATTGTATCAGCTTCATTTGATTTTAATGTTCTTGATTATGGAGCTGTTGGAAATGGAAAAACGGATGATTCACAAGCTTTTTTGAAAGCATGGAAAGACGTGTGTCGGGCAACGATAGGCATTCCAACATTGCGTGTACCAATTGGAAAAACTTACTTGTTGAAGCCTCTCAAATTCGAGGGTCCTTGTAACCCTAAACGAGTACATTTTGAGGTTAGAGGAACTATCATGGCTCCGAGCGTGGCTGCATGGCGTAGCCATAACGAAGAAAACTGGATCCAATTCTTGGACATAGCGGGTCTCAATATCAATGGAGCAGGTCAATTCGATGGCCAGAGTTCTTCTTGGTGGAAAGATTGCCATTCAAACTGCACACCTCCAACGGCATTATTTTTCCAAAATTGCACCGGGCTCCGACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCATAAACGCTTGCAATGATGTCGTTATCTCAAACGTTCACATCATTGCTCCCGAAAACATCCCCAACACATACGGGTTCAACATTTCTAGATCAAACAATGTTCTTATTCAAAACTCCTTAATCGAAACAGGTGACGAATGTATTTCTCTATACAACGGTTCTTCTAATATCAATATCGTCGGTGTTACATGTGGACCTGGTTACGGTATAAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATCGAAAACATCAATGTAAGCAATTGCACCCTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTACAAAGGGTGGACATGGATATGTAAGAAACGTCGTATACAAAAAAATAACGCTAATTGATACCCAATTGCCGATCGTTATTGATCAACAGTATTCAACTTTTGCCAACACGAGAAAAATTAAGGAAATGGGTATAAAAGTGATGGATGTGACGTATCGTGAAATTCATGGAATATCAACAAATGAATCGACAGTCATTTTGAATTGTTCTCAAACTCGTTGTAGTAATATTGTAATGGAAGACGTAAGCATACGGACGTCATCTCCAAAAAAACAAGTCAAAGCGTTTTGCCAAAATGTTGATGGAAGAGCCGTATCTACCGTCCCAATCGTGTCTTGTTTATCGAAATCTAATTAA

Coding sequence (CDS)

ATGGAGAGCAAAAGAGTTGCTTTTGTGGCGATACTGATGATAATGGTTATGGTAGGTTTAGCCTCGGCGGCCTGCACGAACGCGATCAAGCTCGTGAGCGACGGTGAAAATGAGATGGAAAGTGGGTTTGGAAGCAAGATGATGAAAAATGAGTTTCCAAGCCCAATCGATAGATTAGAAATGATGAAGCTATTTTCTGCTTTAGTTTTTATGTTTTCCACAGTCATTTTAAGCTCGTGGATTGTATCAGCTTCATTTGATTTTAATGTTCTTGATTATGGAGCTGTTGGAAATGGAAAAACGGATGATTCACAAGCTTTTTTGAAAGCATGGAAAGACGTGTGTCGGGCAACGATAGGCATTCCAACATTGCGTGTACCAATTGGAAAAACTTACTTGTTGAAGCCTCTCAAATTCGAGGGTCCTTGTAACCCTAAACGAGTACATTTTGAGGTTAGAGGAACTATCATGGCTCCGAGCGTGGCTGCATGGCGTAGCCATAACGAAGAAAACTGGATCCAATTCTTGGACATAGCGGGTCTCAATATCAATGGAGCAGGTCAATTCGATGGCCAGAGTTCTTCTTGGTGGAAAGATTGCCATTCAAACTGCACACCTCCAACGGCATTATTTTTCCAAAATTGCACCGGGCTCCGACTACGAGAAACTAAGCACATCAATAGTGTCAAAAATCATATCTCCATAAACGCTTGCAATGATGTCGTTATCTCAAACGTTCACATCATTGCTCCCGAAAACATCCCCAACACATACGGGTTCAACATTTCTAGATCAAACAATGTTCTTATTCAAAACTCCTTAATCGAAACAGGTGACGAATGTATTTCTCTATACAACGGTTCTTCTAATATCAATATCGTCGGTGTTACATGTGGACCTGGTTACGGTATAAGCATTAGAAGCTTAGGAGAAGATGGAGGATATAACGTAATCGAAAACATCAATGTAAGCAATTGCACCCTAAAGGGCACTCAAAATGGATTGAGAATCAAGACTACAAAGGGTGGACATGGATATGTAAGAAACGTCGTATACAAAAAAATAACGCTAATTGATACCCAATTGCCGATCGTTATTGATCAACAGTATTCAACTTTTGCCAACACGAGAAAAATTAAGGAAATGGGTATAAAAGTGATGGATGTGACGTATCGTGAAATTCATGGAATATCAACAAATGAATCGACAGTCATTTTGAATTGTTCTCAAACTCGTTGTAGTAATATTGTAATGGAAGACGTAAGCATACGGACGTCATCTCCAAAAAAACAAGTCAAAGCGTTTTGCCAAAATGTTGATGGAAGAGCCGTATCTACCGTCCCAATCGTGTCTTGTTTATCGAAATCTAATTAA

Protein sequence

MESKRVAFVAILMIMVMVGLASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLEMMKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMEDVSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN

Homology

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BLAST of Carg19686 vs. NCBI nr
Match: KAG7014943.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 915.6 bits (2365), Expect = 1.7e-262
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0


Query: 1   MESKRVAFVAILMIMVMVGLASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLE 60
           MESKRVAFVAILMIMVMVGLASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLE
Sbjct: 1   MESKRVAFVAILMIMVMVGLASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLE 60

Query: 61  MMKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIG 120
           MMKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIG
Sbjct: 61  MMKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIG 120

Query: 121 IPTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAG 180
           IPTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAG
Sbjct: 121 IPTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAG 180

Query: 181 LNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACND 240
           LNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACND
Sbjct: 181 LNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACND 240

Query: 241 VVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGP 300
           VVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGP
Sbjct: 241 VVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGP 300

Query: 301 GYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDT 360
           GYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDT
Sbjct: 301 GYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDT 360

Query: 361 QLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMED 420
           QLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMED
Sbjct: 361 QLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMED 420

Query: 421 VSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 458
           VSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN
Sbjct: 421 VSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 457

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BLAST of Carg19686 vs. NCBI nr
Match: XP_023552846.1 (probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739.2 bits (1907), Expect = 2.2e-209
Identity = 376/455 (82.64%), Postives = 401/455 (88.13%), Query Frame = 0


Query: 4   KRVAFVAILMIMVMVGLASAAC-TNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLEMM 63
           K VA   I M++V    A+ A   +++ LV DG  ++  G   K+MK    +  +R+   
Sbjct: 5   KNVAVALIAMMLVATSSAAYAIDDDSMDLVFDG-RDINHGVPRKIMKWGVLNHEERV--- 64

Query: 64  KLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIP 123
                   +      S  +  +S DFNVLDYGAVGNGKTDDSQAFLKAWKDVCRA+ GIP
Sbjct: 65  ----CPKILMECKKDSDCLAESSSDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRASTGIP 124

Query: 124 TLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLN 183
           TLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLN
Sbjct: 125 TLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLN 184

Query: 184 INGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVV 243
           INGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGL+LRETKHINSVKNHIS+NACNDVV
Sbjct: 185 INGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLQLRETKHINSVKNHISLNACNDVV 244

Query: 244 ISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGY 303
           ISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGY
Sbjct: 245 ISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGY 304

Query: 304 GISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQL 363
           GISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNV+Y+KITLIDTQL
Sbjct: 305 GISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVIYEKITLIDTQL 364

Query: 364 PIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMEDVS 423
           PIVIDQQYST ANTRKIKEMGIKVMDVTYREIHGISTNE+TVILNCSQTRCSNIVMEDVS
Sbjct: 365 PIVIDQQYSTLANTRKIKEMGIKVMDVTYREIHGISTNETTVILNCSQTRCSNIVMEDVS 424

Query: 424 IRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 458
           I+ S+P K+VKAFCQNVDGRAVSTVPIV+CLSKSN
Sbjct: 425 IQMSAPGKEVKAFCQNVDGRAVSTVPIVTCLSKSN 451

[Close]

BLAST of Carg19686 vs. NCBI nr
Match: KAG6576917.1 (putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 607.8 bits (1566), Expect = 7.5e-170
Identity = 298/301 (99.00%), Postives = 300/301 (99.67%), Query Frame = 0


Query: 157 MAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCT 216
           MAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCT
Sbjct: 1   MAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCT 60

Query: 217 GLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIE 276
           GLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIE
Sbjct: 61  GLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIE 120

Query: 277 TGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLR 336
           TGDECISLYNGSSNINIVGVTCGP YGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLR
Sbjct: 121 TGDECISLYNGSSNINIVGVTCGPSYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLR 180

Query: 337 IKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHG 396
           IKTTKGGHGYVRNVVYKKITLI+TQLPIVIDQQYSTFANT+KIKEMGIKVMDVTYREIHG
Sbjct: 181 IKTTKGGHGYVRNVVYKKITLINTQLPIVIDQQYSTFANTKKIKEMGIKVMDVTYREIHG 240

Query: 397 ISTNESTVILNCSQTRCSNIVMEDVSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKS 456
           ISTNESTVILNCSQTRCSNIVMEDVSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKS
Sbjct: 241 ISTNESTVILNCSQTRCSNIVMEDVSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKS 300

Query: 457 N 458
           N
Sbjct: 301 N 301

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BLAST of Carg19686 vs. NCBI nr
Match: XP_022922566.1 (probable polygalacturonase At3g15720 [Cucurbita moschata])

HSP 1 Score: 564.7 bits (1454), Expect = 7.3e-157
Identity = 281/322 (87.27%), Postives = 289/322 (89.75%), Query Frame = 0


Query: 20  LASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLEMMKLFSALVFMFSTVILSS 79
           LASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPID                +VILS 
Sbjct: 80  LASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPID----------------SVILSL 139

Query: 80  WIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKF 139
           W VSAS DFNVLDYGAVGNGKTDDSQAFLK WKDVC ATIGIPTLRVPIGKTYLLKPLKF
Sbjct: 140 WNVSASSDFNVLDYGAVGNGKTDDSQAFLKVWKDVCGATIGIPTLRVPIGKTYLLKPLKF 199

Query: 140 EGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKD 199
           EGPCNPKRV+FEVRGTIMAPSVAAW SHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKD
Sbjct: 200 EGPCNPKRVNFEVRGTIMAPSVAAWPSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKD 259

Query: 200 CHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYG 259
           CHSNCTPPT         LRLRETKHINSVKNHISINACND +IS+VHIIAPENIPNTYG
Sbjct: 260 CHSNCTPPT---------LRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYG 319

Query: 260 FNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIE 319
           FN+SRSNNV+IQNSLIETGDECISLYNGS NINIVGVTCGPGYGISIRSLGEDGGYNVIE
Sbjct: 320 FNVSRSNNVVIQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIE 376

Query: 320 NINVSNCTLKGTQNGLRIKTTK 342
           NINVSNCTLKGTQNGLRIKT++
Sbjct: 380 NINVSNCTLKGTQNGLRIKTSQ 376

[Close]

BLAST of Carg19686 vs. NCBI nr
Match: XP_022141108.1 (probable polygalacturonase At3g15720 [Momordica charantia])

HSP 1 Score: 542.0 bits (1395), Expect = 5.1e-150
Identity = 254/396 (64.14%), Postives = 315/396 (79.55%), Query Frame = 0


Query: 62  MKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGI 121
           MKL  ALVF+FS  +LSSW+VS S +FNVLDYGA+GNG+TDDSQAFL+AW D C AT GI
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 122 PTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGL 181
           P L  P+GK Y+L PLKF+GPC  K+V F + GT++AP+  +W S + + WIQF DI GL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 182 NINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDV 241
            +NG+GQFDGQ SSWWKDC  +CT PTALF  NC GL+LRE KHINS +NHISINACND 
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 242 VISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPG 301
           +IS++HI+APE+ PNT G +IS SNNVLIQNS + TGD+CI+L NGS+NINIVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 302 YGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQ 361
           +GISI SLGE+G YNV+E+I+V NC LKGTQNG+RIKT + G+GY R + +++ITLID +
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 362 LPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMEDV 421
            PI+IDQ YS  A  +K + + IKV DVTYRE+HG S NE+ +ILNCS++RCSNI+ME+V
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

Query: 422 SIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 458
           SI+   P K+ K  CQNVDGR+ S +P V CLSK N
Sbjct: 361 SIKNIDPXKEAKXLCQNVDGRSTSAIPSVPCLSKQN 396

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BLAST of Carg19686 vs. ExPASy Swiss-Prot
Match: Q9LW07 (Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g15720 PE=3 SV=1)

HSP 1 Score: 305.1 bits (780), Expect = 1.4e-81
Identity = 169/381 (44.36%), Postives = 235/381 (61.68%), Query Frame = 0


Query: 81  IVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFE 140
           I ++S   +V  +GAVG+G TDDSQAFLKAW+ VC  T G     VP G T++L+PLKF+
Sbjct: 17  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGT-GDGQFVVPAGMTFMLQPLKFQ 76

Query: 141 GPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDC 200
           G C    V  ++ G ++APS   W+  +++ WI F DI GL I G G+ +GQ SSWW+  
Sbjct: 77  GSCKSTPVFVQMLGKLVAPSKGNWKG-DKDQWILFTDIEGLVIEGDGEINGQGSSWWEHK 136

Query: 201 HSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGF 260
            S    PTAL F++C  LRL    H++S   HI I+ CN V IS++ I APE+ PNT G 
Sbjct: 137 GSR---PTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGI 196

Query: 261 NISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIEN 320
           ++  S+NV+IQ+ +I TGD+CI++ +G+SNI+I G+ CGPG+GISI SLG+DG    +EN
Sbjct: 197 DVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVEN 256

Query: 321 INVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYS--TFANTRK 380
           + V NC  +GT NG RIKT +GG GY R + +  ITL + + PI+IDQ Y+     N + 
Sbjct: 257 VCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKD 316

Query: 381 IKEMGIKVMDVTYREIHGISTNESTVILNCSQ-TRCSNIVMEDVSIRT-SSPKKQV-KAF 440
            K   ++V  V +    G S +E  V   CS+   C+ I + D+ I T SS   QV +  
Sbjct: 317 RKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQ 376

Query: 441 CQNVDGRAVSTVPIVSCLSKS 457
           C NV G +   VP + CL  S
Sbjct: 377 CLNVRGASTIAVPGLECLELS 392

[Close]

BLAST of Carg19686 vs. ExPASy Swiss-Prot
Match: Q9SFB7 (Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2)

HSP 1 Score: 251.1 bits (640), Expect = 2.3e-65
Identity = 137/367 (37.33%), Postives = 206/367 (56.13%), Query Frame = 0


Query: 83  SASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFEGP 142
           S+   FNV  +GA  NG  DDS+AF+KAW+  C +T GI  +  P  + Y+LK + F GP
Sbjct: 66  SSPRSFNVNTFGAKANG-NDDSKAFMKAWEAACSST-GIVYIVAPKNRDYMLKAVTFSGP 125

Query: 143 CNPKRVHFEVRGTIMA---PSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWW-K 202
           C    + F++ G I A   PS    R H    WI F ++  L + G G+ DG    WW K
Sbjct: 126 CKSSLIIFKIYGRIEAWENPSDYKERRH----WIVFENVNNLRVEGGGRIDGNGHIWWPK 185

Query: 203 DCHSN-----CTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPEN 262
            C  N        PTA+ F  C  LR+   +  N+ + H++   C +V   N+ + +P +
Sbjct: 186 SCKINPQLPCLGAPTAVTFVECNNLRVSNIRLENAQQMHLTFQDCKNVKALNLMVTSPAD 245

Query: 263 IPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDG 322
            PNT G ++S + N+LIQ+S++ TGD+CIS+ +GS N+   G+TCGPG+GISI SLGED 
Sbjct: 246 SPNTDGIHVSGTQNILIQDSIVRTGDDCISIVSGSENVRATGITCGPGHGISIGSLGEDN 305

Query: 323 GYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTF 382
               + N+ V+  TL GT NG+RIKT +GGHG  +N++++ I + +   PI+I+Q Y   
Sbjct: 306 SEAYVSNVVVNKATLIGTTNGVRIKTWQGGHGMAKNIIFQDIIMKNVTNPIIINQDYCDR 365

Query: 383 ANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQT-RCSNIVMEDVSIRTSSPKKQV 440
                 ++  ++V +V Y+ I G S+    V   CS+   C  I M++V +   + +   
Sbjct: 366 VEACPEQKSAVQVSNVLYKNIQGTSSRPIAVKFVCSKNIPCRGISMQNVKLVDQTQQDVS 425

[Close]

BLAST of Carg19686 vs. ExPASy Swiss-Prot
Match: Q8RY29 (Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2)

HSP 1 Score: 248.8 bits (634), Expect = 1.2e-64
Identity = 143/363 (39.39%), Postives = 212/363 (58.40%), Query Frame = 0


Query: 89  NVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFEGPCNPKRV 148
           +V D+GA G+GKTDD+QAF+ AWK  C +  G   L VP G TYLLK ++  GPCN   +
Sbjct: 69  SVSDFGAKGDGKTDDTQAFVNAWKKACSSN-GAVNLLVPKGNTYLLKSIQLTGPCN-SIL 128

Query: 149 HFEVRGTIMAPSVAAWRSHNEE--NWIQFLDIAGLNING--AGQFDGQSSSWWKD-CHSN 208
             ++ GT+   S +  RS  ++   WI F  +  L+++G   G  DG   +WW++ C  N
Sbjct: 129 TVQIFGTL---SASQKRSDYKDISKWIMFDGVNNLSVDGGDTGVVDGNGETWWQNSCKRN 188

Query: 209 ----CT-PPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTY 268
               CT  PTAL F N   L ++  K  N+ +  ISI  C++V +SNV + AP + PNT 
Sbjct: 189 KAKPCTKAPTALTFYNSKSLIVKNLKVRNAQQIQISIEKCSNVQVSNVVVTAPADSPNTD 248

Query: 269 GFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVI 328
           G +I+ + N+ +  S+I TGD+CIS+ +GS N+ I  +TCGPG+GISI SLG+D     +
Sbjct: 249 GIHITNTQNIRVSESIIGTGDDCISIESGSQNVQINDITCGPGHGISIGSLGDDNSKAFV 308

Query: 329 ENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTFANTRK 388
             + V    L GT NG+RIKT +GG G   N++++ I + + + PI+IDQ Y   +    
Sbjct: 309 SGVTVDGAKLSGTDNGVRIKTYQGGSGTASNIIFQNIQMDNVKNPIIIDQDYCDKSKCTT 368

Query: 389 IKEMGIKVMDVTYREIHGISTNESTVILNCSQT-RCSNIVMEDVSIRTSSPKKQVKAFCQ 441
            K   ++V +V YR+I G S +E+ +  NCS+   C  IV++ V+I      K  KA C 
Sbjct: 369 EKS-AVQVKNVVYRDISGTSASENAITFNCSKNYPCQGIVLDRVNI------KGGKATCT 419

[Close]

BLAST of Carg19686 vs. ExPASy Swiss-Prot
Match: O23147 (Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1)

HSP 1 Score: 248.1 bits (632), Expect = 2.0e-64
Identity = 158/445 (35.51%), Postives = 242/445 (54.38%), Query Frame = 0


Query: 12  LMIMVMVGLASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLEMMKLFSALVFM 71
           L +++M+ L  A  +N    V DG    +  F S  +       ++  +++ L S+    
Sbjct: 12  LCVLLMLSLCKALSSN----VDDGYGHEDGSFESDSLLK-----LNNDDVLSLISS---- 71

Query: 72  FSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKT 131
                L +  VS S      ++GA G+GKTDD+QAF KAWK  C +T G+ T  VP GKT
Sbjct: 72  -DETTLEASTVSVS------NFGAKGDGKTDDTQAFKKAWKKAC-STNGVTTFLVPKGKT 131

Query: 132 YLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNING--AGQF 191
           YLLK  +F GPC   R +F++ GT+ A S       ++ +W+   D+  L+I+G   G  
Sbjct: 132 YLLKSTRFRGPCKSLR-NFQILGTLSA-STKRSDYKDKNHWLILEDVNNLSIDGGSTGII 191

Query: 192 DGQSSSWWK-----DCHSNCT-PPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVI 251
           +G   +WW+     D    CT  PTAL   N   L ++  +  N+ +  ISI  CN V +
Sbjct: 192 NGNGKTWWQNSCKIDKSKPCTKAPTALTLYNLKNLNVKNLRVKNAQQIQISIEKCNKVEV 251

Query: 252 SNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYG 311
           SNV I AP + PNT G +I+ + N+ + NS I TGD+CIS+ +G+ N+ I  +TCGPG+G
Sbjct: 252 SNVEITAPGDSPNTDGIHITNTQNIRVSNSDIGTGDDCISIEDGTQNLQIFDLTCGPGHG 311

Query: 312 ISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLP 371
           ISI SLG+D     +  INV       + NG+RIKT +GG G  +N+ ++ I + + + P
Sbjct: 312 ISIGSLGDDNSKAYVSGINVDGAKFSESDNGVRIKTYQGGSGTAKNIKFQNIRMENVKNP 371

Query: 372 IVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQT-RCSNIVMEDVS 431
           I+IDQ Y    +  + +E  ++V +V Y+ I G S  +  + LNCS+   C  IV+E+V 
Sbjct: 372 IIIDQDYCD-KDKCEDQESAVQVKNVVYKNISGTSATDVAITLNCSEKYPCQGIVLENVK 426

Query: 432 IRTSSPKKQVKAFCQNVDGRAVSTV 448
           I+  +      A C+N + +   TV
Sbjct: 432 IKGGT------ASCKNANVKNQGTV 426

[Close]

BLAST of Carg19686 vs. ExPASy Swiss-Prot
Match: Q94AJ5 (Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g80170 PE=1 SV=1)

HSP 1 Score: 245.0 bits (624), Expect = 1.7e-63
Identity = 128/374 (34.22%), Postives = 206/374 (55.08%), Query Frame = 0


Query: 89  NVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFEGPCNPKRV 148
           +V ++GA GNG TDD++AF  AWK  C + +    L VP   T LL+P+   GPC   R+
Sbjct: 54  HVGNFGAKGNGVTDDTKAFADAWKTACSSKVKTRIL-VPENYTCLLRPIDLSGPCK-ARL 113

Query: 149 HFEVRGTIMAPS-VAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWW-KDC-HSNCT 208
             ++ GTI+AP+    W   N   W+ F  ++ L + G G  +G    WW + C H++  
Sbjct: 114 TLQISGTIIAPNDPDVWEGLNRRKWLYFHGLSRLTVEGGGTVNGMGQEWWERSCKHNHSN 173

Query: 209 P----PTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFN 268
           P    PTAL F  C  +R+     I+S + HI++ +C  V IS + +IAP   PNT G +
Sbjct: 174 PCRGAPTALTFHKCKNMRVENLNVIDSQQMHIALTSCRRVTISGLKVIAPATSPNTDGIH 233

Query: 269 ISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENI 328
           IS S  ++I N+ + TGD+CIS+   S+ I+I  + CGPG+GISI SLG+   +  + +I
Sbjct: 234 ISVSRGIVIDNTTVSTGDDCISIVKNSTQISISNIICGPGHGISIGSLGKSKSWEEVRDI 293

Query: 329 NVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTFANTRKIKE 388
            V    +  T NG+RIKT +GG G V  ++++ I + +   PI+IDQ Y         + 
Sbjct: 294 TVDTAIISDTANGVRIKTWQGGSGLVSKIIFRNIKMNNVSNPIIIDQYYCDSRKPCANQT 353

Query: 389 MGIKVMDVTYREIHGISTNESTVILNCSQTR-CSNIVMEDVSIRTSSPKKQVKAFCQNVD 448
             I + ++++  + G S ++  + ++CS +  C NI+++D+ +  S+     ++FC    
Sbjct: 354 SAISIENISFVHVRGTSASKEAIKISCSDSSPCRNILLQDIDLEPSNGDGFTESFCWEAY 413

Query: 449 GRAVSTVPIVSCLS 455
           G +   V    CLS
Sbjct: 414 GSSSGQVYPPPCLS 425

[Close]

BLAST of Carg19686 vs. ExPASy TrEMBL
Match: A0A6J1E4H0 (probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC111430533 PE=3 SV=1)

HSP 1 Score: 564.7 bits (1454), Expect = 3.5e-157
Identity = 281/322 (87.27%), Postives = 289/322 (89.75%), Query Frame = 0


Query: 20  LASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPIDRLEMMKLFSALVFMFSTVILSS 79
           LASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPID                +VILS 
Sbjct: 80  LASAACTNAIKLVSDGENEMESGFGSKMMKNEFPSPID----------------SVILSL 139

Query: 80  WIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKF 139
           W VSAS DFNVLDYGAVGNGKTDDSQAFLK WKDVC ATIGIPTLRVPIGKTYLLKPLKF
Sbjct: 140 WNVSASSDFNVLDYGAVGNGKTDDSQAFLKVWKDVCGATIGIPTLRVPIGKTYLLKPLKF 199

Query: 140 EGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKD 199
           EGPCNPKRV+FEVRGTIMAPSVAAW SHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKD
Sbjct: 200 EGPCNPKRVNFEVRGTIMAPSVAAWPSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKD 259

Query: 200 CHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYG 259
           CHSNCTPPT         LRLRETKHINSVKNHISINACND +IS+VHIIAPENIPNTYG
Sbjct: 260 CHSNCTPPT---------LRLRETKHINSVKNHISINACNDAIISDVHIIAPENIPNTYG 319

Query: 260 FNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIE 319
           FN+SRSNNV+IQNSLIETGDECISLYNGS NINIVGVTCGPGYGISIRSLGEDGGYNVIE
Sbjct: 320 FNVSRSNNVVIQNSLIETGDECISLYNGSFNINIVGVTCGPGYGISIRSLGEDGGYNVIE 376

Query: 320 NINVSNCTLKGTQNGLRIKTTK 342
           NINVSNCTLKGTQNGLRIKT++
Sbjct: 380 NINVSNCTLKGTQNGLRIKTSQ 376

[Close]

BLAST of Carg19686 vs. ExPASy TrEMBL
Match: A0A6J1CJJ6 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011582 PE=3 SV=1)

HSP 1 Score: 542.0 bits (1395), Expect = 2.5e-150
Identity = 254/396 (64.14%), Postives = 315/396 (79.55%), Query Frame = 0


Query: 62  MKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGI 121
           MKL  ALVF+FS  +LSSW+VS S +FNVLDYGA+GNG+TDDSQAFL+AW D C AT GI
Sbjct: 1   MKLHLALVFIFSIHLLSSWVVSTSPNFNVLDYGAIGNGQTDDSQAFLRAWXDACGATEGI 60

Query: 122 PTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGL 181
           P L  P+GK Y+L PLKF+GPC  K+V F + GT++AP+  +W S + + WIQF DI GL
Sbjct: 61  PALHAPVGKIYMLNPLKFQGPCKSKQVIFHLGGTVVAPTKDSWPSDDRDRWIQFSDIDGL 120

Query: 182 NINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDV 241
            +NG+GQFDGQ SSWWKDC  +CT PTALF  NC GL+LRE KHINS +NHISINACND 
Sbjct: 121 MVNGSGQFDGQGSSWWKDCDKHCTRPTALFLHNCNGLQLREMKHINSGRNHISINACNDA 180

Query: 242 VISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPG 301
           +IS++HI+APE+ PNT G +IS SNNVLIQNS + TGD+CI+L NGS+NINIVGVTCGPG
Sbjct: 181 IISDLHIMAPEDSPNTDGIDISGSNNVLIQNSFMGTGDDCIALNNGSTNINIVGVTCGPG 240

Query: 302 YGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQ 361
           +GISI SLGE+G YNV+E+I+V NC LKGTQNG+RIKT + G+GY R + +++ITLID +
Sbjct: 241 HGISIGSLGENGQYNVVEDIHVKNCLLKGTQNGVRIKTWQNGYGYARGITFEQITLIDVK 300

Query: 362 LPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMEDV 421
            PI+IDQ YS  A  +K + + IKV DVTYRE+HG S NE+ +ILNCS++RCSNI+ME+V
Sbjct: 301 NPIIIDQYYSDLAKAQKNRLLPIKVSDVTYREVHGTSANENAIILNCSRSRCSNIIMENV 360

Query: 422 SIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 458
           SI+   P K+ K  CQNVDGR+ S +P V CLSK N
Sbjct: 361 SIKNIDPXKEAKXLCQNVDGRSTSAIPSVPCLSKQN 396

[Close]

BLAST of Carg19686 vs. ExPASy TrEMBL
Match: A0A6J1CIX8 (probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011581 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 1.3e-143
Identity = 251/393 (63.87%), Postives = 309/393 (78.63%), Query Frame = 0


Query: 62  MKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGI 121
           MKL   L+F+FS  ILSSWIVSAS  FNVLDYGA+GNG+ DDSQAFL+AW DVC AT GI
Sbjct: 1   MKLHFVLLFIFSINILSSWIVSASPSFNVLDYGAIGNGQADDSQAFLRAWNDVCGATEGI 60

Query: 122 PTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGL 181
           PTL  P GK Y+LKPLKF+GPC  K+V F ++GT++AP   +W  ++ E WIQF DI GL
Sbjct: 61  PTLHAPHGKIYMLKPLKFQGPCKSKQVTFHLKGTLVAPKKGSWPKYDRERWIQFSDIDGL 120

Query: 182 NINGAGQFDGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDV 241
            +NG GQFDGQ SSWW+D   +C  PTALF  NC GL+LRE KHINS KNHISINACNDV
Sbjct: 121 IVNGTGQFDGQGSSWWED-WEHCQRPTALFLHNCNGLQLREMKHINSAKNHISINACNDV 180

Query: 242 VISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPG 301
           +IS++HIIAPE+ PNT G ++SRSNNVLIQNS + TGD+CI+L NGSSNI+IVGVTCGPG
Sbjct: 181 IISDLHIIAPEDSPNTDGIDVSRSNNVLIQNSFMGTGDDCIALNNGSSNIDIVGVTCGPG 240

Query: 302 YGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQ 361
           +GISI SLG +  YNV+ENI+V NC LKGTQNG+RIKT +GG+GY RN+ +++ITL+  +
Sbjct: 241 HGISIGSLGGNREYNVVENIHVKNCLLKGTQNGMRIKTWQGGYGYARNITFEQITLMGAK 300

Query: 362 LPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMEDV 421
            PI+IDQ Y+ F +    +   IKV +VTYRE++G S NE+ +ILNCS+ RCSNI++E+V
Sbjct: 301 NPIIIDQYYTDFFSI-FFQLFDIKVSNVTYREVNGTSANENAIILNCSRNRCSNIILENV 360

Query: 422 SIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLS 455
           SI+   PK + KAFCQNVDGR+   VP V CLS
Sbjct: 361 SIKPIDPKMEAKAFCQNVDGRSTYAVPTVPCLS 391

[Close]

BLAST of Carg19686 vs. ExPASy TrEMBL
Match: A0A0A0KZ95 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 2.1e-141
Identity = 240/397 (60.45%), Postives = 308/397 (77.58%), Query Frame = 0


Query: 62  MKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGI 121
           MKL SA +F++S  ILSSW V  S DFNVLDYGA+GNGKTDDS+AFLKAW DVC AT   
Sbjct: 1   MKLLSAAIFLYSIAILSSWTVLGSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATEES 60

Query: 122 PTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGL 181
           PTL VP GKTYLL PLKF+GPC  K    ++ GT+MAP+   W S  +  WIQF DI GL
Sbjct: 61  PTLHVPDGKTYLLNPLKFQGPCKSK----QLGGTLMAPTKDEWISDGKGQWIQFFDIEGL 120

Query: 182 NINGAGQFDGQSSSWWKDC-HSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACND 241
           N+ G G+FDGQ S WWKDC H +C  PTALFF NC GL+++  KHINS KNHISIN C+D
Sbjct: 121 NLQGGGRFDGQGSLWWKDCDHKHCKRPTALFFHNCNGLQMKGMKHINSAKNHISINMCHD 180

Query: 242 VVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGP 301
           V+ S++HI APE+ PNT G +IS S N+ I++S + TGD+CI++ NGSSNINI G+TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISESTNIFIEDSFMATGDDCIAINNGSSNININGITCGP 240

Query: 302 GYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDT 361
           G+GISI SLG+DG YNV+EN++VSNC L+ TQNG+RIKT +GG+GY +N+ ++KIT+ + 
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRSTQNGIRIKTWEGGYGYAKNITFEKITMKNA 300

Query: 362 QLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMED 421
           + PI+IDQ YS++A +RK+K M IKV DVTYRE++G S NE  +  NCSQ RCSNI++++
Sbjct: 301 KNPIIIDQYYSSYAYSRKMKGMDIKVSDVTYREVNGTSANEDAITFNCSQARCSNIILDN 360

Query: 422 VSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 458
           V+I+ ++P ++ KA CQN DG+ +S VP VSCLSKS+
Sbjct: 361 VNIKMTNPGEKAKAVCQNADGKVLSAVPPVSCLSKSH 393

[Close]

BLAST of Carg19686 vs. ExPASy TrEMBL
Match: A0A5D3CYS8 (Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold130G00190 PE=3 SV=1)

HSP 1 Score: 509.2 bits (1310), Expect = 1.8e-140
Identity = 236/397 (59.45%), Postives = 307/397 (77.33%), Query Frame = 0


Query: 62  MKLFSALVFMFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGI 121
           MKL  A +  +S  ILSS  V +S DFNVLDYGA+GNGKTDDS+AFLKAW DVC AT   
Sbjct: 1   MKLLLAAILTYSIAILSSRTVLSSSDFNVLDYGAIGNGKTDDSKAFLKAWNDVCGATQDS 60

Query: 122 PTLRVPIGKTYLLKPLKFEGPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGL 181
           PTL VP GKTYLL PLKF+GPC   +V F++ GT+MAP+   W +  +  W+QF+D+ GL
Sbjct: 61  PTLHVPDGKTYLLSPLKFQGPCKSNQVIFDLGGTLMAPTKDEWPNDGKSKWVQFVDVEGL 120

Query: 182 NINGAGQFDGQSSSWWKDC-HSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACND 241
           N+ G G+FDGQ S WWK C H  C  PTALFF NC GL++   KH+NS KNHISIN CND
Sbjct: 121 NVQGGGRFDGQGSCWWKGCDHKQCKRPTALFFHNCNGLQMNGMKHVNSAKNHISINLCND 180

Query: 242 VVISNVHIIAPENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGP 301
           V+ S++HI APE+ PNT G +IS+S N+ IQ+S I TGD+C+++ NGSSNINI  +TCGP
Sbjct: 181 VIFSHLHISAPEDSPNTDGIDISQSTNIFIQDSFIGTGDDCVAINNGSSNININTITCGP 240

Query: 302 GYGISIRSLGEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDT 361
           G+GISI SLG+DG YNV+EN++VSNC L+ TQNG+RIKT +GG+GY +N+ ++KITL ++
Sbjct: 241 GHGISIGSLGKDGEYNVVENVHVSNCLLRNTQNGIRIKTWEGGYGYAKNITFEKITLKNS 300

Query: 362 QLPIVIDQQYSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQTRCSNIVMED 421
           + PI+IDQ Y+++A +RKIK M IKV DVTYRE++G S NE  +  NCSQ RCSNI++++
Sbjct: 301 KNPIIIDQYYTSYAYSRKIKGMDIKVSDVTYREVNGTSANEEAITFNCSQARCSNIILDN 360

Query: 422 VSIRTSSPKKQVKAFCQNVDGRAVSTVPIVSCLSKSN 458
           V+I+ ++P ++ KA CQNVDG+ +S VP VSCLSKS+
Sbjct: 361 VNIKMTNPGEEAKAVCQNVDGKVLSAVPPVSCLSKSH 397

[Close]

BLAST of Carg19686 vs. TAIR 10
Match: AT4G35670.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 306.6 bits (784), Expect = 3.3e-83
Identity = 158/372 (42.47%), Postives = 228/372 (61.29%), Query Frame = 0


Query: 87  DFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFEGPCNPK 146
           ++NVL++ A G+G+TDDS+AFL+AW   C     I TL +P GKT+LL+P  F+GPC   
Sbjct: 23  NYNVLNFDAKGDGQTDDSEAFLQAWTAACGGDGDIKTLLIPSGKTFLLQPTVFQGPCKSS 82

Query: 147 RVHFEVRGTIMAPS-VAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDCHSNCT 206
            +  ++ GTI+APS   AW       WI+F  ++GL I G+G  D + SS+W+       
Sbjct: 83  SIKVQLDGTIVAPSDKFAWSDPISRMWIKFSTVSGLIIVGSGTIDSRGSSFWELNLKASQ 142

Query: 207 PPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRS 266
            PTAL    C  LR+     I+S KNHISI  CN V ISN+++ APE  PNT G +IS S
Sbjct: 143 RPTALHISKCDNLRINGITSIDSPKNHISIKTCNTVAISNINLFAPETSPNTDGIDISDS 202

Query: 267 NNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSN 326
            N+ I +S I+TGD+CI++ +GSSNINI G+ CGPG+GIS+ SLG  G    + ++ V++
Sbjct: 203 TNINIFDSTIQTGDDCIAINSGSSNINITGINCGPGHGISVGSLGAGGAEAKVSDVQVTH 262

Query: 327 CTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTFANTRKIKEMGIK 386
           CT   T NG RIKT  GG GY RN+ +  ITL++T+ PI+IDQ Y      R  +E  + 
Sbjct: 263 CTFNQTTNGARIKTWLGGQGYARNISFTDITLVNTKNPIIIDQHY--IDKGRLTEESAVA 322

Query: 387 VMDVTYREIHGISTNESTVILNCSQ-TRCSNIVMEDVSIRTSSPKKQVKAFCQNVDGRAV 446
           + +V + +  G S+N++ + L CS+ T C ++VM+ + I  ++  K  K  CQ VDG + 
Sbjct: 323 ISNVKFVDFRGTSSNKNAITLKCSETTHCVDVVMDGIDITMANGGKP-KVNCQYVDGESS 382

Query: 447 STVPIVSCLSKS 457
            T  +  C   +
Sbjct: 383 DTDLMRDCFKNN 391

[Close]

BLAST of Carg19686 vs. TAIR 10
Match: AT3G15720.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 9.7e-83
Identity = 169/381 (44.36%), Postives = 235/381 (61.68%), Query Frame = 0


Query: 81  IVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFE 140
           I ++S   +V  +GAVG+G TDDSQAFLKAW+ VC  T G     VP G T++L+PLKF+
Sbjct: 17  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGT-GDGQFVVPAGMTFMLQPLKFQ 76

Query: 141 GPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDC 200
           G C    V  ++ G ++APS   W+  +++ WI F DI GL I G G+ +GQ SSWW+  
Sbjct: 77  GSCKSTPVFVQMLGKLVAPSKGNWKG-DKDQWILFTDIEGLVIEGDGEINGQGSSWWEHK 136

Query: 201 HSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGF 260
            S    PTAL F++C  LRL    H++S   HI I+ CN V IS++ I APE+ PNT G 
Sbjct: 137 GSR---PTALKFRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGI 196

Query: 261 NISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIEN 320
           ++  S+NV+IQ+ +I TGD+CI++ +G+SNI+I G+ CGPG+GISI SLG+DG    +EN
Sbjct: 197 DVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVEN 256

Query: 321 INVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYS--TFANTRK 380
           + V NC  +GT NG RIKT +GG GY R + +  ITL + + PI+IDQ Y+     N + 
Sbjct: 257 VCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKD 316

Query: 381 IKEMGIKVMDVTYREIHGISTNESTVILNCSQ-TRCSNIVMEDVSIRT-SSPKKQV-KAF 440
            K   ++V  V +    G S +E  V   CS+   C+ I + D+ I T SS   QV +  
Sbjct: 317 RKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQ 376

Query: 441 CQNVDGRAVSTVPIVSCLSKS 457
           C NV G +   VP + CL  S
Sbjct: 377 CLNVRGASTIAVPGLECLELS 392

[Close]

BLAST of Carg19686 vs. TAIR 10
Match: AT5G27530.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 305.1 bits (780), Expect = 9.7e-83
Identity = 155/372 (41.67%), Postives = 228/372 (61.29%), Query Frame = 0


Query: 88  FNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFEGPCNPKR 147
           +NVL +GA G+G+TDDS AFL+AW   C     I TL +P GKTYLL+P++F+GPC    
Sbjct: 53  YNVLKFGAEGDGQTDDSNAFLQAWNATCGGEENINTLFIPSGKTYLLQPIEFKGPCKSTS 112

Query: 148 VHFEVRGTIMAPS-VAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDCHSNCTP 207
           +  ++ G I+APS + +W +   + WI F  + GL I+G+G  +G+ SS+W+        
Sbjct: 113 IKLQLDGIIVAPSNITSWSNPKSQTWISFSGVPGLMIDGSGTINGRGSSFWE-------- 172

Query: 208 PTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGFNISRSN 267
             AL   NC  L +     I+S K+HISI  C+ V IS ++I+APEN PNT G +IS S 
Sbjct: 173 --ALHICNCNNLTINGITSIDSPKSHISIKNCHYVAISKINILAPENSPNTDGIDISYST 232

Query: 268 NVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIENINVSNC 327
           NV I +S I+TGD+CI++  GSS+INI  V CGPG+GIS+ SLG DG    + ++ V+ C
Sbjct: 233 NVNIFDSTIQTGDDCIAINTGSSSINITQVNCGPGHGISVGSLGADGENAAVSDVYVTQC 292

Query: 328 TLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYSTFANTRKIKEMGIKV 387
           T   T NG RIKT +GG GY RN+ ++ ITLI+ Q PI+IDQQY+        K+  + +
Sbjct: 293 TFNKTTNGARIKTWQGGQGYARNISFENITLINVQNPIIIDQQYTDKVLLDATKDSAVAI 352

Query: 388 MDVTYREIHGISTNESTVILNCSQ-TRCSNIVMEDVSIRTSSPKKQVKAFCQNVDGRAVS 447
             V Y    G + NE  ++L CS  T C ++V++D+ +   + +K  K  C+NV+G +  
Sbjct: 353 SSVKYVGFQGTTLNEDAIMLKCSAITYCKDMVIDDIEVTMENGEKP-KVECENVEGESSD 412

Query: 448 TVPIVSCLSKSN 458
              +  C + ++
Sbjct: 413 NDLMRECFNSNS 413

[Close]

BLAST of Carg19686 vs. TAIR 10
Match: AT3G15720.2 (Pectin lyase-like superfamily protein )

HSP 1 Score: 298.1 bits (762), Expect = 1.2e-80
Identity = 166/381 (43.57%), Postives = 232/381 (60.89%), Query Frame = 0


Query: 81  IVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGKTYLLKPLKFE 140
           I ++S   +V  +GAVG+G TDDSQAFLKAW+ VC  T G     VP G T++L+PLKF+
Sbjct: 16  IFTSSNALDVTQFGAVGDGVTDDSQAFLKAWEAVCSGT-GDGQFVVPAGMTFMLQPLKFQ 75

Query: 141 GPCNPKRVHFEVRGTIMAPSVAAWRSHNEENWIQFLDIAGLNINGAGQFDGQSSSWWKDC 200
           G C    V  ++ G ++APS   W+  +++ WI F DI GL I G G+ +GQ SSWW+  
Sbjct: 76  GSCKSTPVFVQMLGKLVAPSKGNWKG-DKDQWILFTDIEGLVIEGDGEINGQGSSWWE-- 135

Query: 201 HSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHIIAPENIPNTYGF 260
           H    P     F++C  LRL    H++S   HI I+ CN V IS++ I APE+ PNT G 
Sbjct: 136 HKGSRPT----FRSCNNLRLSGLTHLDSPMAHIHISECNYVTISSLRINAPESSPNTDGI 195

Query: 261 NISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSLGEDGGYNVIEN 320
           ++  S+NV+IQ+ +I TGD+CI++ +G+SNI+I G+ CGPG+GISI SLG+DG    +EN
Sbjct: 196 DVGASSNVVIQDCIIATGDDCIAINSGTSNIHISGIDCGPGHGISIGSLGKDGETATVEN 255

Query: 321 INVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQYS--TFANTRK 380
           + V NC  +GT NG RIKT +GG GY R + +  ITL + + PI+IDQ Y+     N + 
Sbjct: 256 VCVQNCNFRGTMNGARIKTWQGGSGYARMITFNGITLDNVENPIIIDQFYNGGDSDNAKD 315

Query: 381 IKEMGIKVMDVTYREIHGISTNESTVILNCSQ-TRCSNIVMEDVSIRT-SSPKKQV-KAF 440
            K   ++V  V +    G S +E  V   CS+   C+ I + D+ I T SS   QV +  
Sbjct: 316 RKSSAVEVSKVVFSNFIGTSKSEYGVDFRCSERVPCTEIFLRDMKIETASSGSGQVAQGQ 375

Query: 441 CQNVDGRAVSTVPIVSCLSKS 457
           C NV G +   VP + CL  S
Sbjct: 376 CLNVRGASTIAVPGLECLELS 388

[Close]

BLAST of Carg19686 vs. TAIR 10
Match: AT5G44840.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 290.0 bits (741), Expect = 3.2e-78
Identity = 149/384 (38.80%), Postives = 231/384 (60.16%), Query Frame = 0


Query: 71  MFSTVILSSWIVSASFDFNVLDYGAVGNGKTDDSQAFLKAWKDVCRATIGIPTLRVPIGK 130
           +F   +L S +V+    ++VL++GA GNG TDDS+AF+KAW  +C +     T  +P  K
Sbjct: 8   VFLLTLLHSILVNGQI-YDVLEFGADGNGITDDSKAFVKAWSAMCGSGGNSKTFIIPSNK 67

Query: 131 TYLLKPLKFEGPCNPKRVHFEVRGTIMAP-SVAAWRSHNEENWIQFLDIAGLNINGAGQF 190
           T+LL+PL F+GPC    V  +  G I+AP + AAW  +    W+ F +I GL +NG+G  
Sbjct: 68  TFLLQPLTFQGPCKSPSVQVKFDGKIVAPINKAAWSDYKLFRWVSFKEIIGLTVNGSGTI 127

Query: 191 DGQSSSWWKDCHSNCTPPTALFFQNCTGLRLRETKHINSVKNHISINACNDVVISNVHII 250
            G+ SS+WK  H          FQ C  L +      NS KNHISI+ C  V I+ + ++
Sbjct: 128 HGRGSSFWKQLH----------FQRCNDLMITGITSFNSPKNHISISECKRVKITKIKLV 187

Query: 251 APENIPNTYGFNISRSNNVLIQNSLIETGDECISLYNGSSNINIVGVTCGPGYGISIRSL 310
           AP + PNT G NIS S++V I +++I TGD+C+++ +GS NINI  + CGPG+GIS+ S+
Sbjct: 188 APHDSPNTDGINISESSDVDIYDTVIGTGDDCVAINSGSMNINIARMNCGPGHGISVGSV 247

Query: 311 GEDGGYNVIENINVSNCTLKGTQNGLRIKTTKGGHGYVRNVVYKKITLIDTQLPIVIDQQ 370
           G DG  +++EN+ V+NCT   T NG RIKT   G GY +N+++K +T  +T+ PI+IDQ 
Sbjct: 248 GRDGEESIVENVQVTNCTFIRTDNGARIKTWPNGKGYAKNILFKSLTFRETKNPIIIDQN 307

Query: 371 YSTFANTRKIKEMGIKVMDVTYREIHGISTNESTVILNCSQ-TRCSNIVMEDVSIRTSSP 430
           Y        ++E  + + +VT+ ++ G S  +  + ++CS+ T C +IV++ + I T   
Sbjct: 308 Y-VDKGRLDVEESAVAISNVTFTDLRGTSKLDEIIKIDCSKVTYCKDIVLDKIDIATVDG 367

Query: 431 KKQVKAFCQNVDGRAVSTVPIVSC 453
            K +   C NV G++++      C
Sbjct: 368 NKPI-VECSNVYGKSINANEANGC 378

The following BLAST results are available for this feature:

BLAST of Carg19686 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7014943.1	1.7e-262	100.00	putative polygalacturonase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_023552846.1	2.2e-209	82.64	probable polygalacturonase At3g15720 [Cucurbita pepo subsp. pepo]	[more]
KAG6576917.1	7.5e-170	99.00	putative polygalacturonase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022922566.1	7.3e-157	87.27	probable polygalacturonase At3g15720 [Cucurbita moschata]	[more]
XP_022141108.1	5.1e-150	64.14	probable polygalacturonase At3g15720 [Momordica charantia]	[more]

BLAST of Carg19686 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9LW07	1.4e-81	44.36	Probable polygalacturonase At3g15720 OS=Arabidopsis thaliana OX=3702 GN=At3g1572...	[more]
Q9SFB7	2.3e-65	37.33	Polygalacturonase QRT2 OS=Arabidopsis thaliana OX=3702 GN=QRT2 PE=1 SV=2	[more]
Q8RY29	1.2e-64	39.39	Polygalacturonase ADPG2 OS=Arabidopsis thaliana OX=3702 GN=ADPG2 PE=2 SV=2	[more]
O23147	2.0e-64	35.51	Polygalacturonase ADPG1 OS=Arabidopsis thaliana OX=3702 GN=ADPG1 PE=2 SV=1	[more]
Q94AJ5	1.7e-63	34.22	Probable polygalacturonase At1g80170 OS=Arabidopsis thaliana OX=3702 GN=At1g8017...	[more]

BLAST of Carg19686 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E4H0	3.5e-157	87.27	probable polygalacturonase At3g15720 OS=Cucurbita moschata OX=3662 GN=LOC1114305...	[more]
A0A6J1CJJ6	2.5e-150	64.14	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A6J1CIX8	1.3e-143	63.87	probable polygalacturonase At3g15720 OS=Momordica charantia OX=3673 GN=LOC111011...	[more]
A0A0A0KZ95	2.1e-141	60.45	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_4G025750 PE=3 SV=1	[more]
A0A5D3CYS8	1.8e-140	59.45	Putative polygalacturonase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff...	[more]

BLAST of Carg19686 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G35670.1	3.3e-83	42.47	Pectin lyase-like superfamily protein	[more]
AT3G15720.1	9.7e-83	44.36	Pectin lyase-like superfamily protein	[more]
AT5G27530.1	9.7e-83	41.67	Pectin lyase-like superfamily protein	[more]
AT3G15720.2	1.2e-80	43.57	Pectin lyase-like superfamily protein	[more]
AT5G44840.1	3.2e-78	38.80	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006626	Parallel beta-helix repeat	SMART	SM00710	pbh1	coord: 238..264 e-value: 390.0 score: 9.8 coord: 265..286 e-value: 380.0 score: 9.8 coord: 318..339 e-value: 39.0 score: 13.1 coord: 288..308 e-value: 6000.0 score: 1.7 coord: 347..368 e-value: 6900.0 score: 1.2
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 82..455 e-value: 9.9E-103 score: 346.2
IPR000743	Glycoside hydrolase, family 28	PFAM	PF00295	Glyco_hydro_28	coord: 120..440 e-value: 8.6E-76 score: 255.1
None	No IPR available	PANTHER	PTHR31375:SF232	SUBFAMILY NOT NAMED	coord: 71..453
None	No IPR available	PANTHER	PTHR31375	FAMILY NOT NAMED	coord: 71..453
None	No IPR available	PROSITE	PS51257	PROKAR_LIPOPROTEIN	coord: 1..25 score: 6.0
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 74..453

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19686-RA	Carg19686-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0005975	carbohydrate metabolic process
biological_process	GO:0010951	negative regulation of endopeptidase activity
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0004650	polygalacturonase activity
molecular_function	GO:0004867	serine-type endopeptidase inhibitor activity