Carg19649 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19649
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionPectin lyase-like superfamily protein
LocationCarg_Chr14: 11956320 .. 11958996 (-)
RNA-Seq ExpressionCarg19649
SyntenyCarg19649
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGCTTTCACCATTTTTGTTCTCTTTTCTTCTGGCCCTTTCTGTATCTGCAAGCACTTTCTCGCCGGACACGCCTCTTGAATCTGCCGACGGTGGATTTGATTCTCTTGCGCTGGAGCCCTCCGGCGAATTTCCTTTGAGTGGACGGGGTGTTTTTAATGTAAATGATTATGGGGCTGTTGCTGATGGGAAGACGGAGAACAGCTTGGTAAATTACTTTATTATTATTATTATTATTATTATTATTATAAATTTTCACTAATTTATCTACCAAATTACACCTTTAAATTTTATTAATTTTTAAAAAATTAAAATTTTTATTTTTATTTTTTAATTGATAATTTTTAGATTGACCGACCCACTCAAACTTTAAAAATTTAAATATTTTATTAATATTAGTGATGTGTTGTGACTGATAAATATTTGATATTTGAAAATAAATTACCATTTTTTTAAAATAATTAAAAAAAATAATCCATAATATAAAAGTGAGGTTTAAATTGTGAACTCTAGTCGGGTAGCTCGGTTAACCAAGTCAACCCATTGTAGGCGTTCATGGCGGCGTGGGAAGAAGCTTGTGCTTTCAAAGGAGACTCCATTTTCTTAATACCGGAGGGCACTTTCTTGGTCGCCGGAGTCACATTTTCCGGCCCATGTTTCAATAACCTCTCACCCAAAGTCCTCATTTTCGCCACTTTGATTGCTCCCACCAAACTCACCACCGACGTTTGGATCCACTTCCACTCCCTCCGCCACCTCCTCCTCACCGGCCTCGACGGATCCGCCGTTCTCAACGGCCAAGGCGCCCAAACCTGGTCCTTTGGCTCCGCCTGCCGCCGCCGCATGACTTGCCCCATTTTCACCACGGTAAACAGCATCGACTAATTTAGAAAATAATAATGGGTTTATAAACAAATAATACTTTGTCCATTGGTACGAGAACTTTTACCTGAGCCTAAAGTGGACAATATCATACCATTGTGGAGAGTCATCTTATTTTAAACTTTAATGTTTTTTTTTTTTTGGTTCAAAACAGTCTTTAAAGCTAACGGATGTATCACATGCGATAGTGAAAGACATAAGCCTAGTAGATAGCAAGGGCTTTCACGTAGCCATGCATGGGTGCGACCATATTCATCTTTATGGGTTTAATATTACGGCGCCTTGGAATAGTCAGAACACCGATGGCATCCATATTAGTAACTCCACCAATATCAACGTTGCCAATTCCCACATCGGAGTTGGTGACGATTGTGTTTCCATTGGACCGGGTAGCTTCGATATATTAGTTTCGAATATTAGATGTGGCCCAGGTCATGGAATCAGGTATTGTTTAATCCTTTCGAAGTTAATTATTTTAAAGAATTAGGTTGTGAAGCTTACAATGTAATGCAGCGTTGGGAGCCTAGGGAGGTACAAGAACGAGATGGATGTGATAGGAATAAGAGTGGAGAATTGTACCATAAGTGATACTCAAAATGGAGTGAGGGTAAAGACATGGCCGGGAAATTATTCGAGTAATGCTGCTCATTTGACGTTTCGAAACATTGTGATGAACAATGTCTCAAATCCCATTATCATAGATCAACGTTATTGTCCTAACCATTCTTGTGACATGAAAGAGGTAAACTATTTTGATACCCAGTACGATCGATCACAAGACTGTTGCTAATGATATTATAGTTAGATATCGGGCGGTGTGTCAGCGAGAATGCTGGCCCCAAGGAGGATGGATTGCGAGATCCCACATTAGTGGAAGAGGGGAACGAAACATTCCTTATAAGGGTGTGAAAACCTCTCCCTACCAAACCGTTTTAAAACTGTGAGGTCAAAGTAGACAATATTTGTTACTAATGGTATCAGAGCTAGACAACGAGCGATGTGCCAGTGAGGACACTGAACCCAAGGAGGGTGGATTGTGAGATCCCACATTGGTTGGAGAAGGGAACAAAACATTCATTATACGGGTGTGGAAACCTCTCCCTAACATTCATTACTAATTGTATAAAAGCCAGACATCGGGCGGTGTGCAGTGAGGACACTAGGCATTTGCTAGCGATGGGCTTGGGCTGTTACTAATAGTATCAGAGCTAGGCATCGGGGCAGTGTGCCAGCGAGAACACTAAGCCCAAGGAGGGTGGATTGCAAGATCCCACATTGGTTAGAGAGGGAAACGAAACATTCGTGGAAACCTCACCTCTCCCTAGCTAGCAGACGCGTTTTAAAACTGAGACCAAAGCAAACAATATCGACTAGCAGTGGGCTTGACCATTGCCAGACATCGAACGGTGTGCTAGCGAGGACACTAGGCCCAAGGAGGGTGGATTACGAGATCCCACATGCAGACACATGTTAAAACTATGAGATTAACAGCAATACGTAACGAGAGTCGATAGCTCTCATTTTTTGTTGCTTCGAGTAACGAGTGTGGTGGTTTTTTGTTTCATCAGTCATCAAGTGTGAAGCTGAGTGAGATGAACATGAGAAACATCAGTGGAACTTACAACACAAAGTTGGCAGTAAACATAGACTGCAGTTCAACCATGCCTTGTAATAACATTCACCTTTCATACATCAATCTCACCGCCACTCACCCACCGGAGAAGTATGAACTTGGGCGCTTCAATTGCATGGGTGCTCTCAATACCTTCACAGTTCTTAATTCAACATTTTAG

mRNA sequence

ATGAAGCTTTCACCATTTTTGTTCTCTTTTCTTCTGGCCCTTTCTGTATCTGCAAGCACTTTCTCGCCGGACACGCCTCTTGAATCTGCCGACGGTGGATTTGATTCTCTTGCGCTGGAGCCCTCCGGCGAATTTCCTTTGAGTGGACGGGGTGTTTTTAATGTAAATGATTATGGGGCTGTTGCTGATGGGAAGACGGAGAACAGCTTGGCGTTCATGGCGGCGTGGGAAGAAGCTTGTGCTTTCAAAGGAGACTCCATTTTCTTAATACCGGAGGGCACTTTCTTGGTCGCCGGAGTCACATTTTCCGGCCCATGTTTCAATAACCTCTCACCCAAAGTCCTCATTTTCGCCACTTTGATTGCTCCCACCAAACTCACCACCGACGTTTGGATCCACTTCCACTCCCTCCGCCACCTCCTCCTCACCGGCCTCGACGGATCCGCCGTTCTCAACGGCCAAGGCGCCCAAACCTGGTCCTTTGGCTCCGCCTGCCGCCGCCGCATGACTTGCCCCATTTTCACCACGTCTTTAAAGCTAACGGATGTATCACATGCGATAGTGAAAGACATAAGCCTAGTAGATAGCAAGGGCTTTCACGTAGCCATGCATGGGTGCGACCATATTCATCTTTATGGGTTTAATATTACGGCGCCTTGGAATAGTCAGAACACCGATGGCATCCATATTAGTAACTCCACCAATATCAACGTTGCCAATTCCCACATCGGAGTTGGTGACGATTGTGTTTCCATTGGACCGGGTAGCTTCGATATATTAGTTTCGAATATTAGATGTGGCCCAGGTCATGGAATCAGCGTTGGGAGCCTAGGGAGGTACAAGAACGAGATGGATGTGATAGGAATAAGAGTGGAGAATTGTACCATAAGTGATACTCAAAATGGAGTGAGGGTAAAGACATGGCCGGGAAATTATTCGAGTAATGCTGCTCATTTGACGTTTCGAAACATTGTGATGAACAATGTCTCAAATCCCATTATCATAGATCAACGTTATTGTCCTAACCATTCTTGTGACATGAAAGAGTTAGATATCGGGCGGTGTGTCAGCGAGAATGCTGGCCCCAAGGAGGATGGATTGCGAGATCCCACATTAGTGGAAGAGGGGAACGAAACATTCCTTATAAGGTCATCAAGTGTGAAGCTGAGTGAGATGAACATGAGAAACATCAGTGGAACTTACAACACAAAGTTGGCAGTAAACATAGACTGCAGTTCAACCATGCCTTGTAATAACATTCACCTTTCATACATCAATCTCACCGCCACTCACCCACCGGAGAAGTATGAACTTGGGCGCTTCAATTGCATGGGTGCTCTCAATACCTTCACAGTTCTTAATTCAACATTTTAG

Coding sequence (CDS)

ATGAAGCTTTCACCATTTTTGTTCTCTTTTCTTCTGGCCCTTTCTGTATCTGCAAGCACTTTCTCGCCGGACACGCCTCTTGAATCTGCCGACGGTGGATTTGATTCTCTTGCGCTGGAGCCCTCCGGCGAATTTCCTTTGAGTGGACGGGGTGTTTTTAATGTAAATGATTATGGGGCTGTTGCTGATGGGAAGACGGAGAACAGCTTGGCGTTCATGGCGGCGTGGGAAGAAGCTTGTGCTTTCAAAGGAGACTCCATTTTCTTAATACCGGAGGGCACTTTCTTGGTCGCCGGAGTCACATTTTCCGGCCCATGTTTCAATAACCTCTCACCCAAAGTCCTCATTTTCGCCACTTTGATTGCTCCCACCAAACTCACCACCGACGTTTGGATCCACTTCCACTCCCTCCGCCACCTCCTCCTCACCGGCCTCGACGGATCCGCCGTTCTCAACGGCCAAGGCGCCCAAACCTGGTCCTTTGGCTCCGCCTGCCGCCGCCGCATGACTTGCCCCATTTTCACCACGTCTTTAAAGCTAACGGATGTATCACATGCGATAGTGAAAGACATAAGCCTAGTAGATAGCAAGGGCTTTCACGTAGCCATGCATGGGTGCGACCATATTCATCTTTATGGGTTTAATATTACGGCGCCTTGGAATAGTCAGAACACCGATGGCATCCATATTAGTAACTCCACCAATATCAACGTTGCCAATTCCCACATCGGAGTTGGTGACGATTGTGTTTCCATTGGACCGGGTAGCTTCGATATATTAGTTTCGAATATTAGATGTGGCCCAGGTCATGGAATCAGCGTTGGGAGCCTAGGGAGGTACAAGAACGAGATGGATGTGATAGGAATAAGAGTGGAGAATTGTACCATAAGTGATACTCAAAATGGAGTGAGGGTAAAGACATGGCCGGGAAATTATTCGAGTAATGCTGCTCATTTGACGTTTCGAAACATTGTGATGAACAATGTCTCAAATCCCATTATCATAGATCAACGTTATTGTCCTAACCATTCTTGTGACATGAAAGAGTTAGATATCGGGCGGTGTGTCAGCGAGAATGCTGGCCCCAAGGAGGATGGATTGCGAGATCCCACATTAGTGGAAGAGGGGAACGAAACATTCCTTATAAGGTCATCAAGTGTGAAGCTGAGTGAGATGAACATGAGAAACATCAGTGGAACTTACAACACAAAGTTGGCAGTAAACATAGACTGCAGTTCAACCATGCCTTGTAATAACATTCACCTTTCATACATCAATCTCACCGCCACTCACCCACCGGAGAAGTATGAACTTGGGCGCTTCAATTGCATGGGTGCTCTCAATACCTTCACAGTTCTTAATTCAACATTTTAG

Protein sequence

MKLSPFLFSFLLALSVSASTFSPDTPLESADGGFDSLALEPSGEFPLSGRGVFNVNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF
Homology
BLAST of Carg19649 vs. NCBI nr
Match: KAG7018595.1 (Exopolygalacturonase-like 6, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 933.7 bits (2412), Expect = 6.0e-268
Identity = 457/457 (100.00%), Postives = 457/457 (100.00%), Query Frame = 0

Query: 1   MKLSPFLFSFLLALSVSASTFSPDTPLESADGGFDSLALEPSGEFPLSGRGVFNVNDYGA 60
           MKLSPFLFSFLLALSVSASTFSPDTPLESADGGFDSLALEPSGEFPLSGRGVFNVNDYGA
Sbjct: 1   MKLSPFLFSFLLALSVSASTFSPDTPLESADGGFDSLALEPSGEFPLSGRGVFNVNDYGA 60

Query: 61  VADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATL 120
           VADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATL
Sbjct: 61  VADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATL 120

Query: 121 IAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKL 180
           IAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKL
Sbjct: 121 IAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKL 180

Query: 181 TDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVAN 240
           TDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVAN
Sbjct: 181 TDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVAN 240

Query: 241 SHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQ 300
           SHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQ
Sbjct: 241 SHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQ 300

Query: 301 NGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENA 360
           NGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENA
Sbjct: 301 NGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENA 360

Query: 361 GPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNI 420
           GPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNI
Sbjct: 361 GPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNI 420

Query: 421 HLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF 458
           HLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF
Sbjct: 421 HLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF 457

BLAST of Carg19649 vs. NCBI nr
Match: XP_022956245.1 (exopolygalacturonase-like [Cucurbita moschata])

HSP 1 Score: 704.1 bits (1816), Expect = 7.7e-199
Identity = 345/385 (89.61%), Postives = 349/385 (90.65%), Query Frame = 0

Query: 73  MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 132
           MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI
Sbjct: 1   MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 60

Query: 133 HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 192
           HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS
Sbjct: 61  HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 120

Query: 193 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 252
           LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI
Sbjct: 121 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 180

Query: 253 GPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 312
           GPGSFD+LVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY
Sbjct: 181 GPGSFDVLVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 240

Query: 313 SSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRDPTL 372
           SSNAAHLTF+NIVMNNVSNPIIIDQRYCPNHSCDMKE                       
Sbjct: 241 SSNAAHLTFQNIVMNNVSNPIIIDQRYCPNHSCDMKE----------------------- 300

Query: 373 VEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINLTATHP 432
                      SSSVKLSEMNMRNISGTYNTK AVNIDCSS MPCNN+HLSYINLTATHP
Sbjct: 301 -----------SSSVKLSEMNMRNISGTYNTKFAVNIDCSSIMPCNNVHLSYINLTATHP 351

Query: 433 PEKYELGRFNCMGALNTFTVLNSTF 458
           PEKYE+GRFNCMGALNTFTVLNSTF
Sbjct: 361 PEKYEIGRFNCMGALNTFTVLNSTF 351

BLAST of Carg19649 vs. NCBI nr
Match: XP_023539060.1 (exopolygalacturonase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 587.0 bits (1512), Expect = 1.4e-163
Identity = 297/464 (64.01%), Postives = 350/464 (75.43%), Query Frame = 0

Query: 1   MKLSP-FLFSFLLALSVSASTFSPDTPLESADGGFDSLAL-----EPSGEFPLSGRGVFN 60
           MKLS   +F FLLA+SVS   FSP+  LESA G +D L L     EP    P  G  VF+
Sbjct: 1   MKLSALIIFYFLLAVSVSGRNFSPEPALESAAGSWDFLLLPPDMAEPPVGDPWRGLAVFD 60

Query: 61  VNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKV 120
           V DYGA+ADGKTENSLAFM AWE+ACA++G S  LIPEG+F +A V FSGPCF +LSPKV
Sbjct: 61  VTDYGAIADGKTENSLAFMGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDLSPKV 120

Query: 121 LIFATLIAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIF 180
           +I  TLIAPT LT DVWI+F SLRHL +TG  GS +L+GQGA+TW +G  CR R++C IF
Sbjct: 121 IILGTLIAPTTLTADVWIYFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLSCGIF 180

Query: 181 TTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNST 240
           TTSLKL+DVS+A V +ISLV+SKGFHVA++GCD+IHL GFNITAPWNSQNTDGIHIS ST
Sbjct: 181 TTSLKLSDVSNATVSNISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHISQST 240

Query: 241 NINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENC 300
           NIN+  S I VGDDCVSIGPGS ++ VSN+RCGPGHGISVGSLGRY+ E DV+GIRVENC
Sbjct: 241 NINITTSDISVGDDCVSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVMGIRVENC 300

Query: 301 TISDTQNGVRVKTWP-GNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIG 360
           TI DTQNGVRVKTWP G+++S A +LTF+NIVM NVSNPIIIDQ+YCPNH CD+KE    
Sbjct: 301 TIKDTQNGVRVKTWPGGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHFCDIKE---- 360

Query: 361 RCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSS 420
                                         SS VKLS +N++NISGTYN++ AVN+ CSS
Sbjct: 361 ------------------------------SSGVKLSNINIKNISGTYNSEFAVNLQCSS 420

Query: 421 TMPCNNIHLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF 458
            MPC N+ LSYINLTA  PPE  ++GRFNC G+LN F++LNSTF
Sbjct: 421 AMPCENVQLSYINLTAIEPPETDDIGRFNCKGSLNNFSILNSTF 430

BLAST of Carg19649 vs. NCBI nr
Match: KAG7028995.1 (Exopolygalacturonase-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 585.9 bits (1509), Expect = 3.1e-163
Identity = 297/464 (64.01%), Postives = 350/464 (75.43%), Query Frame = 0

Query: 1   MKLSPF-LFSFLLALSVSASTFSPDTPLESADGGFDSLAL-----EPSGEFPLSGRGVFN 60
           MKLS   +F FLLA+SVS   FSP+  LESA G +D L L     EP    P  G  VF+
Sbjct: 1   MKLSSLVMFYFLLAVSVSGRNFSPEPALESAAGSWDFLLLPPDMAEPPVGDPWRGLAVFD 60

Query: 61  VNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKV 120
           V DYGA+ADGKTENSLAFM AWE+ACA++G S  LIPEG+F +A V FSGPCF +LSPKV
Sbjct: 61  VTDYGAIADGKTENSLAFMGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDLSPKV 120

Query: 121 LIFATLIAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIF 180
           +I  TLIAPT LT DVWI+F SLRHL +TG  GS +L+GQGA+TW +G  CR R++C IF
Sbjct: 121 IILGTLIAPTTLTADVWIYFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLSCGIF 180

Query: 181 TTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNST 240
           TTSLKL+DVS+A V +ISLV+SKGFHVA++GCD+IHL GFNITAPWNSQNTDGIHIS ST
Sbjct: 181 TTSLKLSDVSNATVSNISLVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHISQST 240

Query: 241 NINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENC 300
           NIN+  S I VGDDCVSIGPGS ++ VSN+RCGPGHGISVGSLGRY+ E DVIGIRVENC
Sbjct: 241 NINITASDISVGDDCVSIGPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVIGIRVENC 300

Query: 301 TISDTQNGVRVKTWP-GNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIG 360
           TI DTQNGVRVKTWP G+++S A +LTF+NIVM NVSNPIIIDQ+YCPNH CD+KE    
Sbjct: 301 TIKDTQNGVRVKTWPGGDHASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCDIKE---- 360

Query: 361 RCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSS 420
                                         SS VKLS +N++NISGTYN++ AVN+ CSS
Sbjct: 361 ------------------------------SSGVKLSNINIKNISGTYNSEFAVNLQCSS 420

Query: 421 TMPCNNIHLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF 458
            +PC N+ LSYINLTA  PPE  ++GRFNC G+LN F++LNSTF
Sbjct: 421 AVPCENVQLSYINLTAIEPPETDDIGRFNCKGSLNNFSILNSTF 430

BLAST of Carg19649 vs. NCBI nr
Match: XP_038885913.1 (exopolygalacturonase-like [Benincasa hispida])

HSP 1 Score: 576.6 bits (1485), Expect = 1.9e-160
Identity = 294/463 (63.50%), Postives = 345/463 (74.51%), Query Frame = 0

Query: 1   MKLSPFLFSFLLALSVSASTFSPDTPLESADGGFDSL------ALEPSGEFPLSGRGVFN 60
           MKLS  +FSFLLALS+SA  F       SA G FD L      A  P+G+ P  G  VF+
Sbjct: 1   MKLSLLVFSFLLALSLSARNF-------SAAGSFDFLLPPSYSAESPAGD-PWMGLRVFD 60

Query: 61  VNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKV 120
           VNDYGA+ADGKTENSLAFM+AWE+AC + G S+ LIPEG F V  VTFSGPCFN++SPKV
Sbjct: 61  VNDYGAIADGKTENSLAFMSAWEDACGYNGSSMVLIPEGNFFVDQVTFSGPCFNDISPKV 120

Query: 121 LIFATLIAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIF 180
           LI  TLIAPT L  D WI F SLR  +LTG + SA+L+GQGA TWS GSACR RMTC  F
Sbjct: 121 LILGTLIAPTSLADDFWIRFQSLRRFILTGGESSAILDGQGAPTWSSGSACRHRMTCDAF 180

Query: 181 TTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNST 240
            TSLKL+++S+ IV +I LVDSKGFHV+++GCD+IHL GFNITAPW+SQNTDGIHIS ST
Sbjct: 181 ATSLKLSNISNGIVNNIRLVDSKGFHVSIYGCDNIHLSGFNITAPWDSQNTDGIHISQST 240

Query: 241 NINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENC 300
           NI++  S IGVGDDCVS+GPG  ++ +S+IRCGPGHGISVGSLG+YKNE DV+GIRVENC
Sbjct: 241 NISITISDIGVGDDCVSLGPGCVNVSISDIRCGPGHGISVGSLGKYKNENDVMGIRVENC 300

Query: 301 TISDTQNGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGR 360
           TI+ TQNGVRVKTWPG+  SNA +LTF+NIVM NVSNPIIIDQ YCPNHSCD +E     
Sbjct: 301 TINGTQNGVRVKTWPGDLVSNATNLTFQNIVMINVSNPIIIDQHYCPNHSCDPQE----- 360

Query: 361 CVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSST 420
                                        SSSVKLS++N+RNISGTYNT+ AVNI CS+T
Sbjct: 361 -----------------------------SSSVKLSDINIRNISGTYNTEFAVNIQCSTT 420

Query: 421 MPCNNIHLSYINLTATHPPEKYELGRFNCMGALNTFTVLNSTF 458
            PC N  LSYINLTA  PP+ +++G FNC GALN+FT+ NSTF
Sbjct: 421 KPCENFQLSYINLTAIQPPQTHKIGHFNCKGALNSFTIFNSTF 421

BLAST of Carg19649 vs. ExPASy Swiss-Prot
Match: Q6H9K0 (Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 SV=1)

HSP 1 Score: 273.9 bits (699), Expect = 3.4e-72
Identity = 150/383 (39.16%), Postives = 210/383 (54.83%), Query Frame = 0

Query: 48  SGRGVFNVNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCF 107
           S   VFNVNDYG  A G  + S A M AW+ ACA +G S  LIP+G + +  V   GPC 
Sbjct: 5   SSGSVFNVNDYG--AKGAGDISQAVMKAWKAACASQGPSTVLIPKGNYNMGEVAMQGPCK 64

Query: 108 NNLSPKVL--IFATLIAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSAC 167
            +     +  +      P+K  +D W+ F+ +  L ++   G+  L+GQG   W+  + C
Sbjct: 65  GSKIGFQIDGVVKAPADPSKFKSDGWVSFYRIDGLTVS---GTGTLDGQGQTAWAKNN-C 124

Query: 168 RRRMTCPIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNT 227
            +   C     +L+   + HA+V+DI+ ++SK FH+ +  C+ I      +TAP  S NT
Sbjct: 125 DKNPNCKHAAMNLRFDFLKHAMVRDITSLNSKMFHINVLECEDITFQHVTVTAPGTSINT 184

Query: 228 DGIHISNSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMD 287
           DGIH+  S  + + N+ I  GDDC+SIGPGS ++ ++ + CGPGHGIS+GSLGRY NE +
Sbjct: 185 DGIHVGISKGVTITNTKIATGDDCISIGPGSQNVTITQVNCGPGHGISIGSLGRYNNEKE 244

Query: 288 VIGIRVENCTISDTQNGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSC 347
           V GI V+ CT S T NGVRVKTWP +    A  LTF+++ MNNV NP+I+DQ YCP   C
Sbjct: 245 VRGITVKGCTFSGTMNGVRVKTWPNSPPGAATDLTFQDLTMNNVQNPVILDQEYCPYGQC 304

Query: 348 DMKELDIGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKL 407
             +                                    S +KLS +N  NI GT   K+
Sbjct: 305 SRQ----------------------------------APSRIKLSNINFNNIRGTSTGKV 347

Query: 408 AVNIDCSSTMPCNNIHLSYINLT 429
           AV I CS  MPC+N+ +  INL+
Sbjct: 365 AVVIACSHGMPCSNMKIGEINLS 347

BLAST of Carg19649 vs. ExPASy Swiss-Prot
Match: P24548 (Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1)

HSP 1 Score: 266.5 bits (680), Expect = 5.4e-70
Identity = 142/362 (39.23%), Postives = 208/362 (57.46%), Query Frame = 0

Query: 67  ENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKL 126
           +++ A   AW+EACA    S  L+P+G F V  +T  GPC +++   + +  TL AP   
Sbjct: 1   DSTQALTTAWKEACASASPSTILVPKGNFAVGLITLEGPCKSSIG--LQLQGTLKAPADP 60

Query: 127 TTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHA 186
           +    + + +L  + L  + G  V +GQG   W      +    C   + +L+L  V+++
Sbjct: 61  SKIKGLGWINLNKIDLLTIFGGGVFDGQGKSAWVQNDCHKNGPICKTLSMNLRLYAVTNS 120

Query: 187 IVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVG 246
           I++D++ +DSK FHV + GC ++    F I+A   S NTDGIHI  S  +N+ N+ I  G
Sbjct: 121 ILRDVTTLDSKNFHVNVIGCKNLTFERFKISAAETSINTDGIHIGRSDGVNIINTEIKTG 180

Query: 247 DDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVK 306
           DDC+S+G GS +I ++NI CGPGHGISVGSLGRYKNE  V+GI V+NCTI+ +QNGVR+K
Sbjct: 181 DDCISLGDGSKNINITNITCGPGHGISVGSLGRYKNEESVVGIYVKNCTITGSQNGVRIK 240

Query: 307 TWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDG 366
           TWP +    A+ + F++I MN+V  PI+IDQ YCP + C  +                  
Sbjct: 241 TWPKSEPGEASEMHFQDITMNSVGTPILIDQGYCPYNQCTAEV----------------- 300

Query: 367 LRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYIN 426
                             SSVKLS+++ +NI GT  TK AV + CS + PCN + L+ I+
Sbjct: 301 -----------------PSSVKLSKISFKNIKGTSTTKEAVKLVCSKSFPCNGVELADID 326

Query: 427 LT 429
           LT
Sbjct: 361 LT 326

BLAST of Carg19649 vs. ExPASy Swiss-Prot
Match: P49062 (Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 SV=1)

HSP 1 Score: 255.4 bits (651), Expect = 1.2e-66
Identity = 140/377 (37.14%), Postives = 209/377 (55.44%), Query Frame = 0

Query: 52  VFNVNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLS 111
           V+++  +GAV DG T    AF+  W + C     +  L+P+GTFL   V F+GPC + ++
Sbjct: 51  VYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVT 110

Query: 112 PKVLIFATLIAPTK-LTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMT 171
             V +  T+IA T    T  W  F  + +L+LT   G+   +G+G   W     C +++ 
Sbjct: 111 --VNVIGTIIATTSGYATPEWFLFERVDNLVLT---GTGTFHGKGEAVWK-ADGCGKKVQ 170

Query: 172 CPIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHI 231
           C +  TSLK  ++ +  +  IS V++K FH+ +   +++++    +TAP  S NTDGIH+
Sbjct: 171 CNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHL 230

Query: 232 SNSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIR 291
           SN+ N+++ +S I  GDDCVS+G GS ++ V  + CGPGHG+SVGSLG+YKNE DV GI 
Sbjct: 231 SNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIH 290

Query: 292 VENCTISDTQNGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKEL 351
           V NCT+ +T NG+R+KTW G+  S A  + F NI+M +V NPIIIDQ Y           
Sbjct: 291 VNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNY----------- 350

Query: 352 DIGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNID 411
                          G R                S V +S++  +NI GT  TK  V I 
Sbjct: 351 ---------------GSRG-------------GDSQVAISDILFKNIRGTTITKDVVQIM 382

Query: 412 CSSTMPCNNIHLSYINL 428
           CS ++PC  +++  +NL
Sbjct: 411 CSKSVPCQGVNVVDVNL 382

BLAST of Carg19649 vs. ExPASy Swiss-Prot
Match: Q39766 (Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1)

HSP 1 Score: 252.3 bits (643), Expect = 1.1e-65
Identity = 137/376 (36.44%), Postives = 208/376 (55.32%), Query Frame = 0

Query: 55  VNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKV 114
           V  +GA ADGKT+ S  F+ AW+EACA    S  +IP+GT+L++ V   GPC      ++
Sbjct: 32  VAKFGAKADGKTDLSKPFLDAWKEACASVTPSTVVIPKGTYLLSKVNLEGPC--KAPIEI 91

Query: 115 LIFATLIA---PTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTC 174
            +  T+ A   P+      W+ F+S+ +  +    G  + +GQG+  +   + C  R   
Sbjct: 92  NVQGTIQAPADPSAFKDPNWVRFYSVENFKMF---GGGIFDGQGSIAYE-KNTCENREFR 151

Query: 175 PIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHIS 234
                +++   V++A+++DI+  DSK FH+ +  C +I L    I AP  S NTDGIH+ 
Sbjct: 152 SKLPVNIRFDFVTNALIQDITSKDSKLFHINVFACKNITLERLKIEAPDESPNTDGIHMG 211

Query: 235 NSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRV 294
            S  +N+  S I  GDDC+SIG G+ ++++  I CGPGHGIS+GSLG+++NE  V GI++
Sbjct: 212 KSEGVNIIASDIKTGDDCISIGDGTKNMVIKEITCGPGHGISIGSLGKFQNEEPVEGIKI 271

Query: 295 ENCTISDTQNGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELD 354
            NCTI++T NG R+KTWPG +    + + F +I MNNVS+PI+IDQ+YCP + C   E  
Sbjct: 272 SNCTITNTSNGARIKTWPGEHGGAVSEIHFEDITMNNVSSPILIDQQYCPWNKCKKNE-- 331

Query: 355 IGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDC 414
                                            S VKLS ++ +NI GT     A+   C
Sbjct: 332 --------------------------------ESKVKLSNISFKNIRGTSALPEAIKFIC 367

Query: 415 SSTMPCNNIHLSYINL 428
           S + PC N+ L+ I++
Sbjct: 392 SGSSPCQNVELADIDI 367

BLAST of Carg19649 vs. ExPASy Swiss-Prot
Match: P49063 (Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 PE=2 SV=2)

HSP 1 Score: 251.5 bits (641), Expect = 1.8e-65
Identity = 156/418 (37.32%), Postives = 222/418 (53.11%), Query Frame = 0

Query: 17  SASTFSPDTPLESA---DGGFDSLALEPSGEFPLSGRGVFNVNDYGAVADGKTENSLAFM 76
           +A+T   DT   +A    G   S+  + SG  P  G  V +V   GA  D KT++S AF 
Sbjct: 34  NAATAVEDTAKNAATAVGGAAASVGAKVSGAKP--GAAV-DVKASGAKGDSKTDDSAAFA 93

Query: 77  AAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDV--- 136
           AAW+EACA    S   +P+G ++V  + F GPC   ++  + +     AP  + T     
Sbjct: 94  AAWKEACA--AGSTITVPKGEYMVESLEFKGPCKGPVT--LELNGNFKAPATVKTTKPHA 153

Query: 137 -WIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVK 196
            WI F ++    L G    A+ +GQG+  W   + C +   C     +++ T ++++ + 
Sbjct: 154 GWIDFENIADFTLNG--NKAIFDGQGSLAWK-ANDCAKTGKCNSLPINIRFTGLTNSKIN 213

Query: 197 DISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDC 256
            I+  +SK FH+ +  C +I L    I AP  S NTDGIHI  S  +N+  + I  GDDC
Sbjct: 214 SITSTNSKLFHMNILNCKNITLSDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDC 273

Query: 257 VSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWP 316
           VSIG G+ +++V N+ CGPGHGIS+GSLGRY NE  V G+ V  C I +T NGVR+KTWP
Sbjct: 274 VSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWP 333

Query: 317 GNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRD 376
           G+    A+++ F +I M+NVS P++IDQ YCP   C              AG        
Sbjct: 334 GSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHC-------------KAGV------- 393

Query: 377 PTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINL 428
                          S VKLS++ ++ I GT  TK+AV + CS  +PC NI LS INL
Sbjct: 394 --------------PSQVKLSDVTIKGIKGTSATKVAVKLMCSKGVPCTNIALSDINL 407

BLAST of Carg19649 vs. ExPASy TrEMBL
Match: A0A6J1GXB0 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457998 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 3.7e-199
Identity = 345/385 (89.61%), Postives = 349/385 (90.65%), Query Frame = 0

Query: 73  MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 132
           MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI
Sbjct: 1   MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 60

Query: 133 HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 192
           HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS
Sbjct: 61  HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 120

Query: 193 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 252
           LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI
Sbjct: 121 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 180

Query: 253 GPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 312
           GPGSFD+LVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY
Sbjct: 181 GPGSFDVLVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 240

Query: 313 SSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRDPTL 372
           SSNAAHLTF+NIVMNNVSNPIIIDQRYCPNHSCDMKE                       
Sbjct: 241 SSNAAHLTFQNIVMNNVSNPIIIDQRYCPNHSCDMKE----------------------- 300

Query: 373 VEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINLTATHP 432
                      SSSVKLSEMNMRNISGTYNTK AVNIDCSS MPCNN+HLSYINLTATHP
Sbjct: 301 -----------SSSVKLSEMNMRNISGTYNTKFAVNIDCSSIMPCNNVHLSYINLTATHP 351

Query: 433 PEKYELGRFNCMGALNTFTVLNSTF 458
           PEKYE+GRFNCMGALNTFTVLNSTF
Sbjct: 361 PEKYEIGRFNCMGALNTFTVLNSTF 351

BLAST of Carg19649 vs. ExPASy TrEMBL
Match: A0A6J1ITM7 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111479249 PE=3 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.8e-156
Identity = 267/279 (95.70%), Postives = 273/279 (97.85%), Query Frame = 0

Query: 73  MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 132
           M+AWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI
Sbjct: 1   MSAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 60

Query: 133 HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 192
           HFHSLRHLLLTG DGS VLNGQGAQTWSFGSACR RMTCPIFTTSLKLT VSHAIVK+IS
Sbjct: 61  HFHSLRHLLLTGFDGSTVLNGQGAQTWSFGSACRHRMTCPIFTTSLKLTGVSHAIVKNIS 120

Query: 193 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 252
           LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI
Sbjct: 121 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 180

Query: 253 GPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 312
           GPGSFD+LVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY
Sbjct: 181 GPGSFDVLVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPGNY 240

Query: 313 SSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELD 352
           SSNAAHLTF+NIVMNNVSNPIIIDQ YCPNHSCD KE++
Sbjct: 241 SSNAAHLTFQNIVMNNVSNPIIIDQHYCPNHSCDTKEVN 279

BLAST of Carg19649 vs. ExPASy TrEMBL
Match: A0A6J1FMM1 (exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446788 PE=3 SV=1)

HSP 1 Score: 519.2 bits (1336), Expect = 1.7e-143
Identity = 252/386 (65.28%), Postives = 299/386 (77.46%), Query Frame = 0

Query: 73  MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 132
           M AWE+ACA++G S  LIPEG+F +A V FSGPCF +LSPKV+I  TLIAPT LT DVWI
Sbjct: 1   MGAWEDACAYRGSSTLLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLTADVWI 60

Query: 133 HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 192
           +F SLRHL +TG  GS +L+GQGA+TW +G  CR R++C IFTTSLKL+DVS+A V +IS
Sbjct: 61  YFKSLRHLTITGNVGSGILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVSNIS 120

Query: 193 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 252
           LV+SKGFHVA++GCD+IHL GFNITAPWNSQNTDGIHIS STNIN+  S I VGDDCVSI
Sbjct: 121 LVNSKGFHVALNGCDNIHLSGFNITAPWNSQNTDGIHISQSTNINITASDISVGDDCVSI 180

Query: 253 GPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWP-GN 312
           GPGS ++ VSN+RCGPGHGISVGSLGRY+ E DVIGIRVENCTI DTQNGVRVKTWP G+
Sbjct: 181 GPGSINVSVSNVRCGPGHGISVGSLGRYREEKDVIGIRVENCTIKDTQNGVRVKTWPGGD 240

Query: 313 YSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRDPT 372
           ++S A +LTF+NIVM NVSNPIIIDQ+YCPNH CD+KE                      
Sbjct: 241 HASKATNLTFQNIVMFNVSNPIIIDQQYCPNHLCDIKE---------------------- 300

Query: 373 LVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINLTATH 432
                       SS VKLS +N++NISGTYN++ AVN+ CSS +PC N+ LSYINLTA  
Sbjct: 301 ------------SSGVKLSNINIKNISGTYNSEFAVNLQCSSAVPCENVQLSYINLTAIE 352

Query: 433 PPEKYELGRFNCMGALNTFTVLNSTF 458
           PPE  ++GRFNC G+LN F++LNSTF
Sbjct: 361 PPETDDIGRFNCKGSLNNFSILNSTF 352

BLAST of Carg19649 vs. ExPASy TrEMBL
Match: A0A0A0L932 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164560 PE=3 SV=1)

HSP 1 Score: 514.6 bits (1324), Expect = 4.2e-142
Identity = 277/464 (59.70%), Postives = 322/464 (69.40%), Query Frame = 0

Query: 7   LFSFLLALSVSASTFSPDTPLESADGGFD-----SLALEPSGEFPLSGRGVFNVNDYGAV 66
           LFS LLA+++SA+             GFD     S   EP    P SG  VF+VNDYG V
Sbjct: 9   LFSSLLAITLSAA------------DGFDIALPPSYPAEPPVGDPWSGLRVFDVNDYGGV 68

Query: 67  ADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLI 126
           ADGKT NSLA MAAWE+AC  +G S+ LIPEG + V  VTFSGPCFN+LSPKVLI  TLI
Sbjct: 69  ADGKTNNSLALMAAWEDACYHQGGSMLLIPEGNYFVDQVTFSGPCFNDLSPKVLILGTLI 128

Query: 127 APTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLT 186
           APT LT DVWIHF SLR L LT     + L+G+GAQTWS GS CR  MTC +F  SLKL+
Sbjct: 129 APTNLTADVWIHFDSLRRLSLTA-GHLSTLDGRGAQTWSSGSRCRHAMTCAMFAISLKLS 188

Query: 187 DVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANS 246
           +V +  V  I+LVDSK FHV++H CD+IHL GFNITAPW SQNTDG HIS STNI++ NS
Sbjct: 189 NVLNGTVSSINLVDSKAFHVSIHQCDNIHLSGFNITAPWYSQNTDGFHISQSTNISITNS 248

Query: 247 HIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQN 306
            IGVGDDCVSIGPGS ++LVSN+ CGPGHGIS+GSLGRYK E DV GI VENCTIS TQN
Sbjct: 249 KIGVGDDCVSIGPGSVNVLVSNVTCGPGHGISIGSLGRYKMENDVRGIWVENCTISGTQN 308

Query: 307 GVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAG 366
           GVRVKTWPG+++SNA +LTF+NIVM NVSNPIIIDQ+YCPN SCD +E            
Sbjct: 309 GVRVKTWPGDHASNATNLTFQNIVMINVSNPIIIDQQYCPNSSCDSRE------------ 368

Query: 367 PKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIH 426
                                 SSSVKLS++N+RNISGTYN++ A+N+ CSST  C NI 
Sbjct: 369 ----------------------SSSVKLSDINIRNISGTYNSEFAINMKCSSTKACENIR 425

Query: 427 LSYINLTA-THPPEKYE-------LGRFNCMGALNTFTVLNSTF 458
           LSYINLT+  H  +  E        G FN  GALN+FT+ NSTF
Sbjct: 429 LSYINLTSINHQSDDDEDEEEGDVTGNFNFKGALNSFTIFNSTF 425

BLAST of Carg19649 vs. ExPASy TrEMBL
Match: A0A6J1I855 (exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470865 PE=3 SV=1)

HSP 1 Score: 513.5 bits (1321), Expect = 9.3e-142
Identity = 250/386 (64.77%), Postives = 297/386 (76.94%), Query Frame = 0

Query: 73  MAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDVWI 132
           M AWE+ACA++G S FLIPEG+F +A V FSGPCF +LSPKV+I  TLIAPT LT DVWI
Sbjct: 1   MGAWEDACAYRGSSTFLIPEGSFFLAQVLFSGPCFGDLSPKVIILGTLIAPTTLTADVWI 60

Query: 133 HFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVKDIS 192
           +F SLR L +TG  GS +L+GQGA+TW +G  CR R++C IFTTSLKL+DVS+A V +IS
Sbjct: 61  YFKSLRQLTITGNIGSGILDGQGAETWLYGFNCRHRLSCGIFTTSLKLSDVSNATVSNIS 120

Query: 193 LVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDCVSI 252
           LV+SKGFHVA++GCD+IHL GF ITAPWNSQNTDGIHIS STNIN+  S I VGDDCVSI
Sbjct: 121 LVNSKGFHVALNGCDNIHLSGFKITAPWNSQNTDGIHISQSTNINITASDISVGDDCVSI 180

Query: 253 GPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWPG-N 312
           GPGS ++ VSN+ CGPGHGISVGSLGRY+ E DV+GIRVENCTI DTQNGVRVKTWPG +
Sbjct: 181 GPGSINVSVSNVICGPGHGISVGSLGRYREEKDVMGIRVENCTIKDTQNGVRVKTWPGED 240

Query: 313 YSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRDPT 372
           ++S A +LTF+NIVM NVSNPIIIDQ+YCPNH CD+KE                      
Sbjct: 241 HASKATNLTFQNIVMFNVSNPIIIDQQYCPNHFCDIKE---------------------- 300

Query: 373 LVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINLTATH 432
                       SS VKLS +N++NISGTYN++ AVN+ CSS MPC N+ LSYINLTA  
Sbjct: 301 ------------SSGVKLSNINIKNISGTYNSEFAVNLQCSSAMPCENVQLSYINLTAIE 352

Query: 433 PPEKYELGRFNCMGALNTFTVLNSTF 458
           PPE  ++GRFNC G+LN F++LNSTF
Sbjct: 361 PPETDDIGRFNCKGSLNNFSILNSTF 352

BLAST of Carg19649 vs. TAIR 10
Match: AT4G18180.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 272.7 bits (696), Expect = 5.3e-73
Identity = 154/376 (40.96%), Postives = 212/376 (56.38%), Query Frame = 0

Query: 54  NVNDYGAVADGKTENSLAFMAAWEEACAFKGDSI-FLIPEGTFLVAGVTFSGPCFNNLSP 113
           +V  +GA A+   +++ AF+AAW++AC     S+  +IP G F V  + FSGPC N  + 
Sbjct: 36  DVRSFGARANDHRDHTKAFVAAWDKACKSSSSSVNLIIPRGEFSVGSLRFSGPCTNVSNL 95

Query: 114 KVLIFATLIAPTKLTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCP 173
            V + A+       +   WI F  +  L LT   G    +GQGA  W F + C     C 
Sbjct: 96  TVRVKASTDLSKYRSGGGWIQFGWINGLTLT---GGGTFDGQGALAWPFNN-CTSDSNCK 155

Query: 174 IFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISN 233
           +  TSLK   ++  +V+ IS V+SK FH+A+  C        NITAP +S NTDGIHI  
Sbjct: 156 LLPTSLKFVGMNRTVVRRISSVNSKFFHIALVECRDFKGTRLNITAPSDSPNTDGIHIER 215

Query: 234 STNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVE 293
           S+N+  + SHI  GDDCVSIG G+  I +++I+CGPGHGISVGSLGRY NE DV G+ V+
Sbjct: 216 SSNVYFSRSHIATGDDCVSIGQGNSQITITSIKCGPGHGISVGSLGRYPNEKDVNGLVVK 275

Query: 294 NCTISDTQNGVRVKTWPGNYSSNAA-HLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELD 353
           +C IS T NG+R+KTW  +   +AA ++TF NI+MNNV+NPIIIDQ YCP  SC      
Sbjct: 276 DCKISGTTNGIRIKTWANSPGLSAATNMTFENIIMNNVTNPIIIDQSYCPFSSCISNV-- 335

Query: 354 IGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDC 413
                                            S V+LSE+  +NI GT ++ +AV + C
Sbjct: 336 --------------------------------PSKVELSEIYFKNIRGTSSSLVAVQLHC 373

Query: 414 SSTMPCNNIHLSYINL 428
           S  MPC  ++L  ++L
Sbjct: 396 SRGMPCKKVYLENVHL 373

BLAST of Carg19649 vs. TAIR 10
Match: AT3G14040.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 258.5 bits (659), Expect = 1.0e-68
Identity = 158/418 (37.80%), Postives = 223/418 (53.35%), Query Frame = 0

Query: 17  SASTFSPDTPLESA---DGGFDSLALEPSGEFPLSGRGVFNVNDYGAVADGKTENSLAFM 76
           +A+T   DT   +A    G   S+  + +G  P  G    +V   GA  DGKT++S AF 
Sbjct: 34  NAATAVEDTAKNAATAVGGAAASVGAKVTGAKP--GGASLDVKASGAKGDGKTDDSAAFA 93

Query: 77  AAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLSPKVLIFATLIAPTKLTTDV--- 136
           AAW+EACA    S   +P+G +LV  + F GPC   ++  + +     AP  + T     
Sbjct: 94  AAWKEACA--AGSTITVPKGEYLVESLEFKGPCKGPVT--LELNGNFKAPATVKTTKPHA 153

Query: 137 -WIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMTCPIFTTSLKLTDVSHAIVK 196
            WI F +L    L G    A+ +GQG+  W   + C +   C     +++ T ++++ + 
Sbjct: 154 GWIDFENLADFTLNG--NKAIFDGQGSLAWK-ANDCAKTGKCNSLPINIRFTGLTNSKIN 213

Query: 197 DISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHISNSTNINVANSHIGVGDDC 256
            I+  +SK FH+ +  C +I L    I AP  S NTDGIHI  S  +N+  + I  GDDC
Sbjct: 214 SITSTNSKLFHMNILNCKNITLTDIGIDAPPESLNTDGIHIGRSNGVNLIGAKIKTGDDC 273

Query: 257 VSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIRVENCTISDTQNGVRVKTWP 316
           VSIG G+ +++V N+ CGPGHGIS+GSLGRY NE  V G+ V  C I +T NGVR+KTWP
Sbjct: 274 VSIGDGTENLIVENVECGPGHGISIGSLGRYPNEQPVKGVTVRKCLIKNTDNGVRIKTWP 333

Query: 317 GNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKELDIGRCVSENAGPKEDGLRD 376
           G+    A+++ F +I M+NVS P++IDQ YCP   C              AG        
Sbjct: 334 GSPPGIASNILFEDITMDNVSLPVLIDQEYCPYGHC-------------KAGV------- 393

Query: 377 PTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNIDCSSTMPCNNIHLSYINL 428
                          S VKLS++ ++NI GT  TK+AV + CS  +PC NI LS INL
Sbjct: 394 --------------PSKVKLSDVTIKNIKGTSATKVAVKLMCSKGVPCTNIALSDINL 408

BLAST of Carg19649 vs. TAIR 10
Match: AT1G02790.1 (polygalacturonase 4 )

HSP 1 Score: 255.4 bits (651), Expect = 8.8e-68
Identity = 140/377 (37.14%), Postives = 209/377 (55.44%), Query Frame = 0

Query: 52  VFNVNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGPCFNNLS 111
           V+++  +GAV DG T    AF+  W + C     +  L+P+GTFL   V F+GPC + ++
Sbjct: 51  VYDITKFGAVGDGSTNTFKAFLNTWIQVCDSPVPATLLVPKGTFLAGPVIFAGPCKSKVT 110

Query: 112 PKVLIFATLIAPTK-LTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRRRMT 171
             V +  T+IA T    T  W  F  + +L+LT   G+   +G+G   W     C +++ 
Sbjct: 111 --VNVIGTIIATTSGYATPEWFLFERVDNLVLT---GTGTFHGKGEAVWK-ADGCGKKVQ 170

Query: 172 CPIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDGIHI 231
           C +  TSLK  ++ +  +  IS V++K FH+ +   +++++    +TAP  S NTDGIH+
Sbjct: 171 CNLPPTSLKFRNMKNVEINGISSVNAKAFHMFLVKTENVNIQNIKLTAPAESPNTDGIHL 230

Query: 232 SNSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVIGIR 291
           SN+ N+++ +S I  GDDCVS+G GS ++ V  + CGPGHG+SVGSLG+YKNE DV GI 
Sbjct: 231 SNADNVSILDSTIATGDDCVSVGRGSNNVTVERVICGPGHGLSVGSLGKYKNEEDVSGIH 290

Query: 292 VENCTISDTQNGVRVKTWPGNYSSNAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCDMKEL 351
           V NCT+ +T NG+R+KTW G+  S A  + F NI+M +V NPIIIDQ Y           
Sbjct: 291 VNNCTMIETDNGLRIKTWGGSDPSKAVDIKFENIIMQSVKNPIIIDQNY----------- 350

Query: 352 DIGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLAVNID 411
                          G R                S V +S++  +NI GT  TK  V I 
Sbjct: 351 ---------------GSRG-------------GDSQVAISDILFKNIRGTTITKDVVQIM 382

Query: 412 CSSTMPCNNIHLSYINL 428
           CS ++PC  +++  +NL
Sbjct: 411 CSKSVPCQGVNVVDVNL 382

BLAST of Carg19649 vs. TAIR 10
Match: AT1G17150.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 2.0e-67
Identity = 144/387 (37.21%), Postives = 215/387 (55.56%), Query Frame = 0

Query: 46  PLSGRGVFNVNDYGAVADGKTENSLAFMAAWEEACAFKGDSIFLIPEGTFLVAGVTFSGP 105
           P++   VF+V  YGA  DGK +N+ AF  AW++AC + G S   IP GTF + GVTF GP
Sbjct: 26  PITRPKVFDVRSYGAKGDGKMDNTNAFTNAWKDACRWNGPSKMYIPLGTFYLGGVTFVGP 85

Query: 106 CFNNLSPKVLIFATLIAPTK---LTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFG 165
           C   +S   +I  TL+AP     +  ++WI+F  + +L + G DG+  L+GQG ++WS  
Sbjct: 86  CDGKIS--FVIDGTLLAPPNNDDIKKEIWINFRYINYLTVFG-DGT--LDGQGKKSWSL- 145

Query: 166 SACRRRMTCPIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNS 225
             C++   CP    ++    V ++ +  I+ ++SK  H      DH  +   NI AP NS
Sbjct: 146 IDCQKDNNCPKLAINMGFDFVKNSSMNGITSLNSKAGHFNFLSVDHFSITRVNIIAPSNS 205

Query: 226 QNTDGIHISNSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKN 285
            NTDGI I+ S+N+ ++N+HI  GDDC+++  G+ +  + N++CGPGHGIS+GSLG+ K+
Sbjct: 206 PNTDGIKIALSSNMQISNTHISTGDDCIAMLSGNTNFDIYNVKCGPGHGISIGSLGKNKD 265

Query: 286 EMDVIGIRVENCTISDTQNGVRVKTWPGNYSS-NAAHLTFRNIVMNNVSNPIIIDQRYCP 345
           E +V G+ V N   + T NG+R+KTW  + S+    +L + N+ M NV NPI IDQ+YCP
Sbjct: 266 EKNVNGLMVRNSVFTGTTNGIRIKTWESSASTIRIINLVYENLQMINVENPIGIDQKYCP 325

Query: 346 NHSCDMKELDIGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTY 405
              C             N G                       S +++  + ++NI GT 
Sbjct: 326 YPPC------------SNMG----------------------DSHIQIRNVTLKNIWGTS 372

Query: 406 NTKLAVNIDCSSTMPCNNIHLSYINLT 429
             K+AV   CS T PC ++ L  INLT
Sbjct: 386 KNKVAVKFQCSKTFPCKDVQLIDINLT 372

BLAST of Carg19649 vs. TAIR 10
Match: AT2G26620.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 254.2 bits (648), Expect = 2.0e-67
Identity = 140/381 (36.75%), Postives = 211/381 (55.38%), Query Frame = 0

Query: 52  VFNVNDYGAVADGKTENSLAFMAAWEEAC-AFKGDSIFLIPEGTFLVAGVTFSGPCFNNL 111
           VFNV  +G+  DGKT+N+ AF + W  AC    G S   +P+GTF + GV F GPC N +
Sbjct: 30  VFNVQRHGSKPDGKTDNTNAFTSIWSRACKRISGSSKIYVPKGTFYLGGVEFVGPCKNPI 89

Query: 112 SPKVLIFATLIAPTK---LTTDVWIHFHSLRHLLLTGLDGSAVLNGQGAQTWSFGSACRR 171
             + +I  TL+AP     +  D WI+F  + +L    + GS  L+GQG Q+W     C +
Sbjct: 90  --EFIIDGTLLAPANPNDIKQDTWINFRYINNL---SISGSGTLDGQGKQSWPLND-CHK 149

Query: 172 RMTCPIFTTSLKLTDVSHAIVKDISLVDSKGFHVAMHGCDHIHLYGFNITAPWNSQNTDG 231
              CP    S+    V+++ +KDI+ ++SK  H       H ++ G  ITAP +S NTDG
Sbjct: 150 NPNCPKLAISMGFAFVNNSNIKDITSLNSKMGHFNFFFVHHFNITGVTITAPSDSPNTDG 209

Query: 232 IHISNSTNINVANSHIGVGDDCVSIGPGSFDILVSNIRCGPGHGISVGSLGRYKNEMDVI 291
           I + + +NI ++N++IG GDDC++I  G+  + +SNI CGPGHGISVGSLG+ K+E DV 
Sbjct: 210 IKMGSCSNIQISNTNIGTGDDCIAILSGTTKLNISNINCGPGHGISVGSLGKNKDEKDVK 269

Query: 292 GIRVENCTISDTQNGVRVKTWPGNYSS-NAAHLTFRNIVMNNVSNPIIIDQRYCPNHSCD 351
            + V +   + T +G+R+KTW  + S    ++  + NI M +V  PI IDQ+YCP+  C+
Sbjct: 270 DLFVRDVIFNGTSDGIRIKTWESSASKILVSNFVYENIQMIDVGKPINIDQKYCPHPPCE 329

Query: 352 MKELDIGRCVSENAGPKEDGLRDPTLVEEGNETFLIRSSSVKLSEMNMRNISGTYNTKLA 411
            +                                  R S V++ ++ ++NI GT   K+A
Sbjct: 330 HE----------------------------------RKSHVQIQDLKLKNIYGTSKNKVA 370

Query: 412 VNIDCSSTMPCNNIHLSYINL 428
           VN+ CS + PC N+ L  IN+
Sbjct: 390 VNLQCSKSFPCKNVELIDINI 370

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7018595.16.0e-268100.00Exopolygalacturonase-like 6, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_022956245.17.7e-19989.61exopolygalacturonase-like [Cucurbita moschata][more]
XP_023539060.11.4e-16364.01exopolygalacturonase-like [Cucurbita pepo subsp. pepo][more]
KAG7028995.13.1e-16364.01Exopolygalacturonase-like 8, partial [Cucurbita argyrosperma subsp. argyrosperma... [more]
XP_038885913.11.9e-16063.50exopolygalacturonase-like [Benincasa hispida][more]
Match NameE-valueIdentityDescription
Q6H9K03.4e-7239.16Exopolygalacturonase (Fragment) OS=Platanus acerifolia OX=140101 GN=plaa2 PE=1 S... [more]
P245485.4e-7039.23Exopolygalacturonase (Fragment) OS=Oenothera organensis OX=3945 PE=2 SV=1[more]
P490621.2e-6637.14Exopolygalacturonase clone GBGE184 OS=Arabidopsis thaliana OX=3702 GN=PGA3 PE=2 ... [more]
Q397661.1e-6536.44Polygalacturonase OS=Gossypium barbadense OX=3634 GN=G9 PE=2 SV=1[more]
P490631.8e-6537.32Exopolygalacturonase clone GBGA483 OS=Arabidopsis thaliana OX=3702 GN=At3g07850 ... [more]
Match NameE-valueIdentityDescription
A0A6J1GXB03.7e-19989.61exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111457998 PE=3 SV=... [more]
A0A6J1ITM71.8e-15695.70exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111479249 PE=3 SV=1[more]
A0A6J1FMM11.7e-14365.28exopolygalacturonase-like OS=Cucurbita moschata OX=3662 GN=LOC111446788 PE=3 SV=... [more]
A0A0A0L9324.2e-14259.70Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G164560 PE=3 SV=1[more]
A0A6J1I8559.3e-14264.77exopolygalacturonase-like OS=Cucurbita maxima OX=3661 GN=LOC111470865 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
AT4G18180.15.3e-7340.96Pectin lyase-like superfamily protein [more]
AT3G14040.11.0e-6837.80Pectin lyase-like superfamily protein [more]
AT1G02790.18.8e-6837.14polygalacturonase 4 [more]
AT1G17150.12.0e-6737.21Pectin lyase-like superfamily protein [more]
AT2G26620.12.0e-6736.75Pectin lyase-like superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR006626Parallel beta-helix repeatSMARTSM00710pbh1coord: 233..254
e-value: 3200.0
score: 3.6
coord: 206..232
e-value: 390.0
score: 9.8
coord: 316..337
e-value: 2900.0
score: 3.9
coord: 256..276
e-value: 3700.0
score: 3.2
coord: 286..307
e-value: 0.51
score: 19.4
IPR000743Glycoside hydrolase, family 28PFAMPF00295Glyco_hydro_28coord: 84..348
e-value: 2.0E-66
score: 224.3
coord: 384..430
e-value: 1.4E-5
score: 24.4
IPR000743Glycoside hydrolase, family 28PROSITEPS00502POLYGALACTURONASEcoord: 263..276
IPR012334Pectin lyase foldGENE3D2.160.20.10coord: 47..450
e-value: 2.4E-97
score: 328.5
NoneNo IPR availablePANTHERPTHR31375:SF33EXOPOLYGALACTURONASE-LIKEcoord: 40..428
NoneNo IPR availablePANTHERPTHR31375FAMILY NOT NAMEDcoord: 40..428
IPR011050Pectin lyase fold/virulence factorSUPERFAMILY51126Pectin lyase-likecoord: 47..435

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19649-RACarg19649-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
molecular_function GO:0004650 polygalacturonase activity