Carg19489 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19489
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	short-chain dehydrogenase TIC 32, chloroplastic-like
Location	Carg_Chr16: 9820200 .. 9821713 (-)
RNA-Seq Expression	Carg19489
Synteny	Carg19489

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGTTGTTTTTCCGAATTTTGATTCTGATTCCATTACATTTCATTAGATTCTCACTCGCTAACGAATTTTGGAGCAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATGTAATTCACTCAAACACGTCGCTAATTCACTCTGTTGTATCGAATGATCACACGATATTTAATTTACCGAAGAAACTAAAACTCAGTTTCGTTCTAATTTTCAGAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGCAAGAACATTACAGAACAATTTTATTTTCATCTCCTTTAATCACGAGGCCTTTTACCATTATTGACGAACCGATGATGCAGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGGTATGAATAATGATTAATTTCCCTTCGCTTATGCATTACTGGCGGTTTGATCATAGCGACAGTGATATTCACTTGCAGCAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGTTCTTCAATACATCACATTCATTGGCATTTTAATTGAATGATTTCACGTCCGCGTTGGATTTTGACACAATCGAAACAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGGTACGAACTACCGAACAATTAAGCTCCTGCATGGAAACTAATCGATCTAAAAAGCGTATACAGATAATTAATTCAATGAGAATGCAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

mRNA sequence

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Coding sequence (CDS)

ATGCTTGAAATGTTCAAGTACCTCGTCGGTTCCGCTGGAGCAAGCGGCTACGGCTCTAAATCCACCGCCGAAGAAGTCAGTGAATCTTGTATGAACTCCATCACCGCCATAGTCACAGGAGCGACGTCGGGAATCGGAGAGGAGACGGCGAGAGTCTTGGCCAAGCGCGGTGTAAGGATCATCATTCCGGCTCGAAATCTAAAAGCCGCTGAGGAAGCGAAGGCGAGAATCGTATCCGAATCGGATTGTTTTGATCATTCACGAATCACGGTTATGGCTCTCGATCTTAGCTCTGTGAATTCCGTCGTGAACTTCGTCTCCAATTTCGAGTCGTTAAATTTGCCTCTTAATCTTCTCATAAACAACGCAGGCAGGTTCTGTTACGAACATGCGTTTTCTGAAGACGGAATTGAGATGACTTTCGCTACTAATTATCTTGGTCATTTTCTCTTGACGAAACTTCTATTGAATAGGATGATAGAAACCGCCAAATCGACAGGAGTCCAAGGCCGTATAGTGAATGTGACGTCCAACATCCATAGCTGGTTTTCGGGCGACATATTCGAATATCTCGGCCAGATAAGCCAGAAAAACAGAGTGTACGATTCGACACGTGCTTACGCGTTCTCCAAACTCGCAAATGTCTTGCACACTGTTCAACTCGCTCACAAACTCCAGGAAATGAAGGCGAATGTTACGGTGAACTGTGTTCATCCAGGCGTTGTGAAAACCAATTTGAACAGAGAACGCGAAGGCTTCGTCACAGATTTGATTTTCTACATGGCGTCCAAATCAAGGTTGTTGAAGACAATTCCTGAAGGTGCAGCAACAAGTTGTTACGTAGCCACGAATCGGAACGTGGAAAATGTGAATGGAAATTACTTCGCGGATTGCAACGATCAAGGAAGTTGCGAATGCAAAACGAAATCCGCGCATTTGGCTGAAGGCCTATGGTCCGCCTCCGAAACCATCGTCTCCACACTTACCGGTAAGCCTCAACCCTAA

Protein sequence

MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP

Homology

[Close]

BLAST of Carg19489 vs. NCBI nr
Match: XP_022923251.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita moschata] >KAG7015802.1 Short-chain dehydrogenase TIC 32, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 654.8 bits (1688), Expect = 3.9e-184
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

[Close]

BLAST of Carg19489 vs. NCBI nr
Match: XP_023552712.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 652.1 bits (1681), Expect = 2.5e-183
Identity = 332/334 (99.40%), Postives = 333/334 (99.70%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYM SKSRLLKTIPEGAAT+CYVATNRNVENVNGNYFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

[Close]

BLAST of Carg19489 vs. NCBI nr
Match: XP_022923250.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita moschata] >KAG6577763.1 Short-chain dehydrogenase TIC 32 A, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 650.2 bits (1676), Expect = 9.7e-183
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

[Close]

BLAST of Carg19489 vs. NCBI nr
Match: XP_023552711.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 647.5 bits (1669), Expect = 6.3e-182
Identity = 332/335 (99.10%), Postives = 333/335 (99.40%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYM SKSRLLKTIPEGAAT+CYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMTSKSRLLKTIPEGAATTCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

[Close]

BLAST of Carg19489 vs. NCBI nr
Match: XP_022965266.1 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 640.6 bits (1651), Expect = 7.7e-180
Identity = 328/334 (98.20%), Postives = 329/334 (98.50%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

[Close]

BLAST of Carg19489 vs. ExPASy Swiss-Prot
Match: A0A078IS66 (Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=TIC32A PE=2 SV=1)

HSP 1 Score: 254.6 bits (649), Expect = 1.5e-66
Identity = 149/330 (45.15%), Postives = 209/330 (63.33%), Query Frame = 0


Query: 2   LEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRI 61
           +E++  + G AG SGYGS STAE+V+ S     +TAI+TG TSGIG E ARVL  RG  +
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 62  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 121
           II +RN KAA ++K  I+     + ++RI  + LDLSS+ SV +F+  F +LN+PLN+LI
Sbjct: 61  IIASRNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILI 120

Query: 122 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 181
           NNAG        SEDGIE  FATN++GHFLLT LLL++M  +A+ +G++GRIVN++S  H
Sbjct: 121 NNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAH 180

Query: 182 --SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 241
             ++  G +F+Y+    +    Y   +AY  SKLAN+LH+  L+ KLQE   N+T+N VH
Sbjct: 181 TYTYTEGIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVH 240

Query: 242 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 301
           PG++ TNL R        +    A    L K IP+GAAT+CYVA + ++++V G YFADC
Sbjct: 241 PGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKDVTGKYFADC 300

Query: 302 NDQGSCECKTKSAHLAEGLWSASETIVSTL 329
           N          +  LA+ LW  S  +V +L
Sbjct: 301 NVTTPSNFAIDTT-LADKLWDFSIKLVESL 319

[Close]

BLAST of Carg19489 vs. ExPASy Swiss-Prot
Match: A0A078ISJ6 (Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=TIC32B PE=3 SV=1)

HSP 1 Score: 253.8 bits (647), Expect = 2.6e-66
Identity = 149/330 (45.15%), Postives = 207/330 (62.73%), Query Frame = 0


Query: 2   LEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRI 61
           +E++  + G AG SGYGS STAE+V+ S     +TAI+TG TSGIG E ARVL  RG  +
Sbjct: 1   MEIYGMVTGKAGKSGYGSASTAEDVTHSIDAKHLTAIITGGTSGIGLEAARVLGMRGAHV 60

Query: 62  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 121
           II ARN KAA ++K  I+     + ++RI  + LDLSS+ SV +F+  F +LN+PLN+LI
Sbjct: 61  IIAARNTKAANDSKEMIL---QMYPNARIDCLQLDLSSIKSVRSFIHQFLALNVPLNILI 120

Query: 122 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 181
           NNAG        SEDGIE  FATN++GHFLLT LLL++M  +A+ +G++GRIVN++S  H
Sbjct: 121 NNAGVMFCPFQLSEDGIESQFATNHIGHFLLTNLLLDKMKSSARESGIEGRIVNLSSIAH 180

Query: 182 --SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVH 241
             ++  G +F+Y+    +    Y   +AY  SKLAN+LH+  L+ KLQE   N+T+N VH
Sbjct: 181 TYTYTEGIMFDYINDPDR----YSEKKAYGQSKLANLLHSNALSRKLQEEGVNITINSVH 240

Query: 242 PGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADC 301
           PG++ TNL R        +    A    L K IP+GAAT+CYVA + +++ V G YF DC
Sbjct: 241 PGLITTNLFRHSG---LGMAVLKAMSFFLWKNIPQGAATTCYVALHPDLKGVTGKYFTDC 300

Query: 302 NDQGSCECKTKSAHLAEGLWSASETIVSTL 329
           N          +  LA+ LW  S  +V +L
Sbjct: 301 NVTTPSNFAIDTT-LADKLWDFSIKLVDSL 319

[Close]

BLAST of Carg19489 vs. ExPASy Swiss-Prot
Match: A2RVM0 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=TIC32 PE=2 SV=1)

HSP 1 Score: 235.0 bits (598), Expect = 1.3e-60
Identity = 144/328 (43.90%), Postives = 199/328 (60.67%), Query Frame = 0


Query: 7   YLVGSAGASGYGSKSTAEEVSESCMNS-ITAIVTGATSGIGEETARVLAKRGVRIIIPAR 66
           +  GS GASG+ S+STAEEV+     + +TAIVTGA+SGIG ETARVL+ RGV +++  R
Sbjct: 2   WFFGSKGASGFSSRSTAEEVTHGVDGTGLTAIVTGASSGIGVETARVLSLRGVHVVMAVR 61

Query: 67  NLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGR 126
           N  +  + K  IV +      +++ VM LDLSS+ SV  F S ++S  LPLNLLINNAG 
Sbjct: 62  NTDSGAKVKEDIVKQ---VPGAKLDVMELDLSSMQSVRKFASEYKSTGLPLNLLINNAGI 121

Query: 127 FCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH--SWF 186
                  S+D IE+ FATN+LGHFLLTKLLL+ M  T++ +  +GRIVN++S  H  S+ 
Sbjct: 122 MACPFMLSKDNIELQFATNHLGHFLLTKLLLDTMKSTSRESKREGRIVNLSSEAHRFSYP 181

Query: 187 SGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVK 246
            G  F+ +   S     Y S RAY  SKL NVLH  +L  +L+E   N+T N +HPG + 
Sbjct: 182 EGVRFDKINDKSS----YSSMRAYGQSKLCNVLHANELTKQLKEDGVNITANSLHPGAIM 241

Query: 247 TNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGS 306
           TNL R    ++   +  +A    +LK++P+GAAT+CYVA N  V  V+G YF D N    
Sbjct: 242 TNLGRYFNPYLAVAVGAVA--KYILKSVPQGAATTCYVALNPQVAGVSGEYFQDSNIAKP 301

Query: 307 CECKTKSAHLAEGLWSASETIVSTLTGK 332
                K   LA+ +W  S  +  + +G+
Sbjct: 302 LPL-VKDTELAKKVWDFSTKLTDSQSGE 319

[Close]

BLAST of Carg19489 vs. ExPASy Swiss-Prot
Match: Q6RVV4 (Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC32 PE=1 SV=1)

HSP 1 Score: 216.9 bits (551), Expect = 3.6e-55
Identity = 137/316 (43.35%), Postives = 187/316 (59.18%), Query Frame = 0


Query: 13  GASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAE 72
           G SG+   STAE+V+       +TAIVTGA+SGIG ET RVLA RG  +I+  RN+ AA+
Sbjct: 9   GVSGFSGSSTAEQVTHGIDATGLTAIVTGASSGIGAETTRVLALRGAHVIMGVRNMVAAK 68

Query: 73  EAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHA 132
           + K  I+ +      +++  + LDLSS++SV  F S F S   PLN+LINNAG       
Sbjct: 69  DVKDTILKD---IPSAKVDAIELDLSSLDSVKKFASEFNSSGRPLNILINNAGIMACPFK 128

Query: 133 FSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH--SWFSGDIFE 192
            S+D IE+ FATN++GHFLLT LLL+ M +T + +  +GRIVNV S  H  ++  G  F+
Sbjct: 129 LSKDNIELQFATNHIGHFLLTNLLLDTMKKTTRESKKEGRIVNVASEAHRFAYPEGIRFD 188

Query: 193 YLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE 252
            +   S     Y++ RAY  SKLANVLH  QL   L+E   N+T N +HPG + TNL R 
Sbjct: 189 KINDQSS----YNNWRAYGQSKLANVLHANQLTKHLKEDGVNITANSLHPGTIVTNLFRH 248

Query: 253 REGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTK 312
               V  LI  +     +LK + +GAAT+CYVA +  V+ V+G YF+D N   +     K
Sbjct: 249 NSA-VNGLINVIG--KLVLKNVQQGAATTCYVALHPQVKGVSGEYFSDSNVYKTTP-HGK 308

Query: 313 SAHLAEGLWSASETIV 326
              LA+ LW  S  +V
Sbjct: 309 DVDLAKKLWDFSINLV 313

[Close]

BLAST of Carg19489 vs. ExPASy Swiss-Prot
Match: Q9HBH5 (Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 5.2e-38
Identity = 118/310 (38.06%), Postives = 168/310 (54.19%), Query Frame = 0


Query: 31  MNSITAIVTGATSGIGEETARVLAKRGVRIIIPARNLKAAEEAKARIVSE----SDCFDH 90
           M+  T ++TGA SG+G  TA  L + G R+I+  R+   AEEA  ++  E    ++C   
Sbjct: 41  MHGKTVLITGANSGLGRATAAELLRLGARVIMGCRDRARAEEAAGQLRRELRQAAECGPE 100

Query: 91  ------SRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINNAGRFCYEHAFSEDGIEMT 150
                   + V  LDL+S+ SV  F          L++LINNAG F   +  +EDG EM 
Sbjct: 101 PGVSGVGELIVRELDLASLRSVRAFCQEMLQEEPRLDVLINNAGIFQCPYMKTEDGFEMQ 160

Query: 151 FATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSWFSGDI-FEYLGQISQKNR 210
           F  N+LGHFLLT LLL  +  +A S     RIV V+S ++ +  GDI F+ L       +
Sbjct: 161 FGVNHLGHFLLTNLLLGLLKSSAPS-----RIVVVSSKLYKY--GDINFDDL----NSEQ 220

Query: 211 VYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVVKTNLNRE-REGFVTDLI 270
            Y+ +  Y+ SKLAN+L T +LA +L+    NVTVN +HPG+V+TNL R      +   +
Sbjct: 221 SYNKSFCYSRSKLANILFTRELARRLE--GTNVTVNVLHPGIVRTNLGRHIHIPLLVKPL 280

Query: 271 FYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQGSCECKTKSAHLAEGLW 329
           F + S +   KT  EGA TS Y+A++  VE V+G YF DC ++     K     +A  LW
Sbjct: 281 FNLVSWA-FFKTPVEGAQTSIYLASSPEVEGVSGRYFGDCKEEELLP-KAMDESVARKLW 335

[Close]

BLAST of Carg19489 vs. ExPASy TrEMBL
Match: A0A6J1E6B3 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 654.8 bits (1688), Expect = 1.9e-184
Identity = 334/334 (100.00%), Postives = 334/334 (100.00%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

[Close]

BLAST of Carg19489 vs. ExPASy TrEMBL
Match: A0A6J1EB90 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111430995 PE=4 SV=1)

HSP 1 Score: 650.2 bits (1676), Expect = 4.7e-183
Identity = 334/335 (99.70%), Postives = 334/335 (99.70%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

[Close]

BLAST of Carg19489 vs. ExPASy TrEMBL
Match: A0A6J1HL83 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 640.6 bits (1651), Expect = 3.7e-180
Identity = 328/334 (98.20%), Postives = 329/334 (98.50%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240
           SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG
Sbjct: 181 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 240

Query: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 300
           VVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCND
Sbjct: 241 VVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCND 300

Query: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 QGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 334

[Close]

BLAST of Carg19489 vs. ExPASy TrEMBL
Match: A0A6J1HN85 (short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465182 PE=4 SV=1)

HSP 1 Score: 636.0 bits (1639), Expect = 9.1e-179
Identity = 328/335 (97.91%), Postives = 329/335 (98.21%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60
           MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI
Sbjct: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESCMNSITAIVTGATSGIGEETARVLAKRGVRI 60

Query: 61  IIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLI 120
           IIPARNLKAAEEAKARIVSES+CFD SRITVMALDLSSV SVVNFVSNFESLNLPLNLLI
Sbjct: 61  IIPARNLKAAEEAKARIVSESNCFDRSRITVMALDLSSVKSVVNFVSNFESLNLPLNLLI 120

Query: 121 NNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180
           NNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH
Sbjct: 121 NNAGRFCYEHALSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIH 180

Query: 181 SWFSGDIFEYLGQISQKN-RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           SWFSGDIFEYLGQISQKN RVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP
Sbjct: 181 SWFSGDIFEYLGQISQKNSRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVVKTNLNREREGFVTDLIFYMASKSRLLK IPEGAATSCYVATNRNVENVNG YFADCN
Sbjct: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKIIPEGAATSCYVATNRNVENVNGKYFADCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP
Sbjct: 301 DQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335

[Close]

BLAST of Carg19489 vs. ExPASy TrEMBL
Match: A0A1S3BLF4 (short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=LOC103491110 PE=4 SV=1)

HSP 1 Score: 562.4 bits (1448), Expect = 1.3e-156
Identity = 287/340 (84.41%), Postives = 312/340 (91.76%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC--MNSITAIVTGATSGIGEETARVLAKRGV 60
           MLEM KYLVGSAGASGYGS STAEEV++S   +NSITAIVTGATSGIGEETARVLAKRG 
Sbjct: 1   MLEMLKYLVGSAGASGYGSNSTAEEVTQSSTHLNSITAIVTGATSGIGEETARVLAKRGA 60

Query: 61  RIIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNL 120
           RI+IPARNLKAAEEAKARIVSE +CFD+SRITVM LDLSS+NSV+NFVS FE+LNLPLNL
Sbjct: 61  RIVIPARNLKAAEEAKARIVSELECFDYSRITVMVLDLSSLNSVMNFVSEFEALNLPLNL 120

Query: 121 LINNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSN 180
           LINNAGRFCYEHA SEDGIEMTFATNYLGHFLLTKLLLN+MIETAKSTG+QGRIVNVTSN
Sbjct: 121 LINNAGRFCYEHATSEDGIEMTFATNYLGHFLLTKLLLNKMIETAKSTGIQGRIVNVTSN 180

Query: 181 IHSWFSGDIFEYLGQISQ----KNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTV 240
           IHSWFSGDIFEYL QIS+     NR YD+TRAY+FSKLAN+LHTVQLAH+LQEMKANVTV
Sbjct: 181 IHSWFSGDIFEYLSQISRNYNNNNREYDATRAYSFSKLANLLHTVQLAHRLQEMKANVTV 240

Query: 241 NCVHPGVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNY 300
           NCVHPGVV+TNLNR+REGF+ DLIF+MASKS+LLKTIP+ AATSCYVAT+R VENVNG Y
Sbjct: 241 NCVHPGVVRTNLNRDREGFLKDLIFFMASKSKLLKTIPQSAATSCYVATHRKVENVNGKY 300

Query: 301 FADCNDQGSCECKTKSAHLAEGLWSASETIVSTLTGKPQP 335
           F DCN+QGSCE  TKSAHLAE LWSASE I+S LTG PQP
Sbjct: 301 FEDCNEQGSCESNTKSAHLAERLWSASEIIISNLTGNPQP 340

[Close]

BLAST of Carg19489 vs. TAIR 10
Match: AT4G24050.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 421.4 bits (1082), Expect = 6.8e-118
Identity = 221/327 (67.58%), Postives = 267/327 (81.65%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  KYL+G+AGASG+GSKSTAEEV+E+C + SITA++TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETGKYLLGAAGASGFGSKSTAEEVTENCDLRSITAVITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL 120
           +I PARN+KAAEEAK RIVSE   F  + I VM LDLSS+ SV NFV++FESL+LPLNLL
Sbjct: 61  LIFPARNVKAAEEAKERIVSE---FPETEIVVMKLDLSSIASVRNFVADFESLDLPLNLL 120

Query: 121 INNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNI 180
           INNAG+  +EHA SEDGIEMTFATNYLGHFLLT LLLN+MI+TA+ TGVQGRIVNVTS I
Sbjct: 121 INNAGKLAHEHAISEDGIEMTFATNYLGHFLLTNLLLNKMIQTAEETGVQGRIVNVTSGI 180

Query: 181 HSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           H WFSGD+ EYL  ISQ    +D+TRAYA SKLANVLHT +L+ +LQ++ ANVTVNCVHP
Sbjct: 181 HGWFSGDLIEYLRLISQPKCQFDATRAYALSKLANVLHTKELSSRLQKIGANVTVNCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           GVV+T L R+REG +TDL+F++ASK  L+KT+P+ AAT+CYVATN  + NV+G YF DCN
Sbjct: 241 GVVRTRLTRDREGLLTDLVFFLASK--LVKTVPQAAATTCYVATNPRLVNVSGKYFTDCN 300

Query: 301 DQGSCECKTKSAHLAEGLWSASETIVS 327
           +       T S+  A  LW+ASE +V+
Sbjct: 301 ETTPSGLGTNSSE-ATKLWAASEILVT 321

[Close]

BLAST of Carg19489 vs. TAIR 10
Match: AT1G64590.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 405.6 bits (1041), Expect = 3.9e-113
Identity = 211/332 (63.55%), Postives = 262/332 (78.92%), Query Frame = 0


Query: 1   MLEMFKYLVGSAGASGYGSKSTAEEVS-ESCMNSITAIVTGATSGIGEETARVLAKRGVR 60
           M+E  K+L+GS G SG+GS+STA+ V+  S + S+TAI+TGATSGIG ETARVLAKRG R
Sbjct: 1   MIETVKHLIGSGGPSGFGSRSTADHVTCNSDLRSLTAIITGATSGIGAETARVLAKRGAR 60

Query: 61  IIIPARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLL 120
           +++PAR++K AEE KARI+SE   F  + I VM LDLSS+ SV  FV +FESLNLPLN+L
Sbjct: 61  LVLPARSVKTAEETKARILSE---FPDAEIIVMHLDLSSLTSVRRFVDDFESLNLPLNIL 120

Query: 121 INNAGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNI 180
           INNAG++ ++HA SEDG+EMTFATNYLGHFLLTKLLL +MIETA  TGVQGRIVNVTS +
Sbjct: 121 INNAGKYAHKHALSEDGVEMTFATNYLGHFLLTKLLLKKMIETAAQTGVQGRIVNVTSVV 180

Query: 181 HSWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHP 240
           HSWFSGD+ +YL  IS+ NR YD+TRAYA SKLANVLHTV+L+  L +M ANVT NCVHP
Sbjct: 181 HSWFSGDMLQYLADISRNNRNYDATRAYALSKLANVLHTVELSRLLHKMDANVTANCVHP 240

Query: 241 GVVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCN 300
           G+VKT L R+REG VTDL+F++ SK  LLK++P+ AAT+CYVAT+  + NV G YF+DCN
Sbjct: 241 GIVKTRLTRDREGVVTDLVFFLTSK--LLKSVPQAAATTCYVATSPRLRNVCGKYFSDCN 300

Query: 301 D-----QGSCECKTKSAHLAEGLWSASETIVS 327
           +      GSC  K      A+ LW+AS+ +VS
Sbjct: 301 EARSSKSGSCNLK------AQRLWTASDLLVS 321

[Close]

BLAST of Carg19489 vs. TAIR 10
Match: AT5G50130.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 289.3 bits (739), Expect = 4.0e-78
Identity = 156/323 (48.30%), Postives = 225/323 (69.66%), Query Frame = 0


Query: 6   KYLVGSAGASGYGSKSTAEEVSESCM---NSITAIVTGATSGIGEETARVLAKRGVRIII 65
           +YL G AG +G+GS+STAE+V++      + +TAI+TG TSGIG ETARVLAKRGVR+++
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVVM 65

Query: 66  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINN 125
             R++K AE  K RI+ E+   D   I +  +DLSS++SV  F S F S +LPLN+LINN
Sbjct: 66  AVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 125

Query: 126 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSW 185
           AG F     FSE+ IE+TFATN+LGH+LLT++L+ +MI+TA+ +G++GRI+N++S IH+W
Sbjct: 126 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 185

Query: 186 FSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVV 245
              D F +   +   +R Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHPG+V
Sbjct: 186 VKPDCFSFPKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGIV 245

Query: 246 KTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCNDQG 305
           KT + R  +G  TD +F +ASK  LLK+I +GAAT+CYVA +   + ++G YFADCN+  
Sbjct: 246 KTGIIRAHKGLFTDSLFLIASK--LLKSISQGAATTCYVALSNETKGLSGKYFADCNET- 305

Query: 306 SCECKTKSAHLAEGLWSASETIV 326
           +C       ++A  L + S  ++
Sbjct: 306 NCSDLANDEYVALKLCTQSRALI 321

[Close]

BLAST of Carg19489 vs. TAIR 10
Match: AT5G02540.1 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 265.4 bits (677), Expect = 6.2e-71
Identity = 152/331 (45.92%), Postives = 218/331 (65.86%), Query Frame = 0


Query: 4   MFKYLVGSAGASGYGSKSTAEEVSESC-MNSITAIVTGATSGIGEETARVLAKRGVRIII 63
           ++  + G  G SG+GS STAEEV++     ++TAI+TG T GIG ETARVL+KRG  ++I
Sbjct: 3   LYSLITGRRGPSGFGSASTAEEVTQGIDATNLTAIITGGTGGIGMETARVLSKRGAHVVI 62

Query: 64  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINN 123
            ARN+ AAE AK  I+ ++    ++R+T++ LDLSS+ S+  FV  F +L+LPLNLLINN
Sbjct: 63  GARNMGAAENAKTEILRQN---ANARVTLLQLDLSSIKSIKAFVREFHALHLPLNLLINN 122

Query: 124 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTS--NIH 183
           AG     +  SEDGIE+ FATN++GHFLLT LLL+ M  TAK++GV+GRI+NV+S  +I+
Sbjct: 123 AGVMFCPYQLSEDGIELQFATNHIGHFLLTNLLLDTMKNTAKTSGVEGRILNVSSVAHIY 182

Query: 184 SWFSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPG 243
           ++  G  F+ +  I      Y   RAY  SKLAN+LH  +L+ +LQE   N+T N VHPG
Sbjct: 183 TYQEGIQFDSINDICS----YSDKRAYGQSKLANILHANELSRQLQEEGVNITANSVHPG 242

Query: 244 VVKTNLNREREGFVTDLIFYMASKSRLLKTIPEGAATSCYVATNRNVENVNGNYFADCND 303
           ++ TNL +     +  L F+      L K IP+GAAT+CYVA + +V+ V G YFADCN+
Sbjct: 243 LILTNLFQHTALLMRFLKFF---SFYLWKNIPQGAATTCYVALHPSVKGVTGKYFADCNE 302

Query: 304 QGSCECKTKSAHLAEGLWSASETIVSTLTGK 332
               +   +   LA+ LW  S  ++++++ K
Sbjct: 303 VTPSKL-ARDETLAQKLWDFSVKLINSVSKK 322

[Close]

BLAST of Carg19489 vs. TAIR 10
Match: AT5G50130.2 (NAD(P)-binding Rossmann-fold superfamily protein )

HSP 1 Score: 253.4 bits (646), Expect = 2.5e-67
Identity = 137/271 (50.55%), Postives = 194/271 (71.59%), Query Frame = 0


Query: 6   KYLVGSAGASGYGSKSTAEEVSESCM---NSITAIVTGATSGIGEETARVLAKRGVRIII 65
           +YL G AG +G+GS+STAE+V++      + +TAI+TG TSGIG ETARVLAKRGVR+++
Sbjct: 6   RYLAGIAGPNGFGSRSTAEQVTQHSFFPCSHLTAIITGGTSGIGAETARVLAKRGVRVVM 65

Query: 66  PARNLKAAEEAKARIVSESDCFDHSRITVMALDLSSVNSVVNFVSNFESLNLPLNLLINN 125
             R++K AE  K RI+ E+   D   I +  +DLSS++SV  F S F S +LPLN+LINN
Sbjct: 66  AVRDMKKAEMVKERIIRENPEAD---IILFEIDLSSLSSVARFCSQFLSQDLPLNILINN 125

Query: 126 AGRFCYEHAFSEDGIEMTFATNYLGHFLLTKLLLNRMIETAKSTGVQGRIVNVTSNIHSW 185
           AG F     FSE+ IE+TFATN+LGH+LLT++L+ +MI+TA+ +G++GRI+N++S IH+W
Sbjct: 126 AGVFSPNLEFSEEKIELTFATNFLGHYLLTEMLIEKMIDTAEKSGIEGRIINLSSVIHNW 185

Query: 186 FSGDIFEYLGQISQKNRVYDSTRAYAFSKLANVLHTVQLAHKLQEMKANVTVNCVHPGVV 245
              D F +   +   +R Y+ TRAYA SKLA +LH   L+ +L++  ANVT+N VHPG+V
Sbjct: 186 VKPDCFSFPKLLHPISR-YNGTRAYAQSKLATILHAKALSKQLKDRNANVTINAVHPGIV 245

Query: 246 KTNLNREREGFVTDLIFYMASKSRLLKTIPE 274
           KT + R  +G  TD +F +ASK  LLK+I +
Sbjct: 246 KTGIIRAHKGLFTDSLFLIASK--LLKSISQ 270

The following BLAST results are available for this feature:

BLAST of Carg19489 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022923251.1	3.9e-184	100.00	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita mosch...	[more]
XP_023552712.1	2.5e-183	99.40	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita pepo ...	[more]
XP_022923250.1	9.7e-183	99.70	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita mosch...	[more]
XP_023552711.1	6.3e-182	99.10	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 [Cucurbita pepo ...	[more]
XP_022965266.1	7.7e-180	98.20	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 [Cucurbita maxim...	[more]

BLAST of Carg19489 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
A0A078IS66	1.5e-66	45.15	Short-chain dehydrogenase TIC 32 A, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A0A078ISJ6	2.6e-66	45.15	Short-chain dehydrogenase TIC 32 B, chloroplastic OS=Brassica napus OX=3708 GN=T...	[more]
A2RVM0	1.3e-60	43.90	Short-chain dehydrogenase TIC 32, chloroplastic OS=Arabidopsis thaliana OX=3702 ...	[more]
Q6RVV4	3.6e-55	43.35	Short-chain dehydrogenase TIC 32, chloroplastic OS=Pisum sativum OX=3888 GN=TIC3...	[more]
Q9HBH5	5.2e-38	38.06	Retinol dehydrogenase 14 OS=Homo sapiens OX=9606 GN=RDH14 PE=1 SV=1	[more]

BLAST of Carg19489 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E6B3	1.9e-184	100.00	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita mos...	[more]
A0A6J1EB90	4.7e-183	99.70	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita mos...	[more]
A0A6J1HL83	3.7e-180	98.20	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X2 OS=Cucurbita max...	[more]
A0A6J1HN85	9.1e-179	97.91	short-chain dehydrogenase TIC 32, chloroplastic-like isoform X1 OS=Cucurbita max...	[more]
A0A1S3BLF4	1.3e-156	84.41	short-chain dehydrogenase TIC 32, chloroplastic-like OS=Cucumis melo OX=3656 GN=...	[more]

BLAST of Carg19489 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G24050.1	6.8e-118	67.58	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT1G64590.1	3.9e-113	63.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.1	4.0e-78	48.30	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G02540.1	6.2e-71	45.92	NAD(P)-binding Rossmann-fold superfamily protein	[more]
AT5G50130.2	2.5e-67	50.55	NAD(P)-binding Rossmann-fold superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR002347	Short-chain dehydrogenase/reductase SDR	PRINTS	PR00081	GDHRDH	coord: 164..180 score: 27.08 coord: 230..247 score: 36.77 coord: 35..52 score: 46.16 coord: 114..125 score: 42.55 coord: 207..226 score: 30.23
IPR002347	Short-chain dehydrogenase/reductase SDR	PFAM	PF00106	adh_short	coord: 35..179 e-value: 4.5E-25 score: 88.3
None	No IPR available	GENE3D	3.40.50.720		coord: 28..328 e-value: 2.4E-69 score: 235.6
None	No IPR available	PANTHER	PTHR24320:SF114	OS06G0129100 PROTEIN	coord: 4..328
None	No IPR available	PANTHER	PTHR24320	RETINOL DEHYDROGENASE	coord: 4..328
IPR036291	NAD(P)-binding domain superfamily	SUPERFAMILY	51735	NAD(P)-binding Rossmann-fold domains	coord: 35..299

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19489-RA	Carg19489-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0016491	oxidoreductase activity