Carg19462 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19462
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	SKP1-like protein
Location	Carg_Chr16: 10012080 .. 10013981 (+)
RNA-Seq Expression	Carg19462
Synteny	Carg19462

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TTCTTCGTCCTCTTCTTCACAAAAATCACCATCAATCTTTCTGCTAACCCAAATCCAAATCCAAATCCAAATCCATGTCCTCCTCCAAGAAGATTACTCTCAAGAGCTCCGATGGCGAGTCTTTTGAAATCGATGAGGTCGTTGCTCTCGAGTCTCAGACCATCCGCCACATGATCGAGGACGATTGCGCCGATAACGGCATCCCTCTTCCCAACGTCACTAGCAAGATCCTGGCCAAGGTCATCGAGTACTGTCACAAGCACGTCGATGCGTCCTCCGCCGATCCCCGCGTGTCTGAAGAGGATCTCAAGGCCTGGGACCGCGATTTCGTTAATGTCGACCAAGCCACTCTTTTCGATCTCATTCTGGTAACCTTTTCTTCCTGATTTAGATCGATCGACTCGATTGTCTAGGGTTTACCTGGTTCCTATGGTTTTTTTTTTTTCAAGAATTTGACTCGGCAAACAATCGCTTTGATCTTCTTTGTCGCTGATTACTGTTCTTCTTTTTCTGATATATCTGTATCGACGTTTGTAATTGTGCTAGGGCTTGTTATTGTTTGATGTTTTGGAAATGGAAAGTTCGTTCAAAGGAAAAGTTCTACTGGAAATTGTTTAGTATTGTTGATCAATCGGTATGGTGCCTAAGTAATGGATTTAGATTCAATCGGACGAGCATGGTCTTTTCACTTTGATGATTGAAGACGGTGATGCAAAATGCTTTCGGCATCATTATCCTTTGTCCTTCATGTTGAGATTGGGGTTAATGGACATCTGTATTTGGCGTCATATTGAAATGATTGTTGCTTAGGTTAATCTTCGTGGTTCTATATTTAAGCCGTGTTCTTGTTGCACAGAATTCCAAGCTGCCTGGGGCTTCATTCTTGACTATTATTATTCTTGATAATTTCTTGATTTGGCGCCATATAAAATTCTCTCCAATATTCTACTCTGCAGGCTGCAAATTATCTGAACATCAAGAGCTTGTTAGACCTGACATGCCAGACCGTAGCTGACATGATCAAGGGTAAGACCCCAGAGGAGATCCGAAAGACCTTTAACATCAAGAACGATTTCACACCTGAGGAAGAGGAGGAAGTTCGCAGGGAGAATCAGTGGGCTTTTGAATGATTTGAAATCTCATAAGAGTGCTCAAGGATCTTGATCAATAAGGTCTGTACATGGTAGTAGTATTCGATCTCTGTTGTATTCACATAGTTTGTGAAAATGGTACTGTATGAACCTAATCATAATTGTATCATGATCATGTTTGTGTGAGGAATGCATTGACTACCTAATTGTCTCATACCATCATTATGTTAAATTTTGATAGTAGCCTATGCTCTAGTTATCTTGTCTCCCCGTAATTGAAGCCTTGTGAGGGAAGGGAGGAAGCCATGTTGCCTACACTCTCTTCTCAATTCTCACTACTCGACTTTGTTTTTGTGTTTCTTGTTGCTTGATGCTTTGTTCCACTTTCGTCGACTTATGTGCTTTGTGTTTGTCATCGGACTTCTTTGTTATGTGTTTGTTGTTGGACTTCTCCGCTCGTGCGCTCAGTTGTCCTTAGACTTACTAGCTTGGTTCAACATTTGTCAGCTTTGGTAATTGGTTAATTGGTTCTGCCTTTGCCCTTGGTTCTTGATGAAACAACCAACCAACCTAACATGAATCATGTATACCCCTAATCCATTTCGTCAATTTTTGACCAATTGCAGTGGAATGCTATAACAACCACAACACAACAATAGAGATGTTACTCTTGGGTTTGAAAATTCGTTGAATTCAACGTGTCTTTTAACACAAAGTTAAGCATACTTGTATGAACATCCAATATCAAGGATTATTTGTGGGACTCATTCCCCCTTTCACAAGTCTAGAATGTATGTCTACACCAATGATT

mRNA sequence

TTCTTCGTCCTCTTCTTCACAAAAATCACCATCAATCTTTCTGCTAACCCAAATCCAAATCCAAATCCAAATCCATGTCCTCCTCCAAGAAGATTACTCTCAAGAGCTCCGATGGCGAGTCTTTTGAAATCGATGAGGTCGTTGCTCTCGAGTCTCAGACCATCCGCCACATGATCGAGGACGATTGCGCCGATAACGGCATCCCTCTTCCCAACGTCACTAGCAAGATCCTGGCCAAGGTCATCGAGTACTGTCACAAGCACGTCGATGCGTCCTCCGCCGATCCCCGCGTGTCTGAAGAGGATCTCAAGGCCTGGGACCGCGATTTCGTTAATGTCGACCAAGCCACTCTTTTCGATCTCATTCTGGCTGCAAATTATCTGAACATCAAGAGCTTGTTAGACCTGACATGCCAGACCGTAGCTGACATGATCAAGGGTAAGACCCCAGAGGAGATCCGAAAGACCTTTAACATCAAGAACGATTTCACACCTGAGGAAGAGGAGGAAGTTCGCAGGGAGAATCAGTGGGCTTTTGAATGATTTGAAATCTCATAAGAGTGCTCAAGGATCTTGATCAATAAGGTCTGTACATGGTAGTAGTATTCGATCTCTGTTGTATTCACATAGTTTGTGAAAATGGTACTGTATGAACCTAATCATAATTGTATCATGATCATGTTTGTGTGAGGAATGCATTGACTACCTAATTGTCTCATACCATCATTATGTTAAATTTTGATAGTAGCCTATGCTCTAGTTATCTTGTCTCCCCGTAATTGAAGCCTTGTGAGGGAAGGGAGGAAGCCATGTTGCCTACACTCTCTTCTCAATTCTCACTACTCGACTTTGTTTTTGTGTTTCTTGTTGCTTGATGCTTTGTTCCACTTTCGTCGACTTATGTGCTTTGTGTTTGTCATCGGACTTCTTTGTTATGTGTTTGTTGTTGGACTTCTCCGCTCGTGCGCTCAGTTGTCCTTAGACTTACTAGCTTGGTTCAACATTTGTCAGCTTTGGTAATTGGTTAATTGGTTCTGCCTTTGCCCTTGGTTCTTGATGAAACAACCAACCAACCTAACATGAATCATGTATACCCCTAATCCATTTCGTCAATTTTTGACCAATTGCAGTGGAATGCTATAACAACCACAACACAACAATAGAGATGTTACTCTTGGGTTTGAAAATTCGTTGAATTCAACGTGTCTTTTAACACAAAGTTAAGCATACTTGTATGAACATCCAATATCAAGGATTATTTGTGGGACTCATTCCCCCTTTCACAAGTCTAGAATGTATGTCTACACCAATGATT

Coding sequence (CDS)

ATGTCCTCCTCCAAGAAGATTACTCTCAAGAGCTCCGATGGCGAGTCTTTTGAAATCGATGAGGTCGTTGCTCTCGAGTCTCAGACCATCCGCCACATGATCGAGGACGATTGCGCCGATAACGGCATCCCTCTTCCCAACGTCACTAGCAAGATCCTGGCCAAGGTCATCGAGTACTGTCACAAGCACGTCGATGCGTCCTCCGCCGATCCCCGCGTGTCTGAAGAGGATCTCAAGGCCTGGGACCGCGATTTCGTTAATGTCGACCAAGCCACTCTTTTCGATCTCATTCTGGCTGCAAATTATCTGAACATCAAGAGCTTGTTAGACCTGACATGCCAGACCGTAGCTGACATGATCAAGGGTAAGACCCCAGAGGAGATCCGAAAGACCTTTAACATCAAGAACGATTTCACACCTGAGGAAGAGGAGGAAGTTCGCAGGGAGAATCAGTGGGCTTTTGAATGA

Protein sequence

MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE

Homology

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BLAST of Carg19462 vs. NCBI nr
Match: XP_022965297.1 (SKP1-like protein 1A [Cucurbita maxima] >KAG6577790.1 SKP1-like protein 1A, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015829.1 SKP1-like protein 1A [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 306.2 bits (783), Expect = 1.6e-79
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC
Sbjct: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
           HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI
Sbjct: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

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BLAST of Carg19462 vs. NCBI nr
Match: XP_022923340.1 (SKP1-like protein 1A [Cucurbita moschata])

HSP 1 Score: 304.7 bits (779), Expect = 4.6e-79
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKIL KVIEYC
Sbjct: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILVKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
           HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI
Sbjct: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

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BLAST of Carg19462 vs. NCBI nr
Match: XP_023553541.1 (SKP1-like protein 1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 302.4 bits (773), Expect = 2.3e-78
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEY 
Sbjct: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYS 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
           HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI
Sbjct: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

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BLAST of Carg19462 vs. NCBI nr
Match: XP_038903121.1 (SKP1-like protein 1A [Benincasa hispida])

HSP 1 Score: 293.5 bits (750), Expect = 1.1e-75
Identity = 146/155 (94.19%), Postives = 152/155 (98.06%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKITLKSSDG+SFE+DE VAL+SQTIRHMIEDDCADNGIPLPNVTS ILAKVIEYC
Sbjct: 1   MSSSKKITLKSSDGDSFEVDEAVALQSQTIRHMIEDDCADNGIPLPNVTSNILAKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
            KHVDASSADPR+S++DLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI
Sbjct: 61  RKHVDASSADPRLSDDDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

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BLAST of Carg19462 vs. NCBI nr
Match: XP_022145436.1 (SKP1-like protein 1A [Momordica charantia])

HSP 1 Score: 288.9 bits (738), Expect = 2.6e-74
Identity = 144/155 (92.90%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKI LKSSDGE FE+DE VALESQTIRHMIEDDCADNGIPLPNV+SKILAKVIEYC
Sbjct: 1   MSSSKKIILKSSDGEPFEVDEAVALESQTIRHMIEDDCADNGIPLPNVSSKILAKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
            KHVDAS+++PR SEEDLKAWDRDFVNVDQATLFDLILAANYLNIK+LLDLTCQTVADMI
Sbjct: 61  RKHVDASNSEPRASEEDLKAWDRDFVNVDQATLFDLILAANYLNIKNLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

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BLAST of Carg19462 vs. ExPASy Swiss-Prot
Match: Q39255 (SKP1-like protein 1A OS=Arabidopsis thaliana OX=3702 GN=SKP1A PE=1 SV=1)

HSP 1 Score: 253.1 bits (645), Expect = 2.1e-66
Identity = 125/159 (78.62%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 3   SSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHK 62
           S+KKI LKSSDGESFE++E VALESQTI HM+EDDC DNG+PLPNVTSKILAKVIEYC +
Sbjct: 2   SAKKIVLKSSDGESFEVEEAVALESQTIAHMVEDDCVDNGVPLPNVTSKILAKVIEYCKR 61

Query: 63  HVDASSADPR------VSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTV 122
           HV+A+++          S++DLKAWD DF+ +DQATLF+LILAANYLNIK+LLDLTCQTV
Sbjct: 62  HVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQATLFELILAANYLNIKNLLDLTCQTV 121

Query: 123 ADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           ADMIKGKTPEEIR TFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 122 ADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160

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BLAST of Carg19462 vs. ExPASy Swiss-Prot
Match: Q9FHW7 (SKP1-like protein 1B OS=Arabidopsis thaliana OX=3702 GN=SKP1B PE=1 SV=1)

HSP 1 Score: 252.7 bits (644), Expect = 2.7e-66
Identity = 131/171 (76.61%), Postives = 143/171 (83.63%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MS+ +KITLKSSDGE+FEIDE VALESQTI+HMIEDDC DNGIPLPNVTSKIL+KVIEYC
Sbjct: 1   MSTVRKITLKSSDGENFEIDEAVALESQTIKHMIEDDCTDNGIPLPNVTSKILSKVIEYC 60

Query: 61  HKHVDA-----SSADPRV-----------SEEDLKAWDRDFVNVDQATLFDLILAANYLN 120
            +HV+A     ++AD              S+EDLK WD +F+ VDQ TLFDLILAANYLN
Sbjct: 61  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQGTLFDLILAANYLN 120

Query: 121 IKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           IK LLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 IKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171

[Close]

BLAST of Carg19462 vs. ExPASy Swiss-Prot
Match: Q6PL11 (SKP1-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP1 PE=1 SV=1)

HSP 1 Score: 251.9 bits (642), Expect = 4.7e-66
Identity = 130/166 (78.31%), Postives = 138/166 (83.13%), Query Frame = 0


Query: 5   KKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHKHV 64
           K ITLKSSDGE FE++E VA+ESQTIRHMIEDDCADNGIPLPNV SKIL+KVIEYC+KHV
Sbjct: 8   KMITLKSSDGEEFEVEEAVAMESQTIRHMIEDDCADNGIPLPNVNSKILSKVIEYCNKHV 67

Query: 65  ---------------DASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLL 124
                           A++A P  S EDLK WD DFV VDQATLFDLILAANYLNIK LL
Sbjct: 68  HAAAAAASKAADDAASAAAAVPPPSGEDLKNWDADFVKVDQATLFDLILAANYLNIKGLL 127

Query: 125 DLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           DLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEE+RRENQWAFE
Sbjct: 128 DLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEIRRENQWAFE 173

[Close]

BLAST of Carg19462 vs. ExPASy Swiss-Prot
Match: Q651E8 (SKP1-like protein 20 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP20 PE=1 SV=1)

HSP 1 Score: 226.5 bits (576), Expect = 2.1e-58
Identity = 118/165 (71.52%), Postives = 135/165 (81.82%), Query Frame = 0


Query: 7   ITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHKHVD- 66
           ITL+S +G+ FE+ E VA+ESQTIRHMIED CAD GIPLPNV++KIL+KVIEYC KHV+ 
Sbjct: 11  ITLRSCEGQVFEVAEAVAMESQTIRHMIEDKCADTGIPLPNVSAKILSKVIEYCSKHVEA 70

Query: 67  ----ASSAD-----------PRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLD 126
               A++AD            +  E++LK +D +FV VDQ+TLFDLILAANYLNIK LLD
Sbjct: 71  RGGAAAAADGDAPAPAAVEANKAVEDELKTFDAEFVKVDQSTLFDLILAANYLNIKGLLD 130

Query: 127 LTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           LTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 131 LTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 175

[Close]

BLAST of Carg19462 vs. ExPASy Swiss-Prot
Match: O49484 (SKP1-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=ASK11 PE=1 SV=1)

HSP 1 Score: 222.6 bits (566), Expect = 3.0e-57
Identity = 112/153 (73.20%), Postives = 130/153 (84.97%), Query Frame = 0


Query: 3   SSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHK 62
           SSK I L SSDG+SFE++E VA++SQTI HM+EDDC  +GIPL NV SKIL KVIEYC K
Sbjct: 2   SSKMIVLMSSDGQSFEVEEAVAIQSQTIAHMVEDDCVADGIPLANVESKILVKVIEYCKK 61

Query: 63  HVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKG 122
           H     A+P +SEEDL  WD  F++++Q+T+F+LILAANYLNIKSLLDLTCQTVADMIKG
Sbjct: 62  H-HVDEANP-ISEEDLNNWDEKFMDLEQSTIFELILAANYLNIKSLLDLTCQTVADMIKG 121

Query: 123 KTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KTPEEIR TFNI+NDFTPEEEE VR+ENQWAFE
Sbjct: 122 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

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BLAST of Carg19462 vs. ExPASy TrEMBL
Match: A0A6J1HJY3 (SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465205 PE=3 SV=1)

HSP 1 Score: 306.2 bits (783), Expect = 7.7e-80
Identity = 155/155 (100.00%), Postives = 155/155 (100.00%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC
Sbjct: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
           HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI
Sbjct: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

[Close]

BLAST of Carg19462 vs. ExPASy TrEMBL
Match: A0A6J1EBI5 (SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111431062 PE=3 SV=1)

HSP 1 Score: 304.7 bits (779), Expect = 2.2e-79
Identity = 154/155 (99.35%), Postives = 154/155 (99.35%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKIL KVIEYC
Sbjct: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILVKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
           HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI
Sbjct: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

[Close]

BLAST of Carg19462 vs. ExPASy TrEMBL
Match: A0A6J1CWB0 (SKP1-like protein OS=Momordica charantia OX=3673 GN=LOC111014883 PE=3 SV=1)

HSP 1 Score: 288.9 bits (738), Expect = 1.3e-74
Identity = 144/155 (92.90%), Postives = 150/155 (96.77%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MSSSKKI LKSSDGE FE+DE VALESQTIRHMIEDDCADNGIPLPNV+SKILAKVIEYC
Sbjct: 1   MSSSKKIILKSSDGEPFEVDEAVALESQTIRHMIEDDCADNGIPLPNVSSKILAKVIEYC 60

Query: 61  HKHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMI 120
            KHVDAS+++PR SEEDLKAWDRDFVNVDQATLFDLILAANYLNIK+LLDLTCQTVADMI
Sbjct: 61  RKHVDASNSEPRASEEDLKAWDRDFVNVDQATLFDLILAANYLNIKNLLDLTCQTVADMI 120

Query: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 KGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 155

[Close]

BLAST of Carg19462 vs. ExPASy TrEMBL
Match: A0A6J5VI66 (SKP1-like protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS45864 PE=3 SV=1)

HSP 1 Score: 282.0 bits (720), Expect = 1.6e-72
Identity = 140/154 (90.91%), Postives = 148/154 (96.10%), Query Frame = 0


Query: 2   SSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCH 61
           SSSKKITLKSSDGESFE++E VALESQTI+HMIEDDCADNGIPLPNVTSKILAKVIEYC 
Sbjct: 3   SSSKKITLKSSDGESFEVEEAVALESQTIKHMIEDDCADNGIPLPNVTSKILAKVIEYCK 62

Query: 62  KHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIK 121
           KHVDA+  D ++SE+DLKAWD+DFV VDQATLFDLILAANYLNIKSLLDLTCQTVADMIK
Sbjct: 63  KHVDAAKPDEKISEDDLKAWDQDFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIK 122

Query: 122 GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 123 GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156

[Close]

BLAST of Carg19462 vs. ExPASy TrEMBL
Match: A0A6P5SPM9 (SKP1-like protein OS=Prunus avium OX=42229 GN=LOC110758167 PE=3 SV=1)

HSP 1 Score: 281.2 bits (718), Expect = 2.6e-72
Identity = 140/154 (90.91%), Postives = 147/154 (95.45%), Query Frame = 0


Query: 2   SSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCH 61
           SSSKKITLKSSDGESFE++E VALESQTI+HMIEDDCADNGIPLPNVTSKILAKVIEYC 
Sbjct: 3   SSSKKITLKSSDGESFEVEEAVALESQTIKHMIEDDCADNGIPLPNVTSKILAKVIEYCK 62

Query: 62  KHVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIK 121
           KHVDA+  D ++SE+DLKAWD DFV VDQATLFDLILAANYLNIKSLLDLTCQTVADMIK
Sbjct: 63  KHVDAAKPDEKISEDDLKAWDTDFVKVDQATLFDLILAANYLNIKSLLDLTCQTVADMIK 122

Query: 122 GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 123 GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156

[Close]

BLAST of Carg19462 vs. TAIR 10
Match: AT1G75950.1 (S phase kinase-associated protein 1 )

HSP 1 Score: 253.1 bits (645), Expect = 1.5e-67
Identity = 125/159 (78.62%), Postives = 141/159 (88.68%), Query Frame = 0


Query: 3   SSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHK 62
           S+KKI LKSSDGESFE++E VALESQTI HM+EDDC DNG+PLPNVTSKILAKVIEYC +
Sbjct: 2   SAKKIVLKSSDGESFEVEEAVALESQTIAHMVEDDCVDNGVPLPNVTSKILAKVIEYCKR 61

Query: 63  HVDASSADPR------VSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTV 122
           HV+A+++          S++DLKAWD DF+ +DQATLF+LILAANYLNIK+LLDLTCQTV
Sbjct: 62  HVEAAASKAEAVEGAATSDDDLKAWDADFMKIDQATLFELILAANYLNIKNLLDLTCQTV 121

Query: 123 ADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           ADMIKGKTPEEIR TFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 122 ADMIKGKTPEEIRTTFNIKNDFTPEEEEEVRRENQWAFE 160

[Close]

BLAST of Carg19462 vs. TAIR 10
Match: AT5G42190.1 (E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein )

HSP 1 Score: 252.7 bits (644), Expect = 1.9e-67
Identity = 131/171 (76.61%), Postives = 143/171 (83.63%), Query Frame = 0


Query: 1   MSSSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYC 60
           MS+ +KITLKSSDGE+FEIDE VALESQTI+HMIEDDC DNGIPLPNVTSKIL+KVIEYC
Sbjct: 1   MSTVRKITLKSSDGENFEIDEAVALESQTIKHMIEDDCTDNGIPLPNVTSKILSKVIEYC 60

Query: 61  HKHVDA-----SSADPRV-----------SEEDLKAWDRDFVNVDQATLFDLILAANYLN 120
            +HV+A     ++AD              S+EDLK WD +F+ VDQ TLFDLILAANYLN
Sbjct: 61  KRHVEAAEKSETTADAAAATTTTTVASGSSDEDLKTWDSEFIKVDQGTLFDLILAANYLN 120

Query: 121 IKSLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           IK LLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE
Sbjct: 121 IKGLLDLTCQTVADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 171

[Close]

BLAST of Carg19462 vs. TAIR 10
Match: AT4G34210.1 (SKP1-like 11 )

HSP 1 Score: 222.6 bits (566), Expect = 2.2e-58
Identity = 112/153 (73.20%), Postives = 130/153 (84.97%), Query Frame = 0


Query: 3   SSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHK 62
           SSK I L SSDG+SFE++E VA++SQTI HM+EDDC  +GIPL NV SKIL KVIEYC K
Sbjct: 2   SSKMIVLMSSDGQSFEVEEAVAIQSQTIAHMVEDDCVADGIPLANVESKILVKVIEYCKK 61

Query: 63  HVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIKG 122
           H     A+P +SEEDL  WD  F++++Q+T+F+LILAANYLNIKSLLDLTCQTVADMIKG
Sbjct: 62  H-HVDEANP-ISEEDLNNWDEKFMDLEQSTIFELILAANYLNIKSLLDLTCQTVADMIKG 121

Query: 123 KTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           KTPEEIR TFNI+NDFTPEEEE VR+ENQWAFE
Sbjct: 122 KTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of Carg19462 vs. TAIR 10
Match: AT4G34470.1 (SKP1-like 12 )

HSP 1 Score: 220.7 bits (561), Expect = 8.2e-58
Identity = 113/154 (73.38%), Postives = 131/154 (85.06%), Query Frame = 0


Query: 3   SSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHK 62
           SSK I L SSDG+SFE++E VA++SQTI HM+EDDC  +GIPL NV SKIL KVIEYC K
Sbjct: 2   SSKMIVLMSSDGQSFEVEEAVAIQSQTIAHMVEDDCVADGIPLANVESKILVKVIEYCKK 61

Query: 63  -HVDASSADPRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQTVADMIK 122
            HVD   A+P +SEEDL  WD  F++++Q+T+F+LILAANYLNIKSL DLTCQTVADMIK
Sbjct: 62  YHVD--EANP-ISEEDLNKWDEKFMDLEQSTIFELILAANYLNIKSLFDLTCQTVADMIK 121

Query: 123 GKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           GKTPEEIR TFNI+NDFTPEEEE VR+ENQWAFE
Sbjct: 122 GKTPEEIRSTFNIENDFTPEEEEAVRKENQWAFE 152

[Close]

BLAST of Carg19462 vs. TAIR 10
Match: AT1G20140.1 (SKP1-like 4 )

HSP 1 Score: 215.7 bits (548), Expect = 2.6e-56
Identity = 109/160 (68.12%), Postives = 127/160 (79.38%), Query Frame = 0


Query: 3   SSKKITLKSSDGESFEIDEVVALESQTIRHMIEDDCADNGIPLPNVTSKILAKVIEYCHK 62
           + K I LKSSDGESFEI+E VA++SQTI+HMIEDDCADNGIPLPNVT  ILAKVIEYC K
Sbjct: 4   TKKMIILKSSDGESFEIEEAVAVKSQTIKHMIEDDCADNGIPLPNVTGAILAKVIEYCKK 63

Query: 63  HVDASSAD-------PRVSEEDLKAWDRDFVNVDQATLFDLILAANYLNIKSLLDLTCQT 122
           HV+A++              ++LK WD +FV VDQ TLFDLILAANYLNI  LLDLTC+ 
Sbjct: 64  HVEAAAEAGGDKDFYGSAENDELKNWDSEFVKVDQPTLFDLILAANYLNIGGLLDLTCKA 123

Query: 123 VADMIKGKTPEEIRKTFNIKNDFTPEEEEEVRRENQWAFE 156
           VAD ++GKTPE++R  FNIKND+TPEEE EVR EN+WAFE
Sbjct: 124 VADQMRGKTPEQMRAHFNIKNDYTPEEEAEVRNENKWAFE 163

The following BLAST results are available for this feature:

BLAST of Carg19462 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022965297.1	1.6e-79	100.00	SKP1-like protein 1A [Cucurbita maxima] >KAG6577790.1 SKP1-like protein 1A, part...	[more]
XP_022923340.1	4.6e-79	99.35	SKP1-like protein 1A [Cucurbita moschata]	[more]
XP_023553541.1	2.3e-78	99.35	SKP1-like protein 1A [Cucurbita pepo subsp. pepo]	[more]
XP_038903121.1	1.1e-75	94.19	SKP1-like protein 1A [Benincasa hispida]	[more]
XP_022145436.1	2.6e-74	92.90	SKP1-like protein 1A [Momordica charantia]	[more]

BLAST of Carg19462 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q39255	2.1e-66	78.62	SKP1-like protein 1A OS=Arabidopsis thaliana OX=3702 GN=SKP1A PE=1 SV=1	[more]
Q9FHW7	2.7e-66	76.61	SKP1-like protein 1B OS=Arabidopsis thaliana OX=3702 GN=SKP1B PE=1 SV=1	[more]
Q6PL11	4.7e-66	78.31	SKP1-like protein 1 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP1 PE=1 SV=1	[more]
Q651E8	2.1e-58	71.52	SKP1-like protein 20 OS=Oryza sativa subsp. japonica OX=39947 GN=SKP20 PE=1 SV=1	[more]
O49484	3.0e-57	73.20	SKP1-like protein 11 OS=Arabidopsis thaliana OX=3702 GN=ASK11 PE=1 SV=1	[more]

BLAST of Carg19462 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HJY3	7.7e-80	100.00	SKP1-like protein OS=Cucurbita maxima OX=3661 GN=LOC111465205 PE=3 SV=1	[more]
A0A6J1EBI5	2.2e-79	99.35	SKP1-like protein OS=Cucurbita moschata OX=3662 GN=LOC111431062 PE=3 SV=1	[more]
A0A6J1CWB0	1.3e-74	92.90	SKP1-like protein OS=Momordica charantia OX=3673 GN=LOC111014883 PE=3 SV=1	[more]
A0A6J5VI66	1.6e-72	90.91	SKP1-like protein OS=Prunus armeniaca OX=36596 GN=CURHAP_LOCUS45864 PE=3 SV=1	[more]
A0A6P5SPM9	2.6e-72	90.91	SKP1-like protein OS=Prunus avium OX=42229 GN=LOC110758167 PE=3 SV=1	[more]

BLAST of Carg19462 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G75950.1	1.5e-67	78.62	S phase kinase-associated protein 1	[more]
AT5G42190.1	1.9e-67	76.61	E3 ubiquitin ligase SCF complex subunit SKP1/ASK1 family protein	[more]
AT4G34210.1	2.2e-58	73.20	SKP1-like 11	[more]
AT4G34470.1	8.2e-58	73.38	SKP1-like 12	[more]
AT1G20140.1	2.6e-56	68.13	SKP1-like 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001232	S-phase kinase-associated protein 1-like	SMART	SM00512	skp1_3	coord: 4..105 e-value: 9.0E-53 score: 191.3
IPR011333	SKP1/BTB/POZ domain superfamily	GENE3D	3.30.710.10	Potassium Channel Kv1.1; Chain A	coord: 1..155 e-value: 2.7E-75 score: 254.0
IPR011333	SKP1/BTB/POZ domain superfamily	SUPERFAMILY	54695	POZ domain	coord: 5..65
IPR016897	S-phase kinase-associated protein 1	PIRSF	PIRSF028729	SCF_Skp	coord: 1..155 e-value: 7.5E-68 score: 225.4
IPR016897	S-phase kinase-associated protein 1	PANTHER	PTHR11165	SKP1	coord: 1..155
IPR016072	SKP1 component, dimerisation	PFAM	PF01466	Skp1	coord: 106..153 e-value: 1.9E-30 score: 104.6
IPR016073	SKP1 component, POZ domain	PFAM	PF03931	Skp1_POZ	coord: 5..64 e-value: 2.0E-30 score: 104.7
None	No IPR available	PANTHER	PTHR11165:SF145	PUTATIVE-RELATED	coord: 1..155
None	No IPR available	CDD	cd18322	BTB_POZ_SKP1	coord: 6..120 e-value: 4.96866E-60 score: 179.71
IPR036296	SKP1-like, dimerisation domain superfamily	SUPERFAMILY	81382	Skp1 dimerisation domain-like	coord: 78..153

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19462-RA	Carg19462-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
biological_process	GO:0031146	SCF-dependent proteasomal ubiquitin-dependent protein catabolic process
biological_process	GO:0006511	ubiquitin-dependent protein catabolic process
cellular_component	GO:0005737	cytoplasm
cellular_component	GO:0005634	nucleus
molecular_function	GO:0097602	cullin family protein binding