Carg19461 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg19461 vs. NCBI nr
Match: KAG7015830.1 (Protein goliath, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 305.8 bits (782), Expect = 1.9e-79
Identity = 142/142 (100.00%), Postives = 142/142 (100.00%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDCVAALSSPEKALEGET 60
           MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDCVAALSSPEKALEGET
Sbjct: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDCVAALSSPEKALEGET 60

Query: 61  CCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHED 120
           CCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHED
Sbjct: 61  CCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHED 120

Query: 121 GSNEMLTDEMVIWFSSFHTSGF 143
           GSNEMLTDEMVIWFSSFHTSGF
Sbjct: 121 GSNEMLTDEMVIWFSSFHTSGF 142

BLAST of Carg19461 vs. NCBI nr
Match: KAG6577791.1 (Protein goliath, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 303.5 bits (776), Expect = 9.4e-79
Identity = 141/142 (99.30%), Postives = 141/142 (99.30%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDCVAALSSPEKALEGET 60
           MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRP TPDDCVAALSSPEKALEGET
Sbjct: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPMTPDDCVAALSSPEKALEGET 60

Query: 61  CCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHED 120
           CCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHED
Sbjct: 61  CCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHED 120

Query: 121 GSNEMLTDEMVIWFSSFHTSGF 143
           GSNEMLTDEMVIWFSSFHTSGF
Sbjct: 121 GSNEMLTDEMVIWFSSFHTSGF 142

BLAST of Carg19461 vs. NCBI nr
Match: XP_038906276.1 (E3 ubiquitin-protein ligase RNF167-like [Benincasa hispida])

HSP 1 Score: 237.3 bits (604), Expect = 8.3e-59
Identity = 113/147 (76.87%), Postives = 127/147 (86.39%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDC-VAALSSPEKALEGE 60
           MVFNK+FFFFYNN +GFR+    KQATPLP   + SLRP TPDDC +  LSSPEKALEGE
Sbjct: 1   MVFNKVFFFFYNNFMGFRSRLRCKQATPLPHGGNLSLRPITPDDCAIVTLSSPEKALEGE 60

Query: 61  TCCVCLSCMGNK--DKDEGIG--TSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGG 120
           TCCVCLS MGNK  D+D+G+G  TSVLPC HEFHK+C+DRWFEECRRTCPICRYSMEGGG
Sbjct: 61  TCCVCLSSMGNKDGDRDKGVGTSTSVLPCLHEFHKVCVDRWFEECRRTCPICRYSMEGGG 120

Query: 121 NNHEDGSNEMLTDEMVIWFSSFHTSGF 143
            +HEDG+N++LTDEMVIWFSSFHTSGF
Sbjct: 121 -SHEDGTNQILTDEMVIWFSSFHTSGF 146

BLAST of Carg19461 vs. NCBI nr
Match: KAE8649999.1 (hypothetical protein Csa_010276, partial [Cucumis sativus])

HSP 1 Score: 230.3 bits (586), Expect = 1.0e-56
Identity = 109/140 (77.86%), Postives = 123/140 (87.86%), Query Frame = 0

Query: 6   LFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDC-VAALSSPEKALEGETCCVC 65
           +FFFFYNNLIGFR+ P  KQATPLPRS +SSLRP   DDC + ALSS EKALEGETCCVC
Sbjct: 1   VFFFFYNNLIGFRSRPRFKQATPLPRSGNSSLRPLPTDDCTILALSSLEKALEGETCCVC 60

Query: 66  LSCMGNKDK--DEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHEDGS 125
           LS MGN D+  D+G+GTSVLPC HEFHK+C++RWFEECRRTCPICRYSMEGGG +HED +
Sbjct: 61  LSSMGNTDRDGDKGVGTSVLPCLHEFHKVCVERWFEECRRTCPICRYSMEGGG-SHEDET 120

Query: 126 NEMLTDEMVIWFSSFHTSGF 143
           N++LTDEMVIWFSSFHTSGF
Sbjct: 121 NQILTDEMVIWFSSFHTSGF 139

BLAST of Carg19461 vs. NCBI nr
Match: KAA0052944.1 (E3 ubiquitin-protein ligase RNF13 [Cucumis melo var. makuwa] >TYK11400.1 E3 ubiquitin-protein ligase RNF13 [Cucumis melo var. makuwa])

HSP 1 Score: 226.9 bits (577), Expect = 1.1e-55
Identity = 112/149 (75.17%), Postives = 123/149 (82.55%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPT-LKQATPLPRSADSSLRPTTPDDC-VAALSSPEKALEG 60
           MVFNK+FFFFYNN IGFR+ PT  KQATPLP   +SSLRP   DDC + ALSS EKALEG
Sbjct: 1   MVFNKVFFFFYNNFIGFRSRPTRFKQATPLPHGGNSSLRPMPTDDCTIIALSSLEKALEG 60

Query: 61  ETCCVCLSCMGNK----DKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSME-G 120
           ETCCVCLS MGN     DK  G GTSVLPC HEFHK+C++RWFEECRRTCPICRYSME G
Sbjct: 61  ETCCVCLSSMGNTDGDGDKGVGNGTSVLPCLHEFHKVCVERWFEECRRTCPICRYSMEGG 120

Query: 121 GGNNHEDGSNEMLTDEMVIWFSSFHTSGF 143
           GG +HED +N++LTDEMVIWFSSFHTSGF
Sbjct: 121 GGGSHEDETNQILTDEMVIWFSSFHTSGF 149

BLAST of Carg19461 vs. ExPASy Swiss-Prot
Match: Q91XF4 (E3 ubiquitin-protein ligase RNF167 OS=Mus musculus OX=10090 GN=Rnf167 PE=2 SV=1)

HSP 1 Score: 57.4 bits (137), Expect = 1.5e-07
Identity = 23/67 (34.33%), Postives = 37/67 (55.22%), Query Frame = 0

Query: 53  EKALEGETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSME 112
           +K  E + C +CL      + ++G    VLPC+H +H  C+D W  + R+TCPIC+  + 
Sbjct: 222 QKGDEYDVCAICLD-----EYEDGDKLRVLPCAHAYHSRCVDPWLTQTRKTCPICKQPVH 281

Query: 113 GGGNNHE 120
            G  + E
Sbjct: 282 RGPGDEE 283

BLAST of Carg19461 vs. ExPASy Swiss-Prot
Match: Q5XIL0 (E3 ubiquitin-protein ligase RNF167 OS=Rattus norvegicus OX=10116 GN=Rnf167 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-07
Identity = 22/67 (32.84%), Postives = 37/67 (55.22%), Query Frame = 0

Query: 53  EKALEGETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSME 112
           +K  E + C +CL      + ++G    +LPC+H +H  C+D W  + R+TCPIC+  + 
Sbjct: 222 QKGDEYDVCAICLD-----EYEDGDKLRILPCAHAYHSRCVDPWLTQTRKTCPICKQPVH 281

Query: 113 GGGNNHE 120
            G  + E
Sbjct: 282 RGPGDEE 283

BLAST of Carg19461 vs. ExPASy Swiss-Prot
Match: Q4R6Y5 (E3 ubiquitin-protein ligase ZNRF4 OS=Macaca fascicularis OX=9541 GN=ZNRF4 PE=2 SV=1)

HSP 1 Score: 57.0 bits (136), Expect = 2.0e-07
Identity = 25/72 (34.72%), Postives = 42/72 (58.33%), Query Frame = 0

Query: 59  ETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEEC-RRTCPICRYSMEGGGNN 118
           + C +CL      + +EG    +LPCSH +H  C+D WF +  RR+CP+C+ S+ G  ++
Sbjct: 307 DLCAICLD-----EYEEGDQLKILPCSHTYHCKCIDPWFSQAPRRSCPVCKQSVAGTEDS 366

Query: 119 HEDGSNEMLTDE 130
             D + +  +DE
Sbjct: 367 F-DSTTDSFSDE 372

BLAST of Carg19461 vs. ExPASy Swiss-Prot
Match: Q9NVW2 (E3 ubiquitin-protein ligase RLIM OS=Homo sapiens OX=9606 GN=RLIM PE=1 SV=3)

HSP 1 Score: 56.6 bits (135), Expect = 2.6e-07
Identity = 26/58 (44.83%), Postives = 34/58 (58.62%), Query Frame = 0

Query: 59  ETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGN 117
           +TC VC++     +  EG     LPCSHE+H  C+DRW  E   TCPICR ++   GN
Sbjct: 568 KTCSVCIT-----EYTEGNKLRKLPCSHEYHVHCIDRWLSE-NSTCPICRRAVLASGN 619

BLAST of Carg19461 vs. ExPASy Swiss-Prot
Match: Q9WTV7 (E3 ubiquitin-protein ligase RLIM OS=Mus musculus OX=10090 GN=Rlim PE=1 SV=2)

HSP 1 Score: 56.6 bits (135), Expect = 2.6e-07
Identity = 26/58 (44.83%), Postives = 34/58 (58.62%), Query Frame = 0

Query: 59  ETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGN 117
           +TC VC++     +  EG     LPCSHE+H  C+DRW  E   TCPICR ++   GN
Sbjct: 544 KTCSVCIT-----EYTEGNKLRKLPCSHEYHVHCIDRWLSE-NSTCPICRRAVLSSGN 595

BLAST of Carg19461 vs. ExPASy TrEMBL
Match: A0A0A0L4W7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002620 PE=4 SV=1)

HSP 1 Score: 239.6 bits (610), Expect = 8.1e-60
Identity = 113/145 (77.93%), Postives = 128/145 (88.28%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPDDC-VAALSSPEKALEGE 60
           M+FNK+FFFFYNNLIGFR+ P  KQATPLPRS +SSLRP   DDC + ALSS EKALEGE
Sbjct: 1   MIFNKVFFFFYNNLIGFRSRPRFKQATPLPRSGNSSLRPLPTDDCTILALSSLEKALEGE 60

Query: 61  TCCVCLSCMGNKDK--DEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNN 120
           TCCVCLS MGN D+  D+G+GTSVLPC HEFHK+C++RWFEECRRTCPICRYSMEGGG +
Sbjct: 61  TCCVCLSSMGNTDRDGDKGVGTSVLPCLHEFHKVCVERWFEECRRTCPICRYSMEGGG-S 120

Query: 121 HEDGSNEMLTDEMVIWFSSFHTSGF 143
           HED +N++LTDEMVIWFSSFHTSGF
Sbjct: 121 HEDETNQILTDEMVIWFSSFHTSGF 144

BLAST of Carg19461 vs. ExPASy TrEMBL
Match: A0A5D3CJ45 (E3 ubiquitin-protein ligase RNF13 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00530 PE=4 SV=1)

HSP 1 Score: 226.9 bits (577), Expect = 5.4e-56
Identity = 112/149 (75.17%), Postives = 123/149 (82.55%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPT-LKQATPLPRSADSSLRPTTPDDC-VAALSSPEKALEG 60
           MVFNK+FFFFYNN IGFR+ PT  KQATPLP   +SSLRP   DDC + ALSS EKALEG
Sbjct: 1   MVFNKVFFFFYNNFIGFRSRPTRFKQATPLPHGGNSSLRPMPTDDCTIIALSSLEKALEG 60

Query: 61  ETCCVCLSCMGNK----DKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSME-G 120
           ETCCVCLS MGN     DK  G GTSVLPC HEFHK+C++RWFEECRRTCPICRYSME G
Sbjct: 61  ETCCVCLSSMGNTDGDGDKGVGNGTSVLPCLHEFHKVCVERWFEECRRTCPICRYSMEGG 120

Query: 121 GGNNHEDGSNEMLTDEMVIWFSSFHTSGF 143
           GG +HED +N++LTDEMVIWFSSFHTSGF
Sbjct: 121 GGGSHEDETNQILTDEMVIWFSSFHTSGF 149

BLAST of Carg19461 vs. ExPASy TrEMBL
Match: A0A6J1CW69 (E3 ubiquitin-protein ligase SDIR1-like OS=Momordica charantia OX=3673 GN=LOC111014786 PE=4 SV=1)

HSP 1 Score: 181.4 bits (459), Expect = 2.6e-42
Identity = 98/148 (66.22%), Postives = 110/148 (74.32%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIGFRTSPTLKQATPLPRSADSSLRPTTPD-----DCVAALSSPEKA 60
           MVFN++FFFFY N IGFR  P LKQ T LP   DS+LR    D     DCV  LSS  KA
Sbjct: 1   MVFNRVFFFFY-NFIGFRGRPKLKQKTLLPHGGDSTLRQMVEDESRIPDCV-TLSSSGKA 60

Query: 61  LEGE-TCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGG 120
           LEGE  CCVCLS +     +EGI TSVLPC HEFHK+C+DRWFEE RRTCPICRYSMEG 
Sbjct: 61  LEGEMCCCVCLSSI-----EEGIETSVLPCLHEFHKVCVDRWFEERRRTCPICRYSMEGA 120

Query: 121 GNNHEDGSNEMLTDEMVIWFSSFHTSGF 143
           G  +E+G+ ++LTDEMVIWFSSFHTSGF
Sbjct: 121 GQGNENGT-QILTDEMVIWFSSFHTSGF 140

BLAST of Carg19461 vs. ExPASy TrEMBL
Match: A0A7N2R4T7 (RING-type domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 119.8 bits (299), Expect = 9.3e-24
Identity = 71/147 (48.30%), Postives = 87/147 (59.18%), Query Frame = 0

Query: 1   MVFNKLFFFFYNNLIG----FRTSPTLKQATPLPRSADSSLRPTTPDDCVAALSSPEKAL 60
           MVF KL  F YN+LIG     R  PT  +A  L       L   + D+ V      E  +
Sbjct: 1   MVFKKLVCFIYNHLIGNGDHLRAQPTFWEAPSL-------LHKVSLDE-VEVEVEVEVEV 60

Query: 61  EGETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEE-CRRTCPICRYSMEGGG 120
           EGE CCVCLS +  K ++E      LPC HEFHKIC+DRWF   CR+TCP+CR+SM G  
Sbjct: 61  EGEVCCVCLSRL-KKGQEE---MRTLPCLHEFHKICVDRWFNHVCRKTCPVCRFSMGGED 120

Query: 121 NNHEDGSNEMLTDEMVIWFSSFHTSGF 143
             H   + ++LTDEMVIWFSSFH +GF
Sbjct: 121 KFH---TQQVLTDEMVIWFSSFHVAGF 132

BLAST of Carg19461 vs. ExPASy TrEMBL
Match: A0A6A1UIA7 (Protein preY, mitochondrial OS=Morella rubra OX=262757 GN=CJ030_MR0G008870 PE=4 SV=1)

HSP 1 Score: 114.4 bits (285), Expect = 3.9e-22
Identity = 65/135 (48.15%), Postives = 79/135 (58.52%), Query Frame = 0

Query: 14  LIGFRTSPTLKQATPLPRSADS--SLRPTTPDDCVAA---LSSPEKALEGE-TCCVCLSC 73
           LIG R  P LK+A     S  +   L     D  ++A    SS  +  EGE  CCVCLS 
Sbjct: 101 LIGLRAQPALKEAPSSSYSVINWDDLDEVKEDPTMSARGSSSSSGEVHEGEQQCCVCLSR 160

Query: 74  MGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHEDGSNEMLT 133
           +      +G  T +LPC HEFHK+C+DRWF  CR+TCP+CR+SM G  N       E LT
Sbjct: 161 L-----KKGQDTRILPCLHEFHKVCVDRWFNSCRKTCPVCRFSMGGEDNCR---VQEALT 220

Query: 134 DEMVIWFSSFHTSGF 143
           DEMVIWFSSFH +GF
Sbjct: 221 DEMVIWFSSFHVAGF 227

BLAST of Carg19461 vs. TAIR 10
Match: AT3G51325.1 (RING/U-box superfamily protein )

HSP 1 Score: 73.2 bits (178), Expect = 1.9e-13
Identity = 37/83 (44.58%), Postives = 48/83 (57.83%), Query Frame = 0

Query: 57  EGETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRR-TCPICRYSMEGGG 116
           E E C VCL  M  KD  +      LPCSHEFH +C+D WF   R+  CP+CR+S     
Sbjct: 22  EEECCSVCLMRMEAKDVIKS-----LPCSHEFHSLCVDTWFNVSRKICCPLCRFS----- 81

Query: 117 NNHEDGSNEMLTDEMVIWFSSFH 139
                 +  +LTDE+++WFSSFH
Sbjct: 82  -----PTTILLTDELLLWFSSFH 89

BLAST of Carg19461 vs. TAIR 10
Match: AT4G32600.1 (RING/U-box superfamily protein )

HSP 1 Score: 56.6 bits (135), Expect = 1.9e-08
Identity = 39/110 (35.45%), Postives = 56/110 (50.91%), Query Frame = 0

Query: 21  PTLKQATPLPRS-ADSSLRPTTPDDCVAALSSPEKALEGE--TCCVCLSCMGNKDKDEGI 80
           PT K      RS  D +   T+    VAA +  E+A+ GE   CC+CL+   N ++    
Sbjct: 320 PTHKFKLKKSRSNGDDNGSSTSEGGVVAAGTDNERAISGEDAVCCICLAKYANNEE---- 379

Query: 81  GTSVLPCSHEFHKICLDRWFEECRRTCPICRYSMEGGGNNHEDGSNEMLT 128
               LPCSH FHK C+D+W  +   +CP+C+   E G  N +  S  +LT
Sbjct: 380 -LRELPCSHFFHKECVDKWL-KINASCPLCK--SEVGEKNSDLTSQGILT 421

BLAST of Carg19461 vs. TAIR 10
Match: AT1G35630.1 (Protease-associated (PA) RING/U-box zinc finger family protein )

HSP 1 Score: 56.2 bits (134), Expect = 2.4e-08
Identity = 21/66 (31.82%), Postives = 35/66 (53.03%), Query Frame = 0

Query: 53  EKALEGETCCVCLS--CMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICRYS 112
           E++    TC +C+   C+G K         +LPC H++H +C+D W   CR  CP+C+ +
Sbjct: 225 EESSTSVTCAICIDDYCVGEK-------LRILPCKHKYHAVCIDSWLGRCRSFCPVCKQN 283

Query: 113 MEGGGN 117
              G +
Sbjct: 285 PRTGND 283

BLAST of Carg19461 vs. TAIR 10
Match: AT5G45290.1 (RING/U-box superfamily protein )

HSP 1 Score: 55.8 bits (133), Expect = 3.2e-08
Identity = 21/45 (46.67%), Postives = 29/45 (64.44%), Query Frame = 0

Query: 64  CLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICR 109
           C  C+   ++ + I T  LPC HEFHK C+D+W +E  R CP+CR
Sbjct: 490 CYICLVEYEEADSIRT--LPCHHEFHKTCVDKWLKEIHRVCPLCR 532

BLAST of Carg19461 vs. TAIR 10
Match: AT1G24580.1 (RING/U-box superfamily protein )

HSP 1 Score: 54.7 bits (130), Expect = 7.1e-08
Identity = 24/56 (42.86%), Postives = 34/56 (60.71%), Query Frame = 0

Query: 53  EKALEGETCCVCLSCMGNKDKDEGIGTSVLPCSHEFHKICLDRWFEECRRTCPICR 109
           E+  +G  CCVCL   G K+++E   + ++ C H FH+ CLD WF     TCP+CR
Sbjct: 59  EEEEKGVECCVCL--CGFKEEEE--VSELVSCKHFFHRACLDNWFGNNHTTCPLCR 110