Carg19452 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19452
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	bifunctional nuclease 2-like
Location	Carg_Chr16: 10057636 .. 10060994 (+)
RNA-Seq Expression	Carg19452
Synteny	Carg19452

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CTCTCTCTCTCAATCGATTCAGCCGATTGAACACAAACTCTCTCTGTAGCAGCGAAAAAGAAGCGGTTTCAGGCCAACCCCACTTCAAAACTCTCCCCCTTTCTCCTTCAATCTCCTTCTTCTTCATCTTCCATCGCCATTTTTCTCTGTAATTCCTCCATTACAATGCTGGGTTTAGCCCCTCACTTCTCTCTTCGTACCCTTTTCCCCGGATCCGCCATAGCCATGGATCATCCCTCTGCTTCTCGCCCTTCCTCTTCCCTTCACTTCTCTGTTCTCCGATCGCGGCCCAATGCGGTTAGCTCTCGCCGACCCAAGTCGATCCTCGTTTCTTGCCACTCTTCTCGTGATAGATCCACCCACAGTGACGATGATTCCCATCAGGATTACTTCGAAGCTTCGTTTCTTGTTTCAGGTTCTTTGATTTTACTTTCTGGGTTTCTTTTCTGAAAATCGCGATGAAATCGATTGTGGGTGTTTATGATTCCTGTATTTGTTTCATAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGACGCGAATTTCAATCAGATTGAATCGCGAGTCAATTCCCCTTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTCCTCAAAATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGTATATTGGATCAAAACTTTGTCGTGATTGTGTTTGTGTTTCTTATGAATTTGGCTGTAAGTTTTGGACTCTGTTTGCAGGGGAATATGCTATTGAGAAACTTATAGATTCTCACCTTGGGATTGAAAATGGGGTTCGTTTTCTTTGGCTTTGTTCTTCATATTTTTGGCTTTTTTTTTTTTTTTGTTCTTTGTTCTTCATATTTTTGGTTTTATTTGGCTTTGTTCTTCATATTTCTGGTTTTTCATACCTTGTGGTTGGTATTAATTGTCTAATGATCATGTTACAGGAAGCCCCAGATCTCTTTCAGTTTATACAAGACCTTATGGTGAAAGTTGGCTATGAAGTATGGAGCTACCTTCTAACTTAACAACTAAATACATGTTCAATCTTTATTATTATTTCAGTTCCACGATGTGGTTTGGACATTCGGATAGTAGTCCGTGCCTTATCGACTAAGTTATGCTTAGATTGGTCGTAGATTTTATATTTGGTTTAGTGTTCTCTTTGAGAGCTTTCGTGACGTGGGTGTGAAGGACACTAGGATGGAGTTTTTTCGGCTTTTGATTATGCGCTTCGGAATTTTTACTTCTATACAAGAACCAATAGGTTGTGGTGTAAATCTGCTAGTTTTGTTTGTTTTAACTTGTGTCGTGACGGATGTATACCGATGGGGGACGAGGTTATTCTCAAGAATGTCAGTCTCTTTGCCCCTTGCATTTCCAAGTATTATATGATTTGAGGCTTAGTTCTTCTTCTATTGATGGTTAATAAATTGTGGGTGGGTAGTGTTTAGTGTTAGGAACCTTCCTTCCTTCATTGACTTGTTTCTTTAATGTAATTGAGTGGTAAAATGAGAATCTAGAAGCAATGTAAAGGTTCTGAATAAACTATGAAACAGGAGTTTCGAGCCGTAATCTAACGTCGTTTTATGCTGTTGTGCGTATAGGTCACGACAGCGAGAATTACTGAAAGAGTGGTGAACACTTACTATGCGCGGCTCTTCTTGAGAAAGGTTAGCTCTTGTAAATTCAGTCACAATTAGCTAATACTGTTCTTGAAGTTTTCAATTTATGTCGGGTTTTTGTTTTCTTTGTCACACTACTATTATCATCTGCAGGATGGGGAAAACGAGACGTTAAGTGTGGACGCACGCCCCTCAGATGCCTTAAATATTGCATATCGGTGTAAGGTAGATATTCCTACCATCGACTGCAGAGATCTTTACAGAACCTCTGCGAGCTGCTGGGATGACTTAGTTGGTTTTAAAATGAAGTACATGTGAGATCCCACGTCGGTTGGGGAGGAGAACGAAGCATTCTTTATAAAGGTGCGGAAACCTCTCCCTAGCAGACACGTTTTAAAAACCTTGAAGGGAAGCTCAAAGAGGACAATATCTGCTAGCGGTGGGCTTGAGTTGTTACAAATGGTATCAGAGCTAGACACTGGGTGATGTGCCAATGAGGAGGTTGAGCCCCAAATGTCAGCAAGGATGTTGGGCCCCAAAGGGGGTGGATTGGGGGGTCCCACGTCAATTGGAGAAGGGAACGAGTGCCAACGAGGACATTGGGTTCTGAAGGGGGGTAGATTGTGAGATCCCACATCGGTTGGGGAGGAGAACGAAGCATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCAGACACGTTTTAAAACCTTGAGAGGAAACTCGAAAGGGAAAGCCTAAAGAAGAAAATATCGGCTAATTTAATGCTTTACTTTGCACCCTCTCATTCTTACTTCACTTAAACTTTTCTGCGCTTACGCCATTTTCCTGTTTAACGAATATGCAGATACCGGTACTCGTTAGTAAGCAGATTGTCTATGAGGATGCGATAAGAGTTAGTTTCGGGTTTGGAAGAGTGCGCAAGAGGAAGTCATGTTTTGATGTTCTACTTGACTGGTATAAGTGCAAGCATCGTTGGCTTATTCGATATACATCGTTTCTCAATATCTAAAAACGATAGTTTCTTGTTCAGTGCTGCAGATGGTCCAGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAACATGAAAATGGCTATTTACGAAGAACGATACAAAGATGCAGGTATAAGTAGAAAAGATTTCAACCATATCCTATCCAGACGTTCTGTTTCGATCATGTTCATAAAATGAAGGTATTTATGCTTGACGTTTGTTTTCTTCAGCTATGTGGAGAGATAAGTTAACAAGGCTTCGAAAATCTGTACATGAGGCGTAACGTCGAACTGCACTTGGACGTGTTGTTCCAGTAAGTAACTTCCTGTAATCAACCGATGTACGGGGAAAGGTACCACCCGAAGAAACATGTTTGAATTTTTCTGATACTGATCCCTAAACAGGGCGACTGTTCTGTTGGGTAGGAATTGTTCGCTTGCAGGCATCTCTTAGAACGTCGAGTTCAGCATTGGATGGATTGGGGACACAGAGCGGACTGCTACGCATCGTTGCAGAAAGTTATCGATAAAGATGGTCGGTCTGTACAGTTACAGTTAAAAGAGGTATGTAAATACTACATTTGGGTAGTGAATGTGTTGTATCTCTAAGATGTGAATGTTATGGTTGATATTCATTCTTCACATAGAGAGAGAGAGAGAGAGAGATGCTGAATGTGAAAGGAAGCTTTTGTATATCTTAACAAAGATCCTTTTG

mRNA sequence

CTCTCTCTCTCAATCGATTCAGCCGATTGAACACAAACTCTCTCTGTAGCAGCGAAAAAGAAGCGGTTTCAGGCCAACCCCACTTCAAAACTCTCCCCCTTTCTCCTTCAATCTCCTTCTTCTTCATCTTCCATCGCCATTTTTCTCTGTAATTCCTCCATTACAATGCTGGGTTTAGCCCCTCACTTCTCTCTTCGTACCCTTTTCCCCGGATCCGCCATAGCCATGGATCATCCCTCTGCTTCTCGCCCTTCCTCTTCCCTTCACTTCTCTGTTCTCCGATCGCGGCCCAATGCGGTTAGCTCTCGCCGACCCAAGTCGATCCTCGTTTCTTGCCACTCTTCTCGTGATAGATCCACCCACAGTGACGATGATTCCCATCAGGATTACTTCGAAGCTTCGTTTCTTGTTTCAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGACGCGAATTTCAATCAGATTGAATCGCGAGTCAATTCCCCTTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTCCTCAAAATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTCTCACCTTGGGATTGAAAATGGGGAAGCCCCAGATCTCTTTCAGTTTATACAAGACCTTATGGTGAAAGTTGGCTATGAAGTCACGACAGCGAGAATTACTGAAAGAGTGGTGAACACTTACTATGCGCGGCTCTTCTTGAGAAAGGATGGGGAAAACGAGACGTTAAGTGTGGACGCACGCCCCTCAGATGCCTTAAATATTGCATATCGGTGTAAGATACCGGTACTCGTTAGTAAGCAGATTGTCTATGAGGATGCGATAAGAGTTAGTTTCGGGTTTGGAAGAGTGCGCAAGAGGAAGTCATGTTTTGATGTTCTACTTGACTGTGCTGCAGATGGTCCAGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAACATGAAAATGGCTATTTACGAAGAACGATACAAAGATGCAGCTATGTGGAGAGATAAGTTAACAAGGCTTCGAAAATCTGTACATGAGGCGTAACGTCGAACTGCACTTGGACGTGTTGTTCCAGGCGACTGTTCTGTTGGGTAGGAATTGTTCGCTTGCAGGCATCTCTTAGAACGTCGAGTTCAGCATTGGATGGATTGGGGACACAGAGCGGACTGCTACGCATCGTTGCAGAAAGTTATCGATAAAGATGGTCGGTCTGTACAGTTACAGTTAAAAGAGGTATGTAAATACTACATTTGGGTAGTGAATGTGTTGTATCTCTAAGATGTGAATGTTATGGTTGATATTCATTCTTCACATAGAGAGAGAGAGAGAGAGAGATGCTGAATGTGAAAGGAAGCTTTTGTATATCTTAACAAAGATCCTTTTG

Coding sequence (CDS)

ATGCTGGGTTTAGCCCCTCACTTCTCTCTTCGTACCCTTTTCCCCGGATCCGCCATAGCCATGGATCATCCCTCTGCTTCTCGCCCTTCCTCTTCCCTTCACTTCTCTGTTCTCCGATCGCGGCCCAATGCGGTTAGCTCTCGCCGACCCAAGTCGATCCTCGTTTCTTGCCACTCTTCTCGTGATAGATCCACCCACAGTGACGATGATTCCCATCAGGATTACTTCGAAGCTTCGTTTCTTGTTTCAGAGACTATCTATCACTATCGAATGTGGAAGAAGAGGTTTCAAGAGGACGCGAATTTCAATCAGATTGAATCGCGAGTCAATTCCCCTTCACTTGGACTGGGGTTTTTTAGACGTTTTCGAAGCCCTACTGTGTTCCTCAAAATTTCTTGCAATGGTGACTTTCTACTGCCAATTGTCGTAGGGGAATATGCTATTGAGAAACTTATAGATTCTCACCTTGGGATTGAAAATGGGGAAGCCCCAGATCTCTTTCAGTTTATACAAGACCTTATGGTGAAAGTTGGCTATGAAGTCACGACAGCGAGAATTACTGAAAGAGTGGTGAACACTTACTATGCGCGGCTCTTCTTGAGAAAGGATGGGGAAAACGAGACGTTAAGTGTGGACGCACGCCCCTCAGATGCCTTAAATATTGCATATCGGTGTAAGATACCGGTACTCGTTAGTAAGCAGATTGTCTATGAGGATGCGATAAGAGTTAGTTTCGGGTTTGGAAGAGTGCGCAAGAGGAAGTCATGTTTTGATGTTCTACTTGACTGTGCTGCAGATGGTCCAGATTTCTTGTCCGAGGAACTCGATATGCTGAAGAACATGAAAATGGCTATTTACGAAGAACGATACAAAGATGCAGCTATGTGGAGAGATAAGTTAACAAGGCTTCGAAAATCTGTACATGAGGCGTAA

Protein sequence

MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTRLRKSVHEA

Homology

[Close]

BLAST of Carg19452 vs. NCBI nr
Match: KAG7015839.1 (Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 619.4 bits (1596), Expect = 1.7e-173
Identity = 310/310 (100.00%), Postives = 310/310 (100.00%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120
           RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180
           RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
           VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300
           IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TRLRKSVHEA 311
           TRLRKSVHEA
Sbjct: 301 TRLRKSVHEA 310

[Close]

BLAST of Carg19452 vs. NCBI nr
Match: XP_022923551.1 (bifunctional nuclease 2-like [Cucurbita moschata] >XP_022923553.1 bifunctional nuclease 2-like [Cucurbita moschata])

HSP 1 Score: 615.5 bits (1586), Expect = 2.4e-172
Identity = 308/310 (99.35%), Postives = 309/310 (99.68%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120
           RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSP LGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180
           RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
           VTTARITERVVNTY+ARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300
           IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TRLRKSVHEA 311
           TRLRKSVHEA
Sbjct: 301 TRLRKSVHEA 310

[Close]

BLAST of Carg19452 vs. NCBI nr
Match: XP_022965292.1 (bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965294.1 bifunctional nuclease 2-like [Cucurbita maxima])

HSP 1 Score: 610.1 bits (1572), Expect = 1.0e-170
Identity = 304/310 (98.06%), Postives = 307/310 (99.03%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGL PHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAV SRRPKSILVSCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120
           RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQED NFNQIESRVNSPSLGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180
           RF+SPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE
Sbjct: 121 RFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
           VTTARITERVVNTY+ARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300
           IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TRLRKSVHEA 311
           T+LRKSVHEA
Sbjct: 301 TKLRKSVHEA 310

[Close]

BLAST of Carg19452 vs. NCBI nr
Match: XP_023553147.1 (bifunctional nuclease 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_023553148.1 bifunctional nuclease 2-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 607.1 bits (1564), Expect = 8.7e-170
Identity = 302/310 (97.42%), Postives = 307/310 (99.03%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAV SRRPKSIL+SCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120
           RDRST SDDDSHQDYFEASFLVSETIYHYRMWKKRFQED NFNQIESRVNSPSLGLGFFR
Sbjct: 61  RDRSTPSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180
           RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID+HLGIENGEAPDLFQFIQDLMVKVGYE
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDAHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
           VTTARITERVVNTY+AR+FLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARIFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300
           IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TRLRKSVHEA 311
           T+LRKSVHEA
Sbjct: 301 TKLRKSVHEA 310

[Close]

BLAST of Carg19452 vs. NCBI nr
Match: KAG6577800.1 (Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 580.9 bits (1496), Expect = 6.7e-162
Identity = 290/290 (100.00%), Postives = 290/290 (100.00%), Query Frame = 0


Query: 21  MDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASF 80
           MDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASF
Sbjct: 1   MDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSSRDRSTHSDDDSHQDYFEASF 60

Query: 81  LVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSPTVFLKISCNGDFLLP 140
           LVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSPTVFLKISCNGDFLLP
Sbjct: 61  LVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSPTVFLKISCNGDFLLP 120

Query: 141 IVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYYARLFL 200
           IVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYYARLFL
Sbjct: 121 IVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYYARLFL 180

Query: 201 RKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVL 260
           RKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVL
Sbjct: 181 RKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVL 240

Query: 261 LDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTRLRKSVHEA 311
           LDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTRLRKSVHEA
Sbjct: 241 LDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTRLRKSVHEA 290

[Close]

BLAST of Carg19452 vs. ExPASy Swiss-Prot
Match: Q93VH2 (Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 129.4 bits (324), Expect = 7.0e-29
Identity = 100/304 (32.89%), Postives = 155/304 (50.99%), Query Frame = 0


Query: 9   SLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSI--LVSCHSSRDRSTH 68
           SLR  F G+    D   A+  +  LH             RR KSI  L S HS    ST 
Sbjct: 34  SLRKQFWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTA 93

Query: 69  SD-DDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSP 128
            + +++ +DY ++S L +  +           +D    Q+    N+P  G          
Sbjct: 94  ENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDG--RQLRCVHNNPQ-GGNLPNYAPHS 153

Query: 129 TVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTAR 188
            + LK+      LLPI+V E     L+ +   ++    P ++Q ++D++ K+GYEV   R
Sbjct: 154 AIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKDMVDKMGYEVRLVR 213

Query: 189 ITERVVNTYYARLFLRKDGE-NETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVS 248
           +T RV   Y+A L+L K G+ ++ +S D RPSDA+NIA RCK+P+ V+K + Y D +RV 
Sbjct: 214 VTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV- 273

Query: 249 FGFGRVRKRKSCFDVLLDCAADGPD----FLSEELDMLKNMKMAIYEERYKDAAMWRDKL 305
              G++ K+    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL
Sbjct: 274 IDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKL 320

[Close]

BLAST of Carg19452 vs. ExPASy Swiss-Prot
Match: Q6YZM6 (Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV=1)

HSP 1 Score: 126.7 bits (317), Expect = 4.5e-28
Identity = 76/201 (37.81%), Postives = 112/201 (55.72%), Query Frame = 0


Query: 109 VNSPSLGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQ 168
           V++ S G         P + L+I    + LLPI+V E     L+ +   +     P ++Q
Sbjct: 120 VHNNSQGRNIPESAPQPAIVLRIEDGSETLLPIIVLEMPSVLLMAAIRNVHIAR-PTIYQ 179

Query: 169 FIQDLMVKVGYEVTTARITERVVNTYYARLFLRKDGEN-ETLSVDARPSDALNIAYRCKI 228
            +++++ K+GYEV   RI +R+   Y A LFL K G++ E+++ D RPSDA+NIA RCK+
Sbjct: 180 VVKEMIDKMGYEVKLVRINKRIQEAYCAELFLTKVGDHTESITFDLRPSDAINIAVRCKV 239

Query: 229 PVLVSKQIVYEDAIRVSFGFGRVRKRKSCFDVLLDCAADGPD----FLSEELDMLKNMKM 288
           P+ V + + Y D IR S    R+       D LL    D PD      ++E  +++NM +
Sbjct: 240 PIQVHRSLAYSDGIR-SVEPARMAIAAGMSDGLLFTELDRPDGQPCVEAQEFGLIRNMLI 299

Query: 289 AIYEERYKDAAMWRDKLTRLR 305
           A  EERYKDAA WRDKL  LR
Sbjct: 300 AAVEERYKDAATWRDKLMLLR 318

[Close]

BLAST of Carg19452 vs. ExPASy Swiss-Prot
Match: Q9FWS6 (Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 124.8 bits (312), Expect = 1.7e-27
Identity = 72/185 (38.92%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 125 PTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTA 184
           P + LK+      LLPI+V E     L+ +   ++    P ++Q +++++ K+GYEV   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKEMVDKMGYEVRLV 193

Query: 185 RITERVVNTYYARLFLRKDGE-NETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRV 244
           R+T+RV   Y+A+LFL K G  +E +S D RPSDA+NIA RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 245 SFGFGRVRKRKSCFDVLLDCAADGPD----FLSEELDMLKNMKMAIYEERYKDAAMWRDK 304
               G++       D LL    D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 -IESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

[Close]

BLAST of Carg19452 vs. ExPASy Swiss-Prot
Match: Q5N8J3 (Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 70/182 (38.46%), Postives = 110/182 (60.44%), Query Frame = 0


Query: 127 VFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARI 186
           + LK+    D LLPI+V E     L+ +   I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 187 TERVVNTYYARLFLRKDG-ENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRV-- 246
           TE V + YY+RL+L K G E ET+S+D +PSDA+NIA+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 247 -SFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTR 305
            +     V   +  +   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of Carg19452 vs. ExPASy Swiss-Prot
Match: Q09LL3 (Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1)

HSP 1 Score: 122.1 bits (305), Expect = 1.1e-26
Identity = 70/182 (38.46%), Postives = 110/182 (60.44%), Query Frame = 0


Query: 127 VFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARI 186
           + LK+    D LLPI+V E     L+ +   I     P ++  ++++  ++GY V   RI
Sbjct: 145 IVLKMEDGSDLLLPIIVMETPSIMLLAALRNIRI-PRPTIYNVVKEMTERMGYAVRLVRI 204

Query: 187 TERVVNTYYARLFLRKDG-ENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRV-- 246
           TE V + YY+RL+L K G E ET+S+D +PSDA+NIA+RCK+P+ V+++I Y + ++V  
Sbjct: 205 TEMVHDAYYSRLYLAKIGNEEETISLDLKPSDAINIAFRCKVPIQVNRRIAYNNGLKVVQ 264

Query: 247 -SFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTR 305
            +     V   +  +   LD   D P F ++E D+++NM +A  EERYKDAA +RD+L  
Sbjct: 265 PTPSESYVSSDQFQY-TRLDRPDDQPCFEAQEFDLVRNMLVAAVEERYKDAAQYRDQLFM 324

[Close]

BLAST of Carg19452 vs. ExPASy TrEMBL
Match: A0A6J1E735 (bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 SV=1)

HSP 1 Score: 615.5 bits (1586), Expect = 1.2e-172
Identity = 308/310 (99.35%), Postives = 309/310 (99.68%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS
Sbjct: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120
           RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSP LGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPLLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180
           RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE
Sbjct: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
           VTTARITERVVNTY+ARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300
           IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TRLRKSVHEA 311
           TRLRKSVHEA
Sbjct: 301 TRLRKSVHEA 310

[Close]

BLAST of Carg19452 vs. ExPASy TrEMBL
Match: A0A6J1HJX9 (bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV=1)

HSP 1 Score: 610.1 bits (1572), Expect = 5.0e-171
Identity = 304/310 (98.06%), Postives = 307/310 (99.03%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGL PHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAV SRRPKSILVSCHSS
Sbjct: 1   MLGLVPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVRSRRPKSILVSCHSS 60

Query: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFR 120
           RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQED NFNQIESRVNSPSLGLGFFR
Sbjct: 61  RDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDTNFNQIESRVNSPSLGLGFFR 120

Query: 121 RFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180
           RF+SPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE
Sbjct: 121 RFQSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYE 180

Query: 181 VTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240
           VTTARITERVVNTY+ARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA
Sbjct: 181 VTTARITERVVNTYFARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDA 240

Query: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300
           IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL
Sbjct: 241 IRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKL 300

Query: 301 TRLRKSVHEA 311
           T+LRKSVHEA
Sbjct: 301 TKLRKSVHEA 310

[Close]

BLAST of Carg19452 vs. ExPASy TrEMBL
Match: A0A0A0L3L8 (BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV=1)

HSP 1 Score: 561.6 bits (1446), Expect = 2.0e-156
Identity = 282/312 (90.38%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGLAPHF L T+F GSAIAMDHPSASRPS SLHFS+ RSRPNAV SRRPKSIL+SCHSS
Sbjct: 1   MLGLAPHFCLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDD--SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGF 120
           RDRSTHSDDD  SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQ ESR NS SLGLGF
Sbjct: 61  RDRSTHSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQRESRANSNSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENGEAPD+FQFIQDL+VKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQDLIVKVG 180

Query: 181 YEVTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240
           YE  TARITERVVNTY+ARLFLRK+GE+E LSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRD 300
           DAIRVS+GFGRV +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTRLRKSVHEA 311
           KLT+LRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 312

[Close]

BLAST of Carg19452 vs. ExPASy TrEMBL
Match: A0A1S3BJU6 (bifunctional nuclease 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490665 PE=3 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 7.7e-156
Identity = 281/312 (90.06%), Postives = 295/312 (94.55%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSS 60
           MLGLAPHF L T+F GSAIAMDHPSASRPS SLHFS+ RSRPNAV SRRPKSIL+SCHSS
Sbjct: 1   MLGLAPHFRLPTIFFGSAIAMDHPSASRPSCSLHFSLHRSRPNAVRSRRPKSILISCHSS 60

Query: 61  RDRSTHSDDD--SHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGF 120
           RDRST+SDDD  SHQDYFEASFLVSETIYHYRMWKKRFQED NFNQ ESRVNSPSLGLGF
Sbjct: 61  RDRSTNSDDDDNSHQDYFEASFLVSETIYHYRMWKKRFQEDVNFNQRESRVNSPSLGLGF 120

Query: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVG 180
           FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENGEAPD+FQFIQ L+VKVG
Sbjct: 121 FRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGEAPDIFQFIQGLIVKVG 180

Query: 181 YEVTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYE 240
           YE  TARITERVVNTY+ARLFLRK+GE+E LSVDARPSDALNIAYRCKIPVLVSKQIV+E
Sbjct: 181 YEAITARITERVVNTYFARLFLRKEGESEMLSVDARPSDALNIAYRCKIPVLVSKQIVFE 240

Query: 241 DAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRD 300
           DAIRVS+GFGRV +RKSCFDVLLDCAADGPDFLSEELDMLKNMK+AIYEERYKDAAMWRD
Sbjct: 241 DAIRVSYGFGRVHERKSCFDVLLDCAADGPDFLSEELDMLKNMKIAIYEERYKDAAMWRD 300

Query: 301 KLTRLRKSVHEA 311
           KLT+LRKSVHEA
Sbjct: 301 KLTKLRKSVHEA 312

[Close]

BLAST of Carg19452 vs. ExPASy TrEMBL
Match: A0A6J1CVQ2 (bifunctional nuclease 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111014826 PE=3 SV=1)

HSP 1 Score: 519.6 bits (1337), Expect = 8.8e-144
Identity = 264/317 (83.28%), Postives = 283/317 (89.27%), Query Frame = 0


Query: 1   MLGLAPHFSLRTLFPGSAIAMDHPSASRP-------SSSLHFSVLRSRPNAVSSRRPKSI 60
           MLGL+PHF +RTLF G A+A DHP+ SRP       SSSLHFS L SR NA  SRR  SI
Sbjct: 1   MLGLSPHFCVRTLFAGFAMAGDHPTHSRPSAASWFASSSLHFSALPSRSNAGRSRRRNSI 60

Query: 61  LVSCHSSRDRSTHSDDDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPS 120
           LVSCHSSR RS  +DDDSHQDYFEASFLVSETI+HYRMWK+RFQEDANFNQ ESR NS S
Sbjct: 61  LVSCHSSRARSGSTDDDSHQDYFEASFLVSETIHHYRMWKRRFQEDANFNQRESRGNSHS 120

Query: 121 LGLGFFRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDL 180
           LGLGF RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLID  LGIENG++PDLF+FIQ+L
Sbjct: 121 LGLGFLRRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDCQLGIENGDSPDLFEFIQNL 180

Query: 181 MVKVGYEVTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSK 240
           MVK GYE+TTA+ITERVVNTY+ARLFLRKDGENE LSVDARPSDALNIAYRCKIPVLVSK
Sbjct: 181 MVKFGYEITTAKITERVVNTYFARLFLRKDGENEMLSVDARPSDALNIAYRCKIPVLVSK 240

Query: 241 QIVYEDAIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDA 300
           QIVYEDAI+VS+GFGRV +RKSCFDVLLD AADGPDFLS+ELDMLKNMK AIYEERYKDA
Sbjct: 241 QIVYEDAIKVSYGFGRVHERKSCFDVLLDSAADGPDFLSQELDMLKNMKRAIYEERYKDA 300

Query: 301 AMWRDKLTRLRKSVHEA 311
           AMWRDKLTRLRK +HEA
Sbjct: 301 AMWRDKLTRLRKLIHEA 317

[Close]

BLAST of Carg19452 vs. TAIR 10
Match: AT5G66050.1 (Wound-responsive family protein )

HSP 1 Score: 243.0 bits (619), Expect = 3.1e-64
Identity = 134/307 (43.65%), Postives = 192/307 (62.54%), Query Frame = 0


Query: 11  RTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSILVSCHSSRDRSTHSDDD 70
           R++    + +   PS+ R  + L+   LRS       RRPK   + C  S   S     D
Sbjct: 26  RSISASLSSSSSSPSSFRCCTPLNLKSLRSH------RRPKISHICCDGS-SISGSGLGD 85

Query: 71  SHQDYFEASFLVSETIYHYRMWKKRFQEDA-----------NFNQIESRVNSPSLGLGFF 130
              ++ +AS LV+ET  HY+M +  F++D+           +    ESRV    +GLGF 
Sbjct: 86  PDLEFLQASVLVAETSMHYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFL 145

Query: 131 RRFRSPTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGY 190
           R+F+ PT+FLKISC+GD+LLP++VG+ A+EKL+D  L     E PD FQF+  ++ K+GY
Sbjct: 146 RQFKQPTIFLKISCDGDYLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGY 205

Query: 191 EVTTARITERVVNTYYARLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYED 250
           EV   ++T R+VNTYYA L L K G+ E + +D+RPSDA+N+A  C+ P+ V+K IV E+
Sbjct: 206 EVKMVKLTGRIVNTYYASLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEE 265

Query: 251 AIRVSFGFGRVRKRKSCFDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDK 307
           AI++ +G GR +  K  F+V+LD A DGPD LSEEL +++NM +A  EERY DAAMWRD+
Sbjct: 266 AIKIGYG-GRPQSAKPVFNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDR 324

[Close]

BLAST of Carg19452 vs. TAIR 10
Match: AT5G66050.2 (Wound-responsive family protein )

HSP 1 Score: 227.3 bits (578), Expect = 1.7e-59
Identity = 112/230 (48.70%), Postives = 158/230 (68.70%), Query Frame = 0


Query: 88  HYRMWKKRFQEDA-----------NFNQIESRVNSPSLGLGFFRRFRSPTVFLKISCNGD 147
           HY+M +  F++D+           +    ESRV    +GLGF R+F+ PT+FLKISC+GD
Sbjct: 2   HYKMRRHGFRQDSMWQTSRPLPPFSIRASESRVGVLPIGLGFLRQFKQPTIFLKISCDGD 61

Query: 148 FLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTARITERVVNTYYA 207
           +LLP++VG+ A+EKL+D  L     E PD FQF+  ++ K+GYEV   ++T R+VNTYYA
Sbjct: 62  YLLPVIVGDAAVEKLLDVPLQGHTEECPDQFQFVSAVVDKLGYEVKMVKLTGRIVNTYYA 121

Query: 208 RLFLRKDGENETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVSFGFGRVRKRKSC 267
            L L K G+ E + +D+RPSDA+N+A  C+ P+ V+K IV E+AI++ +G GR +  K  
Sbjct: 122 SLCLGKPGDIEAICIDSRPSDAINVARACQAPIYVNKAIVLEEAIKIGYG-GRPQSAKPV 181

Query: 268 FDVLLDCAADGPDFLSEELDMLKNMKMAIYEERYKDAAMWRDKLTRLRKS 307
           F+V+LD A DGPD LSEEL +++NM +A  EERY DAAMWRD+L  L+ S
Sbjct: 182 FNVILDSAPDGPDPLSEELKLVRNMDLASKEERYIDAAMWRDRLKNLQNS 230

[Close]

BLAST of Carg19452 vs. TAIR 10
Match: AT1G19660.1 (Wound-responsive family protein )

HSP 1 Score: 129.4 bits (324), Expect = 5.0e-30
Identity = 100/304 (32.89%), Postives = 155/304 (50.99%), Query Frame = 0


Query: 9   SLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSI--LVSCHSSRDRSTH 68
           SLR  F G+    D   A+  +  LH             RR KSI  L S HS    ST 
Sbjct: 34  SLRKQFWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTA 93

Query: 69  SD-DDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSP 128
            + +++ +DY ++S L +  +           +D    Q+    N+P  G          
Sbjct: 94  ENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDG--RQLRCVHNNPQ-GGNLPNYAPHS 153

Query: 129 TVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTAR 188
            + LK+      LLPI+V E     L+ +   ++    P ++Q ++D++ K+GYEV   R
Sbjct: 154 AIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKDMVDKMGYEVRLVR 213

Query: 189 ITERVVNTYYARLFLRKDGE-NETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVS 248
           +T RV   Y+A L+L K G+ ++ +S D RPSDA+NIA RCK+P+ V+K + Y D +RV 
Sbjct: 214 VTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV- 273

Query: 249 FGFGRVRKRKSCFDVLLDCAADGPD----FLSEELDMLKNMKMAIYEERYKDAAMWRDKL 305
              G++ K+    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL
Sbjct: 274 IDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKL 320

[Close]

BLAST of Carg19452 vs. TAIR 10
Match: AT1G19660.2 (Wound-responsive family protein )

HSP 1 Score: 129.4 bits (324), Expect = 5.0e-30
Identity = 100/304 (32.89%), Postives = 155/304 (50.99%), Query Frame = 0


Query: 9   SLRTLFPGSAIAMDHPSASRPSSSLHFSVLRSRPNAVSSRRPKSI--LVSCHSSRDRSTH 68
           SLR  F G+    D   A+  +  LH             RR KSI  L S HS    ST 
Sbjct: 34  SLRKQFWGNQTKNDKSQAATVNLRLHL------------RRYKSIKCLFSSHSDGTGSTA 93

Query: 69  SD-DDSHQDYFEASFLVSETIYHYRMWKKRFQEDANFNQIESRVNSPSLGLGFFRRFRSP 128
            + +++ +DY ++S L +  +           +D    Q+    N+P  G          
Sbjct: 94  ENFNENDEDYVKSSVLEAVEVKSGPDGFMVKMKDG--RQLRCVHNNPQ-GGNLPNYAPHS 153

Query: 129 TVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTAR 188
            + LK+      LLPI+V E     L+ +   ++    P ++Q ++D++ K+GYEV   R
Sbjct: 154 AIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKDMVDKMGYEVRLVR 213

Query: 189 ITERVVNTYYARLFLRKDGE-NETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRVS 248
           +T RV   Y+A L+L K G+ ++ +S D RPSDA+NIA RCK+P+ V+K + Y D +RV 
Sbjct: 214 VTTRVHEAYFAELYLSKVGDKSDCVSFDLRPSDAINIAVRCKVPIQVNKYLAYSDGMRV- 273

Query: 249 FGFGRVRKRKSCFDVLLDCAADGPD----FLSEELDMLKNMKMAIYEERYKDAAMWRDKL 305
              G++ K+    D LL    D P+    F ++E D+++NM  A+ EERY +AA WRDKL
Sbjct: 274 IDSGKLSKQTPASDGLLFTELDRPNGQPCFDTKEFDLVRNMMQAVDEERYDEAAEWRDKL 320

[Close]

BLAST of Carg19452 vs. TAIR 10
Match: AT1G75380.1 (bifunctional nuclease in basal defense response 1 )

HSP 1 Score: 124.8 bits (312), Expect = 1.2e-28
Identity = 72/185 (38.92%), Postives = 108/185 (58.38%), Query Frame = 0


Query: 125 PTVFLKISCNGDFLLPIVVGEYAIEKLIDSHLGIENGEAPDLFQFIQDLMVKVGYEVTTA 184
           P + LK+      LLPI+V E     L+ +   ++    P ++Q +++++ K+GYEV   
Sbjct: 134 PAIVLKMEDGTGLLLPIIVLEMPSVLLMAAMTNVQIAR-PTMYQVVKEMVDKMGYEVRLV 193

Query: 185 RITERVVNTYYARLFLRKDGE-NETLSVDARPSDALNIAYRCKIPVLVSKQIVYEDAIRV 244
           R+T+RV   Y+A+LFL K G  +E +S D RPSDA+NIA RCKIP+ V+K + Y D +RV
Sbjct: 194 RVTKRVHEAYFAQLFLSKVGNASECVSFDLRPSDAINIAVRCKIPIQVNKYLAYSDGMRV 253

Query: 245 SFGFGRVRKRKSCFDVLLDCAADGPD----FLSEELDMLKNMKMAIYEERYKDAAMWRDK 304
               G++       D LL    D P+      ++E ++L  M  A+ EERY +AA WRDK
Sbjct: 254 -IESGKISTPAPASDGLLFTEQDRPNGQACLDTKEFNILSKMMQAVDEERYDEAAEWRDK 313

The following BLAST results are available for this feature:

BLAST of Carg19452 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7015839.1	1.7e-173	100.00	Bifunctional nuclease 2 [Cucurbita argyrosperma subsp. argyrosperma]	[more]
XP_022923551.1	2.4e-172	99.35	bifunctional nuclease 2-like [Cucurbita moschata] >XP_022923553.1 bifunctional n...	[more]
XP_022965292.1	1.0e-170	98.06	bifunctional nuclease 2-like [Cucurbita maxima] >XP_022965293.1 bifunctional nuc...	[more]
XP_023553147.1	8.7e-170	97.42	bifunctional nuclease 2-like isoform X1 [Cucurbita pepo subsp. pepo] >XP_0235531...	[more]
KAG6577800.1	6.7e-162	100.00	Bifunctional nuclease 2, partial [Cucurbita argyrosperma subsp. sororia]	[more]

BLAST of Carg19452 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q93VH2	7.0e-29	32.89	Bifunctional nuclease 2 OS=Arabidopsis thaliana OX=3702 GN=BBD2 PE=2 SV=1	[more]
Q6YZM6	4.5e-28	37.81	Bifunctional nuclease 2 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD2 PE=2 SV...	[more]
Q9FWS6	1.7e-27	38.92	Bifunctional nuclease 1 OS=Arabidopsis thaliana OX=3702 GN=BBD1 PE=2 SV=1	[more]
Q5N8J3	1.1e-26	38.46	Bifunctional nuclease 1 OS=Oryza sativa subsp. japonica OX=39947 GN=BBD1 PE=2 SV...	[more]
Q09LL3	1.1e-26	38.46	Bifunctional nuclease OS=Oryza minuta OX=63629 GN=BBD PE=2 SV=1	[more]

BLAST of Carg19452 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1E735	1.2e-172	99.35	bifunctional nuclease 2-like OS=Cucurbita moschata OX=3662 GN=LOC111431208 PE=3 ...	[more]
A0A6J1HJX9	5.0e-171	98.06	bifunctional nuclease 2-like OS=Cucurbita maxima OX=3661 GN=LOC111465202 PE=3 SV...	[more]
A0A0A0L3L8	2.0e-156	90.38	BFN domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_3G002490 PE=3 SV...	[more]
A0A1S3BJU6	7.7e-156	90.06	bifunctional nuclease 2 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103490665 PE=3 ...	[more]
A0A6J1CVQ2	8.8e-144	83.28	bifunctional nuclease 2 isoform X1 OS=Momordica charantia OX=3673 GN=LOC11101482...	[more]

BLAST of Carg19452 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G66050.1	3.1e-64	43.65	Wound-responsive family protein	[more]
AT5G66050.2	1.7e-59	48.70	Wound-responsive family protein	[more]
AT1G19660.1	5.0e-30	32.89	Wound-responsive family protein	[more]
AT1G19660.2	5.0e-30	32.89	Wound-responsive family protein	[more]
AT1G75380.1	1.2e-28	38.92	bifunctional nuclease in basal defense response 1	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036104	Bifunctional nuclease superfamily	GENE3D	3.10.690.10	Bifunctional nuclease domain	coord: 109..245 e-value: 5.5E-29 score: 102.6
IPR036104	Bifunctional nuclease superfamily	SUPERFAMILY	103256	Hypothetical protein TM0160	coord: 115..244
IPR003729	Bifunctional nuclease domain	PFAM	PF02577	DUF151	coord: 136..243 e-value: 3.0E-17 score: 62.4
IPR003729	Bifunctional nuclease domain	PROSITE	PS51658	BFN	coord: 106..243 score: 27.874271
None	No IPR available	PANTHER	PTHR15160:SF1	VON HIPPEL-LINDAU DISEASE TUMOR SUPPRESSOR-RELATED	coord: 33..303
None	No IPR available	PANTHER	PTHR15160	VON HIPPEL-LINDAU PROTEIN	coord: 33..303

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19452-RA	Carg19452-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0090305	nucleic acid phosphodiester bond hydrolysis
biological_process	GO:0016567	protein ubiquitination
cellular_component	GO:0005634	nucleus
cellular_component	GO:0030891	VCB complex
molecular_function	GO:0004518	nuclease activity