Carg19447 (gene) Silver-seed gourd (SMH-JMG-627) v2

BLAST of Carg19447 vs. NCBI nr
Match: KAG7015844.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1075.5 bits (2780), Expect = 0.0e+00
Identity = 534/534 (100.00%), Postives = 534/534 (100.00%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI
Sbjct: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 534

BLAST of Carg19447 vs. NCBI nr
Match: KAG6577804.1 (putative acyl-activating enzyme 1, peroxisomal, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1069.7 bits (2765), Expect = 8.2e-309
Identity = 531/534 (99.44%), Postives = 531/534 (99.44%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF
Sbjct: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPTVLKSLSQTYGNSIQFPALVLIP HPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD
Sbjct: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPTHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR
Sbjct: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEE VQFEDLFTS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEEIVQFEDLFTS 360

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH
Sbjct: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVRANGEA VSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI
Sbjct: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 534

BLAST of Carg19447 vs. NCBI nr
Match: XP_022923197.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita moschata])

HSP 1 Score: 1051.6 bits (2718), Expect = 2.3e-303
Identity = 519/534 (97.19%), Postives = 526/534 (98.50%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPFSPSPANF+PLSPVTF K++AAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           H+SPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPTVL+SLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVT DAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPW SRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDL TS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVRANGE  VSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 528

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIV+FCRTHLPEFMIPERVVFGDLPMNSVGKVQKFI REKAK LNTNSTI
Sbjct: 529 ASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNSTI 582

BLAST of Carg19447 vs. NCBI nr
Match: XP_023552791.1 (probable acyl-activating enzyme 2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1029.2 bits (2660), Expect = 1.2e-296
Identity = 507/534 (94.94%), Postives = 519/534 (97.19%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPFSP PANF+PLSP+TFF ++AAVY ARPS+IYG+RVFTWSETYGR LHLASALVHHF
Sbjct: 1   MDPFSPFPANFVPLSPLTFFNKSAAVYGARPSLIYGARVFTWSETYGRCLHLASALVHHF 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQL PKLIFLDS
Sbjct: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLDPKLIFLDS 120

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPTVLKSLSQTYGNS+QFPALVLIPAHPDTP SEFLDYNRVLGMRFDDFTPRTNAELD
Sbjct: 121 DFLPTVLKSLSQTYGNSVQFPALVLIPAHPDTPLSEFLDYNRVLGMRFDDFTPRTNAELD 180

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVT + IFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPW SRR
Sbjct: 241 IWAMAALGGCNICLRNVTTNVIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 300

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF EETVQFEDL TS
Sbjct: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFGEETVQFEDLITS 360

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH
Sbjct: 361 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVRANGEA VSTV+VEAVLMSHPSV EAAV+GERLCGFVKLKNGSR
Sbjct: 421 KSGRIEMKDRAKDIVVRANGEAAVSTVEVEAVLMSHPSVVEAAVIGERLCGFVKLKNGSR 480

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIVEFCR HLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAK LNTN+TI
Sbjct: 481 ASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKALNTNNTI 534

BLAST of Carg19447 vs. NCBI nr
Match: XP_023007650.1 (probable acyl-activating enzyme 1, peroxisomal [Cucurbita maxima])

HSP 1 Score: 1011.5 bits (2614), Expect = 2.6e-291
Identity = 500/534 (93.63%), Postives = 512/534 (95.88%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPF PSPANF+PLSP+ FFKRAAAVY  RPSVIYGSRVFTWSETYGR LHLASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPK+IFLDS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPT+L+SLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVL MRFD+FTPRTN+ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVT DAIFTNIELHKVTLLCGPSTLLKMIYESSSFNN KP F RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPI+EILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDL  S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDP SMESVLGDGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDVGV+H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVR NGE  VSTVQVEAVLMSHP VAEAAV+GERLCG VKLKNGSR
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGERLCGLVKLKNGSR 521

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIVEFCR HLPEFMIPERVVFGDLPMNSVGKVQKFI REKAK LNTN+TI
Sbjct: 522 ASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNTI 575

BLAST of Carg19447 vs. ExPASy Swiss-Prot
Match: Q9SEY5 (Isovalerate--CoA ligase AAE2 OS=Arabidopsis thaliana OX=3702 GN=AAE2 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 1.1e-130
Identity = 265/562 (47.15%), Postives = 356/562 (63.35%), Query Frame = 0

Query: 7   SPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGD 66
           SPANF PLSP+TF +R+A VY  R S+++GS   TW +TY R L LASAL  +  IS GD
Sbjct: 54  SPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGISRGD 113

Query: 67  VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 126
           VVAA+APNVP ++ELHFAVPMAG I+ PLNT+LD  TLS+LL     K++F+D   L   
Sbjct: 114 VVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIA 173

Query: 127 -----LKSLSQTYGNSIQFPALVLIPAHPDTPPSE-------------FLDYNRVL---G 186
                L + S     S++   LVLI    D   S+               +Y  +L    
Sbjct: 174 HGALDLLAKSDKTRKSLK---LVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGD 233

Query: 187 MRFDDFTPRTNAELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPV 246
             F+   PR   E D ISIN TSG+T   KGVVYSHR AYLNSLAT+F   + + +  PV
Sbjct: 234 SEFEIIKPR--CEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVF---LHQMSVYPV 293

Query: 247 FLWTVDMFRCNGWCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIY 306
           +LWTV MF CNGWC +W +AA GG NICLR V+   IF NI +HKVT + G  T+L MI 
Sbjct: 294 YLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIV 353

Query: 307 ESSSFNNFKPWFSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKP 366
            + +    KP    RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WKP
Sbjct: 354 -NYTVTEHKP-LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKP 413

Query: 367 NFDEETVQFEDLFTSLE---------IDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYY 426
            +D  +++      + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY+
Sbjct: 414 EWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYF 473

Query: 427 KNLKATREAFSGDWYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSH 486
           K+++ATR+AF GDW+ +GD+ V++  G IE+KDR KD+++  +G   +S+V+VE VL SH
Sbjct: 474 KDIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERVLCSH 533

Query: 487 PSVAEAAVV-------GERLCGFVKLKNG-SRASAEEIVEFCRTHLPEFMIPERVVFGDL 531
            +V EAAVV       G+  CGFVKLK G      EEI+ FCR HLP +M P+ +VFGD+
Sbjct: 534 QAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDI 593

BLAST of Carg19447 vs. ExPASy Swiss-Prot
Match: M4IS92 (Probable CoA ligase CCL13 OS=Humulus lupulus OX=3486 GN=CCL13 PE=2 SV=1)

HSP 1 Score: 466.8 bits (1200), Expect = 3.2e-130
Identity = 257/543 (47.33%), Postives = 342/543 (62.98%), Query Frame = 0

Query: 9   ANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGDVV 68
           AN +PLSP+TF +R++  Y    S++YGS  +TW++T+ R L LASAL  HF ISPGDVV
Sbjct: 37  ANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHFGISPGDVV 96

Query: 69  AAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLK 128
           A  + N+PE+YELHFAVPMAG I+  LN + D+  +S LL     KLIF++   L T   
Sbjct: 97  ATFSYNIPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARA 156

Query: 129 SLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDF-TPRTNAELDAISINCT 188
           +L       I+ P LVL+        S +  YN +L    DDF   R   E D ISIN T
Sbjct: 157 ALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNECDPISINYT 216

Query: 189 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 248
           SG+T   K VVYSHR AYLNS+AT+   G+    +  V+LW+V MF CNGWCF W  AA 
Sbjct: 217 SGTTARPKAVVYSHRGAYLNSIATVLLHGM---GTRSVYLWSVPMFHCNGWCFPWGAAAQ 276

Query: 249 GGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAG 308
           G  NIC+R V+  AIF NI LHKVT      T+L MI  S   N   P    +V+++  G
Sbjct: 277 GATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTP-LPHKVEVMTGG 336

Query: 309 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQFE-----DLF 368
           + P  +++ ++ E+GF +++ YG+TE  GPA     KP +D    EE    +     +  
Sbjct: 337 SPPPPKVIARMEEMGFQVNHIYGLTETHGPATNCVCKPEWDALQPEERYALKARQGLNHL 396

Query: 369 TSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGV 428
              E+DV+DP+SMESV  DG T+GEVM RGNT+MSGY+K+LKAT EAF G W+RTGD+GV
Sbjct: 397 AMEEMDVRDPVSMESVRADGTTIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRTGDLGV 456

Query: 429 RHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCG 488
           +H+ G I++KDR KD+V+  +G   VSTV+VE VL SH +V EAAVV       GE  C 
Sbjct: 457 KHEDGYIQLKDRKKDVVI--SGGENVSTVEVETVLYSHEAVLEAAVVARPDKLWGETPCA 516

Query: 489 FVKLKNG--SRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELN 533
           FV LK G  +  SA++I++FCR  LP +M P+ VVF +LP  S GK+QK+I++EKAK + 
Sbjct: 517 FVTLKEGFDNGVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAKAMG 573

BLAST of Carg19447 vs. ExPASy Swiss-Prot
Match: F4HUK6 (Butanoate--CoA ligase AAE1 OS=Arabidopsis thaliana OX=3702 GN=AAE1 PE=1 SV=1)

HSP 1 Score: 463.8 bits (1192), Expect = 2.7e-129
Identity = 256/546 (46.89%), Postives = 344/546 (63.00%), Query Frame = 0

Query: 7   SPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGD 66
           SPAN++PL+P++F  R+A VYA R S++YGS  +TW +T  R + +ASAL     IS GD
Sbjct: 9   SPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQLGISTGD 68

Query: 67  VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 126
           VV+ +APNVP + ELHF VPMAGA++  LN + D+  +++LL+    K+IF D  FL   
Sbjct: 69  VVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIA 128

Query: 127 LKSLSQTYGNSIQFPALVLIPAHPDTP-------PSEFLDYNRVLGMRFDDF-TPRTNAE 186
             +         + P LVLIP  P T          E ++Y  V+ M   DF   R   E
Sbjct: 129 EGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDE 188

Query: 187 LDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGW 246
            DAIS+N TSG+T   KGVVYSHR AYLNSLA +    + +  SSP +LWT  MF CNGW
Sbjct: 189 CDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVL---LNEMHSSPTYLWTNPMFHCNGW 248

Query: 247 CFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFS 306
           C +W + A+GG NICLRNVTA AIF NI  HKVT + G  T+L MI  +   +  KP   
Sbjct: 249 CLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPE-SEQKP-LP 308

Query: 307 RRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQF 366
            +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +D    EE  + 
Sbjct: 309 GKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKM 368

Query: 367 E-----DLFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD 426
           +     +     EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +AT+EAF G 
Sbjct: 369 KARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGG 428

Query: 427 WYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV---- 486
           W+ +GD+GV+H  G IE+KDR+KDI++  +G   +S+++VE+ L +HP V EAAVV    
Sbjct: 429 WFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEAAVVARPD 488

Query: 487 ---GERLCGFVKLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVR 529
              GE  C FVKLK+GS+ASAEE++ +CR  LP +M P  +VF DLP  S GKVQKF++R
Sbjct: 489 EYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLR 545

BLAST of Carg19447 vs. ExPASy Swiss-Prot
Match: M4IRL4 (Isovalerate--CoA ligase CCL2 OS=Humulus lupulus OX=3486 GN=CCL2 PE=1 SV=1)

HSP 1 Score: 459.1 bits (1180), Expect = 6.6e-128
Identity = 252/543 (46.41%), Postives = 341/543 (62.80%), Query Frame = 0

Query: 9   ANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGDVV 68
           AN +PLSP+TF +R++  Y    S++YGS  +TW++T+ R L LASAL  H  ISPGDVV
Sbjct: 37  ANHVPLSPITFLERSSKAYRDNTSLVYGSVRYTWAQTHHRCLKLASALTTHLGISPGDVV 96

Query: 69  AAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLK 128
           A  + N+PE+YELHFAVPMAG I+  LN + D+  +S LL     KLIF++   L T   
Sbjct: 97  ATFSYNLPEIYELHFAVPMAGGILCTLNARNDSAMVSTLLAHSEAKLIFVEPQLLETARA 156

Query: 129 SLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDF-TPRTNAELDAISINCT 188
           +L       I+ P LVL+        S +  YN +L    DDF   R   E D ISIN T
Sbjct: 157 ALDLLAQKDIKPPTLVLLTDSESFTSSSYDHYNHLLANGSDDFEIRRPKNEWDPISINYT 216

Query: 189 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 248
           SG+T   K VVYSHR AYLNS+AT+   G+    ++ V+LW+V MF CNGWCF W  AA 
Sbjct: 217 SGTTARPKAVVYSHRGAYLNSIATVLLHGM---GTTSVYLWSVPMFHCNGWCFPWGAAAQ 276

Query: 249 GGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAG 308
           G  NIC+R V+  AIF NI LHKVT      T+L MI  S   N   P    +V+++  G
Sbjct: 277 GATNICIRKVSPKAIFDNIHLHKVTHFGAAPTVLNMIVNSPEGNLHTP-LPHKVEVMTGG 336

Query: 309 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQFE-----DLF 368
           + P  +++ ++ E+GF +++ YG+TE  GPA     KP +D    EE    +     +  
Sbjct: 337 SPPPPKVIARMEEMGFQVNHIYGLTETCGPAANCVCKPEWDALQPEERYALKARQGLNHL 396

Query: 369 TSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGV 428
              E+DV+DP++MESV  DG T+GEVM RGNT+MSGY+K+LKAT EAF G W+R+GD+GV
Sbjct: 397 AMEEMDVRDPVTMESVRADGATIGEVMFRGNTVMSGYFKDLKATEEAFEGGWFRSGDLGV 456

Query: 429 RHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCG 488
           +H+ G I++KDR KD+V+  +G   +STV+VE VL SH +V EAAVV       GE  C 
Sbjct: 457 KHEDGYIQLKDRKKDVVI--SGGENISTVEVETVLYSHEAVLEAAVVARPDKLWGETPCA 516

Query: 489 FVKLKNG--SRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELN 533
           FV LK G  +  SA++I++FCR  LP +M P+ VVF +LP  S GK+QK+I++EKA  + 
Sbjct: 517 FVTLKEGFDNDVSADQIIKFCRDRLPHYMAPKTVVFEELPKTSTGKIQKYILKEKAMAMG 573

BLAST of Carg19447 vs. ExPASy Swiss-Prot
Match: Q9C8D4 (Butyrate--CoA ligase AAE11, peroxisomal OS=Arabidopsis thaliana OX=3702 GN=AAE11 PE=1 SV=1)

HSP 1 Score: 400.2 bits (1027), Expect = 3.6e-110
Identity = 233/561 (41.53%), Postives = 347/561 (61.85%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MD      AN +PL+P+TF KRA+  Y  R S+IYG   FTW +TY R   LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           +I+  DVV+ +APNVP +YE+HF+VPM GA+++P+NT+LDA T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 DFLPTV--LKSLSQTYGNSIQFPALVLIPAHPDT--PPSEFLDYNRVLGMRFDDFTPRTN 180
           +F P +  +  L  TY  S   P ++LI     T  P S+ LDY  ++  R  + TP ++
Sbjct: 121 EFAPLIQEVLRLIPTY-QSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSS 180

Query: 181 A-------ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATI--FRSGICKNTSSPVFL 240
           A       E D IS+N TSG+T   KGVV SH+ AYL++L++I  +  GI      PV+L
Sbjct: 181 ASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI-----FPVYL 240

Query: 241 WTVDMFRCNGWCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYES 300
           WT+ MF CNGW   W++AA GG N+C+R+VTA  I+ NIELH VT +    T+ + + E 
Sbjct: 241 WTLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEG 300

Query: 301 SSFNNFKPWFSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF 360
           S  +      S  V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+  +
Sbjct: 301 SRTDQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEW 360

Query: 361 DE--ETVQFE-------DLFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKN 420
           ++  E  Q E          T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN
Sbjct: 361 NKLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKN 420

Query: 421 LKATREAFSGDWYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPS 480
            KAT EAF   W  TGD+GV H  G +E+KDR+KDI++  +G   +S+++VE VL  +  
Sbjct: 421 PKATSEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQE 480

Query: 481 VAEAAVV-------GERLCGFVKLKNGSR---ASAEEIVEFCRTHLPEFMIPERVV-FGD 529
           V EAAVV       GE  C FV LK G      S  +++++CR ++P FM P++VV F +
Sbjct: 481 VLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQE 540

BLAST of Carg19447 vs. ExPASy TrEMBL
Match: A0A6J1E5I3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita moschata OX=3662 GN=LOC111430963 PE=4 SV=1)

HSP 1 Score: 1051.6 bits (2718), Expect = 1.1e-303
Identity = 519/534 (97.19%), Postives = 526/534 (98.50%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPFSPSPANF+PLSPVTF K++AAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF
Sbjct: 49  MDPFSPSPANFVPLSPVTFLKKSAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 108

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           H+SPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS
Sbjct: 109 HVSPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 168

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPTVL+SLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD
Sbjct: 169 DFLPTVLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 228

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 229 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 288

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVT DAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPW SRR
Sbjct: 289 IWAMAALGGCNICLRNVTTDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWLSRR 348

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDL TS
Sbjct: 349 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLITS 408

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH
Sbjct: 409 LEIDVKDPLSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 468

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVRANGE  VSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR
Sbjct: 469 KSGRIEMKDRAKDIVVRANGEVAVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 528

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIV+FCRTHLPEFMIPERVVFGDLPMNSVGKVQKFI REKAK LNTNSTI
Sbjct: 529 ASAEEIVKFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNSTI 582

BLAST of Carg19447 vs. ExPASy TrEMBL
Match: A0A6J1L3K3 (probable acyl-activating enzyme 1, peroxisomal OS=Cucurbita maxima OX=3661 GN=LOC111500216 PE=4 SV=1)

HSP 1 Score: 1011.5 bits (2614), Expect = 1.3e-291
Identity = 500/534 (93.63%), Postives = 512/534 (95.88%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MDPF PSPANF+PLSP+ FFKRAAAVY  RPSVIYGSRVFTWSETYGR LHLASALVHHF
Sbjct: 42  MDPFFPSPANFVPLSPLAFFKRAAAVYGGRPSVIYGSRVFTWSETYGRCLHLASALVHHF 101

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPK+IFLDS
Sbjct: 102 HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKVIFLDS 161

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLGMRFDDFTPRTNAELD 180
           DFLPT+L+SLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVL MRFD+FTPRTN+ELD
Sbjct: 162 DFLPTLLQSLSQTYGNSIQFPALVLIPAHPDTPPSEFLDYNRVLEMRFDEFTPRTNSELD 221

Query: 181 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 240
           AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF
Sbjct: 222 AISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCF 281

Query: 241 IWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRR 300
           IWAMAALGGCNICLRNVT DAIFTNIELHKVTLLCGPSTLLKMIYESSSFNN KP F RR
Sbjct: 282 IWAMAALGGCNICLRNVTPDAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNCKPGFLRR 341

Query: 301 VDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTS 360
           VDLIVAGALPI+EILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDL  S
Sbjct: 342 VDLIVAGALPIDEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLIAS 401

Query: 361 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 420
           LEIDVKDP SMESVLGDGETLGEVMLRGN+LMSGYYKNLKAT EAFSGDWYRTGDVGV+H
Sbjct: 402 LEIDVKDPSSMESVLGDGETLGEVMLRGNSLMSGYYKNLKATGEAFSGDWYRTGDVGVKH 461

Query: 421 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGERLCGFVKLKNGSR 480
           KSGRIEMKDRAKDIVVR NGE  VSTVQVEAVLMSHP VAEAAV+GERLCG VKLKNGSR
Sbjct: 462 KSGRIEMKDRAKDIVVRENGEGAVSTVQVEAVLMSHPGVAEAAVIGERLCGLVKLKNGSR 521

Query: 481 ASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNSTI 535
           ASAEEIVEFCR HLPEFMIPERVVFGDLPMNSVGKVQKFI REKAK LNTN+TI
Sbjct: 522 ASAEEIVEFCRAHLPEFMIPERVVFGDLPMNSVGKVQKFIAREKAKALNTNNTI 575

BLAST of Carg19447 vs. ExPASy TrEMBL
Match: A0A1S3BKP8 (probable acyl-activating enzyme 2 OS=Cucumis melo OX=3656 GN=LOC103490658 PE=4 SV=1)

HSP 1 Score: 834.7 bits (2155), Expect = 2.1e-238
Identity = 426/543 (78.45%), Postives = 471/543 (86.74%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           M+ F   P NF PLSPV F KRA+ +Y  RPS++YGSRVFTWS+TYGR L +ASALVHHF
Sbjct: 1   MEGFHHCPPNFAPLSPVPFLKRASTLYGCRPSLLYGSRVFTWSQTYGRCLSIASALVHHF 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           H+SP D+V AMAPN+PELYELHFAVPMAG IIS LNTKLD+PTLSLLLQQL+PK+IFLDS
Sbjct: 61  HLSPADLVVAMAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDS 120

Query: 121 DFLPTVLKSLSQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLGMRF--DDFTPRTNA 180
            FLP +LKSL      SI+FPAL+LIP+ P+T  PS+FLDYN++L MR   D+FTPR NA
Sbjct: 121 QFLPILLKSLENI---SIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNA 180

Query: 181 ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNG 240
           ELD ISIN TSGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNG
Sbjct: 181 ELDPISINYTSGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNG 240

Query: 241 WCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWF 300
           WCFIW MAALGGCNICLR VTADAIFTN+ELH+VTLLCGPSTLLKMIYESSS N      
Sbjct: 241 WCFIWVMAALGGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRL 300

Query: 301 SRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF-DEETVQFED 360
           SRRVDLIVAGALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP+F D++ VQF+D
Sbjct: 301 SRRVDLIVAGALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDD 360

Query: 361 -LFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTG 420
            L TSLEIDVKDP+SMESVLGDGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTG
Sbjct: 361 HLITSLEIDVKDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTG 420

Query: 421 DVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGER-LCGFV 480
           DVGVRHKSGRIEMKDRAKDIVVR +GE  VSTV+VE VLMSHP+VAE AVVGER L GFV
Sbjct: 421 DVGVRHKSGRIEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFV 480

Query: 481 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKEL--NTN 535
           KLKNGS+ + +EIVEFCR HLPEFM+P+ +VFGDLPMNS GKVQKF++REKAK L    N
Sbjct: 481 KLKNGSKENNDEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNN 540

BLAST of Carg19447 vs. ExPASy TrEMBL
Match: A0A0A0L3L0 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_3G002400 PE=4 SV=1)

HSP 1 Score: 757.7 bits (1955), Expect = 3.3e-215
Identity = 385/467 (82.44%), Postives = 415/467 (88.87%), Query Frame = 0

Query: 71  MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLKSL 130
           MAPN+PELYELHFAVPMAGAIIS LNTKLD+PTLSLLLQQL+PK+IF+DS FLP +LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGAIISALNTKLDSPTLSLLLQQLNPKVIFIDSQFLPILLKSL 60

Query: 131 SQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLGMRF--DDFTPRTNAELDAISINCT 190
                +SI+FPALVLIP+ PDTP PSEFLDYN+VL MRF  DDFTPR NAELD ISIN T
Sbjct: 61  E---NSSIKFPALVLIPSDPDTPLPSEFLDYNKVLAMRFGDDDFTPRPNAELDPISINYT 120

Query: 191 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 250
           SGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNGWCFIW MAAL
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180

Query: 251 GGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAG 310
           GGCNICLR VTADAIFTN+ELH+VTLLCGP TLLKMIYESSS N      SRRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPPTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240

Query: 311 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFDEETVQFEDLFTSLEIDVKD 370
           ALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP F+E+ VQFEDL TSLEIDVKD
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPTFEEDNVQFEDLITSLEIDVKD 300

Query: 371 PMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGDVGVRHKSGRIE 430
           P+SMESVLGDGETLGEVMLRGNTLMSGYYKN+KAT EAF G+ WYRTGDVGVRHKSGRIE
Sbjct: 301 PISMESVLGDGETLGEVMLRGNTLMSGYYKNMKATHEAFIGENWYRTGDVGVRHKSGRIE 360

Query: 431 MKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGER-LCGFVKLKNGSRASAEE 490
           MKDRAKDIVVR +GE  VSTV+VE VLMSHP+VAEAAVVGER L GFVKLKN S+ + +E
Sbjct: 361 MKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAEAAVVGERTLYGFVKLKNRSKENGDE 420

Query: 491 IVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKELNTNS 533
           IVEFCR HLPEFMIP+R+VFGDLPMNS GKVQKF +REK K L  N+
Sbjct: 421 IVEFCRMHLPEFMIPKRIVFGDLPMNSTGKVQKFALREKVKALLNNA 464

BLAST of Carg19447 vs. ExPASy TrEMBL
Match: A0A5A7UEI8 (Putative acyl-activating enzyme 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold139G00360 PE=4 SV=1)

HSP 1 Score: 737.6 bits (1903), Expect = 3.6e-209
Identity = 380/473 (80.34%), Postives = 416/473 (87.95%), Query Frame = 0

Query: 71  MAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLKSL 130
           MAPN+PELYELHFAVPMAG IIS LNTKLD+PTLSLLLQQL+PK+IFLDS FLP +LKSL
Sbjct: 1   MAPNIPELYELHFAVPMAGGIISALNTKLDSPTLSLLLQQLNPKVIFLDSQFLPILLKSL 60

Query: 131 SQTYGNSIQFPALVLIPAHPDTP-PSEFLDYNRVLGMRF--DDFTPRTNAELDAISINCT 190
                 SI+FPAL+LIP+ P+T  PS+FLDYN++L MR   D+FTPR NAELD ISIN T
Sbjct: 61  ENI---SIKFPALILIPSDPNTSLPSQFLDYNKILTMRLGVDNFTPRPNAELDPISINYT 120

Query: 191 SGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAAL 250
           SGSTGLHKGV+YSHRAAYLNSLATIFRS IC +TSSPVFLWTVDMFRCNGWCFIW MAAL
Sbjct: 121 SGSTGLHKGVIYSHRAAYLNSLATIFRSKICSSTSSPVFLWTVDMFRCNGWCFIWVMAAL 180

Query: 251 GGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAG 310
           GGCNICLR VTADAIFTN+ELH+VTLLCGPSTLLKMIYESSS N      SRRVDLIVAG
Sbjct: 181 GGCNICLRTVTADAIFTNVELHRVTLLCGPSTLLKMIYESSSNNCMPRRLSRRVDLIVAG 240

Query: 311 ALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF-DEETVQFED-LFTSLEIDV 370
           ALPI EILTKV ELGFNISYGYGMTEAMGPA+IRPWKP+F D++ VQF+D L TSLEIDV
Sbjct: 241 ALPIKEILTKVNELGFNISYGYGMTEAMGPAIIRPWKPSFEDQDNVQFDDHLITSLEIDV 300

Query: 371 KDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD-WYRTGDVGVRHKSGR 430
           KDP+SMESVLGDGETLGEVMLRGNTLMSGYYKNLKAT EAF G+ WYRTGDVGVRHKSGR
Sbjct: 301 KDPISMESVLGDGETLGEVMLRGNTLMSGYYKNLKATHEAFVGENWYRTGDVGVRHKSGR 360

Query: 431 IEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVVGER-LCGFVKLKNGSRASA 490
           IEMKDRAKDIVVR +GE  VSTV+VE VLMSHP+VAE AVVGER L GFVKLKNGS+ + 
Sbjct: 361 IEMKDRAKDIVVRTDGEGAVSTVEVEGVLMSHPNVAETAVVGERTLYGFVKLKNGSKENN 420

Query: 491 EEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKEL--NTNSTI 535
           +EIVEFCR HLPEFM+P+ +VFGDLPMNS GKVQKF++REKAK L    N+TI
Sbjct: 421 DEIVEFCRMHLPEFMVPKTIVFGDLPMNSTGKVQKFVLREKAKALLNGNNNTI 470

BLAST of Carg19447 vs. TAIR 10
Match: AT2G17650.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 468.4 bits (1204), Expect = 7.8e-132
Identity = 265/562 (47.15%), Postives = 356/562 (63.35%), Query Frame = 0

Query: 7   SPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGD 66
           SPANF PLSP+TF +R+A VY  R S+++GS   TW +TY R L LASAL  +  IS GD
Sbjct: 54  SPANFSPLSPITFLERSAKVYRDRTSLVFGSVKHTWFQTYQRCLRLASALT-NLGISRGD 113

Query: 67  VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 126
           VVAA+APNVP ++ELHFAVPMAG I+ PLNT+LD  TLS+LL     K++F+D   L   
Sbjct: 114 VVAALAPNVPAMHELHFAVPMAGLILCPLNTRLDPSTLSVLLAHSEAKILFVDHQLLEIA 173

Query: 127 -----LKSLSQTYGNSIQFPALVLIPAHPDTPPSE-------------FLDYNRVL---G 186
                L + S     S++   LVLI    D   S+               +Y  +L    
Sbjct: 174 HGALDLLAKSDKTRKSLK---LVLISQSNDDDDSDEDSSSTFASKYSFDYEYETLLKSGD 233

Query: 187 MRFDDFTPRTNAELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPV 246
             F+   PR   E D ISIN TSG+T   KGVVYSHR AYLNSLAT+F   + + +  PV
Sbjct: 234 SEFEIIKPR--CEWDPISINYTSGTTSRPKGVVYSHRGAYLNSLATVF---LHQMSVYPV 293

Query: 247 FLWTVDMFRCNGWCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIY 306
           +LWTV MF CNGWC +W +AA GG NICLR V+   IF NI +HKVT + G  T+L MI 
Sbjct: 294 YLWTVPMFHCNGWCLVWGVAAQGGTNICLRKVSPKMIFKNIAMHKVTHMGGAPTVLNMIV 353

Query: 307 ESSSFNNFKPWFSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKP 366
            + +    KP    RV+++  G+ P+ +IL K+ ELGFN+S+ YG+TE  GP     WKP
Sbjct: 354 -NYTVTEHKP-LPHRVEIMTGGSPPLPQILAKMEELGFNVSHLYGLTETYGPGTHCVWKP 413

Query: 367 NFDEETVQFEDLFTSLE---------IDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYY 426
            +D  +++      + +         +DVKDP++ME+V  DG T+GEVM RGNT+MSGY+
Sbjct: 414 EWDSLSLEERTKLKARQGVQHLGLEGLDVKDPLTMETVPDDGLTMGEVMFRGNTVMSGYF 473

Query: 427 KNLKATREAFSGDWYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSH 486
           K+++ATR+AF GDW+ +GD+ V++  G IE+KDR KD+++  +G   +S+V+VE VL SH
Sbjct: 474 KDIEATRKAFEGDWFHSGDLAVKYPDGYIEIKDRLKDVII--SGGENISSVEVERVLCSH 533

Query: 487 PSVAEAAVV-------GERLCGFVKLKNG-SRASAEEIVEFCRTHLPEFMIPERVVFGDL 531
            +V EAAVV       G+  CGFVKLK G      EEI+ FCR HLP +M P+ +VFGD+
Sbjct: 534 QAVLEAAVVARPDHHWGQTPCGFVKLKEGFDTIKPEEIIGFCRDHLPHYMAPKTIVFGDI 593

BLAST of Carg19447 vs. TAIR 10
Match: AT1G20560.1 (acyl activating enzyme 1 )

HSP 1 Score: 463.8 bits (1192), Expect = 1.9e-130
Identity = 256/546 (46.89%), Postives = 344/546 (63.00%), Query Frame = 0

Query: 7   SPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHFHISPGD 66
           SPAN++PL+P++F  R+A VYA R S++YGS  +TW +T  R + +ASAL     IS GD
Sbjct: 9   SPANYVPLTPISFLDRSAVVYADRVSIVYGSVKYTWRQTRDRCVRIASAL-SQLGISTGD 68

Query: 67  VVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTV 126
           VV+ +APNVP + ELHF VPMAGA++  LN + D+  +++LL+    K+IF D  FL   
Sbjct: 69  VVSVLAPNVPAMVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIA 128

Query: 127 LKSLSQTYGNSIQFPALVLIPAHPDTP-------PSEFLDYNRVLGMRFDDF-TPRTNAE 186
             +         + P LVLIP  P T          E ++Y  V+ M   DF   R   E
Sbjct: 129 EGACEILSNKGDKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDE 188

Query: 187 LDAISINCTSGSTGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGW 246
            DAIS+N TSG+T   KGVVYSHR AYLNSLA +    + +  SSP +LWT  MF CNGW
Sbjct: 189 CDAISVNYTSGTTSSPKGVVYSHRGAYLNSLAAVL---LNEMHSSPTYLWTNPMFHCNGW 248

Query: 247 CFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFS 306
           C +W + A+GG NICLRNVTA AIF NI  HKVT + G  T+L MI  +   +  KP   
Sbjct: 249 CLLWGVTAIGGTNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPE-SEQKP-LP 308

Query: 307 RRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQF 366
            +V  I   A P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +D    EE  + 
Sbjct: 309 GKVSFITGAAPPPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKM 368

Query: 367 E-----DLFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGD 426
           +     +     EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +AT+EAF G 
Sbjct: 369 KARQGVNHLGLEEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGG 428

Query: 427 WYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV---- 486
           W+ +GD+GV+H  G IE+KDR+KDI++  +G   +S+++VE+ L +HP V EAAVV    
Sbjct: 429 WFWSGDLGVKHPDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEAAVVARPD 488

Query: 487 ---GERLCGFVKLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVR 529
              GE  C FVKLK+GS+ASAEE++ +CR  LP +M P  +VF DLP  S GKVQKF++R
Sbjct: 489 EYWGETACAFVKLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLR 545

BLAST of Carg19447 vs. TAIR 10
Match: AT1G66120.1 (AMP-dependent synthetase and ligase family protein )

HSP 1 Score: 400.2 bits (1027), Expect = 2.6e-111
Identity = 233/561 (41.53%), Postives = 347/561 (61.85%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MD      AN +PL+P+TF KRA+  Y  R S+IYG   FTW +TY R   LA++L+   
Sbjct: 1   MDNLVLCEANNVPLTPITFLKRASECYPNRTSIIYGQTRFTWPQTYDRCCRLAASLL-SL 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           +I+  DVV+ +APNVP +YE+HF+VPM GA+++P+NT+LDA T++++L+   PK++F+D 
Sbjct: 61  NITRNDVVSILAPNVPAMYEMHFSVPMTGAVLNPINTRLDAKTIAIILRHAEPKILFVDY 120

Query: 121 DFLPTV--LKSLSQTYGNSIQFPALVLIPAHPDT--PPSEFLDYNRVLGMRFDDFTPRTN 180
           +F P +  +  L  TY  S   P ++LI     T  P S+ LDY  ++  R  + TP ++
Sbjct: 121 EFAPLIQEVLRLIPTY-QSQPHPRIILINEIDSTTKPFSKELDYEGLI--RKGEPTPSSS 180

Query: 181 A-------ELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATI--FRSGICKNTSSPVFL 240
           A       E D IS+N TSG+T   KGVV SH+ AYL++L++I  +  GI      PV+L
Sbjct: 181 ASMFRVHNEHDPISLNYTSGTTADPKGVVISHQGAYLSALSSIIGWEMGI-----FPVYL 240

Query: 241 WTVDMFRCNGWCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYES 300
           WT+ MF CNGW   W++AA GG N+C+R+VTA  I+ NIELH VT +    T+ + + E 
Sbjct: 241 WTLPMFHCNGWTHTWSVAARGGTNVCIRHVTAPEIYKNIELHGVTHMSCVPTVFRFLLEG 300

Query: 301 SSFNNFKPWFSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNF 360
           S  +      S  V ++  G+ P   ++ KV +LGF++ +GYG+TEA GP +   W+  +
Sbjct: 301 SRTDQSPK--SSPVQVLTGGSSPPAVLIKKVEQLGFHVMHGYGLTEATGPVLFCEWQDEW 360

Query: 361 DE--ETVQFE-------DLFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKN 420
           ++  E  Q E          T  ++DVK+  ++ESV  DG+T+GE++++G++LM GY KN
Sbjct: 361 NKLPEHQQIELQQRQGVRNLTLADVDVKNTKTLESVPRDGKTMGEIVIKGSSLMKGYLKN 420

Query: 421 LKATREAFSGDWYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPS 480
            KAT EAF   W  TGD+GV H  G +E+KDR+KDI++  +G   +S+++VE VL  +  
Sbjct: 421 PKATSEAFKHGWLNTGDIGVIHPDGYVEIKDRSKDIII--SGGENISSIEVEKVLYMYQE 480

Query: 481 VAEAAVV-------GERLCGFVKLKNGSR---ASAEEIVEFCRTHLPEFMIPERVV-FGD 529
           V EAAVV       GE  C FV LK G      S  +++++CR ++P FM P++VV F +
Sbjct: 481 VLEAAVVAMPHPLWGETPCAFVVLKKGEEGLVTSEGDLIKYCRENMPHFMCPKKVVFFQE 540

BLAST of Carg19447 vs. TAIR 10
Match: AT1G20560.2 (acyl activating enzyme 1 )

HSP 1 Score: 392.5 bits (1007), Expect = 5.4e-109
Identity = 219/475 (46.11%), Postives = 293/475 (61.68%), Query Frame = 0

Query: 78  LYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDSDFLPTVLKSLSQTYGNS 137
           + ELHF VPMAGA++  LN + D+  +++LL+    K+IF D  FL     +        
Sbjct: 1   MVELHFGVPMAGALLCTLNIRHDSSLVAVLLRHSGTKVIFADHQFLQIAEGACEILSNKG 60

Query: 138 IQFPALVLIPAHPDTP-------PSEFLDYNRVLGMRFDDF-TPRTNAELDAISINCTSG 197
            + P LVLIP  P T          E ++Y  V+ M   DF   R   E DAIS+N TSG
Sbjct: 61  DKVPILVLIP-EPLTQSVSRKKRSEEMMEYEDVVAMGKSDFEVIRPTDECDAISVNYTSG 120

Query: 198 STGLHKGVVYSHRAAYLNSLATIFRSGICKNTSSPVFLWTVDMFRCNGWCFIWAMAALGG 257
           +T   KGVVYSHR AYLNSLA +    + +  SSP +LWT  MF CNGWC +W + A+GG
Sbjct: 121 TTSSPKGVVYSHRGAYLNSLAAVL---LNEMHSSPTYLWTNPMFHCNGWCLLWGVTAIGG 180

Query: 258 CNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESSSFNNFKPWFSRRVDLIVAGAL 317
            NICLRNVTA AIF NI  HKVT + G  T+L MI  +   +  KP    +V  I   A 
Sbjct: 181 TNICLRNVTAKAIFDNISQHKVTHMGGAPTILNMIINAPE-SEQKP-LPGKVSFITGAAP 240

Query: 318 PINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD----EETVQFE-----DLFTS 377
           P   ++ K+ ELGF++ + YG+TE  GP  I  WKP +D    EE  + +     +    
Sbjct: 241 PPAHVIFKMEELGFSMFHSYGLTETYGPGTICTWKPEWDSLPREEQAKMKARQGVNHLGL 300

Query: 378 LEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNLKATREAFSGDWYRTGDVGVRH 437
            EI VKDP++M ++  DG T+GEV+ RGNT+M+GY KN +AT+EAF G W+ +GD+GV+H
Sbjct: 301 EEIQVKDPVTMRTLPADGVTMGEVVFRGNTVMNGYLKNPEATKEAFKGGWFWSGDLGVKH 360

Query: 438 KSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSVAEAAVV-------GERLCGFV 497
             G IE+KDR+KDI++  +G   +S+++VE+ L +HP V EAAVV       GE  C FV
Sbjct: 361 PDGYIELKDRSKDIII--SGGENISSIEVESTLFTHPCVLEAAVVARPDEYWGETACAFV 420

Query: 498 KLKNGSRASAEEIVEFCRTHLPEFMIPERVVFGDLPMNSVGKVQKFIVREKAKEL 529
           KLK+GS+ASAEE++ +CR  LP +M P  +VF DLP  S GKVQKF++R KAK L
Sbjct: 421 KLKDGSKASAEELISYCRDRLPHYMAPRSIVFEDLPKTSTGKVQKFVLRTKAKAL 467

BLAST of Carg19447 vs. TAIR 10
Match: AT1G65880.1 (benzoyloxyglucosinolate 1 )

HSP 1 Score: 382.5 bits (981), Expect = 5.6e-106
Identity = 227/568 (39.96%), Postives = 330/568 (58.10%), Query Frame = 0

Query: 1   MDPFSPSPANFLPLSPVTFFKRAAAVYAARPSVIYGSRVFTWSETYGRSLHLASALVHHF 60
           MD  +   AN +PL+P+TF KRA+  Y  R S+IYG   FTW +TY R   LA++L+   
Sbjct: 1   MDDLALCEANNVPLTPMTFLKRASECYPNRTSIIYGKTRFTWPQTYDRCCRLAASLI-SL 60

Query: 61  HISPGDVVAAMAPNVPELYELHFAVPMAGAIISPLNTKLDAPTLSLLLQQLHPKLIFLDS 120
           +IS  DVV+ MAPN P LYE+HFAVPMAGA+++P+NT+LDA +++ +L+   PK++FLD 
Sbjct: 61  NISKNDVVSVMAPNTPALYEMHFAVPMAGAVLNPINTRLDATSIAAILRHAKPKILFLDR 120

Query: 121 DFLPTVLKSLS--QTYGNSIQFPALVLIP-AHPDTPPSEFLDYNRVLGMRFDDFTP---- 180
            F     +SL    +  +++  P + +     P     E LDY  ++  +  + TP    
Sbjct: 121 SFEALARESLHLLSSEDSNLNLPVIFIHENDFPKRASFEELDYECLI--QRGEPTPSMVA 180

Query: 181 ---RTNAELDAISINCTSGSTGLHKGVVYSHRAAYLNSLATI--FRSGICKNTSSPVFLW 240
              R   E D IS+N TSG+T   KGVV SHR AYL +L+ I  +  G C     PV+LW
Sbjct: 181 RMFRIQDEHDPISLNYTSGTTADPKGVVISHRGAYLCTLSAIIGWEMGTC-----PVYLW 240

Query: 241 TVDMFRCNGWCFIWAMAALGGCNICLRNVTADAIFTNIELHKVTLLCGPSTLLKMIYESS 300
           T+ MF CNGW F W  AA GG ++C+R+VTA  I+ NIE+H VT +C   T+  ++ + +
Sbjct: 241 TLPMFHCNGWTFTWGTAARGGTSVCMRHVTAPEIYKNIEMHNVTHMCCVPTVFNILLKGN 300

Query: 301 SFNNFKPWFSRRVDLIVAGALPINEILTKVTELGFNISYGYGMTEAMGPAMIRPWKPNFD 360
           S  +  P  S  V ++  G+ P   ++ KV  LGF + + YG TEA GP +   W+  ++
Sbjct: 301 SL-DLSP-RSGPVHVLTGGSPPPAALVKKVQRLGFQVMHAYGQTEATGPILFCEWQDEWN 360

Query: 361 E--ETVQFE-------DLFTSLEIDVKDPMSMESVLGDGETLGEVMLRGNTLMSGYYKNL 420
              E  Q E        +    ++DVK+  + +S   DG+T+GE++++G+++M GY KN 
Sbjct: 361 RLPENQQMELKARQGISILGLADVDVKNKETQKSAPRDGKTMGEILIKGSSIMKGYLKNP 420

Query: 421 KATREAFSGDWYRTGDVGVRHKSGRIEMKDRAKDIVVRANGEATVSTVQVEAVLMSHPSV 480
           KAT EAF   W  TGDVGV H  G +E+KDR+KDI++  +G   +S+V+VE VL  +P V
Sbjct: 421 KATFEAFKHGWLNTGDVGVIHPDGHVEIKDRSKDIII--SGGENISSVEVENVLYKYPKV 480

Query: 481 AEAAVV-------GERLCGFVKLKNGSRASAEE-----------IVEFCRTHLPEFMIPE 529
            E AVV       GE  C FV L+       E+           ++E+CR +LP FM P 
Sbjct: 481 LETAVVAMPHPTWGETPCAFVVLEKSETTIKEDRVDKFQTRERNLIEYCRENLPHFMCPR 540