Carg19383 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19383
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	30S ribosomal protein S31, mitochondrial-like
Location	Carg_Chr19: 6941279 .. 6941539 (-)
RNA-Seq Expression	Carg19383
Synteny	Carg19383

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCAGTGGTGCGGCGCGTTAGCTAAGCGAGTGATGGCGGCGGAGCGAGGGTCTCCTTCACTGACGTCGTCCATGGCGGTGGAAGGACTGGTGCCGATCCTTTGTGGACGAGGTGACAAGAAGACGAAGAAGGGGAAAATATTCAAAGGCTCGTACGGCAACGCTAGGCCGAAGAAGGAGAAGAAGATTCAGCGAATTAAGGATAAAATCGAGGTTCCTAGTTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATCTGA

mRNA sequence

ATGGCGCAGTGGTGCGGCGCGTTAGCTAAGCGAGTGATGGCGGCGGAGCGAGGGTCTCCTTCACTGACGTCGTCCATGGCGGTGGAAGGACTGGTGCCGATCCTTTGTGGACGAGGTGACAAGAAGACGAAGAAGGGGAAAATATTCAAAGGCTCGTACGGCAACGCTAGGCCGAAGAAGGAGAAGAAGATTCAGCGAATTAAGGATAAAATCGAGGTTCCTAGTTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATCTGA

Coding sequence (CDS)

ATGGCGCAGTGGTGCGGCGCGTTAGCTAAGCGAGTGATGGCGGCGGAGCGAGGGTCTCCTTCACTGACGTCGTCCATGGCGGTGGAAGGACTGGTGCCGATCCTTTGTGGACGAGGTGACAAGAAGACGAAGAAGGGGAAAATATTCAAAGGCTCGTACGGCAACGCTAGGCCGAAGAAGGAGAAGAAGATTCAGCGAATTAAGGATAAAATCGAGGTTCCTAGTTCTACTCCTTGGCCTCTTCCTTTCAAGCTCATCTGA

Protein sequence

MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKKEKKIQRIKDKIEVPSSTPWPLPFKLI

Homology

[Close]

BLAST of Carg19383 vs. NCBI nr
Match: KAG6572068.1 (30S ribosomal protein S31, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011734.1 30S ribosomal protein S31, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 173.7 bits (439), Expect = 6.8e-40
Identity = 86/86 (100.00%), Postives = 86/86 (100.00%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. NCBI nr
Match: XP_023554667.1 (30S ribosomal protein S31, mitochondrial-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 171.4 bits (433), Expect = 3.4e-39
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGALAKRVMAAERGSP LTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 1  MAQWCGALAKRVMAAERGSPLLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. NCBI nr
Match: XP_022952601.1 (30S ribosomal protein S31, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 170.6 bits (431), Expect = 5.8e-39
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGALAKRVMAAER SPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 1  MAQWCGALAKRVMAAERRSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. NCBI nr
Match: XP_022969451.1 (30S ribosomal protein S31, mitochondrial-like [Cucurbita maxima])

HSP 1 Score: 167.9 bits (424), Expect = 3.7e-38
Identity = 84/86 (97.67%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGALAKRVMAAER SPS TSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 1  MAQWCGALAKRVMAAERRSPSPTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. NCBI nr
Match: XP_022933003.1 (30S ribosomal protein S31, mitochondrial [Cucurbita moschata] >XP_023529502.1 30S ribosomal protein S31, mitochondrial isoform X1 [Cucurbita pepo subsp. pepo] >XP_023529503.1 30S ribosomal protein S31, mitochondrial isoform X2 [Cucurbita pepo subsp. pepo] >KAG6588415.1 30S ribosomal protein S31, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia] >KAG7022258.1 30S ribosomal protein S31, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 163.7 bits (413), Expect = 7.1e-37
Identity = 81/86 (94.19%), Postives = 83/86 (96.51%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          M QWCGA+A+ VMAAER SPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 3  MMQWCGAVARGVMAAERRSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 62

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 63 EKKIQRIKDKIEVPSSTPWPLPFKLI 88

[Close]

BLAST of Carg19383 vs. ExPASy Swiss-Prot
Match: Q9SJU8 (30S ribosomal protein S31, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g21290 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 2.4e-24
Identity = 56/94 (59.57%), Postives = 66/94 (70.21%), Query Frame = 0


Query: 3  QWCGALAKRVMAAERGSPSLTSSMAVEGLVPI----------LCGRGDKKTKKGKIFKGS 62
          QWCGA+ +R+M  +R S +L  S     L P           LCGRGDKKTKKGK FKGS
Sbjct: 5  QWCGAMTRRIMMTQRTSAALNCSARYSSLSPACAAPAVSEMDLCGRGDKKTKKGKRFKGS 64

Query: 63 YGNARPKKEKKIQRIKDKIEVPSSTPWPLPFKLI 87
          YGN+R KK+K I+RIKDK+E+P STPWPLPFKLI
Sbjct: 65 YGNSRGKKQKMIERIKDKLELPRSTPWPLPFKLI 98

[Close]

BLAST of Carg19383 vs. ExPASy Swiss-Prot
Match: P47909 (30S ribosomal protein S31, mitochondrial OS=Oryza sativa subsp. japonica OX=39947 GN=Os09g0528100 PE=3 SV=2)

HSP 1 Score: 99.0 bits (245), Expect = 2.8e-20
Identity = 44/53 (83.02%), Postives = 50/53 (94.34%), Query Frame = 0


Query: 34 ILCGRGDKKTKKGKIFKGSYGNARPKKEKKIQRIKDKIEVPSSTPWPLPFKLI 87
          + CGRGDKKTK+GK FKGSYGNARPK+EKKI+RIKD++EVP STPWPLPFKLI
Sbjct: 30 VTCGRGDKKTKRGKRFKGSYGNARPKREKKIERIKDRVEVPRSTPWPLPFKLI 82

[Close]

BLAST of Carg19383 vs. ExPASy Swiss-Prot
Match: P47910 (30S ribosomal protein S31, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPS31 PE=1 SV=2)

HSP 1 Score: 48.9 bits (115), Expect = 3.3e-05
Identity = 24/44 (54.55%), Postives = 33/44 (75.00%), Query Frame = 0


Query: 21 SLTSSMAVEGLVPIL-CGRGDKKTKKGKIFKGSYGNARPKKEKK 64
          SL+SS++    +P++ CGRGD+KT KGK F  S+GNARPK + K
Sbjct: 38 SLSSSVSTSPALPLIYCGRGDRKTAKGKRFNHSFGNARPKNKNK 81

[Close]

BLAST of Carg19383 vs. ExPASy Swiss-Prot
Match: O80439 (30S ribosomal protein S31, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPS31 PE=1 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 3.7e-04
Identity = 22/45 (48.89%), Postives = 31/45 (68.89%), Query Frame = 0


Query: 34 ILCGRGDKKTKKGKIFKGSYGNARPKKEKKIQRIKDKIEVPSSTP 79
          + CGRGD+KT KGK F  S+GNARP+ + K  R  +++ VP + P
Sbjct: 53 VYCGRGDRKTAKGKRFNHSFGNARPRNKSK-GRGPERVPVPPAPP 96

[Close]

BLAST of Carg19383 vs. ExPASy TrEMBL
Match: A0A6J1GKP4 (30S ribosomal protein S31, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=LOC111455241 PE=3 SV=1)

HSP 1 Score: 170.6 bits (431), Expect = 2.8e-39
Identity = 85/86 (98.84%), Postives = 85/86 (98.84%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGALAKRVMAAER SPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 1  MAQWCGALAKRVMAAERRSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. ExPASy TrEMBL
Match: A0A6J1HXU3 (30S ribosomal protein S31, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC111468449 PE=3 SV=1)

HSP 1 Score: 167.9 bits (424), Expect = 1.8e-38
Identity = 84/86 (97.67%), Postives = 84/86 (97.67%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGALAKRVMAAER SPS TSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 1  MAQWCGALAKRVMAAERRSPSPTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. ExPASy TrEMBL
Match: A0A6J1F3E9 (30S ribosomal protein S31, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111439636 PE=3 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 3.4e-37
Identity = 81/86 (94.19%), Postives = 83/86 (96.51%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          M QWCGA+A+ VMAAER SPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK
Sbjct: 3  MMQWCGAVARGVMAAERRSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 62

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDKIEVPSSTPWPLPFKLI
Sbjct: 63 EKKIQRIKDKIEVPSSTPWPLPFKLI 88

[Close]

BLAST of Carg19383 vs. ExPASy TrEMBL
Match: A0A6J1I644 (30S ribosomal protein S31, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111470248 PE=3 SV=1)

HSP 1 Score: 159.8 bits (403), Expect = 4.9e-36
Identity = 79/86 (91.86%), Postives = 82/86 (95.35%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          M QWCGA+A+ VMAAER SPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGN+RPKK
Sbjct: 1  MMQWCGAVARGVMAAERRSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNSRPKK 60

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKIQRIKDK EVPSSTPWPLPFKLI
Sbjct: 61 EKKIQRIKDKNEVPSSTPWPLPFKLI 86

[Close]

BLAST of Carg19383 vs. ExPASy TrEMBL
Match: A0A5A7SX60 (30S ribosomal protein S31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold1428G001030 PE=3 SV=1)

HSP 1 Score: 157.1 bits (396), Expect = 3.2e-35
Identity = 77/86 (89.53%), Postives = 82/86 (95.35%), Query Frame = 0


Query: 1  MAQWCGALAKRVMAAERGSPSLTSSMAVEGLVPILCGRGDKKTKKGKIFKGSYGNARPKK 60
          MAQWCGA+A+ V+AAER SPSLTSSMAVEGLVPILCGRGDKKTK+GK FKGSYGNARPKK
Sbjct: 3  MAQWCGAVARGVIAAERRSPSLTSSMAVEGLVPILCGRGDKKTKRGKRFKGSYGNARPKK 62

Query: 61 EKKIQRIKDKIEVPSSTPWPLPFKLI 87
          EKKI+RIKDKIEVP STPWPLPFKLI
Sbjct: 63 EKKIERIKDKIEVPRSTPWPLPFKLI 88

[Close]

BLAST of Carg19383 vs. TAIR 10
Match: AT2G21290.1 (unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biological_process unknown; LOCATED IN: mitochondrion; EXPRESSED IN: 22 plant structures; EXPRESSED DURING: 13 growth stages; Has 63 Blast hits to 63 proteins in 12 species: Archae - 0; Bacteria - 0; Metazoa - 0; Fungi - 0; Plants - 63; Viruses - 0; Other Eukaryotes - 0 (source: NCBI BLink). )

HSP 1 Score: 112.5 bits (280), Expect = 1.7e-25
Identity = 56/94 (59.57%), Postives = 66/94 (70.21%), Query Frame = 0


Query: 3  QWCGALAKRVMAAERGSPSLTSSMAVEGLVPI----------LCGRGDKKTKKGKIFKGS 62
          QWCGA+ +R+M  +R S +L  S     L P           LCGRGDKKTKKGK FKGS
Sbjct: 5  QWCGAMTRRIMMTQRTSAALNCSARYSSLSPACAAPAVSEMDLCGRGDKKTKKGKRFKGS 64

Query: 63 YGNARPKKEKKIQRIKDKIEVPSSTPWPLPFKLI 87
          YGN+R KK+K I+RIKDK+E+P STPWPLPFKLI
Sbjct: 65 YGNSRGKKQKMIERIKDKLELPRSTPWPLPFKLI 98

[Close]

BLAST of Carg19383 vs. TAIR 10
Match: AT2G38140.1 (plastid-specific ribosomal protein 4 )

HSP 1 Score: 45.4 bits (106), Expect = 2.6e-05
Identity = 22/45 (48.89%), Postives = 31/45 (68.89%), Query Frame = 0


Query: 34 ILCGRGDKKTKKGKIFKGSYGNARPKKEKKIQRIKDKIEVPSSTP 79
          + CGRGD+KT KGK F  S+GNARP+ + K  R  +++ VP + P
Sbjct: 53 VYCGRGDRKTAKGKRFNHSFGNARPRNKSK-GRGPERVPVPPAPP 96

The following BLAST results are available for this feature:

BLAST of Carg19383 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6572068.1	6.8e-40	100.00	30S ribosomal protein S31, mitochondrial, partial [Cucurbita argyrosperma subsp....	[more]
XP_023554667.1	3.4e-39	98.84	30S ribosomal protein S31, mitochondrial-like [Cucurbita pepo subsp. pepo]	[more]
XP_022952601.1	5.8e-39	98.84	30S ribosomal protein S31, mitochondrial-like [Cucurbita moschata]	[more]
XP_022969451.1	3.7e-38	97.67	30S ribosomal protein S31, mitochondrial-like [Cucurbita maxima]	[more]
XP_022933003.1	7.1e-37	94.19	30S ribosomal protein S31, mitochondrial [Cucurbita moschata] >XP_023529502.1 30...	[more]

BLAST of Carg19383 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SJU8	2.4e-24	59.57	30S ribosomal protein S31, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=At2g...	[more]
P47909	2.8e-20	83.02	30S ribosomal protein S31, mitochondrial OS=Oryza sativa subsp. japonica OX=3994...	[more]
P47910	3.3e-05	54.55	30S ribosomal protein S31, chloroplastic OS=Spinacia oleracea OX=3562 GN=RPS31 P...	[more]
O80439	3.7e-04	48.89	30S ribosomal protein S31, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=RPS3...	[more]

BLAST of Carg19383 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GKP4	2.8e-39	98.84	30S ribosomal protein S31, mitochondrial-like OS=Cucurbita moschata OX=3662 GN=L...	[more]
A0A6J1HXU3	1.8e-38	97.67	30S ribosomal protein S31, mitochondrial-like OS=Cucurbita maxima OX=3661 GN=LOC...	[more]
A0A6J1F3E9	3.4e-37	94.19	30S ribosomal protein S31, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1I644	4.9e-36	91.86	30S ribosomal protein S31, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC11147...	[more]
A0A5A7SX60	3.2e-35	89.53	30S ribosomal protein S31 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffo...	[more]

BLAST of Carg19383 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21290.1	1.7e-25	59.57	unknown protein; FUNCTIONS IN: molecular_function unknown; INVOLVED IN: biologic...	[more]
AT2G38140.1	2.6e-05	48.89	plastid-specific ribosomal protein 4	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR030826	30S ribosomal protein	PFAM	PF17067	RPS31	coord: 30..78 e-value: 1.7E-10 score: 40.9
IPR030826	30S ribosomal protein	TIGRFAM	TIGR04560	TIGR04560	coord: 37..60 e-value: 1.7E-12 score: 45.1
IPR044695	30S ribosomal protein S31, plant	PANTHER	PTHR34550	30S RIBOSOMAL PROTEIN S31, CHLOROPLASTIC	coord: 1..86
None	No IPR available	PANTHER	PTHR34550:SF3	30S RIBOSOMAL PROTEIN S31, MITOCHONDRIAL	coord: 1..86

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19383-RA	Carg19383-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0032544	plastid translation
cellular_component	GO:0009536	plastid
cellular_component	GO:0005840	ribosome