Carg19350 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19350
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	heavy metal-associated isoprenylated plant protein 26-like
Location	Carg_Chr19: 7165394 .. 7166758 (-)
RNA-Seq Expression	Carg19350
Synteny	Carg19350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

TTTAAAACCTCGAAGCTCTTACTTGTTTTGCAAACCAACTGAGAATAAGAAGAAATGGGTGTTTTGGATCATTTGTCTGACTATTTTGATTGTTCTTCCCACAGCTCTAAGCACAAGAAACGCAAGCAATTGCAGGTAAATTTTGAGGTTTTTTGTGTTCTAGATCTTTATTTTGGTTTGATTTTGTGTTTTTTTCTGTTTTTATGCTAAACCCAGATGCTTGTTAGCTTGAAAATGGGGTTTTTTTTGCTTATTTTCTAGCTGTTTTTAGTGAAAGTTTTGAACTTGAACTAAACCCAGTTGAGTTCTCCAAAAACCCATAAAATTAAGCACAAATTTGCTTGGTGGAGTGAAGAAAAAGTAGGAAGATTTGGGCTTTGAACGTACTAGTAGACGTTAGATCTCACATCGGTTGGAGAAGTAAATGAATTATTCTTTATAAAGTTGTGGAAACTTCAATCTAGTAAAAACCCGTTTTAAAAATATTGAGGGGAAGCCCAAAATGAAGAGGTATGTGCTCGCGGTGAGTTCGAACGGTTACGGTGAAATTCTTTATAAAGGTGTGGAAACTTCTAATTAATACACGTATTTCAAAAATTTTGAGTTAGTGGTGGACATGGACTCTTACTGTAGCTGAATGACTTGAGGAAAAACCCGAACGGAAAAGTCATGTGCTAGTGGTGGATTTAGATTGTTACGGGGTGACATTCTTTACAAGAGGGTGAAAATTCTAATGAGTATACGCGTTTTAAAAATTATAAGAGAAAGCCTCAAAAGGGTAAATCAATTGCTAGCGGTGGACTTAGACTGTTATAGTAGTAGATGCGTATGAAAAACTATGAGAGGATGTCGGAGCGAGAAAGTCATCTTTTAGTAGTGGGCTTGAACTATTATTATGTTCACTCTCTCGAGCTGAAATACATGTCAATTACGGTCAAATTAGCGGGAATTCTAATAATATTTGGATGTTGAAATTGTTAGACGGTGGAGCTAAAGATTCGGATCGACTGCGAAGGGTGTGAGAGGAAGGTAAGGAGAGCATTGGAAGGGATGAAGGGAGTGAAGCAGGTGGACGTGGACCGCAAGGCCAACAAAGCCACCGTTGTCGGATACGTCGAGCCATCGAAAGTGGTGGCGCGTGTCGCTCATCGGACTGGTAAGAAGGCGGAGCTATGGCCCTACGTGCCCTACGACGTCGTGGCACATCCTTACGCGCCGGGAGTGTATGACAAGAAGGCGCCGGCAGGGTACGTGCGGAAAGCTGACGATCCCAACGTGTACCAGCTGGCACGTGCGAGCTCCACAGAGGTTCGCTATACGACAGCGTTTAGTGACGAAAATCCCGCGGCTTGCGCTGTCATGTAA

mRNA sequence

TTTAAAACCTCGAAGCTCTTACTTGTTTTGCAAACCAACTGAGAATAAGAAGAAATGGGTGTTTTGGATCATTTGTCTGACTATTTTGATTGTTCTTCCCACAGCTCTAAGCACAAGAAACGCAAGCAATTGCAGACGGTGGAGCTAAAGATTCGGATCGACTGCGAAGGGTGTGAGAGGAAGGTAAGGAGAGCATTGGAAGGGATGAAGGGAGTGAAGCAGGTGGACGTGGACCGCAAGGCCAACAAAGCCACCGTTGTCGGATACGTCGAGCCATCGAAAGTGGTGGCGCGTGTCGCTCATCGGACTGGTAAGAAGGCGGAGCTATGGCCCTACGTGCCCTACGACGTCGTGGCACATCCTTACGCGCCGGGAGTGTATGACAAGAAGGCGCCGGCAGGGTACGTGCGGAAAGCTGACGATCCCAACGTGTACCAGCTGGCACGTGCGAGCTCCACAGAGGTTCGCTATACGACAGCGTTTAGTGACGAAAATCCCGCGGCTTGCGCTGTCATGTAA

Coding sequence (CDS)

ATGGGTGTTTTGGATCATTTGTCTGACTATTTTGATTGTTCTTCCCACAGCTCTAAGCACAAGAAACGCAAGCAATTGCAGACGGTGGAGCTAAAGATTCGGATCGACTGCGAAGGGTGTGAGAGGAAGGTAAGGAGAGCATTGGAAGGGATGAAGGGAGTGAAGCAGGTGGACGTGGACCGCAAGGCCAACAAAGCCACCGTTGTCGGATACGTCGAGCCATCGAAAGTGGTGGCGCGTGTCGCTCATCGGACTGGTAAGAAGGCGGAGCTATGGCCCTACGTGCCCTACGACGTCGTGGCACATCCTTACGCGCCGGGAGTGTATGACAAGAAGGCGCCGGCAGGGTACGTGCGGAAAGCTGACGATCCCAACGTGTACCAGCTGGCACGTGCGAGCTCCACAGAGGTTCGCTATACGACAGCGTTTAGTGACGAAAATCCCGCGGCTTGCGCTGTCATGTAA

Protein sequence

MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVDRKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRKADDPNVYQLARASSTEVRYTTAFSDENPAACAVM

Homology

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BLAST of Carg19350 vs. NCBI nr
Match: XP_022952479.1 (heavy metal-associated isoprenylated plant protein 26-like [Cucurbita moschata] >XP_022969515.1 heavy metal-associated isoprenylated plant protein 26-like [Cucurbita maxima] >XP_023553686.1 heavy metal-associated isoprenylated plant protein 26-like [Cucurbita pepo subsp. pepo] >KAG6572113.1 Heavy metal-associated isoprenylated plant protein 26, partial [Cucurbita argyrosperma subsp. sororia] >KAG7011766.1 Heavy metal-associated isoprenylated plant protein 26 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 312.8 bits (800), Expect = 1.7e-81
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 154

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BLAST of Carg19350 vs. NCBI nr
Match: XP_004144847.1 (heavy metal-associated isoprenylated plant protein 26 [Cucumis sativus] >KGN43252.1 hypothetical protein Csa_020449 [Cucumis sativus])

HSP 1 Score: 308.1 bits (788), Expect = 4.2e-80
Identity = 151/154 (98.05%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSH  KHKKRKQLQTVELKIRIDCEGCERKV+RALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHGHKHKKRKQLQTVELKIRIDCEGCERKVKRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 154

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BLAST of Carg19350 vs. NCBI nr
Match: XP_008447957.1 (PREDICTED: heavy metal-associated isoprenylated plant protein 26-like [Cucumis melo] >KAA0049755.1 heavy metal-associated isoprenylated plant protein 26-like [Cucumis melo var. makuwa] >TYK12123.1 heavy metal-associated isoprenylated plant protein 26-like [Cucumis melo var. makuwa])

HSP 1 Score: 307.4 bits (786), Expect = 7.1e-80
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSH SKHKKRKQLQTVELKIRIDCEGCERKV+RALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHGSKHKKRKQLQTVELKIRIDCEGCERKVKRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RK+NKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKSNKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAAC VM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACVVM 154

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BLAST of Carg19350 vs. NCBI nr
Match: XP_038887000.1 (heavy metal-associated isoprenylated plant protein 26-like [Benincasa hispida])

HSP 1 Score: 307.0 bits (785), Expect = 9.2e-80
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLS+YFDCSSH SKHKKRKQLQTVELKIRIDCEGCERKV+RALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSEYFDCSSHGSKHKKRKQLQTVELKIRIDCEGCERKVKRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARV HRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVTHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 154

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BLAST of Carg19350 vs. NCBI nr
Match: XP_022928351.1 (heavy metal-associated isoprenylated plant protein 26-like [Cucurbita moschata] >XP_023529807.1 heavy metal-associated isoprenylated plant protein 26-like [Cucurbita pepo subsp. pepo] >KAG6589012.1 Heavy metal-associated isoprenylated plant protein 26, partial [Cucurbita argyrosperma subsp. sororia] >KAG7015275.1 Heavy metal-associated isoprenylated plant protein 26 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 303.9 bits (777), Expect = 7.8e-79
Identity = 148/154 (96.10%), Postives = 150/154 (97.40%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDH S+YFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVK VD+D
Sbjct: 1   MGVLDHFSEYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKTVDID 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARV HRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVTHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPN YQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNAYQLARASSTEVRYTTAFSDENPAACAVM 154

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BLAST of Carg19350 vs. ExPASy Swiss-Prot
Match: Q9SZN7 (Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana OX=3702 GN=HIPP26 PE=1 SV=1)

HSP 1 Score: 251.5 bits (641), Expect = 6.0e-66
Identity = 121/154 (78.57%), Postives = 138/154 (89.61%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDH+S+ FDC SH  K KKRKQLQTVE+K+++DCEGCERKVRR++EGMKGV  V ++
Sbjct: 1   MGVLDHVSEMFDC-SHGHKIKKRKQLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLE 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
            KA+K TVVGYV+P+KVVAR++HRTGKK ELWPYVPYDVVAHPYA GVYDKKAP+GYVR+
Sbjct: 61  PKAHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRR 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
            DDP V QLARASSTEVRYTTAFSDENPAAC VM
Sbjct: 121 VDDPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

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BLAST of Carg19350 vs. ExPASy Swiss-Prot
Match: O49613 (Heavy metal-associated isoprenylated plant protein 25 OS=Arabidopsis thaliana OX=3702 GN=HIPP25 PE=2 SV=1)

HSP 1 Score: 225.3 bits (573), Expect = 4.6e-58
Identity = 110/155 (70.97%), Postives = 132/155 (85.16%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDH+S+YFDCS  SS  K+ K LQTV++++ IDCEGCERKVRRALEGM+G++ V ++
Sbjct: 1   MGVLDHVSEYFDCSHGSS--KRHKSLQTVDVRVLIDCEGCERKVRRALEGMRGIRDVTIE 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
             A K TVVGYVEP+KVVAR+ HRTGK+AEL+P+VPYDVVAHPYA GVYD +AP GYVR 
Sbjct: 61  PNAQKVTVVGYVEPNKVVARIIHRTGKRAELYPFVPYDVVAHPYASGVYDNRAPTGYVRN 120

Query: 121 AD-DPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
            + DP+V +LARASSTEVRYTTAFSDEN +AC VM
Sbjct: 121 TEYDPHVSRLARASSTEVRYTTAFSDENASACVVM 153

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BLAST of Carg19350 vs. ExPASy Swiss-Prot
Match: Q67ZW1 (Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana OX=3702 GN=HIPP27 PE=1 SV=1)

HSP 1 Score: 197.2 bits (500), Expect = 1.4e-49
Identity = 96/136 (70.59%), Postives = 113/136 (83.09%), Query Frame = 0


Query: 20  HKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVDRKANKATVVGYVEPSKVVA 79
           HKK KQ Q VE+K+++DCEGCER+VR+++EGMKGV +V VD K +K TV G+V+PSKVV 
Sbjct: 12  HKKLKQFQKVEIKVKMDCEGCERRVRKSVEGMKGVSKVTVDPKQSKLTVEGFVQPSKVVH 71

Query: 80  RVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRKA-DDPNVYQLARASSTEVR 139
           RV HRTGKKAELWPYVPY+VV HPYAPG YDKKAP GYVR A  DP V  LARASS EV+
Sbjct: 72  RVMHRTGKKAELWPYVPYEVVPHPYAPGAYDKKAPPGYVRNALADPLVAPLARASSFEVK 131

Query: 140 YTTAFSDENPAACAVM 155
           YT+AFSD+NP AC +M
Sbjct: 132 YTSAFSDDNPNACTIM 147

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BLAST of Carg19350 vs. ExPASy Swiss-Prot
Match: Q9C9A3 (Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX=3702 GN=HIPP20 PE=1 SV=1)

HSP 1 Score: 174.5 bits (441), Expect = 9.4e-43
Identity = 89/155 (57.42%), Postives = 114/155 (73.55%), Query Frame = 0


Query: 1   MGVLDHLSDYF-DCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDV 60
           MG LD LS+Y  D    + K +KRK +QTV +K+++DC+GCER+V+ A+  MKGVK V+V
Sbjct: 1   MGALDSLSEYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEV 60

Query: 61  DRKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVR 120
           +RK +K TV GYVEP KV+ R+  RTGKKAE+WPYVPY++VA+PYA G YDKKAPAGYVR
Sbjct: 61  NRKIHKVTVSGYVEPKKVLKRI-ERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVR 120

Query: 121 KADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           K++   +  L  A   E  Y + FSDENP AC VM
Sbjct: 121 KSEQSQLQLLPGA--PENHYISLFSDENPNACTVM 152

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BLAST of Carg19350 vs. ExPASy Swiss-Prot
Match: Q93VP2 (Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX=3702 GN=HIPP22 PE=1 SV=1)

HSP 1 Score: 163.7 bits (413), Expect = 1.7e-39
Identity = 82/156 (52.56%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSS--KHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVD 60
           MG L++LS+YF    + S  K KKRK +QTV +K++IDC+GCERK++ A+  +KG K V+
Sbjct: 1   MGALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVE 60

Query: 61  VDRKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYV 120
           V+RK +K TV GYV+P KV+  V     KKAELWPYVPY +VA+PYA G YDK+AP G+V
Sbjct: 61  VNRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFV 120

Query: 121 RKADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           RK++       A+  ST+ +  + FSDENP AC VM
Sbjct: 121 RKSEQAQ----AQPGSTDDKLMSLFSDENPNACTVM 152

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BLAST of Carg19350 vs. ExPASy TrEMBL
Match: A0A6J1I049 (heavy metal-associated isoprenylated plant protein 26-like OS=Cucurbita maxima OX=3661 GN=LOC111468490 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 8.2e-82
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 154

[Close]

BLAST of Carg19350 vs. ExPASy TrEMBL
Match: A0A6J1GLU0 (heavy metal-associated isoprenylated plant protein 26-like OS=Cucurbita moschata OX=3662 GN=LOC111455154 PE=4 SV=1)

HSP 1 Score: 312.8 bits (800), Expect = 8.2e-82
Identity = 154/154 (100.00%), Postives = 154/154 (100.00%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 154

[Close]

BLAST of Carg19350 vs. ExPASy TrEMBL
Match: A0A0A0K4P1 (HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G012410 PE=4 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 2.0e-80
Identity = 151/154 (98.05%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSH  KHKKRKQLQTVELKIRIDCEGCERKV+RALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHGHKHKKRKQLQTVELKIRIDCEGCERKVKRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 154

[Close]

BLAST of Carg19350 vs. ExPASy TrEMBL
Match: A0A5A7U817 (Heavy metal-associated isoprenylated plant protein 26-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold106G00580 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.4e-80
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSH SKHKKRKQLQTVELKIRIDCEGCERKV+RALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHGSKHKKRKQLQTVELKIRIDCEGCERKVKRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RK+NKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKSNKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAAC VM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACVVM 154

[Close]

BLAST of Carg19350 vs. ExPASy TrEMBL
Match: A0A1S3BIL5 (heavy metal-associated isoprenylated plant protein 26-like OS=Cucumis melo OX=3656 GN=LOC103490286 PE=4 SV=1)

HSP 1 Score: 307.4 bits (786), Expect = 3.4e-80
Identity = 150/154 (97.40%), Postives = 152/154 (98.70%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDHLSDYFDCSSH SKHKKRKQLQTVELKIRIDCEGCERKV+RALEGMKGVKQVDVD
Sbjct: 1   MGVLDHLSDYFDCSSHGSKHKKRKQLQTVELKIRIDCEGCERKVKRALEGMKGVKQVDVD 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
           RK+NKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK
Sbjct: 61  RKSNKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           ADDPNVYQLARASSTEVRYTTAFSDENPAAC VM
Sbjct: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACVVM 154

[Close]

BLAST of Carg19350 vs. TAIR 10
Match: AT4G38580.1 (farnesylated protein 6 )

HSP 1 Score: 251.5 bits (641), Expect = 4.3e-67
Identity = 121/154 (78.57%), Postives = 138/154 (89.61%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDH+S+ FDC SH  K KKRKQLQTVE+K+++DCEGCERKVRR++EGMKGV  V ++
Sbjct: 1   MGVLDHVSEMFDC-SHGHKIKKRKQLQTVEIKVKMDCEGCERKVRRSVEGMKGVSSVTLE 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
            KA+K TVVGYV+P+KVVAR++HRTGKK ELWPYVPYDVVAHPYA GVYDKKAP+GYVR+
Sbjct: 61  PKAHKVTVVGYVDPNKVVARMSHRTGKKVELWPYVPYDVVAHPYAAGVYDKKAPSGYVRR 120

Query: 121 ADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
            DDP V QLARASSTEVRYTTAFSDENPAAC VM
Sbjct: 121 VDDPGVSQLARASSTEVRYTTAFSDENPAACVVM 153

[Close]

BLAST of Carg19350 vs. TAIR 10
Match: AT4G35060.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 225.3 bits (573), Expect = 3.3e-59
Identity = 110/155 (70.97%), Postives = 132/155 (85.16%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVD 60
           MGVLDH+S+YFDCS  SS  K+ K LQTV++++ IDCEGCERKVRRALEGM+G++ V ++
Sbjct: 1   MGVLDHVSEYFDCSHGSS--KRHKSLQTVDVRVLIDCEGCERKVRRALEGMRGIRDVTIE 60

Query: 61  RKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRK 120
             A K TVVGYVEP+KVVAR+ HRTGK+AEL+P+VPYDVVAHPYA GVYD +AP GYVR 
Sbjct: 61  PNAQKVTVVGYVEPNKVVARIIHRTGKRAELYPFVPYDVVAHPYASGVYDNRAPTGYVRN 120

Query: 121 AD-DPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
            + DP+V +LARASSTEVRYTTAFSDEN +AC VM
Sbjct: 121 TEYDPHVSRLARASSTEVRYTTAFSDENASACVVM 153

[Close]

BLAST of Carg19350 vs. TAIR 10
Match: AT5G66110.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 197.2 bits (500), Expect = 9.6e-51
Identity = 96/136 (70.59%), Postives = 113/136 (83.09%), Query Frame = 0


Query: 20  HKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDVDRKANKATVVGYVEPSKVVA 79
           HKK KQ Q VE+K+++DCEGCER+VR+++EGMKGV +V VD K +K TV G+V+PSKVV 
Sbjct: 12  HKKLKQFQKVEIKVKMDCEGCERRVRKSVEGMKGVSKVTVDPKQSKLTVEGFVQPSKVVH 71

Query: 80  RVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVRKA-DDPNVYQLARASSTEVR 139
           RV HRTGKKAELWPYVPY+VV HPYAPG YDKKAP GYVR A  DP V  LARASS EV+
Sbjct: 72  RVMHRTGKKAELWPYVPYEVVPHPYAPGAYDKKAPPGYVRNALADPLVAPLARASSFEVK 131

Query: 140 YTTAFSDENPAACAVM 155
           YT+AFSD+NP AC +M
Sbjct: 132 YTSAFSDDNPNACTIM 147

[Close]

BLAST of Carg19350 vs. TAIR 10
Match: AT1G71050.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 174.5 bits (441), Expect = 6.7e-44
Identity = 89/155 (57.42%), Postives = 114/155 (73.55%), Query Frame = 0


Query: 1   MGVLDHLSDYF-DCSSHSSKHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVDV 60
           MG LD LS+Y  D    + K +KRK +QTV +K+++DC+GCER+V+ A+  MKGVK V+V
Sbjct: 1   MGALDSLSEYISDYFRVTRKRRKRKVMQTVNIKVKMDCDGCERRVKNAVSSMKGVKSVEV 60

Query: 61  DRKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYVR 120
           +RK +K TV GYVEP KV+ R+  RTGKKAE+WPYVPY++VA+PYA G YDKKAPAGYVR
Sbjct: 61  NRKIHKVTVSGYVEPKKVLKRI-ERTGKKAEIWPYVPYNMVAYPYAVGTYDKKAPAGYVR 120

Query: 121 KADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           K++   +  L  A   E  Y + FSDENP AC VM
Sbjct: 121 KSEQSQLQLLPGA--PENHYISLFSDENPNACTVM 152

[Close]

BLAST of Carg19350 vs. TAIR 10
Match: AT1G22990.1 (Heavy metal transport/detoxification superfamily protein )

HSP 1 Score: 163.7 bits (413), Expect = 1.2e-40
Identity = 82/156 (52.56%), Postives = 110/156 (70.51%), Query Frame = 0


Query: 1   MGVLDHLSDYFDCSSHSS--KHKKRKQLQTVELKIRIDCEGCERKVRRALEGMKGVKQVD 60
           MG L++LS+YF    + S  K KKRK +QTV +K++IDC+GCERK++ A+  +KG K V+
Sbjct: 1   MGALNYLSEYFSNHFYVSIRKRKKRKVMQTVNIKVKIDCDGCERKIKNAVSSIKGAKSVE 60

Query: 61  VDRKANKATVVGYVEPSKVVARVAHRTGKKAELWPYVPYDVVAHPYAPGVYDKKAPAGYV 120
           V+RK +K TV GYV+P KV+  V     KKAELWPYVPY +VA+PYA G YDK+AP G+V
Sbjct: 61  VNRKMHKVTVSGYVDPKKVLKTVQSTGKKKAELWPYVPYTMVAYPYAAGAYDKRAPPGFV 120

Query: 121 RKADDPNVYQLARASSTEVRYTTAFSDENPAACAVM 155
           RK++       A+  ST+ +  + FSDENP AC VM
Sbjct: 121 RKSEQAQ----AQPGSTDDKLMSLFSDENPNACTVM 152

The following BLAST results are available for this feature:

BLAST of Carg19350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022952479.1	1.7e-81	100.00	heavy metal-associated isoprenylated plant protein 26-like [Cucurbita moschata] ...	[more]
XP_004144847.1	4.2e-80	98.05	heavy metal-associated isoprenylated plant protein 26 [Cucumis sativus] >KGN4325...	[more]
XP_008447957.1	7.1e-80	97.40	PREDICTED: heavy metal-associated isoprenylated plant protein 26-like [Cucumis m...	[more]
XP_038887000.1	9.2e-80	97.40	heavy metal-associated isoprenylated plant protein 26-like [Benincasa hispida]	[more]
XP_022928351.1	7.8e-79	96.10	heavy metal-associated isoprenylated plant protein 26-like [Cucurbita moschata] ...	[more]

BLAST of Carg19350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SZN7	6.0e-66	78.57	Heavy metal-associated isoprenylated plant protein 26 OS=Arabidopsis thaliana OX...	[more]
O49613	4.6e-58	70.97	Heavy metal-associated isoprenylated plant protein 25 OS=Arabidopsis thaliana OX...	[more]
Q67ZW1	1.4e-49	70.59	Heavy metal-associated isoprenylated plant protein 27 OS=Arabidopsis thaliana OX...	[more]
Q9C9A3	9.4e-43	57.42	Heavy metal-associated isoprenylated plant protein 20 OS=Arabidopsis thaliana OX...	[more]
Q93VP2	1.7e-39	52.56	Heavy metal-associated isoprenylated plant protein 22 OS=Arabidopsis thaliana OX...	[more]

BLAST of Carg19350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1I049	8.2e-82	100.00	heavy metal-associated isoprenylated plant protein 26-like OS=Cucurbita maxima O...	[more]
A0A6J1GLU0	8.2e-82	100.00	heavy metal-associated isoprenylated plant protein 26-like OS=Cucurbita moschata...	[more]
A0A0A0K4P1	2.0e-80	98.05	HMA domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_7G012410 PE=4 SV...	[more]
A0A5A7U817	3.4e-80	97.40	Heavy metal-associated isoprenylated plant protein 26-like OS=Cucumis melo var. ...	[more]
A0A1S3BIL5	3.4e-80	97.40	heavy metal-associated isoprenylated plant protein 26-like OS=Cucumis melo OX=36...	[more]

BLAST of Carg19350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G38580.1	4.3e-67	78.57	farnesylated protein 6	[more]
AT4G35060.1	3.3e-59	70.97	Heavy metal transport/detoxification superfamily protein	[more]
AT5G66110.1	9.6e-51	70.59	Heavy metal transport/detoxification superfamily protein	[more]
AT1G71050.1	6.7e-44	57.42	Heavy metal transport/detoxification superfamily protein	[more]
AT1G22990.1	1.2e-40	52.56	Heavy metal transport/detoxification superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.70.100		coord: 23..90 e-value: 1.3E-18 score: 69.2
None	No IPR available	PANTHER	PTHR22814	COPPER TRANSPORT PROTEIN ATOX1-RELATED	coord: 2..154
None	No IPR available	PANTHER	PTHR22814:SF302	HEAVY METAL-ASSOCIATED ISOPRENYLATED PLANT PROTEIN 26	coord: 2..154
IPR006121	Heavy metal-associated domain, HMA	PFAM	PF00403	HMA	coord: 33..87 e-value: 3.4E-13 score: 49.8
IPR006121	Heavy metal-associated domain, HMA	PROSITE	PS50846	HMA_2	coord: 35..91 score: 10.160483
IPR006121	Heavy metal-associated domain, HMA	CDD	cd00371	HMA	coord: 35..90 e-value: 6.26096E-14 score: 60.6973
IPR036163	Heavy metal-associated domain superfamily	SUPERFAMILY	55008	HMA, heavy metal-associated domain	coord: 27..93

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19350-RA	Carg19350-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0046872	metal ion binding