Carg19319 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19319
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	alpha/beta-Hydrolases superfamily protein
Location	Carg_Chr03: 10011188 .. 10012174 (-)
RNA-Seq Expression	Carg19319
Synteny	Carg19319

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCGCACCCAATAAACCAGGCCAACGAGAACAGTCCCTATGGAGGCCTAACGAGGGAAGAGTTCTATAAGAAACACAATGTTACTCACCAGGAAACCTTCATTCTCAATGCCCAGAAGATGAAAATCTTCACTCAGTCATGGCGGCCTGTTTCTGACTCAGAGCTCAAAGGGGTTGTCGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATGTTTGAGCTCACTGCCGTTGCCATAGCCAAATCTGGGTTCCTTGTTTGCTCTCTCGACCTTCAAGGCCATGGCCGATCCGACGGCGCCCCTGGTTCGATCCCAGACATCGAACTACTCGTTCTCGACTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTACCCTCCCTGCTTTTCTGTACGGGGAATCGCTAGGCGGTGCGATTTCGATCCTGATTTGCTTGAAGCAAGAGGGGGTTTGGAGCGGAATTGTGTTAAACGGCTCCATGTGTGGAATCTCGGCGAAATTCAGACCGATTTGGCCATTGGAGAAGCTTCTTCCAATTGCAGCGTCTTTAGCGCCGTGGCTGCGGTTGGTGGTATCAAAGCCCCACGCAAGCAAGTCGTACAAGGAGGAGTGGAAGAGGAGATTGGTGGCAAAAAACCCGAACCGACGATTTTCAGGAAAGCCACCAATGGCGACAGCATTGGAGTTTCTCAGAGTGTGCGAGTACATAAAAATGAACTGCCATGAAATCAGGGTTCCAATGTTGATGGTTCACGGGGAAGACGATGTGGTTTGCGACAGCGAGTCGGCGAGATTTGTGTTCGAATCGGCGGCGAGTGAGGACAAGACGTTGAAGGTTTATCCAGGAATGTGGCACCAGTTGATAGGCGAGGCGAATGAGAATGTGGAGATTGTTTATGCCACCATTTTCAACTGGTTAGTGGATAGAGCAGAGAAGGCCAAGGCCAAGAACAGGAGCAAGAACACCAAAAAGTGA

mRNA sequence

ATGGCGCACCCAATAAACCAGGCCAACGAGAACAGTCCCTATGGAGGCCTAACGAGGGAAGAGTTCTATAAGAAACACAATGTTACTCACCAGGAAACCTTCATTCTCAATGCCCAGAAGATGAAAATCTTCACTCAGTCATGGCGGCCTGTTTCTGACTCAGAGCTCAAAGGGGTTGTCGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATGTTTGAGCTCACTGCCGTTGCCATAGCCAAATCTGGGTTCCTTGTTTGCTCTCTCGACCTTCAAGGCCATGGCCGATCCGACGGCGCCCCTGGTTCGATCCCAGACATCGAACTACTCGTTCTCGACTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTACCCTCCCTGCTTTTCTGTACGGGGAATCGCTAGGCGGTGCGATTTCGATCCTGATTTGCTTGAAGCAAGAGGGGGTTTGGAGCGGAATTGTGTTAAACGGCTCCATGTGTGGAATCTCGGCGAAATTCAGACCGATTTGGCCATTGGAGAAGCTTCTTCCAATTGCAGCGTCTTTAGCGCCGTGGCTGCGGTTGGTGGTATCAAAGCCCCACGCAAGCAAGTCGTACAAGGAGGAGTGGAAGAGGAGATTGGTGGCAAAAAACCCGAACCGACGATTTTCAGGAAAGCCACCAATGGCGACAGCATTGGAGTTTCTCAGAGTGTGCGAGTACATAAAAATGAACTGCCATGAAATCAGGGTTCCAATGTTGATGGTTCACGGGGAAGACGATGTGGTTTGCGACAGCGAGTCGGCGAGATTTGTGTTCGAATCGGCGGCGAGTGAGGACAAGACGTTGAAGGTTTATCCAGGAATGTGGCACCAGTTGATAGGCGAGGCGAATGAGAATGTGGAGATTGTTTATGCCACCATTTTCAACTGGTTAGTGGATAGAGCAGAGAAGGCCAAGGCCAAGAACAGGAGCAAGAACACCAAAAAGTGA

Coding sequence (CDS)

ATGGCGCACCCAATAAACCAGGCCAACGAGAACAGTCCCTATGGAGGCCTAACGAGGGAAGAGTTCTATAAGAAACACAATGTTACTCACCAGGAAACCTTCATTCTCAATGCCCAGAAGATGAAAATCTTCACTCAGTCATGGCGGCCTGTTTCTGACTCAGAGCTCAAAGGGGTTGTCGCCATGGTCCATGGCTACACCTCTGACAGTGGCTGGATGTTTGAGCTCACTGCCGTTGCCATAGCCAAATCTGGGTTCCTTGTTTGCTCTCTCGACCTTCAAGGCCATGGCCGATCCGACGGCGCCCCTGGTTCGATCCCAGACATCGAACTACTCGTTCTCGACTGTACTCTGTTTTTCGACTCTGTTCGAGAACAGAATCCTACCCTCCCTGCTTTTCTGTACGGGGAATCGCTAGGCGGTGCGATTTCGATCCTGATTTGCTTGAAGCAAGAGGGGGTTTGGAGCGGAATTGTGTTAAACGGCTCCATGTGTGGAATCTCGGCGAAATTCAGACCGATTTGGCCATTGGAGAAGCTTCTTCCAATTGCAGCGTCTTTAGCGCCGTGGCTGCGGTTGGTGGTATCAAAGCCCCACGCAAGCAAGTCGTACAAGGAGGAGTGGAAGAGGAGATTGGTGGCAAAAAACCCGAACCGACGATTTTCAGGAAAGCCACCAATGGCGACAGCATTGGAGTTTCTCAGAGTGTGCGAGTACATAAAAATGAACTGCCATGAAATCAGGGTTCCAATGTTGATGGTTCACGGGGAAGACGATGTGGTTTGCGACAGCGAGTCGGCGAGATTTGTGTTCGAATCGGCGGCGAGTGAGGACAAGACGTTGAAGGTTTATCCAGGAATGTGGCACCAGTTGATAGGCGAGGCGAATGAGAATGTGGAGATTGTTTATGCCACCATTTTCAACTGGTTAGTGGATAGAGCAGAGAAGGCCAAGGCCAAGAACAGGAGCAAGAACACCAAAAAGTGA

Protein sequence

MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVEIVYATIFNWLVDRAEKAKAKNRSKNTKK

Homology

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BLAST of Carg19319 vs. NCBI nr
Match: KAG6581477.1 (Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG7034768.1 Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672.5 bits (1734), Expect = 1.8e-189
Identity = 328/328 (100.00%), Postives = 328/328 (100.00%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           IVYATIFNWLVDRAEKAKAKNRSKNTKK
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 328

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BLAST of Carg19319 vs. NCBI nr
Match: XP_022925667.1 (caffeoylshikimate esterase-like [Cucurbita moschata])

HSP 1 Score: 667.9 bits (1722), Expect = 4.4e-188
Identity = 325/328 (99.09%), Postives = 326/328 (99.39%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTREEFYKKHNVTH ETFILNAQKMKIFTQSWRP SDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           IVYATIFNWLVDRAEKAKAKN+SKNTKK
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKNTKK 328

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BLAST of Carg19319 vs. NCBI nr
Match: XP_023543932.1 (caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 649.8 bits (1675), Expect = 1.2e-182
Identity = 315/328 (96.04%), Postives = 324/328 (98.78%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE+FYKKHNVTH E FILNAQ+MKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREQFYKKHNVTHHEAFILNAQEMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAK+GFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKAGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVLSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAAS+DKTLKVYPGMWHQLIGEANE+VE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASKDKTLKVYPGMWHQLIGEANESVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           IVY TIFNWLVDRAEKAKAKN++KNTKK
Sbjct: 301 IVYTTIFNWLVDRAEKAKAKNKNKNTKK 328

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BLAST of Carg19319 vs. NCBI nr
Match: XP_022977253.1 (caffeoylshikimate esterase-like [Cucurbita maxima])

HSP 1 Score: 646.7 bits (1667), Expect = 1.0e-181
Identity = 316/328 (96.34%), Postives = 324/328 (98.78%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE+FY+KHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREDFYQKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVA+AKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVALAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVC+YI
Sbjct: 181 LPIAASLAPSLRLVASKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCKYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           IVYATIFNWLVDRAE  KAKN++K+TKK
Sbjct: 301 IVYATIFNWLVDRAE--KAKNKNKDTKK 326

[Close]

BLAST of Carg19319 vs. NCBI nr
Match: XP_038881607.1 (caffeoylshikimate esterase-like [Benincasa hispida])

HSP 1 Score: 596.3 bits (1536), Expect = 1.6e-166
Identity = 292/326 (89.57%), Postives = 304/326 (93.25%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           M HPINQANENSPYGGLTREEFYKKH VTH E+FILNAQKMKIFTQSWRP SDSELKGVV
Sbjct: 1   MLHPINQANENSPYGGLTREEFYKKHKVTHHESFILNAQKMKIFTQSWRPDSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNP LPAFLYGESLGGAISILI LKQ+GVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSVREQNPNLPAFLYGESLGGAISILISLKQKGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV++KP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVITKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SARFVFESA SEDKTLKVYPGMWHQLIGE  E+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARFVFESAESEDKTLKVYPGMWHQLIGEPKEDVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNT 327
           +VY TIF WLVDRAEK    N+  NT
Sbjct: 301 VVYGTIFTWLVDRAEKTN-NNKIINT 325

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BLAST of Carg19319 vs. ExPASy Swiss-Prot
Match: Q9C942 (Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 2.1e-47
Identity = 107/311 (34.41%), Postives = 174/311 (55.95%), Query Frame = 0


Query: 14  YGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWM 73
           +G +  EE+Y    V + +++       K+FTQS+ P+ D E+KG V M HGY SD+ WM
Sbjct: 23  WGTMPEEEYYTSQGVRNSKSY-FETPNGKLFTQSFLPL-DGEIKGTVYMSHGYGSDTSWM 82

Query: 74  FELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNP--TLP 133
           F+   ++ +  G+ V + DL GHGRSDG    + D+E +      FF  VR  +P   LP
Sbjct: 83  FQKICMSFSSWGYAVFAADLLGHGRSDGIRCYMGDMEKVAATSLAFFKHVRCSDPYKDLP 142

Query: 134 AFLYGESLGGAISILICLKQE-GVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPW 193
           AFL+GES+GG +++L+  + E   W+G++ +  +  I    +P         +   LA  
Sbjct: 143 AFLFGESMGGLVTLLMYFQSEPETWTGLMFSAPLFVIPEDMKPSKAHLFAYGLLFGLADT 202

Query: 194 LRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVP 253
              +       K+ K+  K +++A NP +R++GKP + T  E LR  +Y++ N  ++ +P
Sbjct: 203 WAAMPDNKMVGKAIKDPEKLKIIASNP-QRYTGKPRVGTMRELLRKTQYVQENFGKVTIP 262

Query: 254 MLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLI-GEANENVEIVYATIFNW 313
           +   HG  D V    S++ ++E A+S DKTLK+Y GM+H LI GE +EN EIV   +  W
Sbjct: 263 VFTAHGTADGVTCPTSSKLLYEKASSADKTLKIYEGMYHSLIQGEPDENAEIVLKDMREW 322

Query: 314 LVDRAEKAKAK 321
           + ++ +K  +K
Sbjct: 323 IDEKVKKYGSK 330

[Close]

BLAST of Carg19319 vs. ExPASy Swiss-Prot
Match: A0QNZ7 (Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(2)155) OX=246196 GN=MSMEG_0220 PE=1 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 9.3e-24
Identity = 77/290 (26.55%), Postives = 126/290 (43.45%), Query Frame = 0


Query: 29  THQETFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSGFLV 88
           T  E        ++I    W P  D++ +GVV + HGY   +G  +   A     +G LV
Sbjct: 5   TRSEHSFAGVGGVRIVYDVWTP--DTDPRGVVVLAHGYAEHAG-RYHHVAQRFGAAGLLV 64

Query: 89  CSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILIC 148
            +LD +GHGRS G    + D+   V D           +PTLP  + G S+GG I     
Sbjct: 65  YALDHRGHGRSGGKRVHLRDLSEFVEDFRTLVGIAANDHPTLPRIVLGHSMGGGIVFAYG 124

Query: 149 LKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPWLRL-------VVSKPHAS 208
            +  G +S +VL+G          P+  L  +  +   LAP + +       V   P   
Sbjct: 125 ARYPGEYSAMVLSGPAVNAHDGVSPV--LVAVAKVLGKLAPGIPVENLDADAVSRDPEVV 184

Query: 209 KSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVV 268
            +YK +               GK P   A   + + + +      +  P+L+VHG+ D +
Sbjct: 185 AAYKAD----------PMVHHGKLPAGIARALIGLGQSMPQRAAALTAPLLVVHGDKDRL 244

Query: 269 CDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVEIVYATIFNWLV 312
                +R + +  ASED  LKVYPG++H++  E  +  ++V   + +W+V
Sbjct: 245 IPVAGSRLLVDRVASEDVHLKVYPGLYHEVFNEPEQ--KLVLDDVTSWIV 277

[Close]

BLAST of Carg19319 vs. ExPASy Swiss-Prot
Match: Q99685 (Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2)

HSP 1 Score: 107.1 bits (266), Expect = 3.9e-22
Identity = 82/289 (28.37%), Postives = 134/289 (46.37%), Query Frame = 0


Query: 35  ILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQ 94
           ++NA    +F + W+P      K ++ + HG    SG   EL  + +     LV + D  
Sbjct: 22  LVNADGQYLFCRYWKPTGTP--KALIFVSHGAGEHSGRYEELARMLMGLD-LLVFAHDHV 81

Query: 95  GHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISILICLKQEGV 154
           GHG+S+G    + D  + V D     DS+++  P LP FL G S+GGAI+IL   ++ G 
Sbjct: 82  GHGQSEGERMVVSDFHVFVRDVLQHVDSMQKDYPGLPVFLLGHSMGGAIAILTAAERPGH 141

Query: 155 WSGIVLNGSM------CGISAKFRPIWPLEKLLPIAASLAPWLRLVVSKPHASKSYKEEW 214
           ++G+VL   +         + K      L  +LP   SL P    V+S        + + 
Sbjct: 142 FAGMVLISPLVLANPESATTFKVLAAKVLNLVLP-NLSLGPIDSSVLS--------RNKT 201

Query: 215 KRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSESAR 274
           +  +   +P    +G   +   ++ L     ++    ++ VP L++ G  D +CDS+ A 
Sbjct: 202 EVDIYNSDPLICRAGL-KVCFGIQLLNAVSRVERALPKLTVPFLLLQGSADRLCDSKGAY 261

Query: 275 FVFESAASEDKTLKVYPGMWHQLIGEANENVEIVYATIFNWLVDRAEKA 318
            + E A S+DKTLK+Y G +H L  E  E    V+  I  W+  R   A
Sbjct: 262 LLMELAKSQDKTLKIYEGAYHVLHKELPEVTNSVFHEINMWVSQRTATA 297

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BLAST of Carg19319 vs. ExPASy Swiss-Prot
Match: O35678 (Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1)

HSP 1 Score: 105.5 bits (262), Expect = 1.1e-21
Identity = 80/296 (27.03%), Postives = 142/296 (47.97%), Query Frame = 0


Query: 27  NVTHQE-TFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSG 86
           NV +Q+   ++NA    +F + W+P      K ++ + HG     G   EL A  +    
Sbjct: 13  NVPYQDLPHLVNADGQYLFCRYWKP--SGTPKALIFVSHGAGEHCGRYDEL-AHMLKGLD 72

Query: 87  FLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISI 146
            LV + D  GHG+S+G    + D ++ V D     D++++  P +P FL G S+GGAISI
Sbjct: 73  MLVFAHDHVGHGQSEGERMVVSDFQVFVRDVLQHVDTIQKDYPDVPIFLLGHSMGGAISI 132

Query: 147 LICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPWL--RLVVSKPHASKS 206
           L+  ++   +SG+VL   +   + +         L  +AA L  ++   + + +  +S  
Sbjct: 133 LVAAERPTYFSGMVLISPLVLANPE-----SASTLKVLAAKLLNFVLPNMTLGRIDSSVL 192

Query: 207 YKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCD 266
            + + +  L   +P    +G   +   ++ L     ++     + +P L++ G  D +CD
Sbjct: 193 SRNKSEVDLYNSDPLVCRAGL-KVCFGIQLLNAVARVERAMPRLTLPFLLLQGSADRLCD 252

Query: 267 SESARFVFESAASEDKTLKVYPGMWHQLIGEANENVEIVYATIFNWLVDRAEKAKA 320
           S+ A  + ES+ S+DKTLK+Y G +H L  E  E    V   + +W+  R   A A
Sbjct: 253 SKGAYLLMESSRSQDKTLKMYEGAYHVLHRELPEVTNSVLHEVNSWVSHRIAAAGA 299

[Close]

BLAST of Carg19319 vs. ExPASy Swiss-Prot
Match: Q8R431 (Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1)

HSP 1 Score: 105.1 bits (261), Expect = 1.5e-21
Identity = 80/296 (27.03%), Postives = 136/296 (45.95%), Query Frame = 0


Query: 27  NVTHQE-TFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSG 86
           NV +Q+   ++NA    +F + W+P      K ++ + HG     G   EL A  + +  
Sbjct: 13  NVPYQDLPHLVNADGQYLFCRYWKP--SGTPKALIFVSHGAGEHCGRYDEL-AQMLKRLD 72

Query: 87  FLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPTLPAFLYGESLGGAISI 146
            LV + D  GHG+S+G    + D ++ V D     ++V++  P +P FL G S+GGAISI
Sbjct: 73  MLVFAHDHVGHGQSEGERMVVSDFQVFVRDLLQHVNTVQKDYPEVPVFLLGHSMGGAISI 132

Query: 147 LICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPWLRLVVSKPHASKSYK 206
           L   ++   +SG++L   +   + +         L  +AA L  ++   +S      S  
Sbjct: 133 LAAAERPTHFSGMILISPLILANPE-----SASTLKVLAAKLLNFVLPNISLGRIDSSVL 192

Query: 207 EEWKRRLVAKNPNRRF-SGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDS 266
              K  +   N +         +   ++ L     ++     + +P L++ G  D +CDS
Sbjct: 193 SRNKSEVDLYNSDPLICHAGVKVCFGIQLLNAVSRVERAMPRLTLPFLLLQGSADRLCDS 252

Query: 267 ESARFVFESAASEDKTLKVYPGMWHQLIGEANENVEIVYATIFNWLVDRAEKAKAK 321
           + A  + ES+ S+DKTLK+Y G +H L  E  E    V   I  W+  R   A A+
Sbjct: 253 KGAYLLMESSPSQDKTLKMYEGAYHVLHKELPEVTNSVLHEINTWVSHRIAVAGAR 300

[Close]

BLAST of Carg19319 vs. ExPASy TrEMBL
Match: A0A6J1ECA8 (caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE=4 SV=1)

HSP 1 Score: 667.9 bits (1722), Expect = 2.1e-188
Identity = 325/328 (99.09%), Postives = 326/328 (99.39%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTREEFYKKHNVTH ETFILNAQKMKIFTQSWRP SDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHHETFILNAQKMKIFTQSWRPASDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           IVYATIFNWLVDRAEKAKAKN+SKNTKK
Sbjct: 301 IVYATIFNWLVDRAEKAKAKNKSKNTKK 328

[Close]

BLAST of Carg19319 vs. ExPASy TrEMBL
Match: A0A6J1IPF3 (caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.1e-182
Identity = 316/328 (96.34%), Postives = 324/328 (98.78%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           MAHPINQANENSPYGGLTRE+FY+KHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV
Sbjct: 1   MAHPINQANENSPYGGLTREDFYQKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYTSDSGW+FELTAVA+AKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF
Sbjct: 61  AMVHGYTSDSGWIFELTAVALAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL
Sbjct: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV SKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVC+YI
Sbjct: 181 LPIAASLAPSLRLVASKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCKYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE
Sbjct: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           IVYATIFNWLVDRAE  KAKN++K+TKK
Sbjct: 301 IVYATIFNWLVDRAE--KAKNKNKDTKK 326

[Close]

BLAST of Carg19319 vs. ExPASy TrEMBL
Match: A0A5A7UE91 (Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009620 PE=4 SV=1)

HSP 1 Score: 587.0 bits (1512), Expect = 4.8e-164
Identity = 283/323 (87.62%), Postives = 301/323 (93.19%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           M+HPINQANENSPYG LTREEFYKKHNVTH E+FILNAQKMKIFTQSWRP SD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGW+FELT VAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGIS KF+PIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+VFE+A SEDKTLKVYPGMWHQLIGEA E+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRS 324
           IVY TI NWLVD+A+KA   N +
Sbjct: 301 IVYGTILNWLVDKADKANNSNNN 323

[Close]

BLAST of Carg19319 vs. ExPASy TrEMBL
Match: A0A0A0KKV9 (Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500 PE=4 SV=1)

HSP 1 Score: 585.1 bits (1507), Expect = 1.8e-163
Identity = 283/328 (86.28%), Postives = 303/328 (92.38%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           M+HPI+QANENSPYG LTREEFYKKHNVTH E+FILNAQKMKIFTQSW+P SDS+LKGVV
Sbjct: 1   MSHPISQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWQPDSDSKLKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGW+FELT +AIAK GFLVCSLDLQGHGRS+GAPGSI DIELLVLDCT FF
Sbjct: 61  AMVHGYTCDSGWIFELTGIAIAKLGFLVCSLDLQGHGRSEGAPGSIRDIELLVLDCTQFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNGSMCGISAKF+PIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGSMCGISAKFKPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           K NCHEIRVP+LMVHGEDDVVCDS SAR+V+E+A SEDKTLKVYPGMWHQLIGE  ENVE
Sbjct: 241 KRNCHEIRVPLLMVHGEDDVVCDSWSARYVYEAAESEDKTLKVYPGMWHQLIGETKENVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRSKNTKK 329
           +VY TIFNWLVDRAEKA     +KN  K
Sbjct: 301 VVYGTIFNWLVDRAEKADNTTTTKNNIK 328

[Close]

BLAST of Carg19319 vs. ExPASy TrEMBL
Match: A0A1S3B390 (caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=1)

HSP 1 Score: 584.7 bits (1506), Expect = 2.4e-163
Identity = 282/323 (87.31%), Postives = 300/323 (92.88%), Query Frame = 0


Query: 1   MAHPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVV 60
           M+HPINQANENSPYG LTREEFYKKHNVTH E+FILNAQKMKIFTQSWRP SD++LKGVV
Sbjct: 1   MSHPINQANENSPYGELTREEFYKKHNVTHHESFILNAQKMKIFTQSWRPDSDTKLKGVV 60

Query: 61  AMVHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFF 120
           AMVHGYT DSGW+FELT VAIAK GFLVCSLDLQGHGRS+GAPGSIPDIELLV DCTLFF
Sbjct: 61  AMVHGYTCDSGWIFELTGVAIAKLGFLVCSLDLQGHGRSEGAPGSIPDIELLVHDCTLFF 120

Query: 121 DSVREQNPTLPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 180
           DS+REQ+P LPAFLYGESLGGAISILI LKQEGVW+GIVLNG MCGIS KF+PIWPLEKL
Sbjct: 121 DSIREQHPNLPAFLYGESLGGAISILISLKQEGVWNGIVLNGFMCGISGKFKPIWPLEKL 180

Query: 181 LPIAASLAPWLRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYI 240
           LPIAASLAP LRLV+SKP ASKSYKEEWKRRLVA+NPNRRFSGKPPMATALEFLRVCEYI
Sbjct: 181 LPIAASLAPSLRLVISKPVASKSYKEEWKRRLVARNPNRRFSGKPPMATALEFLRVCEYI 240

Query: 241 KMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENVE 300
           K NCHEIRVPMLMVHGEDDVVCDS SAR+VFE+A SEDKTLKVYPGMWHQLIGEA E+VE
Sbjct: 241 KRNCHEIRVPMLMVHGEDDVVCDSWSARYVFEAAESEDKTLKVYPGMWHQLIGEAKEDVE 300

Query: 301 IVYATIFNWLVDRAEKAKAKNRS 324
           IVY TI NWLVD+A+KA   N +
Sbjct: 301 IVYGTILNWLVDKADKANNSNNN 323

[Close]

BLAST of Carg19319 vs. TAIR 10
Match: AT5G14980.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 338.2 bits (866), Expect = 7.4e-93
Identity = 156/316 (49.37%), Postives = 224/316 (70.89%), Query Frame = 0


Query: 3   HPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAM 62
           HP+++AN +SP+G L+  EFY +H+VTH   ++ N   +K+FTQ W P++   L G++A+
Sbjct: 4   HPVSEANASSPFGSLSAAEFYSRHSVTHSSAYVTNPTGLKLFTQWWTPLNRPPL-GLIAV 63

Query: 63  VHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDS 122
           VHG+T +S W  +LT+V  AKSG+L C++D QGHG SDG    IP+I L+V DC  FFD 
Sbjct: 64  VHGFTGESSWFLQLTSVLFAKSGYLTCAIDHQGHGFSDGLTAHIPNINLIVDDCISFFDD 123

Query: 123 VREQNPT--LPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 182
            R+++ +  LPAFLY ESLGGAI++ I L+Q+  W+G++L+G+MC IS KF+P WPL+ L
Sbjct: 124 FRKRHASSFLPAFLYSESLGGAIALYITLRQKHQWNGLILSGAMCSISHKFKPPWPLQHL 183

Query: 183 LPIAASLAPWLRLVVSKPH-ASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEY 242
           L +AA+L P  R+V ++   A  S+KE WKR+L   NPNR   GKP  ATA E +RVCE 
Sbjct: 184 LTLAATLIPTWRVVPTRGSIAGVSFKEPWKRKLAYANPNRTV-GKPRAATAYELVRVCED 243

Query: 243 IKMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENV 302
           ++    E+ VP+++VHG DDVVCD  S   ++   +S DKT+K+YPGMWHQLIGE+ ENV
Sbjct: 244 LQNRFEEVEVPLMIVHGRDDVVCDPASVEELYRRCSSRDKTIKIYPGMWHQLIGESEENV 303

Query: 303 EIVYATIFNWLVDRAE 316
           ++V+  + +W+  R+E
Sbjct: 304 DLVFGDVLDWIKTRSE 317

[Close]

BLAST of Carg19319 vs. TAIR 10
Match: AT5G19290.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 333.6 bits (854), Expect = 1.8e-91
Identity = 161/321 (50.16%), Postives = 221/321 (68.85%), Query Frame = 0


Query: 3   HPINQANENSPYGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAM 62
           HPI+ ANE++P+G LT +EFY KH+V+H   FI N + +K+FTQ W P+  ++  G++A+
Sbjct: 4   HPISDANEHNPFGSLTADEFYAKHSVSHSSAFITNPRGLKLFTQWWSPLPPTKPIGIIAV 63

Query: 63  VHGYTSDSGWMFELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDS 122
           VHG+T +S W  +LT++  AKSGF+ C++D QGHG SDG    IPDI  +V DC  FFD 
Sbjct: 64  VHGFTGESSWFLQLTSILFAKSGFITCAIDHQGHGFSDGLIAHIPDINPVVDDCISFFDD 123

Query: 123 VR-EQNPT-LPAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKL 182
            R  Q P+ LP FLY ESLGGAI++ I L+Q GVW G++LNG+MCGIS KF+P WPLE L
Sbjct: 124 FRSRQTPSDLPCFLYSESLGGAIALYISLRQRGVWDGLILNGAMCGISDKFKPPWPLEHL 183

Query: 183 LPIAASLAPWLRLVVSKPH-ASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEY 242
           L + A+L P  R++ ++      S+KE WKR+L   +P RR   KP  ATA E +RVC+ 
Sbjct: 184 LFVVANLIPTWRVIPTRGSIPDVSFKEPWKRKLAMASP-RRTVAKPRAATAYELIRVCKD 243

Query: 243 IKMNCHEIRVPMLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWHQLIGEANENV 302
           ++    E+ VP+L+VHG  DVVCD      +   A SEDKT+K+YP +WHQ+IGE+ E V
Sbjct: 244 LQGRFEEVEVPLLIVHGGGDVVCDVACVEELHRRAISEDKTIKIYPELWHQMIGESEEKV 303

Query: 303 EIVYATIFNWLVDRAE-KAKA 320
           ++VY  + +WL  RAE KA+A
Sbjct: 304 DLVYGDMLSWLKSRAERKARA 323

[Close]

BLAST of Carg19319 vs. TAIR 10
Match: AT1G11090.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 236.1 bits (601), Expect = 4.0e-62
Identity = 121/313 (38.66%), Postives = 188/313 (60.06%), Query Frame = 0


Query: 14  YGGLTREEFYKKHNVTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWM 73
           +G  + E+++    +   ++F  + + + +FT+SW P S S  +G++ MVHGY +D  W 
Sbjct: 13  WGETSEEDYFNLKGIIGSKSFFTSPRGLNLFTRSWLPSSSSPPRGLIFMVHGYGNDVSWT 72

Query: 74  FELTAVAIAKSGFLVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSVREQNPT---L 133
           F+ T + +A+ GF   +LD++GHGRSDG    +P ++L+V D   FF+S++ QNP    L
Sbjct: 73  FQSTPIFLAQMGFACFALDIEGHGRSDGVRAYVPSVDLVVDDIISFFNSIK-QNPKFQGL 132

Query: 134 PAFLYGESLGGAISILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPW 193
           P FL+GES+GGAI +LI       + G VL   MC IS K RP WP+++ L + +   P 
Sbjct: 133 PRFLFGESMGGAICLLIQFADPLGFDGAVLVAPMCKISDKVRPKWPVDQFLIMISRFLPT 192

Query: 194 LRLVVSKPHASKSYKEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVP 253
             +V ++    KS K E K+ +  +NP  R++ KP + T +E LRV +Y+     ++ +P
Sbjct: 193 WAIVPTEDLLEKSIKVEEKKPIAKRNP-MRYNEKPRLGTVMELLRVTDYLGKKLKDVSIP 252

Query: 254 MLMVHGEDDVVCDSESARFVFESAASEDKTLKVYPGMWH-QLIGEANENVEIVYATIFNW 313
            ++VHG  D V D E +R ++E A S+DKTLK+Y GM H  L GE ++N+EIV   I +W
Sbjct: 253 FIIVHGSADAVTDPEVSRELYEHAKSKDKTLKIYDGMMHSMLFGEPDDNIEIVRKDIVSW 312

Query: 314 LVDRAEKAKAKNR 323
           L DR    K K +
Sbjct: 313 LNDRCGGDKTKTQ 323

[Close]

BLAST of Carg19319 vs. TAIR 10
Match: AT2G39400.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 200.7 bits (509), Expect = 1.9e-51
Identity = 107/290 (36.90%), Postives = 166/290 (57.24%), Query Frame = 0


Query: 28  VTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSGFL 87
           V ++E F+LN++ MK+FT  W+PV   E K ++ + HGY  +S       A  +A +GF 
Sbjct: 2   VMYEEDFVLNSRGMKLFTCVWKPVK-QEPKALLFLCHGYAMESSITMNSAATRLANAGFA 61

Query: 88  VCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSV--REQNPTLPAFLYGESLGGAISI 147
           V  +D +GHG+S+G  G I + + LV D +  + ++  RE+N     FL GES+GGA+ +
Sbjct: 62  VYGMDYEGHGKSEGLNGYISNFDDLVDDVSNHYSTICEREENKGKMRFLLGESMGGAVVL 121

Query: 148 LICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPWLRLVVSKPHASKSYK 207
           L+  K+   W G VL   MC ++ + +P   +  +L   A   P  ++V        + K
Sbjct: 122 LLARKKPDFWDGAVLVAPMCKLADEIKPHPVVISILIKLAKFIPTWKIVPGNDIIDIAIK 181

Query: 208 EEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDSE 267
           E   R  V +N    + G+P + TA + L V   ++ N H++ +P +++HGEDD V D  
Sbjct: 182 EPHIRNQVREN-KYCYKGRPRLNTAYQLLLVSLDLEKNLHQVSIPFIVLHGEDDKVTDKS 241

Query: 268 SARFVFESAASEDKTLKVYPGMWHQLI-GEANENVEIVYATIFNWLVDRA 315
            ++ ++E A+S DKT K+YP MWH L+ GE NEN EIV+  I NWL DRA
Sbjct: 242 ISKMLYEVASSSDKTFKLYPKMWHALLYGETNENSEIVFGDIINWLEDRA 289

[Close]

BLAST of Carg19319 vs. TAIR 10
Match: AT2G39420.1 (alpha/beta-Hydrolases superfamily protein )

HSP 1 Score: 198.7 bits (504), Expect = 7.0e-51
Identity = 104/287 (36.24%), Postives = 167/287 (58.19%), Query Frame = 0


Query: 27  NVTHQETFILNAQKMKIFTQSWRPVSDSELKGVVAMVHGYTSDSGWMFELTAVAIAKSGF 86
           N+ ++E+FI N + MK+FT  W P +  E K +V + HGY  +       TA  + K+GF
Sbjct: 7   NIKYEESFIKNTRGMKLFTCKWVP-AKQEPKALVFICHGYAMECSITMNSTARRLVKAGF 66

Query: 87  LVCSLDLQGHGRSDGAPGSIPDIELLVLDCTLFFDSV--REQNPTLPAFLYGESLGGAIS 146
            V  +D +GHG+SDG    +P+ + LV D +  + S+  +E+N     FL GES+GGA+ 
Sbjct: 67  AVYGIDYEGHGKSDGLSAYVPNFDHLVDDVSTHYTSICEKEENKGKMRFLLGESMGGAVL 126

Query: 147 ILICLKQEGVWSGIVLNGSMCGISAKFRPIWPLEKLLPIAASLAPWLRLVVSKPHASKSY 206
           +L+  K+   W G VL   MC I+ + +P   +  +L   + + P  +++  +     ++
Sbjct: 127 LLLHRKKPQFWDGAVLVAPMCKIAEEMKPSPLVISILAKLSGVIPSWKIIPGQDIIETAF 186

Query: 207 KEEWKRRLVAKNPNRRFSGKPPMATALEFLRVCEYIKMNCHEIRVPMLMVHGEDDVVCDS 266
           K+   R+ V +NP   + G+P + TA E LRV   ++   +E+ +P +++HGEDD V D 
Sbjct: 187 KQPEIRKQVRENP-YCYKGRPRLKTAYELLRVSTDLEKRLNEVSLPFIVLHGEDDKVTDK 246

Query: 267 ESARFVFESAASEDKTLKVYPGMWHQLI-GEANENVEIVYATIFNWL 311
             +R ++E A+S DKT K+YPGMWH L+ GE  EN+E V+A I  WL
Sbjct: 247 AVSRQLYEVASSSDKTFKLYPGMWHGLLYGETPENIETVFADIIGWL 291

The following BLAST results are available for this feature:

BLAST of Carg19319 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6581477.1	1.8e-189	100.00	Caffeoylshikimate esterase, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_022925667.1	4.4e-188	99.09	caffeoylshikimate esterase-like [Cucurbita moschata]	[more]
XP_023543932.1	1.2e-182	96.04	caffeoylshikimate esterase-like [Cucurbita pepo subsp. pepo]	[more]
XP_022977253.1	1.0e-181	96.34	caffeoylshikimate esterase-like [Cucurbita maxima]	[more]
XP_038881607.1	1.6e-166	89.57	caffeoylshikimate esterase-like [Benincasa hispida]	[more]

BLAST of Carg19319 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9C942	2.1e-47	34.41	Caffeoylshikimate esterase OS=Arabidopsis thaliana OX=3702 GN=CSE PE=1 SV=1	[more]
A0QNZ7	9.3e-24	26.55	Monoacylglycerol lipase OS=Mycolicibacterium smegmatis (strain ATCC 700084 / mc(...	[more]
Q99685	3.9e-22	28.37	Monoglyceride lipase OS=Homo sapiens OX=9606 GN=MGLL PE=1 SV=2	[more]
O35678	1.1e-21	27.03	Monoglyceride lipase OS=Mus musculus OX=10090 GN=Mgll PE=1 SV=1	[more]
Q8R431	1.5e-21	27.03	Monoglyceride lipase OS=Rattus norvegicus OX=10116 GN=Mgll PE=1 SV=1	[more]

BLAST of Carg19319 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1ECA8	2.1e-188	99.09	caffeoylshikimate esterase-like OS=Cucurbita moschata OX=3662 GN=LOC111433016 PE...	[more]
A0A6J1IPF3	5.1e-182	96.34	caffeoylshikimate esterase-like OS=Cucurbita maxima OX=3661 GN=LOC111477621 PE=4...	[more]
A0A5A7UE91	4.8e-164	87.62	Caffeoylshikimate esterase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A0A0KKV9	1.8e-163	86.28	Hydrolase_4 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_6G525500...	[more]
A0A1S3B390	2.4e-163	87.31	caffeoylshikimate esterase-like OS=Cucumis melo OX=3656 GN=LOC103485302 PE=4 SV=...	[more]

BLAST of Carg19319 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G14980.1	7.4e-93	49.37	alpha/beta-Hydrolases superfamily protein	[more]
AT5G19290.1	1.8e-91	50.16	alpha/beta-Hydrolases superfamily protein	[more]
AT1G11090.1	4.0e-62	38.66	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39400.1	1.9e-51	36.90	alpha/beta-Hydrolases superfamily protein	[more]
AT2G39420.1	7.0e-51	36.24	alpha/beta-Hydrolases superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000073	Alpha/beta hydrolase fold-1	PRINTS	PR00111	ABHYDROLASE	coord: 251..265 score: 30.8 coord: 85..100 score: 37.07 coord: 134..147 score: 40.64
IPR022742	Serine aminopeptidase, S33	PFAM	PF12146	Hydrolase_4	coord: 55..295 e-value: 1.1E-56 score: 191.8
IPR029058	Alpha/Beta hydrolase fold	GENE3D	3.40.50.1820	alpha/beta hydrolase	coord: 19..323 e-value: 9.1E-54 score: 184.7
IPR029058	Alpha/Beta hydrolase fold	SUPERFAMILY	53474	alpha/beta-Hydrolases	coord: 40..313
None	No IPR available	PANTHER	PTHR11614	PHOSPHOLIPASE-RELATED	coord: 2..319
None	No IPR available	PANTHER	PTHR11614:SF101	CAFFEOYLSHIKIMATE ESTERASE-LIKE	coord: 2..319

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19319-RA	Carg19319-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
cellular_component	GO:0016020	membrane
molecular_function	GO:0016298	lipase activity