Carg19290 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview
NameCarg19290
Typegene
OrganismCucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
DescriptionCBL-interacting serine/threonine-protein kinase 23
LocationCarg_Chr03: 9792705 .. 9796094 (+)
RNA-Seq ExpressionCarg19290
SyntenyCarg19290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CAGGTCTGGCAACCGTGTAAGAAGAAGAGGTCTTGATCGGAGGATTTTACAGCGAGGAAAGGGATTGCGGGGTGAAGGAGTTGTTAGAATTGGCGACAGAGATGGCTTTCAGGTCGAGCAGTCATACCAAAACGCGGGTGGGGAGATATGAGCTGGGGAGAACCCTTGGAGAGGGCAGCTTCGCGAAGGTTAAGTTCGCTCGGAATTGCGAGACAGGGGAGAACGTCGCCATTAAAATCCTCAACAAAGAGGACATTCTCAAGCACAAGATGATCGGGCAGGTATAATTTGGATTCGCTACTAATTCAAACATTCATTTATTTTGTTCTACACGCATTAGCCATGATAGTTGGAGGCTAGCAACTAGTCAAACTTTACTGCAAAACTTAATATTCAATTAGGCAATTGAAACACAATCCTATATGCTTTGTTCTTGAGTCTTCAATGTTTTTGCTGAATATTTATCGAAGAAATCTAAGCTTCATTGCATAACTTAGTCTTTTAGTTAAACACTAAGATTATATAATTTAAGCATCAACTCTATTTATGCATATCTGCAGATTAAACGTGAAATATCAACGATGAAACTCATTAGACATCCAAATGTCACTCGAATGTATGAGGTTAGATCAAGTTATGAACTTAATTTTCTTAGTTTGTTCTTGTTTCCTCAATTGTCTTATTGCATCTCATCTGCCACTAATTATTTTCTTGGTGCAGGTGATGGCTAGCAAAAGCAAGATATATATTGTTTTGGAATTTGTAACTGGTGGAGAACTATTCAACAAAATTGTGAGATACAGTGACTGGTTTTATCTCGTATCCATGCATGCAATTGTAACTGGTAGTTGAACATAAATTTATTCCTTTTCCCTTATCCAGGCATGTAATGGAAGATTCAAAGAAGATGAAGCAAGAAAATATTTTCAGCAGCTTATAAATGCTGTGGATTATTGCCATAGCAGAGGCGTTTGTCATCGAGACCTAAAGGTTCATGGCAAATCATCATAGATATCATTCTGTTGTCATTGCTAGTAATATCATCTTGTTAAGCTACAATTTTAAAAGTTCTGGTCTCAAAAATAGTCTTTGTCTATGTTCTTCAGCCAGAGAATTTATTATTGGATGCCAGTGGCGTTCTAAAAGTTTCAGATTTTGGATTGAGTGCTCTACCTCAACAAGTTCGAGTGAGTATGATGCTATGAAAGCTAGCATGCTTTTAGTATGATGGATTTATGAGTAATCCTTATCTCTCTTATTAGGACGATGGATTACTTCACACAACATGTGGAACACCCAATTATGTTGCTCCAGAGGTACAATGGATTTAGACTTCAACTATTTACCACTTGCTCTCCTAAAATTCTTTTGTGATTCAATGCGTTCATAATAGGTTATCAACAATAAAGGCTACTGTGGTGCAAAGGCGGACCTATGGTCATGTGGGGTTATCCTTTTTGTCCTCATGGCAGGCTATTTGCCATTTGAAGAAGCCAGTCTCGTGACATTGTATAAAAAGGTCTTTGTATTTGTAAACATTTAATTTCTCGTTGGTTCTGTGTTCTTATTATTTTTAGTACATCTACAAGCTCAAACCATAGAATTTGGTTTGTGTTATAGATATTCAAGGCCGACTTCAAATGTCCTCCATGGTTCTCCTCTAGTGCCAAGAAATTAATCAAGAGAATCCTCGATCCGAATCCGTTAACTGTACGACTTTGTATATTTAAATACTTCTTCCATCTTAATGATGTTCTTAGTTATTATATGATGTAATCTCTTTGTTGTTAACTTCTTATGAATTTTCTATGAAACAGAGAATTACAATTGCTGAGGTTCTTGAGAATGATTGGTTTAAGAAAGGATACAAGCCACCCACTTTCGAAAACGACGAAATCGTTCTTGATGACGTTGACGACATCTTTAATGAATCAGGGGTAACGCAAGTTGTTCTTTGGCATTGTTTTTTATGAATGTTAAGGGTAGTAGACATGACTTTTCTTATGTTGCAGGTCACTCATAACCTTGTAGTCGAGAAGAGAGAAGAAGGGCTCGCATCCCCTGCATCACCTGTCACTATGAATGCATTCGAGCTCATCTCGAAATCTCAGGGTCTCAACCTTGCCACTCTGTTTGAGAAGCAAATGGTAGGTTATGATCTTTCCACAGCACAAACATCTTATGTTGTGACATTGATGTTTCTCTGCAACCATCCATCTGAAGTTTTTTCCTTGTTTTGTTCATTTATGAATGCAAATTAATATCTGTGCTGATCGGAATTATGGTTACCAGGGGCTATCTAAACGTGAAAAAAGTTTCACATCCCATTGTCCAGCAGATGAAATCCTTTCCAAGATAAAAAAGACAGCAGTTCCTATGGGTTTTGATGTGAAGATCAATAATTTCAAGGTTGGTGGAAGGCTGGAAAATCAAAATCTTTAATAGAATCATCCATAGCTTACCAAACCGATTTTGGACTTAAAATATCCGTTTTGCTTATTACAGATGAAGCTCCAAGGGGAAAAAACTGGACGCAAAGGTCATTTATCAGTTTCAACAGAGGTTCTTCTCTTCACTTTTCCCCTGCATGTCCTTTAAATTCCGGTTGTTTCTTAAAAATTATCATTCTCTAGGCCAACACGAGTATCGTTCAACTGATCTAAAATATAGGACCACCCGAACACCCCACATATTGTTGAACTAAAAAAATTTCTCTCTCCAGATTTTTCAGGTCGCTCCTTCGTTACATATGGTAGAGCTCCGCAAAGCTGGAGGGGATACTTTAGAATTTCACAAGGTATGAATCAATAAATTATACACTTACACACTTAAAAGACCATGGCACATATGTATATCATGCGGTGTTTGACATGATAGGGCCTTCTTTCCCGTGTTGGTTTGAAGTTTTTTCTTTTGAGTTCTGTATGGCTATCCACAGCCATTAGTTGGTTCCAAAGCTCTAACTGGATCATCTGTTACAATGTTGTAGTTCTATAATAACCTCTCAACTGGACTGAAAGATGTTGTATGGCGAACCGGAGACGAGACGGAGCAGGAAAGTAACGGTAAGCAAAAATATGGTCGCGTTCGTTAAGATGAACGAAAGAAAGCGAATGGATGAGTACAAAATTAAAAGCTGAAAACTAAATGAGCGGAACTGGTTATATACTTATATATGTTTTACCTTGGATTCTGCAGGTGTTGCAAATGCTTGAAAGATCTATTTGATTACCTCTCTGTCTGGAGTGGTTGTGCAAATATTGTCACCCTCCACCCCCAACAAAAACAAGGAATGATTTCTTTTTTTTTTTTTAATGCCATTTAGGTGTGTGCTTTTGTGAAATGTTTCCCATATTGTGGTATGATGAAATTAATTGCTTTTTTTTTTTT

mRNA sequence

CAGGTCTGGCAACCGTGTAAGAAGAAGAGGTCTTGATCGGAGGATTTTACAGCGAGGAAAGGGATTGCGGGGTGAAGGAGTTGTTAGAATTGGCGACAGAGATGGCTTTCAGGTCGAGCAGTCATACCAAAACGCGGGTGGGGAGATATGAGCTGGGGAGAACCCTTGGAGAGGGCAGCTTCGCGAAGGTTAAGTTCGCTCGGAATTGCGAGACAGGGGAGAACGTCGCCATTAAAATCCTCAACAAAGAGGACATTCTCAAGCACAAGATGATCGGGCAGATTAAACGTGAAATATCAACGATGAAACTCATTAGACATCCAAATGTCACTCGAATGTATGAGGTGATGGCTAGCAAAAGCAAGATATATATTGTTTTGGAATTTGTAACTGGTGGAGAACTATTCAACAAAATTGTGAGATACAGTGACTGGTTTTATCTCGTATCCATGCATGCAATTGCATGTAATGGAAGATTCAAAGAAGATGAAGCAAGAAAATATTTTCAGCAGCTTATAAATGCTGTGGATTATTGCCATAGCAGAGGCGTTTGTCATCGAGACCTAAAGCCAGAGAATTTATTATTGGATGCCAGTGGCGTTCTAAAAGTTTCAGATTTTGGATTGAGTGCTCTACCTCAACAAGTTCGAGACGATGGATTACTTCACACAACATGTGGAACACCCAATTATGTTGCTCCAGAGGTTATCAACAATAAAGGCTACTGTGGTGCAAAGGCGGACCTATGGTCATGTGGGGTTATCCTTTTTGTCCTCATGGCAGGCTATTTGCCATTTGAAGAAGCCAGTCTCGTGACATTGTATAAAAAGATATTCAAGGCCGACTTCAAATGTCCTCCATGGTTCTCCTCTAGTGCCAAGAAATTAATCAAGAGAATCCTCGATCCGAATCCGTTAACTAGAATTACAATTGCTGAGGTTCTTGAGAATGATTGGTTTAAGAAAGGATACAAGCCACCCACTTTCGAAAACGACGAAATCGTTCTTGATGACGTTGACGACATCTTTAATGAATCAGGGGTCACTCATAACCTTGTAGTCGAGAAGAGAGAAGAAGGGCTCGCATCCCCTGCATCACCTGTCACTATGAATGCATTCGAGCTCATCTCGAAATCTCAGGGTCTCAACCTTGCCACTCTGTTTGAGAAGCAAATGGGGCTATCTAAACGTGAAAAAAGTTTCACATCCCATTGTCCAGCAGATGAAATCCTTTCCAAGATAAAAAAGACAGCAGTTCCTATGGGTTTTGATGTGAAGATCAATAATTTCAAGATGAAGCTCCAAGGGGAAAAAACTGGACGCAAAGGTCATTTATCAGTTTCAACAGAGGTCGCTCCTTCGTTACATATGGTAGAGCTCCGCAAAGCTGGAGGGGATACTTTAGAATTTCACAAGTTCTATAATAACCTCTCAACTGGACTGAAAGATGTTGTATGGCGAACCGGAGACGAGACGGAGCAGGAAAGTAACGGTGTTGCAAATGCTTGAAAGATCTATTTGATTACCTCTCTGTCTGGAGTGGTTGTGCAAATATTGTCACCCTCCACCCCCAACAAAAACAAGGAATGATTTCTTTTTTTTTTTTTAATGCCATTTAGGTGTGTGCTTTTGTGAAATGTTTCCCATATTGTGGTATGATGAAATTAATTGCTTTTTTTTTTTT

Coding sequence (CDS)

ATGGCTTTCAGGTCGAGCAGTCATACCAAAACGCGGGTGGGGAGATATGAGCTGGGGAGAACCCTTGGAGAGGGCAGCTTCGCGAAGGTTAAGTTCGCTCGGAATTGCGAGACAGGGGAGAACGTCGCCATTAAAATCCTCAACAAAGAGGACATTCTCAAGCACAAGATGATCGGGCAGATTAAACGTGAAATATCAACGATGAAACTCATTAGACATCCAAATGTCACTCGAATGTATGAGGTGATGGCTAGCAAAAGCAAGATATATATTGTTTTGGAATTTGTAACTGGTGGAGAACTATTCAACAAAATTGTGAGATACAGTGACTGGTTTTATCTCGTATCCATGCATGCAATTGCATGTAATGGAAGATTCAAAGAAGATGAAGCAAGAAAATATTTTCAGCAGCTTATAAATGCTGTGGATTATTGCCATAGCAGAGGCGTTTGTCATCGAGACCTAAAGCCAGAGAATTTATTATTGGATGCCAGTGGCGTTCTAAAAGTTTCAGATTTTGGATTGAGTGCTCTACCTCAACAAGTTCGAGACGATGGATTACTTCACACAACATGTGGAACACCCAATTATGTTGCTCCAGAGGTTATCAACAATAAAGGCTACTGTGGTGCAAAGGCGGACCTATGGTCATGTGGGGTTATCCTTTTTGTCCTCATGGCAGGCTATTTGCCATTTGAAGAAGCCAGTCTCGTGACATTGTATAAAAAGATATTCAAGGCCGACTTCAAATGTCCTCCATGGTTCTCCTCTAGTGCCAAGAAATTAATCAAGAGAATCCTCGATCCGAATCCGTTAACTAGAATTACAATTGCTGAGGTTCTTGAGAATGATTGGTTTAAGAAAGGATACAAGCCACCCACTTTCGAAAACGACGAAATCGTTCTTGATGACGTTGACGACATCTTTAATGAATCAGGGGTCACTCATAACCTTGTAGTCGAGAAGAGAGAAGAAGGGCTCGCATCCCCTGCATCACCTGTCACTATGAATGCATTCGAGCTCATCTCGAAATCTCAGGGTCTCAACCTTGCCACTCTGTTTGAGAAGCAAATGGGGCTATCTAAACGTGAAAAAAGTTTCACATCCCATTGTCCAGCAGATGAAATCCTTTCCAAGATAAAAAAGACAGCAGTTCCTATGGGTTTTGATGTGAAGATCAATAATTTCAAGATGAAGCTCCAAGGGGAAAAAACTGGACGCAAAGGTCATTTATCAGTTTCAACAGAGGTCGCTCCTTCGTTACATATGGTAGAGCTCCGCAAAGCTGGAGGGGATACTTTAGAATTTCACAAGTTCTATAATAACCTCTCAACTGGACTGAAAGATGTTGTATGGCGAACCGGAGACGAGACGGAGCAGGAAAGTAACGGTGTTGCAAATGCTTGA

Protein sequence

MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEIVLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLSKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEVAPSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA
Homology
BLAST of Carg19290 vs. NCBI nr
Match: KAG7034741.1 (CBL-interacting serine/threonine-protein kinase 23 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 949.1 bits (2452), Expect = 1.4e-272
Identity = 468/468 (100.00%), Postives = 468/468 (100.00%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI
Sbjct: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEVAPS 420
           SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEVAPS
Sbjct: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEVAPS 420

Query: 421 LHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           LHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA
Sbjct: 421 LHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 468

BLAST of Carg19290 vs. NCBI nr
Match: XP_022925893.1 (CBL-interacting serine/threonine-protein kinase 23-like [Cucurbita moschata])

HSP 1 Score: 897.9 bits (2319), Expect = 3.7e-257
Identity = 451/471 (95.75%), Postives = 452/471 (95.97%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELFNK               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFNK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
           SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE   V
Sbjct: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA
Sbjct: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 456

BLAST of Carg19290 vs. NCBI nr
Match: KAG6581447.1 (CBL-interacting serine/threonine-protein kinase 23, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 893.6 bits (2308), Expect = 7.0e-256
Identity = 450/471 (95.54%), Postives = 450/471 (95.54%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLI HPNV RMYEVMASKSKIYIVLEFVTGGELFNK               I
Sbjct: 61  IKREISTMKLITHPNVIRMYEVMASKSKIYIVLEFVTGGELFNK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
           SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE   V
Sbjct: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRT DETEQESNGVANA
Sbjct: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTRDETEQESNGVANA 456

BLAST of Carg19290 vs. NCBI nr
Match: XP_023543315.1 (CBL-interacting serine/threonine-protein kinase 23-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 893.6 bits (2308), Expect = 7.0e-256
Identity = 447/471 (94.90%), Postives = 451/471 (95.75%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELFNK               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFNK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITI EVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIVEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDD+DDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDIDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
           SKR+KSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSV+TE   V
Sbjct: 361 SKRDKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVATEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA
Sbjct: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 456

BLAST of Carg19290 vs. NCBI nr
Match: XP_022977412.1 (CBL-interacting serine/threonine-protein kinase 23-like [Cucurbita maxima])

HSP 1 Score: 882.5 bits (2279), Expect = 1.6e-252
Identity = 442/471 (93.84%), Postives = 448/471 (95.12%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MA RSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MASRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELFNK               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFNK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           AC+GRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACSGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLV L
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVAL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNP TRITIAEVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPSTRITIAEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDD+DDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDIDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
           SKREKSFTSHCPADEI+SKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSV+TE   V
Sbjct: 361 SKREKSFTSHCPADEIISKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVATEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVW+TGDETEQESNGVA A
Sbjct: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWQTGDETEQESNGVAKA 456

BLAST of Carg19290 vs. ExPASy Swiss-Prot
Match: Q93VD3 (CBL-interacting serine/threonine-protein kinase 23 OS=Arabidopsis thaliana OX=3702 GN=CIPK23 PE=1 SV=1)

HSP 1 Score: 698.4 bits (1801), Expect = 5.7e-200
Identity = 348/460 (75.65%), Postives = 397/460 (86.30%), Query Frame = 0

Query: 5   SSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKRE 64
           SSS  +TRVG+YELGRTLGEG+FAKVKFARN E G+NVAIK+++KE +LK+KMI QIKRE
Sbjct: 20  SSSGGRTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKRE 79

Query: 65  ISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNG 124
           ISTMKLI+HPNV RM+EVMASK+KIY VLEFVTGGELF+K               I+ NG
Sbjct: 80  ISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDK---------------ISSNG 139

Query: 125 RFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRD 184
           R KEDEARKYFQQLINAVDYCHSRGV HRDLKPENLLLDA+G LKVSDFGLSALPQQVR+
Sbjct: 140 RLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVRE 199

Query: 185 DGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKI 244
           DGLLHTTCGTPNYVAPEVINNKGY GAKADLWSCGVILFVLMAGYLPFE+++L +LYKKI
Sbjct: 200 DGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKI 259

Query: 245 FKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEIVLDD 304
           FKA+F CPPWFS+SAKKLIKRILDPNP TRIT AEV+EN+WFKKGYK P FEN ++ LDD
Sbjct: 260 FKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWFKKGYKAPKFENADVSLDD 319

Query: 305 VDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLSKRE 364
           VD IF++SG + NLVVE+REEGL    +PVTMNAFELIS SQGLNL +LFEKQMGL KR+
Sbjct: 320 VDAIFDDSGESKNLVVERREEGL---KTPVTMNAFELISTSQGLNLGSLFEKQMGLVKRK 379

Query: 365 KSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---VAPSL 424
             FTS   A+EI++KI+  A PMGFDVK NN+KMKL GEK+GRKG L+V+TE   VAPSL
Sbjct: 380 TRFTSKSSANEIVTKIEAAAAPMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSL 439

Query: 425 HMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQE 462
           +MVE+RK+GGDTLEFHKFY NL+TGLKD+VW+T DE ++E
Sbjct: 440 YMVEMRKSGGDTLEFHKFYKNLTTGLKDIVWKTIDEEKEE 461

BLAST of Carg19290 vs. ExPASy Swiss-Prot
Match: Q6ZLP5 (CBL-interacting protein kinase 23 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK23 PE=2 SV=1)

HSP 1 Score: 655.2 bits (1689), Expect = 5.5e-187
Identity = 335/465 (72.04%), Postives = 380/465 (81.72%), Query Frame = 0

Query: 5   SSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKRE 64
           S S  +TRVGRYELGRTLGEG+FAKVKFARN ++GENVAIKIL+K+ +LKHKMI QIKRE
Sbjct: 2   SVSGGRTRVGRYELGRTLGEGTFAKVKFARNADSGENVAIKILDKDKVLKHKMIAQIKRE 61

Query: 65  ISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNG 124
           ISTMKLIRHPNV RM+EVMASK+KIYIV+E VTGGELF+K               IA  G
Sbjct: 62  ISTMKLIRHPNVIRMHEVMASKTKIYIVMELVTGGELFDK---------------IASRG 121

Query: 125 RFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRD 184
           R KED+ARKYFQQLINAVDYCHSRGV HRDLKPENLLLDASG LKVSDFGLSAL QQVR+
Sbjct: 122 RLKEDDARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDASGTLKVSDFGLSALSQQVRE 181

Query: 185 DGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKI 244
           DGLLHTTCGTPNYVAPEVINNKGY GAKADLWSCGVILFVLMAGYLPFE+++L++LYKKI
Sbjct: 182 DGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLMSLYKKI 241

Query: 245 FKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEIVLDD 304
           FKADF CP WFS+SAKKLIK+ILDPNP TRITIAE++ N+WFKKGY+PP FE  ++ LDD
Sbjct: 242 FKADFSCPSWFSTSAKKLIKKILDPNPSTRITIAELINNEWFKKGYQPPRFETADVNLDD 301

Query: 305 VDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQ-MGLSKR 364
           ++ IFNESG    LVVE+REE       P  MNAFELIS SQGLNL TLFEKQ  G  KR
Sbjct: 302 INSIFNESGDQTQLVVERREE------RPSVMNAFELISTSQGLNLGTLFEKQSQGSVKR 361

Query: 365 EKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVST---EVAPS 424
           E  F S  PA+EILSKI+  A PMGF+V+  N+K+KLQGE  GRKG L+++T   EV PS
Sbjct: 362 ETRFASRLPANEILSKIEAAAGPMGFNVQKRNYKLKLQGENPGRKGQLAIATEVFEVTPS 421

Query: 425 LHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWR------TGDETE 460
           L+MVELRK+ GDTLEFHKFY+N+S GLKDV+W+       GDE +
Sbjct: 422 LYMVELRKSNGDTLEFHKFYHNISNGLKDVMWKPESSIIAGDEIQ 445

BLAST of Carg19290 vs. ExPASy Swiss-Prot
Match: Q9MAM1 (CBL-interacting serine/threonine-protein kinase 9 OS=Arabidopsis thaliana OX=3702 GN=CIPK9 PE=1 SV=2)

HSP 1 Score: 601.3 bits (1549), Expect = 9.5e-171
Identity = 300/463 (64.79%), Postives = 370/463 (79.91%), Query Frame = 0

Query: 4   RSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKR 63
           +++  ++TRVG YE+GRTLGEGSFAKVK+A+N  TG+  AIKIL++E + +HKM+ Q+KR
Sbjct: 7   KATPASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKR 66

Query: 64  EISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACN 123
           EISTMKLI+HPNV  + EVMASK+KIYIVLE V GGELF+K               IA  
Sbjct: 67  EISTMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGELFDK---------------IAQQ 126

Query: 124 GRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVR 183
           GR KEDEAR+YFQQLINAVDYCHSRGV HRDLKPENL+LDA+GVLKVSDFGLSA  +QVR
Sbjct: 127 GRLKEDEARRYFQQLINAVDYCHSRGVYHRDLKPENLILDANGVLKVSDFGLSAFSRQVR 186

Query: 184 DDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKK 243
           +DGLLHT CGTPNYVAPEV+++KGY GA AD+WSCGVILFVLMAGYLPF+E +L+TLYK+
Sbjct: 187 EDGLLHTACGTPNYVAPEVLSDKGYDGAAADVWSCGVILFVLMAGYLPFDEPNLMTLYKR 246

Query: 244 IFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFEND--EIV 303
           I KA+F CPPWFS  AK++IKRIL+PNP+TRI+IAE+LE++WFKKGYKPP+F+ D  +I 
Sbjct: 247 ICKAEFSCPPWFSQGAKRVIKRILEPNPITRISIAELLEDEWFKKGYKPPSFDQDDEDIT 306

Query: 304 LDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLS 363
           +DDVD  F+ S     LV EK+E+       PV+MNAFELIS S   +L  LFEKQ  L 
Sbjct: 307 IDDVDAAFSNS--KECLVTEKKEK-------PVSMNAFELISSSSEFSLENLFEKQAQLV 366

Query: 364 KREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVST---EVA 423
           K+E  FTS   A EI+SK+++TA P+GF+V+ +N+K+K++G+K+GRKG LSV+T   EVA
Sbjct: 367 KKETRFTSQRSASEIMSKMEETAKPLGFNVRKDNYKIKMKGDKSGRKGQLSVATEVFEVA 426

Query: 424 PSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQE 462
           PSLH+VELRK GGDTLEFHKFY N S+GLKDVVW T    E++
Sbjct: 427 PSLHVVELRKTGGDTLEFHKFYKNFSSGLKDVVWNTDAAAEEQ 445

BLAST of Carg19290 vs. ExPASy Swiss-Prot
Match: Q10SC8 (CBL-interacting protein kinase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK9 PE=2 SV=1)

HSP 1 Score: 590.1 bits (1520), Expect = 2.2e-167
Identity = 297/454 (65.42%), Postives = 357/454 (78.63%), Query Frame = 0

Query: 11  TRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKREISTMKL 70
           TRVGRYELG+T+GEGSFAKVK AR+  TG+ +AIK+L++  +L+HKM+ QIKREISTMKL
Sbjct: 22  TRVGRYELGKTIGEGSFAKVKVARDTRTGDTLAIKVLDRNHVLRHKMVEQIKREISTMKL 81

Query: 71  IRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNGRFKEDE 130
           I+HPNV +++EVMASKSKIY+VLE+V GGELF+KIV                +GR  EDE
Sbjct: 82  IKHPNVVQLHEVMASKSKIYMVLEYVDGGELFDKIVN---------------SGRLGEDE 141

Query: 131 ARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRDDGLLHT 190
           AR+YF QLINAVDYCHSRGV HRDLKPENLLLD+ G LKVSDFGLSA   Q ++DGLLHT
Sbjct: 142 ARRYFHQLINAVDYCHSRGVYHRDLKPENLLLDSHGALKVSDFGLSAFAPQTKEDGLLHT 201

Query: 191 TCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKIFKADFK 250
            CGTPNYVAPEV+ +KGY G  AD+WSCG+ILFVLMAGYLPF++ +L+TLYK I KA   
Sbjct: 202 ACGTPNYVAPEVLADKGYDGMAADVWSCGIILFVLMAGYLPFDDPNLMTLYKLICKAKVS 261

Query: 251 CPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFE-NDEIVLDDVDDIF 310
           CP WFSS AKK IKRILDPNP TRITIA++LE+DWFKK YKPP FE  +++ LDDVD  F
Sbjct: 262 CPHWFSSGAKKFIKRILDPNPCTRITIAQILEDDWFKKDYKPPLFEQGEDVSLDDVDAAF 321

Query: 311 NESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQ-MGLSKREKSFT 370
           + S    NLV EKRE+       P +MNAF LIS+SQG NL  LFEK+ MG+ KRE SFT
Sbjct: 322 DCS--EENLVAEKREK-------PESMNAFALISRSQGFNLGNLFEKEMMGMVKRETSFT 381

Query: 371 SHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVST---EVAPSLHMVE 430
           S C   EI+SKI++   P+GF+V+  N+KMKL+G+KTGRKG+LSV+T   EVAPSLHMVE
Sbjct: 382 SQCTPQEIMSKIEEACGPLGFNVRKQNYKMKLKGDKTGRKGYLSVATEVFEVAPSLHMVE 441

Query: 431 LRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETE 460
           LRK GGDTLEFH FYNN S+ LKD+VW++  + +
Sbjct: 442 LRKTGGDTLEFHNFYNNFSSELKDIVWKSESDAK 451

BLAST of Carg19290 vs. ExPASy Swiss-Prot
Match: Q2QY53 (CBL-interacting protein kinase 32 OS=Oryza sativa subsp. japonica OX=39947 GN=CIPK32 PE=2 SV=2)

HSP 1 Score: 587.8 bits (1514), Expect = 1.1e-166
Identity = 302/462 (65.37%), Postives = 361/462 (78.14%), Query Frame = 0

Query: 5   SSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKRE 64
           S++  K RVG+YELGRT+GEG+FAKVKFAR+ ETG+ VAIKIL+KE +LKHKM+ QIKRE
Sbjct: 2   STTKVKRRVGKYELGRTIGEGTFAKVKFARDTETGDPVAIKILDKEKVLKHKMVEQIKRE 61

Query: 65  ISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNG 124
           ISTMKLI+HPNV R+YEVM SK+KIYIVLE+VTGGELF+ IV +               G
Sbjct: 62  ISTMKLIKHPNVVRIYEVMGSKTKIYIVLEYVTGGELFDTIVNH---------------G 121

Query: 125 RFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRD 184
           R +EDEAR+YFQQLINAVDYCHSRGV HRDLKPENLLLD+ G LKVSDFGLSAL QQ++D
Sbjct: 122 RMREDEARRYFQQLINAVDYCHSRGVYHRDLKPENLLLDSYGNLKVSDFGLSALSQQIKD 181

Query: 185 DGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKI 244
           DGLLHTTCGTPNYVAPEV+ ++GY GA ADLWSCGVILFVL+AGYLPFE+++L+TLYKKI
Sbjct: 182 DGLLHTTCGTPNYVAPEVLEDQGYDGAMADLWSCGVILFVLLAGYLPFEDSNLMTLYKKI 241

Query: 245 FKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTF-ENDEIVLD 304
             A+F  PPW S  AK+L+ RILDPNP+TR+TI E+LE++WFKKGYK P F E  +  LD
Sbjct: 242 SNAEFTFPPWTSFPAKRLLTRILDPNPMTRVTIPEILEDEWFKKGYKRPEFDEKYDTTLD 301

Query: 305 DVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLSKR 364
           DV  +FN+S   H  V EK+EE       P  +NAFELIS S GLNL  LF+ +    KR
Sbjct: 302 DVYAVFNDSEEHH--VTEKKEE-------PEALNAFELISMSAGLNLGNLFDSEQEF-KR 361

Query: 365 EKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---VAPS 424
           E  FTS CP  EI+ KI++ A P+GFDV+  N+K++L+  K GRKG+L+V+TE   VAPS
Sbjct: 362 ETRFTSKCPPKEIVRKIEEAAKPLGFDVQKKNYKLRLEKVKAGRKGNLNVATEILQVAPS 421

Query: 425 LHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQES 463
           LHMVE+RKA GDTLEFHKFY NLS  LKDVVW++ D   Q S
Sbjct: 422 LHMVEVRKAKGDTLEFHKFYKNLSRTLKDVVWKSDDLQNQLS 438

BLAST of Carg19290 vs. ExPASy TrEMBL
Match: A0A6J1EGI3 (Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LOC111433168 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 1.8e-257
Identity = 451/471 (95.75%), Postives = 452/471 (95.97%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELFNK               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFNK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
           SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE   V
Sbjct: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA
Sbjct: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 456

BLAST of Carg19290 vs. ExPASy TrEMBL
Match: A0A6J1IIC9 (Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC111477755 PE=3 SV=1)

HSP 1 Score: 882.5 bits (2279), Expect = 7.8e-253
Identity = 442/471 (93.84%), Postives = 448/471 (95.12%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MA RSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ
Sbjct: 1   MASRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELFNK               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFNK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           AC+GRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 ACSGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLV L
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVAL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADFKCPPWFSSSAKKLIKRILDPNP TRITIAEVLENDWFKKGYKPPTFENDEI
Sbjct: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPSTRITIAEVLENDWFKKGYKPPTFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
           VLDD+DDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL
Sbjct: 301 VLDDIDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
           SKREKSFTSHCPADEI+SKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSV+TE   V
Sbjct: 361 SKREKSFTSHCPADEIISKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVATEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNGVANA 469
           APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVW+TGDETEQESNGVA A
Sbjct: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWQTGDETEQESNGVAKA 456

BLAST of Carg19290 vs. ExPASy TrEMBL
Match: A0A1S3AZT1 (Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC103484345 PE=3 SV=1)

HSP 1 Score: 798.9 bits (2062), Expect = 1.1e-227
Identity = 399/467 (85.44%), Postives = 424/467 (90.79%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MA RSS H K RVGRYELGRTLGEGSFAKVKFARNCETGENVAIKIL+KE+ILKHKMIGQ
Sbjct: 1   MASRSSGHNKARVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILDKENILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELF+K               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFDK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           +C GR KEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLK+SDFGLSALPQ
Sbjct: 121 SCKGRLKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKISDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVI+NKGY GAKADLWSCGVILFVLMAGYLPFEE++L+ L
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVIDNKGYFGAKADLWSCGVILFVLMAGYLPFEESNLMQL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADF CPPWFSSSAKKLIKRILDPNPLTRITIAE+LENDWFKKGYK P+FENDEI
Sbjct: 241 YKKIFKADFTCPPWFSSSAKKLIKRILDPNPLTRITIAEILENDWFKKGYKAPSFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
            LD++DDIFNESG T NLVVE+RE+  AS ASPVTMNAFELISKS+GL+LATLFEKQMG+
Sbjct: 301 SLDNIDDIFNESGGTDNLVVERREDKSASLASPVTMNAFELISKSRGLDLATLFEKQMGI 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
            KRE  FTS+CPADEI+SKIK TAVP+GFDVKINNFKMKLQGEKTGRKGHLSV+TE   V
Sbjct: 361 IKRETRFTSNCPADEIISKIKNTAVPLGFDVKINNFKMKLQGEKTGRKGHLSVATEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNG 465
           APSLH+VELRKAGGDTLEF KFYNNLSTGLKD+VWR GDE EQES G
Sbjct: 421 APSLHLVELRKAGGDTLEFQKFYNNLSTGLKDIVWRYGDEVEQESKG 452

BLAST of Carg19290 vs. ExPASy TrEMBL
Match: A0A5A7U9U0 (Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009240 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.8e-226
Identity = 399/468 (85.26%), Postives = 424/468 (90.60%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MA RSS H K RVGRYELGRTLGEGSFAKVKFARNCETGENVAIKIL+KE+ILKHKMIGQ
Sbjct: 1   MASRSSGHNKARVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILDKENILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELF+K               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFDK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           +C GR KEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLK+SDFGLSALPQ
Sbjct: 121 SCKGRLKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKISDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVRDDGLLHTTCGTPNYVAPEVI+NKGY GAKADLWSCGVILFVLMAGYLPFEE++L+ L
Sbjct: 181 QVRDDGLLHTTCGTPNYVAPEVIDNKGYFGAKADLWSCGVILFVLMAGYLPFEESNLMQL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADF CPPWFSSSAKKLIKRILDPNPLTRITIAE+LENDWFKKGYK P+FENDEI
Sbjct: 241 YKKIFKADFTCPPWFSSSAKKLIKRILDPNPLTRITIAEILENDWFKKGYKAPSFENDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQM-G 360
            LD++DDIFNESG T NLVVE+RE+  AS ASPVTMNAFELISKS+GL+LATLFEKQM G
Sbjct: 301 SLDNIDDIFNESGGTDNLVVERREDKSASLASPVTMNAFELISKSRGLDLATLFEKQMQG 360

Query: 361 LSKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE--- 420
           + KRE  FTS+CPADEI+SKIK TAVP+GFDVKINNFKMKLQGEKTGRKGHLSV+TE   
Sbjct: 361 IIKRETRFTSNCPADEIISKIKNTAVPLGFDVKINNFKMKLQGEKTGRKGHLSVATEIFQ 420

Query: 421 VAPSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNG 465
           VAPSLH+VELRKAGGDTLEF KFYNNLSTGLKD+VWR GDE EQES G
Sbjct: 421 VAPSLHLVELRKAGGDTLEFQKFYNNLSTGLKDIVWRYGDEVEQESKG 453

BLAST of Carg19290 vs. ExPASy TrEMBL
Match: A0A0A0KM22 (Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6G524600 PE=3 SV=1)

HSP 1 Score: 792.3 bits (2045), Expect = 1.1e-225
Identity = 398/467 (85.22%), Postives = 422/467 (90.36%), Query Frame = 0

Query: 1   MAFRSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQ 60
           MA RSS H+KTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKIL+KE ILKHKMIGQ
Sbjct: 1   MASRSSGHSKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILDKERILKHKMIGQ 60

Query: 61  IKREISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAI 120
           IKREISTMKLIRHPNV RMYEVMASK+KIYIVLEFVTGGELF+K               I
Sbjct: 61  IKREISTMKLIRHPNVIRMYEVMASKTKIYIVLEFVTGGELFDK---------------I 120

Query: 121 ACNGRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180
           +C GR KEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ
Sbjct: 121 SCKGRLKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQ 180

Query: 181 QVRDDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTL 240
           QVR DGLLHTTCGTPNYVAPEVI+NKGY GAKADLWSCGVILFVLMAGYLPFEE++L+ L
Sbjct: 181 QVRGDGLLHTTCGTPNYVAPEVIDNKGYFGAKADLWSCGVILFVLMAGYLPFEESNLMQL 240

Query: 241 YKKIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEI 300
           YKKIFKADF CPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYK P+F+NDEI
Sbjct: 241 YKKIFKADFTCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKAPSFKNDEI 300

Query: 301 VLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGL 360
            LD++DDIFNES  T +LVVE+RE+  AS ASPVTMNAFELISKS+GLNLATLFEKQMG+
Sbjct: 301 SLDNIDDIFNESEATDSLVVERREDRSASLASPVTMNAFELISKSRGLNLATLFEKQMGI 360

Query: 361 SKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---V 420
            KRE  FTS+CPADEI+SKIK TAVP+GFDVKINNFKMKLQGEKTGRKGHLSV+TE   V
Sbjct: 361 IKRETRFTSNCPADEIISKIKNTAVPLGFDVKINNFKMKLQGEKTGRKGHLSVATEIFQV 420

Query: 421 APSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQESNG 465
           APSLHMVELRKAGGDTLEF  FYNNLSTGLKD+VW+ GDE EQES G
Sbjct: 421 APSLHMVELRKAGGDTLEFQMFYNNLSTGLKDIVWKYGDEVEQESKG 452

BLAST of Carg19290 vs. TAIR 10
Match: AT1G30270.1 (CBL-interacting protein kinase 23 )

HSP 1 Score: 698.4 bits (1801), Expect = 4.0e-201
Identity = 348/460 (75.65%), Postives = 397/460 (86.30%), Query Frame = 0

Query: 5   SSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKRE 64
           SSS  +TRVG+YELGRTLGEG+FAKVKFARN E G+NVAIK+++KE +LK+KMI QIKRE
Sbjct: 20  SSSGGRTRVGKYELGRTLGEGTFAKVKFARNVENGDNVAIKVIDKEKVLKNKMIAQIKRE 79

Query: 65  ISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNG 124
           ISTMKLI+HPNV RM+EVMASK+KIY VLEFVTGGELF+K               I+ NG
Sbjct: 80  ISTMKLIKHPNVIRMFEVMASKTKIYFVLEFVTGGELFDK---------------ISSNG 139

Query: 125 RFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRD 184
           R KEDEARKYFQQLINAVDYCHSRGV HRDLKPENLLLDA+G LKVSDFGLSALPQQVR+
Sbjct: 140 RLKEDEARKYFQQLINAVDYCHSRGVYHRDLKPENLLLDANGALKVSDFGLSALPQQVRE 199

Query: 185 DGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKI 244
           DGLLHTTCGTPNYVAPEVINNKGY GAKADLWSCGVILFVLMAGYLPFE+++L +LYKKI
Sbjct: 200 DGLLHTTCGTPNYVAPEVINNKGYDGAKADLWSCGVILFVLMAGYLPFEDSNLTSLYKKI 259

Query: 245 FKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFENDEIVLDD 304
           FKA+F CPPWFS+SAKKLIKRILDPNP TRIT AEV+EN+WFKKGYK P FEN ++ LDD
Sbjct: 260 FKAEFTCPPWFSASAKKLIKRILDPNPATRITFAEVIENEWFKKGYKAPKFENADVSLDD 319

Query: 305 VDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLSKRE 364
           VD IF++SG + NLVVE+REEGL    +PVTMNAFELIS SQGLNL +LFEKQMGL KR+
Sbjct: 320 VDAIFDDSGESKNLVVERREEGL---KTPVTMNAFELISTSQGLNLGSLFEKQMGLVKRK 379

Query: 365 KSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---VAPSL 424
             FTS   A+EI++KI+  A PMGFDVK NN+KMKL GEK+GRKG L+V+TE   VAPSL
Sbjct: 380 TRFTSKSSANEIVTKIEAAAAPMGFDVKTNNYKMKLTGEKSGRKGQLAVATEVFQVAPSL 439

Query: 425 HMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQE 462
           +MVE+RK+GGDTLEFHKFY NL+TGLKD+VW+T DE ++E
Sbjct: 440 YMVEMRKSGGDTLEFHKFYKNLTTGLKDIVWKTIDEEKEE 461

BLAST of Carg19290 vs. TAIR 10
Match: AT1G01140.1 (CBL-interacting protein kinase 9 )

HSP 1 Score: 601.3 bits (1549), Expect = 6.7e-172
Identity = 300/463 (64.79%), Postives = 370/463 (79.91%), Query Frame = 0

Query: 4   RSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKR 63
           +++  ++TRVG YE+GRTLGEGSFAKVK+A+N  TG+  AIKIL++E + +HKM+ Q+KR
Sbjct: 7   KATPASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKR 66

Query: 64  EISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACN 123
           EISTMKLI+HPNV  + EVMASK+KIYIVLE V GGELF+K               IA  
Sbjct: 67  EISTMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGELFDK---------------IAQQ 126

Query: 124 GRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVR 183
           GR KEDEAR+YFQQLINAVDYCHSRGV HRDLKPENL+LDA+GVLKVSDFGLSA  +QVR
Sbjct: 127 GRLKEDEARRYFQQLINAVDYCHSRGVYHRDLKPENLILDANGVLKVSDFGLSAFSRQVR 186

Query: 184 DDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKK 243
           +DGLLHT CGTPNYVAPEV+++KGY GA AD+WSCGVILFVLMAGYLPF+E +L+TLYK+
Sbjct: 187 EDGLLHTACGTPNYVAPEVLSDKGYDGAAADVWSCGVILFVLMAGYLPFDEPNLMTLYKR 246

Query: 244 IFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFEND--EIV 303
           I KA+F CPPWFS  AK++IKRIL+PNP+TRI+IAE+LE++WFKKGYKPP+F+ D  +I 
Sbjct: 247 ICKAEFSCPPWFSQGAKRVIKRILEPNPITRISIAELLEDEWFKKGYKPPSFDQDDEDIT 306

Query: 304 LDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLS 363
           +DDVD  F+ S     LV EK+E+       PV+MNAFELIS S   +L  LFEKQ  L 
Sbjct: 307 IDDVDAAFSNS--KECLVTEKKEK-------PVSMNAFELISSSSEFSLENLFEKQAQLV 366

Query: 364 KREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVST---EVA 423
           K+E  FTS   A EI+SK+++TA P+GF+V+ +N+K+K++G+K+GRKG LSV+T   EVA
Sbjct: 367 KKETRFTSQRSASEIMSKMEETAKPLGFNVRKDNYKIKMKGDKSGRKGQLSVATEVFEVA 426

Query: 424 PSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQE 462
           PSLH+VELRK GGDTLEFHKFY N S+GLKDVVW T    E++
Sbjct: 427 PSLHVVELRKTGGDTLEFHKFYKNFSSGLKDVVWNTDAAAEEQ 445

BLAST of Carg19290 vs. TAIR 10
Match: AT1G01140.2 (CBL-interacting protein kinase 9 )

HSP 1 Score: 596.3 bits (1536), Expect = 2.2e-170
Identity = 300/465 (64.52%), Postives = 370/465 (79.57%), Query Frame = 0

Query: 4   RSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKR 63
           +++  ++TRVG YE+GRTLGEGSFAKVK+A+N  TG+  AIKIL++E + +HKM+ Q+KR
Sbjct: 7   KATPASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKR 66

Query: 64  EISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACN 123
           EISTMKLI+HPNV  + EVMASK+KIYIVLE V GGELF+K               IA  
Sbjct: 67  EISTMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGELFDK---------------IAQQ 126

Query: 124 GRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVR 183
           GR KEDEAR+YFQQLINAVDYCHSRGV HRDLKPENL+LDA+GVLKVSDFGLSA  +QVR
Sbjct: 127 GRLKEDEARRYFQQLINAVDYCHSRGVYHRDLKPENLILDANGVLKVSDFGLSAFSRQVR 186

Query: 184 DDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYK- 243
           +DGLLHT CGTPNYVAPEV+++KGY GA AD+WSCGVILFVLMAGYLPF+E +L+TLYK 
Sbjct: 187 EDGLLHTACGTPNYVAPEVLSDKGYDGAAADVWSCGVILFVLMAGYLPFDEPNLMTLYKR 246

Query: 244 -KIFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFEND--E 303
            +I KA+F CPPWFS  AK++IKRIL+PNP+TRI+IAE+LE++WFKKGYKPP+F+ D  +
Sbjct: 247 VRICKAEFSCPPWFSQGAKRVIKRILEPNPITRISIAELLEDEWFKKGYKPPSFDQDDED 306

Query: 304 IVLDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMG 363
           I +DDVD  F+ S     LV EK+E+       PV+MNAFELIS S   +L  LFEKQ  
Sbjct: 307 ITIDDVDAAFSNS--KECLVTEKKEK-------PVSMNAFELISSSSEFSLENLFEKQAQ 366

Query: 364 LSKREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVST---E 423
           L K+E  FTS   A EI+SK+++TA P+GF+V+ +N+K+K++G+K+GRKG LSV+T   E
Sbjct: 367 LVKKETRFTSQRSASEIMSKMEETAKPLGFNVRKDNYKIKMKGDKSGRKGQLSVATEVFE 426

Query: 424 VAPSLHMVELRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQE 462
           VAPSLH+VELRK GGDTLEFHKFY N S+GLKDVVW T    E++
Sbjct: 427 VAPSLHVVELRKTGGDTLEFHKFYKNFSSGLKDVVWNTDAAAEEQ 447

BLAST of Carg19290 vs. TAIR 10
Match: AT1G01140.3 (CBL-interacting protein kinase 9 )

HSP 1 Score: 595.5 bits (1534), Expect = 3.7e-170
Identity = 300/467 (64.24%), Postives = 370/467 (79.23%), Query Frame = 0

Query: 4   RSSSHTKTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKR 63
           +++  ++TRVG YE+GRTLGEGSFAKVK+A+N  TG+  AIKIL++E + +HKM+ Q+KR
Sbjct: 7   KATPASRTRVGNYEMGRTLGEGSFAKVKYAKNTVTGDQAAIKILDREKVFRHKMVEQLKR 66

Query: 64  EISTMKLIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACN 123
           EISTMKLI+HPNV  + EVMASK+KIYIVLE V GGELF+K               IA  
Sbjct: 67  EISTMKLIKHPNVVEIIEVMASKTKIYIVLELVNGGELFDK---------------IAQQ 126

Query: 124 GRFKEDEARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVR 183
           GR KEDEAR+YFQQLINAVDYCHSRGV HRDLKPENL+LDA+GVLKVSDFGLSA  +QVR
Sbjct: 127 GRLKEDEARRYFQQLINAVDYCHSRGVYHRDLKPENLILDANGVLKVSDFGLSAFSRQVR 186

Query: 184 DDGLLHTTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKK 243
           +DGLLHT CGTPNYVAPEV+++KGY GA AD+WSCGVILFVLMAGYLPF+E +L+TLYK+
Sbjct: 187 EDGLLHTACGTPNYVAPEVLSDKGYDGAAADVWSCGVILFVLMAGYLPFDEPNLMTLYKR 246

Query: 244 IFKADFKCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTFEND--EIV 303
           I KA+F CPPWFS  AK++IKRIL+PNP+TRI+IAE+LE++WFKKGYKPP+F+ D  +I 
Sbjct: 247 ICKAEFSCPPWFSQGAKRVIKRILEPNPITRISIAELLEDEWFKKGYKPPSFDQDDEDIT 306

Query: 304 LDDVDDIFNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLS 363
           +DDVD  F+ S     LV EK+E+       PV+MNAFELIS S   +L  LFEKQ  L 
Sbjct: 307 IDDVDAAFSNS--KECLVTEKKEK-------PVSMNAFELISSSSEFSLENLFEKQAQLV 366

Query: 364 KREKSFTSHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVST---EVA 423
           K+E  FTS   A EI+SK+++TA P+GF+V+ +N+K+K++G+K+GRKG LSV+T   EVA
Sbjct: 367 KKETRFTSQRSASEIMSKMEETAKPLGFNVRKDNYKIKMKGDKSGRKGQLSVATEVFEVA 426

Query: 424 PSLHMVELRKAGGDTLEFHK----FYNNLSTGLKDVVWRTGDETEQE 462
           PSLH+VELRK GGDTLEFHK    FY N S+GLKDVVW T    E++
Sbjct: 427 PSLHVVELRKTGGDTLEFHKVCDSFYKNFSSGLKDVVWNTDAAAEEQ 449

BLAST of Carg19290 vs. TAIR 10
Match: AT2G26980.3 (CBL-interacting protein kinase 3 )

HSP 1 Score: 567.4 bits (1461), Expect = 1.1e-161
Identity = 285/457 (62.36%), Postives = 356/457 (77.90%), Query Frame = 0

Query: 10  KTRVGRYELGRTLGEGSFAKVKFARNCETGENVAIKILNKEDILKHKMIGQIKREISTMK 69
           K RVG+YE+GRT+GEG+FAKVKFARN ETGE VA+KIL+KE +LKHKM  QI+REI+TMK
Sbjct: 8   KRRVGKYEVGRTIGEGTFAKVKFARNSETGEPVALKILDKEKVLKHKMAEQIRREIATMK 67

Query: 70  LIRHPNVTRMYEVMASKSKIYIVLEFVTGGELFNKIVRYSDWFYLVSMHAIACNGRFKED 129
           LI+HPNV ++YEVMASK+KI+I+LE+VTGGELF+KIV                +GR KED
Sbjct: 68  LIKHPNVVQLYEVMASKTKIFIILEYVTGGELFDKIVN---------------DGRMKED 127

Query: 130 EARKYFQQLINAVDYCHSRGVCHRDLKPENLLLDASGVLKVSDFGLSALPQQVRDDGLLH 189
           EAR+YFQQLI+AVDYCHSRGV HRDLKPENLLLD+ G LK+SDFGLSAL QQVRDDGLLH
Sbjct: 128 EARRYFQQLIHAVDYCHSRGVYHRDLKPENLLLDSYGNLKISDFGLSALSQQVRDDGLLH 187

Query: 190 TTCGTPNYVAPEVINNKGYCGAKADLWSCGVILFVLMAGYLPFEEASLVTLYKKIFKADF 249
           T+CGTPNYVAPEV+N++GY GA AD+WSCGV+L+VL+AGYLPF++++L+ LYKKI   +F
Sbjct: 188 TSCGTPNYVAPEVLNDRGYDGATADMWSCGVVLYVLLAGYLPFDDSNLMNLYKKISSGEF 247

Query: 250 KCPPWFSSSAKKLIKRILDPNPLTRITIAEVLENDWFKKGYKPPTF-ENDEIVLDDVDDI 309
            CPPW S  A KLI RILDPNP+TR+T  EV E++WFKK YKPP F E D+  +DD+D +
Sbjct: 248 NCPPWLSLGAMKLITRILDPNPMTRVTPQEVFEDEWFKKDYKPPVFEERDDSNMDDIDAV 307

Query: 310 FNESGVTHNLVVEKREEGLASPASPVTMNAFELISKSQGLNLATLFEKQMGLSKREKSFT 369
           F +S    +LV EKREE       P  +NAFE+IS S+GLNL  LF+ +    KRE   T
Sbjct: 308 FKDS--EEHLVTEKREE------QPAAINAFEIISMSRGLNLENLFDPEQEF-KRETRIT 367

Query: 370 SHCPADEILSKIKKTAVPMGFDVKINNFKMKLQGEKTGRKGHLSVSTE---VAPSLHMVE 429
               A+EI+ KI++ A P+GFDV+  N+KM+L+  K GRKG+L+V+TE   VAPSLHMV+
Sbjct: 368 LRGGANEIIEKIEEAAKPLGFDVQKKNYKMRLENVKAGRKGNLNVATEIFQVAPSLHMVQ 427

Query: 430 LRKAGGDTLEFHKFYNNLSTGLKDVVWRTGDETEQES 463
           + K+ GDTLEFHKFY  LS  L+ VVW T +E ++E+
Sbjct: 428 VSKSKGDTLEFHKFYKKLSNSLEQVVW-TNNEVKKET 439

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
KAG7034741.11.4e-272100.00CBL-interacting serine/threonine-protein kinase 23 [Cucurbita argyrosperma subsp... [more]
XP_022925893.13.7e-25795.75CBL-interacting serine/threonine-protein kinase 23-like [Cucurbita moschata][more]
KAG6581447.17.0e-25695.54CBL-interacting serine/threonine-protein kinase 23, partial [Cucurbita argyrospe... [more]
XP_023543315.17.0e-25694.90CBL-interacting serine/threonine-protein kinase 23-like [Cucurbita pepo subsp. p... [more]
XP_022977412.11.6e-25293.84CBL-interacting serine/threonine-protein kinase 23-like [Cucurbita maxima][more]
Match NameE-valueIdentityDescription
Q93VD35.7e-20075.65CBL-interacting serine/threonine-protein kinase 23 OS=Arabidopsis thaliana OX=37... [more]
Q6ZLP55.5e-18772.04CBL-interacting protein kinase 23 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Q9MAM19.5e-17164.79CBL-interacting serine/threonine-protein kinase 9 OS=Arabidopsis thaliana OX=370... [more]
Q10SC82.2e-16765.42CBL-interacting protein kinase 9 OS=Oryza sativa subsp. japonica OX=39947 GN=CIP... [more]
Q2QY531.1e-16665.37CBL-interacting protein kinase 32 OS=Oryza sativa subsp. japonica OX=39947 GN=CI... [more]
Match NameE-valueIdentityDescription
A0A6J1EGI31.8e-25795.75Non-specific serine/threonine protein kinase OS=Cucurbita moschata OX=3662 GN=LO... [more]
A0A6J1IIC97.8e-25393.84Non-specific serine/threonine protein kinase OS=Cucurbita maxima OX=3661 GN=LOC1... [more]
A0A1S3AZT11.1e-22785.44Non-specific serine/threonine protein kinase OS=Cucumis melo OX=3656 GN=LOC10348... [more]
A0A5A7U9U02.8e-22685.26Non-specific serine/threonine protein kinase OS=Cucumis melo var. makuwa OX=1194... [more]
A0A0A0KM221.1e-22585.22Non-specific serine/threonine protein kinase OS=Cucumis sativus OX=3659 GN=Csa_6... [more]
Match NameE-valueIdentityDescription
AT1G30270.14.0e-20175.65CBL-interacting protein kinase 23 [more]
AT1G01140.16.7e-17264.79CBL-interacting protein kinase 9 [more]
AT1G01140.22.2e-17064.52CBL-interacting protein kinase 9 [more]
AT1G01140.33.7e-17064.24CBL-interacting protein kinase 9 [more]
AT2G26980.31.1e-16162.36CBL-interacting protein kinase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 16..286
e-value: 2.0E-95
score: 333.0
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 16..286
e-value: 1.2E-70
score: 237.9
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 16..286
score: 50.661217
IPR004041NAF domainPFAMPF03822NAFcoord: 333..392
e-value: 3.5E-18
score: 65.5
NoneNo IPR availableGENE3D3.30.200.20Phosphorylase Kinase; domain 1coord: 5..95
e-value: 1.4E-33
score: 116.9
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 116..293
e-value: 5.4E-60
score: 204.0
NoneNo IPR availableGENE3D3.30.310.80Kinase associated domain 1, KA1coord: 331..453
e-value: 4.3E-49
score: 167.7
NoneNo IPR availablePIRSRPIRSR037921-2PIRSR037921-2coord: 20..236
e-value: 2.6E-11
score: 41.2
NoneNo IPR availablePIRSRPIRSR037014-1PIRSR037014-1coord: 9..287
e-value: 4.9E-73
score: 244.6
NoneNo IPR availablePIRSRPIRSR037993-2PIRSR037993-2coord: 15..302
e-value: 2.6E-34
score: 116.4
NoneNo IPR availablePIRSRPIRSR000606-51PIRSR000606-51coord: 8..278
e-value: 2.9E-51
score: 172.5
NoneNo IPR availablePIRSRPIRSR038172-1PIRSR038172-1coord: 14..224
e-value: 3.2E-22
score: 76.5
NoneNo IPR availablePIRSRPIRSR000620-2PIRSR000620-2coord: 17..226
e-value: 1.2E-9
score: 35.5
NoneNo IPR availablePANTHERPTHR24343SERINE/THREONINE KINASEcoord: 9..458
NoneNo IPR availablePANTHERPTHR24343:SF443NON-SPECIFIC SERINE/THREONINE PROTEIN KINASEcoord: 9..458
NoneNo IPR availableCDDcd14663STKc_SnRK3coord: 15..285
e-value: 1.87262E-166
score: 467.267
NoneNo IPR availableCDDcd12195CIPK_Ccoord: 337..449
e-value: 1.73387E-43
score: 147.334
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 150..162
IPR017441Protein kinase, ATP binding sitePROSITEPS00107PROTEIN_KINASE_ATPcoord: 22..45
IPR018451NAF/FISL domainPROSITEPS50816NAFcoord: 331..355
score: 12.291406
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 13..295

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
Carg19290-RACarg19290-RAmRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035556 intracellular signal transduction
biological_process GO:1990573 potassium ion import across plasma membrane
biological_process GO:0006468 protein phosphorylation
biological_process GO:0010119 regulation of stomatal movement
biological_process GO:0007584 response to nutrient
biological_process GO:0009414 response to water deprivation
biological_process GO:0007165 signal transduction
cellular_component GO:0005829 cytosol
cellular_component GO:0005634 nucleus
cellular_component GO:0005886 plasma membrane
molecular_function GO:0005524 ATP binding
molecular_function GO:0106310 protein serine kinase activity
molecular_function GO:0004674 protein serine/threonine kinase activity
molecular_function GO:0004712 protein serine/threonine/tyrosine kinase activity
molecular_function GO:0004672 protein kinase activity