Carg19269 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19269
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	gibberellin 2-beta-dioxygenase
Location	Carg_Chr03: 9704115 .. 9705222 (+)
RNA-Seq Expression	Carg19269
Synteny	Carg19269

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGTTGTTCTATCTCAGCCAGCGTTGGATTTGGAGAAATATTCTCTTCTCCGATCAACGGGCAAACCCTCCGCCTCCGCCGCCGATATTCCGGAAATTGACCTTTCAGACCCCAACGCAAAGTCCCACGTCGTCAAAGCCTGTGAAGAATTCGGGTTCTTCAAGCTGGTAAACCACCGGGTTCCCGTCGAGTTGATGACCAAACTCGAAGATGAATCCCTCTGTTTTTTCAAGCTTTCTCAGTCCGAGAAAGATAAGGCTCGCCCGCCTGACCCTTTGGGCTATGACTCCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATCGAATATCTCCTTCTCAACGCTAATCCTCTCCCCCTTTTCTCCCAACAGTCCGATTCCTTCCTGTAAGTTGCCAAAATTTAAAGCAATCTGTTTCGGAATTACAGAGTTCAAGCCTCTGTTCTTAGCCTTTTTAAGATTTTGATTTTGGTTCTGATAATTTTAATGTTTTTGTAGCTCTGTGGCGGCGGAGTATGTGACGGCGGTGAAGAAATTGAGCGGCGAAGTGGTGGAATTAATAACAGAAGGATTAAAAATCGAGCCAAAAAATGCGATAAGCAGGTTTATAAACGACGAGAATGCTGATTGTTATTTCAGAGTGAACCATTACCCGCCATGTCCAGAAATGCAAGCTGTTGGAGGACGAAACATGATCGGATTCGGGGAACATACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCAACCGGCTTGCAAATCTGCCGGAGAGACGGCGTGTGGGTCTCAGTGCCGCCAGATACGACGGCGTTCTTTGTCAATGTCGGCGATGCGCTGCAGGTGATGACCAACGGGAGATTCAAAAGCGTGAAGCATAAAGTGGTGGCGGATTTCAGTAAAGAGAGGGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTCAGCCATTGTCGCAGGTTTTAGCAGAGGGGGAAGAGAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTTTAAGACCAAATTGGCTGATAACAGGCTCGGTGAGTTTGAAAAGAGTCAAAATTCTACGTTTTAA

mRNA sequence

ATGGTTGTTCTATCTCAGCCAGCGTTGGATTTGGAGAAATATTCTCTTCTCCGATCAACGGGCAAACCCTCCGCCTCCGCCGCCGATATTCCGGAAATTGACCTTTCAGACCCCAACGCAAAGTCCCACGTCGTCAAAGCCTGTGAAGAATTCGGGTTCTTCAAGCTGGTAAACCACCGGGTTCCCGTCGAGTTGATGACCAAACTCGAAGATGAATCCCTCTGTTTTTTCAAGCTTTCTCAGTCCGAGAAAGATAAGGCTCGCCCGCCTGACCCTTTGGGCTATGACTCCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATCGAATATCTCCTTCTCAACGCTAATCCTCTCCCCCTTTTCTCCCAACAGTCCGATTCCTTCCTCTCTGTGGCGGCGGAGTATGTGACGGCGGTGAAGAAATTGAGCGGCGAAGTGGTGGAATTAATAACAGAAGGATTAAAAATCGAGCCAAAAAATGCGATAAGCAGGTTTATAAACGACGAGAATGCTGATTGTTATTTCAGAGTGAACCATTACCCGCCATGTCCAGAAATGCAAGCTGTTGGAGGACGAAACATGATCGGATTCGGGGAACATACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCAACCGGCTTGCAAATCTGCCGGAGAGACGGCGTGTGGGTCTCAGTGCCGCCAGATACGACGGCGTTCTTTGTCAATGTCGGCGATGCGCTGCAGGTGATGACCAACGGGAGATTCAAAAGCGTGAAGCATAAAGTGGTGGCGGATTTCAGTAAAGAGAGGGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTCAGCCATTGTCGCAGGTTTTAGCAGAGGGGGAAGAGAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTTTAAGACCAAATTGGCTGATAACAGGCTCGGTGAGTTTGAAAAGAGTCAAAATTCTACGTTTTAA

Coding sequence (CDS)

ATGGTTGTTCTATCTCAGCCAGCGTTGGATTTGGAGAAATATTCTCTTCTCCGATCAACGGGCAAACCCTCCGCCTCCGCCGCCGATATTCCGGAAATTGACCTTTCAGACCCCAACGCAAAGTCCCACGTCGTCAAAGCCTGTGAAGAATTCGGGTTCTTCAAGCTGGTAAACCACCGGGTTCCCGTCGAGTTGATGACCAAACTCGAAGATGAATCCCTCTGTTTTTTCAAGCTTTCTCAGTCCGAGAAAGATAAGGCTCGCCCGCCTGACCCTTTGGGCTATGACTCCAAAAATATCGGCTCTAATGGCGATAAGGGCTGGATCGAATATCTCCTTCTCAACGCTAATCCTCTCCCCCTTTTCTCCCAACAGTCCGATTCCTTCCTCTCTGTGGCGGCGGAGTATGTGACGGCGGTGAAGAAATTGAGCGGCGAAGTGGTGGAATTAATAACAGAAGGATTAAAAATCGAGCCAAAAAATGCGATAAGCAGGTTTATAAACGACGAGAATGCTGATTGTTATTTCAGAGTGAACCATTACCCGCCATGTCCAGAAATGCAAGCTGTTGGAGGACGAAACATGATCGGATTCGGGGAACATACAGACCCACAAATCATATCACTGTTAAGATCAAACAACTCAACCGGCTTGCAAATCTGCCGGAGAGACGGCGTGTGGGTCTCAGTGCCGCCAGATACGACGGCGTTCTTTGTCAATGTCGGCGATGCGCTGCAGGTGATGACCAACGGGAGATTCAAAAGCGTGAAGCATAAAGTGGTGGCGGATTTCAGTAAAGAGAGGGTTTCGATGATTTACTTTGGGGGGCCGCCGTTGAGTGAAAAGATTCAGCCATTGTCGCAGGTTTTAGCAGAGGGGGAAGAGAGCTTGTACAAGGAATTCACCTGGTGGGAATACAAAACGGCGGCGTTTAAGACCAAATTGGCTGATAACAGGCTCGGTGAGTTTGAAAAGAGTCAAAATTCTACGTTTTAA

Protein sequence

MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKEFTWWEYKTAAFKTKLADNRLGEFEKSQNSTF

Homology

[Close]

BLAST of Carg19269 vs. NCBI nr
Match: KAG7034720.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 672.5 bits (1734), Expect = 1.8e-189
Identity = 331/331 (100.00%), Postives = 331/331 (100.00%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH
Sbjct: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF
Sbjct: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 331

[Close]

BLAST of Carg19269 vs. NCBI nr
Match: KAG6581428.1 (Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 669.8 bits (1727), Expect = 1.2e-188
Identity = 330/331 (99.70%), Postives = 330/331 (99.70%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH
Sbjct: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF
Sbjct: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 331

[Close]

BLAST of Carg19269 vs. NCBI nr
Match: XP_022925964.1 (gibberellin 2-beta-dioxygenase [Cucurbita moschata])

HSP 1 Score: 659.1 bits (1699), Expect = 2.1e-185
Identity = 323/331 (97.58%), Postives = 327/331 (98.79%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQP LDLEKYS+LRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPELDLEKYSVLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           +FSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDE ADCYFRVNH
Sbjct: 121 IFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKT+LADNRLG FEKSQNSTF
Sbjct: 301 FTWWEYKTAAFKTRLADNRLGAFEKSQNSTF 331

[Close]

BLAST of Carg19269 vs. NCBI nr
Match: CBL59329.1 (Gibberellin 2-oxidase [Cucurbita maxima])

HSP 1 Score: 648.3 bits (1671), Expect = 3.6e-182
Identity = 320/331 (96.68%), Postives = 322/331 (97.28%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           LFSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDE ADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKT+LAD RLG FEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of Carg19269 vs. NCBI nr
Match: XP_022977872.1 (gibberellin 2-beta-dioxygenase [Cucurbita maxima])

HSP 1 Score: 646.7 bits (1667), Expect = 1.1e-181
Identity = 319/331 (96.37%), Postives = 322/331 (97.28%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           LFSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDE ADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKT+LAD RLG FEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of Carg19269 vs. ExPASy Swiss-Prot
Match: Q9XG83 (Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=1)

HSP 1 Score: 413.7 bits (1062), Expect = 2.0e-114
Identity = 205/333 (61.56%), Postives = 253/333 (75.98%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPAL+  ++ LL+   K +     IP +DL+ P+AK+ +V AC +FGFFKLVNH 
Sbjct: 1   MVVLSQPALN--QFFLLKPF-KSTPLFTGIPVVDLTHPDAKNLIVNACRDFGFFKLVNHG 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANP-- 120
           VP+ELM  LE+E+L FFK SQSEKD+A PPDP GY SK IG NGD GW+EYLLLN NP  
Sbjct: 61  VPLELMANLENEALRFFKKSQSEKDRAGPPDPFGYGSKRIGPNGDVGWVEYLLLNTNPDV 120

Query: 121 -----LPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENAD 180
                L +F +    F +V   Y+TAVK +   V+EL+ EGL I  +N +SR + DE +D
Sbjct: 121 ISPKSLCIFRENPHHFRAVVENYITAVKNMCYAVLELMAEGLGIRQRNTLSRLLKDEKSD 180

Query: 181 CYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPD 240
             FR+NHYPPCPE+QA+  RN++GFGEHTDPQIIS+LRSN+++GLQIC  DG WVSVPPD
Sbjct: 181 SCFRLNHYPPCPEVQAL-NRNLVGFGEHTDPQIISVLRSNSTSGLQICLTDGTWVSVPPD 240

Query: 241 TTAFFVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEG 300
            T+FF+NVGDALQVMTNGRFKSVKH+V+AD +K R+SMIYFGGP LSE I PL  V+ +G
Sbjct: 241 QTSFFINVGDALQVMTNGRFKSVKHRVLADTTKSRLSMIYFGGPALSENIAPLPSVMLKG 300

Query: 301 EESLYKEFTWWEYKTAAFKTKLADNRLGEFEKS 327
           EE LYKEFTW EYK AA+ ++LADNRL  F+KS
Sbjct: 301 EECLYKEFTWCEYKKAAYTSRLADNRLAPFQKS 329

[Close]

BLAST of Carg19269 vs. ExPASy Swiss-Prot
Match: Q9SQ80 (Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1)

HSP 1 Score: 381.3 bits (978), Expect = 1.1e-104
Identity = 181/328 (55.18%), Postives = 250/328 (76.22%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MV+LS+P    E+Y+ +R+   P   ++ IP +DLS P+AK+ +VKACE+FGFFK++NH 
Sbjct: 1   MVLLSKPT--SEQYTYVRN-NMPITFSSSIPLVDLSKPDAKTLIVKACEDFGFFKVINHG 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANP-- 120
           +P++ +++LE E+  FF L Q+EK+KA P +P GY +K IG NGD GWIEYLLL  N   
Sbjct: 61  IPLDAISQLESEAFKFFSLPQTEKEKAGPANPFGYGNKRIGLNGDIGWIEYLLLTTNQDH 120

Query: 121 -LPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFR 180
              L+ +  D F  +  +Y  A++ ++ E+++L+ EGLKI+PKN  S+ + D+ +DC FR
Sbjct: 121 NFSLYGEDIDKFRGLLKDYKCAMRNMACEILDLMAEGLKIQPKNVFSKLVMDKQSDCLFR 180

Query: 181 VNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAF 240
           VNHYP CPE+ A+ G N+IGFGEHTDPQIIS+LRSNN++G QI  RDG W+SVPPD ++F
Sbjct: 181 VNHYPACPEL-AINGENLIGFGEHTDPQIISILRSNNTSGFQISLRDGSWISVPPDHSSF 240

Query: 241 FVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESL 300
           F+NVGD+LQVMTNGRFKSV+H+V+A+    R+SMIYF GPPLSEKI PL   L +G+ESL
Sbjct: 241 FINVGDSLQVMTNGRFKSVRHRVLANGIDPRLSMIYFCGPPLSEKIAPLPS-LMKGKESL 300

Query: 301 YKEFTWWEYKTAAFKTKLADNRLGEFEK 326
           YKEFTW+EYK++ + ++LADNRLG +E+
Sbjct: 301 YKEFTWFEYKSSTYGSRLADNRLGNYER 323

[Close]

BLAST of Carg19269 vs. ExPASy Swiss-Prot
Match: Q9XFR9 (Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 SV=1)

HSP 1 Score: 365.5 bits (937), Expect = 6.2e-100
Identity = 185/334 (55.39%), Postives = 241/334 (72.16%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGK--PSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60
           MVVL QP + L+ +  L  T K  P  ++  IP ++L+DP AK+ +VKACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L QS K++A PP+P GY +K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEN 180
                    +F Q    F     EY+  +K++S +V+E++ E L IEP++ +S+ + DE 
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVP 240
           +D   R+NHYP   E         +GFGEHTDPQIIS+LRSNN+ GLQIC +DG WV+VP
Sbjct: 181 SDSCLRLNHYPAAEE--EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDTTAFFVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLA 300
           PD ++FF+NVGDALQVMTNGRFKSVKH+V+AD  + R+SMIYFGGPPLS+KI PL  ++ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAFKTKLADNRLGEFEK 326
           E ++ LYKEFTW +YK++A+K+KL D RLG FEK
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Carg19269 vs. ExPASy Swiss-Prot
Match: Q8LEA2 (Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 SV=2)

HSP 1 Score: 345.5 bits (885), Expect = 6.6e-94
Identity = 166/312 (53.21%), Postives = 229/312 (73.40%), Query Frame = 0


Query: 23  PSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSQS 82
           P +  + IP ID+SDP +K  +VKACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNAN------PL-PLFSQQSDSFLSVAAE 142
           EK +     P GY +  IG NGD GW+EYLL+NAN      PL P   +   +F +   E
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNHYPPCP--EMQAVGGR 202
           Y T+V+K++ +V+E IT+GL I+P+N +S+ ++D+N D   R+NHYPPCP    +  GG+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIIS+LRSNN++GLQI   DG W+SVPPD T+FF NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKEFTWWEYKTAAFKT 322
           KSV+H+V+A+  K RVSMIYF GP L+++I PL+ ++   +E LY+EFTW EYK + + +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 KLADNRLGEFEK 326
           +L+DNRL +FE+
Sbjct: 311 RLSDNRLQQFER 321

[Close]

BLAST of Carg19269 vs. ExPASy Swiss-Prot
Match: O64692 (Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 SV=1)

HSP 1 Score: 344.7 bits (883), Expect = 1.1e-93
Identity = 175/333 (52.55%), Postives = 227/333 (68.17%), Query Frame = 0


Query: 1   MVVLSQPA-LDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNH 60
           MV++ QPA  D   Y   +   +P      IP IDL+D +AK+ +VKACEEFGFFK++NH
Sbjct: 1   MVIVLQPASFDSNLYVNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVINH 60

Query: 61  RVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNAN-- 120
            V  +L+T+LE E++ FF L  S KDKA PPDP GY +K IG NGD GW+EY+LLNAN  
Sbjct: 61  GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLC 120

Query: 121 -----PLPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENA 180
                   +F      F     EY+  +K++S + +E++ E LKIEPK  +SR +  + +
Sbjct: 121 LESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKES 180

Query: 181 DCYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPP 240
           D   R+NHY   PE +    +  IGFGEHTDPQ+ISLLRSN++ GLQIC +DG WV V P
Sbjct: 181 DSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTP 240

Query: 241 DTTAFFVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAE 300
           D ++FFV VGD LQVMTNGRFKSVKH+VV +  + R+SMIYF GPPLSEKI PLS ++ +
Sbjct: 241 DHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPK 300

Query: 301 GEESLYKEFTWWEYKTAAFKTKLADNRLGEFEK 326
            ++ LY EFTW +YK +A+KTKL D RLG FEK
Sbjct: 301 QDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Carg19269 vs. ExPASy TrEMBL
Match: A0A6J1EDN7 (gibberellin 2-beta-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111433225 PE=3 SV=1)

HSP 1 Score: 659.1 bits (1699), Expect = 1.0e-185
Identity = 323/331 (97.58%), Postives = 327/331 (98.79%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQP LDLEKYS+LRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPELDLEKYSVLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           +FSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDE ADCYFRVNH
Sbjct: 121 IFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKT+LADNRLG FEKSQNSTF
Sbjct: 301 FTWWEYKTAAFKTRLADNRLGAFEKSQNSTF 331

[Close]

BLAST of Carg19269 vs. ExPASy TrEMBL
Match: I0J1J0 (Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1)

HSP 1 Score: 648.3 bits (1671), Expect = 1.8e-182
Identity = 320/331 (96.68%), Postives = 322/331 (97.28%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           LFSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDE ADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKT+LAD RLG FEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of Carg19269 vs. ExPASy TrEMBL
Match: A0A6J1IJN1 (gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 SV=1)

HSP 1 Score: 646.7 bits (1667), Expect = 5.1e-182
Identity = 319/331 (96.37%), Postives = 322/331 (97.28%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPALDLEKYSLLRSTGKP+ASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALDLEKYSLLRSTGKPAASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGY SKNIGSNGDKGWIEYLLLNANPLP
Sbjct: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYGSKNIGSNGDKGWIEYLLLNANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           LFSQQSDSFLS AAEYVTAVKKLSGEVVELITEGLKIEPKNAISR INDE ADCYFRVNH
Sbjct: 121 LFSQQSDSFLSAAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRLINDEKADCYFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQAV GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN
Sbjct: 181 YPPCPEMQAVSGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVVAD SKERVSMIYFGGPPLSEKI+PLSQVLAEGEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVADSSKERVSMIYFGGPPLSEKIRPLSQVLAEGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNSTF 332
           FTWWEYKTAAFKT+LAD RLG FEKSQ STF
Sbjct: 301 FTWWEYKTAAFKTRLADYRLGAFEKSQKSTF 331

[Close]

BLAST of Carg19269 vs. ExPASy TrEMBL
Match: A0A2H4V3T7 (Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 SV=1)

HSP 1 Score: 566.2 bits (1458), Expect = 8.8e-158
Identity = 272/331 (82.18%), Postives = 302/331 (91.24%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQPAL L++YSLLR+T KP+AS  +IPEIDLSDPNA+SH+V+ACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPALGLDQYSLLRTTCKPTASFTEIPEIDLSDPNAQSHIVEACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLE+E+LCFFKLSQSEKDKARPPDPLGY  KNIGSNGDKGWIEYLLL ANPLP
Sbjct: 61  VPIELMTKLEEEALCFFKLSQSEKDKARPPDPLGYGCKNIGSNGDKGWIEYLLLTANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           +FSQ S+SFL+ A EYVTAVKKLS EV ELI EGLKIEP+NAIS+ +NDE +DC FRVNH
Sbjct: 121 IFSQNSESFLAAAKEYVTAVKKLSCEVAELIAEGLKIEPRNAISKLLNDEKSDCCFRVNH 180

Query: 181 YPPCPEMQAVG--GRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFF 240
           YPPCP++QA+G  GRNMIGFGEHTDPQIISLLRSNNSTGLQIC RDG WVSVPPD  A F
Sbjct: 181 YPPCPDLQALGGSGRNMIGFGEHTDPQIISLLRSNNSTGLQICLRDGAWVSVPPDAAAIF 240

Query: 241 VNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLY 300
           VNVGD+LQVM+NGRFKSVKHKVVAD ++ERVSMIYFGGPPLSEKI PL +VL +GEESLY
Sbjct: 241 VNVGDSLQVMSNGRFKSVKHKVVADSNRERVSMIYFGGPPLSEKIAPLPEVLGDGEESLY 300

Query: 301 KEFTWWEYKTAAFKTKLADNRLGEFEKSQNS 330
           KEFTWWEYKTAA+KT+LAD RLG FEK+QNS
Sbjct: 301 KEFTWWEYKTAAYKTRLADYRLGPFEKNQNS 331

[Close]

BLAST of Carg19269 vs. ExPASy TrEMBL
Match: A0A5A7UGB1 (Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold255G009030 PE=3 SV=1)

HSP 1 Score: 556.2 bits (1432), Expect = 9.1e-155
Identity = 272/330 (82.42%), Postives = 293/330 (88.79%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHR 60
           MVVLSQP LDL +YSLLRST KP+AS ADIPEIDLSDPNAK H+VKACEEFGFFKLVNHR
Sbjct: 1   MVVLSQPTLDLNQYSLLRSTCKPTASFADIPEIDLSDPNAKFHIVKACEEFGFFKLVNHR 60

Query: 61  VPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLP 120
           VP+ELMTKLEDESLCFFKLS+SEKDKARPP PLGY SKNIGSNGDKGWIEYLL+NANPLP
Sbjct: 61  VPIELMTKLEDESLCFFKLSESEKDKARPPHPLGYGSKNIGSNGDKGWIEYLLINANPLP 120

Query: 121 LFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNH 180
           +FS     FL  A EYVTAVKKLS EVVELI EGLK+E +NAIS+ +NDE ADC FRVNH
Sbjct: 121 IFSH---DFLRAATEYVTAVKKLSCEVVELIAEGLKMERRNAISKLLNDEKADCCFRVNH 180

Query: 181 YPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVN 240
           YPPCPEMQ + GRNMIGFGEHTDPQIIS+LRSNNSTGLQIC RDG WVSVPPD  A FVN
Sbjct: 181 YPPCPEMQGLSGRNMIGFGEHTDPQIISILRSNNSTGLQICLRDGAWVSVPPDNGALFVN 240

Query: 241 VGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKE 300
           VGDALQVMTNGRFKSVKHKVV D ++ERVSMIYFGGPPLSEKI PL ++L +GEESLYKE
Sbjct: 241 VGDALQVMTNGRFKSVKHKVVVDPNRERVSMIYFGGPPLSEKITPLPELLEDGEESLYKE 300

Query: 301 FTWWEYKTAAFKTKLADNRLGEFEKSQNST 331
           FTWWEYKTAA+K+KLAD RLG FEKS  S+
Sbjct: 301 FTWWEYKTAAYKSKLADYRLGAFEKSPISS 327

[Close]

BLAST of Carg19269 vs. TAIR 10
Match: AT1G30040.1 (gibberellin 2-oxidase )

HSP 1 Score: 365.5 bits (937), Expect = 4.4e-101
Identity = 185/334 (55.39%), Postives = 241/334 (72.16%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGK--PSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60
           MVVL QP + L+ +  L  T K  P  ++  IP ++L+DP AK+ +VKACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L QS K++A PP+P GY +K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEN 180
                    +F Q    F     EY+  +K++S +V+E++ E L IEP++ +S+ + DE 
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVP 240
           +D   R+NHYP   E         +GFGEHTDPQIIS+LRSNN+ GLQIC +DG WV+VP
Sbjct: 181 SDSCLRLNHYPAAEE--EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDTTAFFVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLA 300
           PD ++FF+NVGDALQVMTNGRFKSVKH+V+AD  + R+SMIYFGGPPLS+KI PL  ++ 
Sbjct: 241 PDHSSFFINVGDALQVMTNGRFKSVKHRVLADTRRSRISMIYFGGPPLSQKIAPLPCLVP 300

Query: 301 EGEESLYKEFTWWEYKTAAFKTKLADNRLGEFEK 326
           E ++ LYKEFTW +YK++A+K+KL D RLG FEK
Sbjct: 301 EQDDWLYKEFTWSQYKSSAYKSKLGDYRLGLFEK 331

[Close]

BLAST of Carg19269 vs. TAIR 10
Match: AT1G78440.1 (Arabidopsis thaliana gibberellin 2-oxidase 1 )

HSP 1 Score: 345.5 bits (885), Expect = 4.7e-95
Identity = 166/312 (53.21%), Postives = 229/312 (73.40%), Query Frame = 0


Query: 23  PSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNHRVPVELMTKLEDESLCFFKLSQS 82
           P +  + IP ID+SDP +K  +VKACE+FGFFK++NH V  EL++ LE E++ FF L +S
Sbjct: 11  PKSGFSLIPVIDMSDPESKHALVKACEDFGFFKVINHGVSAELVSVLEHETVDFFSLPKS 70

Query: 83  EKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNAN------PL-PLFSQQSDSFLSVAAE 142
           EK +     P GY +  IG NGD GW+EYLL+NAN      PL P   +   +F +   E
Sbjct: 71  EKTQV-AGYPFGYGNSKIGRNGDVGWVEYLLMNANHDSGSGPLFPSLLKSPGTFRNALEE 130

Query: 143 YVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNHYPPCP--EMQAVGGR 202
           Y T+V+K++ +V+E IT+GL I+P+N +S+ ++D+N D   R+NHYPPCP    +  GG+
Sbjct: 131 YTTSVRKMTFDVLEKITDGLGIKPRNTLSKLVSDQNTDSILRLNHYPPCPLSNKKTNGGK 190

Query: 203 NMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDALQVMTNGRF 262
           N+IGFGEHTDPQIIS+LRSNN++GLQI   DG W+SVPPD T+FF NVGD+LQVMTNGRF
Sbjct: 191 NVIGFGEHTDPQIISVLRSNNTSGLQINLNDGSWISVPPDHTSFFFNVGDSLQVMTNGRF 250

Query: 263 KSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKEFTWWEYKTAAFKT 322
           KSV+H+V+A+  K RVSMIYF GP L+++I PL+ ++   +E LY+EFTW EYK + + +
Sbjct: 251 KSVRHRVLANCKKSRVSMIYFAGPSLTQRIAPLTCLIDNEDERLYEEFTWSEYKNSTYNS 310

Query: 323 KLADNRLGEFEK 326
           +L+DNRL +FE+
Sbjct: 311 RLSDNRLQQFER 321

[Close]

BLAST of Carg19269 vs. TAIR 10
Match: AT2G34555.1 (gibberellin 2-oxidase 3 )

HSP 1 Score: 344.7 bits (883), Expect = 8.0e-95
Identity = 175/333 (52.55%), Postives = 227/333 (68.17%), Query Frame = 0


Query: 1   MVVLSQPA-LDLEKYSLLRSTGKPSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVNH 60
           MV++ QPA  D   Y   +   +P      IP IDL+D +AK+ +VKACEEFGFFK++NH
Sbjct: 1   MVIVLQPASFDSNLYVNPKCKPRPVL----IPVIDLTDSDAKTQIVKACEEFGFFKVINH 60

Query: 61  RVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNAN-- 120
            V  +L+T+LE E++ FF L  S KDKA PPDP GY +K IG NGD GW+EY+LLNAN  
Sbjct: 61  GVRPDLLTQLEQEAINFFALHHSLKDKAGPPDPFGYGTKRIGPNGDLGWLEYILLNANLC 120

Query: 121 -----PLPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENA 180
                   +F      F     EY+  +K++S + +E++ E LKIEPK  +SR +  + +
Sbjct: 121 LESHKTTAIFRHTPAIFREAVEEYIKEMKRMSSKFLEMVEEELKIEPKEKLSRLVKVKES 180

Query: 181 DCYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPP 240
           D   R+NHY   PE +    +  IGFGEHTDPQ+ISLLRSN++ GLQIC +DG WV V P
Sbjct: 181 DSCLRMNHY---PEKEETPVKEEIGFGEHTDPQLISLLRSNDTEGLQICVKDGTWVDVTP 240

Query: 241 DTTAFFVNVGDALQVMTNGRFKSVKHKVVADFSKERVSMIYFGGPPLSEKIQPLSQVLAE 300
           D ++FFV VGD LQVMTNGRFKSVKH+VV +  + R+SMIYF GPPLSEKI PLS ++ +
Sbjct: 241 DHSSFFVLVGDTLQVMTNGRFKSVKHRVVTNTKRSRISMIYFAGPPLSEKIAPLSCLVPK 300

Query: 301 GEESLYKEFTWWEYKTAAFKTKLADNRLGEFEK 326
            ++ LY EFTW +YK +A+KTKL D RLG FEK
Sbjct: 301 QDDCLYNEFTWSQYKLSAYKTKLGDYRLGLFEK 326

[Close]

BLAST of Carg19269 vs. TAIR 10
Match: AT1G02400.1 (gibberellin 2-oxidase 6 )

HSP 1 Score: 261.9 bits (668), Expect = 6.8e-70
Identity = 139/321 (43.30%), Postives = 198/321 (61.68%), Query Frame = 0


Query: 17  LRSTGKPSASAAD--IPEID--LSDPNAKSH-VVKACEEFGFFKLVNHRVPVELMTKLED 76
           L++TGK + S+ +   P ID  L+D +  S  +VKACE  GFFK++NH V  E++ + E 
Sbjct: 9   LQTTGKKTISSPEYNFPVIDFSLNDRSKLSEKIVKACEVNGFFKVINHGVKPEIIKRFEH 68

Query: 77  ESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANPLPLFSQQSD---- 136
           E   FF   +S+K +A P  P GY  KNIG NGD G +EYLLL+ANP  +  +       
Sbjct: 69  EGEEFFNKPESDKLRAGPASPFGYGCKNIGFNGDLGELEYLLLHANPTAVADKSETISHD 128

Query: 137 ---SFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDENADCYFRVNHYPPC 196
               F S   +Y+  V+ L+ E+++L  E L  +  + +S  I D  +D   R+NHYPP 
Sbjct: 129 DPFKFSSATNDYIRTVRDLACEIIDLTIENLWGQKSSEVSELIRDVRSDSILRLNHYPPA 188

Query: 197 PEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVPPDTTAFFVNVGDA 256
           P   A+ G   IGFGEH+DPQI+++LRSN+  GL+IC RDG+W+ +P D T FFV VGD 
Sbjct: 189 P--YALSGVGQIGFGEHSDPQILTVLRSNDVDGLEICSRDGLWIPIPSDPTCFFVLVGDC 248

Query: 257 LQVMTNGRFKSVKHKVVADFSKE-RVSMIYFGGPPLSEKIQPLSQVLAEGEESLYKEFTW 316
           LQ +TNGRF SV+H+V+A+ +K+ R+S +YF  PPL  KI PL ++++      Y  FTW
Sbjct: 249 LQALTNGRFTSVRHRVLANTAKKPRMSAMYFAAPPLEAKISPLPKMVSPENPRRYNSFTW 308

Query: 317 WEYKTAAFKTKLADNRLGEFE 325
            +YK A +  +L   RL  F+
Sbjct: 309 GDYKKATYSLRLDVPRLEFFK 327

[Close]

BLAST of Carg19269 vs. TAIR 10
Match: AT1G30040.2 (gibberellin 2-oxidase )

HSP 1 Score: 259.6 bits (662), Expect = 3.4e-69
Identity = 134/256 (52.34%), Postives = 176/256 (68.75%), Query Frame = 0


Query: 1   MVVLSQPALDLEKYSLLRSTGK--PSASAADIPEIDLSDPNAKSHVVKACEEFGFFKLVN 60
           MVVL QP + L+ +  L  T K  P  ++  IP ++L+DP AK+ +VKACEEFGFFK+VN
Sbjct: 1   MVVLPQP-VTLDNHISLIPTYKPVPVLTSHSIPVVNLADPEAKTRIVKACEEFGFFKVVN 60

Query: 61  HRVPVELMTKLEDESLCFFKLSQSEKDKARPPDPLGYDSKNIGSNGDKGWIEYLLLNANP 120
           H V  ELMT+LE E++ FF L QS K++A PP+P GY +K IG NGD GWIEYLLLNANP
Sbjct: 61  HGVRPELMTRLEQEAIGFFGLPQSLKNRAGPPEPYGYGNKRIGPNGDVGWIEYLLLNANP 120

Query: 121 -------LPLFSQQSDSFLSVAAEYVTAVKKLSGEVVELITEGLKIEPKNAISRFINDEN 180
                    +F Q    F     EY+  +K++S +V+E++ E L IEP++ +S+ + DE 
Sbjct: 121 QLSSPKTSAVFRQTPQIFRESVEEYMKEIKEVSYKVLEMVAEELGIEPRDTLSKMLRDEK 180

Query: 181 ADCYFRVNHYPPCPEMQAVGGRNMIGFGEHTDPQIISLLRSNNSTGLQICRRDGVWVSVP 240
           +D   R+NHYP   E         +GFGEHTDPQIIS+LRSNN+ GLQIC +DG WV+VP
Sbjct: 181 SDSCLRLNHYPAAEE--EAEKMVKVGFGEHTDPQIISVLRSNNTAGLQICVKDGSWVAVP 240

Query: 241 PDTTAFFVNVGDALQV 248
           PD ++FF+NVGDALQV
Sbjct: 241 PDHSSFFINVGDALQV 253

The following BLAST results are available for this feature:

BLAST of Carg19269 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7034720.1	1.8e-189	100.00	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
KAG6581428.1	1.2e-188	99.70	Gibberellin 2-beta-dioxygenase, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_022925964.1	2.1e-185	97.58	gibberellin 2-beta-dioxygenase [Cucurbita moschata]	[more]
CBL59329.1	3.6e-182	96.68	Gibberellin 2-oxidase [Cucurbita maxima]	[more]
XP_022977872.1	1.1e-181	96.37	gibberellin 2-beta-dioxygenase [Cucurbita maxima]	[more]

BLAST of Carg19269 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XG83	2.0e-114	61.56	Gibberellin 2-beta-dioxygenase OS=Phaseolus coccineus OX=3886 GN=GA2OX1 PE=2 SV=...	[more]
Q9SQ80	1.1e-104	55.18	Gibberellin 2-beta-dioxygenase 1 OS=Pisum sativum OX=3888 GN=GA2OX1 PE=1 SV=1	[more]
Q9XFR9	6.2e-100	55.39	Gibberellin 2-beta-dioxygenase 2 OS=Arabidopsis thaliana OX=3702 GN=GA2OX2 PE=2 ...	[more]
Q8LEA2	6.6e-94	53.21	Gibberellin 2-beta-dioxygenase 1 OS=Arabidopsis thaliana OX=3702 GN=GA2OX1 PE=2 ...	[more]
O64692	1.1e-93	52.55	Gibberellin 2-beta-dioxygenase 3 OS=Arabidopsis thaliana OX=3702 GN=GA2OX3 PE=2 ...	[more]

BLAST of Carg19269 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1EDN7	1.0e-185	97.58	gibberellin 2-beta-dioxygenase OS=Cucurbita moschata OX=3662 GN=LOC111433225 PE=...	[more]
I0J1J0	1.8e-182	96.68	Gibberellin 2-oxidase OS=Cucurbita maxima OX=3661 GN=ga2ox2 PE=2 SV=1	[more]
A0A6J1IJN1	5.1e-182	96.37	gibberellin 2-beta-dioxygenase OS=Cucurbita maxima OX=3661 GN=LOC111478033 PE=3 ...	[more]
A0A2H4V3T7	8.8e-158	82.18	Gibberellin 2-beta-dioxygenase 8-like protein OS=Luffa aegyptiaca OX=3670 PE=2 S...	[more]
A0A5A7UGB1	9.1e-155	82.42	Gibberellin 2-beta-dioxygenase-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5...	[more]

BLAST of Carg19269 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G30040.1	4.4e-101	55.39	gibberellin 2-oxidase	[more]
AT1G78440.1	4.7e-95	53.21	Arabidopsis thaliana gibberellin 2-oxidase 1	[more]
AT2G34555.1	8.0e-95	52.55	gibberellin 2-oxidase 3	[more]
AT1G02400.1	6.8e-70	43.30	gibberellin 2-oxidase 6	[more]
AT1G30040.2	3.4e-69	52.34	gibberellin 2-oxidase	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR044861	Isopenicillin N synthase-like, Fe(2+) 2OG dioxygenase domain	PFAM	PF03171	2OG-FeII_Oxy	coord: 176..275 e-value: 3.2E-25 score: 88.5
IPR026992	Non-haem dioxygenase N-terminal domain	PFAM	PF14226	DIOX_N	coord: 30..116 e-value: 2.2E-12 score: 47.7
IPR027443	Isopenicillin N synthase-like superfamily	GENE3D	2.60.120.330		coord: 10..318 e-value: 2.4E-75 score: 255.8
None	No IPR available	PANTHER	PTHR47990	2-OXOGLUTARATE (2OG) AND FE(II)-DEPENDENT OXYGENASE SUPERFAMILY PROTEIN-RELATED	coord: 10..326
None	No IPR available	PANTHER	PTHR47990:SF121	GIBBERELLIN 2-BETA-DIOXYGENASE 2	coord: 10..326
None	No IPR available	SUPERFAMILY	51197	Clavaminate synthase-like	coord: 24..317
IPR005123	Oxoglutarate/iron-dependent dioxygenase	PROSITE	PS51471	FE2OG_OXY	coord: 171..277 score: 11.707459

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19269-RA	Carg19269-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0051213	dioxygenase activity
molecular_function	GO:0046872	metal ion binding