Carg19229 (gene) Silver-seed gourd (SMH-JMG-627) v2

Overview


Name	Carg19229
Type	gene
Organism	Cucurbita argyrosperma subsp. argyrosperma cv. SMH-JMG-627 (Silver-seed gourd (SMH-JMG-627) v2)
Description	Epidermal patterning factor-like protein
Location	Carg_Chr19: 8456387 .. 8456717 (-)
RNA-Seq Expression	Carg19229
Synteny	Carg19229

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAAGGGTTCGACTTGGTTTTCTATTATCTTCATTGCTTTCTTACTTTTCCCAACACCCATTTCCTCACAACGCATTTCTCACCAGAACTCACGTAATCACTCATACCTTCAACACCCATTTCATGTCTTCAATGCTTTTCTTCGTTCCGCCATGAACTAACCTTCATGACTTGTGTGCAGGTCATGTCCACCACCGCAAGATGGCGGCAGAAGAGGCGGCGATGGGGAGAAAAGGGGTGGAGACAGTTGAAGTAGCAGGGTCGAGCTTGCCGGACTGCTCGCATGCGTGTGCCTCGTGCTCGCCGTGCAGACTAGTGATGATAAGTTTT

mRNA sequence

ATGAAGGGTTCGACTTGGTTTTCTATTATCTTCATTGCTTTCTTACTTTTCCCAACACCCATTTCCTCACAACGCATTTCTCACCAGAACTCACGTCATGTCCACCACCGCAAGATGGCGGCAGAAGAGGCGGCGATGGGGAGAAAAGGGGTGGAGACAGTTGAAGTAGCAGGGTCGAGCTTGCCGGACTGCTCGCATGCGTGTGCCTCGTGCTCGCCGTGCAGACTAGTGATGATAAGTTTT

Coding sequence (CDS)

ATGAAGGGTTCGACTTGGTTTTCTATTATCTTCATTGCTTTCTTACTTTTCCCAACACCCATTTCCTCACAACGCATTTCTCACCAGAACTCACGTCATGTCCACCACCGCAAGATGGCGGCAGAAGAGGCGGCGATGGGGAGAAAAGGGGTGGAGACAGTTGAAGTAGCAGGGTCGAGCTTGCCGGACTGCTCGCATGCGTGTGCCTCGTGCTCGCCGTGCAGACTAGTGATGATAAGTTTT

Protein sequence

MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF

Homology

[Close]

BLAST of Carg19229 vs. NCBI nr
Match: KAG7011966.1 (Protein EPIDERMAL PATTERNING FACTOR 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 164.9 bits (416), Expect = 3.0e-37
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS 60
          MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS
Sbjct: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS 60

Query: 61 LPDCSHACASCSPCRLVMISF 82
          LPDCSHACASCSPCRLVMISF
Sbjct: 61 LPDCSHACASCSPCRLVMISF 81

[Close]

BLAST of Carg19229 vs. NCBI nr
Match: KAG6572355.1 (Protein EPIDERMAL PATTERNING FACTOR 1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 164.9 bits (416), Expect = 3.0e-37
Identity = 81/81 (100.00%), Postives = 81/81 (100.00%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS 60
          MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS
Sbjct: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS 60

Query: 61 LPDCSHACASCSPCRLVMISF 82
          LPDCSHACASCSPCRLVMISF
Sbjct: 61 LPDCSHACASCSPCRLVMISF 81

[Close]

BLAST of Carg19229 vs. NCBI nr
Match: XP_022953138.1 (protein EPIDERMAL PATTERNING FACTOR 1 [Cucurbita moschata])

HSP 1 Score: 135.2 bits (339), Expect = 2.5e-28
Identity = 70/81 (86.42%), Postives = 71/81 (87.65%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS 60
          MKGSTWFSIIFIAFLLFPTPISSQRISHQNS          +EAAMGRKGVETVEVAGSS
Sbjct: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNS----------QEAAMGRKGVETVEVAGSS 60

Query: 61 LPDCSHACASCSPCRLVMISF 82
          LPDCSHACASCSPCRLVMISF
Sbjct: 61 LPDCSHACASCSPCRLVMISF 71

[Close]

BLAST of Carg19229 vs. NCBI nr
Match: XP_038888568.1 (protein EPIDERMAL PATTERNING FACTOR 1 [Benincasa hispida])

HSP 1 Score: 124.4 bits (311), Expect = 4.5e-25
Identity = 63/92 (68.48%), Postives = 70/92 (76.09%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAA-----------EEAAMGRK 60
          M  STWFSI+FIAF LFPTPISS+ IS +NS H HHRKMA            ++A +GRK
Sbjct: 1  MNPSTWFSILFIAFFLFPTPISSRHISQRNSHHGHHRKMATTTVKEKLYSPEKKAVIGRK 60

Query: 61 GVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          G +TVEVAGSSLPDCSHAC SCSPCRLVMISF
Sbjct: 61 GADTVEVAGSSLPDCSHACGSCSPCRLVMISF 92

[Close]

BLAST of Carg19229 vs. NCBI nr
Match: XP_004136818.1 (protein EPIDERMAL PATTERNING FACTOR 1 [Cucumis sativus] >KGN43602.1 hypothetical protein Csa_020445 [Cucumis sativus])

HSP 1 Score: 120.9 bits (302), Expect = 4.9e-24
Identity = 69/97 (71.13%), Postives = 72/97 (74.23%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAA------------EEAA--- 60
          MK  T FSI+FIAFLLF TPISS+RIS QNSRH HHRK AA            E+AA   
Sbjct: 1  MKPFTCFSILFIAFLLFSTPISSRRISQQNSRHGHHRKGAASSSGKAAMVVVKEKAAMMT 60

Query: 61 -MGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
           MGRKG ETVEVAGSSLPDCSHAC SCSPCRLVMISF
Sbjct: 61 RMGRKGTETVEVAGSSLPDCSHACGSCSPCRLVMISF 97

[Close]

BLAST of Carg19229 vs. ExPASy Swiss-Prot
Match: Q8S8I4 (Protein EPIDERMAL PATTERNING FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=EPF1 PE=1 SV=1)

HSP 1 Score: 59.7 bits (143), Expect = 1.8e-08
Identity = 29/52 (55.77%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 30 NSRHVHHRKMAAEEAAMGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          +S   HH           R+  +TV+VAGS LPDCSHAC SCSPCRLVM+SF
Sbjct: 26 SSHPSHHHVGMTGALKRQRRRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSF 77

[Close]

BLAST of Carg19229 vs. ExPASy Swiss-Prot
Match: Q8LC53 (Protein EPIDERMAL PATTERNING FACTOR 2 OS=Arabidopsis thaliana OX=3702 GN=EPF2 PE=1 SV=1)

HSP 1 Score: 47.0 bits (110), Expect = 1.2e-04
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 35 HHRKMAAEEAAMGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          HH+K  ++   +  +   T    GSSLPDCS+AC +CSPC+ VMISF
Sbjct: 51 HHKKEISKNGGVEMEMYPT----GSSLPDCSYACGACSPCKRVMISF 93

[Close]

BLAST of Carg19229 vs. ExPASy TrEMBL
Match: A0A6J1GMI9 (Epidermal patterning factor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111455631 PE=3 SV=1)

HSP 1 Score: 135.2 bits (339), Expect = 1.2e-28
Identity = 70/81 (86.42%), Postives = 71/81 (87.65%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEAAMGRKGVETVEVAGSS 60
          MKGSTWFSIIFIAFLLFPTPISSQRISHQNS          +EAAMGRKGVETVEVAGSS
Sbjct: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNS----------QEAAMGRKGVETVEVAGSS 60

Query: 61 LPDCSHACASCSPCRLVMISF 82
          LPDCSHACASCSPCRLVMISF
Sbjct: 61 LPDCSHACASCSPCRLVMISF 71

[Close]

BLAST of Carg19229 vs. ExPASy TrEMBL
Match: A0A0A0K5P8 (Epidermal patterning factor-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G047370 PE=3 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 2.4e-24
Identity = 69/97 (71.13%), Postives = 72/97 (74.23%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAA------------EEAA--- 60
          MK  T FSI+FIAFLLF TPISS+RIS QNSRH HHRK AA            E+AA   
Sbjct: 1  MKPFTCFSILFIAFLLFSTPISSRRISQQNSRHGHHRKGAASSSGKAAMVVVKEKAAMMT 60

Query: 61 -MGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
           MGRKG ETVEVAGSSLPDCSHAC SCSPCRLVMISF
Sbjct: 61 RMGRKGTETVEVAGSSLPDCSHACGSCSPCRLVMISF 97

[Close]

BLAST of Carg19229 vs. ExPASy TrEMBL
Match: A0A1S3C0N9 (Epidermal patterning factor-like protein OS=Cucumis melo OX=3656 GN=LOC103495522 PE=3 SV=1)

HSP 1 Score: 116.7 bits (291), Expect = 4.5e-23
Identity = 65/95 (68.42%), Postives = 66/95 (69.47%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEA--------------AM 60
          MK  T FSIIFI FLL  TPISS+RIS QNSRH HHRK AA                  M
Sbjct: 1  MKPFTCFSIIFIGFLLSSTPISSRRISQQNSRHGHHRKGAASSGKAAMVVVNEKVTMMTM 60

Query: 61 GRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          GRKG ETVEVAGSSLPDCSHAC SCSPCRLVMISF
Sbjct: 61 GRKGTETVEVAGSSLPDCSHACGSCSPCRLVMISF 95

[Close]

BLAST of Carg19229 vs. ExPASy TrEMBL
Match: A0A6J1ESJ9 (Epidermal patterning factor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111437184 PE=3 SV=1)

HSP 1 Score: 112.5 bits (280), Expect = 8.5e-22
Identity = 63/102 (61.76%), Postives = 66/102 (64.71%), Query Frame = 0


Query: 1   MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEE----------------- 60
           MKG   FS+IFIAFLLFP PISS+RIS  NSR  HH K A +                  
Sbjct: 1   MKGLPCFSLIFIAFLLFPIPISSRRISQLNSRRGHHHKAAVDSVRAVEAAKGKSYWPVTK 60

Query: 61  ----AAMGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
               A MGRKG ETVEVAGSSLPDCSHAC SCSPCRLVMISF
Sbjct: 61  AVAAAVMGRKGPETVEVAGSSLPDCSHACGSCSPCRLVMISF 102

[Close]

BLAST of Carg19229 vs. ExPASy TrEMBL
Match: A0A6J1JEL1 (Epidermal patterning factor-like protein OS=Cucurbita maxima OX=3661 GN=LOC111486204 PE=3 SV=1)

HSP 1 Score: 111.3 bits (277), Expect = 1.9e-21
Identity = 61/94 (64.89%), Postives = 65/94 (69.15%), Query Frame = 0


Query: 1  MKGSTWFSIIFIAFLLFPTPISSQRISHQNSRHVHHRKMAAEEA-------------AMG 60
          MKG   FS+IFIAFLLFP PISS+RIS  NSR  HH K A +               A+ 
Sbjct: 1  MKGLPCFSLIFIAFLLFPIPISSRRISQLNSRRGHHHKAAVDSVRAVEAAKQNSYWPALW 60

Query: 61 RKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          RKG ETVEVAGSSLPDCSHAC SCSPCRLVMISF
Sbjct: 61 RKGPETVEVAGSSLPDCSHACGSCSPCRLVMISF 94

[Close]

BLAST of Carg19229 vs. TAIR 10
Match: AT2G20875.1 (epidermal patterning factor 1 )

HSP 1 Score: 59.7 bits (143), Expect = 1.3e-09
Identity = 29/52 (55.77%), Postives = 34/52 (65.38%), Query Frame = 0


Query: 30 NSRHVHHRKMAAEEAAMGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          +S   HH           R+  +TV+VAGS LPDCSHAC SCSPCRLVM+SF
Sbjct: 26 SSHPSHHHVGMTGALKRQRRRPDTVQVAGSRLPDCSHACGSCSPCRLVMVSF 77

[Close]

BLAST of Carg19229 vs. TAIR 10
Match: AT1G34245.1 (Putative membrane lipoprotein )

HSP 1 Score: 47.0 bits (110), Expect = 8.5e-06
Identity = 23/47 (48.94%), Postives = 31/47 (65.96%), Query Frame = 0


Query: 35 HHRKMAAEEAAMGRKGVETVEVAGSSLPDCSHACASCSPCRLVMISF 82
          HH+K  ++   +  +   T    GSSLPDCS+AC +CSPC+ VMISF
Sbjct: 51 HHKKEISKNGGVEMEMYPT----GSSLPDCSYACGACSPCKRVMISF 93

[Close]

BLAST of Carg19229 vs. TAIR 10
Match: AT1G71866.1 (LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: Putative membrane lipoprotein (TAIR:AT1G34245.1). )

HSP 1 Score: 42.0 bits (97), Expect = 2.7e-04
Identity = 16/26 (61.54%), Postives = 22/26 (84.62%), Query Frame = 0


Query: 55  EVAGSSLPDCSHACASCSPCRLVMIS 81
           + +GSS+PDCS+AC  C PC+LV+IS
Sbjct: 119 KASGSSIPDCSNACGPCKPCKLVVIS 144

The following BLAST results are available for this feature:

BLAST of Carg19229 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG7011966.1	3.0e-37	100.00	Protein EPIDERMAL PATTERNING FACTOR 1, partial [Cucurbita argyrosperma subsp. ar...	[more]
KAG6572355.1	3.0e-37	100.00	Protein EPIDERMAL PATTERNING FACTOR 1, partial [Cucurbita argyrosperma subsp. so...	[more]
XP_022953138.1	2.5e-28	86.42	protein EPIDERMAL PATTERNING FACTOR 1 [Cucurbita moschata]	[more]
XP_038888568.1	4.5e-25	68.48	protein EPIDERMAL PATTERNING FACTOR 1 [Benincasa hispida]	[more]
XP_004136818.1	4.9e-24	71.13	protein EPIDERMAL PATTERNING FACTOR 1 [Cucumis sativus] >KGN43602.1 hypothetical...	[more]

BLAST of Carg19229 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8S8I4	1.8e-08	55.77	Protein EPIDERMAL PATTERNING FACTOR 1 OS=Arabidopsis thaliana OX=3702 GN=EPF1 PE...	[more]
Q8LC53	1.2e-04	48.94	Protein EPIDERMAL PATTERNING FACTOR 2 OS=Arabidopsis thaliana OX=3702 GN=EPF2 PE...	[more]

BLAST of Carg19229 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1GMI9	1.2e-28	86.42	Epidermal patterning factor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A0A0K5P8	2.4e-24	71.13	Epidermal patterning factor-like protein OS=Cucumis sativus OX=3659 GN=Csa_7G047...	[more]
A0A1S3C0N9	4.5e-23	68.42	Epidermal patterning factor-like protein OS=Cucumis melo OX=3656 GN=LOC103495522...	[more]
A0A6J1ESJ9	8.5e-22	61.76	Epidermal patterning factor-like protein OS=Cucurbita moschata OX=3662 GN=LOC111...	[more]
A0A6J1JEL1	1.9e-21	64.89	Epidermal patterning factor-like protein OS=Cucurbita maxima OX=3661 GN=LOC11148...	[more]

BLAST of Carg19229 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20875.1	1.3e-09	55.77	epidermal patterning factor 1	[more]
AT1G34245.1	8.5e-06	48.94	Putative membrane lipoprotein	[more]
AT1G71866.1	2.7e-04	61.54	LOCATED IN: endomembrane system; BEST Arabidopsis thaliana protein match is: Put...	[more]

InterPro

Analysis Name: InterPro Annotations of Silver-seed gourd (SMH-JMG-627) v2
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF17181	EPF	coord: 57..79 e-value: 1.2E-8 score: 34.8
None	No IPR available	PANTHER	PTHR33109:SF41	PROTEIN EPIDERMAL PATTERNING FACTOR 1	coord: 18..80
IPR039455	EPIDERMAL PATTERNING FACTOR-like protein	PANTHER	PTHR33109	EPIDERMAL PATTERNING FACTOR-LIKE PROTEIN 4	coord: 18..80

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
Carg19229-RA	Carg19229-RA	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0010052	guard cell differentiation
biological_process	GO:0010374	stomatal complex development
cellular_component	GO:0005576	extracellular region